subtilisin-like protease
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 1573, 2137 |
| AccI | GTMKAC | 3 cut(s) | 1121, 2082, 2091 |
| AccII | CGCG | 2 cut(s) | 618, 745 |
| AclI | AACGTT | 1 cut(s) | 2070 |
| AclWI | GGATC | 6 cut(s) | 116, 792, 1037, 1760, 1811, 1859 |
| AcoI | YGGCCR | 1 cut(s) | 437 |
| AcsI | RAATTY | 1 cut(s) | 518 |
| AcuI | CTGAAG | 1 cut(s) | 303 |
| AdeI | CACNNNGTG | 1 cut(s) | 746 |
| AfaI | GTAC | 6 cut(s) | 63, 206, 563, 1231, 1422, 1928 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 1234, 1568, 2117 |
| AgeI | ACCGGT | 1 cut(s) | 1059 |
| AgsI | TTSAA | 4 cut(s) | 55, 826, 1620, 2126 |
| AhlI | ACTAGT | 1 cut(s) | 2149 |
| AjnI | CCWGG | 1 cut(s) | 1348 |
| AleI | CACNNNNGTG | 2 cut(s) | 431, 852 |
| Alw21I | GWGCWC | 2 cut(s) | 751, 1410 |
| Alw26I | GTCTC | 7 cut(s) | 630, 1178, 1722, 1877, 1890, 2138, 2182 |
| AlwI | GGATC | 6 cut(s) | 116, 792, 1037, 1760, 1811, 1859 |
| AlwNI | CAGNNNCTG | 4 cut(s) | 446, 1043, 1117, 2299 |
| Ama87I | CYCGRG | 1 cut(s) | 1409 |
| AoxI | GGCC | 3 cut(s) | 437, 801, 991 |
| ApeKI | GCWGC | 9 cut(s) | 112, 236, 1043, 1295, 1313, 1595, 1661, 1724, 1859 |
| ApoI | RAATTY | 1 cut(s) | 518 |
| AsiGI | ACCGGT | 1 cut(s) | 1059 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 288 |
| AspLEI | GCGC | 4 cut(s) | 740, 797, 884, 1209 |
| AspS9I | GGNCC | 7 cut(s) | 473, 992, 1057, 1062, 1529, 1889, 2188 |
| AsuC2I | CCSGG | 3 cut(s) | 1578, 1869, 1924 |
| AsuHPI | GGTGA | 8 cut(s) | 116, 140, 410, 1135, 1168, 1440, 2011, 2044 |
| AvaI | CYCGRG | 1 cut(s) | 1409 |
| AvaII | GGWCC | 6 cut(s) | 473, 1057, 1062, 1529, 1889, 2188 |
| BaeGI | GKGCMC | 1 cut(s) | 1576 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1269, 1302 |
| BalI | TGGCCA | 1 cut(s) | 439 |
| BanI | GGYRCC | 2 cut(s) | 1573, 2137 |
| BanII | GRGCYC | 1 cut(s) | 1410 |
| Bbv12I | GWGCWC | 2 cut(s) | 751, 1410 |
| BbvI | GCAGC | 9 cut(s) | 124, 223, 1030, 1282, 1325, 1582, 1673, 1711, 1846 |
| BccI | CCATC | 3 cut(s) | 845, 1651, 2030 |
| BceAI | ACGGC | 2 cut(s) | 589, 1996 |
| BciT130I | CCWGG | 1 cut(s) | 1350 |
| BciVI | GTATCC | 1 cut(s) | 909 |
| BclI | TGATCA | 1 cut(s) | 1332 |
| BcnI | CCSGG | 3 cut(s) | 1578, 1869, 1924 |
| BcoDI | GTCTC | 7 cut(s) | 630, 1178, 1722, 1877, 1890, 2138, 2182 |
| BcuI | ACTAGT | 1 cut(s) | 2149 |
| BfaI | CTAG | 6 cut(s) | 69, 633, 791, 1443, 1473, 2150 |
| BfmI | CTRYAG | 2 cut(s) | 1749, 1860 |
| BfoI | RGCGCY | 2 cut(s) | 885, 1210 |
| BfuI | GTATCC | 1 cut(s) | 909 |
| BmcAI | AGTACT | 1 cut(s) | 563 |
| Bme1390I | CCNGG | 4 cut(s) | 1350, 1578, 1869, 1924 |
| Bme18I | GGWCC | 6 cut(s) | 473, 1057, 1062, 1529, 1889, 2188 |
| BmeT110I | CYCGRG | 1 cut(s) | 1409 |
| BmgT120I | GGNCC | 7 cut(s) | 473, 992, 1057, 1062, 1529, 1889, 2188 |
| BmiI | GGNNCC | 5 cut(s) | 556, 1091, 1575, 1890, 2139 |
| BmrFI | CCNGG | 4 cut(s) | 1350, 1578, 1869, 1924 |
| BmrI | ACTGGG | 1 cut(s) | 1115 |
| BmsI | GCATC | 3 cut(s) | 175, 969, 1714 |
| BmuI | ACTGGG | 1 cut(s) | 1115 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1887 |
| BpmI | CTGGAG | 3 cut(s) | 738, 954, 1650 |
| BpuEI | CTTGAG | 1 cut(s) | 748 |
| BpuMI | CCSGG | 3 cut(s) | 1578, 1869, 1924 |
| Bsa29I | ATCGAT | 1 cut(s) | 1655 |
| BsaAI | YACGTR | 1 cut(s) | 1306 |
| BsaBI | GATNNNNATC | 1 cut(s) | 505 |
| BsaI | GGTCTC | 3 cut(s) | 1178, 1722, 1877 |
| BsaJI | CCNNGG | 1 cut(s) | 1025 |
| BsaWI | WCCGGW | 2 cut(s) | 1059, 2140 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 1421, 1451, 1827, 1857, 1901, 1931 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 1234, 1568, 2117 |
| Bse118I | RCCGGY | 2 cut(s) | 1059, 1316 |
| Bse1I | ACTGG | 5 cut(s) | 937, 1110, 1146, 1667, 2224 |
| Bse3DI | GCAATG | 1 cut(s) | 837 |
| Bse8I | GATNNNNATC | 1 cut(s) | 505 |
| BseBI | CCWGG | 1 cut(s) | 1350 |
| BseCI | ATCGAT | 1 cut(s) | 1655 |
| BseDI | CCNNGG | 1 cut(s) | 1025 |
| BseGI | GGATG | 5 cut(s) | 190, 506, 841, 1705, 2022 |
| BseJI | GATNNNNATC | 1 cut(s) | 505 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 1234, 1568, 2117 |
| BseMI | GCAATG | 1 cut(s) | 837 |
| BseMII | CTCAG | 5 cut(s) | 117, 899, 1016, 1842, 1961 |
| BseNI | ACTGG | 5 cut(s) | 937, 1110, 1146, 1667, 2224 |
| BseSI | GKGCMC | 1 cut(s) | 1576 |
| BseXI | GCAGC | 9 cut(s) | 124, 223, 1030, 1282, 1325, 1582, 1673, 1711, 1846 |
| BseYI | CCCAGC | 2 cut(s) | 994, 1546 |
| BsgI | GTGCAG | 1 cut(s) | 996 |
| Bsh1236I | CGCG | 2 cut(s) | 618, 745 |
| BshFI | GGCC | 3 cut(s) | 439, 803, 993 |
| BshNI | GGYRCC | 2 cut(s) | 1573, 2137 |
| BshTI | ACCGGT | 1 cut(s) | 1059 |
| BshVI | ATCGAT | 1 cut(s) | 1655 |
| BsiHKAI | GWGCWC | 2 cut(s) | 751, 1410 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1409 |
| BsiSI | CCGG | 6 cut(s) | 1060, 1317, 1577, 1869, 1923, 2141 |
| BslFI | GGGAC | 2 cut(s) | 1426, 1513 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 1234, 1568, 2117 |
| BsmAI | GTCTC | 7 cut(s) | 630, 1178, 1722, 1877, 1890, 2138, 2182 |
| BsmFI | GGGAC | 2 cut(s) | 1426, 1513 |
| BsmI | GAATGC | 1 cut(s) | 2121 |
| BsnI | GGCC | 3 cut(s) | 439, 803, 993 |
| Bso31I | GGTCTC | 3 cut(s) | 1178, 1722, 1877 |
| BsoBI | CYCGRG | 1 cut(s) | 1409 |
| Bsp1286I | GDGCHC | 3 cut(s) | 751, 1410, 1576 |
| Bsp1407I | TGTACA | 1 cut(s) | 204 |
| BspANI | GGCC | 3 cut(s) | 439, 803, 993 |
| BspCNI | CTCAG | 5 cut(s) | 118, 898, 1015, 1843, 1960 |
| BspDI | ATCGAT | 1 cut(s) | 1655 |
| BspFNI | CGCG | 2 cut(s) | 618, 745 |
| BspHI | TCATGA | 1 cut(s) | 190 |
| BspLI | GGNNCC | 5 cut(s) | 556, 1091, 1575, 1890, 2139 |
| BspMAI | CTGCAG | 1 cut(s) | 1864 |
| BspPI | GGATC | 6 cut(s) | 116, 792, 1037, 1760, 1811, 1859 |
| BspT107I | GGYRCC | 2 cut(s) | 1573, 2137 |
| BspTNI | GGTCTC | 3 cut(s) | 1178, 1722, 1877 |
| BsrDI | GCAATG | 1 cut(s) | 837 |
| BsrFI | RCCGGY | 2 cut(s) | 1059, 1316 |
| BsrGI | TGTACA | 1 cut(s) | 204 |
| BsrI | ACTGG | 5 cut(s) | 937, 1110, 1146, 1667, 2224 |
| BssAI | RCCGGY | 2 cut(s) | 1059, 1316 |
| BssECI | CCNNGG | 1 cut(s) | 1025 |
| BssNAI | GTATAC | 1 cut(s) | 1122 |
| BssT1I | CCWWGG | 1 cut(s) | 1025 |
| Bst1107I | GTATAC | 1 cut(s) | 1122 |
| Bst2UI | CCWGG | 1 cut(s) | 1350 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 746, 2299 |
| BstAUI | TGTACA | 1 cut(s) | 204 |
| BstBAI | YACGTR | 1 cut(s) | 1306 |
| BstC8I | GCNNGC | 2 cut(s) | 747, 751 |
| BstDEI | CTNAG | 7 cut(s) | 35, 126, 346, 885, 1002, 1851, 1947 |
| BstEII | GGTNACC | 1 cut(s) | 398 |
| BstF5I | GGATG | 5 cut(s) | 190, 506, 841, 1705, 2022 |
| BstFNI | CGCG | 2 cut(s) | 618, 745 |
| BstH2I | RGCGCY | 2 cut(s) | 885, 1210 |
| BstHHI | GCGC | 4 cut(s) | 740, 797, 884, 1209 |
| BstMAI | GTCTC | 7 cut(s) | 630, 1178, 1722, 1877, 1890, 2138, 2182 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 671, 746, 755, 935, 944, 2299 |
| BstNI | CCWGG | 1 cut(s) | 1350 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1887 |
| BstPI | GGTNACC | 1 cut(s) | 398 |
| BstSCI | CCNGG | 4 cut(s) | 1348, 1576, 1867, 1922 |
| BstSFI | CTRYAG | 2 cut(s) | 1749, 1860 |
| BstSLI | GKGCMC | 1 cut(s) | 1576 |
| BstUI | CGCG | 2 cut(s) | 618, 745 |
| BstV1I | GCAGC | 9 cut(s) | 124, 223, 1030, 1282, 1325, 1582, 1673, 1711, 1846 |
| BstX2I | RGATCY | 2 cut(s) | 1765, 1864 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 489 |
| BstYI | RGATCY | 2 cut(s) | 1765, 1864 |
| BstZ17I | GTATAC | 1 cut(s) | 1122 |
| Bsu15I | ATCGAT | 1 cut(s) | 1655 |
| BsuI | GTATCC | 1 cut(s) | 909 |
| BsuRI | GGCC | 3 cut(s) | 439, 803, 993 |
| BsuTUI | ATCGAT | 1 cut(s) | 1655 |
| BtgZI | GCGATG | 1 cut(s) | 1321 |
| BtsCI | GGATG | 5 cut(s) | 190, 506, 841, 1705, 2022 |
| BtsI | GCAGTG | 2 cut(s) | 804, 1017 |
| BtsIMutI | CAGTG | 5 cut(s) | 804, 1017, 1044, 1115, 2217 |
| Cac8I | GCNNGC | 2 cut(s) | 747, 751 |
| CaiI | CAGNNNCTG | 4 cut(s) | 446, 1043, 1117, 2299 |
| CciI | TCATGA | 1 cut(s) | 190 |
| CfoI | GCGC | 4 cut(s) | 740, 797, 884, 1209 |
| Cfr10I | RCCGGY | 2 cut(s) | 1059, 1316 |
| Cfr13I | GGNCC | 7 cut(s) | 473, 992, 1057, 1062, 1529, 1889, 2188 |
| ClaI | ATCGAT | 1 cut(s) | 1655 |
| CpoI | CGGWCCG | 1 cut(s) | 2188 |
| Csp6I | GTAC | 6 cut(s) | 62, 205, 562, 1230, 1421, 1927 |
| CspAI | ACCGGT | 1 cut(s) | 1059 |
| CspI | CGGWCCG | 1 cut(s) | 2188 |
| CviQI | GTAC | 6 cut(s) | 62, 205, 562, 1230, 1421, 1927 |
| DdeI | CTNAG | 7 cut(s) | 35, 126, 346, 885, 1002, 1851, 1947 |
| DraIII | CACNNNGTG | 1 cut(s) | 746 |
| EaeI | YGGCCR | 1 cut(s) | 437 |
| EciI | GGCGGA | 2 cut(s) | 317, 1909 |
| Ecl136II | GAGCTC | 1 cut(s) | 1408 |
| Eco130I | CCWWGG | 1 cut(s) | 1025 |
| Eco24I | GRGCYC | 1 cut(s) | 1410 |
| Eco31I | GGTCTC | 3 cut(s) | 1178, 1722, 1877 |
| Eco32I | GATATC | 1 cut(s) | 820 |
| Eco47I | GGWCC | 6 cut(s) | 473, 1057, 1062, 1529, 1889, 2188 |
| Eco53kI | GAGCTC | 1 cut(s) | 1408 |
| Eco57I | CTGAAG | 1 cut(s) | 303 |
| Eco88I | CYCGRG | 1 cut(s) | 1409 |
| Eco91I | GGTNACC | 1 cut(s) | 398 |
| EcoICRI | GAGCTC | 1 cut(s) | 1408 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1529 |
| EcoO65I | GGTNACC | 1 cut(s) | 398 |
| EcoRII | CCWGG | 1 cut(s) | 1348 |
| EcoRV | GATATC | 1 cut(s) | 820 |
| EcoT14I | CCWWGG | 1 cut(s) | 1025 |
| EcoT22I | ATGCAT | 1 cut(s) | 2262 |
| EcoT38I | GRGCYC | 1 cut(s) | 1410 |
| ErhI | CCWWGG | 1 cut(s) | 1025 |
| FaqI | GGGAC | 2 cut(s) | 1426, 1513 |
| FauI | CCCGC | 2 cut(s) | 1639, 1669 |
| FbaI | TGATCA | 1 cut(s) | 1332 |
| FblI | GTMKAC | 3 cut(s) | 1121, 2082, 2091 |
| FokI | GGATG | 5 cut(s) | 197, 513, 848, 1692, 2009 |
| FriOI | GRGCYC | 1 cut(s) | 1410 |
| FspBI | CTAG | 6 cut(s) | 69, 633, 791, 1443, 1473, 2150 |
| GlaI | GCGC | 4 cut(s) | 739, 796, 883, 1208 |
| GsaI | CCCAGC | 2 cut(s) | 998, 1550 |
| GsuI | CTGGAG | 3 cut(s) | 738, 954, 1650 |
| HaeII | RGCGCY | 2 cut(s) | 885, 1210 |
| HaeIII | GGCC | 3 cut(s) | 439, 803, 993 |
| HapII | CCGG | 6 cut(s) | 1060, 1317, 1577, 1869, 1923, 2141 |
| HhaI | GCGC | 4 cut(s) | 740, 797, 884, 1209 |
| Hin6I | GCGC | 4 cut(s) | 738, 795, 882, 1207 |
| HinP1I | GCGC | 4 cut(s) | 738, 795, 882, 1207 |
| HincII | GTYRAC | 1 cut(s) | 2083 |
| HindII | GTYRAC | 1 cut(s) | 2083 |
| HindIII | AAGCTT | 2 cut(s) | 242, 1698 |
| HpaII | CCGG | 6 cut(s) | 1060, 1317, 1577, 1869, 1923, 2141 |
| HphI | GGTGA | 8 cut(s) | 116, 140, 410, 1135, 1168, 1440, 2011, 2044 |
| Hpy166II | GTNNAC | 7 cut(s) | 205, 1122, 1156, 1176, 1846, 2083, 2092 |
| Hpy188I | TCNGA | 6 cut(s) | 139, 448, 817, 888, 1005, 1775 |
| Hpy8I | GTNNAC | 7 cut(s) | 205, 1122, 1156, 1176, 1846, 2083, 2092 |
| HpyAV | CCTTC | 5 cut(s) | 587, 1303, 1479, 1787, 2191 |
| HpyCH4IV | ACGT | 2 cut(s) | 1305, 2070 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 671, 746, 755, 935, 944, 2299 |
| HpyF3I | CTNAG | 7 cut(s) | 35, 126, 346, 885, 1002, 1851, 1947 |
| HpySE526I | ACGT | 2 cut(s) | 1305, 2070 |
| HspAI | GCGC | 4 cut(s) | 738, 795, 882, 1207 |
| Ksp22I | TGATCA | 1 cut(s) | 1332 |
| LmnI | GCTCC | 6 cut(s) | 554, 935, 1027, 1442, 1669, 1922 |
| Lsp1109I | GCAGC | 9 cut(s) | 124, 223, 1030, 1282, 1325, 1582, 1673, 1711, 1846 |
| LweI | GCATC | 3 cut(s) | 175, 969, 1714 |
| MaeI | CTAG | 6 cut(s) | 69, 633, 791, 1443, 1473, 2150 |
| MaeII | ACGT | 2 cut(s) | 1305, 2070 |
| MboII | GAAGA | 2 cut(s) | 804, 1471 |
| MflI | RGATCY | 2 cut(s) | 1765, 1864 |
| MhlI | GDGCHC | 3 cut(s) | 751, 1410, 1576 |
| MlsI | TGGCCA | 1 cut(s) | 439 |
| MluCI | AATT | 6 cut(s) | 7, 518, 532, 1615, 1711, 2005 |
| MluNI | TGGCCA | 1 cut(s) | 439 |
| MlyI | GAGTC | 5 cut(s) | 260, 452, 705, 1928, 2078 |
| MmeI | TCCRAC | 3 cut(s) | 451, 1465, 2053 |
| Mox20I | TGGCCA | 1 cut(s) | 439 |
| Mph1103I | ATGCAT | 1 cut(s) | 2262 |
| MroXI | GAANNNNTTC | 1 cut(s) | 288 |
| MscI | TGGCCA | 1 cut(s) | 439 |
| MseI | TTAA | 8 cut(s) | 456, 600, 1013, 1212, 1557, 1695, 2034, 2157 |
| MslI | CAYNNNNRTG | 4 cut(s) | 431, 852, 1037, 2126 |
| Msp20I | TGGCCA | 1 cut(s) | 439 |
| MspA1I | CMGCKG | 1 cut(s) | 998 |
| MspI | CCGG | 6 cut(s) | 1060, 1317, 1577, 1869, 1923, 2141 |
| MspR9I | CCNGG | 4 cut(s) | 1350, 1578, 1869, 1924 |
| Mva1269I | GAATGC | 1 cut(s) | 2121 |
| MvaI | CCWGG | 1 cut(s) | 1350 |
| MvnI | CGCG | 2 cut(s) | 618, 745 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 671, 746, 755, 935, 944, 2299 |
| NciI | CCSGG | 3 cut(s) | 1578, 1869, 1924 |
| NlaIV | GGNNCC | 5 cut(s) | 556, 1091, 1575, 1890, 2139 |
| NmeAIII | GCCGAG | 1 cut(s) | 858 |
| NmuCI | GTSAC | 7 cut(s) | 162, 252, 398, 1744, 2044, 2062, 2266 |
| NsiI | ATGCAT | 1 cut(s) | 2262 |
| OliI | CACNNNNGTG | 2 cut(s) | 431, 852 |
| PagI | TCATGA | 1 cut(s) | 190 |
| PctI | GAATGC | 1 cut(s) | 2121 |
| PdmI | GAANNNNTTC | 1 cut(s) | 288 |
| PflFI | GACNNNGTC | 2 cut(s) | 2042, 2087 |
| PinAI | ACCGGT | 1 cut(s) | 1059 |
| PleI | GAGTC | 5 cut(s) | 259, 451, 705, 1928, 2078 |
| PpsI | GAGTC | 5 cut(s) | 259, 451, 705, 1928, 2078 |
| Ppu21I | YACGTR | 1 cut(s) | 1306 |
| PpuMI | RGGWCCY | 1 cut(s) | 1529 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1887 |
| Psp124BI | GAGCTC | 1 cut(s) | 1410 |
| Psp1406I | AACGTT | 1 cut(s) | 2070 |
| Psp5II | RGGWCCY | 1 cut(s) | 1529 |
| Psp6I | CCWGG | 1 cut(s) | 1348 |
| PspEI | GGTNACC | 1 cut(s) | 398 |
| PspFI | CCCAGC | 2 cut(s) | 994, 1546 |
| PspGI | CCWGG | 1 cut(s) | 1348 |
| PspN4I | GGNNCC | 5 cut(s) | 556, 1091, 1575, 1890, 2139 |
| PspPI | GGNCC | 7 cut(s) | 473, 992, 1057, 1062, 1529, 1889, 2188 |
| PspPPI | RGGWCCY | 1 cut(s) | 1529 |
| PstI | CTGCAG | 1 cut(s) | 1864 |
| PstNI | CAGNNNCTG | 4 cut(s) | 446, 1043, 1117, 2299 |
| PsuI | RGATCY | 2 cut(s) | 1765, 1864 |
| PsyI | GACNNNGTC | 2 cut(s) | 2042, 2087 |
| RsaI | GTAC | 6 cut(s) | 63, 206, 563, 1231, 1422, 1928 |
| RsaNI | GTAC | 6 cut(s) | 62, 205, 562, 1230, 1421, 1927 |
| RseI | CAYNNNNRTG | 4 cut(s) | 431, 852, 1037, 2126 |
| Rsr2I | CGGWCCG | 1 cut(s) | 2188 |
| RsrII | CGGWCCG | 1 cut(s) | 2188 |
| SacI | GAGCTC | 1 cut(s) | 1410 |
| SalI | GTCGAC | 1 cut(s) | 2081 |
| SaqAI | TTAA | 8 cut(s) | 456, 600, 1013, 1212, 1557, 1695, 2034, 2157 |
| Sau96I | GGNCC | 7 cut(s) | 473, 992, 1057, 1062, 1529, 1889, 2188 |
| ScaI | AGTACT | 1 cut(s) | 563 |
| SchI | GAGTC | 5 cut(s) | 260, 452, 705, 1928, 2078 |
| ScrFI | CCNGG | 4 cut(s) | 1350, 1578, 1869, 1924 |
| SduI | GDGCHC | 3 cut(s) | 751, 1410, 1576 |
| SfaNI | GCATC | 3 cut(s) | 175, 969, 1714 |
| SfcI | CTRYAG | 2 cut(s) | 1749, 1860 |
| SinI | GGWCC | 6 cut(s) | 473, 1057, 1062, 1529, 1889, 2188 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 431, 852, 1037, 2126 |
| SmlI | CTYRAG | 1 cut(s) | 727 |
| SmoI | CTYRAG | 1 cut(s) | 727 |
| SpeI | ACTAGT | 1 cut(s) | 2149 |
| Sse9I | AATT | 6 cut(s) | 7, 518, 532, 1615, 1711, 2005 |
| SspMI | CTAG | 6 cut(s) | 69, 633, 791, 1443, 1473, 2150 |
| SstI | GAGCTC | 1 cut(s) | 1410 |
| StyD4I | CCNGG | 4 cut(s) | 1348, 1576, 1867, 1922 |
| StyI | CCWWGG | 1 cut(s) | 1025 |
| TaiI | ACGT | 2 cut(s) | 1308, 2073 |
| TaqI | TCGA | 6 cut(s) | 331, 1289, 1538, 1655, 2082, 2106 |
| TasI | AATT | 6 cut(s) | 7, 518, 532, 1615, 1711, 2005 |
| TatI | WGTACW | 3 cut(s) | 204, 561, 1420 |
| TauI | GCSGC | 3 cut(s) | 1001, 2113, 2116 |
| Tru1I | TTAA | 8 cut(s) | 456, 600, 1013, 1212, 1557, 1695, 2034, 2157 |
| Tru9I | TTAA | 8 cut(s) | 456, 600, 1013, 1212, 1557, 1695, 2034, 2157 |
| TscAI | CASTG | 5 cut(s) | 804, 1024, 1044, 1122, 2224 |
| TseFI | GTSAC | 7 cut(s) | 162, 252, 398, 1744, 2044, 2062, 2266 |
| TseI | GCWGC | 9 cut(s) | 112, 236, 1043, 1295, 1313, 1595, 1661, 1724, 1859 |
| Tsp45I | GTSAC | 7 cut(s) | 162, 252, 398, 1744, 2044, 2062, 2266 |
| TspDTI | ATGAA | 9 cut(s) | 77, 90, 207, 281, 330, 511, 672, 798, 852 |
| TspGWI | ACGGA | 3 cut(s) | 1809, 1981, 2180 |
| TspRI | CASTG | 5 cut(s) | 804, 1024, 1044, 1122, 2224 |
| Tth111I | GACNNNGTC | 2 cut(s) | 2042, 2087 |
| VpaK11BI | GGWCC | 6 cut(s) | 473, 1057, 1062, 1529, 1889, 2188 |
| XapI | RAATTY | 1 cut(s) | 518 |
| XbaI | TCTAGA | 1 cut(s) | 1472 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 410 |
| XmiI | GTMKAC | 3 cut(s) | 1121, 2082, 2091 |
| XmnI | GAANNNNTTC | 1 cut(s) | 288 |
| XspI | CTAG | 6 cut(s) | 69, 633, 791, 1443, 1473, 2150 |
| ZrmI | AGTACT | 1 cut(s) | 563 |
| Zsp2I | ATGCAT | 1 cut(s) | 2262 |