Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0001127

Physical Location & Seq

Genomic Coordinates Forward (+)

6499794 .. 6502933

Gene Structure 3140 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G10520.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2271 bp

ATGAGCAAAACCATACTTTTCTTGGCGTTATTTCTATCTATAGTTTTGAATGTGCAGATCAGTTTCGTCGTAGCTGAGAGCAAGGTTTATGTAGTTTACTTGGGAGAGAAGGAACATGACAATCCTGAGTCTGTCACTGAATCTCACCATCAGATGTTGTGGTCACTTCTTGGAAGCAAAGAGGCTGTCCTTGATTCAATAGTGTATAGTTATCGACACGGCTTTTCAGGCTTTGCAGCCAAGCTCACTGAGTCCCAAGCACAGCAAATTTCTGAACTGCCTGAAGTAGTACAAGTCATACCAAATACATTATACGAAATGACAACAACAAGAACTTGGGATTACTTAGGTGTATCTCCGGGAAATTCAGATAGTCTCCTACAAAAGGCCAATATGGGGTACAATGTAATCGTTGGAGTCATCGACTCAGGAGTGTGGCCAGAGTCTGAAATGTTTAATGACAAAGGCTTTGGACCTATACCAAGTCGTTGGAAAGGCGGCTGTGAATCTGGAGAACTCTTCAACGCCTCGATTCATTGCAACAGAAAGCTGATAGGAGCAAAATACTTTGTTGATGGTCTTGTTGCCGAATTTGGAGTCGTGAACAGAACTCAGAACCCTGAGTATCTTTCTCCCAGAGACTTTGCCGGTCATGGCACACATGTCGCCTCGACCATTGGTGGTTCTTTTCTGCCTAATGTAAGCTACGTAGGCCTCGGACGAGGAACTGCAAGAGGCGGTGCTCCCGGTGTTCATATAGCTGTTTATAAGGCATGCTGGAGTGGGTATTGTTCGGGAGCTGATGTATTAAAAGCAATGGATGAAGCTATCCATGATGGTGTTGATATTTTGTCACTTTCTTTGGGACCTAGTGTTCCTTTATTTCCTGAAACGGAGCATACCTCCGTGGGAGCATTCCATGCGGTCGCAAAGGGAATTCCTGTTGTAATTGCAGCTGGTAATGCTGGCCCCACGGCTCAGACCATTTCGAATGTAGCTCCTTGGGTCTTGACAGTGGCTGCAACCACTCAAGACCGTTCTTTTCCTACAGCCATAACACTTGGGAACAATATAACAATACTGGGTCAAGCAATATACGGCGGTCCAGAACTCGGTTTCGTTGGTCTTACTTATCCAGAGAGTCCACTTTCAGGTGACTGCGAGAAGCTCTCTGCTAATCCCAACAGTACAATGGAAGGAAAAGTTGTGTTGTGTTTCGCAGCATCTACACCATCAAATGCTGCTATAGCCGCAGTAATAAATGCTGGTGGTCTTGGGTTAATCATGGCAAAAAATCCGACCCACTCGCTTACTCCAACTCGTAAGTTTCCCTGGGTTTCCATAGATTTCGAGCTAGGGACCGACATTTTGTTCTATATACGCTCCACAAGATCTCCCATTGTAAAGATACAGGCTTCAAAGACGCTTTTTGGACAATCCGTGTCGACAAAGGTAGCAACCTTCTCGTCAAGAGGACCTAATTCAGTTTCTCCGGCGATTCTTAAGCCGGATATAGCAGCACCTGGTGTGAACATACTAGCAGCTATTTCTCCAAATAGCTCTATAAATGATGGAGGTTTTGCTATGATGTCAGGGACATCAATGGCTACTCCTGTTGTTTCAGGAGTTGTAGTGCTCCTCAAATCATTACATCCTGATTGGTCTCCTTCTGCTATCAAATCAGCTATTGTTACCACAGCTTGGAGAACTGATCCATCAGGAGAGCCAATATTCGCAGACGGGTCAAGCCGCAAGCTAGCTGATCCGTTTGATTACGGAGGAGGACTCATAAATCCAGAGAAAGCTGTAAAACCAGGTCTCATATACGACATGACAACAGATGACTACGTTATGTACATGTGCTCTGTTGATTACAGTGACATTTCCATTTCTCGAGTCCTCGGAAAAATAACTGTCTGTCCTAATCCCAAACCTTCTGTTCTTGATCTAAATTTGCCTTCCATCACAATTCCAAACCTTAGAGGCGAAGTCACTCTCACAAGAACCGTCACTAACGTTGGACCCGTTAACTCGGTGTACAAGGTTGTAATCGATCCTCCAACAGGCATTAACGTGGCTGTTACGCCAGCGGAACTAGTGTTCGACTATACAACTACAAAGCGATCTTTCACTGTTCGGGTCTCGACTACACACAAAGTAAACACTGGTTACTATTTTGGAAGCTTGACTTGGACTGATAATATGCACAATGTAGCCATCCCAGTTTCTGTAAGAACACAAATCTTGCAACGATACTACGACGAAAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009505 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009682 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0016043 GO:0016049 GO:0016787 GO:0017171 GO:0019538 GO:0022414 GO:0030154 GO:0030312 GO:0031012 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0042742 GO:0043170 GO:0043207 GO:0044238 GO:0044421 GO:0044464 GO:0044706 GO:0045087 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0050896 GO:0051704 GO:0051707 GO:0060560 GO:0070011 GO:0071704 GO:0071840 GO:0071944 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

756

Amino Acids

80.67

Weight (kDa)

5.95

Isoelectric Point (pI)

33.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	29 - 107	2.9e-22	Peptidase inhibitor I9
Peptidase_S8	PF00082	134 - 572	1.1e-49	Subtilase family
fn3_6	PF17766	649 - 744	7.8e-29	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001127)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G32940 AT1G32940 AT1G32940 AT1G32940 AT1G32950 AT1G32950 AT1G32960 AT4G10510 AT4G10510 AT4G10510 AT4G10510 AT4G10520 AT4G10530 AT4G10540 AT4G10550 AT4G10550 AT4G10550 AT4G10550
fragaria_vesca	FvH4_5g10490 FvH4_5g10502
malus_domestica	MD00G1035000.v1.1
prunus_persica	Prupe.5G133500_v2.0.a1 Prupe.5G133500_v2.0.a1 Prupe.5G133500_v2.0.a1 Prupe.5G133500_v2.0.a1 Prupe.5G133500_v2.0.a1 Prupe.5G133600_v2.0.a1 Prupe.5G133700_v2.0.a1
pyrus_communis	pycom01g12030 pycom14g11430 pycom14g11440 pycom14g11450 pycom14g11470 pycom14g11480 pycom14g11520
rosa_chinensis	RchiOBHm_Chr4g0389671 RchiOBHm_Chr7g0187621 RchiOBHm_Chr7g0187651
rosa_laevigata	RLG00000004761 RLG00000004765
rosa_multiflora	Rmu_sc0003144.1_g000021 Rmu_sc0004483.1_g000012 Rmu_ssc0000110.1_g000014
rosa_roxburghii	Rroxscaffold_3G00267240
rosa_rugosa	Rorug06G0483200
rosa_samantha	Rh4AG029600 Rh4BG022700 Rh7AG088100 Rh7AG088500 Rh7BG089400 Rh7BG089900 Rh7BG090200 Rh7CG088700 Rh7CG089100 Rh7DG090400 Rh7DG090700
rosa_wichuraiana	Rw0G002650 Rw4G002200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	768
AccB7I	CCANNNNNTGG	1 cut(s)	1701
AccI	GTMKAC	1 cut(s)	1445
AciI	CCGC	7 cut(s)	498, 738, 923, 1101, 1251, 1750, 2090
AclI	AACGTT	1 cut(s)	2016
AclWI	GGATC	3 cut(s)	1706, 1757, 2048
AcoI	YGGCCR	1 cut(s)	437
AcsI	RAATTY	5 cut(s)	267, 364, 590, 936, 1951
AcuI	CTGAAG	1 cut(s)	303
AdeI	CACNNNGTG	1 cut(s)	1526
AfaI	GTAC	5 cut(s)	291, 401, 1189, 1856, 2039
AfiI	CCNNNNNNNGG	6 cut(s)	385, 396, 1112, 1151, 1701, 2063
AflII	CTTAAG	1 cut(s)	1502
AflIII	ACRYGT	2 cut(s)	661, 1857
AgsI	TTSAA	4 cut(s)	49, 198, 523, 1419
AhlI	ACTAGT	1 cut(s)	2095
AjnI	CCWGG	3 cut(s)	1331, 1522, 1813
AjuI	GAANNNNNNNTTGG	2 cut(s)	2173, 2205
AloI	GAACNNNNNNTCC	4 cut(s)	858, 890, 2084, 2116
Alw21I	GWGCWC	3 cut(s)	745, 1638, 1865
Alw26I	GTCTC	5 cut(s)	380, 633, 1668, 1823, 2146
AlwI	GGATC	3 cut(s)	1706, 1757, 2048
AlwNI	CAGNNNCTG	4 cut(s)	446, 1019, 1523, 2228
Ama87I	CYCGRG	1 cut(s)	1893
AoxI	GGCC	4 cut(s)	387, 437, 712, 967
ApeKI	GCWGC	7 cut(s)	236, 953, 1019, 1220, 1241, 1517, 1541
ApoI	RAATTY	5 cut(s)	267, 364, 590, 936, 1951
ArsI	GACNNNNNNTTYG	8 cut(s)	171, 203, 452, 484, 582, 614, 1351, 1383
AspS9I	GGNCC	7 cut(s)	473, 866, 968, 1103, 1359, 1475, 2021
AsuC2I	CCSGG	2 cut(s)	360, 747
AsuHPI	GGTGA	2 cut(s)	137, 1166
AsuII	TTCGAA	1 cut(s)	989
AsuNHI	GCTAGC	1 cut(s)	1756
AvaI	CYCGRG	1 cut(s)	1893
AvaII	GGWCC	6 cut(s)	473, 866, 1103, 1359, 1475, 2021
BalI	TGGCCA	1 cut(s)	439
Bbv12I	GWGCWC	3 cut(s)	745, 1638, 1865
BbvI	GCAGC	7 cut(s)	248, 965, 1006, 1228, 1232, 1529, 1553
BccI	CCATC	8 cut(s)	156, 569, 830, 1240, 1565, 1723, 1970, 2225
BceAI	ACGGC	3 cut(s)	235, 990, 1114
BcgI	CGANNNNNNTGC	4 cut(s)	646, 680, 1446, 1480
BciT130I	CCWGG	3 cut(s)	1333, 1524, 1815
BcnI	CCSGG	2 cut(s)	360, 747
BcoDI	GTCTC	5 cut(s)	380, 633, 1668, 1823, 2146
BcuI	ACTAGT	1 cut(s)	2095
BfaI	CTAG	5 cut(s)	870, 1355, 1538, 1757, 2096
BfmI	CTRYAG	3 cut(s)	39, 1047, 1245
BfrI	CTTAAG	1 cut(s)	1502
BglII	AGATCT	1 cut(s)	1391
Bme1390I	CCNGG	5 cut(s)	360, 747, 1333, 1524, 1815
Bme18I	GGWCC	6 cut(s)	473, 866, 1103, 1359, 1475, 2021
BmeT110I	CYCGRG	1 cut(s)	1893
BmgT120I	GGNCC	7 cut(s)	473, 866, 968, 1103, 1359, 1475, 2021
BmiI	GGNNCC	4 cut(s)	867, 970, 1360, 2023
BmrFI	CCNGG	5 cut(s)	360, 747, 1333, 1524, 1815
BmrI	ACTGGG	2 cut(s)	1091, 2216
BmsI	GCATC	1 cut(s)	1232
BmtI	GCTAGC	1 cut(s)	1760
BmuI	ACTGGG	2 cut(s)	1091, 2216
BplI	GAGNNNNNCTC	6 cut(s)	887, 919, 1154, 1186, 1770, 1802
BpmI	CTGGAG	2 cut(s)	531, 799
Bpu14I	TTCGAA	1 cut(s)	989
BpuEI	CTTGAG	1 cut(s)	1014
BpuMI	CCSGG	2 cut(s)	360, 747
Bsa29I	ATCGAT	1 cut(s)	2052
BsaAI	YACGTR	1 cut(s)	709
BsaI	GGTCTC	3 cut(s)	1668, 1823, 2146
BsaJI	CCNNGG	7 cut(s)	715, 906, 972, 1001, 1331, 1332, 1900
BsaXI	ACNNNNNCTCC	6 cut(s)	789, 819, 1378, 1408, 1617, 1647
Bsc4I	CCNNNNNNNGG	6 cut(s)	385, 396, 1112, 1151, 1701, 2063
Bse118I	RCCGGY	1 cut(s)	647
Bse1I	ACTGG	3 cut(s)	1086, 2170, 2222
Bse3DI	GCAATG	2 cut(s)	535, 822
BseBI	CCWGG	3 cut(s)	1333, 1524, 1815
BseCI	ATCGAT	1 cut(s)	2052
BseDI	CCNNGG	7 cut(s)	715, 906, 972, 1001, 1331, 1332, 1900
BseGI	GGATG	3 cut(s)	826, 1651, 2217
BseLI	CCNNNNNNNGG	6 cut(s)	385, 396, 1112, 1151, 1701, 2063
BseMI	GCAATG	2 cut(s)	535, 822
BseMII	CTCAG	7 cut(s)	66, 117, 240, 441, 612, 626, 992
BseNI	ACTGG	3 cut(s)	1086, 2170, 2222
BseRI	GAGGAG	2 cut(s)	1628, 1794
BseXI	GCAGC	7 cut(s)	248, 965, 1006, 1228, 1232, 1529, 1553
BsgI	GTGCAG	1 cut(s)	74
Bsh1285I	CGRYCG	1 cut(s)	927
BshFI	GGCC	4 cut(s)	389, 439, 714, 969
BshVI	ATCGAT	1 cut(s)	2052
BsiEI	CGRYCG	1 cut(s)	927
BsiHKAI	GWGCWC	3 cut(s)	745, 1638, 1865
BsiHKCI	CYCGRG	1 cut(s)	1893
BsiSI	CCGG	5 cut(s)	359, 648, 747, 1493, 1508
BslFI	GGGAC	4 cut(s)	238, 879, 1372, 1609
BslI	CCNNNNNNNGG	6 cut(s)	385, 396, 1112, 1151, 1701, 2063
BsmAI	GTCTC	5 cut(s)	380, 633, 1668, 1823, 2146
BsmFI	GGGAC	4 cut(s)	238, 879, 1372, 1609
BsmI	GAATGC	1 cut(s)	914
BsnI	GGCC	4 cut(s)	389, 439, 714, 969
Bso31I	GGTCTC	3 cut(s)	1668, 1823, 2146
BsoBI	CYCGRG	1 cut(s)	1893
Bsp119I	TTCGAA	1 cut(s)	989
Bsp1286I	GDGCHC	3 cut(s)	745, 1638, 1865
Bsp1407I	TGTACA	2 cut(s)	1854, 2037
Bsp143I	GATC	7 cut(s)	57, 1391, 1711, 1762, 1945, 2053, 2123
BspACI	CCGC	7 cut(s)	498, 738, 923, 1101, 1251, 1750, 2090
BspANI	GGCC	4 cut(s)	389, 439, 714, 969
BspCNI	CTCAG	7 cut(s)	67, 118, 241, 440, 613, 625, 991
BspDI	ATCGAT	1 cut(s)	2052
BspLI	GGNNCC	4 cut(s)	867, 970, 1360, 2023
BspOI	GCTAGC	1 cut(s)	1760
BspPI	GGATC	3 cut(s)	1706, 1757, 2048
BspT104I	TTCGAA	1 cut(s)	989
BspTI	CTTAAG	1 cut(s)	1502
BspTNI	GGTCTC	3 cut(s)	1668, 1823, 2146
BsrDI	GCAATG	2 cut(s)	535, 822
BsrFI	RCCGGY	1 cut(s)	647
BsrGI	TGTACA	2 cut(s)	1854, 2037
BsrI	ACTGG	3 cut(s)	1086, 2170, 2222
BssAI	RCCGGY	1 cut(s)	647
BssECI	CCNNGG	7 cut(s)	715, 906, 972, 1001, 1331, 1332, 1900
BssMI	GATC	7 cut(s)	57, 1391, 1711, 1762, 1945, 2053, 2123
BssT1I	CCWWGG	1 cut(s)	1001
Bst2UI	CCWGG	3 cut(s)	1333, 1524, 1815
Bst4CI	ACNGT	7 cut(s)	1015, 1037, 1187, 1877, 1915, 2008, 2134
Bst6I	CTCTTC	1 cut(s)	524
BstAFI	CTTAAG	1 cut(s)	1502
BstAPI	GCANNNNNTGC	1 cut(s)	920
BstAUI	TGTACA	2 cut(s)	1854, 2037
BstBAI	YACGTR	1 cut(s)	709
BstBI	TTCGAA	1 cut(s)	989
BstC8I	GCNNGC	5 cut(s)	775, 967, 1754, 1758, 2088
BstDEI	CTNAG	9 cut(s)	75, 126, 249, 346, 427, 612, 621, 978, 1979
BstDSI	CCRYGG	2 cut(s)	906, 972
BstENI	CCTNNNNNAGG	2 cut(s)	383, 2061
BstF5I	GGATG	3 cut(s)	826, 1651, 2217
BstKTI	GATC	7 cut(s)	60, 1394, 1714, 1765, 1948, 2056, 2126
BstMAI	GTCTC	5 cut(s)	380, 633, 1668, 1823, 2146
BstMBI	GATC	7 cut(s)	57, 1391, 1711, 1762, 1945, 2053, 2123
BstMCI	CGRYCG	1 cut(s)	927
BstMWI	GCNNNNNNNGC	6 cut(s)	228, 711, 920, 962, 1247, 1250
BstNI	CCWGG	3 cut(s)	1333, 1524, 1815
BstNSI	RCATGY	3 cut(s)	665, 777, 1861
BstSCI	CCNGG	5 cut(s)	358, 745, 1331, 1522, 1813
BstSFI	CTRYAG	3 cut(s)	39, 1047, 1245
BstSNI	TACGTA	1 cut(s)	709
BstV1I	GCAGC	7 cut(s)	248, 965, 1006, 1228, 1232, 1529, 1553
BstX2I	RGATCY	1 cut(s)	1391
BstXI	CCANNNNNNTGG	1 cut(s)	489
BstYI	RGATCY	1 cut(s)	1391
Bsu15I	ATCGAT	1 cut(s)	2052
BsuRI	GGCC	4 cut(s)	389, 439, 714, 969
BsuTUI	ATCGAT	1 cut(s)	2052
BtgI	CCRYGG	2 cut(s)	906, 972
BtsCI	GGATG	3 cut(s)	826, 1651, 2217
BtsIMutI	CAGTG	6 cut(s)	135, 246, 1020, 1882, 2130, 2163
Cac8I	GCNNGC	5 cut(s)	775, 967, 1754, 1758, 2088
CaiI	CAGNNNCTG	4 cut(s)	446, 1019, 1523, 2228
Cfr10I	RCCGGY	1 cut(s)	647
Cfr13I	GGNCC	7 cut(s)	473, 866, 968, 1103, 1359, 1475, 2021
ClaI	ATCGAT	1 cut(s)	2052
CseI	GACGC	1 cut(s)	1432
CsiI	ACCWGGT	2 cut(s)	1522, 1813
Csp6I	GTAC	5 cut(s)	290, 400, 1188, 1855, 2038
CviAII	CATG	9 cut(s)	116, 653, 662, 774, 833, 920, 1285, 1831, 1858
CviQI	GTAC	5 cut(s)	290, 400, 1188, 1855, 2038
DdeI	CTNAG	9 cut(s)	75, 126, 249, 346, 427, 612, 621, 978, 1979
DpnI	GATC	7 cut(s)	59, 1393, 1713, 1764, 1947, 2055, 2125
DpnII	GATC	7 cut(s)	57, 1391, 1711, 1762, 1945, 2053, 2123
DraIII	CACNNNGTG	1 cut(s)	1526
EaeI	YGGCCR	1 cut(s)	437
Eam1104I	CTCTTC	1 cut(s)	524
EarI	CTCTTC	1 cut(s)	524
Eco105I	TACGTA	1 cut(s)	709
Eco130I	CCWWGG	1 cut(s)	1001
Eco147I	AGGCCT	1 cut(s)	714
Eco31I	GGTCTC	3 cut(s)	1668, 1823, 2146
Eco47I	GGWCC	6 cut(s)	473, 866, 1103, 1359, 1475, 2021
Eco57I	CTGAAG	1 cut(s)	303
Eco88I	CYCGRG	1 cut(s)	1893
EcoNI	CCTNNNNNAGG	2 cut(s)	383, 2061
EcoO109I	RGGNCCY	2 cut(s)	866, 1475
EcoRI	GAATTC	1 cut(s)	936
EcoRII	CCWGG	3 cut(s)	1331, 1522, 1813
EcoT14I	CCWWGG	1 cut(s)	1001
ErhI	CCWWGG	1 cut(s)	1001
FaeI	CATG	9 cut(s)	119, 656, 665, 777, 836, 923, 1288, 1834, 1861
FalI	AAGNNNNNCTT	2 cut(s)	2173, 2205
FaqI	GGGAC	4 cut(s)	238, 879, 1372, 1609
FatI	CATG	9 cut(s)	115, 652, 661, 773, 832, 919, 1284, 1830, 1857
FblI	GTMKAC	1 cut(s)	1445
FokI	GGATG	3 cut(s)	833, 1638, 2204
FspBI	CTAG	5 cut(s)	870, 1355, 1538, 1757, 2096
GsuI	CTGGAG	2 cut(s)	531, 799
HaeIII	GGCC	4 cut(s)	389, 439, 714, 969
HapII	CCGG	5 cut(s)	359, 648, 747, 1493, 1508
HgaI	GACGC	1 cut(s)	1432
Hin1II	CATG	9 cut(s)	119, 656, 665, 777, 836, 923, 1288, 1834, 1861
HincII	GTYRAC	2 cut(s)	1446, 2029
HindII	GTYRAC	2 cut(s)	1446, 2029
HindIII	AAGCTT	1 cut(s)	2182
HpaI	GTTAAC	1 cut(s)	2029
HpaII	CCGG	5 cut(s)	359, 648, 747, 1493, 1508
HphI	GGTGA	2 cut(s)	137, 1166
Hpy166II	GTNNAC	8 cut(s)	97, 604, 1145, 1446, 1531, 2029, 2038, 2161
Hpy188I	TCNGA	9 cut(s)	153, 274, 370, 448, 615, 719, 981, 1299, 1904
Hpy8I	GTNNAC	8 cut(s)	97, 604, 1145, 1446, 1531, 2029, 2038, 2161
Hpy99I	CGWCG	2 cut(s)	71, 2264
HpyAV	CCTTC	6 cut(s)	103, 1190, 1471, 1677, 1944, 1968
HpyCH4III	ACNGT	7 cut(s)	1015, 1037, 1187, 1877, 1915, 2008, 2134
HpyCH4IV	ACGT	4 cut(s)	708, 1848, 2016, 2073
HpyCH4V	TGCA	8 cut(s)	55, 236, 540, 731, 953, 1022, 2206, 2248
HpyF10VI	GCNNNNNNNGC	6 cut(s)	228, 711, 920, 962, 1247, 1250
HpyF3I	CTNAG	9 cut(s)	75, 126, 249, 346, 427, 612, 621, 978, 1979
HpySE526I	ACGT	4 cut(s)	708, 1848, 2016, 2073
Hsp92II	CATG	9 cut(s)	119, 656, 665, 777, 836, 923, 1288, 1834, 1861
KspAI	GTTAAC	1 cut(s)	2029
Kzo9I	GATC	7 cut(s)	57, 1391, 1711, 1762, 1945, 2053, 2123
LmnI	GCTCC	8 cut(s)	557, 748, 797, 895, 911, 1003, 1388, 1641
Lsp1109I	GCAGC	7 cut(s)	248, 965, 1006, 1228, 1232, 1529, 1553
LweI	GCATC	1 cut(s)	1232
MabI	ACCWGGT	2 cut(s)	1522, 1813
MaeI	CTAG	5 cut(s)	870, 1355, 1538, 1757, 2096
MaeII	ACGT	4 cut(s)	708, 1848, 2016, 2073
MalI	GATC	7 cut(s)	59, 1393, 1713, 1764, 1947, 2055, 2125
MboI	GATC	7 cut(s)	57, 1391, 1711, 1762, 1945, 2053, 2123
MboII	GAAGA	1 cut(s)	511
MflI	RGATCY	1 cut(s)	1391
MhlI	GDGCHC	3 cut(s)	745, 1638, 1865
MlsI	TGGCCA	1 cut(s)	439
MluCI	AATT	8 cut(s)	267, 364, 590, 936, 948, 1480, 1951, 1968
MluNI	TGGCCA	1 cut(s)	439
MlyI	GAGTC	9 cut(s)	137, 260, 419, 426, 452, 606, 1150, 1779, 1905
MmeI	TCCRAC	6 cut(s)	394, 470, 1322, 1340, 1999, 2084
Mox20I	TGGCCA	1 cut(s)	439
MscI	TGGCCA	1 cut(s)	439
MseI	TTAA	6 cut(s)	456, 809, 1280, 1503, 2028, 2070
MslI	CAYNNNNRTG	2 cut(s)	837, 2072
Msp20I	TGGCCA	1 cut(s)	439
MspA1I	CMGCKG	2 cut(s)	956, 2090
MspCI	CTTAAG	1 cut(s)	1502
MspI	CCGG	5 cut(s)	359, 648, 747, 1493, 1508
MspR9I	CCNGG	5 cut(s)	360, 747, 1333, 1524, 1815
Mva1269I	GAATGC	1 cut(s)	914
MvaI	CCWGG	3 cut(s)	1333, 1524, 1815
MwoI	GCNNNNNNNGC	6 cut(s)	228, 711, 920, 962, 1247, 1250
NciI	CCSGG	2 cut(s)	360, 747
NdeII	GATC	7 cut(s)	57, 1391, 1711, 1762, 1945, 2053, 2123
NheI	GCTAGC	1 cut(s)	1756
NlaIII	CATG	9 cut(s)	119, 656, 665, 777, 836, 923, 1288, 1834, 1861
NlaIV	GGNNCC	4 cut(s)	867, 970, 1360, 2023
NmuCI	GTSAC	7 cut(s)	133, 162, 852, 1154, 1877, 1990, 2008
NspI	RCATGY	3 cut(s)	665, 777, 1861
NspV	TTCGAA	1 cut(s)	989
PaeI	GCATGC	1 cut(s)	777
PaeR7I	CTCGAG	1 cut(s)	1893
PasI	CCCWGGG	1 cut(s)	1332
PceI	AGGCCT	1 cut(s)	714
PciI	ACATGT	2 cut(s)	661, 1857
PcsI	WCGNNNNNNNCGW	1 cut(s)	2022
PctI	GAATGC	1 cut(s)	914
PfeI	GAWTC	5 cut(s)	140, 194, 506, 532, 1498
PflMI	CCANNNNNTGG	1 cut(s)	1701
PfoI	TCCNGGA	1 cut(s)	358
PleI	GAGTC	9 cut(s)	136, 259, 419, 425, 451, 605, 1149, 1779, 1904
PpsI	GAGTC	9 cut(s)	136, 259, 419, 425, 451, 605, 1149, 1779, 1904
Ppu21I	YACGTR	1 cut(s)	709
PpuMI	RGGWCCY	2 cut(s)	866, 1475
PscI	ACATGT	2 cut(s)	661, 1857
PsiI	TTATAA	1 cut(s)	768
Psp1406I	AACGTT	1 cut(s)	2016
Psp5II	RGGWCCY	2 cut(s)	866, 1475
Psp6I	CCWGG	3 cut(s)	1331, 1522, 1813
PspGI	CCWGG	3 cut(s)	1331, 1522, 1813
PspN4I	GGNNCC	4 cut(s)	867, 970, 1360, 2023
PspPI	GGNCC	7 cut(s)	473, 866, 968, 1103, 1359, 1475, 2021
PspPPI	RGGWCCY	2 cut(s)	866, 1475
PstNI	CAGNNNCTG	4 cut(s)	446, 1019, 1523, 2228
PsuI	RGATCY	1 cut(s)	1391
PvuII	CAGCTG	1 cut(s)	956
RsaI	GTAC	5 cut(s)	291, 401, 1189, 1856, 2039
RsaNI	GTAC	5 cut(s)	290, 400, 1188, 1855, 2038
RseI	CAYNNNNRTG	2 cut(s)	837, 2072
SalI	GTCGAC	1 cut(s)	1444
SaqAI	TTAA	6 cut(s)	456, 809, 1280, 1503, 2028, 2070
Sau3AI	GATC	7 cut(s)	57, 1391, 1711, 1762, 1945, 2053, 2123
Sau96I	GGNCC	7 cut(s)	473, 866, 968, 1103, 1359, 1475, 2021
SchI	GAGTC	9 cut(s)	137, 260, 419, 426, 452, 606, 1150, 1779, 1905
ScrFI	CCNGG	5 cut(s)	360, 747, 1333, 1524, 1815
SduI	GDGCHC	3 cut(s)	745, 1638, 1865
SexAI	ACCWGGT	2 cut(s)	1522, 1813
SfaNI	GCATC	1 cut(s)	1232
SfcI	CTRYAG	3 cut(s)	39, 1047, 1245
Sfr274I	CTCGAG	1 cut(s)	1893
SfuI	TTCGAA	1 cut(s)	989
SinI	GGWCC	6 cut(s)	473, 866, 1103, 1359, 1475, 2021
SlaI	CTCGAG	1 cut(s)	1893
SmiMI	CAYNNNNRTG	2 cut(s)	837, 2072
SmlI	CTYRAG	3 cut(s)	1029, 1502, 1893
SmoI	CTYRAG	3 cut(s)	1029, 1502, 1893
SnaBI	TACGTA	1 cut(s)	709
SpeI	ACTAGT	1 cut(s)	2095
SphI	GCATGC	1 cut(s)	777
Sse9I	AATT	8 cut(s)	267, 364, 590, 936, 948, 1480, 1951, 1968
SseBI	AGGCCT	1 cut(s)	714
SsiI	CCGC	7 cut(s)	498, 738, 923, 1101, 1251, 1750, 2090
SspI	AATATT	1 cut(s)	1731
SspMI	CTAG	5 cut(s)	870, 1355, 1538, 1757, 2096
StuI	AGGCCT	1 cut(s)	714
StyD4I	CCNGG	5 cut(s)	358, 745, 1331, 1522, 1813
StyI	CCWWGG	1 cut(s)	1001
TaaI	ACNGT	7 cut(s)	1015, 1037, 1187, 1877, 1915, 2008, 2134
TaiI	ACGT	4 cut(s)	711, 1851, 2019, 2076
TaqII	GACCGA	1 cut(s)	1376
TasI	AATT	8 cut(s)	267, 364, 590, 936, 948, 1480, 1951, 1968
TatI	WGTACW	4 cut(s)	289, 1187, 1854, 2037
TauI	GCSGC	3 cut(s)	501, 1253, 1752
TfiI	GAWTC	5 cut(s)	140, 194, 506, 532, 1498
Tru1I	TTAA	6 cut(s)	456, 809, 1280, 1503, 2028, 2070
Tru9I	TTAA	6 cut(s)	456, 809, 1280, 1503, 2028, 2070
TscAI	CASTG	6 cut(s)	142, 253, 1020, 1882, 2137, 2170
TseFI	GTSAC	7 cut(s)	133, 162, 852, 1154, 1877, 1990, 2008
TseI	GCWGC	7 cut(s)	236, 953, 1019, 1220, 1241, 1517, 1541
Tsp45I	GTSAC	7 cut(s)	133, 162, 852, 1154, 1877, 1990, 2008
TspDTI	ATGAA	3 cut(s)	524, 743, 837
TspGWI	ACGGA	5 cut(s)	895, 908, 1429, 1755, 1791
TspRI	CASTG	6 cut(s)	142, 253, 1020, 1882, 2137, 2170
Van91I	CCANNNNNTGG	1 cut(s)	1701
Vha464I	CTTAAG	1 cut(s)	1502
VpaK11BI	GGWCC	6 cut(s)	473, 866, 1103, 1359, 1475, 2021
XagI	CCTNNNNNAGG	2 cut(s)	383, 2061
XapI	RAATTY	5 cut(s)	267, 364, 590, 936, 1951
XceI	RCATGY	3 cut(s)	665, 777, 1861
XcmI	CCANNNNNNNNNTGG	2 cut(s)	19, 1189
XhoI	CTCGAG	1 cut(s)	1893
XmiI	GTMKAC	1 cut(s)	1445
XspI	CTAG	5 cut(s)	870, 1355, 1538, 1757, 2096

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.