subtilisin-like protease
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 806, 1466 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1409 |
| AccI | GTMKAC | 1 cut(s) | 264 |
| AccIII | TCCGGA | 1 cut(s) | 1219 |
| AciI | CCGC | 5 cut(s) | 61, 858, 1374, 1538, 1593 |
| AclWI | GGATC | 4 cut(s) | 1097, 1125, 1858, 2156 |
| AcoI | YGGCCR | 4 cut(s) | 68, 500, 825, 1011 |
| AcsI | RAATTY | 4 cut(s) | 46, 581, 689, 2325 |
| AcuI | CTGAAG | 1 cut(s) | 1335 |
| AdeI | CACNNNGTG | 1 cut(s) | 154 |
| AfaI | GTAC | 1 cut(s) | 2279 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 556, 1226, 1409, 2001, 2171 |
| AflIII | ACRYGT | 1 cut(s) | 2024 |
| AgsI | TTSAA | 9 cut(s) | 432, 520, 634, 670, 1044, 1238, 1448, 1519, 2212 |
| AhlI | ACTAGT | 1 cut(s) | 1795 |
| AjnI | CCWGG | 4 cut(s) | 1013, 1164, 1556, 1630 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 113, 145 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1287, 1319 |
| Alw21I | GWGCWC | 3 cut(s) | 9, 625, 1902 |
| Alw26I | GTCTC | 5 cut(s) | 17, 1732, 1776, 2032, 2124 |
| Alw44I | GTGCAC | 1 cut(s) | 1898 |
| AlwI | GGATC | 4 cut(s) | 1097, 1125, 1858, 2156 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 875, 1358 |
| Ama87I | CYCGRG | 3 cut(s) | 1578, 1699, 1921 |
| Aor13HI | TCCGGA | 1 cut(s) | 1219 |
| ApaLI | GTGCAC | 1 cut(s) | 1898 |
| ApoI | RAATTY | 4 cut(s) | 46, 581, 689, 2325 |
| AspA2I | CCTAGG | 1 cut(s) | 532 |
| AspLEI | GCGC | 2 cut(s) | 1063, 1630 |
| AspS9I | GGNCC | 5 cut(s) | 536, 778, 1052, 1583, 1820 |
| AsuC2I | CCSGG | 2 cut(s) | 1922, 1923 |
| AsuHPI | GGTGA | 4 cut(s) | 417, 473, 587, 1074 |
| AsuNHI | GCTAGC | 1 cut(s) | 734 |
| AvaI | CYCGRG | 3 cut(s) | 1578, 1699, 1921 |
| AvaII | GGWCC | 3 cut(s) | 1052, 1583, 1820 |
| AvrII | CCTAGG | 1 cut(s) | 532 |
| BaeGI | GKGCMC | 2 cut(s) | 811, 1902 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 1272, 1305, 2166, 2199 |
| BalI | TGGCCA | 2 cut(s) | 502, 1013 |
| BanI | GGYRCC | 2 cut(s) | 806, 1466 |
| BanII | GRGCYC | 1 cut(s) | 9 |
| Bbv12I | GWGCWC | 3 cut(s) | 9, 625, 1902 |
| BceAI | ACGGC | 2 cut(s) | 649, 812 |
| BciT130I | CCWGG | 4 cut(s) | 1015, 1166, 1558, 1632 |
| BciVI | GTATCC | 1 cut(s) | 1428 |
| BclI | TGATCA | 2 cut(s) | 192, 2371 |
| BcnI | CCSGG | 2 cut(s) | 1922, 1923 |
| BcoDI | GTCTC | 5 cut(s) | 17, 1732, 1776, 2032, 2124 |
| BcuI | ACTAGT | 1 cut(s) | 1795 |
| BfaI | CTAG | 4 cut(s) | 390, 533, 735, 1796 |
| BfmI | CTRYAG | 2 cut(s) | 741, 1248 |
| BfuI | GTATCC | 1 cut(s) | 1428 |
| BglII | AGATCT | 1 cut(s) | 126 |
| BlnI | CCTAGG | 1 cut(s) | 532 |
| Bme1390I | CCNGG | 6 cut(s) | 1015, 1166, 1558, 1632, 1922, 1923 |
| Bme18I | GGWCC | 3 cut(s) | 1052, 1583, 1820 |
| BmeT110I | CYCGRG | 3 cut(s) | 1578, 1699, 1921 |
| BmgT120I | GGNCC | 5 cut(s) | 536, 778, 1052, 1583, 1820 |
| BmiI | GGNNCC | 4 cut(s) | 808, 997, 1468, 1584 |
| BmrFI | CCNGG | 6 cut(s) | 1015, 1166, 1558, 1632, 1922, 1923 |
| BmrI | ACTGGG | 1 cut(s) | 441 |
| BmsI | GCATC | 3 cut(s) | 697, 1669, 1768 |
| BmtI | GCTAGC | 1 cut(s) | 738 |
| BmuI | ACTGGG | 1 cut(s) | 441 |
| BpmI | CTGGAG | 2 cut(s) | 1248, 1758 |
| Bpu10I | CCTNAGC | 1 cut(s) | 868 |
| BpuEI | CTTGAG | 3 cut(s) | 300, 1211, 1595 |
| BpuMI | CCSGG | 2 cut(s) | 1922, 1923 |
| Bsa29I | ATCGAT | 1 cut(s) | 1709 |
| BsaBI | GATNNNNATC | 1 cut(s) | 204 |
| BsaI | GGTCTC | 2 cut(s) | 1776, 2032 |
| BsaWI | WCCGGW | 1 cut(s) | 1219 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 459, 489, 906, 936, 1804, 1834 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 556, 1226, 1409, 2001, 2171 |
| Bse118I | RCCGGY | 1 cut(s) | 746 |
| Bse1I | ACTGG | 5 cut(s) | 447, 539, 557, 997, 1668 |
| Bse3DI | GCAATG | 3 cut(s) | 593, 598, 984 |
| Bse8I | GATNNNNATC | 1 cut(s) | 204 |
| BseAI | TCCGGA | 1 cut(s) | 1219 |
| BseBI | CCWGG | 4 cut(s) | 1015, 1166, 1558, 1632 |
| BseCI | ATCGAT | 1 cut(s) | 1709 |
| BseGI | GGATG | 5 cut(s) | 545, 970, 976, 1684, 1759 |
| BseJI | GATNNNNATC | 1 cut(s) | 204 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 556, 1226, 1409, 2001, 2171 |
| BseMI | GCAATG | 3 cut(s) | 593, 598, 984 |
| BseMII | CTCAG | 4 cut(s) | 642, 882, 1743, 2101 |
| BseNI | ACTGG | 5 cut(s) | 447, 539, 557, 997, 1668 |
| BseRI | GAGGAG | 6 cut(s) | 5, 124, 408, 1710, 1736, 1845 |
| BseSI | GKGCMC | 2 cut(s) | 811, 1902 |
| BsgI | GTGCAG | 3 cut(s) | 351, 1056, 1965 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1327 |
| BshNI | GGYRCC | 2 cut(s) | 806, 1466 |
| BshVI | ATCGAT | 1 cut(s) | 1709 |
| BsiEI | CGRYCG | 1 cut(s) | 1327 |
| BsiHKAI | GWGCWC | 3 cut(s) | 9, 625, 1902 |
| BsiHKCI | CYCGRG | 3 cut(s) | 1578, 1699, 1921 |
| BsiSI | CCGG | 3 cut(s) | 747, 1220, 1922 |
| BslFI | GGGAC | 4 cut(s) | 682, 805, 1596, 2013 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 556, 1226, 1409, 2001, 2171 |
| BsmAI | GTCTC | 5 cut(s) | 17, 1732, 1776, 2032, 2124 |
| BsmBI | CGTCTC | 1 cut(s) | 17 |
| BsmFI | GGGAC | 4 cut(s) | 682, 805, 1596, 2013 |
| BsmI | GAATGC | 1 cut(s) | 1912 |
| Bso31I | GGTCTC | 2 cut(s) | 1776, 2032 |
| BsoBI | CYCGRG | 3 cut(s) | 1578, 1699, 1921 |
| Bsp1286I | GDGCHC | 4 cut(s) | 9, 625, 811, 1902 |
| Bsp13I | TCCGGA | 1 cut(s) | 1219 |
| Bsp143I | GATC | 9 cut(s) | 126, 192, 1089, 1117, 1490, 1787, 1850, 2161, 2371 |
| Bsp19I | CCATGG | 2 cut(s) | 1284, 1974 |
| BspACI | CCGC | 5 cut(s) | 61, 858, 1374, 1538, 1593 |
| BspCNI | CTCAG | 4 cut(s) | 643, 881, 1742, 2100 |
| BspDI | ATCGAT | 1 cut(s) | 1709 |
| BspEI | TCCGGA | 1 cut(s) | 1219 |
| BspHI | TCATGA | 1 cut(s) | 208 |
| BspLI | GGNNCC | 4 cut(s) | 808, 997, 1468, 1584 |
| BspMAI | CTGCAG | 1 cut(s) | 745 |
| BspOI | GCTAGC | 1 cut(s) | 738 |
| BspPI | GGATC | 4 cut(s) | 1097, 1125, 1858, 2156 |
| BspT107I | GGYRCC | 2 cut(s) | 806, 1466 |
| BspTNI | GGTCTC | 2 cut(s) | 1776, 2032 |
| BsrDI | GCAATG | 3 cut(s) | 593, 598, 984 |
| BsrFI | RCCGGY | 1 cut(s) | 746 |
| BsrI | ACTGG | 5 cut(s) | 447, 539, 557, 997, 1668 |
| BssAI | RCCGGY | 1 cut(s) | 746 |
| BssMI | GATC | 9 cut(s) | 126, 192, 1089, 1117, 1490, 1787, 1850, 2161, 2371 |
| BssNAI | GTATAC | 1 cut(s) | 265 |
| BssT1I | CCWWGG | 5 cut(s) | 532, 781, 1085, 1284, 1974 |
| Bst1107I | GTATAC | 1 cut(s) | 265 |
| Bst2UI | CCWGG | 4 cut(s) | 1015, 1166, 1558, 1632 |
| BstC8I | GCNNGC | 7 cut(s) | 219, 736, 1108, 1372, 1540, 1544, 2316 |
| BstDEI | CTNAG | 4 cut(s) | 651, 868, 1729, 2087 |
| BstDSI | CCRYGG | 4 cut(s) | 1113, 1284, 1974, 2217 |
| BstF5I | GGATG | 5 cut(s) | 545, 970, 976, 1684, 1759 |
| BstHHI | GCGC | 2 cut(s) | 1063, 1630 |
| BstKTI | GATC | 9 cut(s) | 129, 195, 1092, 1120, 1493, 1790, 1853, 2164, 2374 |
| BstMAI | GTCTC | 5 cut(s) | 17, 1732, 1776, 2032, 2124 |
| BstMBI | GATC | 9 cut(s) | 126, 192, 1089, 1117, 1490, 1787, 1850, 2161, 2371 |
| BstMCI | CGRYCG | 1 cut(s) | 1327 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 67, 740, 815, 824, 864, 1355, 1380, 1562, 1601 |
| BstNI | CCWGG | 4 cut(s) | 1015, 1166, 1558, 1632 |
| BstNSI | RCATGY | 3 cut(s) | 1960, 2028, 2318 |
| BstSCI | CCNGG | 6 cut(s) | 1013, 1164, 1556, 1630, 1920, 1921 |
| BstSFI | CTRYAG | 2 cut(s) | 741, 1248 |
| BstSLI | GKGCMC | 2 cut(s) | 811, 1902 |
| BstX2I | RGATCY | 2 cut(s) | 126, 1850 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 552, 2203 |
| BstYI | RGATCY | 2 cut(s) | 126, 1850 |
| BstZ17I | GTATAC | 1 cut(s) | 265 |
| Bsu15I | ATCGAT | 1 cut(s) | 1709 |
| BsuI | GTATCC | 1 cut(s) | 1428 |
| BsuTUI | ATCGAT | 1 cut(s) | 1709 |
| BtgI | CCRYGG | 4 cut(s) | 1113, 1284, 1974, 2217 |
| BtsCI | GGATG | 5 cut(s) | 545, 970, 976, 1684, 1759 |
| BtsI | GCAGTG | 3 cut(s) | 729, 738, 1754 |
| BtsIMutI | CAGTG | 7 cut(s) | 104, 550, 729, 738, 1661, 1754, 2374 |
| Cac8I | GCNNGC | 7 cut(s) | 219, 736, 1108, 1372, 1540, 1544, 2316 |
| CaiI | CAGNNNCTG | 2 cut(s) | 875, 1358 |
| CciI | TCATGA | 1 cut(s) | 208 |
| CfoI | GCGC | 2 cut(s) | 1063, 1630 |
| Cfr10I | RCCGGY | 1 cut(s) | 746 |
| Cfr13I | GGNCC | 5 cut(s) | 536, 778, 1052, 1583, 1820 |
| Cfr9I | CCCGGG | 1 cut(s) | 1921 |
| ClaI | ATCGAT | 1 cut(s) | 1709 |
| Csp6I | GTAC | 1 cut(s) | 2278 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 211, 246 |
| CviQI | GTAC | 1 cut(s) | 2278 |
| DdeI | CTNAG | 4 cut(s) | 651, 868, 1729, 2087 |
| DpnI | GATC | 9 cut(s) | 128, 194, 1091, 1119, 1492, 1789, 1852, 2163, 2373 |
| DpnII | GATC | 9 cut(s) | 126, 192, 1089, 1117, 1490, 1787, 1850, 2161, 2371 |
| DraIII | CACNNNGTG | 1 cut(s) | 154 |
| EaeI | YGGCCR | 4 cut(s) | 68, 500, 825, 1011 |
| Ecl136II | GAGCTC | 1 cut(s) | 7 |
| Eco130I | CCWWGG | 5 cut(s) | 532, 781, 1085, 1284, 1974 |
| Eco147I | AGGCCT | 1 cut(s) | 531 |
| Eco24I | GRGCYC | 1 cut(s) | 9 |
| Eco31I | GGTCTC | 2 cut(s) | 1776, 2032 |
| Eco32I | GATATC | 1 cut(s) | 1502 |
| Eco47I | GGWCC | 3 cut(s) | 1052, 1583, 1820 |
| Eco53kI | GAGCTC | 1 cut(s) | 7 |
| Eco57I | CTGAAG | 1 cut(s) | 1335 |
| Eco88I | CYCGRG | 3 cut(s) | 1578, 1699, 1921 |
| EcoICRI | GAGCTC | 1 cut(s) | 7 |
| EcoO109I | RGGNCCY | 1 cut(s) | 778 |
| EcoRI | GAATTC | 2 cut(s) | 689, 2325 |
| EcoRII | CCWGG | 4 cut(s) | 1013, 1164, 1556, 1630 |
| EcoRV | GATATC | 1 cut(s) | 1502 |
| EcoT14I | CCWWGG | 5 cut(s) | 532, 781, 1085, 1284, 1974 |
| EcoT22I | ATGCAT | 1 cut(s) | 1962 |
| EcoT38I | GRGCYC | 1 cut(s) | 9 |
| ErhI | CCWWGG | 5 cut(s) | 532, 781, 1085, 1284, 1974 |
| Esp3I | CGTCTC | 1 cut(s) | 17 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 113, 145 |
| FaqI | GGGAC | 4 cut(s) | 682, 805, 1596, 2013 |
| FauI | CCCGC | 1 cut(s) | 1531 |
| FbaI | TGATCA | 2 cut(s) | 192, 2371 |
| FblI | GTMKAC | 1 cut(s) | 264 |
| FokI | GGATG | 5 cut(s) | 532, 977, 983, 1691, 1746 |
| FriOI | GRGCYC | 1 cut(s) | 9 |
| FspBI | CTAG | 4 cut(s) | 390, 533, 735, 1796 |
| GlaI | GCGC | 2 cut(s) | 1062, 1629 |
| GsuI | CTGGAG | 2 cut(s) | 1248, 1758 |
| HapII | CCGG | 3 cut(s) | 747, 1220, 1922 |
| HhaI | GCGC | 2 cut(s) | 1063, 1630 |
| Hin6I | GCGC | 2 cut(s) | 1061, 1628 |
| HinP1I | GCGC | 2 cut(s) | 1061, 1628 |
| HincII | GTYRAC | 2 cut(s) | 1453, 1866 |
| HindII | GTYRAC | 2 cut(s) | 1453, 1866 |
| HindIII | AAGCTT | 3 cut(s) | 302, 888, 1207 |
| HinfI | GANTC | 9 cut(s) | 200, 354, 506, 569, 1223, 1262, 1399, 1667, 2132 |
| HpaII | CCGG | 3 cut(s) | 747, 1220, 1922 |
| HphI | GGTGA | 4 cut(s) | 417, 473, 587, 1074 |
| Hpy166II | GTNNAC | 9 cut(s) | 148, 265, 832, 1182, 1453, 1639, 1832, 1866, 1900 |
| Hpy188I | TCNGA | 8 cut(s) | 253, 620, 679, 964, 1074, 1490, 2090, 2226 |
| Hpy188III | TCNNGA | 9 cut(s) | 209, 288, 870, 934, 1220, 1227, 1293, 1701, 2239 |
| Hpy8I | GTNNAC | 9 cut(s) | 148, 265, 832, 1182, 1453, 1639, 1832, 1866, 1900 |
| HpyAV | CCTTC | 6 cut(s) | 438, 521, 550, 1065, 2052, 2076 |
| HpyCH4IV | ACGT | 2 cut(s) | 625, 976 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 67, 740, 815, 824, 864, 1355, 1380, 1562, 1601 |
| HpyF3I | CTNAG | 4 cut(s) | 651, 868, 1729, 2087 |
| HpySE526I | ACGT | 2 cut(s) | 625, 976 |
| HspAI | GCGC | 2 cut(s) | 1061, 1628 |
| Kpn2I | TCCGGA | 1 cut(s) | 1219 |
| Ksp22I | TGATCA | 2 cut(s) | 192, 2371 |
| Kzo9I | GATC | 9 cut(s) | 126, 192, 1089, 1117, 1490, 1787, 1850, 2161, 2371 |
| LmnI | GCTCC | 6 cut(s) | 4, 12, 111, 620, 872, 1001 |
| LweI | GCATC | 3 cut(s) | 697, 1669, 1768 |
| MaeI | CTAG | 4 cut(s) | 390, 533, 735, 1796 |
| MaeII | ACGT | 2 cut(s) | 625, 976 |
| MaeIII | GTNAC | 8 cut(s) | 269, 757, 770, 927, 1339, 1798, 2104, 2116 |
| MalI | GATC | 9 cut(s) | 128, 194, 1091, 1119, 1492, 1789, 1852, 2163, 2373 |
| MboI | GATC | 9 cut(s) | 126, 192, 1089, 1117, 1490, 1787, 1850, 2161, 2371 |
| MboII | GAAGA | 5 cut(s) | 55, 133, 136, 434, 536 |
| MfeI | CAATTG | 2 cut(s) | 1533, 2157 |
| MflI | RGATCY | 2 cut(s) | 126, 1850 |
| MhlI | GDGCHC | 4 cut(s) | 9, 625, 811, 1902 |
| MlsI | TGGCCA | 2 cut(s) | 502, 1013 |
| MluNI | TGGCCA | 2 cut(s) | 502, 1013 |
| MlyI | GAGTC | 4 cut(s) | 363, 515, 1232, 2126 |
| MmeI | TCCRAC | 2 cut(s) | 211, 1427 |
| Mox20I | TGGCCA | 2 cut(s) | 502, 1013 |
| Mph1103I | ATGCAT | 1 cut(s) | 1962 |
| MroI | TCCGGA | 1 cut(s) | 1219 |
| MscI | TGGCCA | 2 cut(s) | 502, 1013 |
| MseI | TTAA | 3 cut(s) | 585, 1275, 2058 |
| MslI | CAYNNNNRTG | 2 cut(s) | 717, 1905 |
| Msp20I | TGGCCA | 2 cut(s) | 502, 1013 |
| MspA1I | CMGCKG | 1 cut(s) | 1355 |
| MspI | CCGG | 3 cut(s) | 747, 1220, 1922 |
| MspR9I | CCNGG | 6 cut(s) | 1015, 1166, 1558, 1632, 1922, 1923 |
| MunI | CAATTG | 2 cut(s) | 1533, 2157 |
| Mva1269I | GAATGC | 1 cut(s) | 1912 |
| MvaI | CCWGG | 4 cut(s) | 1015, 1166, 1558, 1632 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 67, 740, 815, 824, 864, 1355, 1380, 1562, 1601 |
| NciI | CCSGG | 2 cut(s) | 1922, 1923 |
| NcoI | CCATGG | 2 cut(s) | 1284, 1974 |
| NdeII | GATC | 9 cut(s) | 126, 192, 1089, 1117, 1490, 1787, 1850, 2161, 2371 |
| NheI | GCTAGC | 1 cut(s) | 734 |
| NlaIV | GGNNCC | 4 cut(s) | 808, 997, 1468, 1584 |
| NmeAIII | GCCGAG | 1 cut(s) | 1870 |
| NmuCI | GTSAC | 4 cut(s) | 757, 927, 1798, 2116 |
| NsiI | ATGCAT | 1 cut(s) | 1962 |
| NspI | RCATGY | 3 cut(s) | 1960, 2028, 2318 |
| PaeI | GCATGC | 1 cut(s) | 2318 |
| PagI | TCATGA | 1 cut(s) | 208 |
| PceI | AGGCCT | 1 cut(s) | 531 |
| PciI | ACATGT | 1 cut(s) | 2024 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1706 |
| PctI | GAATGC | 1 cut(s) | 1912 |
| PfeI | GAWTC | 5 cut(s) | 200, 569, 1262, 1399, 1667 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1409 |
| PleI | GAGTC | 4 cut(s) | 362, 514, 1231, 2126 |
| PpsI | GAGTC | 4 cut(s) | 362, 514, 1231, 2126 |
| PscI | ACATGT | 1 cut(s) | 2024 |
| Psp124BI | GAGCTC | 1 cut(s) | 9 |
| Psp6I | CCWGG | 4 cut(s) | 1013, 1164, 1556, 1630 |
| PspGI | CCWGG | 4 cut(s) | 1013, 1164, 1556, 1630 |
| PspN4I | GGNNCC | 4 cut(s) | 808, 997, 1468, 1584 |
| PspPI | GGNCC | 5 cut(s) | 536, 778, 1052, 1583, 1820 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 2261, 2293 |
| PstI | CTGCAG | 1 cut(s) | 745 |
| PstNI | CAGNNNCTG | 2 cut(s) | 875, 1358 |
| PsuI | RGATCY | 2 cut(s) | 126, 1850 |
| PvuII | CAGCTG | 1 cut(s) | 1355 |
| RsaI | GTAC | 1 cut(s) | 2279 |
| RsaNI | GTAC | 1 cut(s) | 2278 |
| RseI | CAYNNNNRTG | 2 cut(s) | 717, 1905 |
| SacI | GAGCTC | 1 cut(s) | 9 |
| SaqAI | TTAA | 3 cut(s) | 585, 1275, 2058 |
| Sau3AI | GATC | 9 cut(s) | 126, 192, 1089, 1117, 1490, 1787, 1850, 2161, 2371 |
| Sau96I | GGNCC | 5 cut(s) | 536, 778, 1052, 1583, 1820 |
| SchI | GAGTC | 4 cut(s) | 363, 515, 1232, 2126 |
| ScrFI | CCNGG | 6 cut(s) | 1015, 1166, 1558, 1632, 1922, 1923 |
| SduI | GDGCHC | 4 cut(s) | 9, 625, 811, 1902 |
| SfaNI | GCATC | 3 cut(s) | 697, 1669, 1768 |
| SfcI | CTRYAG | 2 cut(s) | 741, 1248 |
| SinI | GGWCC | 3 cut(s) | 1052, 1583, 1820 |
| SmaI | CCCGGG | 1 cut(s) | 1923 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 717, 1905 |
| SmlI | CTYRAG | 3 cut(s) | 315, 1190, 1610 |
| SmoI | CTYRAG | 3 cut(s) | 315, 1190, 1610 |
| SpeI | ACTAGT | 1 cut(s) | 1795 |
| SphI | GCATGC | 1 cut(s) | 2318 |
| SseBI | AGGCCT | 1 cut(s) | 531 |
| SsiI | CCGC | 5 cut(s) | 61, 858, 1374, 1538, 1593 |
| SspI | AATATT | 2 cut(s) | 439, 1551 |
| SspMI | CTAG | 4 cut(s) | 390, 533, 735, 1796 |
| SstI | GAGCTC | 1 cut(s) | 9 |
| StuI | AGGCCT | 1 cut(s) | 531 |
| StyD4I | CCNGG | 6 cut(s) | 1013, 1164, 1556, 1630, 1920, 1921 |
| StyI | CCWWGG | 5 cut(s) | 532, 781, 1085, 1284, 1974 |
| TaiI | ACGT | 2 cut(s) | 628, 979 |
| TaqI | TCGA | 8 cut(s) | 352, 820, 935, 1120, 1709, 1786, 1878, 2238 |
| TaqII | GACCGA | 1 cut(s) | 1313 |
| TfiI | GAWTC | 5 cut(s) | 200, 569, 1262, 1399, 1667 |
| Tru1I | TTAA | 3 cut(s) | 585, 1275, 2058 |
| Tru9I | TTAA | 3 cut(s) | 585, 1275, 2058 |
| TscAI | CASTG | 6 cut(s) | 104, 557, 736, 745, 1668, 1761 |
| TseFI | GTSAC | 4 cut(s) | 757, 927, 1798, 2116 |
| Tsp45I | GTSAC | 4 cut(s) | 757, 927, 1798, 2116 |
| TspGWI | ACGGA | 5 cut(s) | 212, 323, 1130, 2131, 2319 |
| TspMI | CCCGGG | 1 cut(s) | 1921 |
| TspRI | CASTG | 6 cut(s) | 104, 557, 736, 745, 1668, 1761 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1409 |
| VneI | GTGCAC | 1 cut(s) | 1898 |
| VpaK11BI | GGWCC | 3 cut(s) | 1052, 1583, 1820 |
| XapI | RAATTY | 4 cut(s) | 46, 581, 689, 2325 |
| XceI | RCATGY | 3 cut(s) | 1960, 2028, 2318 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 454 |
| XmaI | CCCGGG | 1 cut(s) | 1921 |
| XmaJI | CCTAGG | 1 cut(s) | 532 |
| XmiI | GTMKAC | 1 cut(s) | 264 |
| XspI | CTAG | 4 cut(s) | 390, 533, 735, 1796 |
| Zsp2I | ATGCAT | 1 cut(s) | 1962 |