Belongs to the SNF7 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 2 cut(s) | 208, 298 |
| AcsI | RAATTY | 3 cut(s) | 77, 95, 181 |
| AgsI | TTSAA | 4 cut(s) | 77, 83, 139, 157 |
| AjiI | CACGTC | 1 cut(s) | 268 |
| AjnI | CCWGG | 1 cut(s) | 306 |
| AluBI | AGCT | 2 cut(s) | 314, 338 |
| AluI | AGCT | 2 cut(s) | 314, 338 |
| Alw26I | GTCTC | 1 cut(s) | 14 |
| AlwI | GGATC | 2 cut(s) | 208, 298 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 341 |
| AoxI | GGCC | 1 cut(s) | 87 |
| ApeKI | GCWGC | 2 cut(s) | 68, 338 |
| ApoI | RAATTY | 3 cut(s) | 77, 95, 181 |
| AseI | ATTAAT | 1 cut(s) | 291 |
| AspS9I | GGNCC | 1 cut(s) | 87 |
| AsuHPI | GGTGA | 1 cut(s) | 236 |
| BbvI | GCAGC | 2 cut(s) | 55, 325 |
| BciT130I | CCWGG | 1 cut(s) | 308 |
| BcoDI | GTCTC | 1 cut(s) | 14 |
| BfaI | CTAG | 1 cut(s) | 56 |
| BisI | GCNGC | 2 cut(s) | 69, 339 |
| BlsI | GCNGC | 2 cut(s) | 70, 340 |
| Bme1390I | CCNGG | 1 cut(s) | 308 |
| BmgBI | CACGTC | 1 cut(s) | 268 |
| BmgT120I | GGNCC | 1 cut(s) | 87 |
| BmrFI | CCNGG | 1 cut(s) | 308 |
| BmsI | GCATC | 2 cut(s) | 21, 346 |
| BpuEI | CTTGAG | 1 cut(s) | 56 |
| BsaBI | GATNNNNATC | 1 cut(s) | 212 |
| Bse3DI | GCAATG | 1 cut(s) | 246 |
| Bse8I | GATNNNNATC | 1 cut(s) | 212 |
| BseBI | CCWGG | 1 cut(s) | 308 |
| BseGI | GGATG | 2 cut(s) | 36, 156 |
| BseJI | GATNNNNATC | 1 cut(s) | 212 |
| BseMI | GCAATG | 1 cut(s) | 246 |
| BseXI | GCAGC | 2 cut(s) | 55, 325 |
| BshFI | GGCC | 1 cut(s) | 89 |
| BsmAI | GTCTC | 1 cut(s) | 14 |
| BsmBI | CGTCTC | 1 cut(s) | 14 |
| BsnI | GGCC | 1 cut(s) | 89 |
| Bsp143I | GATC | 3 cut(s) | 48, 213, 303 |
| BspANI | GGCC | 1 cut(s) | 89 |
| BspPI | GGATC | 2 cut(s) | 208, 298 |
| BsrDI | GCAATG | 1 cut(s) | 246 |
| BssMI | GATC | 3 cut(s) | 48, 213, 303 |
| Bst2UI | CCWGG | 1 cut(s) | 308 |
| Bst4CI | ACNGT | 1 cut(s) | 280 |
| Bst6I | CTCTTC | 2 cut(s) | 134, 140 |
| BstF5I | GGATG | 2 cut(s) | 36, 156 |
| BstKTI | GATC | 3 cut(s) | 51, 216, 306 |
| BstMAI | GTCTC | 1 cut(s) | 14 |
| BstMBI | GATC | 3 cut(s) | 48, 213, 303 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 31 |
| BstNI | CCWGG | 1 cut(s) | 308 |
| BstSCI | CCNGG | 1 cut(s) | 306 |
| BstV1I | GCAGC | 2 cut(s) | 55, 325 |
| BstX2I | RGATCY | 1 cut(s) | 303 |
| BstYI | RGATCY | 1 cut(s) | 303 |
| BsuRI | GGCC | 1 cut(s) | 89 |
| BtrI | CACGTC | 1 cut(s) | 268 |
| BtsCI | GGATG | 2 cut(s) | 36, 156 |
| CaiI | CAGNNNCTG | 1 cut(s) | 341 |
| Cfr13I | GGNCC | 1 cut(s) | 87 |
| CseI | GACGC | 1 cut(s) | 31 |
| CviAII | CATG | 1 cut(s) | 17 |
| CviJI | RGCY | 4 cut(s) | 68, 89, 314, 338 |
| CviKI_1 | RGCY | 4 cut(s) | 68, 89, 314, 338 |
| DpnI | GATC | 3 cut(s) | 50, 215, 305 |
| DpnII | GATC | 3 cut(s) | 48, 213, 303 |
| Eam1104I | CTCTTC | 2 cut(s) | 134, 140 |
| EarI | CTCTTC | 2 cut(s) | 134, 140 |
| EcoRI | GAATTC | 1 cut(s) | 95 |
| EcoRII | CCWGG | 1 cut(s) | 306 |
| EcoT22I | ATGCAT | 1 cut(s) | 361 |
| Esp3I | CGTCTC | 1 cut(s) | 14 |
| FaeI | CATG | 1 cut(s) | 20 |
| FaiI | YATR | 3 cut(s) | 18, 128, 201 |
| FatI | CATG | 1 cut(s) | 16 |
| Fnu4HI | GCNGC | 2 cut(s) | 69, 339 |
| FokI | GGATG | 2 cut(s) | 43, 163 |
| Fsp4HI | GCNGC | 2 cut(s) | 69, 339 |
| FspBI | CTAG | 1 cut(s) | 56 |
| GluI | GCNGC | 2 cut(s) | 69, 339 |
| HaeIII | GGCC | 1 cut(s) | 89 |
| HgaI | GACGC | 1 cut(s) | 31 |
| Hin1II | CATG | 1 cut(s) | 20 |
| HincII | GTYRAC | 1 cut(s) | 321 |
| HindII | GTYRAC | 1 cut(s) | 321 |
| HindIII | AAGCTT | 1 cut(s) | 312 |
| HinfI | GANTC | 1 cut(s) | 195 |
| HphI | GGTGA | 1 cut(s) | 236 |
| Hpy166II | GTNNAC | 1 cut(s) | 321 |
| Hpy188I | TCNGA | 3 cut(s) | 53, 188, 207 |
| Hpy8I | GTNNAC | 1 cut(s) | 321 |
| HpyAV | CCTTC | 2 cut(s) | 58, 229 |
| HpyCH4III | ACNGT | 1 cut(s) | 280 |
| HpyCH4IV | ACGT | 1 cut(s) | 267 |
| HpyCH4V | TGCA | 2 cut(s) | 244, 359 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 31 |
| HpySE526I | ACGT | 1 cut(s) | 267 |
| Hsp92II | CATG | 1 cut(s) | 20 |
| Kzo9I | GATC | 3 cut(s) | 48, 213, 303 |
| LpnPI | CCDG | 4 cut(s) | 115, 133, 293, 320 |
| Lsp1109I | GCAGC | 2 cut(s) | 55, 325 |
| LweI | GCATC | 2 cut(s) | 21, 346 |
| MaeI | CTAG | 1 cut(s) | 56 |
| MaeII | ACGT | 1 cut(s) | 267 |
| MaeIII | GTNAC | 1 cut(s) | 12 |
| MalI | GATC | 3 cut(s) | 50, 215, 305 |
| MboI | GATC | 3 cut(s) | 48, 213, 303 |
| MboII | GAAGA | 3 cut(s) | 73, 151, 157 |
| MfeI | CAATTG | 1 cut(s) | 327 |
| MflI | RGATCY | 1 cut(s) | 303 |
| MluCI | AATT | 4 cut(s) | 77, 95, 181, 327 |
| MmeI | TCCRAC | 1 cut(s) | 157 |
| MnlI | CCTC | 2 cut(s) | 135, 141 |
| Mph1103I | ATGCAT | 1 cut(s) | 361 |
| MseI | TTAA | 1 cut(s) | 291 |
| MspA1I | CMGCKG | 1 cut(s) | 338 |
| MspR9I | CCNGG | 1 cut(s) | 308 |
| MunI | CAATTG | 1 cut(s) | 327 |
| MvaI | CCWGG | 1 cut(s) | 308 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 31 |
| NdeII | GATC | 3 cut(s) | 48, 213, 303 |
| NlaIII | CATG | 1 cut(s) | 20 |
| NsiI | ATGCAT | 1 cut(s) | 361 |
| PfeI | GAWTC | 1 cut(s) | 195 |
| PfoI | TCCNGGA | 1 cut(s) | 306 |
| PkrI | GCNGC | 2 cut(s) | 70, 340 |
| PshBI | ATTAAT | 1 cut(s) | 291 |
| Psp6I | CCWGG | 1 cut(s) | 306 |
| PspGI | CCWGG | 1 cut(s) | 306 |
| PspPI | GGNCC | 1 cut(s) | 87 |
| PstNI | CAGNNNCTG | 1 cut(s) | 341 |
| PsuI | RGATCY | 1 cut(s) | 303 |
| PvuII | CAGCTG | 1 cut(s) | 338 |
| SaqAI | TTAA | 1 cut(s) | 291 |
| SatI | GCNGC | 2 cut(s) | 69, 339 |
| Sau3AI | GATC | 3 cut(s) | 48, 213, 303 |
| Sau96I | GGNCC | 1 cut(s) | 87 |
| ScrFI | CCNGG | 1 cut(s) | 308 |
| SetI | ASST | 5 cut(s) | 122, 226, 270, 316, 340 |
| SfaNI | GCATC | 2 cut(s) | 21, 346 |
| SmlI | CTYRAG | 1 cut(s) | 35 |
| SmoI | CTYRAG | 1 cut(s) | 35 |
| Sse9I | AATT | 4 cut(s) | 77, 95, 181, 327 |
| SspMI | CTAG | 1 cut(s) | 56 |
| StyD4I | CCNGG | 1 cut(s) | 306 |
| TaaI | ACNGT | 1 cut(s) | 280 |
| TaiI | ACGT | 1 cut(s) | 270 |
| TasI | AATT | 4 cut(s) | 77, 95, 181, 327 |
| TfiI | GAWTC | 1 cut(s) | 195 |
| Tru1I | TTAA | 1 cut(s) | 291 |
| Tru9I | TTAA | 1 cut(s) | 291 |
| TseI | GCWGC | 2 cut(s) | 68, 338 |
| TspDTI | ATGAA | 1 cut(s) | 248 |
| VspI | ATTAAT | 1 cut(s) | 291 |
| XapI | RAATTY | 3 cut(s) | 77, 95, 181 |
| XspI | CTAG | 1 cut(s) | 56 |
| Zsp2I | ATGCAT | 1 cut(s) | 361 |