Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0001127

Physical Location & Seq

Genomic Coordinates Forward (+)

11941048 .. 11944824

Gene Structure 3777 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G32950.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2322 bp

ATGAGGAATTTTAGATCATCAGTTCTTGTGGTGCTAAGTCTGATAATAGTTTTGAATGTCGCACGAGCTAGTGCTAAGAGCAAGGTTCATATAGTGTATCTGGGGGAGAAGCAACATGATGATCCTAAATTTGTCACAGAATCTCATCACCAGATGTTGTCTTCACTTCTTGGAAGTAAAGATGATGCCCATGAGTCAATGGTGTATAGTTATCGACATGGATTCTCAGGCTTTGCGGCCAAACTCACCAAGTCACAAGCCAAGAAAATCGCTGATTCTCCTGAAGTTATCCATGTCATACCGGATAGTTACTATGAACTTGCAACAACGCGGATTTGGGACTACTTAGGTCCTTCTGCTGATAATTCGAAGAATCTCGTAAGTGATACAAACATGGGTGATCAAACTATCATTGGTGTTATTGACACAGGAGTGTGGCCAGAGTCTGAATCATTTAACGACTATGGGGTTGGACCAGTACCGAGCCATTGGAAAGGAGGTTGTGAACCCGGTGAAAACTTCATATCAACAAATTGCAACAGGAAGTTGATAGGAGCCAAGTACTTTATCAACGGTTTTCTTGCTGAAAACCAATTCAACGCCACAGAATCCCCTGACTATATATCCGCTAGAGACTTTGACGGCCATGGCACGCATGTCGCCTCCATTGCGGGTGGTTCATTTGTTCCTAATGTAAGCTACAAAGGCCTTGGTAGAGGAACCTTGAGAGGTGGTGCACCTCGTGCTCGCATAGCAATGTACAAGGCTTGTTGGTATATAAACGAGCTAGACGGAGTGACATGTTCATTTTCTGACATCATGAAAGCAATTGACGAAGCTATTCATGATGGTGTTGACGTTTTGTCAATCTCTCTCGGTGGTCGAGTTCCTCTGAATTCTGAAACCGATCTACGTGATGGGATTGCTACCGGAGCATTCCATGCAGTCGCAAAGGGCATTGTAGTTGTTTGTGCTGGTGGTAATGCTGGCCCATCATCTCAGACTGTGGTAAACACGGCTCCTTGGATCTTAACAGTGGCTGCAACCACTCTAGACCGGTCCTTTGCCACACCTATTATACTTGGGAACAATCAAGTGATATTGGGTCAAGCAATGTACATAGGTCCGGAGCTTGGCTTCACTAGTTTGGTTTACCCAGAGGATCCAGGGAACAGTATTGACACCTTTAGCGGTGTCTGTGAGTCCCTTAATCTCAACTCTAACCGTACAATGGCCGGGAAAGTTGTATTGTGTTTCACAACAGCAAGAGATTTCACTGTTGTATCCACAGCTGCGTCTATTGTGAAGGCAGCCGGTGGTCTTGGCCTGATCATTGCAAGAAACCCGGGGTATAATCTCGCTCCATGTAGTGATGATTTTCCTTGTGTAGCCATTGACAATGAGCTTGGGACCGATATACTTTTCTACATACGTTATACTGGATCACCTGTTGTGAAGATACAACCTTCTAGAACACTCGTCGGAGAACCTGTTGGGACAAAGGTAGCAACTTTCTCATCAAGAGGGCCTAACTCGATTTCCCCAGCGATTCTGAAACCTGATATAGCAGCACCCGGTGTGAGCATTTTAGCTGCTACATCTCCCAATGACACCCTCAATGCCGGTGGATTTGTTATGCGTTCAGGAACATCAATGGCGGCTCCAGTTATTTCAGGAGTTATTGCACTTCTCAAGTCTTTGCATCCTGATTGGTCTCCTGCTGCTTTTAGATCAGCCATTGTCACTACAGCTTGGAGAACAGATCCATTCGGAGAGCAGATAGCTGCGGAAAGTTCATCCCTCAAAGTACCTGATCCGTTTGATTATGGTGGAGGCCTCGTGAACCCAGAGAAAGCTGCAGAACCAGGTCTCATACTCGACATGGACTCACAAGACTATGTTCTCTACCTGTGTTCCGCTGGTTACAACGACTCATCAATCTCTCGGCTTGTCGGTAAAGTAACAGTCTGTTCAAACCCCAAACCTTCTGTCCTGGATATCAACTTACCTTCAATCACCATCCCAAACCTCAAAGACGAAGTCACTCTCACCAGAACCGTCACTAACGTTGGACCAGTCGACTCGGTCTACAAGGTCTTGGTTGAGCCTCCGCTTGGGATTCAAGTGGTTGTTACACCAGAGACACTAGTGTTTAACTCCAAAACCAAAAGTGTCTCCTTCACGGTTATTGTCTCGACCACACACAAAATAAACACTGGATTCTACTTTGGTAGCTTGACTTGGACTGATTCTATCCATAATGTGGTCATTCCTGTTTCCGTGAGAACGCAGATTCTGCAAAACTATTACGATGAGAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009505 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009682 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0016043 GO:0016049 GO:0016787 GO:0017171 GO:0019538 GO:0022414 GO:0030154 GO:0030312 GO:0031012 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0042742 GO:0043170 GO:0043207 GO:0044238 GO:0044421 GO:0044464 GO:0044706 GO:0045087 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0050896 GO:0051704 GO:0051707 GO:0060560 GO:0070011 GO:0071704 GO:0071840 GO:0071944 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

773

Amino Acids

82.44

Weight (kDa)

5.57

Isoelectric Point (pI)

29.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	2 - 56	8.4e-16	Peptidase inhibitor I9
Peptidase_S8	PF00082	82 - 536	3.3e-45	Subtilase family
fn3_6	PF17766	615 - 710	4.5e-27	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001127)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G32940 AT1G32940 AT1G32940 AT1G32940 AT1G32950 AT1G32950 AT1G32960 AT4G10510 AT4G10510 AT4G10510 AT4G10510 AT4G10520 AT4G10530 AT4G10540 AT4G10550 AT4G10550 AT4G10550 AT4G10550
fragaria_vesca	FvH4_5g10490 FvH4_5g10502
malus_domestica	MD00G1035000.v1.1
prunus_persica	Prupe.5G133500_v2.0.a1 Prupe.5G133500_v2.0.a1 Prupe.5G133500_v2.0.a1 Prupe.5G133500_v2.0.a1 Prupe.5G133500_v2.0.a1 Prupe.5G133600_v2.0.a1 Prupe.5G133700_v2.0.a1
pyrus_communis	pycom01g12030 pycom14g11430 pycom14g11440 pycom14g11450 pycom14g11470 pycom14g11480 pycom14g11520
rosa_chinensis	RchiOBHm_Chr4g0389671 RchiOBHm_Chr7g0187621 RchiOBHm_Chr7g0187651
rosa_laevigata	RLG00000004761 RLG00000004765
rosa_multiflora	Rmu_sc0003144.1_g000021 Rmu_sc0004483.1_g000012 Rmu_ssc0000110.1_g000014
rosa_roxburghii	Rroxscaffold_3G00267240
rosa_rugosa	Rorug06G0483200
rosa_samantha	Rh4AG029600 Rh4BG022700 Rh7AG088100 Rh7AG088500 Rh7BG089400 Rh7BG089900 Rh7BG090200 Rh7CG088700 Rh7CG089100 Rh7DG090400 Rh7DG090700
rosa_wichuraiana	Rw0G002650 Rw4G002200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	2263
AccI	GTMKAC	2 cut(s)	2079, 2088
AccII	CGCG	1 cut(s)	331
AccIII	TCCGGA	1 cut(s)	1126
AciI	CCGC	9 cut(s)	236, 331, 627, 671, 1191, 1658, 1787, 1917, 2111
AclI	AACGTT	1 cut(s)	2067
AclWI	GGATC	7 cut(s)	116, 1034, 1157, 1170, 1450, 1757, 1808
AcoI	YGGCCR	4 cut(s)	237, 437, 643, 1233
AcsI	RAATTY	3 cut(s)	7, 128, 895
AcuI	CTGAAG	1 cut(s)	303
AdeI	CACNNNGTG	2 cut(s)	743, 1577
AfaI	GTAC	6 cut(s)	480, 563, 761, 1118, 1228, 1809
AfiI	CCNNNNNNNGG	4 cut(s)	1133, 1231, 2114, 2263
AflIII	ACRYGT	1 cut(s)	800
AgeI	ACCGGT	1 cut(s)	1056
AgsI	TTSAA	5 cut(s)	55, 598, 1974, 2013, 2123
AhdI	GACNNNNNGTC	1 cut(s)	862
AhlI	ACTAGT	2 cut(s)	1142, 2146
AjnI	CCWGG	3 cut(s)	1165, 1864, 1992
AleI	CACNNNNGTG	1 cut(s)	431
Alw21I	GWGCWC	2 cut(s)	739, 748
Alw26I	GTCTC	6 cut(s)	627, 1719, 1874, 2135, 2179, 2197
Alw44I	GTGCAC	1 cut(s)	735
AlwI	GGATC	7 cut(s)	116, 1034, 1157, 1170, 1450, 1757, 1808
AlwNI	CAGNNNCTG	3 cut(s)	446, 1040, 2296
Ama87I	CYCGRG	1 cut(s)	1345
Aor13HI	TCCGGA	1 cut(s)	1126
AoxI	GGCC	9 cut(s)	237, 437, 643, 706, 988, 1233, 1324, 1526, 1834
ApaLI	GTGCAC	1 cut(s)	735
ApeKI	GCWGC	8 cut(s)	1040, 1292, 1310, 1568, 1592, 1721, 1784, 1856
ApoI	RAATTY	3 cut(s)	7, 128, 895
ArsI	GACNNNNNNTTYG	6 cut(s)	1046, 1078, 1974, 2006, 2025, 2057
AsiGI	ACCGGT	1 cut(s)	1056
Asp700I	GAANNNNTTC	1 cut(s)	840
AspS9I	GGNCC	8 cut(s)	350, 473, 989, 1059, 1124, 1410, 1526, 2072
AsuC2I	CCSGG	5 cut(s)	510, 1237, 1346, 1347, 1575
AsuHPI	GGTGA	7 cut(s)	140, 238, 410, 524, 1437, 2008, 2041
AsuII	TTCGAA	1 cut(s)	368
AvaI	CYCGRG	1 cut(s)	1345
AvaII	GGWCC	6 cut(s)	350, 473, 1059, 1124, 1410, 2072
BaeGI	GKGCMC	1 cut(s)	739
BaeI	ACNNNNGTAYC	2 cut(s)	1266, 1299
BalI	TGGCCA	1 cut(s)	439
BamHI	GGATCC	1 cut(s)	1162
BauI	CACGAG	3 cut(s)	63, 741, 1838
BbsI	GAAGAC	1 cut(s)	153
Bbv12I	GWGCWC	2 cut(s)	739, 748
BbvI	GCAGC	8 cut(s)	1027, 1279, 1322, 1579, 1580, 1708, 1771, 1843
BccI	CCATC	4 cut(s)	842, 911, 1000, 2027
BceAI	ACGGC	2 cut(s)	658, 1032
BcgI	CGANNNNNNTGC	2 cut(s)	640, 674
BciT130I	CCWGG	3 cut(s)	1167, 1866, 1994
BciVI	GTATCC	1 cut(s)	1294
BclI	TGATCA	2 cut(s)	400, 1329
BcnI	CCSGG	5 cut(s)	510, 1237, 1346, 1347, 1575
BcoDI	GTCTC	6 cut(s)	627, 1719, 1874, 2135, 2179, 2197
BcuI	ACTAGT	2 cut(s)	1142, 2146
BfaI	CTAG	7 cut(s)	69, 630, 788, 1052, 1143, 1470, 2147
BfmI	CTRYAG	2 cut(s)	1746, 1857
BfuI	GTATCC	1 cut(s)	1294
BmcAI	AGTACT	1 cut(s)	563
Bme1390I	CCNGG	8 cut(s)	510, 1167, 1237, 1346, 1347, 1575, 1866, 1994
Bme18I	GGWCC	6 cut(s)	350, 473, 1059, 1124, 1410, 2072
BmeRI	GACNNNNNGTC	1 cut(s)	862
BmeT110I	CYCGRG	1 cut(s)	1345
BmgT120I	GGNCC	8 cut(s)	350, 473, 989, 1059, 1124, 1410, 1526, 2072
BmiI	GGNNCC	6 cut(s)	556, 721, 1020, 1164, 1411, 1662
BmrFI	CCNGG	8 cut(s)	510, 1167, 1237, 1346, 1347, 1575, 1866, 1994
BmsI	GCATC	2 cut(s)	175, 1711
BpiI	GAAGAC	1 cut(s)	153
BpmI	CTGGAG	1 cut(s)	1647
Bpu14I	TTCGAA	1 cut(s)	368
BpuEI	CTTGAG	2 cut(s)	745, 1676
BpuMI	CCSGG	5 cut(s)	510, 1237, 1346, 1347, 1575
BsaAI	YACGTR	1 cut(s)	914
BsaI	GGTCTC	2 cut(s)	1719, 1874
BsaJI	CCNNGG	6 cut(s)	646, 709, 1022, 1166, 1345, 1346
BsaWI	WCCGGW	4 cut(s)	301, 929, 1056, 1126
BsaXI	ACNNNNNCTCC	4 cut(s)	1476, 1506, 1824, 1854
Bsc4I	CCNNNNNNNGG	4 cut(s)	1133, 1231, 2114, 2263
Bse118I	RCCGGY	3 cut(s)	1056, 1313, 1622
Bse1I	ACTGG	5 cut(s)	476, 1444, 1664, 2075, 2221
Bse3DI	GCAATG	4 cut(s)	666, 762, 1119, 1332
BseAI	TCCGGA	1 cut(s)	1126
BseBI	CCWGG	3 cut(s)	1167, 1866, 1994
BseDI	CCNNGG	6 cut(s)	646, 709, 1022, 1166, 1345, 1346
BseGI	GGATG	3 cut(s)	1702, 1796, 2019
BseLI	CCNNNNNNNGG	4 cut(s)	1133, 1231, 2114, 2263
BseMI	GCAATG	4 cut(s)	666, 762, 1119, 1332
BseMII	CTCAG	2 cut(s)	240, 1013
BseNI	ACTGG	5 cut(s)	476, 1444, 1664, 2075, 2221
BseSI	GKGCMC	1 cut(s)	739
BseXI	GCAGC	8 cut(s)	1027, 1279, 1322, 1579, 1580, 1708, 1771, 1843
BseYI	CCCAGC	1 cut(s)	1543
Bsh1236I	CGCG	1 cut(s)	331
BshFI	GGCC	9 cut(s)	239, 439, 645, 708, 990, 1235, 1326, 1528, 1836
BshTI	ACCGGT	1 cut(s)	1056
BsiHKAI	GWGCWC	2 cut(s)	739, 748
BsiHKCI	CYCGRG	1 cut(s)	1345
BslFI	GGGAC	4 cut(s)	353, 1189, 1423, 1510
BslI	CCNNNNNNNGG	4 cut(s)	1133, 1231, 2114, 2263
BsmAI	GTCTC	6 cut(s)	627, 1719, 1874, 2135, 2179, 2197
BsmFI	GGGAC	4 cut(s)	353, 1189, 1423, 1510
BsmI	GAATGC	1 cut(s)	935
BsnI	GGCC	9 cut(s)	239, 439, 645, 708, 990, 1235, 1326, 1528, 1836
Bso31I	GGTCTC	2 cut(s)	1719, 1874
BsoBI	CYCGRG	1 cut(s)	1345
Bsp119I	TTCGAA	1 cut(s)	368
Bsp1286I	GDGCHC	2 cut(s)	739, 748
Bsp13I	TCCGGA	1 cut(s)	1126
Bsp1407I	TGTACA	2 cut(s)	759, 1116
Bsp19I	CCATGG	1 cut(s)	646
BspACI	CCGC	9 cut(s)	236, 331, 627, 671, 1191, 1658, 1787, 1917, 2111
BspANI	GGCC	9 cut(s)	239, 439, 645, 708, 990, 1235, 1326, 1528, 1836
BspCNI	CTCAG	2 cut(s)	239, 1012
BspEI	TCCGGA	1 cut(s)	1126
BspFNI	CGCG	1 cut(s)	331
BspHI	TCATGA	2 cut(s)	819, 844
BspLI	GGNNCC	6 cut(s)	556, 721, 1020, 1164, 1411, 1662
BspMAI	CTGCAG	1 cut(s)	1861
BspPI	GGATC	7 cut(s)	116, 1034, 1157, 1170, 1450, 1757, 1808
BspT104I	TTCGAA	1 cut(s)	368
BspTNI	GGTCTC	2 cut(s)	1719, 1874
BsrDI	GCAATG	4 cut(s)	666, 762, 1119, 1332
BsrFI	RCCGGY	3 cut(s)	1056, 1313, 1622
BsrGI	TGTACA	2 cut(s)	759, 1116
BsrI	ACTGG	5 cut(s)	476, 1444, 1664, 2075, 2221
BssAI	RCCGGY	3 cut(s)	1056, 1313, 1622
BssECI	CCNNGG	6 cut(s)	646, 709, 1022, 1166, 1345, 1346
BssSI	CACGAG	3 cut(s)	63, 741, 1838
BssT1I	CCWWGG	3 cut(s)	646, 709, 1022
Bst2BI	CACGAG	3 cut(s)	63, 741, 1838
Bst2UI	CCWGG	3 cut(s)	1167, 1866, 1994
Bst4CI	ACNGT	9 cut(s)	575, 1006, 1036, 1175, 1226, 1279, 1966, 2059, 2185
BstAPI	GCANNNNNTGC	3 cut(s)	743, 941, 2296
BstAUI	TGTACA	2 cut(s)	759, 1116
BstBAI	YACGTR	1 cut(s)	914
BstBI	TTCGAA	1 cut(s)	368
BstC8I	GCNNGC	3 cut(s)	653, 748, 988
BstDEI	CTNAG	5 cut(s)	35, 75, 226, 346, 999
BstDSI	CCRYGG	1 cut(s)	646
BstF5I	GGATG	3 cut(s)	1702, 1796, 2019
BstFNI	CGCG	1 cut(s)	331
BstMAI	GTCTC	6 cut(s)	627, 1719, 1874, 2135, 2179, 2197
BstMWI	GCNNNNNNNGC	7 cut(s)	668, 705, 743, 752, 932, 941, 2296
BstNI	CCWGG	3 cut(s)	1167, 1866, 1994
BstNSI	RCATGY	2 cut(s)	659, 804
BstSCI	CCNGG	8 cut(s)	508, 1165, 1235, 1344, 1345, 1573, 1864, 1992
BstSFI	CTRYAG	2 cut(s)	1746, 1857
BstSLI	GKGCMC	1 cut(s)	739
BstUI	CGCG	1 cut(s)	331
BstV1I	GCAGC	8 cut(s)	1027, 1279, 1322, 1579, 1580, 1708, 1771, 1843
BstV2I	GAAGAC	1 cut(s)	153
BstX2I	RGATCY	3 cut(s)	1026, 1162, 1762
BstYI	RGATCY	3 cut(s)	1026, 1162, 1762
BsuI	GTATCC	1 cut(s)	1294
BsuRI	GGCC	9 cut(s)	239, 439, 645, 708, 990, 1235, 1326, 1528, 1836
BtgI	CCRYGG	1 cut(s)	646
BtsCI	GGATG	3 cut(s)	1702, 1796, 2019
BtsIMutI	CAGTG	3 cut(s)	1041, 1275, 2214
Cac8I	GCNNGC	3 cut(s)	653, 748, 988
CaiI	CAGNNNCTG	3 cut(s)	446, 1040, 2296
CciI	TCATGA	2 cut(s)	819, 844
Cfr10I	RCCGGY	3 cut(s)	1056, 1313, 1622
Cfr13I	GGNCC	8 cut(s)	350, 473, 989, 1059, 1124, 1410, 1526, 2072
Cfr9I	CCCGGG	1 cut(s)	1345
CseI	GACGC	1 cut(s)	1284
CsiI	ACCWGGT	1 cut(s)	1864
Csp6I	GTAC	6 cut(s)	479, 562, 760, 1117, 1227, 1808
CspAI	ACCGGT	1 cut(s)	1056
CspCI	CAANNNNNGTGG	2 cut(s)	1606, 1641
CviQI	GTAC	6 cut(s)	479, 562, 760, 1117, 1227, 1808
DdeI	CTNAG	5 cut(s)	35, 75, 226, 346, 999
DraIII	CACNNNGTG	2 cut(s)	743, 1577
DriI	GACNNNNNGTC	1 cut(s)	862
EaeI	YGGCCR	4 cut(s)	237, 437, 643, 1233
Eam1105I	GACNNNNNGTC	1 cut(s)	862
Eco130I	CCWWGG	3 cut(s)	646, 709, 1022
Eco147I	AGGCCT	2 cut(s)	708, 1836
Eco31I	GGTCTC	2 cut(s)	1719, 1874
Eco32I	GATATC	1 cut(s)	1999
Eco47I	GGWCC	6 cut(s)	350, 473, 1059, 1124, 1410, 2072
Eco57I	CTGAAG	1 cut(s)	303
Eco88I	CYCGRG	1 cut(s)	1345
EcoO109I	RGGNCCY	2 cut(s)	350, 1526
EcoRI	GAATTC	1 cut(s)	895
EcoRII	CCWGG	3 cut(s)	1165, 1864, 1992
EcoRV	GATATC	1 cut(s)	1999
EcoT14I	CCWWGG	3 cut(s)	646, 709, 1022
ErhI	CCWWGG	3 cut(s)	646, 709, 1022
FaqI	GGGAC	4 cut(s)	353, 1189, 1423, 1510
FauI	CCCGC	1 cut(s)	664
FbaI	TGATCA	2 cut(s)	400, 1329
FblI	GTMKAC	2 cut(s)	2079, 2088
FokI	GGATG	3 cut(s)	1689, 1783, 2006
FspBI	CTAG	7 cut(s)	69, 630, 788, 1052, 1143, 1470, 2147
GsaI	CCCAGC	1 cut(s)	1547
GsuI	CTGGAG	1 cut(s)	1647
HaeIII	GGCC	9 cut(s)	239, 439, 645, 708, 990, 1235, 1326, 1528, 1836
HgaI	GACGC	1 cut(s)	1284
HincII	GTYRAC	2 cut(s)	856, 2080
HindII	GTYRAC	2 cut(s)	856, 2080
HphI	GGTGA	7 cut(s)	140, 238, 410, 524, 1437, 2008, 2041
Hpy166II	GTNNAC	8 cut(s)	506, 737, 856, 1012, 1153, 1843, 2080, 2089
Hpy188I	TCNGA	9 cut(s)	42, 448, 814, 894, 901, 1002, 1484, 1554, 1772
Hpy8I	GTNNAC	8 cut(s)	506, 737, 856, 1012, 1153, 1843, 2080, 2089
Hpy99I	CGWCG	1 cut(s)	1484
HpyAV	CCTTC	6 cut(s)	363, 1300, 1476, 1995, 2019, 2188
HpyCH4III	ACNGT	9 cut(s)	575, 1006, 1036, 1175, 1226, 1279, 1966, 2059, 2185
HpyCH4IV	ACGT	4 cut(s)	858, 913, 1432, 2067
HpyF10VI	GCNNNNNNNGC	7 cut(s)	668, 705, 743, 752, 932, 941, 2296
HpyF3I	CTNAG	5 cut(s)	35, 75, 226, 346, 999
HpySE526I	ACGT	4 cut(s)	858, 913, 1432, 2067
Kpn2I	TCCGGA	1 cut(s)	1126
Ksp22I	TGATCA	2 cut(s)	400, 1329
LmnI	GCTCC	6 cut(s)	554, 932, 1024, 1129, 1366, 1666
Lsp1109I	GCAGC	8 cut(s)	1027, 1279, 1322, 1579, 1580, 1708, 1771, 1843
LweI	GCATC	2 cut(s)	175, 1711
MabI	ACCWGGT	1 cut(s)	1864
MaeI	CTAG	7 cut(s)	69, 630, 788, 1052, 1143, 1470, 2147
MaeII	ACGT	4 cut(s)	858, 913, 1432, 2067
MboII	GAAGA	3 cut(s)	153, 382, 1468
MfeI	CAATTG	1 cut(s)	828
MflI	RGATCY	3 cut(s)	1026, 1162, 1762
MhlI	GDGCHC	2 cut(s)	739, 748
MlsI	TGGCCA	1 cut(s)	439
MluCI	AATT	7 cut(s)	7, 128, 364, 532, 593, 828, 895
MluNI	TGGCCA	1 cut(s)	439
MlyI	GAGTC	6 cut(s)	203, 452, 1211, 1880, 1925, 2075
MmeI	TCCRAC	3 cut(s)	451, 1462, 2050
Mox20I	TGGCCA	1 cut(s)	439
MroI	TCCGGA	1 cut(s)	1126
MroXI	GAANNNNTTC	1 cut(s)	840
MscI	TGGCCA	1 cut(s)	439
MseI	TTAA	4 cut(s)	456, 1031, 1209, 2154
MslI	CAYNNNNRTG	3 cut(s)	431, 755, 849
Msp20I	TGGCCA	1 cut(s)	439
MspA1I	CMGCKG	2 cut(s)	1292, 1919
MspR9I	CCNGG	8 cut(s)	510, 1167, 1237, 1346, 1347, 1575, 1866, 1994
MunI	CAATTG	1 cut(s)	828
Mva1269I	GAATGC	1 cut(s)	935
MvaI	CCWGG	3 cut(s)	1167, 1866, 1994
MvnI	CGCG	1 cut(s)	331
MwoI	GCNNNNNNNGC	7 cut(s)	668, 705, 743, 752, 932, 941, 2296
NciI	CCSGG	5 cut(s)	510, 1237, 1346, 1347, 1575
NcoI	CCATGG	1 cut(s)	646
NlaIV	GGNNCC	6 cut(s)	556, 721, 1020, 1164, 1411, 1662
NmeAIII	GCCGAG	1 cut(s)	1924
NmuCI	GTSAC	6 cut(s)	133, 252, 796, 1741, 2041, 2059
NspI	RCATGY	2 cut(s)	659, 804
NspV	TTCGAA	1 cut(s)	368
OliI	CACNNNNGTG	1 cut(s)	431
PagI	TCATGA	2 cut(s)	819, 844
PceI	AGGCCT	2 cut(s)	708, 1836
PciI	ACATGT	1 cut(s)	800
PctI	GAATGC	1 cut(s)	935
PdmI	GAANNNNTTC	1 cut(s)	840
PflFI	GACNNNGTC	2 cut(s)	2039, 2084
PflMI	CCANNNNNTGG	1 cut(s)	2263
PfoI	TCCNGGA	1 cut(s)	1992
PinAI	ACCGGT	1 cut(s)	1056
PleI	GAGTC	6 cut(s)	202, 451, 1210, 1880, 1925, 2075
PpsI	GAGTC	6 cut(s)	202, 451, 1210, 1880, 1925, 2075
Ppu21I	YACGTR	1 cut(s)	914
PpuMI	RGGWCCY	1 cut(s)	350
PscI	ACATGT	1 cut(s)	800
Psp1406I	AACGTT	1 cut(s)	2067
Psp5II	RGGWCCY	1 cut(s)	350
Psp6I	CCWGG	3 cut(s)	1165, 1864, 1992
PspFI	CCCAGC	1 cut(s)	1543
PspGI	CCWGG	3 cut(s)	1165, 1864, 1992
PspN4I	GGNNCC	6 cut(s)	556, 721, 1020, 1164, 1411, 1662
PspPI	GGNCC	8 cut(s)	350, 473, 989, 1059, 1124, 1410, 1526, 2072
PspPPI	RGGWCCY	1 cut(s)	350
PstI	CTGCAG	1 cut(s)	1861
PstNI	CAGNNNCTG	3 cut(s)	446, 1040, 2296
PsuI	RGATCY	3 cut(s)	1026, 1162, 1762
PsyI	GACNNNGTC	2 cut(s)	2039, 2084
PvuII	CAGCTG	1 cut(s)	1292
RsaI	GTAC	6 cut(s)	480, 563, 761, 1118, 1228, 1809
RsaNI	GTAC	6 cut(s)	479, 562, 760, 1117, 1227, 1808
RseI	CAYNNNNRTG	3 cut(s)	431, 755, 849
SalI	GTCGAC	1 cut(s)	2078
SaqAI	TTAA	4 cut(s)	456, 1031, 1209, 2154
Sau96I	GGNCC	8 cut(s)	350, 473, 989, 1059, 1124, 1410, 1526, 2072
ScaI	AGTACT	1 cut(s)	563
SchI	GAGTC	6 cut(s)	203, 452, 1211, 1880, 1925, 2075
ScrFI	CCNGG	8 cut(s)	510, 1167, 1237, 1346, 1347, 1575, 1866, 1994
SduI	GDGCHC	2 cut(s)	739, 748
SexAI	ACCWGGT	1 cut(s)	1864
SfaNI	GCATC	2 cut(s)	175, 1711
SfcI	CTRYAG	2 cut(s)	1746, 1857
SfuI	TTCGAA	1 cut(s)	368
SinI	GGWCC	6 cut(s)	350, 473, 1059, 1124, 1410, 2072
SmaI	CCCGGG	1 cut(s)	1347
SmiMI	CAYNNNNRTG	3 cut(s)	431, 755, 849
SmlI	CTYRAG	2 cut(s)	724, 1691
SmoI	CTYRAG	2 cut(s)	724, 1691
SpeI	ACTAGT	2 cut(s)	1142, 2146
Sse9I	AATT	7 cut(s)	7, 128, 364, 532, 593, 828, 895
SseBI	AGGCCT	2 cut(s)	708, 1836
SsiI	CCGC	9 cut(s)	236, 331, 627, 671, 1191, 1658, 1787, 1917, 2111
SspMI	CTAG	7 cut(s)	69, 630, 788, 1052, 1143, 1470, 2147
StuI	AGGCCT	2 cut(s)	708, 1836
StyD4I	CCNGG	8 cut(s)	508, 1165, 1235, 1344, 1345, 1573, 1864, 1992
StyI	CCWWGG	3 cut(s)	646, 709, 1022
TaaI	ACNGT	9 cut(s)	575, 1006, 1036, 1175, 1226, 1279, 1966, 2059, 2185
TaiI	ACGT	4 cut(s)	861, 916, 1435, 2070
TaqI	TCGA	7 cut(s)	214, 368, 883, 1535, 1878, 2079, 2195
TaqII	GACCGA	2 cut(s)	1427, 2074
TasI	AATT	7 cut(s)	7, 128, 364, 532, 593, 828, 895
TatI	WGTACW	3 cut(s)	561, 759, 1116
TauI	GCSGC	2 cut(s)	239, 1661
Tru1I	TTAA	4 cut(s)	456, 1031, 1209, 2154
Tru9I	TTAA	4 cut(s)	456, 1031, 1209, 2154
TscAI	CASTG	3 cut(s)	1041, 1282, 2221
TseFI	GTSAC	6 cut(s)	133, 252, 796, 1741, 2041, 2059
TseI	GCWGC	8 cut(s)	1040, 1292, 1310, 1568, 1592, 1721, 1784, 1856
Tsp45I	GTSAC	6 cut(s)	133, 252, 796, 1741, 2041, 2059
TspDTI	ATGAA	8 cut(s)	77, 330, 511, 669, 795, 833, 836, 1785
TspGWI	ACGGA	3 cut(s)	807, 1806, 2269
TspMI	CCCGGG	1 cut(s)	1345
TspRI	CASTG	3 cut(s)	1041, 1282, 2221
Tth111I	GACNNNGTC	2 cut(s)	2039, 2084
Van91I	CCANNNNNTGG	1 cut(s)	2263
VneI	GTGCAC	1 cut(s)	735
VpaK11BI	GGWCC	6 cut(s)	350, 473, 1059, 1124, 1410, 2072
XapI	RAATTY	3 cut(s)	7, 128, 895
XbaI	TCTAGA	2 cut(s)	1051, 1469
XceI	RCATGY	2 cut(s)	659, 804
XmaI	CCCGGG	1 cut(s)	1345
XmiI	GTMKAC	2 cut(s)	2079, 2088
XmnI	GAANNNNTTC	1 cut(s)	840
XspI	CTAG	7 cut(s)	69, 630, 788, 1052, 1143, 1470, 2147
ZrmI	AGTACT	1 cut(s)	563

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.