Rmu_sc0003144.1_g000021

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003144.1
OG0001127
Physical Location & Seq
Forward (+)
114029 .. 117295
3267 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003144.1_g000021.1.cds

Sequence Viewer

Length: 2334 bp
atgacctgcccaataagttcagtagtacttggtttcctattatgccttctctgtgttctgaatgaccagggaatatcgataacagtggccgcggctaagactaatagccgtgttcacattgtgtatctgggagagaggcagcatgacaatcccaagttgatcacggattctcatcatgatttgcttgccaccatagttggaagtaagagcttggcctcggaattgatggtatacagttacagacatggtttctctggatttgcagccaagcttacggaggcgcaagcccaaaaatgtgcagagttgcctgatgttgttcgagtcataccaaatactctatacaagctgcaaactactaggagttgggattttcttggcctctctcctcagtcaccttcaagtaatattcttcccagtagcgacatgggtgatggagttatcataggtgtccttgactcaggtatatggccagagtctaaatctttcaatgaagaaggcctagggccagttccatcccactggaagggtatctgtgaatccggtgaaaaatttaatgccacattgcattgcaatagaaaaatcattggagcacgttggttcaacgatggaatactcgctgagtatggacagccgctgaacacatctgaaagaactgaattcatgtccccaagagatgcacatggacatggcactcacacagctagcactgcagctggttcctttgtgactaatgttagttacaagggccttggtcatgggacagttagagggggtgccccaaatgctcgattggccatttacaaggtttgctggaatgtgcttgtcagtctatgctcaggagctgatatactcaaagcttttgatgaagccatacatgatggagttgatgtattgtcactctcgattgggggttcagttccactctattcggatgtggatgaacgtgatgtcattgctactggctcctttcatgctgtggccaggggaattactgttgtttgtgcagcatcaaatgatggaccttcggcgcagacagttcagaacacatcaccttggatcataactgttgcagcaagcaccacggatcgagcatttcctacttccataactttaggaaataacaaaactttcctgggtcaagatatgtttacaggacttgagattggttttacaagcttgatatatccggagtctggagggcttgaacctactgctacaggtgtctgtgaatccctctcacttaacaaaaccatggtatctggaaaggtggttctatgcttcacttcactcggtcgtaggagagctgtaacaagtgcttcagctgctgtgaaagaagctggcggtgttggcctaatcgtagcaaagaatccaactgatggcttgtatccatgtagtgatgactttccttgtattgaagttgactatgagataggcacccgaataatgttttatatccgatctaccagatatcctcttgtgaagttgaaccgttcgaaaacaattgcgggcaagccaatattagccaaggttgccgatttttcatctcggggaccaaactccgcagcaccagcaattctcaagccagatatagctgcgcctggtgtgaacatattagctgcaacttctccactgcattcatttgtggatgctgggtatgtcatgctctcaggaacatcgatggcaactcctcatgtctcaggcattgtggctctcctcaaagcactgcatcctaactggtctccagcagccattcgatcagcactagtcacaagtgcatggaggaatggtccatctggtttaccaatttttgccgagggatctcctcaaaagttgaccaatcctttcgactttggaggtggcattgtgcacccgaatgcagcagcaaacccgggtcttgtatatgacatgggtgcagcagactacatacattatctatgtgcgatgggctacaaaaactctgccatatcaaggctcacaggccaagccacaacatgtcccataatgagaccttctattttggacattaacctaccttccataaccataccaagcctcagacattccattactgtaacaagaactgtcacaaatgtaggagactctaaatccgtttatgaagctacaattgatcctccattgggaacagtagtatctgtaaaacccaatcctttggtattcaactccacggttcagaaactcactttcgagattacaatttccacaacccaccagatgaacacagggtactactttggaagccttacatggactgatagagtgcatgctgtgagaattcccttgtctgtgaggacagtttttacaacattttgctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

777

Amino Acids

82.64

Weight (kDa)

7.57

Isoelectric Point (pI)

31.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 14
AccB1I GGYRCC 2 cut(s) 773, 1433
AccB7I CCANNNNNTGG 1 cut(s) 1376
AccI GTMKAC 1 cut(s) 231
AccII CGCG 1 cut(s) 92
AccIII TCCGGA 1 cut(s) 1186
AciI CCGC 6 cut(s) 90, 92, 632, 1341, 1505, 1560
AclWI GGATC 4 cut(s) 1064, 1092, 1825, 2123
AcoI YGGCCR 4 cut(s) 87, 467, 792, 978
AcsI RAATTY 3 cut(s) 548, 656, 2292
AcuI CTGAAG 1 cut(s) 1302
AdeI CACNNNGTG 1 cut(s) 121
AfaI GTAC 2 cut(s) 27, 2246
AfiI CCNNNNNNNGG 5 cut(s) 523, 1193, 1376, 1968, 2138
AflIII ACRYGT 1 cut(s) 1991
AgsI TTSAA 7 cut(s) 399, 487, 601, 1205, 1415, 1486, 2179
AhlI ACTAGT 1 cut(s) 1762
AjnI CCWGG 4 cut(s) 66, 980, 1131, 1597
AloI GAACNNNNNNTCC 2 cut(s) 1254, 1286
Alw21I GWGCWC 2 cut(s) 592, 1869
Alw26I GTCTC 4 cut(s) 1699, 1743, 1999, 2091
Alw44I GTGCAC 1 cut(s) 1865
AlwI GGATC 4 cut(s) 1064, 1092, 1825, 2123
AlwNI CAGNNNCTG 3 cut(s) 634, 842, 1325
Ama87I CYCGRG 2 cut(s) 1545, 1888
Aor13HI TCCGGA 1 cut(s) 1186
ApaLI GTGCAC 1 cut(s) 1865
ApoI RAATTY 3 cut(s) 548, 656, 2292
AspA2I CCTAGG 1 cut(s) 499
AspLEI GCGC 3 cut(s) 283, 1030, 1597
AspS9I GGNCC 5 cut(s) 503, 745, 1019, 1550, 1787
AsuC2I CCSGG 2 cut(s) 1889, 1890
AsuHPI GGTGA 4 cut(s) 384, 440, 554, 1041
AsuII TTCGAA 1 cut(s) 1493
AsuNHI GCTAGC 1 cut(s) 701
AvaI CYCGRG 2 cut(s) 1545, 1888
AvaII GGWCC 3 cut(s) 1019, 1550, 1787
AvrII CCTAGG 1 cut(s) 499
BaeGI GKGCMC 2 cut(s) 778, 1869
BaeI ACNNNNGTAYC 4 cut(s) 1239, 1272, 2133, 2166
BalI TGGCCA 3 cut(s) 469, 794, 980
BanI GGYRCC 2 cut(s) 773, 1433
Bbv12I GWGCWC 2 cut(s) 592, 1869
BceAI ACGGC 1 cut(s) 93
BciT130I CCWGG 4 cut(s) 68, 982, 1133, 1599
BciVI GTATCC 1 cut(s) 1395
BclI TGATCA 1 cut(s) 159
BcnI CCSGG 2 cut(s) 1889, 1890
BcoDI GTCTC 4 cut(s) 1699, 1743, 1999, 2091
BcuI ACTAGT 1 cut(s) 1762
BfaI CTAG 4 cut(s) 357, 500, 702, 1763
BfmI CTRYAG 2 cut(s) 708, 1215
BfuAI ACCTGC 1 cut(s) 14
BfuI GTATCC 1 cut(s) 1395
BlnI CCTAGG 1 cut(s) 499
BmcAI AGTACT 1 cut(s) 27
Bme1390I CCNGG 6 cut(s) 68, 982, 1133, 1599, 1889, 1890
Bme18I GGWCC 3 cut(s) 1019, 1550, 1787
BmeT110I CYCGRG 2 cut(s) 1545, 1888
BmgT120I GGNCC 5 cut(s) 503, 745, 1019, 1550, 1787
BmiI GGNNCC 5 cut(s) 718, 775, 964, 1435, 1551
BmrFI CCNGG 6 cut(s) 68, 982, 1133, 1599, 1889, 1890
BmrI ACTGGG 1 cut(s) 408
BmsI GCATC 4 cut(s) 664, 1016, 1636, 1735
BmtI GCTAGC 1 cut(s) 705
BmuI ACTGGG 1 cut(s) 408
BpmI CTGGAG 2 cut(s) 1215, 1725
Bpu10I CCTNAGC 1 cut(s) 835
Bpu14I TTCGAA 1 cut(s) 1493
BpuEI CTTGAG 2 cut(s) 1178, 1562
BpuMI CCSGG 2 cut(s) 1889, 1890
Bsa29I ATCGAT 2 cut(s) 77, 1676
BsaBI GATNNNNATC 1 cut(s) 171
BsaI GGTCTC 2 cut(s) 1743, 1999
BsaWI WCCGGW 2 cut(s) 539, 1186
BsaXI ACNNNNNCTCC 4 cut(s) 873, 903, 1771, 1801
Bsc4I CCNNNNNNNGG 5 cut(s) 523, 1193, 1376, 1968, 2138
Bse1I ACTGG 5 cut(s) 414, 506, 524, 964, 1739
Bse3DI GCAATG 3 cut(s) 560, 565, 951
Bse8I GATNNNNATC 1 cut(s) 171
BseAI TCCGGA 1 cut(s) 1186
BseBI CCWGG 4 cut(s) 68, 982, 1133, 1599
BseCI ATCGAT 2 cut(s) 77, 1676
BseGI GGATG 5 cut(s) 512, 937, 943, 1651, 1726
BseJI GATNNNNATC 1 cut(s) 171
BseLI CCNNNNNNNGG 5 cut(s) 523, 1193, 1376, 1968, 2138
BseMI GCAATG 3 cut(s) 560, 565, 951
BseMII CTCAG 7 cut(s) 401, 471, 609, 849, 1680, 1710, 2068
BseNI ACTGG 5 cut(s) 414, 506, 524, 964, 1739
BseRI GAGGAG 4 cut(s) 375, 1677, 1703, 1812
BseSI GKGCMC 2 cut(s) 778, 1869
BseYI CCCAGC 1 cut(s) 1649
BsgI GTGCAG 3 cut(s) 318, 1023, 1932
Bsh1236I CGCG 1 cut(s) 92
Bsh1285I CGRYCG 1 cut(s) 1294
BshNI GGYRCC 2 cut(s) 773, 1433
BshVI ATCGAT 2 cut(s) 77, 1676
BsiEI CGRYCG 1 cut(s) 1294
BsiHKAI GWGCWC 2 cut(s) 592, 1869
BsiHKCI CYCGRG 2 cut(s) 1545, 1888
BsiSI CCGG 3 cut(s) 540, 1187, 1889
BslFI GGGAC 4 cut(s) 649, 772, 1563, 1980
BslI CCNNNNNNNGG 5 cut(s) 523, 1193, 1376, 1968, 2138
BsmAI GTCTC 4 cut(s) 1699, 1743, 1999, 2091
BsmFI GGGAC 4 cut(s) 649, 772, 1563, 1980
BsmI GAATGC 2 cut(s) 1633, 1879
Bso31I GGTCTC 2 cut(s) 1743, 1999
BsoBI CYCGRG 2 cut(s) 1545, 1888
Bsp119I TTCGAA 1 cut(s) 1493
Bsp1286I GDGCHC 3 cut(s) 592, 778, 1869
Bsp13I TCCGGA 1 cut(s) 1186
Bsp143I GATC 7 cut(s) 159, 1056, 1084, 1457, 1754, 1817, 2128
Bsp19I CCATGG 1 cut(s) 1251
BspACI CCGC 6 cut(s) 90, 92, 632, 1341, 1505, 1560
BspCNI CTCAG 7 cut(s) 400, 470, 610, 848, 1679, 1709, 2067
BspDI ATCGAT 2 cut(s) 77, 1676
BspEI TCCGGA 1 cut(s) 1186
BspFNI CGCG 1 cut(s) 92
BspHI TCATGA 1 cut(s) 175
BspLI GGNNCC 5 cut(s) 718, 775, 964, 1435, 1551
BspMAI CTGCAG 1 cut(s) 712
BspMI ACCTGC 1 cut(s) 14
BspOI GCTAGC 1 cut(s) 705
BspPI GGATC 4 cut(s) 1064, 1092, 1825, 2123
BspT104I TTCGAA 1 cut(s) 1493
BspT107I GGYRCC 2 cut(s) 773, 1433
BspTNI GGTCTC 2 cut(s) 1743, 1999
BsrDI GCAATG 3 cut(s) 560, 565, 951
BsrI ACTGG 5 cut(s) 414, 506, 524, 964, 1739
BssMI GATC 7 cut(s) 159, 1056, 1084, 1457, 1754, 1817, 2128
BssNAI GTATAC 1 cut(s) 232
BssT1I CCWWGG 5 cut(s) 499, 748, 1052, 1251, 1524
Bst1107I GTATAC 1 cut(s) 232
Bst2UI CCWGG 4 cut(s) 68, 982, 1133, 1599
BstBI TTCGAA 1 cut(s) 1493
BstC8I GCNNGC 8 cut(s) 186, 285, 703, 1075, 1339, 1507, 1511, 2283
BstDEI CTNAG 8 cut(s) 96, 387, 457, 618, 835, 1666, 1696, 2054
BstDSI CCRYGG 4 cut(s) 90, 1080, 1251, 2184
BstF5I GGATG 5 cut(s) 512, 937, 943, 1651, 1726
BstFNI CGCG 1 cut(s) 92
BstHHI GCGC 3 cut(s) 283, 1030, 1597
BstKTI GATC 7 cut(s) 162, 1059, 1087, 1460, 1757, 1820, 2131
BstMAI GTCTC 4 cut(s) 1699, 1743, 1999, 2091
BstMBI GATC 7 cut(s) 159, 1056, 1084, 1457, 1754, 1817, 2128
BstMCI CGRYCG 1 cut(s) 1294
BstMWI GCNNNNNNNGC 7 cut(s) 707, 782, 791, 1322, 1347, 1529, 1568
BstNI CCWGG 4 cut(s) 68, 982, 1133, 1599
BstNSI RCATGY 2 cut(s) 1995, 2285
BstSCI CCNGG 6 cut(s) 66, 980, 1131, 1597, 1887, 1888
BstSFI CTRYAG 2 cut(s) 708, 1215
BstSLI GKGCMC 2 cut(s) 778, 1869
BstUI CGCG 1 cut(s) 92
BstX2I RGATCY 1 cut(s) 1817
BstXI CCANNNNNNTGG 2 cut(s) 519, 2170
BstYI RGATCY 1 cut(s) 1817
BstZ17I GTATAC 1 cut(s) 232
Bsu15I ATCGAT 2 cut(s) 77, 1676
BsuI GTATCC 1 cut(s) 1395
BsuTUI ATCGAT 2 cut(s) 77, 1676
BtgI CCRYGG 4 cut(s) 90, 1080, 1251, 2184
BtgZI GCGATG 1 cut(s) 1955
BtsCI GGATG 5 cut(s) 512, 937, 943, 1651, 1726
BtsI GCAGTG 3 cut(s) 705, 1628, 1721
BtsIMutI CAGTG 5 cut(s) 90, 517, 705, 1628, 1721
BveI ACCTGC 1 cut(s) 14
Cac8I GCNNGC 8 cut(s) 186, 285, 703, 1075, 1339, 1507, 1511, 2283
CaiI CAGNNNCTG 3 cut(s) 634, 842, 1325
CciI TCATGA 1 cut(s) 175
CfoI GCGC 3 cut(s) 283, 1030, 1597
Cfr13I GGNCC 5 cut(s) 503, 745, 1019, 1550, 1787
Cfr42I CCGCGG 1 cut(s) 93
Cfr9I CCCGGG 1 cut(s) 1888
ClaI ATCGAT 2 cut(s) 77, 1676
Csp6I GTAC 2 cut(s) 26, 2245
CspCI CAANNNNNGTGG 2 cut(s) 178, 213
CviQI GTAC 2 cut(s) 26, 2245
DdeI CTNAG 8 cut(s) 96, 387, 457, 618, 835, 1666, 1696, 2054
DpnI GATC 7 cut(s) 161, 1058, 1086, 1459, 1756, 1819, 2130
DpnII GATC 7 cut(s) 159, 1056, 1084, 1457, 1754, 1817, 2128
DraIII CACNNNGTG 1 cut(s) 121
EaeI YGGCCR 4 cut(s) 87, 467, 792, 978
Eco130I CCWWGG 5 cut(s) 499, 748, 1052, 1251, 1524
Eco147I AGGCCT 1 cut(s) 498
Eco31I GGTCTC 2 cut(s) 1743, 1999
Eco32I GATATC 1 cut(s) 1469
Eco47I GGWCC 3 cut(s) 1019, 1550, 1787
Eco57I CTGAAG 1 cut(s) 1302
Eco88I CYCGRG 2 cut(s) 1545, 1888
EcoO109I RGGNCCY 1 cut(s) 745
EcoRI GAATTC 2 cut(s) 656, 2292
EcoRII CCWGG 4 cut(s) 66, 980, 1131, 1597
EcoRV GATATC 1 cut(s) 1469
EcoT14I CCWWGG 5 cut(s) 499, 748, 1052, 1251, 1524
ErhI CCWWGG 5 cut(s) 499, 748, 1052, 1251, 1524
FaqI GGGAC 4 cut(s) 649, 772, 1563, 1980
FauI CCCGC 1 cut(s) 1498
FbaI TGATCA 1 cut(s) 159
FblI GTMKAC 1 cut(s) 231
FokI GGATG 5 cut(s) 499, 944, 950, 1658, 1713
FspBI CTAG 4 cut(s) 357, 500, 702, 1763
GlaI GCGC 3 cut(s) 282, 1029, 1596
GsaI CCCAGC 1 cut(s) 1653
GsuI CTGGAG 2 cut(s) 1215, 1725
HapII CCGG 3 cut(s) 540, 1187, 1889
HhaI GCGC 3 cut(s) 283, 1030, 1597
Hin6I GCGC 3 cut(s) 281, 1028, 1595
HinP1I GCGC 3 cut(s) 281, 1028, 1595
HincII GTYRAC 2 cut(s) 1420, 1833
HindII GTYRAC 2 cut(s) 1420, 1833
HindIII AAGCTT 3 cut(s) 269, 855, 1174
HinfI GANTC 9 cut(s) 167, 321, 455, 473, 536, 1190, 1229, 1366, 2099
HpaII CCGG 3 cut(s) 540, 1187, 1889
HphI GGTGA 4 cut(s) 384, 440, 554, 1041
Hpy166II GTNNAC 8 cut(s) 115, 232, 1149, 1420, 1606, 1799, 1833, 1867
Hpy188I TCNGA 8 cut(s) 60, 220, 646, 931, 1041, 1457, 2057, 2193
Hpy8I GTNNAC 8 cut(s) 115, 232, 1149, 1420, 1606, 1799, 1833, 1867
HpyAV CCTTC 7 cut(s) 56, 405, 488, 517, 1032, 2019, 2043
HpyCH4IV ACGT 2 cut(s) 592, 943
HpyF10VI GCNNNNNNNGC 7 cut(s) 707, 782, 791, 1322, 1347, 1529, 1568
HpyF3I CTNAG 8 cut(s) 96, 387, 457, 618, 835, 1666, 1696, 2054
HpySE526I ACGT 2 cut(s) 592, 943
HspAI GCGC 3 cut(s) 281, 1028, 1595
Kpn2I TCCGGA 1 cut(s) 1186
Ksp22I TGATCA 1 cut(s) 159
KspI CCGCGG 1 cut(s) 93
Kzo9I GATC 7 cut(s) 159, 1056, 1084, 1457, 1754, 1817, 2128
LmnI GCTCC 3 cut(s) 587, 839, 968
LweI GCATC 4 cut(s) 664, 1016, 1636, 1735
MaeI CTAG 4 cut(s) 357, 500, 702, 1763
MaeII ACGT 2 cut(s) 592, 943
MaeIII GTNAC 9 cut(s) 236, 390, 724, 737, 894, 1306, 1765, 2071, 2083
MalI GATC 7 cut(s) 161, 1058, 1086, 1459, 1756, 1819, 2130
MboI GATC 7 cut(s) 159, 1056, 1084, 1457, 1754, 1817, 2128
MboII GAAGA 2 cut(s) 401, 503
MfeI CAATTG 2 cut(s) 1500, 2124
MflI RGATCY 1 cut(s) 1817
MhlI GDGCHC 3 cut(s) 592, 778, 1869
MlsI TGGCCA 3 cut(s) 469, 794, 980
MluNI TGGCCA 3 cut(s) 469, 794, 980
MlyI GAGTC 5 cut(s) 330, 449, 482, 1199, 2093
MmeI TCCRAC 2 cut(s) 178, 1394
Mox20I TGGCCA 3 cut(s) 469, 794, 980
MroI TCCGGA 1 cut(s) 1186
MscI TGGCCA 3 cut(s) 469, 794, 980
MseI TTAA 3 cut(s) 552, 1242, 2025
MslI CAYNNNNRTG 2 cut(s) 684, 1872
Msp20I TGGCCA 3 cut(s) 469, 794, 980
MspA1I CMGCKG 4 cut(s) 92, 634, 713, 1322
MspI CCGG 3 cut(s) 540, 1187, 1889
MspR9I CCNGG 6 cut(s) 68, 982, 1133, 1599, 1889, 1890
MunI CAATTG 2 cut(s) 1500, 2124
Mva1269I GAATGC 2 cut(s) 1633, 1879
MvaI CCWGG 4 cut(s) 68, 982, 1133, 1599
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 7 cut(s) 707, 782, 791, 1322, 1347, 1529, 1568
NciI CCSGG 2 cut(s) 1889, 1890
NcoI CCATGG 1 cut(s) 1251
NdeII GATC 7 cut(s) 159, 1056, 1084, 1457, 1754, 1817, 2128
NheI GCTAGC 1 cut(s) 701
NlaIV GGNNCC 5 cut(s) 718, 775, 964, 1435, 1551
NmeAIII GCCGAG 1 cut(s) 1837
NmuCI GTSAC 5 cut(s) 390, 724, 894, 1765, 2083
NspI RCATGY 2 cut(s) 1995, 2285
NspV TTCGAA 1 cut(s) 1493
PaeI GCATGC 1 cut(s) 2285
PagI TCATGA 1 cut(s) 175
PceI AGGCCT 1 cut(s) 498
PciI ACATGT 1 cut(s) 1991
PctI GAATGC 2 cut(s) 1633, 1879
PfeI GAWTC 4 cut(s) 167, 536, 1229, 1366
PflMI CCANNNNNTGG 1 cut(s) 1376
PleI GAGTC 5 cut(s) 329, 449, 481, 1198, 2093
PpsI GAGTC 5 cut(s) 329, 449, 481, 1198, 2093
PscI ACATGT 1 cut(s) 1991
Psp6I CCWGG 4 cut(s) 66, 980, 1131, 1597
PspFI CCCAGC 1 cut(s) 1649
PspGI CCWGG 4 cut(s) 66, 980, 1131, 1597
PspN4I GGNNCC 5 cut(s) 718, 775, 964, 1435, 1551
PspPI GGNCC 5 cut(s) 503, 745, 1019, 1550, 1787
PsrI GAACNNNNNNTAC 2 cut(s) 2228, 2260
PstI CTGCAG 1 cut(s) 712
PstNI CAGNNNCTG 3 cut(s) 634, 842, 1325
PsuI RGATCY 1 cut(s) 1817
PvuII CAGCTG 2 cut(s) 713, 1322
RsaI GTAC 2 cut(s) 27, 2246
RsaNI GTAC 2 cut(s) 26, 2245
RseI CAYNNNNRTG 2 cut(s) 684, 1872
SacII CCGCGG 1 cut(s) 93
SaqAI TTAA 3 cut(s) 552, 1242, 2025
Sau3AI GATC 7 cut(s) 159, 1056, 1084, 1457, 1754, 1817, 2128
Sau96I GGNCC 5 cut(s) 503, 745, 1019, 1550, 1787
ScaI AGTACT 1 cut(s) 27
SchI GAGTC 5 cut(s) 330, 449, 482, 1199, 2093
ScrFI CCNGG 6 cut(s) 68, 982, 1133, 1599, 1889, 1890
SduI GDGCHC 3 cut(s) 592, 778, 1869
SfaNI GCATC 4 cut(s) 664, 1016, 1636, 1735
SfcI CTRYAG 2 cut(s) 708, 1215
Sfr303I CCGCGG 1 cut(s) 93
SfuI TTCGAA 1 cut(s) 1493
SgrBI CCGCGG 1 cut(s) 93
SinI GGWCC 3 cut(s) 1019, 1550, 1787
SmaI CCCGGG 1 cut(s) 1890
SmiMI CAYNNNNRTG 2 cut(s) 684, 1872
SmlI CTYRAG 2 cut(s) 1157, 1577
SmoI CTYRAG 2 cut(s) 1157, 1577
SpeI ACTAGT 1 cut(s) 1762
SphI GCATGC 1 cut(s) 2285
SseBI AGGCCT 1 cut(s) 498
SsiI CCGC 6 cut(s) 90, 92, 632, 1341, 1505, 1560
SspI AATATT 2 cut(s) 406, 1518
SspMI CTAG 4 cut(s) 357, 500, 702, 1763
StuI AGGCCT 1 cut(s) 498
StyD4I CCNGG 6 cut(s) 66, 980, 1131, 1597, 1887, 1888
StyI CCWWGG 5 cut(s) 499, 748, 1052, 1251, 1524
TaiI ACGT 2 cut(s) 595, 946
TaqII GACCGA 1 cut(s) 1280
TatI WGTACW 1 cut(s) 25
TauI GCSGC 3 cut(s) 92, 95, 634
TfiI GAWTC 4 cut(s) 167, 536, 1229, 1366
Tru1I TTAA 3 cut(s) 552, 1242, 2025
Tru9I TTAA 3 cut(s) 552, 1242, 2025
TscAI CASTG 5 cut(s) 90, 524, 712, 1635, 1728
TseFI GTSAC 5 cut(s) 390, 724, 894, 1765, 2083
Tsp45I GTSAC 5 cut(s) 390, 724, 894, 1765, 2083
TspDTI ATGAA 9 cut(s) 504, 649, 879, 954, 959, 1530, 1626, 2130, 2249
TspGWI ACGGA 4 cut(s) 179, 290, 1097, 2098
TspMI CCCGGG 1 cut(s) 1888
TspRI CASTG 5 cut(s) 90, 524, 712, 1635, 1728
Van91I CCANNNNNTGG 1 cut(s) 1376
VneI GTGCAC 1 cut(s) 1865
VpaK11BI GGWCC 3 cut(s) 1019, 1550, 1787
XapI RAATTY 3 cut(s) 548, 656, 2292
XceI RCATGY 2 cut(s) 1995, 2285
XcmI CCANNNNNNNNNTGG 1 cut(s) 421
XmaI CCCGGG 1 cut(s) 1888
XmaJI CCTAGG 1 cut(s) 499
XmiI GTMKAC 1 cut(s) 231
XspI CTAG 4 cut(s) 357, 500, 702, 1763
ZrmI AGTACT 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.