Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0002275

Physical Location & Seq

Genomic Coordinates Reverse (-)

8426328 .. 8429901

Gene Structure 3574 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G005220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 945 bp

ATGGCTATGGCGATTCGTTCTGCACTCGCAAGGCTGGTGACTAAATCTATTCACAAGAACAGAAGTGGAATTCTGATGCTTAGGGCTTTTGCGAGCTCAGCCATAGACAAAAACCCCAATCAACAAGACAACGCCGTTTCGGGGTTTCCTGCTGGTCATTACTTCCGTCACCGATTTGATTATGGAAGCCATGAAAGCAAAAAGAAAGAGCTTAAGGTACCTGATTATTATATGGATTTAGGAGGATTAGAGCCTGCCCGTTTCTCCATGGAAGAGTACAACAAGCGAAAGAATGCGCAACTGCAGTTTGTGAGAGTTGTGAAGGCACACAGGCTTCTGCTGTGCACAGGAATTAGCAGCCTATATGTTATAACATTGGAGGCAGTTGATGCCGGAGTGGCTAAACTTTACCAAGCAAAAGTTTCGGAAAATTTCACTGACGGCATGTTTTGGAATGGTTCTGTCTTGTCATTGATGATGGAAAACTTTTTTCCTCAGAACGACCATGAAGTCAAAGCGGAGAATCAAACTCCGATGTGTAAAAAGAATGGACTTAATTGTTCTAATAACTCATGTATCAAACTATGTCGTATTGAACCTGCATATGACTTATCAAATAATGACCAGATGCTAAGATTTGGACATTGGGCCGTGAAAATGTTCAACGAGGAAAAGAATGCCCAGCTGCGATTCATTAGAGCAGTAAAGGGATTTCGCAGTTGTTTTGATTACTTTCTAACTTTGGAGGCAGTCGATGCTCATGCGGTTAGAACTTACCAAGCAGTAGTCAAAGAAAAGCGCATGGATCCACATGAACCCTTTAAGGTCCTATTATTTGGTCTTGTTGCTGATAACGGCAATGGGAATTTGCTAAATCTAATTGATAACAGAAGGAGTGGACCGTATGAAGAAAATATGTTGCTTGATACAAGTGATAAACGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002020 GO:0003674 GO:0004857 GO:0004866 GO:0004869 GO:0005488 GO:0005515 GO:0008150 GO:0009892 GO:0010466 GO:0010605 GO:0010951 GO:0019222 GO:0019899 GO:0030162 GO:0030234 GO:0030414 GO:0031323 GO:0031324 GO:0032268 GO:0032269 GO:0043086 GO:0044092 GO:0045861 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0065007 GO:0065009 GO:0080090 GO:0098772 GO:2000116 GO:2000117

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cystatin SQAPI

Protein Analysis

314

Amino Acids

35.84

Weight (kDa)

9.09

Isoelectric Point (pI)

38.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SQAPI	PF16845	86 - 147	3.9e-06	Aspartic acid proteinase inhibitor
SQAPI	PF16845	208 - 269	4.4e-06	Aspartic acid proteinase inhibitor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002275)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g36030 FvH4_6g36030 FvH4_6g36030
malus_domestica	MD17G1149700.v1.1
pyrus_communis	pycom17g14320
rosa_chinensis	RchiOBHm_Chr2g0143241 RchiOBHm_Chr2g0143301 RchiOBHm_Chr6g0253161 RchiOBHm_Chr7g0231321
rosa_laevigata	RLG00000012687 RLG00000020018 RLG00000020022 RLG00000020025 RLG00000036534 RLG00000036535
rosa_multiflora	Rmu_co8311305.1_g000001 Rmu_sc0003507.1_g000005 Rmu_sc0003898.1_g000007 Rmu_sc0003950.1_g000001
rosa_roxburghii	Rroxscaffold_1G00006450 Rroxscaffold_2G00101890 Rroxscaffold_4G00318720 Rroxscaffold_7G00212100
rosa_rugosa	Rorug02G0379700 Rorug02G0379800
rosa_samantha	Rh1DG126100 Rh2AG432600 Rh2AG432900 Rh2BG441200 Rh2CG418400 Rh2CG418700 Rh2DG451300 Rh5DG528000 Rh6AG057400 Rh6BG052000 Rh6CG049900 Rh6DG046100 Rh7AG420100 Rh7CG439600
rosa_wichuraiana	Rw1G009770 Rw2G035320 Rw5G045830 Rw6G005220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	371
AasI	GACNNNNNNGTC	1 cut(s)	509
Acc16I	TGCGCA	1 cut(s)	297
Acc36I	ACCTGC	1 cut(s)	607
Acc65I	GGTACC	1 cut(s)	217
AccB1I	GGYRCC	1 cut(s)	217
AciI	CCGC	2 cut(s)	518, 764
AclWI	GGATC	2 cut(s)	800, 813
AcsI	RAATTY	3 cut(s)	69, 430, 865
AfaI	GTAC	2 cut(s)	219, 278
AfiI	CCNNNNNNNGG	1 cut(s)	141
AflII	CTTAAG	1 cut(s)	212
AgsI	TTSAA	2 cut(s)	596, 664
AluBI	AGCT	3 cut(s)	96, 211, 685
AluI	AGCT	3 cut(s)	96, 211, 685
Alw21I	GWGCWC	2 cut(s)	98, 347
Alw44I	GTGCAC	1 cut(s)	343
AlwI	GGATC	2 cut(s)	800, 813
AoxI	GGCC	1 cut(s)	648
ApaLI	GTGCAC	1 cut(s)	343
ApeKI	GCWGC	2 cut(s)	357, 685
ApoI	RAATTY	3 cut(s)	69, 430, 865
Asp700I	GAANNNNTTC	1 cut(s)	659
Asp718I	GGTACC	1 cut(s)	217
AspLEI	GCGC	2 cut(s)	298, 801
AspS9I	GGNCC	3 cut(s)	648, 826, 899
AsuHPI	GGTGA	2 cut(s)	49, 161
AvaII	GGWCC	2 cut(s)	826, 899
BaeGI	GKGCMC	1 cut(s)	347
BaeI	ACNNNNGTAYC	2 cut(s)	559, 592
BamHI	GGATCC	1 cut(s)	805
BanI	GGYRCC	1 cut(s)	217
BanII	GRGCYC	1 cut(s)	98
Bbv12I	GWGCWC	2 cut(s)	98, 347
BbvI	GCAGC	2 cut(s)	369, 672
BccI	CCATC	1 cut(s)	472
BceAI	ACGGC	4 cut(s)	119, 457, 635, 871
BcgI	CGANNNNNNTGC	4 cut(s)	405, 439, 743, 777
BfmI	CTRYAG	1 cut(s)	302
BfrI	CTTAAG	1 cut(s)	212
BfuAI	ACCTGC	1 cut(s)	607
BglI	GCCNNNNNGGC	1 cut(s)	398
BisI	GCNGC	2 cut(s)	358, 686
BlpI	GCTNAGC	1 cut(s)	97
BlsI	GCNGC	2 cut(s)	359, 687
Bme18I	GGWCC	2 cut(s)	826, 899
BmgT120I	GGNCC	3 cut(s)	648, 826, 899
BmiI	GGNNCC	2 cut(s)	219, 807
BmsI	GCATC	4 cut(s)	66, 379, 618, 745
Bpu10I	CCTNAGC	1 cut(s)	80
Bpu1102I	GCTNAGC	1 cut(s)	97
BsaJI	CCNNGG	1 cut(s)	267
Bsc4I	CCNNNNNNNGG	1 cut(s)	141
Bse3DI	GCAATG	1 cut(s)	865
BseDI	CCNNGG	1 cut(s)	267
BseLI	CCNNNNNNNGG	1 cut(s)	141
BseMI	GCAATG	1 cut(s)	865
BseMII	CTCAG	2 cut(s)	111, 509
BseSI	GKGCMC	1 cut(s)	347
BseXI	GCAGC	2 cut(s)	369, 672
BseYI	CCCAGC	1 cut(s)	681
BsgI	GTGCAG	1 cut(s)	6
BshFI	GGCC	1 cut(s)	650
BshNI	GGYRCC	1 cut(s)	217
BsiHKAI	GWGCWC	2 cut(s)	98, 347
BsiSI	CCGG	1 cut(s)	393
BslI	CCNNNNNNNGG	1 cut(s)	141
BsmI	GAATGC	2 cut(s)	298, 682
BsnI	GGCC	1 cut(s)	650
Bsp1286I	GDGCHC	2 cut(s)	98, 347
Bsp143I	GATC	1 cut(s)	805
Bsp1720I	GCTNAGC	1 cut(s)	97
Bsp19I	CCATGG	1 cut(s)	267
BspACI	CCGC	2 cut(s)	518, 764
BspANI	GGCC	1 cut(s)	650
BspCNI	CTCAG	2 cut(s)	110, 508
BspLI	GGNNCC	2 cut(s)	219, 807
BspMAI	CTGCAG	1 cut(s)	306
BspMI	ACCTGC	1 cut(s)	607
BspPI	GGATC	2 cut(s)	800, 813
BspT107I	GGYRCC	1 cut(s)	217
BspTI	CTTAAG	1 cut(s)	212
BsrDI	GCAATG	1 cut(s)	865
BssECI	CCNNGG	1 cut(s)	267
BssMI	GATC	1 cut(s)	805
BssT1I	CCWWGG	1 cut(s)	267
Bst4CI	ACNGT	2 cut(s)	903, 942
Bst6I	CTCTTC	1 cut(s)	267
BstAFI	CTTAAG	1 cut(s)	212
BstAPI	GCANNNNNTGC	2 cut(s)	389, 755
BstC8I	GCNNGC	2 cut(s)	94, 255
BstDEI	CTNAG	4 cut(s)	80, 97, 495, 632
BstDSI	CCRYGG	1 cut(s)	267
BstHHI	GCGC	2 cut(s)	298, 801
BstKTI	GATC	1 cut(s)	808
BstMBI	GATC	1 cut(s)	805
BstMWI	GCNNNNNNNGC	5 cut(s)	98, 195, 389, 398, 755
BstNSI	RCATGY	1 cut(s)	448
BstSFI	CTRYAG	1 cut(s)	302
BstSLI	GKGCMC	1 cut(s)	347
BstV1I	GCAGC	2 cut(s)	369, 672
BstX2I	RGATCY	1 cut(s)	805
BstYI	RGATCY	1 cut(s)	805
BsuRI	GGCC	1 cut(s)	650
BtgI	CCRYGG	1 cut(s)	267
BtsIMutI	CAGTG	1 cut(s)	435
BveI	ACCTGC	1 cut(s)	607
Cac8I	GCNNGC	2 cut(s)	94, 255
CfoI	GCGC	2 cut(s)	298, 801
Cfr13I	GGNCC	3 cut(s)	648, 826, 899
Csp6I	GTAC	2 cut(s)	218, 277
CviAII	CATG	8 cut(s)	191, 268, 445, 506, 573, 761, 802, 812
CviQI	GTAC	2 cut(s)	218, 277
DdeI	CTNAG	4 cut(s)	80, 97, 495, 632
DpnI	GATC	1 cut(s)	807
DpnII	GATC	1 cut(s)	805
DrdI	GACNNNNNNGTC	1 cut(s)	509
DseDI	GACNNNNNNGTC	1 cut(s)	509
Eam1104I	CTCTTC	1 cut(s)	267
EarI	CTCTTC	1 cut(s)	267
Ecl136II	GAGCTC	1 cut(s)	96
Eco130I	CCWWGG	1 cut(s)	267
Eco24I	GRGCYC	1 cut(s)	98
Eco47I	GGWCC	2 cut(s)	826, 899
Eco53kI	GAGCTC	1 cut(s)	96
EcoICRI	GAGCTC	1 cut(s)	96
EcoO109I	RGGNCCY	1 cut(s)	826
EcoRI	GAATTC	1 cut(s)	69
EcoT14I	CCWWGG	1 cut(s)	267
EcoT38I	GRGCYC	1 cut(s)	98
ErhI	CCWWGG	1 cut(s)	267
FaeI	CATG	8 cut(s)	194, 271, 448, 509, 576, 764, 805, 815
FatI	CATG	8 cut(s)	190, 267, 444, 505, 572, 760, 801, 811
FauNDI	CATATG	1 cut(s)	604
Fnu4HI	GCNGC	2 cut(s)	358, 686
FriOI	GRGCYC	1 cut(s)	98
Fsp4HI	GCNGC	2 cut(s)	358, 686
FspI	TGCGCA	1 cut(s)	297
GlaI	GCGC	2 cut(s)	297, 800
GluI	GCNGC	2 cut(s)	358, 686
GsaI	CCCAGC	1 cut(s)	685
HaeIII	GGCC	1 cut(s)	650
HapII	CCGG	1 cut(s)	393
HhaI	GCGC	2 cut(s)	298, 801
Hin1II	CATG	8 cut(s)	194, 271, 448, 509, 576, 764, 805, 815
Hin6I	GCGC	2 cut(s)	296, 799
HinP1I	GCGC	2 cut(s)	296, 799
HinfI	GANTC	3 cut(s)	13, 523, 690
HpaII	CCGG	1 cut(s)	393
HphI	GGTGA	2 cut(s)	49, 161
Hpy166II	GTNNAC	2 cut(s)	345, 899
Hpy188I	TCNGA	4 cut(s)	75, 427, 498, 534
Hpy8I	GTNNAC	2 cut(s)	345, 899
HpyAV	CCTTC	2 cut(s)	316, 885
HpyCH4III	ACNGT	2 cut(s)	903, 942
HpyCH4V	TGCA	4 cut(s)	23, 304, 345, 602
HpyF10VI	GCNNNNNNNGC	5 cut(s)	98, 195, 389, 398, 755
HpyF3I	CTNAG	4 cut(s)	80, 97, 495, 632
Hsp92II	CATG	8 cut(s)	194, 271, 448, 509, 576, 764, 805, 815
HspAI	GCGC	2 cut(s)	296, 799
KpnI	GGTACC	1 cut(s)	221
Kzo9I	GATC	1 cut(s)	805
Lsp1109I	GCAGC	2 cut(s)	369, 672
LweI	GCATC	4 cut(s)	66, 379, 618, 745
MaeIII	GTNAC	2 cut(s)	37, 167
MalI	GATC	1 cut(s)	807
MboI	GATC	1 cut(s)	805
MboII	GAAGA	2 cut(s)	284, 920
MflI	RGATCY	1 cut(s)	805
MhlI	GDGCHC	2 cut(s)	98, 347
MluCI	AATT	6 cut(s)	69, 351, 430, 556, 865, 879
MnlI	CCTC	5 cut(s)	236, 373, 504, 661, 739
MroXI	GAANNNNTTC	1 cut(s)	659
MseI	TTAA	3 cut(s)	213, 555, 822
MspA1I	CMGCKG	1 cut(s)	685
MspCI	CTTAAG	1 cut(s)	212
MspI	CCGG	1 cut(s)	393
Mva1269I	GAATGC	2 cut(s)	298, 682
MwoI	GCNNNNNNNGC	5 cut(s)	98, 195, 389, 398, 755
NcoI	CCATGG	1 cut(s)	267
NdeI	CATATG	1 cut(s)	604
NdeII	GATC	1 cut(s)	805
NlaIII	CATG	8 cut(s)	194, 271, 448, 509, 576, 764, 805, 815
NlaIV	GGNNCC	2 cut(s)	219, 807
NmuCI	GTSAC	2 cut(s)	37, 167
NsbI	TGCGCA	1 cut(s)	297
NspI	RCATGY	1 cut(s)	448
PctI	GAATGC	2 cut(s)	298, 682
PdmI	GAANNNNTTC	1 cut(s)	659
PfeI	GAWTC	3 cut(s)	13, 523, 690
PkrI	GCNGC	2 cut(s)	359, 687
PpuMI	RGGWCCY	1 cut(s)	826
PsiI	TTATAA	1 cut(s)	371
Psp124BI	GAGCTC	1 cut(s)	98
Psp5II	RGGWCCY	1 cut(s)	826
PspFI	CCCAGC	1 cut(s)	681
PspN4I	GGNNCC	2 cut(s)	219, 807
PspPI	GGNCC	3 cut(s)	648, 826, 899
PspPPI	RGGWCCY	1 cut(s)	826
PstI	CTGCAG	1 cut(s)	306
PsuI	RGATCY	1 cut(s)	805
PvuII	CAGCTG	1 cut(s)	685
RsaI	GTAC	2 cut(s)	219, 278
RsaNI	GTAC	2 cut(s)	218, 277
SacI	GAGCTC	1 cut(s)	98
SaqAI	TTAA	3 cut(s)	213, 555, 822
SatI	GCNGC	2 cut(s)	358, 686
Sau3AI	GATC	1 cut(s)	805
Sau96I	GGNCC	3 cut(s)	648, 826, 899
SduI	GDGCHC	2 cut(s)	98, 347
SetI	ASST	7 cut(s)	98, 213, 219, 223, 601, 687, 828
SfaNI	GCATC	4 cut(s)	66, 379, 618, 745
SfcI	CTRYAG	1 cut(s)	302
SinI	GGWCC	2 cut(s)	826, 899
SmlI	CTYRAG	1 cut(s)	212
SmoI	CTYRAG	1 cut(s)	212
Sse9I	AATT	6 cut(s)	69, 351, 430, 556, 865, 879
SsiI	CCGC	2 cut(s)	518, 764
SstI	GAGCTC	1 cut(s)	98
StyI	CCWWGG	1 cut(s)	267
TaaI	ACNGT	2 cut(s)	903, 942
TaqI	TCGA	1 cut(s)	753
TasI	AATT	6 cut(s)	69, 351, 430, 556, 865, 879
TatI	WGTACW	1 cut(s)	276
TfiI	GAWTC	3 cut(s)	13, 523, 690
Tru1I	TTAA	3 cut(s)	213, 555, 822
Tru9I	TTAA	3 cut(s)	213, 555, 822
TscAI	CASTG	1 cut(s)	442
TseFI	GTSAC	2 cut(s)	37, 167
TseI	GCWGC	2 cut(s)	357, 685
Tsp45I	GTSAC	2 cut(s)	37, 167
TspDTI	ATGAA	5 cut(s)	207, 522, 682, 828, 921
TspGWI	ACGGA	1 cut(s)	155
TspRI	CASTG	1 cut(s)	442
Vha464I	CTTAAG	1 cut(s)	212
VneI	GTGCAC	1 cut(s)	343
VpaK11BI	GGWCC	2 cut(s)	826, 899
XapI	RAATTY	3 cut(s)	69, 430, 865
XceI	RCATGY	1 cut(s)	448
XmnI	GAANNNNTTC	1 cut(s)	659

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.