Prupe.5G133600_v2.0.a1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0001127
Physical Location & Seq
Forward (+)
12784169 .. 12787988
3820 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G133600.1

Sequence Viewer

Length: 2352 bp
ATGGCTTCTCTTTTATTGAAGAAGATGAGCAACAAAGTTTCAGTGTTTGTGACATTTAATATTTTCTTAGTGCTCTGTACCCAAAACACTATGATTAGGACGGTCCATGCCAATAGTAAAGTTCACATAGTCTATATGGGTGAAAAGCATCACCATGATCCTGAGGTTGTAACCAGTTTGCATCACGACATGCTGGCCTCAGTTTTAGGGAGCAAAGAAGCCGCTTATGATTCTATGGTGTACAGTTACAAGCATGGATTCTCCGGTTTCGCAGCTAAAGTCACAGAATCTCAAGCTCAGAAGATTGCAGAGTTACCTGGAGTTATCCGGGTTATGCCCAGTCACTTTTATTCTCTGCAAACAACTAGGAGTTGGGATTATCTTGGCCTCTCTCCTAGTTCTCCCACTAACCTTTTGCATGACACAAACTTGGGAGATGGAATTGTCATTGGCCTTCTGGACACAGGTATATGGCCAGAGTCAAAGGTATTCAATGATGAAGGGCTTGGACCAATCCCAAACCAATGGAAAGGTCAGTGTGTATCTGGAGAGAGTTTCAATGCCTCAGCAGATTGTAACAAAAAACTTATAGGAGCAAAATGGTACATTGATGGTTTCCTTGCTGAGAATAAGCAGCCATTCAACACCACTGACAGCCCAGATTTCTTGTCCCCAAGAGATGTTTTTGGACATGGAACACACACATCTACCATTGCTGGTGGCTCTTTTGTGTACAATGCCAGCTACAGAGGCCTTGGCCTGGGATCAGTAAGAGGTGGAGCGCCCCGCGCTCGCTTGGCGATGTATAAAGTGTGCTGGAATGTGCCAAGGGGGCAGTGCTCATCTGCTGACATATTGAAAGCTTTTGATGATGCAATTCATGATGGAGTTGATGTTATATCAGTATCTCTTGGCACCCAACTTCCTCTGTTTTCAGAGGTTGATGACCGCGATACGATTTCCATTGGTTCGTTCCATGCTGTGGCCAAGGGTATACCTGTTGTTTGTGGAGCTGCAAATGAGGGGCCTTCTGCATACACAGTTGAAAACACAGCACCATGGATCTTAACCGTAGCAGCTACTACAATTGATCGATCCTTTCCTACACCTATTACACTTGGGAACAACTTAACCATTCTGGGTCAAGCCATTTTTGCAGGAAAAGAAGTTGGTTTTACTGGTTTGGTATACCCAGAAAACCCAGGACTTATCCCTTCACTAGCTGGCGTCTGCGAGTCCCTTTTACTTAACAACACACCGGTGGCTGGAAATGTAGTTCTCTGCTTCACAACAGTGGCCAGTAGAACTCCAGTAGCAACTGCGGTCTCCAGTGTTAGGGCAGCTGGTGGGGTTGGGGTTATTGTTGCCAAATCTCCTGGTGATGTCTTAGGTCCATGTAGCAATGAGTTCCCATGCATTGAAGTAGACTATGAGCTTGGCACTCAGATACTATTCTACATCCGTTCCACCAGATCTCCAACCGTAAAATTGAGCCCTTCTGCAACACTGGTTGGGAAGCCTATATCAACCAAGGTTGCCACTTTTTCATCTAGAGGGCCTAACTCCATTGCTCCAGCCATACTCAAGCCAGACATAGCCGCTCCCGGTGTGAGCATATTAGCTGGCAGTTCTCCCTATGATTCTTTCATGGATGGAGGATTTGCCTTGCATTCAGGAACATCAATGGCAACTCCTCATGTCTCAGGCATTGTGGCACTTTTGAAAGCATTGCACTCCAATTGGTCCCCTGCTGCAATAAGATCGGCCCTAGTAACAACAGCATGGAAGACGGATCCATTCGGAGAGCCTATCTTCGCTGAGGGGTCACCCCAGAAGGTTGCTAATCCATTTGATTATGGAGGAGGCCTTGTGAACCCAAACAAGGCAGCAGACCCTGGTCTCATATATGACATGGGCACAGAAGACTACATTAAGTACCTTTGTGCTGTTGGCTACAACACCTCAGCCATCTCTCAACTTGTTGGACAAACCACAGCTTGTTCCATGGTCAAGCCTTCTGTTCTGGATGTGAACCTGCCTTCCATTACAATTCCAAATCTGAGAGAAAATATTACACTCACCAGAAGTGTCACAAATGTTGGTCCAGTCAACTCAGTATACAAAGCTAACATAGACCCTCCACCTGGCATCAGTGTAGCTGTTAGGCCTGAGACTTTGGTCTTCAACTCCACCATTAAAACAATCTCTTTCACTGTTGCAGTTTCCACCACCCACCAAGTGAACACAGGATACTACTTTGGAAGCCTCACTTGGACAGATGGGGAGCATTTGGTGACAAGTCCCATATCTGTTAGAACACAAATCATTCAGTATTATACAGATGGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

784

Amino Acids

83.11

Weight (kDa)

6.25

Isoelectric Point (pI)

33.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2043
AccB1I GGYRCC 1 cut(s) 914
AccB7I CCANNNNNTGG 1 cut(s) 982
AccBSI CCGCTC 1 cut(s) 1601
AccI GTMKAC 4 cut(s) 994, 1188, 1425, 2118
AccII CGCG 2 cut(s) 789, 951
AciI CCGC 5 cut(s) 222, 787, 949, 1322, 1599
AclWI GGATC 6 cut(s) 152, 772, 1070, 1089, 1787, 1800
AcoI YGGCCR 3 cut(s) 473, 984, 1296
AcyI GRCGYC 1 cut(s) 1227
AdeI CACNNNGTG 1 cut(s) 2239
AfaI GTAC 5 cut(s) 79, 242, 605, 734, 1937
AfiI CCNNNNNNNGG 6 cut(s) 760, 982, 1265, 1335, 1882, 2144
AgeI ACCGGT 1 cut(s) 1258
AgsI TTSAA 9 cut(s) 19, 493, 559, 643, 859, 1046, 1421, 1723, 2185
AjnI CCWGG 6 cut(s) 316, 759, 1201, 1375, 1894, 2143
AjuI GAANNNNNNNTTGG 2 cut(s) 2254, 2286
AleI CACNNNNGTG 2 cut(s) 1259, 1292
AloI GAACNNNNNNTCC 4 cut(s) 1260, 1292, 2024, 2056
Alw21I GWGCWC 2 cut(s) 75, 842
Alw26I GTCTC 4 cut(s) 1330, 1705, 1904, 2165
AlwI GGATC 6 cut(s) 152, 772, 1070, 1089, 1787, 1800
AlwNI CAGNNNCTG 1 cut(s) 1895
ApeKI GCWGC 7 cut(s) 272, 634, 1013, 1076, 1340, 1751, 1886
AsiGI ACCGGT 1 cut(s) 1258
AspLEI GCGC 2 cut(s) 784, 791
AspS9I GGNCC 8 cut(s) 103, 509, 1025, 1391, 1556, 1743, 1765, 2102
AsuC2I CCSGG 2 cut(s) 329, 1605
AsuHPI GGTGA 6 cut(s) 143, 152, 1391, 1818, 2071, 2305
AvaII GGWCC 5 cut(s) 103, 509, 1391, 1743, 2102
AxyI CCTNAGG 1 cut(s) 162
BaeGI GKGCMC 1 cut(s) 1919
BalI TGGCCA 3 cut(s) 475, 986, 1298
BamHI GGATCC 1 cut(s) 1792
BanI GGYRCC 1 cut(s) 914
BanII GRGCYC 1 cut(s) 1496
BbsI GAAGAC 3 cut(s) 1793, 1929, 2173
Bbv12I GWGCWC 2 cut(s) 75, 842
BbvCI CCTCAGC 3 cut(s) 565, 1818, 1963
BbvI GCAGC 7 cut(s) 284, 646, 1000, 1088, 1352, 1738, 1898
BccI CCATC 7 cut(s) 431, 605, 878, 1646, 1976, 2273, 2336
BcgI CGANNNNNNTGC 2 cut(s) 1743, 1777
BciT130I CCWGG 6 cut(s) 318, 761, 1203, 1377, 1896, 2145
BciVI GTATCC 1 cut(s) 2243
BcnI CCSGG 2 cut(s) 329, 1605
BcoDI GTCTC 4 cut(s) 1330, 1705, 1904, 2165
BfaI CTAG 5 cut(s) 366, 396, 1220, 1551, 1769
BfmI CTRYAG 1 cut(s) 745
BfoI RGCGCY 1 cut(s) 785
BfuAI ACCTGC 1 cut(s) 2043
BfuI GTATCC 1 cut(s) 2243
BglI GCCNNNNNGGC 1 cut(s) 832
BglII AGATCT 1 cut(s) 1472
BisI GCNGC 9 cut(s) 222, 273, 635, 1014, 1077, 1341, 1599, 1752, 1887
BlsI GCNGC 9 cut(s) 223, 274, 636, 1015, 1078, 1342, 1600, 1753, 1888
Bme1390I CCNGG 8 cut(s) 318, 329, 761, 1203, 1377, 1605, 1896, 2145
Bme18I GGWCC 5 cut(s) 103, 509, 1391, 1743, 2102
BmgT120I GGNCC 8 cut(s) 103, 509, 1025, 1391, 1556, 1743, 1765, 2102
BmiI GGNNCC 4 cut(s) 916, 1026, 1745, 1794
BmrFI CCNGG 8 cut(s) 318, 329, 761, 1203, 1377, 1605, 1896, 2145
BmrI ACTGGG 1 cut(s) 333
BmsI GCATC 4 cut(s) 157, 190, 862, 2157
BmuI ACTGGG 1 cut(s) 333
BoxI GACNNNNGTC 2 cut(s) 1896, 2177
BpiI GAAGAC 3 cut(s) 1793, 1929, 2173
BpmI CTGGAG 5 cut(s) 339, 567, 1293, 1312, 1557
Bpu10I CCTNAGC 3 cut(s) 565, 1818, 1963
BpuEI CTTGAG 2 cut(s) 276, 1568
BpuMI CCSGG 2 cut(s) 329, 1605
Bsa29I ATCGAT 1 cut(s) 1093
BsaHI GRCGYC 1 cut(s) 1227
BsaI GGTCTC 2 cut(s) 1330, 1904
BsaJI CCNNGG 9 cut(s) 754, 760, 827, 987, 1058, 1201, 1530, 1894, 2004
BsaWI WCCGGW 2 cut(s) 263, 1258
BsaXI ACNNNNNCTCC 6 cut(s) 879, 909, 1459, 1489, 1854, 1884
Bsc4I CCNNNNNNNGG 6 cut(s) 760, 982, 1265, 1335, 1882, 2144
Bse118I RCCGGY 1 cut(s) 1258
Bse1I ACTGG 8 cut(s) 174, 339, 1183, 1299, 1310, 1329, 1512, 2105
Bse21I CCTNAGG 1 cut(s) 162
Bse3DI GCAATG 4 cut(s) 711, 1408, 1566, 1727
BseBI CCWGG 6 cut(s) 318, 761, 1203, 1377, 1896, 2145
BseCI ATCGAT 1 cut(s) 1093
BseDI CCNNGG 9 cut(s) 754, 760, 827, 987, 1058, 1201, 1530, 1894, 2004
BseGI GGATG 3 cut(s) 1458, 1657, 2032
BseLI CCNNNNNNNGG 6 cut(s) 760, 982, 1265, 1335, 1882, 2144
BseMI GCAATG 4 cut(s) 711, 1408, 1566, 1727
BseNI ACTGG 8 cut(s) 174, 339, 1183, 1299, 1310, 1329, 1512, 2105
BseRI GAGGAG 2 cut(s) 1683, 1875
BseSI GKGCMC 1 cut(s) 1919
BseXI GCAGC 7 cut(s) 284, 646, 1000, 1088, 1352, 1738, 1898
Bsh1236I CGCG 2 cut(s) 789, 951
BshNI GGYRCC 1 cut(s) 914
BshTI ACCGGT 1 cut(s) 1258
BshVI ATCGAT 1 cut(s) 1093
BsiHKAI GWGCWC 2 cut(s) 75, 842
BsiSI CCGG 4 cut(s) 264, 328, 1259, 1605
BslFI GGGAC 4 cut(s) 655, 1222, 1729, 2286
BslI CCNNNNNNNGG 6 cut(s) 760, 982, 1265, 1335, 1882, 2144
BsmAI GTCTC 4 cut(s) 1330, 1705, 1904, 2165
BsmFI GGGAC 4 cut(s) 655, 1222, 1729, 2286
BsmI GAATGC 1 cut(s) 1669
Bso31I GGTCTC 2 cut(s) 1330, 1904
Bsp1286I GDGCHC 4 cut(s) 75, 842, 1496, 1919
Bsp1407I TGTACA 2 cut(s) 240, 732
Bsp143I GATC 8 cut(s) 157, 764, 1062, 1090, 1094, 1472, 1760, 1792
Bsp19I CCATGG 2 cut(s) 1058, 2004
BspACI CCGC 5 cut(s) 222, 787, 949, 1322, 1599
BspDI ATCGAT 1 cut(s) 1093
BspFNI CGCG 2 cut(s) 789, 951
BspHI TCATGA 1 cut(s) 880
BspLI GGNNCC 4 cut(s) 916, 1026, 1745, 1794
BspMI ACCTGC 1 cut(s) 2043
BspPI GGATC 6 cut(s) 152, 772, 1070, 1089, 1787, 1800
BspT107I GGYRCC 1 cut(s) 914
BspTNI GGTCTC 2 cut(s) 1330, 1904
BsrBI CCGCTC 1 cut(s) 1601
BsrDI GCAATG 4 cut(s) 711, 1408, 1566, 1727
BsrFI RCCGGY 1 cut(s) 1258
BsrGI TGTACA 2 cut(s) 240, 732
BsrI ACTGG 8 cut(s) 174, 339, 1183, 1299, 1310, 1329, 1512, 2105
BssAI RCCGGY 1 cut(s) 1258
BssECI CCNNGG 9 cut(s) 754, 760, 827, 987, 1058, 1201, 1530, 1894, 2004
BssMI GATC 8 cut(s) 157, 764, 1062, 1090, 1094, 1472, 1760, 1792
BssNAI GTATAC 3 cut(s) 995, 1189, 2119
BssNI GRCGYC 1 cut(s) 1227
BssT1I CCWWGG 6 cut(s) 754, 827, 987, 1058, 1530, 2004
Bst1107I GTATAC 3 cut(s) 995, 1189, 2119
Bst2UI CCWGG 6 cut(s) 318, 761, 1203, 1377, 1896, 2145
Bst4CI ACNGT 7 cut(s) 103, 245, 1042, 1072, 1294, 1483, 2215
BstACI GRCGYC 1 cut(s) 1227
BstAUI TGTACA 2 cut(s) 240, 732
BstC8I GCNNGC 5 cut(s) 195, 742, 793, 1225, 1624
BstDSI CCRYGG 2 cut(s) 1058, 2004
BstEII GGTNACC 1 cut(s) 1824
BstF5I GGATG 3 cut(s) 1458, 1657, 2032
BstFNI CGCG 2 cut(s) 789, 951
BstH2I RGCGCY 1 cut(s) 785
BstHHI GCGC 2 cut(s) 784, 791
BstKTI GATC 8 cut(s) 160, 767, 1065, 1093, 1097, 1475, 1763, 1795
BstMAI GTCTC 4 cut(s) 1330, 1705, 1904, 2165
BstMBI GATC 8 cut(s) 157, 764, 1062, 1090, 1094, 1472, 1760, 1792
BstMWI GCNNNNNNNGC 5 cut(s) 750, 788, 797, 832, 1154
BstNI CCWGG 6 cut(s) 318, 761, 1203, 1377, 1896, 2145
BstNSI RCATGY 1 cut(s) 193
BstPAI GACNNNNGTC 2 cut(s) 1896, 2177
BstPI GGTNACC 1 cut(s) 1824
BstSCI CCNGG 8 cut(s) 316, 327, 759, 1201, 1375, 1603, 1894, 2143
BstSFI CTRYAG 1 cut(s) 745
BstSLI GKGCMC 1 cut(s) 1919
BstUI CGCG 2 cut(s) 789, 951
BstV1I GCAGC 7 cut(s) 284, 646, 1000, 1088, 1352, 1738, 1898
BstV2I GAAGAC 3 cut(s) 1793, 1929, 2173
BstX2I RGATCY 3 cut(s) 1062, 1472, 1792
BstXI CCANNNNNNTGG 1 cut(s) 525
BstYI RGATCY 3 cut(s) 1062, 1472, 1792
BstZ17I GTATAC 3 cut(s) 995, 1189, 2119
Bsu15I ATCGAT 1 cut(s) 1093
Bsu36I CCTNAGG 1 cut(s) 162
BsuI GTATCC 1 cut(s) 2243
BsuTUI ATCGAT 1 cut(s) 1093
BtgI CCRYGG 2 cut(s) 1058, 2004
BtgZI GCGATG 1 cut(s) 815
BtsCI GGATG 3 cut(s) 1458, 1657, 2032
BtsI GCAGTG 1 cut(s) 842
BtsIMutI CAGTG 9 cut(s) 48, 542, 648, 842, 1299, 1336, 1505, 2158, 2211
BveI ACCTGC 1 cut(s) 2043
Cac8I GCNNGC 5 cut(s) 195, 742, 793, 1225, 1624
CaiI CAGNNNCTG 1 cut(s) 1895
CciI TCATGA 1 cut(s) 880
CfoI GCGC 2 cut(s) 784, 791
Cfr10I RCCGGY 1 cut(s) 1258
Cfr13I GGNCC 8 cut(s) 103, 509, 1025, 1391, 1556, 1743, 1765, 2102
ClaI ATCGAT 1 cut(s) 1093
CseI GACGC 1 cut(s) 1216
Csp6I GTAC 5 cut(s) 78, 241, 604, 733, 1936
CspAI ACCGGT 1 cut(s) 1258
CviQI GTAC 5 cut(s) 78, 241, 604, 733, 1936
DpnI GATC 8 cut(s) 159, 766, 1064, 1092, 1096, 1474, 1762, 1794
DpnII GATC 8 cut(s) 157, 764, 1062, 1090, 1094, 1472, 1760, 1792
DraIII CACNNNGTG 1 cut(s) 2239
EaeI YGGCCR 3 cut(s) 473, 984, 1296
Eco130I CCWWGG 6 cut(s) 754, 827, 987, 1058, 1530, 2004
Eco147I AGGCCT 3 cut(s) 753, 1866, 2167
Eco24I GRGCYC 1 cut(s) 1496
Eco31I GGTCTC 2 cut(s) 1330, 1904
Eco47I GGWCC 5 cut(s) 103, 509, 1391, 1743, 2102
Eco81I CCTNAGG 1 cut(s) 162
Eco91I GGTNACC 1 cut(s) 1824
EcoO109I RGGNCCY 2 cut(s) 1025, 1556
EcoO65I GGTNACC 1 cut(s) 1824
EcoRII CCWGG 6 cut(s) 316, 759, 1201, 1375, 1894, 2143
EcoT14I CCWWGG 6 cut(s) 754, 827, 987, 1058, 1530, 2004
EcoT22I ATGCAT 1 cut(s) 1418
EcoT38I GRGCYC 1 cut(s) 1496
ErhI CCWWGG 6 cut(s) 754, 827, 987, 1058, 1530, 2004
FalI AAGNNNNNCTT 2 cut(s) 2254, 2286
FaqI GGGAC 4 cut(s) 655, 1222, 1729, 2286
FauI CCCGC 1 cut(s) 794
FblI GTMKAC 4 cut(s) 994, 1188, 1425, 2118
Fnu4HI GCNGC 9 cut(s) 222, 273, 635, 1014, 1077, 1341, 1599, 1752, 1887
FokI GGATG 3 cut(s) 1445, 1664, 2039
FriOI GRGCYC 1 cut(s) 1496
Fsp4HI GCNGC 9 cut(s) 222, 273, 635, 1014, 1077, 1341, 1599, 1752, 1887
FspBI CTAG 5 cut(s) 366, 396, 1220, 1551, 1769
GlaI GCGC 2 cut(s) 783, 790
GluI GCNGC 9 cut(s) 222, 273, 635, 1014, 1077, 1341, 1599, 1752, 1887
GsuI CTGGAG 5 cut(s) 339, 567, 1293, 1312, 1557
HaeII RGCGCY 1 cut(s) 785
HapII CCGG 4 cut(s) 264, 328, 1259, 1605
HgaI GACGC 1 cut(s) 1216
HhaI GCGC 2 cut(s) 784, 791
Hin1I GRCGYC 1 cut(s) 1227
Hin6I GCGC 2 cut(s) 782, 789
HinP1I GCGC 2 cut(s) 782, 789
HincII GTYRAC 1 cut(s) 2110
HindII GTYRAC 1 cut(s) 2110
HindIII AAGCTT 1 cut(s) 861
HinfI GANTC 6 cut(s) 230, 258, 287, 479, 1235, 1640
HpaII CCGG 4 cut(s) 264, 328, 1259, 1605
HphI GGTGA 6 cut(s) 143, 152, 1391, 1818, 2071, 2305
Hpy188I TCNGA 5 cut(s) 300, 937, 1446, 1802, 2061
Hpy188III TCNNGA 8 cut(s) 161, 185, 458, 546, 881, 1551, 1674, 2024
HpyAV CCTTC 8 cut(s) 464, 494, 1038, 1224, 1506, 1828, 2025, 2049
HpyCH4III ACNGT 7 cut(s) 103, 245, 1042, 1072, 1294, 1483, 2215
HpyF10VI GCNNNNNNNGC 5 cut(s) 750, 788, 797, 832, 1154
Hsp92I GRCGYC 1 cut(s) 1227
HspAI GCGC 2 cut(s) 782, 789
Kzo9I GATC 8 cut(s) 157, 764, 1062, 1090, 1094, 1472, 1760, 1792
LmnI GCTCC 7 cut(s) 210, 593, 779, 1010, 1576, 1606, 2284
Lsp1109I GCAGC 7 cut(s) 284, 646, 1000, 1088, 1352, 1738, 1898
LweI GCATC 4 cut(s) 157, 190, 862, 2157
MaeI CTAG 5 cut(s) 366, 396, 1220, 1551, 1769
MalI GATC 8 cut(s) 159, 766, 1064, 1092, 1096, 1474, 1762, 1794
MbiI CCGCTC 1 cut(s) 1601
MboI GATC 8 cut(s) 157, 764, 1062, 1090, 1094, 1472, 1760, 1792
MboII GAAGA 7 cut(s) 31, 34, 313, 1798, 1804, 1934, 2173
MfeI CAATTG 3 cut(s) 1086, 1738, 2347
MflI RGATCY 3 cut(s) 1062, 1472, 1792
MhlI GDGCHC 4 cut(s) 75, 842, 1496, 1919
MlsI TGGCCA 3 cut(s) 475, 986, 1298
MluCI AATT 7 cut(s) 441, 876, 1086, 1487, 1738, 2049, 2347
MluNI TGGCCA 3 cut(s) 475, 986, 1298
MlyI GAGTC 2 cut(s) 488, 1244
MmeI TCCRAC 2 cut(s) 1502, 1963
Mox20I TGGCCA 3 cut(s) 475, 986, 1298
Mph1103I ATGCAT 1 cut(s) 1418
MscI TGGCCA 3 cut(s) 475, 986, 1298
MseI TTAA 6 cut(s) 57, 1067, 1130, 1248, 1932, 2196
MslI CAYNNNNRTG 3 cut(s) 153, 1259, 1292
Msp20I TGGCCA 3 cut(s) 475, 986, 1298
MspA1I CMGCKG 1 cut(s) 1343
MspI CCGG 4 cut(s) 264, 328, 1259, 1605
MspR9I CCNGG 8 cut(s) 318, 329, 761, 1203, 1377, 1605, 1896, 2145
MunI CAATTG 3 cut(s) 1086, 1738, 2347
Mva1269I GAATGC 1 cut(s) 1669
MvaI CCWGG 6 cut(s) 318, 761, 1203, 1377, 1896, 2145
MvnI CGCG 2 cut(s) 789, 951
MwoI GCNNNNNNNGC 5 cut(s) 750, 788, 797, 832, 1154
NciI CCSGG 2 cut(s) 329, 1605
NcoI CCATGG 2 cut(s) 1058, 2004
NdeII GATC 8 cut(s) 157, 764, 1062, 1090, 1094, 1472, 1760, 1792
NlaIV GGNNCC 4 cut(s) 916, 1026, 1745, 1794
NmuCI GTSAC 6 cut(s) 49, 280, 341, 1824, 2089, 2293
NsiI ATGCAT 1 cut(s) 1418
NspI RCATGY 1 cut(s) 193
OliI CACNNNNGTG 2 cut(s) 1259, 1292
PagI TCATGA 1 cut(s) 880
PceI AGGCCT 3 cut(s) 753, 1866, 2167
PctI GAATGC 1 cut(s) 1669
PfeI GAWTC 4 cut(s) 230, 258, 287, 1640
PflMI CCANNNNNTGG 1 cut(s) 982
PinAI ACCGGT 1 cut(s) 1258
PkrI GCNGC 9 cut(s) 223, 274, 636, 1015, 1078, 1342, 1600, 1753, 1888
PleI GAGTC 2 cut(s) 487, 1243
PpsI GAGTC 2 cut(s) 487, 1243
PshAI GACNNNNGTC 2 cut(s) 1896, 2177
Psp6I CCWGG 6 cut(s) 316, 759, 1201, 1375, 1894, 2143
PspEI GGTNACC 1 cut(s) 1824
PspGI CCWGG 6 cut(s) 316, 759, 1201, 1375, 1894, 2143
PspN4I GGNNCC 4 cut(s) 916, 1026, 1745, 1794
PspPI GGNCC 8 cut(s) 103, 509, 1025, 1391, 1556, 1743, 1765, 2102
PsrI GAACNNNNNNTAC 2 cut(s) 2234, 2266
PstNI CAGNNNCTG 1 cut(s) 1895
PsuI RGATCY 3 cut(s) 1062, 1472, 1792
PvuII CAGCTG 1 cut(s) 1343
RsaI GTAC 5 cut(s) 79, 242, 605, 734, 1937
RsaNI GTAC 5 cut(s) 78, 241, 604, 733, 1936
RseI CAYNNNNRTG 3 cut(s) 153, 1259, 1292
SaqAI TTAA 6 cut(s) 57, 1067, 1130, 1248, 1932, 2196
SatI GCNGC 9 cut(s) 222, 273, 635, 1014, 1077, 1341, 1599, 1752, 1887
Sau3AI GATC 8 cut(s) 157, 764, 1062, 1090, 1094, 1472, 1760, 1792
Sau96I GGNCC 8 cut(s) 103, 509, 1025, 1391, 1556, 1743, 1765, 2102
SchI GAGTC 2 cut(s) 488, 1244
ScrFI CCNGG 8 cut(s) 318, 329, 761, 1203, 1377, 1605, 1896, 2145
SduI GDGCHC 4 cut(s) 75, 842, 1496, 1919
SfaNI GCATC 4 cut(s) 157, 190, 862, 2157
SfcI CTRYAG 1 cut(s) 745
SgrAI CRCCGGYG 1 cut(s) 1258
SinI GGWCC 5 cut(s) 103, 509, 1391, 1743, 2102
SmiMI CAYNNNNRTG 3 cut(s) 153, 1259, 1292
SmlI CTYRAG 2 cut(s) 291, 1583
SmoI CTYRAG 2 cut(s) 291, 1583
Sse9I AATT 7 cut(s) 441, 876, 1086, 1487, 1738, 2049, 2347
SseBI AGGCCT 3 cut(s) 753, 1866, 2167
SsiI CCGC 5 cut(s) 222, 787, 949, 1322, 1599
SspI AATATT 2 cut(s) 61, 2071
SspMI CTAG 5 cut(s) 366, 396, 1220, 1551, 1769
StuI AGGCCT 3 cut(s) 753, 1866, 2167
StyD4I CCNGG 8 cut(s) 316, 327, 759, 1201, 1375, 1603, 1894, 2143
StyI CCWWGG 6 cut(s) 754, 827, 987, 1058, 1530, 2004
TaaI ACNGT 7 cut(s) 103, 245, 1042, 1072, 1294, 1483, 2215
TaqI TCGA 1 cut(s) 1093
TasI AATT 7 cut(s) 441, 876, 1086, 1487, 1738, 2049, 2347
TatI WGTACW 2 cut(s) 240, 732
TauI GCSGC 2 cut(s) 224, 1601
TfiI GAWTC 4 cut(s) 230, 258, 287, 1640
Tru1I TTAA 6 cut(s) 57, 1067, 1130, 1248, 1932, 2196
Tru9I TTAA 6 cut(s) 57, 1067, 1130, 1248, 1932, 2196
TscAI CASTG 9 cut(s) 48, 542, 655, 842, 1299, 1336, 1512, 2158, 2218
TseFI GTSAC 6 cut(s) 49, 280, 341, 1824, 2089, 2293
TseI GCWGC 7 cut(s) 272, 634, 1013, 1076, 1340, 1751, 1886
Tsp45I GTSAC 6 cut(s) 49, 280, 341, 1824, 2089, 2293
TspDTI ATGAA 4 cut(s) 513, 869, 1536, 1636
TspGWI ACGGA 2 cut(s) 1451, 1805
TspRI CASTG 9 cut(s) 48, 542, 655, 842, 1299, 1336, 1512, 2158, 2218
Van91I CCANNNNNTGG 1 cut(s) 982
VpaK11BI GGWCC 5 cut(s) 103, 509, 1391, 1743, 2102
XbaI TCTAGA 1 cut(s) 1550
XceI RCATGY 1 cut(s) 193
XmiI GTMKAC 4 cut(s) 994, 1188, 1425, 2118
XspI CTAG 5 cut(s) 366, 396, 1220, 1551, 1769
Zsp2I ATGCAT 1 cut(s) 1418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.