subtilisin-like protease
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 1427 |
| AccI | GTMKAC | 1 cut(s) | 228 |
| AccII | CGCG | 1 cut(s) | 89 |
| AccIII | TCCGGA | 1 cut(s) | 1180 |
| AciI | CCGC | 5 cut(s) | 87, 89, 819, 1335, 1554 |
| AclWI | GGATC | 4 cut(s) | 1058, 1086, 1819, 2117 |
| AcoI | YGGCCR | 4 cut(s) | 84, 461, 786, 972 |
| AcsI | RAATTY | 5 cut(s) | 542, 650, 2053, 2208, 2286 |
| AcuI | CTGAAG | 1 cut(s) | 1296 |
| AfaI | GTAC | 3 cut(s) | 27, 1093, 2240 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 517, 1187, 1370, 1962, 2132 |
| AflIII | ACRYGT | 1 cut(s) | 1985 |
| AgsI | TTSAA | 8 cut(s) | 481, 595, 631, 1005, 1199, 1409, 1480, 2173 |
| AhlI | ACTAGT | 1 cut(s) | 1756 |
| AjnI | CCWGG | 4 cut(s) | 63, 974, 1125, 1591 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1248, 1280 |
| Alw21I | GWGCWC | 2 cut(s) | 586, 1863 |
| Alw26I | GTCTC | 4 cut(s) | 1693, 1737, 1993, 2085 |
| Alw44I | GTGCAC | 1 cut(s) | 1859 |
| AlwI | GGATC | 4 cut(s) | 1058, 1086, 1819, 2117 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 836, 1319 |
| Ama87I | CYCGRG | 3 cut(s) | 1539, 1660, 1882 |
| Aor13HI | TCCGGA | 1 cut(s) | 1180 |
| ApaLI | GTGCAC | 1 cut(s) | 1859 |
| ApoI | RAATTY | 5 cut(s) | 542, 650, 2053, 2208, 2286 |
| AspA2I | CCTAGG | 1 cut(s) | 493 |
| AspLEI | GCGC | 1 cut(s) | 1591 |
| AspS9I | GGNCC | 5 cut(s) | 497, 739, 1013, 1544, 1781 |
| AsuC2I | CCSGG | 2 cut(s) | 1883, 1884 |
| AsuHPI | GGTGA | 3 cut(s) | 381, 434, 548 |
| AsuNHI | GCTAGC | 1 cut(s) | 695 |
| AvaI | CYCGRG | 3 cut(s) | 1539, 1660, 1882 |
| AvaII | GGWCC | 3 cut(s) | 1013, 1544, 1781 |
| AvrII | CCTAGG | 1 cut(s) | 493 |
| BaeGI | GKGCMC | 1 cut(s) | 1863 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 1233, 1266, 2127, 2160 |
| BalI | TGGCCA | 2 cut(s) | 463, 974 |
| BanI | GGYRCC | 1 cut(s) | 1427 |
| BanII | GRGCYC | 1 cut(s) | 772 |
| Bbv12I | GWGCWC | 2 cut(s) | 586, 1863 |
| BccI | CCATC | 9 cut(s) | 217, 419, 514, 593, 866, 935, 1364, 1666, 1792 |
| BceAI | ACGGC | 3 cut(s) | 90, 610, 773 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 335, 369 |
| BciT130I | CCWGG | 4 cut(s) | 65, 976, 1127, 1593 |
| BciVI | GTATCC | 2 cut(s) | 1389, 1640 |
| BclI | TGATCA | 2 cut(s) | 156, 2332 |
| BcnI | CCSGG | 2 cut(s) | 1883, 1884 |
| BcoDI | GTCTC | 4 cut(s) | 1693, 1737, 1993, 2085 |
| BcuI | ACTAGT | 1 cut(s) | 1756 |
| BfaI | CTAG | 3 cut(s) | 494, 696, 1757 |
| BfmI | CTRYAG | 2 cut(s) | 235, 1209 |
| BfuI | GTATCC | 2 cut(s) | 1389, 1640 |
| BlnI | CCTAGG | 1 cut(s) | 493 |
| BmcAI | AGTACT | 1 cut(s) | 27 |
| Bme1390I | CCNGG | 6 cut(s) | 65, 976, 1127, 1593, 1883, 1884 |
| Bme18I | GGWCC | 3 cut(s) | 1013, 1544, 1781 |
| BmeT110I | CYCGRG | 3 cut(s) | 1539, 1660, 1882 |
| BmgT120I | GGNCC | 5 cut(s) | 497, 739, 1013, 1544, 1781 |
| BmiI | GGNNCC | 5 cut(s) | 769, 958, 1014, 1429, 1545 |
| BmrFI | CCNGG | 6 cut(s) | 65, 976, 1127, 1593, 1883, 1884 |
| BmrI | ACTGGG | 1 cut(s) | 402 |
| BmsI | GCATC | 3 cut(s) | 658, 1630, 1729 |
| BmtI | GCTAGC | 1 cut(s) | 699 |
| BmuI | ACTGGG | 1 cut(s) | 402 |
| BpmI | CTGGAG | 2 cut(s) | 1209, 1719 |
| Bpu10I | CCTNAGC | 1 cut(s) | 829 |
| BpuEI | CTTGAG | 3 cut(s) | 264, 1172, 1556 |
| BpuMI | CCSGG | 2 cut(s) | 1883, 1884 |
| Bsa29I | ATCGAT | 2 cut(s) | 74, 1670 |
| BsaBI | GATNNNNATC | 1 cut(s) | 168 |
| BsaI | GGTCTC | 3 cut(s) | 1737, 1993, 2085 |
| BsaWI | WCCGGW | 1 cut(s) | 1180 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 867, 897, 1765, 1795 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 517, 1187, 1370, 1962, 2132 |
| Bse118I | RCCGGY | 1 cut(s) | 707 |
| Bse1I | ACTGG | 5 cut(s) | 408, 500, 518, 958, 1733 |
| Bse3DI | GCAATG | 3 cut(s) | 554, 559, 945 |
| Bse8I | GATNNNNATC | 1 cut(s) | 168 |
| BseAI | TCCGGA | 1 cut(s) | 1180 |
| BseBI | CCWGG | 4 cut(s) | 65, 976, 1127, 1593 |
| BseCI | ATCGAT | 2 cut(s) | 74, 1670 |
| BseGI | GGATG | 5 cut(s) | 506, 931, 937, 1645, 1720 |
| BseJI | GATNNNNATC | 1 cut(s) | 168 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 517, 1187, 1370, 1962, 2132 |
| BseMI | GCAATG | 3 cut(s) | 554, 559, 945 |
| BseMII | CTCAG | 6 cut(s) | 398, 603, 843, 1297, 1704, 2062 |
| BseNI | ACTGG | 5 cut(s) | 408, 500, 518, 958, 1733 |
| BseRI | GAGGAG | 4 cut(s) | 372, 1671, 1697, 1806 |
| BseSI | GKGCMC | 1 cut(s) | 1863 |
| BsgI | GTGCAG | 3 cut(s) | 315, 1017, 1926 |
| Bsh1236I | CGCG | 1 cut(s) | 89 |
| BshNI | GGYRCC | 1 cut(s) | 1427 |
| BshVI | ATCGAT | 2 cut(s) | 74, 1670 |
| BsiHKAI | GWGCWC | 2 cut(s) | 586, 1863 |
| BsiHKCI | CYCGRG | 3 cut(s) | 1539, 1660, 1882 |
| BsiSI | CCGG | 3 cut(s) | 708, 1181, 1883 |
| BslFI | GGGAC | 5 cut(s) | 643, 766, 1026, 1557, 1974 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 517, 1187, 1370, 1962, 2132 |
| BsmAI | GTCTC | 4 cut(s) | 1693, 1737, 1993, 2085 |
| BsmFI | GGGAC | 5 cut(s) | 643, 766, 1026, 1557, 1974 |
| BsmI | GAATGC | 1 cut(s) | 1873 |
| Bso31I | GGTCTC | 3 cut(s) | 1737, 1993, 2085 |
| BsoBI | CYCGRG | 3 cut(s) | 1539, 1660, 1882 |
| Bsp1286I | GDGCHC | 3 cut(s) | 586, 772, 1863 |
| Bsp13I | TCCGGA | 1 cut(s) | 1180 |
| Bsp143I | GATC | 8 cut(s) | 156, 1050, 1078, 1451, 1748, 1811, 2122, 2332 |
| Bsp19I | CCATGG | 2 cut(s) | 1245, 1935 |
| BspACI | CCGC | 5 cut(s) | 87, 89, 819, 1335, 1554 |
| BspCNI | CTCAG | 6 cut(s) | 397, 604, 842, 1296, 1703, 2061 |
| BspDI | ATCGAT | 2 cut(s) | 74, 1670 |
| BspEI | TCCGGA | 1 cut(s) | 1180 |
| BspFNI | CGCG | 1 cut(s) | 89 |
| BspHI | TCATGA | 1 cut(s) | 172 |
| BspLI | GGNNCC | 5 cut(s) | 769, 958, 1014, 1429, 1545 |
| BspOI | GCTAGC | 1 cut(s) | 699 |
| BspPI | GGATC | 4 cut(s) | 1058, 1086, 1819, 2117 |
| BspT107I | GGYRCC | 1 cut(s) | 1427 |
| BspTNI | GGTCTC | 3 cut(s) | 1737, 1993, 2085 |
| BsrDI | GCAATG | 3 cut(s) | 554, 559, 945 |
| BsrFI | RCCGGY | 1 cut(s) | 707 |
| BsrI | ACTGG | 5 cut(s) | 408, 500, 518, 958, 1733 |
| BssAI | RCCGGY | 1 cut(s) | 707 |
| BssMI | GATC | 8 cut(s) | 156, 1050, 1078, 1451, 1748, 1811, 2122, 2332 |
| BssNAI | GTATAC | 1 cut(s) | 229 |
| BssT1I | CCWWGG | 5 cut(s) | 493, 742, 1046, 1245, 1935 |
| Bst1107I | GTATAC | 1 cut(s) | 229 |
| Bst2UI | CCWGG | 4 cut(s) | 65, 976, 1127, 1593 |
| BstC8I | GCNNGC | 6 cut(s) | 183, 697, 1069, 1333, 1505, 2277 |
| BstDEI | CTNAG | 7 cut(s) | 93, 384, 612, 829, 1283, 1690, 2048 |
| BstDSI | CCRYGG | 6 cut(s) | 87, 1074, 1245, 1518, 1935, 2178 |
| BstF5I | GGATG | 5 cut(s) | 506, 931, 937, 1645, 1720 |
| BstFNI | CGCG | 1 cut(s) | 89 |
| BstHHI | GCGC | 1 cut(s) | 1591 |
| BstKTI | GATC | 8 cut(s) | 159, 1053, 1081, 1454, 1751, 1814, 2125, 2335 |
| BstMAI | GTCTC | 4 cut(s) | 1693, 1737, 1993, 2085 |
| BstMBI | GATC | 8 cut(s) | 156, 1050, 1078, 1451, 1748, 1811, 2122, 2332 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 701, 776, 785, 825, 1316, 1341, 1523, 1562, 1649 |
| BstNI | CCWGG | 4 cut(s) | 65, 976, 1127, 1593 |
| BstNSI | RCATGY | 3 cut(s) | 1921, 1989, 2279 |
| BstSCI | CCNGG | 6 cut(s) | 63, 974, 1125, 1591, 1881, 1882 |
| BstSFI | CTRYAG | 2 cut(s) | 235, 1209 |
| BstSLI | GKGCMC | 1 cut(s) | 1863 |
| BstUI | CGCG | 1 cut(s) | 89 |
| BstX2I | RGATCY | 1 cut(s) | 1811 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 513, 2164 |
| BstYI | RGATCY | 1 cut(s) | 1811 |
| BstZ17I | GTATAC | 1 cut(s) | 229 |
| Bsu15I | ATCGAT | 2 cut(s) | 74, 1670 |
| BsuI | GTATCC | 2 cut(s) | 1389, 1640 |
| BsuTUI | ATCGAT | 2 cut(s) | 74, 1670 |
| BtgI | CCRYGG | 6 cut(s) | 87, 1074, 1245, 1518, 1935, 2178 |
| BtsCI | GGATG | 5 cut(s) | 506, 931, 937, 1645, 1720 |
| BtsI | GCAGTG | 3 cut(s) | 690, 699, 1715 |
| BtsIMutI | CAGTG | 8 cut(s) | 87, 511, 690, 699, 1365, 1715, 2145, 2335 |
| Cac8I | GCNNGC | 6 cut(s) | 183, 697, 1069, 1333, 1505, 2277 |
| CaiI | CAGNNNCTG | 2 cut(s) | 836, 1319 |
| CciI | TCATGA | 1 cut(s) | 172 |
| CfoI | GCGC | 1 cut(s) | 1591 |
| Cfr10I | RCCGGY | 1 cut(s) | 707 |
| Cfr13I | GGNCC | 5 cut(s) | 497, 739, 1013, 1544, 1781 |
| Cfr42I | CCGCGG | 1 cut(s) | 90 |
| Cfr9I | CCCGGG | 1 cut(s) | 1882 |
| ClaI | ATCGAT | 2 cut(s) | 74, 1670 |
| Csp6I | GTAC | 3 cut(s) | 26, 1092, 2239 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 175, 210 |
| CviQI | GTAC | 3 cut(s) | 26, 1092, 2239 |
| DdeI | CTNAG | 7 cut(s) | 93, 384, 612, 829, 1283, 1690, 2048 |
| DpnI | GATC | 8 cut(s) | 158, 1052, 1080, 1453, 1750, 1813, 2124, 2334 |
| DpnII | GATC | 8 cut(s) | 156, 1050, 1078, 1451, 1748, 1811, 2122, 2332 |
| EaeI | YGGCCR | 4 cut(s) | 84, 461, 786, 972 |
| Eco130I | CCWWGG | 5 cut(s) | 493, 742, 1046, 1245, 1935 |
| Eco147I | AGGCCT | 1 cut(s) | 492 |
| Eco24I | GRGCYC | 1 cut(s) | 772 |
| Eco31I | GGTCTC | 3 cut(s) | 1737, 1993, 2085 |
| Eco32I | GATATC | 1 cut(s) | 1463 |
| Eco47I | GGWCC | 3 cut(s) | 1013, 1544, 1781 |
| Eco57I | CTGAAG | 1 cut(s) | 1296 |
| Eco88I | CYCGRG | 3 cut(s) | 1539, 1660, 1882 |
| EcoO109I | RGGNCCY | 2 cut(s) | 739, 1013 |
| EcoRI | GAATTC | 2 cut(s) | 650, 2286 |
| EcoRII | CCWGG | 4 cut(s) | 63, 974, 1125, 1591 |
| EcoRV | GATATC | 1 cut(s) | 1463 |
| EcoT14I | CCWWGG | 5 cut(s) | 493, 742, 1046, 1245, 1935 |
| EcoT22I | ATGCAT | 1 cut(s) | 1923 |
| EcoT38I | GRGCYC | 1 cut(s) | 772 |
| ErhI | CCWWGG | 5 cut(s) | 493, 742, 1046, 1245, 1935 |
| FaqI | GGGAC | 5 cut(s) | 643, 766, 1026, 1557, 1974 |
| FbaI | TGATCA | 2 cut(s) | 156, 2332 |
| FblI | GTMKAC | 1 cut(s) | 228 |
| FokI | GGATG | 5 cut(s) | 493, 938, 944, 1652, 1707 |
| FriOI | GRGCYC | 1 cut(s) | 772 |
| FspBI | CTAG | 3 cut(s) | 494, 696, 1757 |
| GlaI | GCGC | 1 cut(s) | 1590 |
| GsuI | CTGGAG | 2 cut(s) | 1209, 1719 |
| HapII | CCGG | 3 cut(s) | 708, 1181, 1883 |
| HhaI | GCGC | 1 cut(s) | 1591 |
| Hin6I | GCGC | 1 cut(s) | 1589 |
| HinP1I | GCGC | 1 cut(s) | 1589 |
| HincII | GTYRAC | 2 cut(s) | 1414, 1827 |
| HindII | GTYRAC | 2 cut(s) | 1414, 1827 |
| HindIII | AAGCTT | 3 cut(s) | 266, 849, 1168 |
| HinfI | GANTC | 8 cut(s) | 164, 318, 467, 530, 1184, 1223, 1360, 1628 |
| HpaII | CCGG | 3 cut(s) | 708, 1181, 1883 |
| HphI | GGTGA | 3 cut(s) | 381, 434, 548 |
| Hpy166II | GTNNAC | 9 cut(s) | 112, 229, 793, 1143, 1414, 1600, 1793, 1827, 1861 |
| Hpy188III | TCNNGA | 9 cut(s) | 173, 252, 831, 895, 1181, 1188, 1254, 1662, 2200 |
| Hpy8I | GTNNAC | 9 cut(s) | 112, 229, 793, 1143, 1414, 1600, 1793, 1827, 1861 |
| HpyAV | CCTTC | 5 cut(s) | 482, 511, 1026, 2013, 2037 |
| HpyCH4IV | ACGT | 2 cut(s) | 586, 937 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 701, 776, 785, 825, 1316, 1341, 1523, 1562, 1649 |
| HpyF3I | CTNAG | 7 cut(s) | 93, 384, 612, 829, 1283, 1690, 2048 |
| HpySE526I | ACGT | 2 cut(s) | 586, 937 |
| HspAI | GCGC | 1 cut(s) | 1589 |
| Kpn2I | TCCGGA | 1 cut(s) | 1180 |
| Ksp22I | TGATCA | 2 cut(s) | 156, 2332 |
| KspI | CCGCGG | 1 cut(s) | 90 |
| Kzo9I | GATC | 8 cut(s) | 156, 1050, 1078, 1451, 1748, 1811, 2122, 2332 |
| LmnI | GCTCC | 4 cut(s) | 581, 767, 833, 962 |
| LweI | GCATC | 3 cut(s) | 658, 1630, 1729 |
| MaeI | CTAG | 3 cut(s) | 494, 696, 1757 |
| MaeII | ACGT | 2 cut(s) | 586, 937 |
| MaeIII | GTNAC | 7 cut(s) | 387, 718, 731, 888, 1300, 1759, 2077 |
| MalI | GATC | 8 cut(s) | 158, 1052, 1080, 1453, 1750, 1813, 2124, 2334 |
| MboI | GATC | 8 cut(s) | 156, 1050, 1078, 1451, 1748, 1811, 2122, 2332 |
| MboII | GAAGA | 2 cut(s) | 395, 497 |
| MfeI | CAATTG | 2 cut(s) | 1494, 2118 |
| MflI | RGATCY | 1 cut(s) | 1811 |
| MhlI | GDGCHC | 3 cut(s) | 586, 772, 1863 |
| MlsI | TGGCCA | 2 cut(s) | 463, 974 |
| MluNI | TGGCCA | 2 cut(s) | 463, 974 |
| MlyI | GAGTC | 3 cut(s) | 327, 476, 1193 |
| MmeI | TCCRAC | 1 cut(s) | 175 |
| Mox20I | TGGCCA | 2 cut(s) | 463, 974 |
| Mph1103I | ATGCAT | 1 cut(s) | 1923 |
| MroI | TCCGGA | 1 cut(s) | 1180 |
| MscI | TGGCCA | 2 cut(s) | 463, 974 |
| MseI | TTAA | 4 cut(s) | 546, 1236, 2019, 2205 |
| MslI | CAYNNNNRTG | 2 cut(s) | 678, 1866 |
| Msp20I | TGGCCA | 2 cut(s) | 463, 974 |
| MspA1I | CMGCKG | 2 cut(s) | 89, 1316 |
| MspI | CCGG | 3 cut(s) | 708, 1181, 1883 |
| MspR9I | CCNGG | 6 cut(s) | 65, 976, 1127, 1593, 1883, 1884 |
| MunI | CAATTG | 2 cut(s) | 1494, 2118 |
| Mva1269I | GAATGC | 1 cut(s) | 1873 |
| MvaI | CCWGG | 4 cut(s) | 65, 976, 1127, 1593 |
| MvnI | CGCG | 1 cut(s) | 89 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 701, 776, 785, 825, 1316, 1341, 1523, 1562, 1649 |
| NciI | CCSGG | 2 cut(s) | 1883, 1884 |
| NcoI | CCATGG | 2 cut(s) | 1245, 1935 |
| NdeII | GATC | 8 cut(s) | 156, 1050, 1078, 1451, 1748, 1811, 2122, 2332 |
| NheI | GCTAGC | 1 cut(s) | 695 |
| NlaIV | GGNNCC | 5 cut(s) | 769, 958, 1014, 1429, 1545 |
| NmeAIII | GCCGAG | 1 cut(s) | 1831 |
| NmuCI | GTSAC | 4 cut(s) | 387, 888, 1759, 2077 |
| NsiI | ATGCAT | 1 cut(s) | 1923 |
| NspI | RCATGY | 3 cut(s) | 1921, 1989, 2279 |
| PaeI | GCATGC | 1 cut(s) | 2279 |
| PagI | TCATGA | 1 cut(s) | 172 |
| PceI | AGGCCT | 1 cut(s) | 492 |
| PciI | ACATGT | 1 cut(s) | 1985 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 1087, 1667 |
| PctI | GAATGC | 1 cut(s) | 1873 |
| PfeI | GAWTC | 5 cut(s) | 164, 530, 1223, 1360, 1628 |
| PleI | GAGTC | 3 cut(s) | 326, 475, 1192 |
| PpsI | GAGTC | 3 cut(s) | 326, 475, 1192 |
| PpuMI | RGGWCCY | 1 cut(s) | 1013 |
| PscI | ACATGT | 1 cut(s) | 1985 |
| Psp5II | RGGWCCY | 1 cut(s) | 1013 |
| Psp6I | CCWGG | 4 cut(s) | 63, 974, 1125, 1591 |
| PspGI | CCWGG | 4 cut(s) | 63, 974, 1125, 1591 |
| PspN4I | GGNNCC | 5 cut(s) | 769, 958, 1014, 1429, 1545 |
| PspPI | GGNCC | 5 cut(s) | 497, 739, 1013, 1544, 1781 |
| PspPPI | RGGWCCY | 1 cut(s) | 1013 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 2222, 2254 |
| PstNI | CAGNNNCTG | 2 cut(s) | 836, 1319 |
| PsuI | RGATCY | 1 cut(s) | 1811 |
| PvuII | CAGCTG | 1 cut(s) | 1316 |
| RsaI | GTAC | 3 cut(s) | 27, 1093, 2240 |
| RsaNI | GTAC | 3 cut(s) | 26, 1092, 2239 |
| RseI | CAYNNNNRTG | 2 cut(s) | 678, 1866 |
| SacII | CCGCGG | 1 cut(s) | 90 |
| SaqAI | TTAA | 4 cut(s) | 546, 1236, 2019, 2205 |
| Sau3AI | GATC | 8 cut(s) | 156, 1050, 1078, 1451, 1748, 1811, 2122, 2332 |
| Sau96I | GGNCC | 5 cut(s) | 497, 739, 1013, 1544, 1781 |
| ScaI | AGTACT | 1 cut(s) | 27 |
| SchI | GAGTC | 3 cut(s) | 327, 476, 1193 |
| ScrFI | CCNGG | 6 cut(s) | 65, 976, 1127, 1593, 1883, 1884 |
| SduI | GDGCHC | 3 cut(s) | 586, 772, 1863 |
| SfaNI | GCATC | 3 cut(s) | 658, 1630, 1729 |
| SfcI | CTRYAG | 2 cut(s) | 235, 1209 |
| Sfr303I | CCGCGG | 1 cut(s) | 90 |
| SgrBI | CCGCGG | 1 cut(s) | 90 |
| SinI | GGWCC | 3 cut(s) | 1013, 1544, 1781 |
| SmaI | CCCGGG | 1 cut(s) | 1884 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 678, 1866 |
| SmlI | CTYRAG | 3 cut(s) | 279, 1151, 1571 |
| SmoI | CTYRAG | 3 cut(s) | 279, 1151, 1571 |
| SpeI | ACTAGT | 1 cut(s) | 1756 |
| SphI | GCATGC | 1 cut(s) | 2279 |
| SseBI | AGGCCT | 1 cut(s) | 492 |
| SsiI | CCGC | 5 cut(s) | 87, 89, 819, 1335, 1554 |
| SspI | AATATT | 2 cut(s) | 400, 1512 |
| SspMI | CTAG | 3 cut(s) | 494, 696, 1757 |
| StuI | AGGCCT | 1 cut(s) | 492 |
| StyD4I | CCNGG | 6 cut(s) | 63, 974, 1125, 1591, 1881, 1882 |
| StyI | CCWWGG | 5 cut(s) | 493, 742, 1046, 1245, 1935 |
| TaiI | ACGT | 2 cut(s) | 589, 940 |
| TaqI | TCGA | 9 cut(s) | 74, 316, 781, 896, 1081, 1670, 1747, 1839, 2199 |
| TatI | WGTACW | 1 cut(s) | 25 |
| TauI | GCSGC | 2 cut(s) | 89, 92 |
| TfiI | GAWTC | 5 cut(s) | 164, 530, 1223, 1360, 1628 |
| Tru1I | TTAA | 4 cut(s) | 546, 1236, 2019, 2205 |
| Tru9I | TTAA | 4 cut(s) | 546, 1236, 2019, 2205 |
| TscAI | CASTG | 7 cut(s) | 87, 518, 697, 706, 1372, 1722, 2145 |
| TseFI | GTSAC | 4 cut(s) | 387, 888, 1759, 2077 |
| Tsp45I | GTSAC | 4 cut(s) | 387, 888, 1759, 2077 |
| TspDTI | ATGAA | 9 cut(s) | 498, 643, 873, 948, 953, 1524, 1620, 2124, 2243 |
| TspGWI | ACGGA | 4 cut(s) | 176, 287, 1091, 2092 |
| TspMI | CCCGGG | 1 cut(s) | 1882 |
| TspRI | CASTG | 7 cut(s) | 87, 518, 697, 706, 1372, 1722, 2145 |
| VneI | GTGCAC | 1 cut(s) | 1859 |
| VpaK11BI | GGWCC | 3 cut(s) | 1013, 1544, 1781 |
| XapI | RAATTY | 5 cut(s) | 542, 650, 2053, 2208, 2286 |
| XceI | RCATGY | 3 cut(s) | 1921, 1989, 2279 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 415 |
| XmaI | CCCGGG | 1 cut(s) | 1882 |
| XmaJI | CCTAGG | 1 cut(s) | 493 |
| XmiI | GTMKAC | 1 cut(s) | 228 |
| XspI | CTAG | 3 cut(s) | 494, 696, 1757 |
| ZrmI | AGTACT | 1 cut(s) | 27 |
| Zsp2I | ATGCAT | 1 cut(s) | 1923 |