subtilisin-like protease
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AfaI | GTAC | 2 cut(s) | 18, 184 |
| AgsI | TTSAA | 1 cut(s) | 133 |
| AluBI | AGCT | 1 cut(s) | 207 |
| AluI | AGCT | 1 cut(s) | 207 |
| AspS9I | GGNCC | 1 cut(s) | 104 |
| AsuHPI | GGTGA | 2 cut(s) | 169, 200 |
| AvaII | GGWCC | 1 cut(s) | 104 |
| BanII | GRGCYC | 1 cut(s) | 44 |
| BccI | CCATC | 2 cut(s) | 122, 148 |
| BfaI | CTAG | 2 cut(s) | 99, 208 |
| BglII | AGATCT | 1 cut(s) | 23 |
| Bme18I | GGWCC | 1 cut(s) | 104 |
| BmgT120I | GGNCC | 1 cut(s) | 104 |
| BmsI | GCATC | 1 cut(s) | 152 |
| BsaJI | CCNNGG | 1 cut(s) | 177 |
| BseDI | CCNNGG | 1 cut(s) | 177 |
| BseGI | GGATG | 1 cut(s) | 143 |
| BseMII | CTCAG | 1 cut(s) | 29 |
| Bsp1286I | GDGCHC | 1 cut(s) | 44 |
| Bsp143I | GATC | 1 cut(s) | 23 |
| Bsp19I | CCATGG | 1 cut(s) | 177 |
| BspCNI | CTCAG | 1 cut(s) | 30 |
| BssECI | CCNNGG | 1 cut(s) | 177 |
| BssMI | GATC | 1 cut(s) | 23 |
| BssT1I | CCWWGG | 1 cut(s) | 177 |
| Bst6I | CTCTTC | 1 cut(s) | 128 |
| BstDEI | CTNAG | 2 cut(s) | 38, 48 |
| BstDSI | CCRYGG | 1 cut(s) | 177 |
| BstF5I | GGATG | 1 cut(s) | 143 |
| BstKTI | GATC | 1 cut(s) | 26 |
| BstMBI | GATC | 1 cut(s) | 23 |
| BstX2I | RGATCY | 1 cut(s) | 23 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 154 |
| BstYI | RGATCY | 1 cut(s) | 23 |
| BtgI | CCRYGG | 1 cut(s) | 177 |
| BtsCI | GGATG | 1 cut(s) | 143 |
| BtsIMutI | CAGTG | 1 cut(s) | 53 |
| Cfr13I | GGNCC | 1 cut(s) | 104 |
| Csp6I | GTAC | 2 cut(s) | 17, 183 |
| CviAII | CATG | 1 cut(s) | 178 |
| CviJI | RGCY | 4 cut(s) | 42, 67, 171, 207 |
| CviKI_1 | RGCY | 4 cut(s) | 42, 67, 171, 207 |
| CviQI | GTAC | 2 cut(s) | 17, 183 |
| DdeI | CTNAG | 2 cut(s) | 38, 48 |
| DpnI | GATC | 1 cut(s) | 25 |
| DpnII | GATC | 1 cut(s) | 23 |
| Eam1104I | CTCTTC | 1 cut(s) | 128 |
| EarI | CTCTTC | 1 cut(s) | 128 |
| Eco130I | CCWWGG | 1 cut(s) | 177 |
| Eco24I | GRGCYC | 1 cut(s) | 44 |
| Eco47I | GGWCC | 1 cut(s) | 104 |
| EcoO109I | RGGNCCY | 1 cut(s) | 104 |
| EcoT14I | CCWWGG | 1 cut(s) | 177 |
| EcoT38I | GRGCYC | 1 cut(s) | 44 |
| ErhI | CCWWGG | 1 cut(s) | 177 |
| FaeI | CATG | 1 cut(s) | 181 |
| FaiI | YATR | 1 cut(s) | 179 |
| FatI | CATG | 1 cut(s) | 177 |
| FokI | GGATG | 1 cut(s) | 130 |
| FriOI | GRGCYC | 1 cut(s) | 44 |
| FspBI | CTAG | 2 cut(s) | 99, 208 |
| Hin1II | CATG | 1 cut(s) | 181 |
| HinfI | GANTC | 1 cut(s) | 76 |
| HphI | GGTGA | 2 cut(s) | 169, 200 |
| Hpy166II | GTNNAC | 1 cut(s) | 19 |
| Hpy188III | TCNNGA | 1 cut(s) | 200 |
| Hpy8I | GTNNAC | 1 cut(s) | 19 |
| HpyAV | CCTTC | 1 cut(s) | 54 |
| HpyCH4V | TGCA | 1 cut(s) | 125 |
| HpyF3I | CTNAG | 2 cut(s) | 38, 48 |
| Hsp92II | CATG | 1 cut(s) | 181 |
| Kzo9I | GATC | 1 cut(s) | 23 |
| LpnPI | CCDG | 2 cut(s) | 81, 135 |
| LweI | GCATC | 1 cut(s) | 152 |
| MaeI | CTAG | 2 cut(s) | 99, 208 |
| MalI | GATC | 1 cut(s) | 25 |
| MboI | GATC | 1 cut(s) | 23 |
| MboII | GAAGA | 1 cut(s) | 145 |
| MflI | RGATCY | 1 cut(s) | 23 |
| MhlI | GDGCHC | 1 cut(s) | 44 |
| MluCI | AATT | 2 cut(s) | 109, 126 |
| MlyI | GAGTC | 1 cut(s) | 85 |
| MnlI | CCTC | 1 cut(s) | 95 |
| NcoI | CCATGG | 1 cut(s) | 177 |
| NdeII | GATC | 1 cut(s) | 23 |
| NlaIII | CATG | 1 cut(s) | 181 |
| PleI | GAGTC | 1 cut(s) | 84 |
| PpsI | GAGTC | 1 cut(s) | 84 |
| PpuMI | RGGWCCY | 1 cut(s) | 104 |
| Psp5II | RGGWCCY | 1 cut(s) | 104 |
| PspPI | GGNCC | 1 cut(s) | 104 |
| PspPPI | RGGWCCY | 1 cut(s) | 104 |
| PsuI | RGATCY | 1 cut(s) | 23 |
| RsaI | GTAC | 2 cut(s) | 18, 184 |
| RsaNI | GTAC | 2 cut(s) | 17, 183 |
| Sau3AI | GATC | 1 cut(s) | 23 |
| Sau96I | GGNCC | 1 cut(s) | 104 |
| SchI | GAGTC | 1 cut(s) | 85 |
| SduI | GDGCHC | 1 cut(s) | 44 |
| SetI | ASST | 2 cut(s) | 109, 209 |
| SfaNI | GCATC | 1 cut(s) | 152 |
| SgeI | CNNG | 7 cut(s) | 80, 87, 92, 111, 134, 190, 198 |
| SinI | GGWCC | 1 cut(s) | 104 |
| Sse9I | AATT | 2 cut(s) | 109, 126 |
| SspMI | CTAG | 2 cut(s) | 99, 208 |
| StyI | CCWWGG | 1 cut(s) | 177 |
| TaqI | TCGA | 1 cut(s) | 74 |
| TasI | AATT | 2 cut(s) | 109, 126 |
| TscAI | CASTG | 1 cut(s) | 60 |
| TspRI | CASTG | 1 cut(s) | 60 |
| VpaK11BI | GGWCC | 1 cut(s) | 104 |
| XspI | CTAG | 2 cut(s) | 99, 208 |