Rh5BG025500

Paired amphipathic helix protein Sin3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001478
Physical Location & Seq
Forward (+)
1799877 .. 1801295
1419 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG025500.1

Sequence Viewer

Length: 1419 bp
ATGGTTCCACTACCCGAAAAAGAGTATTCCGCAGAACCGCGGATGATGAAGGAGAAGAGCGCAGGAGAAGAGGCCAGCAGCACCAAAGCGTCGTGGGAGAAGCGCTCCCTCGAATTTATTGACTCCTTGAGGAACTTCCCATGGTTCAAGGAGTATCAGAACCTCATGAAAGACTACAAGAACAAAGAAATCGACTTGGCAATTTTGGTGGAGAAGATGGTGGAATTGTTTAAAGGGCGCCCGGAGTTGATAGCAGGGTTCAATAAATTCTTGCCAGAAACATATCAAATTACCATACCAGCACCAACGCTACTACCTAGGGTTAGAATAGTTTGTCAAAAGAGGAAGCGCAGCGATGATAGTGCTAGTGATAGTGATGGTGGAGCTAAGGACTCTAAGATTCGAAAAGTTATCAGGTGTGATTACAAGACCAAGGGAAGGTTTCACGACGAAGATAAGGAAAGTGATGAAGGTCACCATGTTTTCTTGGGACACGAATCCCCAACTCTGTCACCAAGACCTAGGATTACAGTTCTTTCAAACAAGGCGAAGAACGATGATGATAGTACCACTGATTGTGATGACGCTGGATCTATAGATTCTAAACTTCGGAAAGTTATTGCTTTTGTGAACAAGGTCGAGGAAAGGTTCCGCAGCAACAATGATGATGGTGGTGACGATGATGATACTTGTCAATCCCATATAGAGTTTTTCTTGGATATGGTCTGCAAAAGAAACAAATCATCAGCCAATAGATCTATCGATGCTGATGAAGAAGAGCTTCAGATTTACAAGAAGGTTGCAACACTTTTGGAAGGCCACTCGGACTTAATTGATGAGTTCACCCAGTTTTTAACCGAAGTGCCTAGAGTCGACTCCATCATTGATTTCCGTAAGTGCAAGACTTGCACTCCAAGCTACCGTCTTTTGCCAAAAAATTTCCCCATTCCTGCTGCTACCAAAAGATCGAAGCTAGCTTCTGAAGTATTAAATGATCGTTGGGTATGCGTTAACACTAGTCCTAGTACTACTAGTTCTACTTCAAGAGGAGGTGGACGACGCAAAGACCGCAAATCGCAGCATGAGCAAGACCTATTAGACTGGGAAGAAGAAAAGTATGAACATGATATGTTGGAAGAGTATGTGAATTCGGCCGCAAATAATATCGCAAGGCTAATAAAAGAGATCAAAAACGAGAGTCCGACGGAGATTTATCTTGAAGAACATCTCACGGAGATGAATTTGAGGTGCATTGAGAGATTGTATGGTCTGCTAGGGCGTGATATGGTTGATTTGTTGAAGAACAATATTCAAGCGGTTTTGCCTGTTATATTAAGACGGTTGAAGCAAAAGCAGAAAGAACTGAAACGAAAACGTTCTGATCTAAAACAAGAGTGGGCTCATTTATTTCCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04139 ko04919 ko05016 ko05202 map04139 map04919 map05016 map05202
Pfam Domains
Protein Families
PAH Sin3_corepress Sin3a_C

Protein Analysis

472

Amino Acids

54.6

Weight (kDa)

6.29

Isoelectric Point (pI)

59.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAH PF02671 51 - 91 1.5e-09 Paired amphipathic helix repeat
Sin3_corepress PF08295 301 - 402 8e-20 Sin3 family co-repressor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 237
AccI GTMKAC 1 cut(s) 873
AccII CGCG 1 cut(s) 40
AciI CCGC 7 cut(s) 30, 38, 40, 652, 1069, 1155, 1316
AclI AACGTT 1 cut(s) 1375
AclWI GGATC 1 cut(s) 598
AcoI YGGCCR 1 cut(s) 1152
AcsI RAATTY 5 cut(s) 113, 266, 937, 1147, 1240
AcuI CTGAAG 2 cut(s) 767, 1002
AcyI GRCGYC 1 cut(s) 238
AfaI GTAC 2 cut(s) 568, 1027
AfeI AGCGCT 1 cut(s) 104
AfiI CCNNNNNNNGG 1 cut(s) 438
AgsI TTSAA 8 cut(s) 148, 262, 540, 1044, 1220, 1300, 1313, 1345
AhlI ACTAGT 2 cut(s) 1016, 1031
AluBI AGCT 5 cut(s) 386, 781, 918, 973, 977
AluI AGCT 5 cut(s) 386, 781, 918, 973, 977
AlwI GGATC 1 cut(s) 598
Aor51HI AGCGCT 1 cut(s) 104
AoxI GGCC 3 cut(s) 72, 817, 1152
ApeKI GCWGC 5 cut(s) 78, 351, 654, 953, 1078
ApoI RAATTY 5 cut(s) 113, 266, 937, 1147, 1240
Asp700I GAANNNNTTC 2 cut(s) 780, 1375
AspA2I CCTAGG 2 cut(s) 317, 521
AspLEI GCGC 4 cut(s) 62, 105, 240, 351
AsuC2I CCSGG 1 cut(s) 242
AsuHPI GGTGA 4 cut(s) 467, 504, 686, 835
AsuII TTCGAA 1 cut(s) 403
AsuNHI GCTAGC 1 cut(s) 973
AvrII CCTAGG 2 cut(s) 317, 521
BanI GGYRCC 1 cut(s) 237
BanII GRGCYC 1 cut(s) 1402
BbvI GCAGC 5 cut(s) 90, 363, 666, 940, 1090
BccI CCATC 4 cut(s) 211, 371, 662, 887
BcgI CGANNNNNNTGC 2 cut(s) 344, 378
BcnI CCSGG 1 cut(s) 242
BcuI ACTAGT 2 cut(s) 1016, 1031
BfaI CTAG 9 cut(s) 318, 366, 522, 867, 974, 1017, 1023, 1032, 1274
BfmI CTRYAG 1 cut(s) 594
BfoI RGCGCY 2 cut(s) 106, 241
BglII AGATCT 1 cut(s) 755
BisI GCNGC 6 cut(s) 79, 352, 655, 954, 1079, 1155
BlnI CCTAGG 2 cut(s) 317, 521
BlsI GCNGC 6 cut(s) 80, 353, 656, 955, 1080, 1156
BmcAI AGTACT 1 cut(s) 1027
Bme1390I CCNGG 1 cut(s) 242
BmiI GGNNCC 3 cut(s) 6, 239, 650
BmrFI CCNGG 1 cut(s) 242
BmrI ACTGGG 2 cut(s) 841, 1111
BmsI GCATC 1 cut(s) 754
BmtI GCTAGC 1 cut(s) 977
BmuI ACTGGG 2 cut(s) 841, 1111
BplI GAGNNNNNCTC 2 cut(s) 89, 121
Bpu10I CCTNAGC 1 cut(s) 387
Bpu14I TTCGAA 1 cut(s) 403
BpuEI CTTGAG 1 cut(s) 148
BpuMI CCSGG 1 cut(s) 242
Bsa29I ATCGAT 1 cut(s) 762
BsaHI GRCGYC 1 cut(s) 238
BsaJI CCNNGG 5 cut(s) 38, 140, 317, 432, 521
BsaXI ACNNNNNCTCC 2 cut(s) 895, 925
Bsc4I CCNNNNNNNGG 1 cut(s) 438
Bse1I ACTGG 2 cut(s) 847, 1106
BseCI ATCGAT 1 cut(s) 762
BseDI CCNNGG 5 cut(s) 38, 140, 317, 432, 521
BseGI GGATG 1 cut(s) 48
BseLI CCNNNNNNNGG 1 cut(s) 438
BseMII CTCAG 1 cut(s) 1404
BseNI ACTGG 2 cut(s) 847, 1106
BseRI GAGGAG 1 cut(s) 1062
BseX3I CGGCCG 1 cut(s) 1152
BseXI GCAGC 5 cut(s) 90, 363, 666, 940, 1090
Bsh1236I CGCG 1 cut(s) 40
Bsh1285I CGRYCG 1 cut(s) 1155
BshFI GGCC 3 cut(s) 74, 819, 1154
BshNI GGYRCC 1 cut(s) 237
BshVI ATCGAT 1 cut(s) 762
BsiEI CGRYCG 1 cut(s) 1155
BsiSI CCGG 1 cut(s) 242
BslFI GGGAC 1 cut(s) 504
BslI CCNNNNNNNGG 1 cut(s) 438
BsmFI GGGAC 1 cut(s) 504
BsnI GGCC 3 cut(s) 74, 819, 1154
Bsp119I TTCGAA 1 cut(s) 403
Bsp1286I GDGCHC 1 cut(s) 1402
Bsp143I GATC 6 cut(s) 590, 755, 965, 994, 1185, 1381
Bsp19I CCATGG 1 cut(s) 140
BspACI CCGC 7 cut(s) 30, 38, 40, 652, 1069, 1155, 1316
BspANI GGCC 3 cut(s) 74, 819, 1154
BspCNI CTCAG 1 cut(s) 1405
BspDI ATCGAT 1 cut(s) 762
BspFNI CGCG 1 cut(s) 40
BspHI TCATGA 1 cut(s) 165
BspLI GGNNCC 3 cut(s) 6, 239, 650
BspOI GCTAGC 1 cut(s) 977
BspPI GGATC 1 cut(s) 598
BspQI GCTCTTC 2 cut(s) 50, 771
BspT104I TTCGAA 1 cut(s) 403
BspT107I GGYRCC 1 cut(s) 237
BsrI ACTGG 2 cut(s) 847, 1106
BssECI CCNNGG 5 cut(s) 38, 140, 317, 432, 521
BssMI GATC 6 cut(s) 590, 755, 965, 994, 1185, 1381
BssNI GRCGYC 1 cut(s) 238
BssT1I CCWWGG 4 cut(s) 140, 317, 432, 521
Bst4CI ACNGT 3 cut(s) 532, 923, 1341
Bst6I CTCTTC 4 cut(s) 50, 63, 771, 1131
BstACI GRCGYC 1 cut(s) 238
BstAPI GCANNNNNTGC 1 cut(s) 906
BstBI TTCGAA 1 cut(s) 403
BstC8I GCNNGC 2 cut(s) 76, 975
BstDEI CTNAG 3 cut(s) 387, 396, 1413
BstDSI CCRYGG 2 cut(s) 38, 140
BstEII GGTNACC 1 cut(s) 473
BstF5I GGATG 1 cut(s) 48
BstFNI CGCG 1 cut(s) 40
BstH2I RGCGCY 2 cut(s) 106, 241
BstHHI GCGC 4 cut(s) 62, 105, 240, 351
BstKTI GATC 6 cut(s) 593, 758, 968, 997, 1188, 1384
BstMBI GATC 6 cut(s) 590, 755, 965, 994, 1185, 1381
BstMCI CGRYCG 1 cut(s) 1155
BstMWI GCNNNNNNNGC 4 cut(s) 906, 915, 1068, 1084
BstPI GGTNACC 1 cut(s) 473
BstSCI CCNGG 1 cut(s) 240
BstSFI CTRYAG 1 cut(s) 594
BstUI CGCG 1 cut(s) 40
BstV1I GCAGC 5 cut(s) 90, 363, 666, 940, 1090
BstX2I RGATCY 2 cut(s) 590, 755
BstYI RGATCY 2 cut(s) 590, 755
BstZI CGGCCG 1 cut(s) 1152
Bsu15I ATCGAT 1 cut(s) 762
BsuRI GGCC 3 cut(s) 74, 819, 1154
BsuTUI ATCGAT 1 cut(s) 762
BtgI CCRYGG 2 cut(s) 38, 140
BtgZI GCGATG 1 cut(s) 369
BtsCI GGATG 1 cut(s) 48
BtsIMutI CAGTG 1 cut(s) 570
Cac8I GCNNGC 2 cut(s) 76, 975
CciI TCATGA 1 cut(s) 165
CfoI GCGC 4 cut(s) 62, 105, 240, 351
Cfr42I CCGCGG 1 cut(s) 41
ClaI ATCGAT 1 cut(s) 762
CseI GACGC 3 cut(s) 78, 593, 1068
Csp6I GTAC 2 cut(s) 567, 1026
CspCI CAANNNNNGTGG 2 cut(s) 189, 224
CviAII CATG 5 cut(s) 141, 166, 479, 1082, 1124
CviQI GTAC 2 cut(s) 567, 1026
DdeI CTNAG 3 cut(s) 387, 396, 1413
DinI GGCGCC 1 cut(s) 239
DpnI GATC 6 cut(s) 592, 757, 967, 996, 1187, 1383
DpnII GATC 6 cut(s) 590, 755, 965, 994, 1185, 1381
DraI TTTAAA 1 cut(s) 232
EaeI YGGCCR 1 cut(s) 1152
EagI CGGCCG 1 cut(s) 1152
Eam1104I CTCTTC 4 cut(s) 50, 63, 771, 1131
EarI CTCTTC 4 cut(s) 50, 63, 771, 1131
EclXI CGGCCG 1 cut(s) 1152
Eco130I CCWWGG 4 cut(s) 140, 317, 432, 521
Eco24I GRGCYC 1 cut(s) 1402
Eco47III AGCGCT 1 cut(s) 104
Eco52I CGGCCG 1 cut(s) 1152
Eco57I CTGAAG 2 cut(s) 767, 1002
Eco91I GGTNACC 1 cut(s) 473
EcoO65I GGTNACC 1 cut(s) 473
EcoRI GAATTC 1 cut(s) 1147
EcoT14I CCWWGG 4 cut(s) 140, 317, 432, 521
EcoT38I GRGCYC 1 cut(s) 1402
EgeI GGCGCC 1 cut(s) 239
EheI GGCGCC 1 cut(s) 239
ErhI CCWWGG 4 cut(s) 140, 317, 432, 521
FaeI CATG 5 cut(s) 144, 169, 482, 1085, 1127
FalI AAGNNNNNCTT 2 cut(s) 765, 797
FaqI GGGAC 1 cut(s) 504
FatI CATG 5 cut(s) 140, 165, 478, 1081, 1123
FblI GTMKAC 1 cut(s) 873
Fnu4HI GCNGC 6 cut(s) 79, 352, 655, 954, 1079, 1155
FokI GGATG 1 cut(s) 55
FriOI GRGCYC 1 cut(s) 1402
Fsp4HI GCNGC 6 cut(s) 79, 352, 655, 954, 1079, 1155
FspBI CTAG 9 cut(s) 318, 366, 522, 867, 974, 1017, 1023, 1032, 1274
GlaI GCGC 4 cut(s) 61, 104, 239, 350
GluI GCNGC 6 cut(s) 79, 352, 655, 954, 1079, 1155
HaeII RGCGCY 2 cut(s) 106, 241
HaeIII GGCC 3 cut(s) 74, 819, 1154
HapII CCGG 1 cut(s) 242
HgaI GACGC 3 cut(s) 78, 593, 1068
HhaI GCGC 4 cut(s) 62, 105, 240, 351
Hin1I GRCGYC 1 cut(s) 238
Hin1II CATG 5 cut(s) 144, 169, 482, 1085, 1127
Hin6I GCGC 4 cut(s) 60, 103, 238, 349
HinP1I GCGC 4 cut(s) 60, 103, 238, 349
HincII GTYRAC 2 cut(s) 874, 1012
HindII GTYRAC 2 cut(s) 874, 1012
HinfI GANTC 8 cut(s) 122, 392, 400, 497, 599, 870, 875, 1198
HpaI GTTAAC 1 cut(s) 1012
HpaII CCGG 1 cut(s) 242
HphI GGTGA 4 cut(s) 467, 504, 686, 835
Hpy166II GTNNAC 5 cut(s) 631, 843, 874, 1012, 1055
Hpy188I TCNGA 7 cut(s) 159, 612, 786, 826, 982, 1203, 1381
Hpy188III TCNNGA 5 cut(s) 166, 446, 1044, 1217, 1412
Hpy8I GTNNAC 5 cut(s) 631, 843, 874, 1012, 1055
Hpy99I CGWCG 4 cut(s) 94, 452, 1062, 1207
HpyAV CCTTC 5 cut(s) 43, 432, 464, 790, 809
HpyCH4III ACNGT 3 cut(s) 532, 923, 1341
HpyCH4IV ACGT 1 cut(s) 1375
HpyCH4V TGCA 5 cut(s) 729, 803, 900, 909, 1251
HpyF10VI GCNNNNNNNGC 4 cut(s) 906, 915, 1068, 1084
HpyF3I CTNAG 3 cut(s) 387, 396, 1413
HpySE526I ACGT 1 cut(s) 1375
Hsp92I GRCGYC 1 cut(s) 238
Hsp92II CATG 5 cut(s) 144, 169, 482, 1085, 1127
HspAI GCGC 4 cut(s) 60, 103, 238, 349
KasI GGCGCC 1 cut(s) 237
KspAI GTTAAC 1 cut(s) 1012
KspI CCGCGG 1 cut(s) 41
Kzo9I GATC 6 cut(s) 590, 755, 965, 994, 1185, 1381
LguI GCTCTTC 2 cut(s) 50, 771
LmnI GCTCC 2 cut(s) 110, 383
Lsp1109I GCAGC 5 cut(s) 90, 363, 666, 940, 1090
LweI GCATC 1 cut(s) 754
MaeI CTAG 9 cut(s) 318, 366, 522, 867, 974, 1017, 1023, 1032, 1274
MaeII ACGT 1 cut(s) 1375
MaeIII GTNAC 3 cut(s) 473, 510, 674
MalI GATC 6 cut(s) 592, 757, 967, 996, 1187, 1383
MboI GATC 6 cut(s) 590, 755, 965, 994, 1185, 1381
MflI RGATCY 2 cut(s) 590, 755
MhlI GDGCHC 1 cut(s) 1402
MluCI AATT 9 cut(s) 113, 201, 224, 266, 288, 831, 937, 1147, 1240
Mly113I GGCGCC 1 cut(s) 238
MlyI GAGTC 5 cut(s) 116, 386, 869, 879, 1207
MmeI TCCRAC 2 cut(s) 1113, 1226
MnlI CCTC 9 cut(s) 64, 119, 123, 173, 336, 634, 1040, 1043, 1239
MroXI GAANNNNTTC 2 cut(s) 780, 1375
MseI TTAA 6 cut(s) 231, 830, 854, 989, 1011, 1334
MslI CAYNNNNRTG 1 cut(s) 1235
MspA1I CMGCKG 1 cut(s) 40
MspI CCGG 1 cut(s) 242
MspR9I CCNGG 1 cut(s) 242
MvnI CGCG 1 cut(s) 40
MwoI GCNNNNNNNGC 4 cut(s) 906, 915, 1068, 1084
NarI GGCGCC 1 cut(s) 238
NciI CCSGG 1 cut(s) 242
NcoI CCATGG 1 cut(s) 140
NdeII GATC 6 cut(s) 590, 755, 965, 994, 1185, 1381
NheI GCTAGC 1 cut(s) 973
NlaIII CATG 5 cut(s) 144, 169, 482, 1085, 1127
NlaIV GGNNCC 3 cut(s) 6, 239, 650
NmuCI GTSAC 3 cut(s) 473, 510, 674
NspV TTCGAA 1 cut(s) 403
PagI TCATGA 1 cut(s) 165
PciSI GCTCTTC 2 cut(s) 50, 771
PdmI GAANNNNTTC 2 cut(s) 780, 1375
PfeI GAWTC 3 cut(s) 400, 497, 599
PkrI GCNGC 6 cut(s) 80, 353, 656, 955, 1080, 1156
PleI GAGTC 5 cut(s) 116, 386, 869, 878, 1206
PluTI GGCGCC 1 cut(s) 241
PpsI GAGTC 5 cut(s) 116, 386, 869, 878, 1206
Psp1406I AACGTT 1 cut(s) 1375
PspEI GGTNACC 1 cut(s) 473
PspN4I GGNNCC 3 cut(s) 6, 239, 650
PsrI GAACNNNNNNTAC 2 cut(s) 1018, 1050
PsuI RGATCY 2 cut(s) 590, 755
RsaI GTAC 2 cut(s) 568, 1027
RsaNI GTAC 2 cut(s) 567, 1026
RseI CAYNNNNRTG 1 cut(s) 1235
SacII CCGCGG 1 cut(s) 41
SalI GTCGAC 1 cut(s) 872
SapI GCTCTTC 2 cut(s) 50, 771
SaqAI TTAA 6 cut(s) 231, 830, 854, 989, 1011, 1334
SatI GCNGC 6 cut(s) 79, 352, 655, 954, 1079, 1155
Sau3AI GATC 6 cut(s) 590, 755, 965, 994, 1185, 1381
ScaI AGTACT 1 cut(s) 1027
SchI GAGTC 5 cut(s) 116, 386, 869, 879, 1207
ScrFI CCNGG 1 cut(s) 242
SduI GDGCHC 1 cut(s) 1402
SfaNI GCATC 1 cut(s) 754
SfcI CTRYAG 1 cut(s) 594
SfoI GGCGCC 1 cut(s) 239
Sfr303I CCGCGG 1 cut(s) 41
SfuI TTCGAA 1 cut(s) 403
SgrBI CCGCGG 1 cut(s) 41
SmiMI CAYNNNNRTG 1 cut(s) 1235
SmlI CTYRAG 1 cut(s) 127
SmoI CTYRAG 1 cut(s) 127
SpeI ACTAGT 2 cut(s) 1016, 1031
Sse9I AATT 9 cut(s) 113, 201, 224, 266, 288, 831, 937, 1147, 1240
SsiI CCGC 7 cut(s) 30, 38, 40, 652, 1069, 1155, 1316
SspDI GGCGCC 1 cut(s) 237
SspI AATATT 1 cut(s) 1309
SspMI CTAG 9 cut(s) 318, 366, 522, 867, 974, 1017, 1023, 1032, 1274
StyD4I CCNGG 1 cut(s) 240
StyI CCWWGG 4 cut(s) 140, 317, 432, 521
TaaI ACNGT 3 cut(s) 532, 923, 1341
TaiI ACGT 1 cut(s) 1378
TaqI TCGA 7 cut(s) 111, 192, 403, 639, 762, 873, 968
TasI AATT 9 cut(s) 113, 201, 224, 266, 288, 831, 937, 1147, 1240
TatI WGTACW 1 cut(s) 1025
TauI GCSGC 1 cut(s) 1157
TfiI GAWTC 3 cut(s) 400, 497, 599
Tru1I TTAA 6 cut(s) 231, 830, 854, 989, 1011, 1334
Tru9I TTAA 6 cut(s) 231, 830, 854, 989, 1011, 1334
TscAI CASTG 1 cut(s) 577
TseFI GTSAC 3 cut(s) 473, 510, 674
TseI GCWGC 5 cut(s) 78, 351, 654, 953, 1078
Tsp45I GTSAC 3 cut(s) 473, 510, 674
TspDTI ATGAA 6 cut(s) 62, 182, 483, 786, 1134, 1253
TspGWI ACGGA 3 cut(s) 881, 1220, 1247
TspRI CASTG 1 cut(s) 577
XapI RAATTY 5 cut(s) 113, 266, 937, 1147, 1240
XmaJI CCTAGG 2 cut(s) 317, 521
XmiI GTMKAC 1 cut(s) 873
XmnI GAANNNNTTC 2 cut(s) 780, 1375
XspI CTAG 9 cut(s) 318, 366, 522, 867, 974, 1017, 1023, 1032, 1274
ZrmI AGTACT 1 cut(s) 1027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.