Rorug06G0483200

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001127
Physical Location & Seq
Forward (+)
60378237 .. 60380425
2189 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0483200.1

Sequence Viewer

Length: 1137 bp
ATGGAGTCTCTTGGTGTTTTTGCTGAGGGAGATCAATGGGAGTGCTTCAGCAGATTGTTCTCTAACGAAGATCAAGAGCTAGATTTCACACCGCATTTTCTTGGTCATGAAGGTTCATTTCCTTTCCAACACAATGAGGGATTCAATTTTGAAACCCCAACCACCCTTTTCCCCAATCCTGAAGCTGGTGATGAAATGGGATTGAACAGGTTCAATCAGAGCTTCTTTCATTCTTTAGACTCTCTCAGCTCTAATATGCACTATCTTCCTCAAGAAAATAATTCTGGTTATAGTGCTAGCAGTGGCATTTTTACTGCCATTCTGAACCCGGAGAATTACTACTTTGGTGGTAACTCTTGCCATACCCCAATGAGTAATGACATCTCTACATCCGTTGATGTTTCTATGACTGGTAATACTGAGAGCAATTTTGGGTCATTCATCCCGGCATTTAGTGACATTGGGATGGAAGGAGCTGTCTGTAACACTGAATATTCCGGCAGTGGCAGAATGGGCTTTTCAGATGACAGTCAACCACCAAAGGAACTGCAACTCAAGAGGATGCAGGATGCGGCGGCAGAGAAATCCAACACTGAAGATGCATCTGAGAGTAATCCAAAGAAGAAACCCCGTTTGTCGAAAGATAAAACTAAGAAGAGCAATGCGAGGTCAAAGAAGGGCCAAAAGGCTAACTCAGATCTGAAGGTGAAAGATGAAGACGAGAGTAACACAGGAGTGGACAGACAGAGCTCTAGCTCTTATAACAGCTCTGAGGATGACAATGCTTCTCAAGAAACCAATGGAGCAGGAGAAATTTCAGACCCCAAATCATCATCAGCCCTTAACTTGAATGGCAAGACAAGAGCCAATAGAGGGTCTGCAACTGATCCCCAGAGCCTCTATGCTAGGAAACGAAGGGAGAGAATCAACGAGCGATTGAGAGTCTTGCAGAATCTTGTCCCTAATGGAACAAAGGTTGACATCAGCACAATGCTGGAAGAGGCAGTCCATTATGTCAAGTTTTTACAGCTCCAGATCAAGCTTTTGAGCTCCGATGACATGTGGATGTATGCTCCTATTGCTTATAACGGAATGGATATCGGTCTCGACCTGCATAAGATGTCTCCACTTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

378

Amino Acids

41.88

Weight (kDa)

4.95

Isoelectric Point (pI)

50.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 303 - 343 2.4e-08 Helix-loop-helix DNA-binding domain
bHLH_SAC51 PF23173 304 - 350 7.4e-06 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 762, 1086
Acc36I ACCTGC 1 cut(s) 1119
AciI CCGC 3 cut(s) 92, 572, 575
AclWI GGATC 1 cut(s) 881
AcsI RAATTY 1 cut(s) 813
AcuI CTGAAG 4 cut(s) 31, 201, 615, 722
AfiI CCNNNNNNNGG 2 cut(s) 185, 873
AflIII ACRYGT 1 cut(s) 1059
AgsI TTSAA 5 cut(s) 145, 152, 205, 214, 850
AleI CACNNNNGTG 1 cut(s) 734
Alw21I GWGCWC 2 cut(s) 752, 1052
Alw26I GTCTC 3 cut(s) 12, 1109, 1128
AlwI GGATC 1 cut(s) 881
AoxI GGCC 1 cut(s) 679
ApoI RAATTY 1 cut(s) 813
Asp700I GAANNNNTTC 1 cut(s) 209
AspS9I GGNCC 1 cut(s) 679
AsuC2I CCSGG 2 cut(s) 329, 446
AsuHPI GGTGA 2 cut(s) 200, 718
AsuNHI GCTAGC 1 cut(s) 296
BanII GRGCYC 2 cut(s) 752, 1052
BbsI GAAGAC 1 cut(s) 723
Bbv12I GWGCWC 2 cut(s) 752, 1052
BbvCI CCTCAGC 1 cut(s) 24
BccI CCATC 1 cut(s) 460
BcnI CCSGG 2 cut(s) 329, 446
BcoDI GTCTC 3 cut(s) 12, 1109, 1128
BfaI CTAG 4 cut(s) 80, 297, 753, 906
BfuAI ACCTGC 1 cut(s) 1119
BglII AGATCT 1 cut(s) 697
BisI GCNGC 2 cut(s) 573, 576
BlsI GCNGC 2 cut(s) 574, 577
Bme1390I CCNGG 2 cut(s) 329, 446
BmgT120I GGNCC 1 cut(s) 679
BmrFI CCNGG 2 cut(s) 329, 446
BmsI GCATC 4 cut(s) 552, 559, 589, 611
BmtI GCTAGC 1 cut(s) 300
BpiI GAAGAC 1 cut(s) 723
BpmI CTGGAG 1 cut(s) 1016
Bpu10I CCTNAGC 1 cut(s) 24
BpuEI CTTGAG 3 cut(s) 255, 539, 774
BpuMI CCSGG 2 cut(s) 329, 446
BsaI GGTCTC 1 cut(s) 1109
Bsc4I CCNNNNNNNGG 2 cut(s) 185, 873
Bse1I ACTGG 1 cut(s) 415
Bse3DI GCAATG 1 cut(s) 667
BseGI GGATG 7 cut(s) 389, 441, 471, 567, 574, 781, 1071
BseLI CCNNNNNNNGG 2 cut(s) 185, 873
BseMI GCAATG 1 cut(s) 667
BseMII CTCAG 6 cut(s) 15, 259, 411, 597, 708, 762
BseNI ACTGG 1 cut(s) 415
BshFI GGCC 1 cut(s) 681
BsiHKAI GWGCWC 2 cut(s) 752, 1052
BsiSI CCGG 3 cut(s) 329, 446, 498
BslFI GGGAC 1 cut(s) 944
BslI CCNNNNNNNGG 2 cut(s) 185, 873
BsmAI GTCTC 3 cut(s) 12, 1109, 1128
BsmFI GGGAC 1 cut(s) 944
BsnI GGCC 1 cut(s) 681
Bso31I GGTCTC 1 cut(s) 1109
Bsp1286I GDGCHC 2 cut(s) 752, 1052
Bsp143I GATC 5 cut(s) 31, 70, 697, 886, 1035
BspACI CCGC 3 cut(s) 92, 572, 575
BspANI GGCC 1 cut(s) 681
BspCNI CTCAG 6 cut(s) 16, 258, 412, 598, 707, 763
BspHI TCATGA 1 cut(s) 106
BspMI ACCTGC 1 cut(s) 1119
BspOI GCTAGC 1 cut(s) 300
BspPI GGATC 1 cut(s) 881
BspQI GCTCTTC 1 cut(s) 650
BspTNI GGTCTC 1 cut(s) 1109
BsrDI GCAATG 1 cut(s) 667
BsrI ACTGG 1 cut(s) 415
BssMI GATC 5 cut(s) 31, 70, 697, 886, 1035
Bst4CI ACNGT 1 cut(s) 530
Bst6I CTCTTC 2 cut(s) 650, 993
BstC8I GCNNGC 1 cut(s) 298
BstDEI CTNAG 7 cut(s) 24, 245, 420, 606, 651, 694, 771
BstF5I GGATG 7 cut(s) 389, 441, 471, 567, 574, 781, 1071
BstKTI GATC 5 cut(s) 34, 73, 700, 889, 1038
BstMAI GTCTC 3 cut(s) 12, 1109, 1128
BstMBI GATC 5 cut(s) 31, 70, 697, 886, 1035
BstMWI GCNNNNNNNGC 2 cut(s) 513, 1079
BstNSI RCATGY 1 cut(s) 1063
BstSCI CCNGG 2 cut(s) 327, 444
BstV2I GAAGAC 1 cut(s) 723
BstX2I RGATCY 1 cut(s) 697
BstYI RGATCY 1 cut(s) 697
BsuRI GGCC 1 cut(s) 681
BtsCI GGATG 7 cut(s) 389, 441, 471, 567, 574, 781, 1071
BtsI GCAGTG 2 cut(s) 307, 508
BtsIMutI CAGTG 4 cut(s) 307, 486, 508, 591
BveI ACCTGC 1 cut(s) 1119
Cac8I GCNNGC 1 cut(s) 298
CciI TCATGA 1 cut(s) 106
Cfr13I GGNCC 1 cut(s) 679
CviAII CATG 2 cut(s) 107, 1060
DdeI CTNAG 7 cut(s) 24, 245, 420, 606, 651, 694, 771
DpnI GATC 5 cut(s) 33, 72, 699, 888, 1037
DpnII GATC 5 cut(s) 31, 70, 697, 886, 1035
Eam1104I CTCTTC 2 cut(s) 650, 993
EarI CTCTTC 2 cut(s) 650, 993
Ecl136II GAGCTC 2 cut(s) 750, 1050
Eco24I GRGCYC 2 cut(s) 752, 1052
Eco31I GGTCTC 1 cut(s) 1109
Eco32I GATATC 1 cut(s) 1099
Eco53kI GAGCTC 2 cut(s) 750, 1050
Eco57I CTGAAG 4 cut(s) 31, 201, 615, 722
EcoICRI GAGCTC 2 cut(s) 750, 1050
EcoRV GATATC 1 cut(s) 1099
EcoT22I ATGCAT 1 cut(s) 604
EcoT38I GRGCYC 2 cut(s) 752, 1052
FaeI CATG 2 cut(s) 110, 1063
FaqI GGGAC 1 cut(s) 944
FatI CATG 2 cut(s) 106, 1059
Fnu4HI GCNGC 2 cut(s) 573, 576
FokI GGATG 7 cut(s) 376, 428, 478, 574, 581, 788, 1078
FriOI GRGCYC 2 cut(s) 752, 1052
Fsp4HI GCNGC 2 cut(s) 573, 576
FspBI CTAG 4 cut(s) 80, 297, 753, 906
GluI GCNGC 2 cut(s) 573, 576
GsuI CTGGAG 1 cut(s) 1016
HaeIII GGCC 1 cut(s) 681
HapII CCGG 3 cut(s) 329, 446, 498
Hin1II CATG 2 cut(s) 110, 1063
HincII GTYRAC 2 cut(s) 533, 979
HindII GTYRAC 2 cut(s) 533, 979
HindIII AAGCTT 1 cut(s) 1040
HinfI GANTC 6 cut(s) 5, 141, 239, 924, 942, 952
HpaII CCGG 3 cut(s) 329, 446, 498
HphI GGTGA 2 cut(s) 200, 718
Hpy166II GTNNAC 3 cut(s) 533, 739, 979
Hpy188I TCNGA 9 cut(s) 219, 324, 523, 607, 697, 702, 772, 820, 1054
Hpy188III TCNNGA 8 cut(s) 74, 107, 179, 272, 556, 791, 1033, 1106
Hpy8I GTNNAC 3 cut(s) 533, 739, 979
HpyAV CCTTC 5 cut(s) 104, 464, 670, 697, 909
HpyCH4III ACNGT 1 cut(s) 530
HpyCH4V TGCA 7 cut(s) 259, 550, 565, 602, 881, 949, 1114
HpyF10VI GCNNNNNNNGC 2 cut(s) 513, 1079
HpyF3I CTNAG 7 cut(s) 24, 245, 420, 606, 651, 694, 771
Hsp92II CATG 2 cut(s) 110, 1063
Kzo9I GATC 5 cut(s) 31, 70, 697, 886, 1035
LguI GCTCTTC 1 cut(s) 650
LmnI GCTCC 5 cut(s) 473, 803, 1035, 1055, 1078
LweI GCATC 4 cut(s) 552, 559, 589, 611
MaeI CTAG 4 cut(s) 80, 297, 753, 906
MaeIII GTNAC 4 cut(s) 350, 455, 482, 725
MalI GATC 5 cut(s) 33, 72, 699, 888, 1037
MboI GATC 5 cut(s) 31, 70, 697, 886, 1035
MboII GAAGA 7 cut(s) 80, 257, 608, 634, 667, 728, 1010
MflI RGATCY 1 cut(s) 697
MhlI GDGCHC 2 cut(s) 752, 1052
MluCI AATT 5 cut(s) 145, 280, 334, 427, 813
MlyI GAGTC 3 cut(s) 14, 233, 951
MmeI TCCRAC 2 cut(s) 151, 612
MnlI CCTC 9 cut(s) 19, 130, 279, 552, 660, 766, 866, 908, 994
Mph1103I ATGCAT 1 cut(s) 604
MroXI GAANNNNTTC 1 cut(s) 209
MseI TTAA 1 cut(s) 843
MslI CAYNNNNRTG 4 cut(s) 464, 734, 1064, 1132
MspI CCGG 3 cut(s) 329, 446, 498
MspR9I CCNGG 2 cut(s) 329, 446
MwoI GCNNNNNNNGC 2 cut(s) 513, 1079
NciI CCSGG 2 cut(s) 329, 446
NdeII GATC 5 cut(s) 31, 70, 697, 886, 1035
NheI GCTAGC 1 cut(s) 296
NlaIII CATG 2 cut(s) 110, 1063
NmuCI GTSAC 1 cut(s) 455
NsiI ATGCAT 1 cut(s) 604
NspI RCATGY 1 cut(s) 1063
OliI CACNNNNGTG 1 cut(s) 734
PagI TCATGA 1 cut(s) 106
PciI ACATGT 1 cut(s) 1059
PciSI GCTCTTC 1 cut(s) 650
PdmI GAANNNNTTC 1 cut(s) 209
PfeI GAWTC 3 cut(s) 141, 924, 952
PkrI GCNGC 2 cut(s) 574, 577
PleI GAGTC 3 cut(s) 13, 233, 950
PpsI GAGTC 3 cut(s) 13, 233, 950
PscI ACATGT 1 cut(s) 1059
PsiI TTATAA 2 cut(s) 762, 1086
Psp124BI GAGCTC 2 cut(s) 752, 1052
PspPI GGNCC 1 cut(s) 679
PsuI RGATCY 1 cut(s) 697
RseI CAYNNNNRTG 4 cut(s) 464, 734, 1064, 1132
SacI GAGCTC 2 cut(s) 752, 1052
SapI GCTCTTC 1 cut(s) 650
SaqAI TTAA 1 cut(s) 843
SatI GCNGC 2 cut(s) 573, 576
Sau3AI GATC 5 cut(s) 31, 70, 697, 886, 1035
Sau96I GGNCC 1 cut(s) 679
SchI GAGTC 3 cut(s) 14, 233, 951
ScrFI CCNGG 2 cut(s) 329, 446
SduI GDGCHC 2 cut(s) 752, 1052
SfaNI GCATC 4 cut(s) 552, 559, 589, 611
SmiMI CAYNNNNRTG 4 cut(s) 464, 734, 1064, 1132
SmlI CTYRAG 3 cut(s) 270, 554, 789
SmoI CTYRAG 3 cut(s) 270, 554, 789
Sse9I AATT 5 cut(s) 145, 280, 334, 427, 813
SsiI CCGC 3 cut(s) 92, 572, 575
SspI AATATT 1 cut(s) 494
SspMI CTAG 4 cut(s) 80, 297, 753, 906
SstI GAGCTC 2 cut(s) 752, 1052
StyD4I CCNGG 2 cut(s) 327, 444
TaaI ACNGT 1 cut(s) 530
TaqI TCGA 2 cut(s) 638, 1107
TaqII GACCGA 1 cut(s) 1091
TasI AATT 5 cut(s) 145, 280, 334, 427, 813
TauI GCSGC 2 cut(s) 575, 578
TfiI GAWTC 3 cut(s) 141, 924, 952
Tru1I TTAA 1 cut(s) 843
Tru9I TTAA 1 cut(s) 843
TscAI CASTG 4 cut(s) 307, 493, 508, 598
TseFI GTSAC 1 cut(s) 455
Tsp45I GTSAC 1 cut(s) 455
TspDTI ATGAA 6 cut(s) 105, 123, 207, 218, 430, 729
TspGWI ACGGA 2 cut(s) 382, 1104
TspRI CASTG 4 cut(s) 307, 493, 508, 598
XapI RAATTY 1 cut(s) 813
XceI RCATGY 1 cut(s) 1063
XmnI GAANNNNTTC 1 cut(s) 209
XspI CTAG 4 cut(s) 80, 297, 753, 906
Zsp2I ATGCAT 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.