subtilisin-like protease
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 139 |
| Acc36I | ACCTGC | 1 cut(s) | 139 |
| AccB1I | GGYRCC | 3 cut(s) | 926, 1952, 2612 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 2555 |
| AccI | GTMKAC | 2 cut(s) | 216, 1410 |
| AccII | CGCG | 2 cut(s) | 66, 1271 |
| AccIII | TCCGGA | 1 cut(s) | 2365 |
| AclWI | GGATC | 5 cut(s) | 575, 2243, 2271, 3004, 3302 |
| AcoI | YGGCCR | 5 cut(s) | 517, 1266, 1646, 1971, 2157 |
| AcsI | RAATTY | 6 cut(s) | 17, 173, 239, 1727, 1835, 3471 |
| AcuI | CTGAAG | 2 cut(s) | 75, 2481 |
| AcyI | GRCGYC | 2 cut(s) | 898, 927 |
| AdeI | CACNNNGTG | 1 cut(s) | 1300 |
| AfaI | GTAC | 5 cut(s) | 165, 534, 621, 781, 3425 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 1702, 2372, 2555, 3147, 3317 |
| AflIII | ACRYGT | 1 cut(s) | 3170 |
| AhlI | ACTAGT | 1 cut(s) | 2941 |
| AjnI | CCWGG | 5 cut(s) | 759, 2159, 2310, 2702, 2776 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1169, 1201 |
| AleI | CACNNNNGTG | 2 cut(s) | 1154, 3525 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2433, 2465 |
| Alw21I | GWGCWC | 5 cut(s) | 807, 859, 921, 1771, 3048 |
| Alw26I | GTCTC | 5 cut(s) | 488, 2878, 2922, 3178, 3270 |
| Alw44I | GTGCAC | 1 cut(s) | 3044 |
| AlwI | GGATC | 5 cut(s) | 575, 2243, 2271, 3004, 3302 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 2021, 2504 |
| Ama87I | CYCGRG | 3 cut(s) | 2724, 2845, 3067 |
| Aor13HI | TCCGGA | 1 cut(s) | 2365 |
| ApaLI | GTGCAC | 1 cut(s) | 3044 |
| ApoI | RAATTY | 6 cut(s) | 17, 173, 239, 1727, 1835, 3471 |
| AspA2I | CCTAGG | 1 cut(s) | 1678 |
| AspLEI | GCGC | 6 cut(s) | 68, 646, 929, 1058, 2209, 2776 |
| AspS9I | GGNCC | 5 cut(s) | 1682, 1924, 2198, 2729, 2966 |
| AsuC2I | CCSGG | 3 cut(s) | 302, 3068, 3069 |
| AsuHPI | GGTGA | 8 cut(s) | 120, 454, 734, 1563, 1619, 1733, 2220, 3512 |
| AsuNHI | GCTAGC | 1 cut(s) | 1880 |
| AvaI | CYCGRG | 3 cut(s) | 2724, 2845, 3067 |
| AvaII | GGWCC | 3 cut(s) | 2198, 2729, 2966 |
| AvrII | CCTAGG | 1 cut(s) | 1678 |
| BaeGI | GKGCMC | 2 cut(s) | 1957, 3048 |
| BaeI | ACNNNNGTAYC | 6 cut(s) | 860, 893, 2418, 2451, 3312, 3345 |
| BalI | TGGCCA | 2 cut(s) | 1648, 2159 |
| BanI | GGYRCC | 3 cut(s) | 926, 1952, 2612 |
| BanII | GRGCYC | 4 cut(s) | 807, 859, 921, 1039 |
| BbsI | GAAGAC | 4 cut(s) | 464, 948, 961, 1052 |
| Bbv12I | GWGCWC | 5 cut(s) | 807, 859, 921, 1771, 3048 |
| BceAI | ACGGC | 6 cut(s) | 241, 914, 1042, 1272, 1795, 1958 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 137, 171, 240, 274, 885, 919 |
| BciT130I | CCWGG | 5 cut(s) | 761, 2161, 2312, 2704, 2778 |
| BciVI | GTATCC | 1 cut(s) | 2574 |
| BclI | TGATCA | 2 cut(s) | 1338, 3517 |
| BcnI | CCSGG | 3 cut(s) | 302, 3068, 3069 |
| BcoDI | GTCTC | 5 cut(s) | 488, 2878, 2922, 3178, 3270 |
| BcuI | ACTAGT | 1 cut(s) | 2941 |
| BfaI | CTAG | 7 cut(s) | 362, 839, 1536, 1679, 1881, 2942, 3532 |
| BfmI | CTRYAG | 2 cut(s) | 1887, 2394 |
| BfoI | RGCGCY | 2 cut(s) | 930, 1059 |
| BfuAI | ACCTGC | 1 cut(s) | 139 |
| BfuI | GTATCC | 1 cut(s) | 2574 |
| BglII | AGATCT | 2 cut(s) | 1182, 1221 |
| BlnI | CCTAGG | 1 cut(s) | 1678 |
| Bme1390I | CCNGG | 8 cut(s) | 302, 761, 2161, 2312, 2704, 2778, 3068, 3069 |
| Bme18I | GGWCC | 3 cut(s) | 2198, 2729, 2966 |
| BmeT110I | CYCGRG | 3 cut(s) | 2724, 2845, 3067 |
| BmgT120I | GGNCC | 5 cut(s) | 1682, 1924, 2198, 2729, 2966 |
| BmiI | GGNNCC | 6 cut(s) | 553, 928, 1954, 2143, 2614, 2730 |
| BmrFI | CCNGG | 8 cut(s) | 302, 761, 2161, 2312, 2704, 2778, 3068, 3069 |
| BmrI | ACTGGG | 3 cut(s) | 79, 800, 1587 |
| BmsI | GCATC | 3 cut(s) | 1843, 2815, 2914 |
| BmtI | GCTAGC | 1 cut(s) | 1884 |
| BmuI | ACTGGG | 3 cut(s) | 79, 800, 1587 |
| BpiI | GAAGAC | 4 cut(s) | 464, 948, 961, 1052 |
| BpmI | CTGGAG | 3 cut(s) | 210, 2394, 2904 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2014 |
| BpuEI | CTTGAG | 3 cut(s) | 1446, 2357, 2741 |
| BpuMI | CCSGG | 3 cut(s) | 302, 3068, 3069 |
| Bsa29I | ATCGAT | 2 cut(s) | 1256, 2855 |
| BsaAI | YACGTR | 1 cut(s) | 994 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1350 |
| BsaHI | GRCGYC | 2 cut(s) | 898, 927 |
| BsaI | GGTCTC | 2 cut(s) | 2922, 3178 |
| BsaWI | WCCGGW | 1 cut(s) | 2365 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 1605, 1635, 2052, 2082, 2950, 2980 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 1702, 2372, 2555, 3147, 3317 |
| Bse118I | RCCGGY | 2 cut(s) | 923, 1892 |
| Bse1I | ACTGG | 9 cut(s) | 85, 787, 795, 1014, 1593, 1685, 1703, 2143, 2814 |
| Bse3DI | GCAATG | 4 cut(s) | 901, 1739, 1744, 2130 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1350 |
| BseAI | TCCGGA | 1 cut(s) | 2365 |
| BseBI | CCWGG | 5 cut(s) | 761, 2161, 2312, 2704, 2778 |
| BseCI | ATCGAT | 2 cut(s) | 1256, 2855 |
| BseGI | GGATG | 8 cut(s) | 73, 89, 375, 1691, 2116, 2122, 2830, 2905 |
| BseJI | GATNNNNATC | 1 cut(s) | 1350 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 1702, 2372, 2555, 3147, 3317 |
| BseMI | GCAATG | 4 cut(s) | 901, 1739, 1744, 2130 |
| BseMII | CTCAG | 5 cut(s) | 1209, 1788, 2028, 2889, 3247 |
| BseNI | ACTGG | 9 cut(s) | 85, 787, 795, 1014, 1593, 1685, 1703, 2143, 2814 |
| BseRI | GAGGAG | 6 cut(s) | 1177, 1180, 1554, 2856, 2882, 2991 |
| BseSI | GKGCMC | 2 cut(s) | 1957, 3048 |
| BseYI | CCCAGC | 1 cut(s) | 268 |
| BsgI | GTGCAG | 3 cut(s) | 1497, 2202, 3111 |
| Bsh1236I | CGCG | 2 cut(s) | 66, 1271 |
| Bsh1285I | CGRYCG | 1 cut(s) | 2473 |
| BshNI | GGYRCC | 3 cut(s) | 926, 1952, 2612 |
| BshVI | ATCGAT | 2 cut(s) | 1256, 2855 |
| BsiEI | CGRYCG | 1 cut(s) | 2473 |
| BsiHKAI | GWGCWC | 5 cut(s) | 807, 859, 921, 1771, 3048 |
| BsiHKCI | CYCGRG | 3 cut(s) | 2724, 2845, 3067 |
| BsiSI | CCGG | 8 cut(s) | 159, 302, 852, 860, 924, 1893, 2366, 3068 |
| BslFI | GGGAC | 6 cut(s) | 283, 807, 1828, 1951, 2742, 3159 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 1702, 2372, 2555, 3147, 3317 |
| BsmAI | GTCTC | 5 cut(s) | 488, 2878, 2922, 3178, 3270 |
| BsmBI | CGTCTC | 1 cut(s) | 488 |
| BsmFI | GGGAC | 6 cut(s) | 283, 807, 1828, 1951, 2742, 3159 |
| BsmI | GAATGC | 2 cut(s) | 1132, 3058 |
| Bso31I | GGTCTC | 2 cut(s) | 2922, 3178 |
| BsoBI | CYCGRG | 3 cut(s) | 2724, 2845, 3067 |
| Bsp1286I | GDGCHC | 7 cut(s) | 807, 859, 921, 1039, 1771, 1957, 3048 |
| Bsp13I | TCCGGA | 1 cut(s) | 2365 |
| Bsp19I | CCATGG | 2 cut(s) | 2430, 3120 |
| BspCNI | CTCAG | 5 cut(s) | 1210, 1789, 2027, 2888, 3246 |
| BspDI | ATCGAT | 2 cut(s) | 1256, 2855 |
| BspEI | TCCGGA | 1 cut(s) | 2365 |
| BspFNI | CGCG | 2 cut(s) | 66, 1271 |
| BspHI | TCATGA | 1 cut(s) | 1354 |
| BspLI | GGNNCC | 6 cut(s) | 553, 928, 1954, 2143, 2614, 2730 |
| BspMAI | CTGCAG | 1 cut(s) | 1891 |
| BspMI | ACCTGC | 1 cut(s) | 139 |
| BspOI | GCTAGC | 1 cut(s) | 1884 |
| BspPI | GGATC | 5 cut(s) | 575, 2243, 2271, 3004, 3302 |
| BspT107I | GGYRCC | 3 cut(s) | 926, 1952, 2612 |
| BspTNI | GGTCTC | 2 cut(s) | 2922, 3178 |
| BsrDI | GCAATG | 4 cut(s) | 901, 1739, 1744, 2130 |
| BsrFI | RCCGGY | 2 cut(s) | 923, 1892 |
| BsrI | ACTGG | 9 cut(s) | 85, 787, 795, 1014, 1593, 1685, 1703, 2143, 2814 |
| BssAI | RCCGGY | 2 cut(s) | 923, 1892 |
| BssNAI | GTATAC | 1 cut(s) | 1411 |
| BssNI | GRCGYC | 2 cut(s) | 898, 927 |
| BssT1I | CCWWGG | 5 cut(s) | 1678, 1927, 2231, 2430, 3120 |
| Bst1107I | GTATAC | 1 cut(s) | 1411 |
| Bst2UI | CCWGG | 5 cut(s) | 761, 2161, 2312, 2704, 2778 |
| Bst6I | CTCTTC | 6 cut(s) | 461, 574, 663, 981, 1154, 1239 |
| BstACI | GRCGYC | 2 cut(s) | 898, 927 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1127 |
| BstBAI | YACGTR | 1 cut(s) | 994 |
| BstDEI | CTNAG | 7 cut(s) | 184, 1218, 1275, 1797, 2014, 2875, 3233 |
| BstDSI | CCRYGG | 5 cut(s) | 1269, 2259, 2430, 3120, 3363 |
| BstF5I | GGATG | 8 cut(s) | 73, 89, 375, 1691, 2116, 2122, 2830, 2905 |
| BstFNI | CGCG | 2 cut(s) | 66, 1271 |
| BstH2I | RGCGCY | 2 cut(s) | 930, 1059 |
| BstHHI | GCGC | 6 cut(s) | 68, 646, 929, 1058, 2209, 2776 |
| BstMAI | GTCTC | 5 cut(s) | 488, 2878, 2922, 3178, 3270 |
| BstMCI | CGRYCG | 1 cut(s) | 2473 |
| BstNI | CCWGG | 5 cut(s) | 761, 2161, 2312, 2704, 2778 |
| BstNSI | RCATGY | 4 cut(s) | 620, 3106, 3174, 3464 |
| BstSCI | CCNGG | 8 cut(s) | 300, 759, 2159, 2310, 2702, 2776, 3066, 3067 |
| BstSFI | CTRYAG | 2 cut(s) | 1887, 2394 |
| BstSLI | GKGCMC | 2 cut(s) | 1957, 3048 |
| BstUI | CGCG | 2 cut(s) | 66, 1271 |
| BstV2I | GAAGAC | 4 cut(s) | 464, 948, 961, 1052 |
| BstX2I | RGATCY | 3 cut(s) | 1182, 1221, 2996 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1698, 3349 |
| BstYI | RGATCY | 3 cut(s) | 1182, 1221, 2996 |
| BstZ17I | GTATAC | 1 cut(s) | 1411 |
| Bsu15I | ATCGAT | 2 cut(s) | 1256, 2855 |
| BsuI | GTATCC | 1 cut(s) | 2574 |
| BsuTUI | ATCGAT | 2 cut(s) | 1256, 2855 |
| BtgI | CCRYGG | 5 cut(s) | 1269, 2259, 2430, 3120, 3363 |
| BtgZI | GCGATG | 1 cut(s) | 13 |
| BtsCI | GGATG | 8 cut(s) | 73, 89, 375, 1691, 2116, 2122, 2830, 2905 |
| BtsI | GCAGTG | 3 cut(s) | 1875, 1884, 2900 |
| BtsIMutI | CAGTG | 7 cut(s) | 718, 1269, 1696, 1875, 1884, 2807, 2900 |
| BveI | ACCTGC | 1 cut(s) | 139 |
| CaiI | CAGNNNCTG | 2 cut(s) | 2021, 2504 |
| CciI | TCATGA | 1 cut(s) | 1354 |
| CfoI | GCGC | 6 cut(s) | 68, 646, 929, 1058, 2209, 2776 |
| Cfr10I | RCCGGY | 2 cut(s) | 923, 1892 |
| Cfr13I | GGNCC | 5 cut(s) | 1682, 1924, 2198, 2729, 2966 |
| Cfr42I | CCGCGG | 1 cut(s) | 1272 |
| Cfr9I | CCCGGG | 1 cut(s) | 3067 |
| ClaI | ATCGAT | 2 cut(s) | 1256, 2855 |
| CseI | GACGC | 1 cut(s) | 906 |
| Csp6I | GTAC | 5 cut(s) | 164, 533, 620, 780, 3424 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1357, 1392 |
| CviQI | GTAC | 5 cut(s) | 164, 533, 620, 780, 3424 |
| DdeI | CTNAG | 7 cut(s) | 184, 1218, 1275, 1797, 2014, 2875, 3233 |
| DinI | GGCGCC | 1 cut(s) | 928 |
| DraIII | CACNNNGTG | 1 cut(s) | 1300 |
| EaeI | YGGCCR | 5 cut(s) | 517, 1266, 1646, 1971, 2157 |
| Eam1104I | CTCTTC | 6 cut(s) | 461, 574, 663, 981, 1154, 1239 |
| EarI | CTCTTC | 6 cut(s) | 461, 574, 663, 981, 1154, 1239 |
| EciI | GGCGGA | 4 cut(s) | 66, 320, 721, 972 |
| Ecl136II | GAGCTC | 3 cut(s) | 805, 857, 919 |
| Eco130I | CCWWGG | 5 cut(s) | 1678, 1927, 2231, 2430, 3120 |
| Eco147I | AGGCCT | 1 cut(s) | 1677 |
| Eco24I | GRGCYC | 4 cut(s) | 807, 859, 921, 1039 |
| Eco31I | GGTCTC | 2 cut(s) | 2922, 3178 |
| Eco32I | GATATC | 2 cut(s) | 844, 2648 |
| Eco47I | GGWCC | 3 cut(s) | 2198, 2729, 2966 |
| Eco53kI | GAGCTC | 3 cut(s) | 805, 857, 919 |
| Eco57I | CTGAAG | 2 cut(s) | 75, 2481 |
| Eco88I | CYCGRG | 3 cut(s) | 2724, 2845, 3067 |
| EcoICRI | GAGCTC | 3 cut(s) | 805, 857, 919 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1924 |
| EcoRI | GAATTC | 2 cut(s) | 1835, 3471 |
| EcoRII | CCWGG | 5 cut(s) | 759, 2159, 2310, 2702, 2776 |
| EcoRV | GATATC | 2 cut(s) | 844, 2648 |
| EcoT14I | CCWWGG | 5 cut(s) | 1678, 1927, 2231, 2430, 3120 |
| EcoT22I | ATGCAT | 1 cut(s) | 3108 |
| EcoT38I | GRGCYC | 4 cut(s) | 807, 859, 921, 1039 |
| EgeI | GGCGCC | 1 cut(s) | 928 |
| EheI | GGCGCC | 1 cut(s) | 928 |
| ErhI | CCWWGG | 5 cut(s) | 1678, 1927, 2231, 2430, 3120 |
| Esp3I | CGTCTC | 1 cut(s) | 488 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 326, 358, 951, 983, 1169, 1201 |
| FaqI | GGGAC | 6 cut(s) | 283, 807, 1828, 1951, 2742, 3159 |
| FauI | CCCGC | 1 cut(s) | 2677 |
| FauNDI | CATATG | 1 cut(s) | 529 |
| FbaI | TGATCA | 2 cut(s) | 1338, 3517 |
| FblI | GTMKAC | 2 cut(s) | 216, 1410 |
| FokI | GGATG | 8 cut(s) | 60, 76, 362, 1678, 2123, 2129, 2837, 2892 |
| FriOI | GRGCYC | 4 cut(s) | 807, 859, 921, 1039 |
| FspBI | CTAG | 7 cut(s) | 362, 839, 1536, 1679, 1881, 2942, 3532 |
| GlaI | GCGC | 6 cut(s) | 67, 645, 928, 1057, 2208, 2775 |
| GsaI | CCCAGC | 1 cut(s) | 272 |
| GsuI | CTGGAG | 3 cut(s) | 210, 2394, 2904 |
| HaeII | RGCGCY | 2 cut(s) | 930, 1059 |
| HapII | CCGG | 8 cut(s) | 159, 302, 852, 860, 924, 1893, 2366, 3068 |
| HgaI | GACGC | 1 cut(s) | 906 |
| HhaI | GCGC | 6 cut(s) | 68, 646, 929, 1058, 2209, 2776 |
| Hin1I | GRCGYC | 2 cut(s) | 898, 927 |
| Hin6I | GCGC | 6 cut(s) | 66, 644, 927, 1056, 2207, 2774 |
| HinP1I | GCGC | 6 cut(s) | 66, 644, 927, 1056, 2207, 2774 |
| HincII | GTYRAC | 2 cut(s) | 2599, 3012 |
| HindII | GTYRAC | 2 cut(s) | 2599, 3012 |
| HindIII | AAGCTT | 3 cut(s) | 1448, 2034, 2353 |
| HpaII | CCGG | 8 cut(s) | 159, 302, 852, 860, 924, 1893, 2366, 3068 |
| HphI | GGTGA | 8 cut(s) | 120, 454, 734, 1563, 1619, 1733, 2220, 3512 |
| Hpy188III | TCNNGA | 9 cut(s) | 1355, 1434, 2016, 2080, 2366, 2373, 2439, 2847, 3385 |
| Hpy99I | CGWCG | 7 cut(s) | 49, 52, 262, 290, 480, 483, 900 |
| HpyAV | CCTTC | 8 cut(s) | 546, 977, 1584, 1667, 1696, 2211, 3198, 3222 |
| HpyCH4IV | ACGT | 3 cut(s) | 993, 1771, 2122 |
| HpyF3I | CTNAG | 7 cut(s) | 184, 1218, 1275, 1797, 2014, 2875, 3233 |
| HpySE526I | ACGT | 3 cut(s) | 993, 1771, 2122 |
| Hsp92I | GRCGYC | 2 cut(s) | 898, 927 |
| HspAI | GCGC | 6 cut(s) | 66, 644, 927, 1056, 2207, 2774 |
| KasI | GGCGCC | 1 cut(s) | 926 |
| Kpn2I | TCCGGA | 1 cut(s) | 2365 |
| KroI | GCCGGC | 1 cut(s) | 923 |
| KroNI | GCCGGC | 1 cut(s) | 925 |
| Ksp22I | TGATCA | 2 cut(s) | 1338, 3517 |
| KspI | CCGCGG | 1 cut(s) | 1272 |
| LmnI | GCTCC | 7 cut(s) | 64, 748, 854, 862, 1766, 2018, 2147 |
| LweI | GCATC | 3 cut(s) | 1843, 2815, 2914 |
| MaeI | CTAG | 7 cut(s) | 362, 839, 1536, 1679, 1881, 2942, 3532 |
| MaeII | ACGT | 3 cut(s) | 993, 1771, 2122 |
| MfeI | CAATTG | 2 cut(s) | 2679, 3303 |
| MflI | RGATCY | 3 cut(s) | 1182, 1221, 2996 |
| MhlI | GDGCHC | 7 cut(s) | 807, 859, 921, 1039, 1771, 1957, 3048 |
| MlsI | TGGCCA | 2 cut(s) | 1648, 2159 |
| MluNI | TGGCCA | 2 cut(s) | 1648, 2159 |
| Mly113I | GGCGCC | 1 cut(s) | 927 |
| MlyI | GAGTC | 6 cut(s) | 305, 317, 1509, 1661, 2378, 3272 |
| MmeI | TCCRAC | 3 cut(s) | 309, 1357, 2573 |
| Mox20I | TGGCCA | 2 cut(s) | 1648, 2159 |
| Mph1103I | ATGCAT | 1 cut(s) | 3108 |
| MreI | CGCCGGCG | 1 cut(s) | 923 |
| MroI | TCCGGA | 1 cut(s) | 2365 |
| MroNI | GCCGGC | 1 cut(s) | 923 |
| MscI | TGGCCA | 2 cut(s) | 1648, 2159 |
| MseI | TTAA | 3 cut(s) | 1731, 2421, 3204 |
| MslI | CAYNNNNRTG | 4 cut(s) | 1154, 1863, 3051, 3525 |
| Msp20I | TGGCCA | 2 cut(s) | 1648, 2159 |
| MspA1I | CMGCKG | 4 cut(s) | 1101, 1271, 2186, 2501 |
| MspI | CCGG | 8 cut(s) | 159, 302, 852, 860, 924, 1893, 2366, 3068 |
| MspR9I | CCNGG | 8 cut(s) | 302, 761, 2161, 2312, 2704, 2778, 3068, 3069 |
| MunI | CAATTG | 2 cut(s) | 2679, 3303 |
| Mva1269I | GAATGC | 2 cut(s) | 1132, 3058 |
| MvaI | CCWGG | 5 cut(s) | 761, 2161, 2312, 2704, 2778 |
| MvnI | CGCG | 2 cut(s) | 66, 1271 |
| NaeI | GCCGGC | 1 cut(s) | 925 |
| NarI | GGCGCC | 1 cut(s) | 927 |
| NciI | CCSGG | 3 cut(s) | 302, 3068, 3069 |
| NcoI | CCATGG | 2 cut(s) | 2430, 3120 |
| NdeI | CATATG | 1 cut(s) | 529 |
| NgoMIV | GCCGGC | 1 cut(s) | 923 |
| NheI | GCTAGC | 1 cut(s) | 1880 |
| NlaIV | GGNNCC | 6 cut(s) | 553, 928, 1954, 2143, 2614, 2730 |
| NmeAIII | GCCGAG | 1 cut(s) | 3016 |
| NmuCI | GTSAC | 6 cut(s) | 460, 1903, 2073, 2944, 3262, 3527 |
| NsiI | ATGCAT | 1 cut(s) | 3108 |
| NspI | RCATGY | 4 cut(s) | 620, 3106, 3174, 3464 |
| OliI | CACNNNNGTG | 2 cut(s) | 1154, 3525 |
| PaeI | GCATGC | 1 cut(s) | 3464 |
| PagI | TCATGA | 1 cut(s) | 1354 |
| PaqCI | CACCTGC | 1 cut(s) | 139 |
| PceI | AGGCCT | 1 cut(s) | 1677 |
| PciI | ACATGT | 1 cut(s) | 3170 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 927, 1049, 2852 |
| PctI | GAATGC | 2 cut(s) | 1132, 3058 |
| PdiI | GCCGGC | 1 cut(s) | 925 |
| PfeI | GAWTC | 8 cut(s) | 330, 504, 1105, 1346, 1715, 2408, 2545, 2813 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 2555 |
| PleI | GAGTC | 6 cut(s) | 304, 316, 1508, 1660, 2377, 3272 |
| PluTI | GGCGCC | 1 cut(s) | 930 |
| PpsI | GAGTC | 6 cut(s) | 304, 316, 1508, 1660, 2377, 3272 |
| Ppu21I | YACGTR | 1 cut(s) | 994 |
| PscI | ACATGT | 1 cut(s) | 3170 |
| Psp124BI | GAGCTC | 3 cut(s) | 807, 859, 921 |
| Psp6I | CCWGG | 5 cut(s) | 759, 2159, 2310, 2702, 2776 |
| PspFI | CCCAGC | 1 cut(s) | 268 |
| PspGI | CCWGG | 5 cut(s) | 759, 2159, 2310, 2702, 2776 |
| PspN4I | GGNNCC | 6 cut(s) | 553, 928, 1954, 2143, 2614, 2730 |
| PspPI | GGNCC | 5 cut(s) | 1682, 1924, 2198, 2729, 2966 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 3407, 3439 |
| PstI | CTGCAG | 1 cut(s) | 1891 |
| PstNI | CAGNNNCTG | 2 cut(s) | 2021, 2504 |
| PsuI | RGATCY | 3 cut(s) | 1182, 1221, 2996 |
| PvuII | CAGCTG | 2 cut(s) | 2186, 2501 |
| RsaI | GTAC | 5 cut(s) | 165, 534, 621, 781, 3425 |
| RsaNI | GTAC | 5 cut(s) | 164, 533, 620, 780, 3424 |
| RseI | CAYNNNNRTG | 4 cut(s) | 1154, 1863, 3051, 3525 |
| SacI | GAGCTC | 3 cut(s) | 807, 859, 921 |
| SacII | CCGCGG | 1 cut(s) | 1272 |
| SaqAI | TTAA | 3 cut(s) | 1731, 2421, 3204 |
| Sau96I | GGNCC | 5 cut(s) | 1682, 1924, 2198, 2729, 2966 |
| SchI | GAGTC | 6 cut(s) | 305, 317, 1509, 1661, 2378, 3272 |
| ScrFI | CCNGG | 8 cut(s) | 302, 761, 2161, 2312, 2704, 2778, 3068, 3069 |
| SduI | GDGCHC | 7 cut(s) | 807, 859, 921, 1039, 1771, 1957, 3048 |
| SfaNI | GCATC | 3 cut(s) | 1843, 2815, 2914 |
| SfcI | CTRYAG | 2 cut(s) | 1887, 2394 |
| SfoI | GGCGCC | 1 cut(s) | 928 |
| Sfr303I | CCGCGG | 1 cut(s) | 1272 |
| SgrAI | CRCCGGYG | 1 cut(s) | 923 |
| SgrBI | CCGCGG | 1 cut(s) | 1272 |
| SinI | GGWCC | 3 cut(s) | 2198, 2729, 2966 |
| SmaI | CCCGGG | 1 cut(s) | 3069 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 1154, 1863, 3051, 3525 |
| SmlI | CTYRAG | 3 cut(s) | 1461, 2336, 2756 |
| SmoI | CTYRAG | 3 cut(s) | 1461, 2336, 2756 |
| SpeI | ACTAGT | 1 cut(s) | 2941 |
| SphI | GCATGC | 1 cut(s) | 3464 |
| SseBI | AGGCCT | 1 cut(s) | 1677 |
| SspDI | GGCGCC | 1 cut(s) | 926 |
| SspI | AATATT | 2 cut(s) | 1585, 2697 |
| SspMI | CTAG | 7 cut(s) | 362, 839, 1536, 1679, 1881, 2942, 3532 |
| SstI | GAGCTC | 3 cut(s) | 807, 859, 921 |
| StuI | AGGCCT | 1 cut(s) | 1677 |
| StyD4I | CCNGG | 8 cut(s) | 300, 759, 2159, 2310, 2702, 2776, 3066, 3067 |
| StyI | CCWWGG | 5 cut(s) | 1678, 1927, 2231, 2430, 3120 |
| TaiI | ACGT | 3 cut(s) | 996, 1774, 2125 |
| TaqII | GACCGA | 1 cut(s) | 2459 |
| TauI | GCSGC | 4 cut(s) | 706, 1101, 1271, 1274 |
| TfiI | GAWTC | 8 cut(s) | 330, 504, 1105, 1346, 1715, 2408, 2545, 2813 |
| Tru1I | TTAA | 3 cut(s) | 1731, 2421, 3204 |
| Tru9I | TTAA | 3 cut(s) | 1731, 2421, 3204 |
| TscAI | CASTG | 7 cut(s) | 718, 1269, 1703, 1882, 1891, 2814, 2907 |
| TseFI | GTSAC | 6 cut(s) | 460, 1903, 2073, 2944, 3262, 3527 |
| Tsp45I | GTSAC | 6 cut(s) | 460, 1903, 2073, 2944, 3262, 3527 |
| TspGWI | ACGGA | 6 cut(s) | 301, 1358, 1469, 2276, 3277, 3465 |
| TspMI | CCCGGG | 1 cut(s) | 3067 |
| TspRI | CASTG | 7 cut(s) | 718, 1269, 1703, 1882, 1891, 2814, 2907 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 2555 |
| VneI | GTGCAC | 1 cut(s) | 3044 |
| VpaK11BI | GGWCC | 3 cut(s) | 2198, 2729, 2966 |
| XapI | RAATTY | 6 cut(s) | 17, 173, 239, 1727, 1835, 3471 |
| XceI | RCATGY | 4 cut(s) | 620, 3106, 3174, 3464 |
| XcmI | CCANNNNNNNNNTGG | 4 cut(s) | 323, 659, 1071, 1600 |
| XmaI | CCCGGG | 1 cut(s) | 3067 |
| XmaJI | CCTAGG | 1 cut(s) | 1678 |
| XmiI | GTMKAC | 2 cut(s) | 216, 1410 |
| XspI | CTAG | 7 cut(s) | 362, 839, 1536, 1679, 1881, 2942, 3532 |
| Zsp2I | ATGCAT | 1 cut(s) | 3108 |