FvH4_5g10502

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001127
Physical Location & Seq
Reverse (-)
5972525 .. 5976224
3700 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g10502.t1

Sequence Viewer

Length: 2343 bp
ATGAGCATCCCAATGAGTACAGTAGTACTTGGTTTGTTATGCCTTCTTTGTGTTCTTAATGAGCAGGGAATATCGATAACAGTGGTGGCAGCTAAGACTAATAGCCATGTTCACATTGTTTATCTGGGAGAGAGGCAACATGACAATCCCAAGTTGATCACTGATTCTCATCATGATTTGCTTGCCACCATAGTTGGAAGTAAAAGTTTGGCCTCAAAATTGATGGTATACAGTTACAGACATGGTTTCTCTGGATTTGCAGCCAAGCTTACAGCGGCTCAAGCCCAAAAATTTGCAGAGTTGCCTGATGTTGTTCGAGTCATACCAAATACTCTACACAAGCTGCAAACTAGTAGGAGTTGGGATTTTCTTGGCCTCTCTCCTCTTTCACCTTCAAGCAATATTCTTCATAGCAGCAACATGGGTGATGGTGTTATCATAGGTGTCCTTGACACAGGTATATGGCCAGAGTCTAAATCTTTCAATGAAGAAGGCTTAGGGTCAGTTCCATCCCGCTGGAAGGGTGTTTGTAAATCTGGGGAAAAGTTTAATGCCACATTGCATTGCAATAGAAAAATTATCGGAGCACGTTGGTTCAACGATGGAATACTTGCTGAGTATGGAAAACCGTTGAACACATCGAAAAGAACTGAATTCATGTCCCCAAGAGATGCACATGGCCATGGCACTCACACTGCTAGCACTGCAGCTGGTTCTTTTGTGACTAATGTTAGTTACAAGGGCCTTGGTCATGGGACTATTAGAGGGGGTGCCCCAAATGCTAGATTGGCCATTTACAAGGTTTGCTGGAATGTGCTTGGAGGTCAATGCTCAGCAGCTGATATGCTCAAAGGTTTTGATGAAGCTATACATGATGGAGTTGATGTATTGTCACTCTCAATTGGGGGTTCAGTTCCACTCTTTTCTGATGTGGATGAGCGTGATGGAATTTCAACTGGCTCCTTTCATGCTGTAGCCAGGGGAATTACTGTTGTTTGTGCAGCTTCAAATGATGGACCTTCGGCGCAGACAGTTCAGAACATATCACCATGGATCATAACTGTTGCAGCAAGCACCATGGATCGAGCATTTCCTACTTCCATAACTTTAGGAAATAACAAAACTTTCCTGGGTCAAGCTATGTTTACAGGACTTGAGATTGGTTTTACAAGCTTGATATATCCGGAGTCTAAAGGGCTTTACCCTACAGCTACAGGTGTCTGTGATTCCCTCTCACTTAACAACACCATGGTATCTGGAATGGTGGTTCTATGCTTCACTTCACTCGGTCGTAGGATAGATGTAACAACAGCTTCAGATGCTGTGAAACAAGCTGGCGGTGTTGGCCTAATCATAGCAAAGAATCCAACTGACGGCTTGTATCCCTGCAGCGATGACTTTCCTTGCATTGAAGTTGACTATGAGATTGGCACCCGAATAGTGTTTTATATCCGATCTACCAGATATCCTCTTGTGAAGTTGACCCCTCCTACAACAATTGTGGGCAAGCCAATATCGGCCAAGGTTGCCTATTTTTCATCTCGCGGACCAAACTCTGCTGCACCAGCAATTCTCAAGCCGGATGTAACTGCACCTGGTGTGAACATATTAGCTGCAACTTCTCCACTTGATTCATTTGCGGATGCTGGATATGTTATGCACTCGGGAACATCGATGTCTACTCCTCATGTCGCAGGCATTGTGGCTCTCATCAAAGCAGTGCATCCTAACTGGTCTCCAGCAGCCATTCGATCAGCGCTAGTCACAACTGCATGGAGCAATGGTCCATCTCGTTTACCAATTTTTGCTGAGGGATCTCCTCAAAAGTTGGCTAATCCATTCGACTTTGGAGGTGGCATTGTGAACCCGAATGCAGCATCAAACCCGGGTCTTGTATATGACATGGGCGCAGCAGACTACATGCATTATCTATGTGCCATGGACTACAACAACTCTGACATTACAAGGCTCACAGGCCAAGCCACAACATGTCCCATGAAGAGACCTTCTCTTTTGGACATTAACCTACCTTCCATAACCATACCAAGCCTCGGAAATTCCATTACTGTAACAAGAACTGTGACAAATGTAGGAGCCCCTAAATCCGTTTATGAAGCCACAATTGATCCTCCATTGGGAACAGTGGTATCTGTAAAACCCAATCTTCTGGTATTCAACTCCACGGTAAAGAAACTCACTTTCGAGATTGTGATTTCCACAACCCACCAGATGAACACAGGGTACTATTTTGGAAGCCTTACTTGGACTGATAGAGTGCATGCTGTGAGAATTCCCTTGTCAGTGAGGACAAATTTTTTACAACATTTTGCTGATGATAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

781

Amino Acids

83.01

Weight (kDa)

8.29

Isoelectric Point (pI)

30.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 37 - 115 7.2e-16 Peptidase inhibitor I9
Peptidase_S8 PF00082 142 - 597 2.5e-46 Subtilase family
fn3_6 PF17766 673 - 768 5e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 770, 1430
AccI GTMKAC 2 cut(s) 228, 1679
AccII CGCG 1 cut(s) 1545
AccIII TCCGGA 1 cut(s) 1183
AciI CCGC 5 cut(s) 275, 514, 1338, 1545, 1640
AclWI GGATC 4 cut(s) 1061, 1089, 1822, 2120
AcoI YGGCCR 4 cut(s) 464, 679, 789, 1518
AcsI RAATTY 6 cut(s) 290, 653, 948, 2056, 2289, 2311
AcuI CTGAAG 1 cut(s) 1299
AdeI CACNNNGTG 1 cut(s) 1598
AfaI GTAC 3 cut(s) 19, 27, 2243
AfeI AGCGCT 1 cut(s) 1758
AfiI CCNNNNNNNGG 4 cut(s) 520, 1373, 2051, 2135
AflIII ACRYGT 1 cut(s) 1988
AgsI TTSAA 8 cut(s) 396, 484, 598, 634, 954, 1008, 1412, 2176
AhlI ACTAGT 1 cut(s) 350
AjnI CCWGG 3 cut(s) 977, 1128, 1594
AjuI GAANNNNNNNTTGG 2 cut(s) 2245, 2277
AloI GAACNNNNNNTCC 2 cut(s) 1251, 1283
Alw21I GWGCWC 1 cut(s) 589
Alw26I GTCTC 2 cut(s) 1740, 1996
AlwI GGATC 4 cut(s) 1061, 1089, 1822, 2120
AlwNI CAGNNNCTG 2 cut(s) 839, 1322
Ama87I CYCGRG 2 cut(s) 1663, 1885
Aor13HI TCCGGA 1 cut(s) 1183
Aor51HI AGCGCT 1 cut(s) 1758
AoxI GGCC 9 cut(s) 210, 373, 464, 679, 742, 789, 1345, 1518, 1975
ApoI RAATTY 6 cut(s) 290, 653, 948, 2056, 2289, 2311
AspLEI GCGC 3 cut(s) 1027, 1759, 1910
AspS9I GGNCC 4 cut(s) 742, 1016, 1547, 1784
AsuC2I CCSGG 2 cut(s) 1886, 1887
AsuHPI GGTGA 3 cut(s) 381, 437, 1038
AsuNHI GCTAGC 1 cut(s) 698
AvaI CYCGRG 2 cut(s) 1663, 1885
AvaII GGWCC 3 cut(s) 1016, 1547, 1784
BaeGI GKGCMC 1 cut(s) 775
BaeI ACNNNNGTAYC 4 cut(s) 1236, 1269, 2130, 2163
BalI TGGCCA 3 cut(s) 466, 681, 791
BanI GGYRCC 2 cut(s) 770, 1430
BanII GRGCYC 1 cut(s) 2098
Bbv12I GWGCWC 1 cut(s) 589
BbvCI CCTCAGC 1 cut(s) 1809
BccI CCATC 8 cut(s) 217, 422, 517, 596, 869, 938, 1007, 1795
BceAI ACGGC 1 cut(s) 1390
BciT130I CCWGG 3 cut(s) 979, 1130, 1596
BciVI GTATCC 1 cut(s) 1392
BclI TGATCA 1 cut(s) 156
BcnI CCSGG 2 cut(s) 1886, 1887
BcoDI GTCTC 2 cut(s) 1740, 1996
BcuI ACTAGT 1 cut(s) 350
BfaI CTAG 4 cut(s) 351, 699, 783, 1760
BfmI CTRYAG 5 cut(s) 705, 972, 1206, 1212, 1387
BfoI RGCGCY 1 cut(s) 1760
BfuI GTATCC 1 cut(s) 1392
BlpI GCTNAGC 1 cut(s) 832
BmcAI AGTACT 1 cut(s) 27
Bme1390I CCNGG 5 cut(s) 979, 1130, 1596, 1886, 1887
Bme18I GGWCC 3 cut(s) 1016, 1547, 1784
BmeT110I CYCGRG 2 cut(s) 1663, 1885
BmgT120I GGNCC 4 cut(s) 742, 1016, 1547, 1784
BmiI GGNNCC 4 cut(s) 772, 961, 1432, 2095
BmrFI CCNGG 5 cut(s) 979, 1130, 1596, 1886, 1887
BmsI GCATC 6 cut(s) 15, 661, 1309, 1633, 1732, 1886
BmtI GCTAGC 1 cut(s) 702
BplI GAGNNNNNCTC 2 cut(s) 1993, 2025
BpmI CTGGAG 1 cut(s) 1722
Bpu10I CCTNAGC 2 cut(s) 496, 1809
Bpu1102I GCTNAGC 1 cut(s) 832
BpuEI CTTGAG 3 cut(s) 264, 1175, 1559
BpuMI CCSGG 2 cut(s) 1886, 1887
Bsa29I ATCGAT 2 cut(s) 74, 1673
BsaBI GATNNNNATC 1 cut(s) 168
BsaI GGTCTC 2 cut(s) 1740, 1996
BsaWI WCCGGW 1 cut(s) 1183
BsaXI ACNNNNNCTCC 4 cut(s) 870, 900, 1768, 1798
Bsc4I CCNNNNNNNGG 4 cut(s) 520, 1373, 2051, 2135
Bse1I ACTGG 2 cut(s) 961, 1736
Bse3DI GCAATG 3 cut(s) 557, 562, 1786
Bse8I GATNNNNATC 1 cut(s) 168
BseAI TCCGGA 1 cut(s) 1183
BseBI CCWGG 3 cut(s) 979, 1130, 1596
BseCI ATCGAT 2 cut(s) 74, 1673
BseGI GGATG 6 cut(s) 6, 509, 940, 1588, 1648, 1723
BseJI GATNNNNATC 1 cut(s) 168
BseLI CCNNNNNNNGG 4 cut(s) 520, 1373, 2051, 2135
BseMI GCAATG 3 cut(s) 557, 562, 1786
BseMII CTCAG 3 cut(s) 606, 846, 1800
BseNI ACTGG 2 cut(s) 961, 1736
BseRI GAGGAG 3 cut(s) 372, 1674, 1809
BseSI GKGCMC 1 cut(s) 775
BsgI GTGCAG 3 cut(s) 1020, 1545, 1575
Bsh1236I CGCG 1 cut(s) 1545
Bsh1285I CGRYCG 1 cut(s) 1291
BshFI GGCC 9 cut(s) 212, 375, 466, 681, 744, 791, 1347, 1520, 1977
BshNI GGYRCC 2 cut(s) 770, 1430
BshVI ATCGAT 2 cut(s) 74, 1673
BsiEI CGRYCG 1 cut(s) 1291
BsiHKAI GWGCWC 1 cut(s) 589
BsiHKCI CYCGRG 2 cut(s) 1663, 1885
BsiSI CCGG 3 cut(s) 1184, 1580, 1886
BslFI GGGAC 3 cut(s) 646, 769, 1977
BslI CCNNNNNNNGG 4 cut(s) 520, 1373, 2051, 2135
BsmAI GTCTC 2 cut(s) 1740, 1996
BsmFI GGGAC 3 cut(s) 646, 769, 1977
BsmI GAATGC 1 cut(s) 1876
BsnI GGCC 9 cut(s) 212, 375, 466, 681, 744, 791, 1347, 1520, 1977
Bso31I GGTCTC 2 cut(s) 1740, 1996
BsoBI CYCGRG 2 cut(s) 1663, 1885
Bsp1286I GDGCHC 3 cut(s) 589, 775, 2098
Bsp13I TCCGGA 1 cut(s) 1183
Bsp143I GATC 7 cut(s) 156, 1053, 1081, 1454, 1751, 1814, 2125
Bsp1720I GCTNAGC 1 cut(s) 832
Bsp19I CCATGG 5 cut(s) 682, 1049, 1077, 1248, 1938
BspACI CCGC 5 cut(s) 275, 514, 1338, 1545, 1640
BspANI GGCC 9 cut(s) 212, 375, 466, 681, 744, 791, 1347, 1520, 1977
BspCNI CTCAG 3 cut(s) 607, 845, 1801
BspDI ATCGAT 2 cut(s) 74, 1673
BspEI TCCGGA 1 cut(s) 1183
BspFNI CGCG 1 cut(s) 1545
BspHI TCATGA 1 cut(s) 172
BspLI GGNNCC 4 cut(s) 772, 961, 1432, 2095
BspMAI CTGCAG 2 cut(s) 709, 1391
BspOI GCTAGC 1 cut(s) 702
BspPI GGATC 4 cut(s) 1061, 1089, 1822, 2120
BspT107I GGYRCC 2 cut(s) 770, 1430
BspTNI GGTCTC 2 cut(s) 1740, 1996
BsrDI GCAATG 3 cut(s) 557, 562, 1786
BsrI ACTGG 2 cut(s) 961, 1736
BssMI GATC 7 cut(s) 156, 1053, 1081, 1454, 1751, 1814, 2125
BssNAI GTATAC 1 cut(s) 229
BssT1I CCWWGG 7 cut(s) 682, 745, 1049, 1077, 1248, 1521, 1938
Bst1107I GTATAC 1 cut(s) 229
Bst2UI CCWGG 3 cut(s) 979, 1130, 1596
Bst6I CTCTTC 1 cut(s) 1994
BstC8I GCNNGC 7 cut(s) 183, 700, 1072, 1336, 1508, 1696, 2280
BstDEI CTNAG 5 cut(s) 93, 496, 615, 832, 1809
BstDSI CCRYGG 6 cut(s) 682, 1049, 1077, 1248, 1938, 2181
BstF5I GGATG 6 cut(s) 6, 509, 940, 1588, 1648, 1723
BstFNI CGCG 1 cut(s) 1545
BstH2I RGCGCY 1 cut(s) 1760
BstHHI GCGC 3 cut(s) 1027, 1759, 1910
BstKTI GATC 7 cut(s) 159, 1056, 1084, 1457, 1754, 1817, 2128
BstMAI GTCTC 2 cut(s) 1740, 1996
BstMBI GATC 7 cut(s) 156, 1053, 1081, 1454, 1751, 1814, 2125
BstMCI CGRYCG 1 cut(s) 1291
BstMWI GCNNNNNNNGC 8 cut(s) 281, 704, 779, 788, 1319, 1344, 1526, 1565
BstNI CCWGG 3 cut(s) 979, 1130, 1596
BstNSI RCATGY 3 cut(s) 1924, 1992, 2282
BstSCI CCNGG 5 cut(s) 977, 1128, 1594, 1884, 1885
BstSFI CTRYAG 5 cut(s) 705, 972, 1206, 1212, 1387
BstSLI GKGCMC 1 cut(s) 775
BstUI CGCG 1 cut(s) 1545
BstX2I RGATCY 1 cut(s) 1814
BstXI CCANNNNNNTGG 2 cut(s) 516, 2167
BstYI RGATCY 1 cut(s) 1814
BstZ17I GTATAC 1 cut(s) 229
Bsu15I ATCGAT 2 cut(s) 74, 1673
BsuI GTATCC 1 cut(s) 1392
BsuRI GGCC 9 cut(s) 212, 375, 466, 681, 744, 791, 1347, 1520, 1977
BsuTUI ATCGAT 2 cut(s) 74, 1673
BtgI CCRYGG 6 cut(s) 682, 1049, 1077, 1248, 1938, 2181
BtgZI GCGATG 1 cut(s) 1407
BtsCI GGATG 6 cut(s) 6, 509, 940, 1588, 1648, 1723
BtsI GCAGTG 3 cut(s) 693, 702, 1725
BtsIMutI CAGTG 7 cut(s) 87, 159, 693, 702, 1725, 2148, 2307
Cac8I GCNNGC 7 cut(s) 183, 700, 1072, 1336, 1508, 1696, 2280
CaiI CAGNNNCTG 2 cut(s) 839, 1322
CciI TCATGA 1 cut(s) 172
CfoI GCGC 3 cut(s) 1027, 1759, 1910
Cfr13I GGNCC 4 cut(s) 742, 1016, 1547, 1784
Cfr9I CCCGGG 1 cut(s) 1885
ClaI ATCGAT 2 cut(s) 74, 1673
CsiI ACCWGGT 1 cut(s) 1594
Csp6I GTAC 3 cut(s) 18, 26, 2242
CspCI CAANNNNNGTGG 4 cut(s) 175, 210, 1482, 1517
CviQI GTAC 3 cut(s) 18, 26, 2242
DdeI CTNAG 5 cut(s) 93, 496, 615, 832, 1809
DpnI GATC 7 cut(s) 158, 1055, 1083, 1456, 1753, 1816, 2127
DpnII GATC 7 cut(s) 156, 1053, 1081, 1454, 1751, 1814, 2125
DraIII CACNNNGTG 1 cut(s) 1598
EaeI YGGCCR 4 cut(s) 464, 679, 789, 1518
Eam1104I CTCTTC 1 cut(s) 1994
EarI CTCTTC 1 cut(s) 1994
Eco130I CCWWGG 7 cut(s) 682, 745, 1049, 1077, 1248, 1521, 1938
Eco24I GRGCYC 1 cut(s) 2098
Eco31I GGTCTC 2 cut(s) 1740, 1996
Eco32I GATATC 1 cut(s) 1466
Eco47I GGWCC 3 cut(s) 1016, 1547, 1784
Eco47III AGCGCT 1 cut(s) 1758
Eco57I CTGAAG 1 cut(s) 1299
Eco88I CYCGRG 2 cut(s) 1663, 1885
EcoO109I RGGNCCY 1 cut(s) 742
EcoRI GAATTC 2 cut(s) 653, 2289
EcoRII CCWGG 3 cut(s) 977, 1128, 1594
EcoRV GATATC 1 cut(s) 1466
EcoT14I CCWWGG 7 cut(s) 682, 745, 1049, 1077, 1248, 1521, 1938
EcoT22I ATGCAT 1 cut(s) 1926
EcoT38I GRGCYC 1 cut(s) 2098
ErhI CCWWGG 7 cut(s) 682, 745, 1049, 1077, 1248, 1521, 1938
FalI AAGNNNNNCTT 2 cut(s) 2245, 2277
FaqI GGGAC 3 cut(s) 646, 769, 1977
FauI CCCGC 1 cut(s) 521
FbaI TGATCA 1 cut(s) 156
FblI GTMKAC 2 cut(s) 228, 1679
FokI GGATG 5 cut(s) 496, 947, 1595, 1655, 1710
FriOI GRGCYC 1 cut(s) 2098
FspBI CTAG 4 cut(s) 351, 699, 783, 1760
GlaI GCGC 3 cut(s) 1026, 1758, 1909
GsuI CTGGAG 1 cut(s) 1722
HaeII RGCGCY 1 cut(s) 1760
HaeIII GGCC 9 cut(s) 212, 375, 466, 681, 744, 791, 1347, 1520, 1977
HapII CCGG 3 cut(s) 1184, 1580, 1886
HhaI GCGC 3 cut(s) 1027, 1759, 1910
Hin6I GCGC 3 cut(s) 1025, 1757, 1908
HinP1I GCGC 3 cut(s) 1025, 1757, 1908
HincII GTYRAC 2 cut(s) 1417, 1482
HindII GTYRAC 2 cut(s) 1417, 1482
HindIII AAGCTT 2 cut(s) 266, 1171
HinfI GANTC 7 cut(s) 164, 318, 470, 1187, 1226, 1363, 1631
HpaII CCGG 3 cut(s) 1184, 1580, 1886
HphI GGTGA 3 cut(s) 381, 437, 1038
Hpy166II GTNNAC 9 cut(s) 112, 229, 1146, 1417, 1482, 1603, 1680, 1796, 1864
Hpy188I TCNGA 7 cut(s) 584, 928, 1038, 1318, 1454, 1957, 2054
Hpy188III TCNNGA 6 cut(s) 173, 252, 1184, 1257, 1665, 2203
Hpy8I GTNNAC 9 cut(s) 112, 229, 1146, 1417, 1482, 1603, 1680, 1796, 1864
HpyAV CCTTC 7 cut(s) 53, 402, 485, 514, 1029, 2016, 2040
HpyCH4IV ACGT 1 cut(s) 589
HpyF10VI GCNNNNNNNGC 8 cut(s) 281, 704, 779, 788, 1319, 1344, 1526, 1565
HpyF3I CTNAG 5 cut(s) 93, 496, 615, 832, 1809
HpySE526I ACGT 1 cut(s) 589
HspAI GCGC 3 cut(s) 1025, 1757, 1908
Kpn2I TCCGGA 1 cut(s) 1183
Ksp22I TGATCA 1 cut(s) 156
Kzo9I GATC 7 cut(s) 156, 1053, 1081, 1454, 1751, 1814, 2125
LmnI GCTCC 4 cut(s) 584, 965, 1776, 2093
LweI GCATC 6 cut(s) 15, 661, 1309, 1633, 1732, 1886
MabI ACCWGGT 1 cut(s) 1594
MaeI CTAG 4 cut(s) 351, 699, 783, 1760
MaeII ACGT 1 cut(s) 589
MaeIII GTNAC 9 cut(s) 233, 721, 734, 891, 1303, 1585, 1762, 2068, 2080
MalI GATC 7 cut(s) 158, 1055, 1083, 1456, 1753, 1816, 2127
MboI GATC 7 cut(s) 156, 1053, 1081, 1454, 1751, 1814, 2125
MboII GAAGA 4 cut(s) 398, 500, 2011, 2156
MfeI CAATTG 3 cut(s) 900, 1497, 2121
MflI RGATCY 1 cut(s) 1814
MhlI GDGCHC 3 cut(s) 589, 775, 2098
MlsI TGGCCA 3 cut(s) 466, 681, 791
MluNI TGGCCA 3 cut(s) 466, 681, 791
MlyI GAGTC 3 cut(s) 327, 479, 1196
MmeI TCCRAC 2 cut(s) 175, 1391
Mox20I TGGCCA 3 cut(s) 466, 681, 791
Mph1103I ATGCAT 1 cut(s) 1926
MroI TCCGGA 1 cut(s) 1183
MscI TGGCCA 3 cut(s) 466, 681, 791
MseI TTAA 4 cut(s) 57, 549, 1239, 2022
MslI CAYNNNNRTG 2 cut(s) 11, 681
Msp20I TGGCCA 3 cut(s) 466, 681, 791
MspA1I CMGCKG 4 cut(s) 275, 516, 710, 839
MspI CCGG 3 cut(s) 1184, 1580, 1886
MspR9I CCNGG 5 cut(s) 979, 1130, 1596, 1886, 1887
MunI CAATTG 3 cut(s) 900, 1497, 2121
Mva1269I GAATGC 1 cut(s) 1876
MvaI CCWGG 3 cut(s) 979, 1130, 1596
MvnI CGCG 1 cut(s) 1545
MwoI GCNNNNNNNGC 8 cut(s) 281, 704, 779, 788, 1319, 1344, 1526, 1565
NciI CCSGG 2 cut(s) 1886, 1887
NcoI CCATGG 5 cut(s) 682, 1049, 1077, 1248, 1938
NdeII GATC 7 cut(s) 156, 1053, 1081, 1454, 1751, 1814, 2125
NheI GCTAGC 1 cut(s) 698
NlaIV GGNNCC 4 cut(s) 772, 961, 1432, 2095
NmuCI GTSAC 4 cut(s) 721, 891, 1762, 2080
NsiI ATGCAT 1 cut(s) 1926
NspI RCATGY 3 cut(s) 1924, 1992, 2282
PaeI GCATGC 1 cut(s) 2282
PagI TCATGA 1 cut(s) 172
PciI ACATGT 1 cut(s) 1988
PcsI WCGNNNNNNNCGW 1 cut(s) 1670
PctI GAATGC 1 cut(s) 1876
PfeI GAWTC 4 cut(s) 164, 1226, 1363, 1631
PleI GAGTC 3 cut(s) 326, 478, 1195
PpsI GAGTC 3 cut(s) 326, 478, 1195
PscI ACATGT 1 cut(s) 1988
Psp6I CCWGG 3 cut(s) 977, 1128, 1594
PspGI CCWGG 3 cut(s) 977, 1128, 1594
PspN4I GGNNCC 4 cut(s) 772, 961, 1432, 2095
PspPI GGNCC 4 cut(s) 742, 1016, 1547, 1784
PsrI GAACNNNNNNTAC 2 cut(s) 2225, 2257
PstI CTGCAG 2 cut(s) 709, 1391
PstNI CAGNNNCTG 2 cut(s) 839, 1322
PsuI RGATCY 1 cut(s) 1814
PvuII CAGCTG 2 cut(s) 710, 839
RsaI GTAC 3 cut(s) 19, 27, 2243
RsaNI GTAC 3 cut(s) 18, 26, 2242
RseI CAYNNNNRTG 2 cut(s) 11, 681
SaqAI TTAA 4 cut(s) 57, 549, 1239, 2022
Sau3AI GATC 7 cut(s) 156, 1053, 1081, 1454, 1751, 1814, 2125
Sau96I GGNCC 4 cut(s) 742, 1016, 1547, 1784
ScaI AGTACT 1 cut(s) 27
SchI GAGTC 3 cut(s) 327, 479, 1196
ScrFI CCNGG 5 cut(s) 979, 1130, 1596, 1886, 1887
SduI GDGCHC 3 cut(s) 589, 775, 2098
SexAI ACCWGGT 1 cut(s) 1594
SfaNI GCATC 6 cut(s) 15, 661, 1309, 1633, 1732, 1886
SfcI CTRYAG 5 cut(s) 705, 972, 1206, 1212, 1387
SinI GGWCC 3 cut(s) 1016, 1547, 1784
SmaI CCCGGG 1 cut(s) 1887
SmiMI CAYNNNNRTG 2 cut(s) 11, 681
SmlI CTYRAG 3 cut(s) 279, 1154, 1574
SmoI CTYRAG 3 cut(s) 279, 1154, 1574
SpeI ACTAGT 1 cut(s) 350
SphI GCATGC 1 cut(s) 2282
SsiI CCGC 5 cut(s) 275, 514, 1338, 1545, 1640
SspI AATATT 1 cut(s) 403
SspMI CTAG 4 cut(s) 351, 699, 783, 1760
StyD4I CCNGG 5 cut(s) 977, 1128, 1594, 1884, 1885
StyI CCWWGG 7 cut(s) 682, 745, 1049, 1077, 1248, 1521, 1938
TaiI ACGT 1 cut(s) 592
TaqI TCGA 8 cut(s) 74, 316, 641, 1084, 1673, 1750, 1842, 2202
TaqII GACCGA 1 cut(s) 1277
TatI WGTACW 2 cut(s) 17, 25
TauI GCSGC 1 cut(s) 278
TfiI GAWTC 4 cut(s) 164, 1226, 1363, 1631
Tru1I TTAA 4 cut(s) 57, 549, 1239, 2022
Tru9I TTAA 4 cut(s) 57, 549, 1239, 2022
TscAI CASTG 7 cut(s) 87, 166, 700, 709, 1725, 2148, 2307
TseFI GTSAC 4 cut(s) 721, 891, 1762, 2080
Tsp45I GTSAC 4 cut(s) 721, 891, 1762, 2080
TspGWI ACGGA 1 cut(s) 2095
TspMI CCCGGG 1 cut(s) 1885
TspRI CASTG 7 cut(s) 87, 166, 700, 709, 1725, 2148, 2307
VpaK11BI GGWCC 3 cut(s) 1016, 1547, 1784
XapI RAATTY 6 cut(s) 290, 653, 948, 2056, 2289, 2311
XceI RCATGY 3 cut(s) 1924, 1992, 2282
XmaI CCCGGG 1 cut(s) 1885
XmiI GTMKAC 2 cut(s) 228, 1679
XspI CTAG 4 cut(s) 351, 699, 783, 1760
ZrmI AGTACT 1 cut(s) 27
Zsp2I ATGCAT 1 cut(s) 1926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.