Rh7DG090700

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0001127
Physical Location & Seq
Forward (+)
7613604 .. 7617098
3495 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG090700.1

Sequence Viewer

Length: 801 bp
ATGGGATCTCATCTAATGAAGAAGAGCAACAAAGTTCCACTGTTTGTGACATTCAATCTTTTCTTAGTCCTCTGTAGCCAAACCATCATAATTAGGACGGTGGACGACAAGAGTAAACTAAATTTACAGAATCTCAAGCTAAGAAGATGGCAGGGTCAAGCCCTGTTTGCAGGAACGGAAGGTGACTTTACTGGTTTGGTGTACCCGGAGAACCCAGGACTTATTCCTTCACTAGCCGGCGTCTGTGAGGCTCTTATACTCAATAACACACCTGTGGCTGGAAATGTAGTCCTTTGCTTTACATCAGTGGCCAGAAGAACTCCGGTAGCACTTGCTGTATCTAGTGTCAGAGCAGCTGGTGGGGTTGGAGTAATTGTTGCCAAGAGTCCTGGTGATGTCTTGGGTCCATGTAGCAGTGACTTTCCATGCATTGAAGTAGACTACGAGCTTGGTACTCAGATACTATTCTACATTCGCTCCACCAGTTCTCCTGTAGTAAAATTGAGTCCTTCTATGACACTGGTGGGGAAGCCTGTATCGACCAAGGTTGCCGCTTTCTCATCTAGAGGACCTAACTCCATTTCTCCAGCCATACTAAAGCCGGACATAGCTGCACCTGGTGTGAGCATATTAGCTGCCAGTTCTCCTTTTGATCCGTATATGAATGGAGGATTTGCCCTGCATTCAGGAACATCAATGGCAACTCCTCATGTTTCGGGGATTGTGGCACTTCTAAAAGCATTGCACTCCGATTGGTCCCCTGCTGCCATAAGATCAGCAATAACAACAACAGGTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

266

Amino Acids

27.75

Weight (kDa)

9.22

Isoelectric Point (pI)

45.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 111 - 264 2.1e-20 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 438
AciI CCGC 1 cut(s) 552
AclWI GGATC 2 cut(s) 13, 647
AcoI YGGCCR 1 cut(s) 309
AcsI RAATTY 1 cut(s) 121
AcyI GRCGYC 1 cut(s) 240
AdeI CACNNNGTG 1 cut(s) 620
AfaI GTAC 2 cut(s) 203, 454
AfiI CCNNNNNNNGG 1 cut(s) 278
AgsI TTSAA 2 cut(s) 55, 434
AjnI CCWGG 3 cut(s) 214, 388, 616
AleI CACNNNNGTG 1 cut(s) 272
AluBI AGCT 5 cut(s) 139, 356, 448, 611, 635
AluI AGCT 5 cut(s) 139, 356, 448, 611, 635
AlwI GGATC 2 cut(s) 13, 647
AoxI GGCC 1 cut(s) 309
ApeKI GCWGC 4 cut(s) 353, 611, 635, 764
ApoI RAATTY 1 cut(s) 121
AspS9I GGNCC 3 cut(s) 404, 569, 756
AsuC2I CCSGG 1 cut(s) 206
AsuHPI GGTGA 2 cut(s) 194, 404
AvaII GGWCC 3 cut(s) 404, 569, 756
BalI TGGCCA 1 cut(s) 311
BbvI GCAGC 4 cut(s) 365, 598, 622, 751
BccI CCATC 2 cut(s) 92, 141
BciT130I CCWGG 3 cut(s) 216, 390, 618
BcnI CCSGG 1 cut(s) 206
BfaI CTAG 3 cut(s) 233, 342, 564
BfmI CTRYAG 2 cut(s) 73, 492
BisI GCNGC 5 cut(s) 354, 552, 612, 636, 765
BlsI GCNGC 5 cut(s) 355, 553, 613, 637, 766
Bme1390I CCNGG 4 cut(s) 206, 216, 390, 618
Bme18I GGWCC 3 cut(s) 404, 569, 756
BmgT120I GGNCC 3 cut(s) 404, 569, 756
BmiI GGNNCC 2 cut(s) 405, 758
BmrFI CCNGG 4 cut(s) 206, 216, 390, 618
BpmI CTGGAG 1 cut(s) 570
BpuEI CTTGAG 1 cut(s) 119
BpuMI CCSGG 1 cut(s) 206
BsaHI GRCGYC 1 cut(s) 240
BsaJI CCNNGG 2 cut(s) 214, 543
BsaWI WCCGGW 1 cut(s) 322
BsaXI ACNNNNNCTCC 6 cut(s) 360, 390, 461, 472, 491, 502
Bsc4I CCNNNNNNNGG 1 cut(s) 278
Bse118I RCCGGY 1 cut(s) 236
Bse1I ACTGG 4 cut(s) 196, 483, 525, 639
Bse3DI GCAATG 1 cut(s) 740
BseBI CCWGG 3 cut(s) 216, 390, 618
BseDI CCNNGG 2 cut(s) 214, 543
BseLI CCNNNNNNNGG 1 cut(s) 278
BseMI GCAATG 1 cut(s) 740
BseMII CTCAG 1 cut(s) 470
BseNI ACTGG 4 cut(s) 196, 483, 525, 639
BseRI GAGGAG 1 cut(s) 696
BseXI GCAGC 4 cut(s) 365, 598, 622, 751
BsgI GTGCAG 1 cut(s) 597
BshFI GGCC 1 cut(s) 311
BsiSI CCGG 4 cut(s) 206, 237, 323, 602
BslFI GGGAC 1 cut(s) 742
BslI CCNNNNNNNGG 1 cut(s) 278
BsmFI GGGAC 1 cut(s) 742
BsmI GAATGC 1 cut(s) 682
BsnI GGCC 1 cut(s) 311
Bsp143I GATC 3 cut(s) 5, 652, 773
BspACI CCGC 1 cut(s) 552
BspANI GGCC 1 cut(s) 311
BspCNI CTCAG 1 cut(s) 469
BspLI GGNNCC 2 cut(s) 405, 758
BspPI GGATC 2 cut(s) 13, 647
BspQI GCTCTTC 1 cut(s) 17
BsrDI GCAATG 1 cut(s) 740
BsrFI RCCGGY 1 cut(s) 236
BsrI ACTGG 4 cut(s) 196, 483, 525, 639
BssAI RCCGGY 1 cut(s) 236
BssECI CCNNGG 2 cut(s) 214, 543
BssMI GATC 3 cut(s) 5, 652, 773
BssNI GRCGYC 1 cut(s) 240
BssT1I CCWWGG 1 cut(s) 543
Bst2UI CCWGG 3 cut(s) 216, 390, 618
Bst4CI ACNGT 2 cut(s) 42, 100
Bst6I CTCTTC 1 cut(s) 17
BstACI GRCGYC 1 cut(s) 240
BstC8I GCNNGC 1 cut(s) 238
BstDEI CTNAG 3 cut(s) 64, 140, 456
BstKTI GATC 3 cut(s) 8, 655, 776
BstMBI GATC 3 cut(s) 5, 652, 773
BstMWI GCNNNNNNNGC 1 cut(s) 167
BstNI CCWGG 3 cut(s) 216, 390, 618
BstSCI CCNGG 4 cut(s) 204, 214, 388, 616
BstSFI CTRYAG 2 cut(s) 73, 492
BstV1I GCAGC 4 cut(s) 365, 598, 622, 751
BstX2I RGATCY 1 cut(s) 5
BstYI RGATCY 1 cut(s) 5
BsuRI GGCC 1 cut(s) 311
BtsI GCAGTG 1 cut(s) 421
BtsIMutI CAGTG 4 cut(s) 38, 312, 421, 518
Cac8I GCNNGC 1 cut(s) 238
Cfr10I RCCGGY 1 cut(s) 236
Cfr13I GGNCC 3 cut(s) 404, 569, 756
CseI GACGC 1 cut(s) 229
CsiI ACCWGGT 1 cut(s) 616
Csp6I GTAC 2 cut(s) 202, 453
CviAII CATG 3 cut(s) 408, 426, 710
CviQI GTAC 2 cut(s) 202, 453
DdeI CTNAG 3 cut(s) 64, 140, 456
DpnI GATC 3 cut(s) 7, 654, 775
DpnII GATC 3 cut(s) 5, 652, 773
DraIII CACNNNGTG 1 cut(s) 620
EaeI YGGCCR 1 cut(s) 309
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
Eco130I CCWWGG 1 cut(s) 543
Eco47I GGWCC 3 cut(s) 404, 569, 756
EcoO109I RGGNCCY 1 cut(s) 569
EcoRII CCWGG 3 cut(s) 214, 388, 616
EcoT14I CCWWGG 1 cut(s) 543
EcoT22I ATGCAT 1 cut(s) 431
ErhI CCWWGG 1 cut(s) 543
FaeI CATG 3 cut(s) 411, 429, 713
FaqI GGGAC 1 cut(s) 742
FatI CATG 3 cut(s) 407, 425, 709
FblI GTMKAC 1 cut(s) 438
Fnu4HI GCNGC 5 cut(s) 354, 552, 612, 636, 765
Fsp4HI GCNGC 5 cut(s) 354, 552, 612, 636, 765
FspBI CTAG 3 cut(s) 233, 342, 564
GluI GCNGC 5 cut(s) 354, 552, 612, 636, 765
GsuI CTGGAG 1 cut(s) 570
HaeIII GGCC 1 cut(s) 311
HapII CCGG 4 cut(s) 206, 237, 323, 602
HgaI GACGC 1 cut(s) 229
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 3 cut(s) 411, 429, 713
HinfI GANTC 3 cut(s) 130, 385, 505
HpaII CCGG 4 cut(s) 206, 237, 323, 602
HphI GGTGA 2 cut(s) 194, 404
Hpy166II GTNNAC 4 cut(s) 103, 116, 202, 439
Hpy188I TCNGA 3 cut(s) 350, 459, 751
Hpy188III TCNNGA 2 cut(s) 564, 687
Hpy8I GTNNAC 4 cut(s) 103, 116, 202, 439
HpyAV CCTTC 3 cut(s) 173, 237, 519
HpyCH4III ACNGT 2 cut(s) 42, 100
HpyCH4V TGCA 5 cut(s) 170, 429, 614, 682, 745
HpyF10VI GCNNNNNNNGC 1 cut(s) 167
HpyF3I CTNAG 3 cut(s) 64, 140, 456
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 3 cut(s) 411, 429, 713
KroI GCCGGC 1 cut(s) 236
KroNI GCCGGC 1 cut(s) 238
Kzo9I GATC 3 cut(s) 5, 652, 773
LguI GCTCTTC 1 cut(s) 17
LmnI GCTCC 1 cut(s) 482
Lsp1109I GCAGC 4 cut(s) 365, 598, 622, 751
MabI ACCWGGT 1 cut(s) 616
MaeI CTAG 3 cut(s) 233, 342, 564
MaeIII GTNAC 3 cut(s) 46, 182, 416
MalI GATC 3 cut(s) 7, 654, 775
MboI GATC 3 cut(s) 5, 652, 773
MboII GAAGA 4 cut(s) 31, 34, 156, 327
MflI RGATCY 1 cut(s) 5
MlsI TGGCCA 1 cut(s) 311
MluCI AATT 4 cut(s) 90, 121, 372, 500
MluNI TGGCCA 1 cut(s) 311
MlyI GAGTC 2 cut(s) 394, 514
MmeI TCCRAC 1 cut(s) 346
MnlI CCTC 5 cut(s) 80, 241, 560, 662, 717
Mox20I TGGCCA 1 cut(s) 311
Mph1103I ATGCAT 1 cut(s) 431
MroNI GCCGGC 1 cut(s) 236
MscI TGGCCA 1 cut(s) 311
MseI TTAA 1 cut(s) 799
MslI CAYNNNNRTG 1 cut(s) 272
Msp20I TGGCCA 1 cut(s) 311
MspA1I CMGCKG 1 cut(s) 356
MspI CCGG 4 cut(s) 206, 237, 323, 602
MspR9I CCNGG 4 cut(s) 206, 216, 390, 618
Mva1269I GAATGC 1 cut(s) 682
MvaI CCWGG 3 cut(s) 216, 390, 618
MwoI GCNNNNNNNGC 1 cut(s) 167
NaeI GCCGGC 1 cut(s) 238
NciI CCSGG 1 cut(s) 206
NdeII GATC 3 cut(s) 5, 652, 773
NgoMIV GCCGGC 1 cut(s) 236
NlaIII CATG 3 cut(s) 411, 429, 713
NlaIV GGNNCC 2 cut(s) 405, 758
NmuCI GTSAC 3 cut(s) 46, 182, 416
NsiI ATGCAT 1 cut(s) 431
OliI CACNNNNGTG 1 cut(s) 272
PciSI GCTCTTC 1 cut(s) 17
PctI GAATGC 1 cut(s) 682
PdiI GCCGGC 1 cut(s) 238
PfeI GAWTC 1 cut(s) 130
PkrI GCNGC 5 cut(s) 355, 553, 613, 637, 766
PleI GAGTC 2 cut(s) 393, 513
PpsI GAGTC 2 cut(s) 393, 513
PpuMI RGGWCCY 1 cut(s) 569
Psp5II RGGWCCY 1 cut(s) 569
Psp6I CCWGG 3 cut(s) 214, 388, 616
PspGI CCWGG 3 cut(s) 214, 388, 616
PspN4I GGNNCC 2 cut(s) 405, 758
PspPI GGNCC 3 cut(s) 404, 569, 756
PspPPI RGGWCCY 1 cut(s) 569
PsuI RGATCY 1 cut(s) 5
PvuII CAGCTG 1 cut(s) 356
RsaI GTAC 2 cut(s) 203, 454
RsaNI GTAC 2 cut(s) 202, 453
RseI CAYNNNNRTG 1 cut(s) 272
SapI GCTCTTC 1 cut(s) 17
SaqAI TTAA 1 cut(s) 799
SatI GCNGC 5 cut(s) 354, 552, 612, 636, 765
Sau3AI GATC 3 cut(s) 5, 652, 773
Sau96I GGNCC 3 cut(s) 404, 569, 756
SchI GAGTC 2 cut(s) 394, 514
ScrFI CCNGG 4 cut(s) 206, 216, 390, 618
SexAI ACCWGGT 1 cut(s) 616
SfcI CTRYAG 2 cut(s) 73, 492
SinI GGWCC 3 cut(s) 404, 569, 756
SmiMI CAYNNNNRTG 1 cut(s) 272
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
Sse9I AATT 4 cut(s) 90, 121, 372, 500
SsiI CCGC 1 cut(s) 552
SspMI CTAG 3 cut(s) 233, 342, 564
StyD4I CCNGG 4 cut(s) 204, 214, 388, 616
StyI CCWWGG 1 cut(s) 543
TaaI ACNGT 2 cut(s) 42, 100
TaqI TCGA 1 cut(s) 539
TasI AATT 4 cut(s) 90, 121, 372, 500
TauI GCSGC 1 cut(s) 554
TfiI GAWTC 1 cut(s) 130
Tru1I TTAA 1 cut(s) 799
Tru9I TTAA 1 cut(s) 799
TscAI CASTG 4 cut(s) 45, 312, 421, 525
TseFI GTSAC 3 cut(s) 46, 182, 416
TseI GCWGC 4 cut(s) 353, 611, 635, 764
Tsp45I GTSAC 3 cut(s) 46, 182, 416
TspDTI ATGAA 2 cut(s) 32, 677
TspGWI ACGGA 2 cut(s) 191, 645
TspRI CASTG 4 cut(s) 45, 312, 421, 525
VpaK11BI GGWCC 3 cut(s) 404, 569, 756
XapI RAATTY 1 cut(s) 121
XbaI TCTAGA 1 cut(s) 563
XmiI GTMKAC 1 cut(s) 438
XspI CTAG 3 cut(s) 233, 342, 564
Zsp2I ATGCAT 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.