pycom14g11450

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0001127
Physical Location & Seq
Forward (+)
14530556 .. 14535600
5045 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g11450.4

Sequence Viewer

Length: 2286 bp
ATGAGCAACCCAAGTCCCCTAGTTGTTCTAGTCCTCTGGCTTCTCTGCTGTCTTAATAGCCAGGGCATGACAGAAAGAAGCAATGTACACATTGTTTATCTCGGAGAGACGCAGCATGACAACCCCGAGCTGGTCGTTGATTTGCATCATGAATTGCTTGCCTCTGTAGTCGGAAGCAAGGAATTGGCCTCAGAATTAATGGTATACAGTTACAAACATGGTTTCTCTGGGTTTGCAGCCAAGCTCACAGAGTCTCAAGTCCAACAACTTTCAGAGTTGCCTGGTGTTGTACGAATCATACCGAATAGCCTCCATAAGCTGGAAACAACTAGGAGTTGGAATTTTCTTGGCCTCTCTCCTCATTCCCCTTCCAATATTCTCCCCAAGAGCAAGATGGGGGATGGAGTTATCATAGGTGTTCTTGACACAGGTATTTGGCCAGAGTCTGAGTCATTTAATGAAAAAGGCCTTGGGCCAGTTCCATTCCATTGGAAGGGCGCGTGTGAATCTGGGGAAAATTTCAATACAACAATACATTGCAACAAAAAAATAATTGGAGCCCGTTGGTTCATCAAGGGACTTCTTGCAGAGTATGGAGAGCCATTGGACACATCCACTGACTTCTTGTCTCCTAGAGATGCAAATGGACATGGTACTGATATTTCAAGCATTGCAGCTGGTTCATTTGTTTACAATATTAGCTACAAGGGCCTTGGTGGTGGGACAGTTAGAGGGGGTGCCCCAAATGCCCGATTAGCCATGTACAAGGTTTGCTGGAATCTGTTTAATGGCTATTGCTCATCCGCTGACATACTTAAAGCTGTGGATGTAGCCATACATGAAGGGGTTCATGTGTTGTCATTATCATTAGGAGATTCAATACCCATATCCACTATTGTTGATGATGCAATTGCTATTGGTTCCTACCATGCTGTAACTAGAGACATTATCGTTGTGTGTTCGGCTGGAAACGATGGACCTTTTGCTCAAACAGTTACAAATACTGCACCATGGGTCATAACTGTTGCTGCAAGCACCATGGATCGAGCATTTCCTACTTCTATTACTTTAGGAAATAATATAACTTTGCAGGGTCAATCATTGTTTACAGGTCCGCATACCGGATTTACACGCTTGATATACCCAAAATCCACAAAGATTGACTCCACATCCACAAGTATATGTGATTCCCTCTCGTCCCTTAATAAAACTATGGTATCTGGAAATGTGGTTCTCTGCTTCACTAGTTACTCAGAAGAAGCTTTGAGAGAAGCAGGTTGTGTTGGCGTAATCATAGCAACGAACCCAAGTGATCTCATGCTTCCAAGTGATGACGATTTTCCGAATATTCAAGTTGACTATGAGGTTGGCACCCGCATATTATCTTATATCCGGTCTACTAGTGCTCCTCTTGTAAAGTTGAGCCGTACTAAAACAATAGTGGGCAAGCTAATATCAGCCAAAGTGGCCGCTTTTTCATCGAGGGGGCCAAACTCCGCCACACCAGCAATTCTCAAGCCAGATGTTGCCGCACCTGGTGTGAACATCTTAGCTGCATATTCTCCTCTTCGTTCCTATGTCGACGGCGGATATGCTGTGAAGTCAGGAACTTCATTGTCAACTCCTCATGTCTCAGGCATTGTAGCCCTTCTCAAAGTAATGCATCCTAGTTGGTCCCCAGCAGCCATTCGATCGGCACTTGTGACAACCGCGTCGAGGAATGGTCCATCTTGTAGTATTCTACCAATCTTTGCAGAGGGATCTCCTAGAAAGTTGGCTAATCCGTTTGATTACGGAGGCGGAATTGTGAACCCTAACAAAGCAGCATATCCTGGGTTAGTATACGATATGGGGAGAGAGGACTACATAAACTTCCTTTGTGCTGTTGGCTACAACACCTCAGCTCTCTCTCAAATTGTAGGACAAGTCACAGCATGTTCCAGTACAAAGCCTTCAGTTCTTGATGTGAACCTGCCTTCCATCACCATTCCAAACCTGAGAGACAATATAAACCTAACCAGAAGCGTCACAAATGTTGGTCCAGTGGACTCAGTATATAAAGTTGACATAGACCCTCCACTTGGCGTTAACGTAGCTGTAAGACCTGACACTTTGGTCTTCAATTCTTCCGTTAAAACAATCACGTTTACAGTTGCAGTTTCTACCACTCATCACGTGAACACCGGATATTACTTCGGCAGTCTCACTTGGACAGATGGGAAACATGTGGTCACAAGTGCCCTATCAGTTAGAACGCAAATTGCGGACTATACAGATGGATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

762

Amino Acids

81.06

Weight (kDa)

6.34

Isoelectric Point (pI)

34.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1711, 2114
Acc36I ACCTGC 2 cut(s) 1265, 1980
AccB1I GGYRCC 2 cut(s) 737, 1370
AccB7I CCANNNNNTGG 1 cut(s) 319
AccI GTMKAC 4 cut(s) 204, 1397, 1581, 1842
AccII CGCG 2 cut(s) 500, 1712
AclWI GGATC 2 cut(s) 1050, 1768
AcoI YGGCCR 2 cut(s) 437, 1467
AcsI RAATTY 2 cut(s) 340, 517
AcuI CTGAAG 1 cut(s) 1938
AcvI CACGTG 1 cut(s) 2176
AdeI CACNNNGTG 1 cut(s) 1538
AfaI GTAC 6 cut(s) 87, 291, 655, 764, 1429, 1945
AfiI CCNNNNNNNGG 6 cut(s) 130, 319, 493, 1121, 1716, 2081
AflIII ACRYGT 1 cut(s) 2224
AgsI TTSAA 5 cut(s) 523, 666, 879, 1352, 2122
AhdI GACNNNNNGTC 1 cut(s) 625
AhlI ACTAGT 2 cut(s) 1244, 1400
AjnI CCWGG 4 cut(s) 60, 280, 1534, 1831
AjuI GAANNNNNNNTTGG 2 cut(s) 453, 485
AloI GAACNNNNNNTCC 4 cut(s) 1215, 1247, 1961, 1993
Alw21I GWGCWC 1 cut(s) 1408
Alw26I GTCTC 7 cut(s) 101, 258, 633, 936, 1636, 1995, 2207
AlwI GGATC 2 cut(s) 1050, 1768
AlwNI CAGNNNCTG 1 cut(s) 446
Ama87I CYCGRG 1 cut(s) 125
AoxI GGCC 8 cut(s) 186, 349, 437, 466, 473, 709, 1467, 1487
ApeKI GCWGC 7 cut(s) 112, 236, 674, 1028, 1553, 1682, 1823
ApoI RAATTY 2 cut(s) 340, 517
AseI ATTAAT 1 cut(s) 197
Asp700I GAANNNNTTC 1 cut(s) 846
AspLEI GCGC 1 cut(s) 500
AspS9I GGNCC 8 cut(s) 473, 709, 977, 1112, 1487, 1674, 1724, 2039
AsuHPI GGTGA 1 cut(s) 1975
AvaI CYCGRG 1 cut(s) 125
AvaII GGWCC 5 cut(s) 977, 1112, 1674, 1724, 2039
BaeGI GKGCMC 2 cut(s) 742, 2242
BaeI ACNNNNGTAYC 2 cut(s) 1200, 1233
BalI TGGCCA 1 cut(s) 439
BanI GGYRCC 2 cut(s) 737, 1370
BanII GRGCYC 1 cut(s) 562
BarI GAAGNNNNNNTAC 2 cut(s) 1936, 1968
BbrPI CACGTG 1 cut(s) 2176
BbsI GAAGAC 1 cut(s) 2110
Bbv12I GWGCWC 1 cut(s) 1408
BbvCI CCTCAGC 1 cut(s) 1900
BbvI GCAGC 7 cut(s) 124, 248, 686, 1015, 1540, 1694, 1835
BccI CCATC 7 cut(s) 388, 395, 968, 1735, 1988, 2210, 2270
BceAI ACGGC 2 cut(s) 1410, 1600
BciT130I CCWGG 4 cut(s) 62, 282, 1536, 1833
BciVI GTATCC 1 cut(s) 2273
BcoDI GTCTC 7 cut(s) 101, 258, 633, 936, 1636, 1995, 2207
BcuI ACTAGT 2 cut(s) 1244, 1400
BfaI CTAG 9 cut(s) 20, 29, 330, 633, 939, 1245, 1401, 1668, 1767
BfmI CTRYAG 1 cut(s) 165
BfuAI ACCTGC 2 cut(s) 1265, 1980
BfuI GTATCC 1 cut(s) 2273
BglI GCCNNNNNGGC 1 cut(s) 1466
BisI GCNGC 9 cut(s) 113, 237, 675, 1029, 1470, 1530, 1554, 1683, 1824
BlsI GCNGC 9 cut(s) 114, 238, 676, 1030, 1471, 1531, 1555, 1684, 1825
Bme1390I CCNGG 4 cut(s) 62, 282, 1536, 1833
Bme18I GGWCC 5 cut(s) 977, 1112, 1674, 1724, 2039
BmeRI GACNNNNNGTC 1 cut(s) 625
BmeT110I CYCGRG 1 cut(s) 125
BmgT120I GGNCC 8 cut(s) 473, 709, 977, 1112, 1487, 1674, 1724, 2039
BmiI GGNNCC 6 cut(s) 559, 739, 922, 1372, 1488, 1676
BmrFI CCNGG 4 cut(s) 62, 282, 1536, 1833
BmsI GCATC 4 cut(s) 154, 628, 895, 1672
BpiI GAAGAC 1 cut(s) 2110
Bpu10I CCTNAGC 1 cut(s) 1900
BpuEI CTTGAG 2 cut(s) 240, 1499
BsaAI YACGTR 1 cut(s) 2176
BsaBI GATNNNNATC 1 cut(s) 144
BsaJI CCNNGG 6 cut(s) 61, 469, 712, 1010, 1038, 1832
BsaWI WCCGGW 3 cut(s) 1121, 1392, 2183
BsaXI ACNNNNNCTCC 2 cut(s) 1390, 1420
Bsc4I CCNNNNNNNGG 6 cut(s) 130, 319, 493, 1121, 1716, 2081
Bse1I ACTGG 3 cut(s) 476, 1941, 2042
Bse3DI GCAATG 3 cut(s) 88, 535, 669
Bse8I GATNNNNATC 1 cut(s) 144
BseBI CCWGG 4 cut(s) 62, 282, 1536, 1833
BseDI CCNNGG 6 cut(s) 61, 469, 712, 1010, 1038, 1832
BseGI GGATG 6 cut(s) 406, 611, 800, 832, 1169, 1663
BseJI GATNNNNATC 1 cut(s) 144
BseLI CCNNNNNNNGG 6 cut(s) 130, 319, 493, 1121, 1716, 2081
BseMI GCAATG 3 cut(s) 88, 535, 669
BseMII CTCAG 7 cut(s) 204, 438, 1266, 1647, 1914, 1988, 2064
BseNI ACTGG 3 cut(s) 476, 1941, 2042
BseRI GAGGAG 4 cut(s) 348, 1398, 1554, 1614
BseSI GKGCMC 2 cut(s) 742, 2242
BseXI GCAGC 7 cut(s) 124, 248, 686, 1015, 1540, 1694, 1835
BseYI CCCAGC 1 cut(s) 1678
BsgI GTGCAG 1 cut(s) 990
Bsh1236I CGCG 2 cut(s) 500, 1712
Bsh1285I CGRYCG 1 cut(s) 1694
BshFI GGCC 8 cut(s) 188, 351, 439, 468, 475, 711, 1469, 1489
BshNI GGYRCC 2 cut(s) 737, 1370
BsiEI CGRYCG 1 cut(s) 1694
BsiHKAI GWGCWC 1 cut(s) 1408
BsiHKCI CYCGRG 1 cut(s) 125
BsiSI CCGG 3 cut(s) 1122, 1393, 2184
BslFI GGGAC 4 cut(s) 591, 736, 1183, 1660
BslI CCNNNNNNNGG 6 cut(s) 130, 319, 493, 1121, 1716, 2081
BsmAI GTCTC 7 cut(s) 101, 258, 633, 936, 1636, 1995, 2207
BsmBI CGTCTC 1 cut(s) 101
BsmFI GGGAC 4 cut(s) 591, 736, 1183, 1660
BsnI GGCC 8 cut(s) 188, 351, 439, 468, 475, 711, 1469, 1489
BsoBI CYCGRG 1 cut(s) 125
Bsp1286I GDGCHC 4 cut(s) 562, 742, 1408, 2242
Bsp1407I TGTACA 2 cut(s) 85, 762
Bsp143I GATC 4 cut(s) 1042, 1312, 1691, 1760
Bsp19I CCATGG 2 cut(s) 1010, 1038
BspANI GGCC 8 cut(s) 188, 351, 439, 468, 475, 711, 1469, 1489
BspCNI CTCAG 7 cut(s) 203, 439, 1265, 1646, 1913, 1989, 2063
BspFNI CGCG 2 cut(s) 500, 1712
BspHI TCATGA 1 cut(s) 148
BspLI GGNNCC 6 cut(s) 559, 739, 922, 1372, 1488, 1676
BspMI ACCTGC 2 cut(s) 1265, 1980
BspPI GGATC 2 cut(s) 1050, 1768
BspT107I GGYRCC 2 cut(s) 737, 1370
BsrDI GCAATG 3 cut(s) 88, 535, 669
BsrGI TGTACA 2 cut(s) 85, 762
BsrI ACTGG 3 cut(s) 476, 1941, 2042
BssECI CCNNGG 6 cut(s) 61, 469, 712, 1010, 1038, 1832
BssMI GATC 4 cut(s) 1042, 1312, 1691, 1760
BssNAI GTATAC 2 cut(s) 205, 1843
BssT1I CCWWGG 4 cut(s) 469, 712, 1010, 1038
Bst1107I GTATAC 2 cut(s) 205, 1843
Bst2UI CCWGG 4 cut(s) 62, 282, 1536, 1833
Bst4CI ACNGT 5 cut(s) 209, 727, 994, 1024, 2152
Bst6I CTCTTC 1 cut(s) 1572
BstAUI TGTACA 2 cut(s) 85, 762
BstBAI YACGTR 1 cut(s) 2176
BstC8I GCNNGC 3 cut(s) 159, 1033, 1448
BstDEI CTNAG 8 cut(s) 190, 447, 1252, 1549, 1633, 1900, 1997, 2050
BstDSI CCRYGG 2 cut(s) 1010, 1038
BstF5I GGATG 6 cut(s) 406, 611, 800, 832, 1169, 1663
BstFNI CGCG 2 cut(s) 500, 1712
BstHHI GCGC 1 cut(s) 500
BstKTI GATC 4 cut(s) 1045, 1315, 1694, 1763
BstMAI GTCTC 7 cut(s) 101, 258, 633, 936, 1636, 1995, 2207
BstMBI GATC 4 cut(s) 1042, 1312, 1691, 1760
BstMCI CGRYCG 1 cut(s) 1694
BstMWI GCNNNNNNNGC 5 cut(s) 708, 746, 755, 1466, 1505
BstNI CCWGG 4 cut(s) 62, 282, 1536, 1833
BstNSI RCATGY 2 cut(s) 1938, 2228
BstSCI CCNGG 4 cut(s) 60, 280, 1534, 1831
BstSFI CTRYAG 1 cut(s) 165
BstSLI GKGCMC 2 cut(s) 742, 2242
BstUI CGCG 2 cut(s) 500, 1712
BstV1I GCAGC 7 cut(s) 124, 248, 686, 1015, 1540, 1694, 1835
BstV2I GAAGAC 1 cut(s) 2110
BstX2I RGATCY 1 cut(s) 1760
BstXI CCANNNNNNTGG 1 cut(s) 489
BstYI RGATCY 1 cut(s) 1760
BstZ17I GTATAC 2 cut(s) 205, 1843
BsuI GTATCC 1 cut(s) 2273
BsuRI GGCC 8 cut(s) 188, 351, 439, 468, 475, 711, 1469, 1489
BtgI CCRYGG 2 cut(s) 1010, 1038
BtsCI GGATG 6 cut(s) 406, 611, 800, 832, 1169, 1663
BtsIMutI CAGTG 2 cut(s) 615, 2049
BveI ACCTGC 2 cut(s) 1265, 1980
Cac8I GCNNGC 3 cut(s) 159, 1033, 1448
CaiI CAGNNNCTG 1 cut(s) 446
CciI TCATGA 1 cut(s) 148
CfoI GCGC 1 cut(s) 500
Cfr13I GGNCC 8 cut(s) 473, 709, 977, 1112, 1487, 1674, 1724, 2039
CseI GACGC 3 cut(s) 118, 1701, 2014
CsiI ACCWGGT 1 cut(s) 1534
Csp6I GTAC 6 cut(s) 86, 290, 654, 763, 1428, 1944
CspCI CAANNNNNGTGG 4 cut(s) 880, 915, 1141, 1176
CviQI GTAC 6 cut(s) 86, 290, 654, 763, 1428, 1944
DdeI CTNAG 8 cut(s) 190, 447, 1252, 1549, 1633, 1900, 1997, 2050
DpnI GATC 4 cut(s) 1044, 1314, 1693, 1762
DpnII GATC 4 cut(s) 1042, 1312, 1691, 1760
DraIII CACNNNGTG 1 cut(s) 1538
DrdI GACNNNNNNGTC 2 cut(s) 1711, 2114
DriI GACNNNNNGTC 1 cut(s) 625
DseDI GACNNNNNNGTC 2 cut(s) 1711, 2114
EaeI YGGCCR 2 cut(s) 437, 1467
Eam1104I CTCTTC 1 cut(s) 1572
Eam1105I GACNNNNNGTC 1 cut(s) 625
EarI CTCTTC 1 cut(s) 1572
EciI GGCGGA 3 cut(s) 1486, 1602, 1815
Eco130I CCWWGG 4 cut(s) 469, 712, 1010, 1038
Eco147I AGGCCT 1 cut(s) 468
Eco24I GRGCYC 1 cut(s) 562
Eco47I GGWCC 5 cut(s) 977, 1112, 1674, 1724, 2039
Eco57I CTGAAG 1 cut(s) 1938
Eco72I CACGTG 1 cut(s) 2176
Eco88I CYCGRG 1 cut(s) 125
EcoO109I RGGNCCY 1 cut(s) 709
EcoRII CCWGG 4 cut(s) 60, 280, 1534, 1831
EcoT14I CCWWGG 4 cut(s) 469, 712, 1010, 1038
EcoT22I ATGCAT 1 cut(s) 1665
EcoT38I GRGCYC 1 cut(s) 562
ErhI CCWWGG 4 cut(s) 469, 712, 1010, 1038
Esp3I CGTCTC 1 cut(s) 101
FaqI GGGAC 4 cut(s) 591, 736, 1183, 1660
FauI CCCGC 1 cut(s) 1382
FblI GTMKAC 4 cut(s) 204, 1397, 1581, 1842
Fnu4HI GCNGC 9 cut(s) 113, 237, 675, 1029, 1470, 1530, 1554, 1683, 1824
FokI GGATG 6 cut(s) 413, 598, 787, 839, 1156, 1650
FriOI GRGCYC 1 cut(s) 562
Fsp4HI GCNGC 9 cut(s) 113, 237, 675, 1029, 1470, 1530, 1554, 1683, 1824
FspBI CTAG 9 cut(s) 20, 29, 330, 633, 939, 1245, 1401, 1668, 1767
GlaI GCGC 1 cut(s) 499
GluI GCNGC 9 cut(s) 113, 237, 675, 1029, 1470, 1530, 1554, 1683, 1824
GsaI CCCAGC 1 cut(s) 1682
HaeIII GGCC 8 cut(s) 188, 351, 439, 468, 475, 711, 1469, 1489
HapII CCGG 3 cut(s) 1122, 1393, 2184
HgaI GACGC 3 cut(s) 118, 1701, 2014
HhaI GCGC 1 cut(s) 500
Hin6I GCGC 1 cut(s) 498
HinP1I GCGC 1 cut(s) 498
HincII GTYRAC 5 cut(s) 1357, 1582, 1620, 2065, 2089
HindII GTYRAC 5 cut(s) 1357, 1582, 1620, 2065, 2089
HindIII AAGCTT 1 cut(s) 1260
HpaI GTTAAC 1 cut(s) 2089
HpaII CCGG 3 cut(s) 1122, 1393, 2184
HphI GGTGA 1 cut(s) 1975
Hpy188I TCNGA 7 cut(s) 104, 173, 193, 274, 448, 1255, 1344
Hpy188III TCNNGA 5 cut(s) 149, 422, 1221, 1605, 1961
Hpy99I CGWCG 2 cut(s) 1586, 1717
HpyAV CCTTC 6 cut(s) 378, 487, 836, 1658, 1962, 1986
HpyCH4III ACNGT 5 cut(s) 209, 727, 994, 1024, 2152
HpyCH4IV ACGT 3 cut(s) 2091, 2144, 2175
HpyF10VI GCNNNNNNNGC 5 cut(s) 708, 746, 755, 1466, 1505
HpyF3I CTNAG 8 cut(s) 190, 447, 1252, 1549, 1633, 1900, 1997, 2050
HpySE526I ACGT 3 cut(s) 2091, 2144, 2175
HspAI GCGC 1 cut(s) 498
KspAI GTTAAC 1 cut(s) 2089
Kzo9I GATC 4 cut(s) 1042, 1312, 1691, 1760
LmnI GCTCC 2 cut(s) 557, 1411
Lsp1109I GCAGC 7 cut(s) 124, 248, 686, 1015, 1540, 1694, 1835
LweI GCATC 4 cut(s) 154, 628, 895, 1672
MabI ACCWGGT 1 cut(s) 1534
MaeI CTAG 9 cut(s) 20, 29, 330, 633, 939, 1245, 1401, 1668, 1767
MaeII ACGT 3 cut(s) 2091, 2144, 2175
MaeIII GTNAC 8 cut(s) 209, 934, 994, 1247, 1702, 1927, 2026, 2230
MalI GATC 4 cut(s) 1044, 1314, 1693, 1762
MboI GATC 4 cut(s) 1042, 1312, 1691, 1760
MboII GAAGA 4 cut(s) 1268, 1559, 2110, 2118
MfeI CAATTG 1 cut(s) 909
MflI RGATCY 1 cut(s) 1760
MhlI GDGCHC 4 cut(s) 562, 742, 1408, 2242
MlsI TGGCCA 1 cut(s) 439
MluNI TGGCCA 1 cut(s) 439
MlyI GAGTC 5 cut(s) 260, 452, 458, 1157, 2042
MmeI TCCRAC 3 cut(s) 151, 286, 317
Mox20I TGGCCA 1 cut(s) 439
Mph1103I ATGCAT 1 cut(s) 1665
MroXI GAANNNNTTC 1 cut(s) 846
MscI TGGCCA 1 cut(s) 439
MseI TTAA 8 cut(s) 54, 197, 456, 786, 816, 1203, 2088, 2133
Msp20I TGGCCA 1 cut(s) 439
MspA1I CMGCKG 2 cut(s) 677, 806
MspI CCGG 3 cut(s) 1122, 1393, 2184
MspR9I CCNGG 4 cut(s) 62, 282, 1536, 1833
MunI CAATTG 1 cut(s) 909
MvaI CCWGG 4 cut(s) 62, 282, 1536, 1833
MvnI CGCG 2 cut(s) 500, 1712
MwoI GCNNNNNNNGC 5 cut(s) 708, 746, 755, 1466, 1505
NcoI CCATGG 2 cut(s) 1010, 1038
NdeII GATC 4 cut(s) 1042, 1312, 1691, 1760
NlaIV GGNNCC 6 cut(s) 559, 739, 922, 1372, 1488, 1676
NmuCI GTSAC 4 cut(s) 1702, 1927, 2026, 2230
NsiI ATGCAT 1 cut(s) 1665
NspI RCATGY 2 cut(s) 1938, 2228
PagI TCATGA 1 cut(s) 148
PceI AGGCCT 1 cut(s) 468
PciI ACATGT 1 cut(s) 2224
PdmI GAANNNNTTC 1 cut(s) 846
PfeI GAWTC 5 cut(s) 294, 506, 778, 875, 1187
PflMI CCANNNNNTGG 1 cut(s) 319
PkrI GCNGC 9 cut(s) 114, 238, 676, 1030, 1471, 1531, 1555, 1684, 1825
Ple19I CGATCG 1 cut(s) 1694
PleI GAGTC 5 cut(s) 259, 451, 457, 1157, 2042
PmaCI CACGTG 1 cut(s) 2176
PmlI CACGTG 1 cut(s) 2176
PpsI GAGTC 5 cut(s) 259, 451, 457, 1157, 2042
Ppu21I YACGTR 1 cut(s) 2176
PscI ACATGT 1 cut(s) 2224
PshBI ATTAAT 1 cut(s) 197
Psp6I CCWGG 4 cut(s) 60, 280, 1534, 1831
PspCI CACGTG 1 cut(s) 2176
PspFI CCCAGC 1 cut(s) 1678
PspGI CCWGG 4 cut(s) 60, 280, 1534, 1831
PspN4I GGNNCC 6 cut(s) 559, 739, 922, 1372, 1488, 1676
PspPI GGNCC 8 cut(s) 473, 709, 977, 1112, 1487, 1674, 1724, 2039
PstNI CAGNNNCTG 1 cut(s) 446
PsuI RGATCY 1 cut(s) 1760
PvuI CGATCG 1 cut(s) 1694
PvuII CAGCTG 1 cut(s) 677
RsaI GTAC 6 cut(s) 87, 291, 655, 764, 1429, 1945
RsaNI GTAC 6 cut(s) 86, 290, 654, 763, 1428, 1944
SalI GTCGAC 1 cut(s) 1580
SaqAI TTAA 8 cut(s) 54, 197, 456, 786, 816, 1203, 2088, 2133
SatI GCNGC 9 cut(s) 113, 237, 675, 1029, 1470, 1530, 1554, 1683, 1824
Sau3AI GATC 4 cut(s) 1042, 1312, 1691, 1760
Sau96I GGNCC 8 cut(s) 473, 709, 977, 1112, 1487, 1674, 1724, 2039
SchI GAGTC 5 cut(s) 260, 452, 458, 1157, 2042
ScrFI CCNGG 4 cut(s) 62, 282, 1536, 1833
SduI GDGCHC 4 cut(s) 562, 742, 1408, 2242
SexAI ACCWGGT 1 cut(s) 1534
SfaNI GCATC 4 cut(s) 154, 628, 895, 1672
SfcI CTRYAG 1 cut(s) 165
SinI GGWCC 5 cut(s) 977, 1112, 1674, 1724, 2039
SmlI CTYRAG 2 cut(s) 255, 1514
SmoI CTYRAG 2 cut(s) 255, 1514
SpeI ACTAGT 2 cut(s) 1244, 1400
SseBI AGGCCT 1 cut(s) 468
SspI AATATT 3 cut(s) 376, 697, 1348
SspMI CTAG 9 cut(s) 20, 29, 330, 633, 939, 1245, 1401, 1668, 1767
StuI AGGCCT 1 cut(s) 468
StyD4I CCNGG 4 cut(s) 60, 280, 1534, 1831
StyI CCWWGG 4 cut(s) 469, 712, 1010, 1038
TaaI ACNGT 5 cut(s) 209, 727, 994, 1024, 2152
TaiI ACGT 3 cut(s) 2094, 2147, 2178
TaqI TCGA 5 cut(s) 1045, 1481, 1581, 1690, 1715
TatI WGTACW 3 cut(s) 85, 762, 1943
TauI GCSGC 2 cut(s) 1472, 1532
TfiI GAWTC 5 cut(s) 294, 506, 778, 875, 1187
Tru1I TTAA 8 cut(s) 54, 197, 456, 786, 816, 1203, 2088, 2133
Tru9I TTAA 8 cut(s) 54, 197, 456, 786, 816, 1203, 2088, 2133
TscAI CASTG 2 cut(s) 622, 2049
TseFI GTSAC 4 cut(s) 1702, 1927, 2026, 2230
TseI GCWGC 7 cut(s) 112, 236, 674, 1028, 1553, 1682, 1823
Tsp45I GTSAC 4 cut(s) 1702, 1927, 2026, 2230
TspDTI ATGAA 8 cut(s) 165, 474, 559, 672, 839, 855, 1467, 1602
TspGWI ACGGA 3 cut(s) 1773, 1809, 2119
TspRI CASTG 2 cut(s) 622, 2049
Van91I CCANNNNNTGG 1 cut(s) 319
VpaK11BI GGWCC 5 cut(s) 977, 1112, 1674, 1724, 2039
VspI ATTAAT 1 cut(s) 197
XapI RAATTY 2 cut(s) 340, 517
XceI RCATGY 2 cut(s) 1938, 2228
XcmI CCANNNNNNNNNTGG 1 cut(s) 391
XmiI GTMKAC 4 cut(s) 204, 1397, 1581, 1842
XmnI GAANNNNTTC 1 cut(s) 846
XspI CTAG 9 cut(s) 20, 29, 330, 633, 939, 1245, 1401, 1668, 1767
Zsp2I ATGCAT 1 cut(s) 1665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.