pycom14g11440

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0001127
Physical Location & Seq
Forward (+)
14523069 .. 14526507
3439 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g11440.7

Sequence Viewer

Length: 2295 bp
ATGAGCAGCTCAAGTTCAATACTCGCTCTACTCAGCCTTATCTGTTTTACTGGTCAGGGATTGATAACAAAGGTGGCAGCAAAGAGCCATGTACACATTGTTTACATGGGAGATAGGCAGCATGACAACACCAAGTTAATCACAGATTCACATCATGACTTGCTTGCCACTGTCGTGGGAAGCAGGGAATTAGCCTCGGAATTGATGGTATACAGTTACAAACATGGTTTCTCTGGGTTTGCAGCCAAGCTCACAGAGTCTCAAACCCAACAACTTTCAGAGTTGCCTGGTGTTGTACGAATCATACCAAATAGCCTTCATAAGCTGGAAACAACTAGGAGTTGGGATTTTCTTGGCCTCTCACCTCACTCCCCTTCCAATATTTTTCCCAAGAGCAACATGGGGGATGGAGTTATCATAGGTGTTCTTGACACAGGTATATGGCCGGAGTCTGAATCATTCAATGAAAAAGGCCTCGGGCCTGTTCCATCTCATTGGAATGGTGTTTGTGAATCCGGGGACAATTTCAATGCTACAATACATTGCAATAGAAAAATCATCGGAGCCCGTTGGTTTATCAATGGACTTCTTGCCGAGTATGCAAAGCCATTGGACAAAGAATTCCATTCTCCTAGAGATGCACATGGACACGGTACTCATACCTCAAGCACTGCAGCTGGTTCTTTTGTGGCTGATATCAGCTACAAGGGCCTTGGTCTTGGTACAATTAGAGGCGGTGCTCCAAATGCTCGGTTGGCCATTTACAAGGTTTGTTGGAATGTGCTTGGAGGCAAATGCTCAACAGCTGATATGTTGAAAGCTTTTGACGAAGCAATACATGATGGGGTTGATGTGCTGTCAGTGTCAATTGGGTATCCTATTCCCCTCTTCTCAGATGTTGATGAGCGTGATGGCATTGCAACCGGTTCCTTTCATGCTGTGGCTAAAGGGATTACTGTTGTGTGCTCAGCTGGAAATAATGGACCTTCAGCACAAACAGTTTCGAAGACAGCACCGTGGATCATAACTGTTGCAGCAAGCACCATGGATCGAGAATTTCCTACTTCTATAACTTTAGGAAATAATAAAACTTTCCTGGTATGTGGTCAAGCTATGTTTATAGGGACAGAGATTGGATTTACAAGCTTGGTATACCCAGAGTCTAAAGGGCTTACCACCAGAGGTGTGTGTGAATCTCTCTCACTTAACAAAACTATAGTAGTTGGAAAGGTGGTTCTCTGCTTCACTACGATGGGTCGTCAGGCGATAACAAATGCTTCAGCAGTTGTGAAAGAAGCAGGGGGTGCTGGCCTAATCATCGCAAAGAATCCAAATGATGCTTTATATCCATGTAATGAAGACTTTCCATGCATTGAAGTTGACTATGAGATTGGTACTCGAATTCTATTTTATATCCGGTCTACCAGATATCCTCTTGTAAAGCTGAGCCCTCCTAAAACAATTGTGGGCAAGCCACTATCTGCCAAGGTGGCTTCTTTTTCCTCTAGGGGACCAAACTCCATAACACCAGCAATTCTCAAGCCAGATATAGCCGCGCCTGGTGTGAACATACTAGCAGCAACCTCTCCACTTTATTCATTCGCGGAGGGTGGATATGCCATGATGTCTGGAACATCAATGTCAAGTCCTCATGTCACAGGCATTGTGGCTCTTATCAAGAGAATGCATCCTAATTGGTCTCCAGCAGCCATCAAATTAGCACTAGAACGGCCCATCTGGTTTACCGATATTTGCAGAAGGATCGATCCTCAAAAGTTGGCAAATTCATTTGACTTTGGAGGTGGTCTGGTGAATCCCAATGGCGCAGCAGACCCTGGACTAGTATACGACATGGGTGCAGCAGACTACATGGAATATCTTTGTGCAAGGGGGTACAACAACTCTGCCATCTCTCGACTTACAGGGAAAAACACAACATGTCCCGTCAAGAAGCCCTCTATCCTTGACGTTAACCTACCTTCCGTGACCATACCTAGTCTGAGAAATCCAATCACAGTAAAAAGAACTGTAACAAATGTTGGAGCCCCTGAGTCCATTTACAAAGCTACAATTGAACCTCCATTCGGTACAATTGTCTATGTGAACCCTACTGCTCTGGTCTTCAATTCCACAGTTGAAAAACTCACTTTCACAATCACAATCTCTGCAATCCACAAGATGAACACTGGTTACTATTTTGGAAGCCTTACTTGGGTTGACGGAGTGCATGCCGTGAGGATTCCTTTGTCTGTGAGAACTGAGTTCTTACAATCTCATGATGATGATGAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

765

Amino Acids

81.91

Weight (kDa)

7.58

Isoelectric Point (pI)

34.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 4 cut(s) 210, 1152, 1421, 1845
AccII CGCG 2 cut(s) 1556, 1604
AciI CCGC 3 cut(s) 735, 1554, 1604
AclWI GGATC 4 cut(s) 1028, 1056, 1760, 1769
AcoI YGGCCR 2 cut(s) 443, 756
AcsI RAATTY 4 cut(s) 620, 1055, 1401, 1783
AcuI CTGAAG 2 cut(s) 972, 1263
AfaI GTAC 7 cut(s) 93, 297, 655, 724, 1396, 1895, 2089
AfiI CCNNNNNNNGG 2 cut(s) 2084, 2212
AflIII ACRYGT 1 cut(s) 1937
AgeI ACCGGT 1 cut(s) 923
AgsI TTSAA 8 cut(s) 18, 463, 529, 817, 1376, 2075, 2125, 2138
AhlI ACTAGT 1 cut(s) 1840
AjnI CCWGG 4 cut(s) 286, 1095, 1558, 1834
AjuI GAANNNNNNNTTGG 2 cut(s) 2194, 2226
AleI CACNNNNGTG 1 cut(s) 173
AloI GAACNNNNNNTCC 2 cut(s) 1218, 1250
Alw21I GWGCWC 2 cut(s) 742, 968
Alw26I GTCTC 2 cut(s) 264, 1704
AlwI GGATC 4 cut(s) 1028, 1056, 1760, 1769
AlwNI CAGNNNCTG 1 cut(s) 1835
Ama87I CYCGRG 1 cut(s) 476
AoxI GGCC 8 cut(s) 355, 443, 472, 479, 709, 756, 1309, 1730
ApoI RAATTY 4 cut(s) 620, 1055, 1401, 1783
AsiGI ACCGGT 1 cut(s) 923
Asp700I GAANNNNTTC 1 cut(s) 1362
AspLEI GCGC 2 cut(s) 1558, 1826
AspS9I GGNCC 5 cut(s) 479, 709, 983, 1511, 1731
AsuC2I CCSGG 1 cut(s) 517
AsuHPI GGTGA 2 cut(s) 354, 1822
AsuII TTCGAA 1 cut(s) 1004
AvaI CYCGRG 1 cut(s) 476
AvaII GGWCC 2 cut(s) 983, 1511
BaeI ACNNNNGTAYC 2 cut(s) 2079, 2112
BalI TGGCCA 1 cut(s) 758
BanII GRGCYC 3 cut(s) 568, 1451, 2047
BbsI GAAGAC 3 cut(s) 1013, 1365, 2113
Bbv12I GWGCWC 2 cut(s) 742, 968
BccI CCATC 9 cut(s) 199, 401, 496, 836, 905, 1246, 1718, 1742, 1916
BceAI ACGGC 2 cut(s) 1745, 2216
BcgI CGANNNNNNTGC 4 cut(s) 1744, 1778, 1838, 1872
BciT130I CCWGG 4 cut(s) 288, 1097, 1560, 1836
BciVI GTATCC 1 cut(s) 885
BcnI CCSGG 1 cut(s) 517
BcoDI GTCTC 2 cut(s) 264, 1704
BcuI ACTAGT 1 cut(s) 1840
BfaI CTAG 7 cut(s) 336, 633, 1506, 1574, 1724, 1841, 1995
BfmI CTRYAG 2 cut(s) 672, 1215
BfuI GTATCC 1 cut(s) 885
BglI GCCNNNNNGGC 1 cut(s) 1490
BlpI GCTNAGC 2 cut(s) 967, 1445
Bme1390I CCNGG 5 cut(s) 288, 517, 1097, 1560, 1836
Bme18I GGWCC 2 cut(s) 983, 1511
BmeT110I CYCGRG 1 cut(s) 476
BmgT120I GGNCC 5 cut(s) 479, 709, 983, 1511, 1731
BmiI GGNNCC 4 cut(s) 565, 928, 1512, 2044
BmrFI CCNGG 5 cut(s) 288, 517, 1097, 1560, 1836
BmsI GCATC 3 cut(s) 628, 1327, 1696
BpiI GAAGAC 3 cut(s) 1013, 1365, 2113
BpmI CTGGAG 1 cut(s) 1686
Bpu1102I GCTNAGC 2 cut(s) 967, 1445
Bpu14I TTCGAA 1 cut(s) 1004
BpuEI CTTGAG 2 cut(s) 649, 1523
BpuMI CCSGG 1 cut(s) 517
Bsa29I ATCGAT 1 cut(s) 1764
BsaBI GATNNNNATC 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 1704
BsaJI CCNNGG 8 cut(s) 195, 475, 516, 712, 1016, 1044, 1485, 1834
BsaWI WCCGGW 2 cut(s) 923, 1416
Bsc4I CCNNNNNNNGG 2 cut(s) 2084, 2212
Bse118I RCCGGY 1 cut(s) 923
Bse1I ACTGG 2 cut(s) 55, 2191
Bse3DI GCAATG 2 cut(s) 541, 915
Bse8I GATNNNNATC 1 cut(s) 150
BseBI CCWGG 4 cut(s) 288, 1097, 1560, 1836
BseCI ATCGAT 1 cut(s) 1764
BseDI CCNNGG 8 cut(s) 195, 475, 516, 712, 1016, 1044, 1485, 1834
BseGI GGATG 2 cut(s) 412, 1687
BseJI GATNNNNATC 1 cut(s) 150
BseLI CCNNNNNNNGG 2 cut(s) 2084, 2212
BseMI GCAATG 2 cut(s) 541, 915
BseMII CTCAG 7 cut(s) 46, 906, 981, 1436, 1991, 2040, 2250
BseNI ACTGG 2 cut(s) 55, 2191
BsgI GTGCAG 1 cut(s) 1878
Bsh1236I CGCG 2 cut(s) 1556, 1604
BshFI GGCC 8 cut(s) 357, 445, 474, 481, 711, 758, 1311, 1732
BshTI ACCGGT 1 cut(s) 923
BshVI ATCGAT 1 cut(s) 1764
BsiHKAI GWGCWC 2 cut(s) 742, 968
BsiHKCI CYCGRG 1 cut(s) 476
BsiSI CCGG 4 cut(s) 446, 516, 924, 1417
BslFI GGGAC 4 cut(s) 533, 1138, 1524, 1926
BslI CCNNNNNNNGG 2 cut(s) 2084, 2212
BsmAI GTCTC 2 cut(s) 264, 1704
BsmFI GGGAC 4 cut(s) 533, 1138, 1524, 1926
BsmI GAATGC 1 cut(s) 1689
BsnI GGCC 8 cut(s) 357, 445, 474, 481, 711, 758, 1311, 1732
Bso31I GGTCTC 1 cut(s) 1704
BsoBI CYCGRG 1 cut(s) 476
Bsp119I TTCGAA 1 cut(s) 1004
Bsp1286I GDGCHC 5 cut(s) 568, 742, 968, 1451, 2047
Bsp1407I TGTACA 1 cut(s) 91
Bsp143I GATC 4 cut(s) 1020, 1048, 1761, 1765
Bsp1720I GCTNAGC 2 cut(s) 967, 1445
Bsp19I CCATGG 1 cut(s) 1044
BspACI CCGC 3 cut(s) 735, 1554, 1604
BspANI GGCC 8 cut(s) 357, 445, 474, 481, 711, 758, 1311, 1732
BspCNI CTCAG 7 cut(s) 45, 905, 980, 1437, 1992, 2041, 2251
BspDI ATCGAT 1 cut(s) 1764
BspFNI CGCG 2 cut(s) 1556, 1604
BspHI TCATGA 2 cut(s) 154, 2275
BspLI GGNNCC 4 cut(s) 565, 928, 1512, 2044
BspMAI CTGCAG 1 cut(s) 676
BspPI GGATC 4 cut(s) 1028, 1056, 1760, 1769
BspT104I TTCGAA 1 cut(s) 1004
BspTNI GGTCTC 1 cut(s) 1704
BsrDI GCAATG 2 cut(s) 541, 915
BsrFI RCCGGY 1 cut(s) 923
BsrGI TGTACA 1 cut(s) 91
BsrI ACTGG 2 cut(s) 55, 2191
BssAI RCCGGY 1 cut(s) 923
BssECI CCNNGG 8 cut(s) 195, 475, 516, 712, 1016, 1044, 1485, 1834
BssMI GATC 4 cut(s) 1020, 1048, 1761, 1765
BssNAI GTATAC 3 cut(s) 211, 1153, 1846
BssT1I CCWWGG 3 cut(s) 712, 1044, 1485
Bst1107I GTATAC 3 cut(s) 211, 1153, 1846
Bst2UI CCWGG 4 cut(s) 288, 1097, 1560, 1836
Bst6I CTCTTC 1 cut(s) 893
BstAPI GCANNNNNTGC 1 cut(s) 1304
BstAUI TGTACA 1 cut(s) 91
BstBI TTCGAA 1 cut(s) 1004
BstC8I GCNNGC 5 cut(s) 165, 1039, 1309, 1472, 2229
BstDEI CTNAG 7 cut(s) 32, 892, 967, 1445, 2000, 2049, 2259
BstDSI CCRYGG 2 cut(s) 1016, 1044
BstF5I GGATG 2 cut(s) 412, 1687
BstFNI CGCG 2 cut(s) 1556, 1604
BstHHI GCGC 2 cut(s) 1558, 1826
BstKTI GATC 4 cut(s) 1023, 1051, 1764, 1768
BstMAI GTCTC 2 cut(s) 264, 1704
BstMBI GATC 4 cut(s) 1020, 1048, 1761, 1765
BstMWI GCNNNNNNNGC 6 cut(s) 599, 708, 746, 755, 1304, 1490
BstNI CCWGG 4 cut(s) 288, 1097, 1560, 1836
BstNSI RCATGY 2 cut(s) 1941, 2231
BstSCI CCNGG 5 cut(s) 286, 515, 1095, 1558, 1834
BstSFI CTRYAG 2 cut(s) 672, 1215
BstUI CGCG 2 cut(s) 1556, 1604
BstV2I GAAGAC 3 cut(s) 1013, 1365, 2113
BstXI CCANNNNNNTGG 2 cut(s) 175, 495
BstZ17I GTATAC 3 cut(s) 211, 1153, 1846
Bsu15I ATCGAT 1 cut(s) 1764
BsuI GTATCC 1 cut(s) 885
BsuRI GGCC 8 cut(s) 357, 445, 474, 481, 711, 758, 1311, 1732
BsuTUI ATCGAT 1 cut(s) 1764
BtgI CCRYGG 2 cut(s) 1016, 1044
BtgZI GCGATG 1 cut(s) 1303
BtsCI GGATG 2 cut(s) 412, 1687
BtsI GCAGTG 1 cut(s) 669
BtsIMutI CAGTG 4 cut(s) 168, 669, 867, 2184
Cac8I GCNNGC 5 cut(s) 165, 1039, 1309, 1472, 2229
CaiI CAGNNNCTG 1 cut(s) 1835
CciI TCATGA 2 cut(s) 154, 2275
CfoI GCGC 2 cut(s) 1558, 1826
Cfr10I RCCGGY 1 cut(s) 923
Cfr13I GGNCC 5 cut(s) 479, 709, 983, 1511, 1731
ClaI ATCGAT 1 cut(s) 1764
Csp6I GTAC 7 cut(s) 92, 296, 654, 723, 1395, 1894, 2088
CspAI ACCGGT 1 cut(s) 923
CviQI GTAC 7 cut(s) 92, 296, 654, 723, 1395, 1894, 2088
DdeI CTNAG 7 cut(s) 32, 892, 967, 1445, 2000, 2049, 2259
DpnI GATC 4 cut(s) 1022, 1050, 1763, 1767
DpnII GATC 4 cut(s) 1020, 1048, 1761, 1765
EaeI YGGCCR 2 cut(s) 443, 756
Eam1104I CTCTTC 1 cut(s) 893
EarI CTCTTC 1 cut(s) 893
Eco130I CCWWGG 3 cut(s) 712, 1044, 1485
Eco147I AGGCCT 1 cut(s) 474
Eco24I GRGCYC 3 cut(s) 568, 1451, 2047
Eco31I GGTCTC 1 cut(s) 1704
Eco32I GATATC 2 cut(s) 697, 1430
Eco47I GGWCC 2 cut(s) 983, 1511
Eco57I CTGAAG 2 cut(s) 972, 1263
Eco88I CYCGRG 1 cut(s) 476
EcoO109I RGGNCCY 1 cut(s) 709
EcoRI GAATTC 2 cut(s) 620, 1401
EcoRII CCWGG 4 cut(s) 286, 1095, 1558, 1834
EcoRV GATATC 2 cut(s) 697, 1430
EcoT14I CCWWGG 3 cut(s) 712, 1044, 1485
EcoT22I ATGCAT 2 cut(s) 1373, 1689
EcoT38I GRGCYC 3 cut(s) 568, 1451, 2047
ErhI CCWWGG 3 cut(s) 712, 1044, 1485
FalI AAGNNNNNCTT 2 cut(s) 2194, 2226
FaqI GGGAC 4 cut(s) 533, 1138, 1524, 1926
FblI GTMKAC 4 cut(s) 210, 1152, 1421, 1845
FokI GGATG 2 cut(s) 419, 1674
FriOI GRGCYC 3 cut(s) 568, 1451, 2047
FspBI CTAG 7 cut(s) 336, 633, 1506, 1574, 1724, 1841, 1995
GlaI GCGC 2 cut(s) 1557, 1825
GsuI CTGGAG 1 cut(s) 1686
HaeIII GGCC 8 cut(s) 357, 445, 474, 481, 711, 758, 1311, 1732
HapII CCGG 4 cut(s) 446, 516, 924, 1417
HhaI GCGC 2 cut(s) 1558, 1826
Hin6I GCGC 2 cut(s) 1556, 1824
HinP1I GCGC 2 cut(s) 1556, 1824
HincII GTYRAC 3 cut(s) 1381, 1972, 2218
HindII GTYRAC 3 cut(s) 1381, 1972, 2218
HindIII AAGCTT 2 cut(s) 819, 1144
HpaI GTTAAC 1 cut(s) 1972
HpaII CCGG 4 cut(s) 446, 516, 924, 1417
HphI GGTGA 2 cut(s) 354, 1822
Hpy188I TCNGA 6 cut(s) 199, 280, 454, 563, 895, 2001
Hpy188III TCNNGA 8 cut(s) 155, 428, 1052, 1629, 1678, 1914, 1948, 2276
HpyAV CCTTC 5 cut(s) 326, 384, 996, 1752, 1989
HpyCH4IV ACGT 1 cut(s) 1968
HpyF10VI GCNNNNNNNGC 6 cut(s) 599, 708, 746, 755, 1304, 1490
HpyF3I CTNAG 7 cut(s) 32, 892, 967, 1445, 2000, 2049, 2259
HpySE526I ACGT 1 cut(s) 1968
HspAI GCGC 2 cut(s) 1556, 1824
KspAI GTTAAC 1 cut(s) 1972
Kzo9I GATC 4 cut(s) 1020, 1048, 1761, 1765
LmnI GCTCC 3 cut(s) 563, 745, 2042
LweI GCATC 3 cut(s) 628, 1327, 1696
MaeI CTAG 7 cut(s) 336, 633, 1506, 1574, 1724, 1841, 1995
MaeII ACGT 1 cut(s) 1968
MaeIII GTNAC 5 cut(s) 215, 1654, 1984, 2029, 2189
MalI GATC 4 cut(s) 1022, 1050, 1763, 1767
MboI GATC 4 cut(s) 1020, 1048, 1761, 1765
MboII GAAGA 4 cut(s) 880, 1018, 1370, 2113
MfeI CAATTG 4 cut(s) 867, 1461, 2070, 2091
MhlI GDGCHC 5 cut(s) 568, 742, 968, 1451, 2047
MlsI TGGCCA 1 cut(s) 758
MluNI TGGCCA 1 cut(s) 758
MlyI GAGTC 4 cut(s) 266, 458, 1169, 2060
MmeI TCCRAC 3 cut(s) 755, 1204, 2020
Mox20I TGGCCA 1 cut(s) 758
Mph1103I ATGCAT 2 cut(s) 1373, 1689
MroXI GAANNNNTTC 1 cut(s) 1362
MscI TGGCCA 1 cut(s) 758
MseI TTAA 3 cut(s) 137, 1206, 1971
MslI CAYNNNNRTG 4 cut(s) 173, 498, 1250, 2280
Msp20I TGGCCA 1 cut(s) 758
MspA1I CMGCKG 3 cut(s) 677, 806, 971
MspI CCGG 4 cut(s) 446, 516, 924, 1417
MspR9I CCNGG 5 cut(s) 288, 517, 1097, 1560, 1836
MunI CAATTG 4 cut(s) 867, 1461, 2070, 2091
Mva1269I GAATGC 1 cut(s) 1689
MvaI CCWGG 4 cut(s) 288, 1097, 1560, 1836
MvnI CGCG 2 cut(s) 1556, 1604
MwoI GCNNNNNNNGC 6 cut(s) 599, 708, 746, 755, 1304, 1490
NciI CCSGG 1 cut(s) 517
NcoI CCATGG 1 cut(s) 1044
NdeII GATC 4 cut(s) 1020, 1048, 1761, 1765
NlaIV GGNNCC 4 cut(s) 565, 928, 1512, 2044
NmeAIII GCCGAG 1 cut(s) 619
NmuCI GTSAC 2 cut(s) 1654, 1984
NsiI ATGCAT 2 cut(s) 1373, 1689
NspI RCATGY 2 cut(s) 1941, 2231
NspV TTCGAA 1 cut(s) 1004
OliI CACNNNNGTG 1 cut(s) 173
PaeI GCATGC 1 cut(s) 2231
PagI TCATGA 2 cut(s) 154, 2275
PceI AGGCCT 1 cut(s) 474
PciI ACATGT 1 cut(s) 1937
PctI GAATGC 1 cut(s) 1689
PdmI GAANNNNTTC 1 cut(s) 1362
PfeI GAWTC 8 cut(s) 146, 300, 455, 512, 1193, 1327, 1813, 2239
PinAI ACCGGT 1 cut(s) 923
PleI GAGTC 4 cut(s) 265, 457, 1168, 2059
PpsI GAGTC 4 cut(s) 265, 457, 1168, 2059
PscI ACATGT 1 cut(s) 1937
Psp6I CCWGG 4 cut(s) 286, 1095, 1558, 1834
PspGI CCWGG 4 cut(s) 286, 1095, 1558, 1834
PspN4I GGNNCC 4 cut(s) 565, 928, 1512, 2044
PspPI GGNCC 5 cut(s) 479, 709, 983, 1511, 1731
PsrI GAACNNNNNNTAC 2 cut(s) 2174, 2206
PstI CTGCAG 1 cut(s) 676
PstNI CAGNNNCTG 1 cut(s) 1835
PvuII CAGCTG 3 cut(s) 677, 806, 971
RsaI GTAC 7 cut(s) 93, 297, 655, 724, 1396, 1895, 2089
RsaNI GTAC 7 cut(s) 92, 296, 654, 723, 1395, 1894, 2088
RseI CAYNNNNRTG 4 cut(s) 173, 498, 1250, 2280
SaqAI TTAA 3 cut(s) 137, 1206, 1971
Sau3AI GATC 4 cut(s) 1020, 1048, 1761, 1765
Sau96I GGNCC 5 cut(s) 479, 709, 983, 1511, 1731
SchI GAGTC 4 cut(s) 266, 458, 1169, 2060
ScrFI CCNGG 5 cut(s) 288, 517, 1097, 1560, 1836
SduI GDGCHC 5 cut(s) 568, 742, 968, 1451, 2047
SfaNI GCATC 3 cut(s) 628, 1327, 1696
SfcI CTRYAG 2 cut(s) 672, 1215
SfuI TTCGAA 1 cut(s) 1004
SinI GGWCC 2 cut(s) 983, 1511
SmiMI CAYNNNNRTG 4 cut(s) 173, 498, 1250, 2280
SmlI CTYRAG 3 cut(s) 10, 664, 1538
SmoI CTYRAG 3 cut(s) 10, 664, 1538
SpeI ACTAGT 1 cut(s) 1840
SphI GCATGC 1 cut(s) 2231
SseBI AGGCCT 1 cut(s) 474
SsiI CCGC 3 cut(s) 735, 1554, 1604
SspI AATATT 1 cut(s) 382
SspMI CTAG 7 cut(s) 336, 633, 1506, 1574, 1724, 1841, 1995
StuI AGGCCT 1 cut(s) 474
StyD4I CCNGG 5 cut(s) 286, 515, 1095, 1558, 1834
StyI CCWWGG 3 cut(s) 712, 1044, 1485
TaiI ACGT 1 cut(s) 1971
TaqI TCGA 5 cut(s) 1004, 1051, 1399, 1764, 1915
TatI WGTACW 1 cut(s) 91
TauI GCSGC 1 cut(s) 1556
TfiI GAWTC 8 cut(s) 146, 300, 455, 512, 1193, 1327, 1813, 2239
Tru1I TTAA 3 cut(s) 137, 1206, 1971
Tru9I TTAA 3 cut(s) 137, 1206, 1971
TscAI CASTG 4 cut(s) 175, 676, 867, 2191
TseFI GTSAC 2 cut(s) 1654, 1984
Tsp45I GTSAC 2 cut(s) 1654, 1984
TspDTI ATGAA 7 cut(s) 308, 480, 923, 1371, 1587, 1776, 2195
TspGWI ACGGA 2 cut(s) 1972, 2235
TspRI CASTG 4 cut(s) 175, 676, 867, 2191
VpaK11BI GGWCC 2 cut(s) 983, 1511
XapI RAATTY 4 cut(s) 620, 1055, 1401, 1783
XceI RCATGY 2 cut(s) 1941, 2231
XcmI CCANNNNNNNNNTGG 1 cut(s) 397
XmiI GTMKAC 4 cut(s) 210, 1152, 1421, 1845
XmnI GAANNNNTTC 1 cut(s) 1362
XspI CTAG 7 cut(s) 336, 633, 1506, 1574, 1724, 1841, 1995
Zsp2I ATGCAT 2 cut(s) 1373, 1689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.