Prupe.5G133700_v2.0.a1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0001127
Physical Location & Seq
Forward (+)
12788296 .. 12791745
3450 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G133700.1

Sequence Viewer

Length: 2175 bp
AGAGAGAGATATAATGGGTTTCCTTTGGGTGAAACTGAGAGCAACAAGGCTTTACTATTTGTGGCAGCAAGTCTTTTGTTAGTTTTCAATGGCCAGAACACCCTGATTGCACCGGTTGATGCCGAGAGTAGACTTCACATAGTTTATATTGGGGAGAAGCAGCATGAGGATCCTGAGCTTGTAACCGGTTCACATCATGAGATGCTGGCATCAGTTGTTGGGAGCAAAGAAGCCGCCATTGACTCAATAGTCTATAGCTATAAACATGGCTTCTCTGGTTTCGCAGCCAAGCTTACAGAATCTGAAGCCAAGGAGATTGCAGAGTTACCCGGAGTTGCTCATGTCATGCCTAATAGCTTTCATACATTACAAAATACAAGGAGTTGGGATTATCTAGGCCTCTCTTCTCATTCCCCCACCAATCTTATGCATGACACAAAGATGGGAGATGGGATTAGTCAAAACACGGGTCGGTGCGAATCAGGAGAACACTTCAATGCCACAACGGACTGCAACAAAAAACTTCTAGGAGCAAAGTGGTTCATAGATGGTTTCCTTGCTGACAATGAACAACCATTCAATACCACTGAATTCCCAGAGTTCTTGTCTCCTAGAGATGCAATGGGACATGGCACACACACTGCCACAACCGCTGCTGGTTCCTTTGTGGCCAGTGCAAGTTACAAAGGCCTCGGCCTAGGAGTAGTTAGAGGTGGTGCACCCCGGGCTCATTTGGCCGTGTACAAGCCATGTTGGTATGCACCAATTGCAGTGTGTGCTGCGGCTGATATGCTGAAAGCTTTCAATGAGGCTATTTATGATGGGGTTGATGTGTTGTCACTGTCTATGGGAAACTACCTTCCTAAGTTCGCAGAAGTTGATAAGCGTGACGCAATTTCCACAGGCTCATTCCATGCCGTGGCCAAGGGTATTAGTGTTGTTTGTGCTGCAGACAATACTGGACCTTATGCTCAGACAGTAAGGAATGTAGCATCCTGGATCTTAACTGGGCAGGCCATGTTTGTAGGAAAAGAGGTTGGTTTTACTGGTCTGGTGTATCCAGAGGGCCCAGAACTCTTCCCTACAACAGCTGGTGTCTGCGAGTCCCTGTCACTTAATAACACACCTGTGGCCGGAAATGTAGTCCTTTGCTTCACAACAGTGCCCGGGCCAGCACAAGCAATAAGCGCTGTATCCGCTGTGAGAAGTAAGGGTGGGGTTAGGGTGATTATTGCAAGGCATCCTAGTAATCTCTTGGCTCCATTCAGCAATGACTTCCCATGCATTGCGGTAGACCATGAACTTGGCACCCAGATACTGCTTTACATTCGTTCCACCATATCTCCTGTAGTAAAATTGATCCCTTCTAAAACACTGGTGGGGAAGCCTGTATCAACCAAAGTTGCCTACTTCTCTGCTAAAGGGCCTAATTCAATCGCTCCAGCCATTTTGAAGCCGGATATAGCTGCACCCAGTGTGAGTATATTAGCAGCCAGTACTCTCTTTGATCCCGTTATGGATGGAGGATTCGCCTTTCATTCAGGGACATCAATGGCAACTCCTCACATTGCAGGCATTGTGGCACTTTTAAAAGCATTGCATCCATGGAAGACTGATCCATTTGGAGAGCCCATATTTGCTGAAGGAACTTTGCAGAAGCTCGCGGATCCATTTGATTACAGAGGAGGACTAGTGAGCCTGAACAAGGCAGCAGACCCTGGCCTCATATATGACATGGGAACAGATGACTACATAAACTACCTTTGTGCTGTTGGCTACAACATCTCAGCTATCTCTCAACTTGTTGGACAAGCCACAGCATGTCCCATTATTACGCCTTCCATTCTGGATGTGAACCTACCTTCCATTACCATTCCAAATCTAAGAAAGTCGATAACACTCACTCGAAGTGTGACAAATGTTGGTCCTGTCAACTCAATATATAAAGCTCAAATAGACCCTCCACCAGGCATTAAAGTAAGTGTAAAACCAGATACATTGGTTTTCAATTCCACAGTTAAGACAAACTCCTTCATGGTAGCAGTTTCCACCACCTACCAAGTGAACACAGCATACTTTTTTGGAAGCCTGACTTGGAGTGATGGGGTGCATGATGTGACAAGTCCCATATCAGTGAGAACACAGATCATACAATCCTATACTGACGACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

725

Amino Acids

77.06

Weight (kDa)

5.99

Isoelectric Point (pI)

28.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 248
AccB1I GGYRCC 1 cut(s) 1307
AccB7I CCANNNNNTGG 1 cut(s) 919
AccI GTMKAC 2 cut(s) 130, 1293
AccII CGCG 1 cut(s) 1664
AciI CCGC 6 cut(s) 234, 651, 782, 1197, 1289, 1664
AclWI GGATC 8 cut(s) 164, 177, 1007, 1354, 1502, 1610, 1661, 1674
AcoI YGGCCR 5 cut(s) 91, 669, 735, 921, 1131
AcsI RAATTY 1 cut(s) 590
AcuI CTGAAG 2 cut(s) 324, 1662
AdeI CACNNNGTG 1 cut(s) 1475
AfaI GTAC 2 cut(s) 743, 1498
AfeI AGCGCT 1 cut(s) 1189
AfiI CCNNNNNNNGG 4 cut(s) 919, 1133, 1705, 1967
AgeI ACCGGT 2 cut(s) 112, 185
AgsI TTSAA 7 cut(s) 88, 496, 580, 805, 1434, 1453, 2008
AhlI ACTAGT 1 cut(s) 1690
AjnI CCWGG 3 cut(s) 995, 1717, 1966
AjuI GAANNNNNNNTTGG 2 cut(s) 2077, 2109
AleI CACNNNNGTG 2 cut(s) 1127, 1160
AloI GAACNNNNNNTCC 2 cut(s) 1847, 1879
Alw21I GWGCWC 1 cut(s) 721
Alw26I GTCTC 1 cut(s) 612
Alw44I GTGCAC 1 cut(s) 717
AlwI GGATC 8 cut(s) 164, 177, 1007, 1354, 1502, 1610, 1661, 1674
AlwNI CAGNNNCTG 3 cut(s) 302, 1318, 1718
Ama87I CYCGRG 2 cut(s) 723, 1166
Aor51HI AGCGCT 1 cut(s) 1189
ApaI GGGCCC 1 cut(s) 1070
ApaLI GTGCAC 1 cut(s) 717
ApeKI GCWGC 9 cut(s) 65, 160, 284, 653, 779, 947, 1466, 1490, 1709
ApoI RAATTY 1 cut(s) 590
ArsI GACNNNNNNTTYG 2 cut(s) 454, 486
AsiGI ACCGGT 2 cut(s) 112, 185
Asp700I GAANNNNTTC 1 cut(s) 800
AspA2I CCTAGG 1 cut(s) 697
AspLEI GCGC 1 cut(s) 1190
AspS9I GGNCC 6 cut(s) 962, 1066, 1067, 1169, 1424, 1925
AsuC2I CCSGG 5 cut(s) 330, 724, 725, 1167, 1168
AsuHPI GGTGA 2 cut(s) 41, 1237
AvaI CYCGRG 2 cut(s) 723, 1166
AvaII GGWCC 2 cut(s) 962, 1925
AvrII CCTAGG 1 cut(s) 697
BaeGI GKGCMC 3 cut(s) 721, 1070, 1167
BaeI ACNNNNGTAYC 2 cut(s) 1986, 2019
BalI TGGCCA 3 cut(s) 93, 671, 923
BamHI GGATCC 2 cut(s) 169, 1666
BanI GGYRCC 1 cut(s) 1307
BanII GRGCYC 3 cut(s) 730, 1070, 1632
BbsI GAAGAC 1 cut(s) 1616
Bbv12I GWGCWC 1 cut(s) 721
BbvI GCAGC 9 cut(s) 77, 172, 296, 640, 766, 934, 1453, 1502, 1721
BccI CCATC 6 cut(s) 436, 443, 542, 815, 1514, 2096
BceAI ACGGC 2 cut(s) 722, 902
BciT130I CCWGG 3 cut(s) 997, 1719, 1968
BciVI GTATCC 2 cut(s) 1068, 1204
BcnI CCSGG 5 cut(s) 330, 724, 725, 1167, 1168
BcoDI GTCTC 1 cut(s) 612
BcuI ACTAGT 1 cut(s) 1690
BfaI CTAG 6 cut(s) 395, 527, 612, 698, 1245, 1691
BfmI CTRYAG 3 cut(s) 253, 948, 1347
BfoI RGCGCY 1 cut(s) 1191
BfuI GTATCC 2 cut(s) 1068, 1204
BlnI CCTAGG 1 cut(s) 697
BmcAI AGTACT 1 cut(s) 1498
Bme1390I CCNGG 8 cut(s) 330, 724, 725, 997, 1167, 1168, 1719, 1968
Bme18I GGWCC 2 cut(s) 962, 1925
BmeT110I CYCGRG 2 cut(s) 723, 1166
BmgT120I GGNCC 6 cut(s) 962, 1066, 1067, 1169, 1424, 1925
BmiI GGNNCC 6 cut(s) 171, 661, 1068, 1260, 1309, 1668
BmrFI CCNGG 8 cut(s) 330, 724, 725, 997, 1167, 1168, 1719, 1968
BmrI ACTGGG 2 cut(s) 1017, 1467
BmsI GCATC 7 cut(s) 109, 192, 218, 607, 1001, 1249, 1609
BmuI ACTGGG 2 cut(s) 1017, 1467
BpiI GAAGAC 1 cut(s) 1616
BpmI CTGGAG 1 cut(s) 1425
Bpu10I CCTNAGC 1 cut(s) 174
BpuMI CCSGG 5 cut(s) 330, 724, 725, 1167, 1168
BsaWI WCCGGW 2 cut(s) 112, 185
BsaXI ACNNNNNCTCC 4 cut(s) 1327, 1357, 1677, 1707
Bsc4I CCNNNNNNNGG 4 cut(s) 919, 1133, 1705, 1967
Bse118I RCCGGY 2 cut(s) 112, 185
Bse1I ACTGG 7 cut(s) 672, 964, 1012, 1051, 1380, 1473, 1494
Bse3DI GCAATG 5 cut(s) 627, 1276, 1284, 1566, 1595
BseBI CCWGG 3 cut(s) 997, 1719, 1968
BseGI GGATG 5 cut(s) 992, 1240, 1525, 1600, 1855
BseLI CCNNNNNNNGG 4 cut(s) 919, 1133, 1705, 1967
BseMI GCAATG 5 cut(s) 627, 1276, 1284, 1566, 1595
BseMII CTCAG 4 cut(s) 27, 165, 986, 1800
BseNI ACTGG 7 cut(s) 672, 964, 1012, 1051, 1380, 1473, 1494
BseRI GAGGAG 2 cut(s) 1551, 1698
BseSI GKGCMC 3 cut(s) 721, 1070, 1167
BseXI GCAGC 9 cut(s) 77, 172, 296, 640, 766, 934, 1453, 1502, 1721
BsgI GTGCAG 1 cut(s) 1452
Bsh1236I CGCG 1 cut(s) 1664
BshNI GGYRCC 1 cut(s) 1307
BshTI ACCGGT 2 cut(s) 112, 185
BsiHKAI GWGCWC 1 cut(s) 721
BsiHKCI CYCGRG 2 cut(s) 723, 1166
BsiSI CCGG 7 cut(s) 113, 186, 330, 724, 1134, 1167, 1457
BslFI GGGAC 5 cut(s) 639, 1090, 1558, 1809, 2109
BslI CCNNNNNNNGG 4 cut(s) 919, 1133, 1705, 1967
BsmAI GTCTC 1 cut(s) 612
BsmFI GGGAC 5 cut(s) 639, 1090, 1558, 1809, 2109
BsoBI CYCGRG 2 cut(s) 723, 1166
Bsp120I GGGCCC 1 cut(s) 1066
Bsp1286I GDGCHC 5 cut(s) 721, 730, 1070, 1167, 1632
Bsp1407I TGTACA 1 cut(s) 741
Bsp143I GATC 7 cut(s) 169, 999, 1359, 1507, 1615, 1666, 2145
Bsp19I CCATGG 1 cut(s) 1604
BspACI CCGC 6 cut(s) 234, 651, 782, 1197, 1289, 1664
BspCNI CTCAG 4 cut(s) 28, 166, 985, 1799
BspFNI CGCG 1 cut(s) 1664
BspHI TCATGA 1 cut(s) 196
BspLI GGNNCC 6 cut(s) 171, 661, 1068, 1260, 1309, 1668
BspMAI CTGCAG 1 cut(s) 952
BspPI GGATC 8 cut(s) 164, 177, 1007, 1354, 1502, 1610, 1661, 1674
BspT107I GGYRCC 1 cut(s) 1307
BsrDI GCAATG 5 cut(s) 627, 1276, 1284, 1566, 1595
BsrFI RCCGGY 2 cut(s) 112, 185
BsrGI TGTACA 1 cut(s) 741
BsrI ACTGG 7 cut(s) 672, 964, 1012, 1051, 1380, 1473, 1494
BssAI RCCGGY 2 cut(s) 112, 185
BssMI GATC 7 cut(s) 169, 999, 1359, 1507, 1615, 1666, 2145
BssT1I CCWWGG 4 cut(s) 309, 697, 924, 1604
Bst2UI CCWGG 3 cut(s) 997, 1719, 1968
Bst4CI ACNGT 4 cut(s) 843, 979, 1162, 2017
Bst6I CTCTTC 2 cut(s) 409, 1082
BstAPI GCANNNNNTGC 2 cut(s) 767, 776
BstAUI TGTACA 1 cut(s) 741
BstC8I GCNNGC 5 cut(s) 207, 1014, 1173, 1573, 1662
BstDEI CTNAG 6 cut(s) 36, 174, 864, 972, 1786, 1883
BstDSI CCRYGG 2 cut(s) 918, 1604
BstENI CCTNNNNNAGG 2 cut(s) 1703, 1965
BstF5I GGATG 5 cut(s) 992, 1240, 1525, 1600, 1855
BstFNI CGCG 1 cut(s) 1664
BstH2I RGCGCY 1 cut(s) 1191
BstHHI GCGC 1 cut(s) 1190
BstKTI GATC 7 cut(s) 172, 1002, 1362, 1510, 1618, 1669, 2148
BstMAI GTCTC 1 cut(s) 612
BstMBI GATC 7 cut(s) 169, 999, 1359, 1507, 1615, 1666, 2145
BstMWI GCNNNNNNNGC 7 cut(s) 650, 725, 734, 767, 776, 1187, 1196
BstNI CCWGG 3 cut(s) 997, 1719, 1968
BstNSI RCATGY 1 cut(s) 1824
BstSCI CCNGG 8 cut(s) 328, 722, 723, 995, 1165, 1166, 1717, 1966
BstSFI CTRYAG 3 cut(s) 253, 948, 1347
BstSLI GKGCMC 3 cut(s) 721, 1070, 1167
BstUI CGCG 1 cut(s) 1664
BstV1I GCAGC 9 cut(s) 77, 172, 296, 640, 766, 934, 1453, 1502, 1721
BstV2I GAAGAC 1 cut(s) 1616
BstX2I RGATCY 3 cut(s) 169, 999, 1666
BstXI CCANNNNNNTGG 1 cut(s) 1304
BstYI RGATCY 3 cut(s) 169, 999, 1666
BsuI GTATCC 2 cut(s) 1068, 1204
BtgI CCRYGG 2 cut(s) 918, 1604
BtsCI GGATG 5 cut(s) 992, 1240, 1525, 1600, 1855
BtsI GCAGTG 2 cut(s) 639, 777
BtsIMutI CAGTG 9 cut(s) 585, 639, 679, 777, 839, 1167, 1373, 1480, 2139
Cac8I GCNNGC 5 cut(s) 207, 1014, 1173, 1573, 1662
CaiI CAGNNNCTG 3 cut(s) 302, 1318, 1718
CciI TCATGA 1 cut(s) 196
CfoI GCGC 1 cut(s) 1190
Cfr10I RCCGGY 2 cut(s) 112, 185
Cfr13I GGNCC 6 cut(s) 962, 1066, 1067, 1169, 1424, 1925
Cfr9I CCCGGG 2 cut(s) 723, 1166
CseI GACGC 1 cut(s) 899
Csp6I GTAC 2 cut(s) 742, 1497
CspAI ACCGGT 2 cut(s) 112, 185
CviQI GTAC 2 cut(s) 742, 1497
DdeI CTNAG 6 cut(s) 36, 174, 864, 972, 1786, 1883
DpnI GATC 7 cut(s) 171, 1001, 1361, 1509, 1617, 1668, 2147
DpnII GATC 7 cut(s) 169, 999, 1359, 1507, 1615, 1666, 2145
DraI TTTAAA 1 cut(s) 1590
DraIII CACNNNGTG 1 cut(s) 1475
DrdI GACNNNNNNGTC 1 cut(s) 248
DseDI GACNNNNNNGTC 1 cut(s) 248
EaeI YGGCCR 5 cut(s) 91, 669, 735, 921, 1131
Eam1104I CTCTTC 2 cut(s) 409, 1082
EarI CTCTTC 2 cut(s) 409, 1082
Eco130I CCWWGG 4 cut(s) 309, 697, 924, 1604
Eco147I AGGCCT 2 cut(s) 399, 690
Eco24I GRGCYC 3 cut(s) 730, 1070, 1632
Eco47I GGWCC 2 cut(s) 962, 1925
Eco47III AGCGCT 1 cut(s) 1189
Eco57I CTGAAG 2 cut(s) 324, 1662
Eco88I CYCGRG 2 cut(s) 723, 1166
EcoNI CCTNNNNNAGG 2 cut(s) 1703, 1965
EcoO109I RGGNCCY 2 cut(s) 1066, 1424
EcoRI GAATTC 1 cut(s) 590
EcoRII CCWGG 3 cut(s) 995, 1717, 1966
EcoT14I CCWWGG 4 cut(s) 309, 697, 924, 1604
EcoT22I ATGCAT 2 cut(s) 432, 1286
EcoT38I GRGCYC 3 cut(s) 730, 1070, 1632
ErhI CCWWGG 4 cut(s) 309, 697, 924, 1604
FalI AAGNNNNNCTT 2 cut(s) 2077, 2109
FaqI GGGAC 5 cut(s) 639, 1090, 1558, 1809, 2109
FblI GTMKAC 2 cut(s) 130, 1293
FokI GGATG 5 cut(s) 979, 1227, 1532, 1587, 1862
FriOI GRGCYC 3 cut(s) 730, 1070, 1632
FspBI CTAG 6 cut(s) 395, 527, 612, 698, 1245, 1691
GlaI GCGC 1 cut(s) 1189
GsuI CTGGAG 1 cut(s) 1425
HaeII RGCGCY 1 cut(s) 1191
HapII CCGG 7 cut(s) 113, 186, 330, 724, 1134, 1167, 1457
HgaI GACGC 1 cut(s) 899
HhaI GCGC 1 cut(s) 1190
Hin6I GCGC 1 cut(s) 1188
HinP1I GCGC 1 cut(s) 1188
HincII GTYRAC 1 cut(s) 1933
HindII GTYRAC 1 cut(s) 1933
HindIII AAGCTT 2 cut(s) 290, 798
HinfI GANTC 5 cut(s) 242, 299, 479, 1103, 1527
HpaII CCGG 7 cut(s) 113, 186, 330, 724, 1134, 1167, 1457
HphI GGTGA 2 cut(s) 41, 1237
Hpy166II GTNNAC 8 cut(s) 131, 191, 719, 742, 1294, 1855, 1933, 2065
Hpy188I TCNGA 2 cut(s) 304, 975
Hpy188III TCNNGA 5 cut(s) 173, 197, 483, 1061, 1847
Hpy8I GTNNAC 8 cut(s) 131, 191, 719, 742, 1294, 1855, 1933, 2065
HpyAV CCTTC 6 cut(s) 869, 1374, 1637, 1848, 1872, 2041
HpyCH4III ACNGT 4 cut(s) 843, 979, 1162, 2017
HpyF10VI GCNNNNNNNGC 7 cut(s) 650, 725, 734, 767, 776, 1187, 1196
HpyF3I CTNAG 6 cut(s) 36, 174, 864, 972, 1786, 1883
HspAI GCGC 1 cut(s) 1188
Kzo9I GATC 7 cut(s) 169, 999, 1359, 1507, 1615, 1666, 2145
LmnI GCTCC 4 cut(s) 222, 530, 1264, 1444
Lsp1109I GCAGC 9 cut(s) 77, 172, 296, 640, 766, 934, 1453, 1502, 1721
LweI GCATC 7 cut(s) 109, 192, 218, 607, 1001, 1249, 1609
MaeI CTAG 6 cut(s) 395, 527, 612, 698, 1245, 1691
MaeIII GTNAC 8 cut(s) 181, 324, 680, 837, 887, 1110, 1912, 2116
MalI GATC 7 cut(s) 171, 1001, 1361, 1509, 1617, 1668, 2147
MboI GATC 7 cut(s) 169, 999, 1359, 1507, 1615, 1666, 2145
MboII GAAGA 3 cut(s) 396, 1069, 1621
MfeI CAATTG 2 cut(s) 765, 2170
MflI RGATCY 3 cut(s) 169, 999, 1666
MhlI GDGCHC 5 cut(s) 721, 730, 1070, 1167, 1632
MlsI TGGCCA 3 cut(s) 93, 671, 923
MluCI AATT 7 cut(s) 590, 765, 894, 1355, 1429, 2008, 2170
MluNI TGGCCA 3 cut(s) 93, 671, 923
MlyI GAGTC 2 cut(s) 236, 1112
MmeI TCCRAC 1 cut(s) 1786
Mox20I TGGCCA 3 cut(s) 93, 671, 923
Mph1103I ATGCAT 2 cut(s) 432, 1286
MroXI GAANNNNTTC 1 cut(s) 800
MscI TGGCCA 3 cut(s) 93, 671, 923
MseI TTAA 5 cut(s) 1004, 1116, 1589, 1974, 2019
MslI CAYNNNNRTG 5 cut(s) 440, 495, 1127, 1160, 2132
Msp20I TGGCCA 3 cut(s) 93, 671, 923
MspA1I CMGCKG 3 cut(s) 653, 1091, 1199
MspI CCGG 7 cut(s) 113, 186, 330, 724, 1134, 1167, 1457
MspR9I CCNGG 8 cut(s) 330, 724, 725, 997, 1167, 1168, 1719, 1968
MunI CAATTG 2 cut(s) 765, 2170
MvaI CCWGG 3 cut(s) 997, 1719, 1968
MvnI CGCG 1 cut(s) 1664
MwoI GCNNNNNNNGC 7 cut(s) 650, 725, 734, 767, 776, 1187, 1196
NciI CCSGG 5 cut(s) 330, 724, 725, 1167, 1168
NcoI CCATGG 1 cut(s) 1604
NdeII GATC 7 cut(s) 169, 999, 1359, 1507, 1615, 1666, 2145
NlaIV GGNNCC 6 cut(s) 171, 661, 1068, 1260, 1309, 1668
NmeAIII GCCGAG 2 cut(s) 148, 672
NmuCI GTSAC 5 cut(s) 837, 887, 1110, 1912, 2116
NsiI ATGCAT 2 cut(s) 432, 1286
NspI RCATGY 1 cut(s) 1824
OliI CACNNNNGTG 2 cut(s) 1127, 1160
PagI TCATGA 1 cut(s) 196
PceI AGGCCT 2 cut(s) 399, 690
PdmI GAANNNNTTC 1 cut(s) 800
PfeI GAWTC 3 cut(s) 299, 479, 1527
PflMI CCANNNNNTGG 1 cut(s) 919
PfoI TCCNGGA 1 cut(s) 995
PinAI ACCGGT 2 cut(s) 112, 185
PleI GAGTC 2 cut(s) 236, 1111
PpsI GAGTC 2 cut(s) 236, 1111
Psp6I CCWGG 3 cut(s) 995, 1717, 1966
PspGI CCWGG 3 cut(s) 995, 1717, 1966
PspN4I GGNNCC 6 cut(s) 171, 661, 1068, 1260, 1309, 1668
PspOMI GGGCCC 1 cut(s) 1066
PspPI GGNCC 6 cut(s) 962, 1066, 1067, 1169, 1424, 1925
PsrI GAACNNNNNNTAC 2 cut(s) 2057, 2089
PstI CTGCAG 1 cut(s) 952
PstNI CAGNNNCTG 3 cut(s) 302, 1318, 1718
PsuI RGATCY 3 cut(s) 169, 999, 1666
PvuII CAGCTG 1 cut(s) 1091
RsaI GTAC 2 cut(s) 743, 1498
RsaNI GTAC 2 cut(s) 742, 1497
RseI CAYNNNNRTG 5 cut(s) 440, 495, 1127, 1160, 2132
SaqAI TTAA 5 cut(s) 1004, 1116, 1589, 1974, 2019
Sau3AI GATC 7 cut(s) 169, 999, 1359, 1507, 1615, 1666, 2145
Sau96I GGNCC 6 cut(s) 962, 1066, 1067, 1169, 1424, 1925
ScaI AGTACT 1 cut(s) 1498
SchI GAGTC 2 cut(s) 236, 1112
ScrFI CCNGG 8 cut(s) 330, 724, 725, 997, 1167, 1168, 1719, 1968
SduI GDGCHC 5 cut(s) 721, 730, 1070, 1167, 1632
SfaNI GCATC 7 cut(s) 109, 192, 218, 607, 1001, 1249, 1609
SfcI CTRYAG 3 cut(s) 253, 948, 1347
SinI GGWCC 2 cut(s) 962, 1925
SmaI CCCGGG 2 cut(s) 725, 1168
SmiMI CAYNNNNRTG 5 cut(s) 440, 495, 1127, 1160, 2132
SpeI ACTAGT 1 cut(s) 1690
SrfI GCCCGGGC 1 cut(s) 1168
Sse9I AATT 7 cut(s) 590, 765, 894, 1355, 1429, 2008, 2170
SseBI AGGCCT 2 cut(s) 399, 690
SsiI CCGC 6 cut(s) 234, 651, 782, 1197, 1289, 1664
SspMI CTAG 6 cut(s) 395, 527, 612, 698, 1245, 1691
StuI AGGCCT 2 cut(s) 399, 690
StyD4I CCNGG 8 cut(s) 328, 722, 723, 995, 1165, 1166, 1717, 1966
StyI CCWWGG 4 cut(s) 309, 697, 924, 1604
TaaI ACNGT 4 cut(s) 843, 979, 1162, 2017
TaqI TCGA 2 cut(s) 1892, 1906
TasI AATT 7 cut(s) 590, 765, 894, 1355, 1429, 2008, 2170
TatI WGTACW 2 cut(s) 741, 1496
TauI GCSGC 2 cut(s) 236, 785
TfiI GAWTC 3 cut(s) 299, 479, 1527
Tru1I TTAA 5 cut(s) 1004, 1116, 1589, 1974, 2019
Tru9I TTAA 5 cut(s) 1004, 1116, 1589, 1974, 2019
TscAI CASTG 9 cut(s) 592, 646, 679, 777, 846, 1167, 1380, 1480, 2139
TseFI GTSAC 5 cut(s) 837, 887, 1110, 1912, 2116
TseI GCWGC 9 cut(s) 65, 160, 284, 653, 779, 947, 1466, 1490, 1709
Tsp45I GTSAC 5 cut(s) 837, 887, 1110, 1912, 2116
TspDTI ATGAA 6 cut(s) 350, 532, 582, 1314, 1526, 2023
TspGWI ACGGA 1 cut(s) 521
TspMI CCCGGG 2 cut(s) 723, 1166
TspRI CASTG 9 cut(s) 592, 646, 679, 777, 846, 1167, 1380, 1480, 2139
Van91I CCANNNNNTGG 1 cut(s) 919
VneI GTGCAC 1 cut(s) 717
VpaK11BI GGWCC 2 cut(s) 962, 1925
XagI CCTNNNNNAGG 2 cut(s) 1703, 1965
XapI RAATTY 1 cut(s) 590
XceI RCATGY 1 cut(s) 1824
XmaI CCCGGG 2 cut(s) 723, 1166
XmaJI CCTAGG 1 cut(s) 697
XmiI GTMKAC 2 cut(s) 130, 1293
XmnI GAANNNNTTC 1 cut(s) 800
XspI CTAG 6 cut(s) 395, 527, 612, 698, 1245, 1691
ZrmI AGTACT 1 cut(s) 1498
Zsp2I ATGCAT 2 cut(s) 432, 1286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.