Rh7AG088500

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001127
Physical Location & Seq
Forward (+)
6735043 .. 6737755
2713 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG088500.1

Sequence Viewer

Length: 1104 bp
ATGGGATCTCATCTAATGAAGAAGAGCAACAAAGTTCCACTGTTTGTGACATTCAATCTTTTCTTAGTCCTCTGTAGCCAAACCATCATAATTAGGACGGTGGACGCCAAGAGTAAAGTTCACATAGTCTATATGGGTAAAAGGCACCACCATGATCCTGAGGTTGTAACCTGTTTGCATCACGACATGCTTGCCTCTGTTGTGGGGAGCAAAGAAGCAGCAACTGATGCTATGGTGTACAGTTACAAACATGGATTTTCTGGTTTTGCAGCTAAATTTACAGAATCTCAAGCTAAGAAGATGGCAGGCATACCTGTTGTTGGTGGAGCTGCAAATGAAGGATCTTCTGCATACACTGTCGAAAACACAGCACCTTGGATCTTAACTGTAGCAGCTTCTACTATAGATCGGTCTTTTCCTACAAATATCACACTAGGGAACAACTCAACCATACTGGGTCAAGCCCTGTTTGCAGGAACGGAAGGTGACTTTACTGGTTTGGTGTACCCGGAAAACCCAGGACTTATTCCTTCACTAGCCGGCGTCTGTGAGGCTCTTATACTCAATAACACACCTGTGGCTGGAAATGTAGTCCTTTGCTTTACATCAGTGGCCAGAAGAACTCCGGTAGCACTTGCTGTATCTAGTGTCAGAGCAGCTGGTGGGGTTGGAGTAATTGTTGACAAGAGTCCTGGTGATGTCTTGGGTCCATGTAGCAGTGACTTTCCATGCATTGAAGTAGAATACGAGCTTGGTACTCAGATACTATTCTACATTCGCTCCACCAGTTCTCCTGTAGTAAAATTGAGTCCTTCTATGACACTGGTGGGGAAGCCTGTATCGACCAAGGTTGCCGCTTTCTCATCTAGAGGGCCTAACTCCATTTCTCCAGCCATACTACAGCCGGACATAGCTGCACCTGGTGTGAGCATATTAGCTGCCAGTTCTCCTTTTGATCTGTATATGAATGGAGGATTTGCCCTGCATTCAGGAACATCAATGGCAACTCCTCATGTTTCGGGGATTGTGGCACTTCTAAAAGCATTGCACTCCGATTGGTCCCCTGCTGCCATAAGATCAGCAATAACAACAACAGGTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

367

Amino Acids

38.24

Weight (kDa)

8.13

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 40 - 107 1.3e-14 Peptidase inhibitor I9
Peptidase_S8 PF00082 98 - 365 5.4e-21 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 144
AciI CCGC 1 cut(s) 855
AclWI GGATC 4 cut(s) 13, 149, 349, 386
AcoI YGGCCR 1 cut(s) 612
AcsI RAATTY 1 cut(s) 275
AcyI GRCGYC 2 cut(s) 105, 543
AdeI CACNNNGTG 1 cut(s) 923
AfaI GTAC 3 cut(s) 239, 506, 757
AfiI CCNNNNNNNGG 2 cut(s) 320, 581
AgsI TTSAA 2 cut(s) 55, 737
AjnI CCWGG 3 cut(s) 517, 691, 919
AjuI GAANNNNNNNTTGG 2 cut(s) 735, 767
AleI CACNNNNGTG 1 cut(s) 575
AluBI AGCT 8 cut(s) 272, 293, 329, 395, 659, 751, 914, 938
AluI AGCT 8 cut(s) 272, 293, 329, 395, 659, 751, 914, 938
AlwI GGATC 4 cut(s) 13, 149, 349, 386
AlwNI CAGNNNCTG 1 cut(s) 224
AoxI GGCC 2 cut(s) 612, 872
ApeKI GCWGC 8 cut(s) 218, 269, 329, 392, 656, 914, 938, 1067
ApoI RAATTY 1 cut(s) 275
AspS9I GGNCC 3 cut(s) 707, 872, 1059
AsuC2I CCSGG 1 cut(s) 509
AsuHPI GGTGA 2 cut(s) 497, 707
AvaII GGWCC 2 cut(s) 707, 1059
AxyI CCTNAGG 1 cut(s) 159
BalI TGGCCA 1 cut(s) 614
BanI GGYRCC 1 cut(s) 144
BbvI GCAGC 8 cut(s) 230, 281, 316, 404, 668, 901, 925, 1054
BccI CCATC 2 cut(s) 92, 295
BcgI CGANNNNNNTGC 2 cut(s) 173, 207
BciT130I CCWGG 3 cut(s) 519, 693, 921
BcnI CCSGG 1 cut(s) 509
BfaI CTAG 4 cut(s) 434, 536, 645, 867
BfmI CTRYAG 5 cut(s) 73, 387, 402, 795, 899
BisI GCNGC 9 cut(s) 219, 270, 330, 393, 657, 855, 915, 939, 1068
BlsI GCNGC 9 cut(s) 220, 271, 331, 394, 658, 856, 916, 940, 1069
Bme1390I CCNGG 4 cut(s) 509, 519, 693, 921
Bme18I GGWCC 2 cut(s) 707, 1059
BmgT120I GGNCC 3 cut(s) 707, 872, 1059
BmiI GGNNCC 3 cut(s) 146, 708, 1061
BmrFI CCNGG 4 cut(s) 509, 519, 693, 921
BmrI ACTGGG 1 cut(s) 464
BmsI GCATC 2 cut(s) 187, 217
BmuI ACTGGG 1 cut(s) 464
BoxI GACNNNNGTC 1 cut(s) 687
BpmI CTGGAG 1 cut(s) 873
BpuEI CTTGAG 1 cut(s) 273
BpuMI CCSGG 1 cut(s) 509
BsaHI GRCGYC 2 cut(s) 105, 543
BsaJI CCNNGG 3 cut(s) 374, 517, 846
BsaWI WCCGGW 1 cut(s) 625
BsaXI ACNNNNNCTCC 6 cut(s) 663, 693, 764, 775, 794, 805
Bsc4I CCNNNNNNNGG 2 cut(s) 320, 581
Bse118I RCCGGY 1 cut(s) 539
Bse1I ACTGG 5 cut(s) 459, 499, 786, 828, 942
Bse21I CCTNAGG 1 cut(s) 159
Bse3DI GCAATG 1 cut(s) 1043
BseBI CCWGG 3 cut(s) 519, 693, 921
BseDI CCNNGG 3 cut(s) 374, 517, 846
BseLI CCNNNNNNNGG 2 cut(s) 320, 581
BseMI GCAATG 1 cut(s) 1043
BseMII CTCAG 2 cut(s) 150, 773
BseNI ACTGG 5 cut(s) 459, 499, 786, 828, 942
BseRI GAGGAG 1 cut(s) 999
BseXI GCAGC 8 cut(s) 230, 281, 316, 404, 668, 901, 925, 1054
BsgI GTGCAG 1 cut(s) 900
BshFI GGCC 2 cut(s) 614, 874
BshNI GGYRCC 1 cut(s) 144
BsiSI CCGG 4 cut(s) 509, 540, 626, 905
BslFI GGGAC 1 cut(s) 1045
BslI CCNNNNNNNGG 2 cut(s) 320, 581
BsmFI GGGAC 1 cut(s) 1045
BsmI GAATGC 1 cut(s) 985
BsnI GGCC 2 cut(s) 614, 874
Bsp1407I TGTACA 1 cut(s) 237
Bsp143I GATC 7 cut(s) 5, 154, 341, 378, 406, 955, 1076
BspACI CCGC 1 cut(s) 855
BspANI GGCC 2 cut(s) 614, 874
BspCNI CTCAG 2 cut(s) 151, 772
BspLI GGNNCC 3 cut(s) 146, 708, 1061
BspPI GGATC 4 cut(s) 13, 149, 349, 386
BspQI GCTCTTC 1 cut(s) 17
BspT107I GGYRCC 1 cut(s) 144
BsrDI GCAATG 1 cut(s) 1043
BsrFI RCCGGY 1 cut(s) 539
BsrGI TGTACA 1 cut(s) 237
BsrI ACTGG 5 cut(s) 459, 499, 786, 828, 942
BssAI RCCGGY 1 cut(s) 539
BssECI CCNNGG 3 cut(s) 374, 517, 846
BssMI GATC 7 cut(s) 5, 154, 341, 378, 406, 955, 1076
BssNI GRCGYC 2 cut(s) 105, 543
BssT1I CCWWGG 2 cut(s) 374, 846
Bst2UI CCWGG 3 cut(s) 519, 693, 921
Bst4CI ACNGT 5 cut(s) 42, 100, 242, 358, 388
Bst6I CTCTTC 1 cut(s) 17
BstACI GRCGYC 2 cut(s) 105, 543
BstAPI GCANNNNNTGC 1 cut(s) 227
BstAUI TGTACA 1 cut(s) 237
BstC8I GCNNGC 3 cut(s) 192, 307, 541
BstDEI CTNAG 4 cut(s) 64, 159, 294, 759
BstKTI GATC 7 cut(s) 8, 157, 344, 381, 409, 958, 1079
BstMBI GATC 7 cut(s) 5, 154, 341, 378, 406, 955, 1076
BstMWI GCNNNNNNNGC 2 cut(s) 227, 470
BstNI CCWGG 3 cut(s) 519, 693, 921
BstNSI RCATGY 1 cut(s) 190
BstPAI GACNNNNGTC 1 cut(s) 687
BstSCI CCNGG 4 cut(s) 507, 517, 691, 919
BstSFI CTRYAG 5 cut(s) 73, 387, 402, 795, 899
BstV1I GCAGC 8 cut(s) 230, 281, 316, 404, 668, 901, 925, 1054
BstX2I RGATCY 3 cut(s) 5, 341, 378
BstYI RGATCY 3 cut(s) 5, 341, 378
Bsu36I CCTNAGG 1 cut(s) 159
BsuRI GGCC 2 cut(s) 614, 874
BtsI GCAGTG 1 cut(s) 724
BtsIMutI CAGTG 5 cut(s) 38, 354, 615, 724, 821
Cac8I GCNNGC 3 cut(s) 192, 307, 541
CaiI CAGNNNCTG 1 cut(s) 224
Cfr10I RCCGGY 1 cut(s) 539
Cfr13I GGNCC 3 cut(s) 707, 872, 1059
CseI GACGC 2 cut(s) 113, 532
CsiI ACCWGGT 1 cut(s) 919
Csp6I GTAC 3 cut(s) 238, 505, 756
CviAII CATG 6 cut(s) 152, 187, 251, 711, 729, 1013
CviQI GTAC 3 cut(s) 238, 505, 756
DdeI CTNAG 4 cut(s) 64, 159, 294, 759
DpnI GATC 7 cut(s) 7, 156, 343, 380, 408, 957, 1078
DpnII GATC 7 cut(s) 5, 154, 341, 378, 406, 955, 1076
DraIII CACNNNGTG 1 cut(s) 923
EaeI YGGCCR 1 cut(s) 612
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
Eco130I CCWWGG 2 cut(s) 374, 846
Eco47I GGWCC 2 cut(s) 707, 1059
Eco81I CCTNAGG 1 cut(s) 159
EcoO109I RGGNCCY 1 cut(s) 872
EcoRII CCWGG 3 cut(s) 517, 691, 919
EcoT14I CCWWGG 2 cut(s) 374, 846
EcoT22I ATGCAT 1 cut(s) 734
ErhI CCWWGG 2 cut(s) 374, 846
FaeI CATG 6 cut(s) 155, 190, 254, 714, 732, 1016
FaqI GGGAC 1 cut(s) 1045
FatI CATG 6 cut(s) 151, 186, 250, 710, 728, 1012
Fnu4HI GCNGC 9 cut(s) 219, 270, 330, 393, 657, 855, 915, 939, 1068
Fsp4HI GCNGC 9 cut(s) 219, 270, 330, 393, 657, 855, 915, 939, 1068
FspBI CTAG 4 cut(s) 434, 536, 645, 867
GluI GCNGC 9 cut(s) 219, 270, 330, 393, 657, 855, 915, 939, 1068
GsuI CTGGAG 1 cut(s) 873
HaeIII GGCC 2 cut(s) 614, 874
HapII CCGG 4 cut(s) 509, 540, 626, 905
HgaI GACGC 2 cut(s) 113, 532
Hin1I GRCGYC 2 cut(s) 105, 543
Hin1II CATG 6 cut(s) 155, 190, 254, 714, 732, 1016
HincII GTYRAC 1 cut(s) 682
HindII GTYRAC 1 cut(s) 682
HinfI GANTC 3 cut(s) 284, 688, 808
HpaII CCGG 4 cut(s) 509, 540, 626, 905
HphI GGTGA 2 cut(s) 497, 707
Hpy166II GTNNAC 5 cut(s) 103, 121, 238, 505, 682
Hpy188I TCNGA 3 cut(s) 653, 762, 1054
Hpy188III TCNNGA 4 cut(s) 158, 182, 867, 990
Hpy8I GTNNAC 5 cut(s) 103, 121, 238, 505, 682
HpyAV CCTTC 4 cut(s) 332, 476, 540, 822
HpyCH4III ACNGT 5 cut(s) 42, 100, 242, 358, 388
HpyCH4V TGCA 9 cut(s) 178, 269, 332, 350, 473, 732, 917, 985, 1048
HpyF10VI GCNNNNNNNGC 2 cut(s) 227, 470
HpyF3I CTNAG 4 cut(s) 64, 159, 294, 759
Hsp92I GRCGYC 2 cut(s) 105, 543
Hsp92II CATG 6 cut(s) 155, 190, 254, 714, 732, 1016
KroI GCCGGC 1 cut(s) 539
KroNI GCCGGC 1 cut(s) 541
Kzo9I GATC 7 cut(s) 5, 154, 341, 378, 406, 955, 1076
LguI GCTCTTC 1 cut(s) 17
LmnI GCTCC 3 cut(s) 207, 326, 785
Lsp1109I GCAGC 8 cut(s) 230, 281, 316, 404, 668, 901, 925, 1054
LweI GCATC 2 cut(s) 187, 217
MabI ACCWGGT 1 cut(s) 919
MaeI CTAG 4 cut(s) 434, 536, 645, 867
MaeIII GTNAC 5 cut(s) 46, 166, 242, 485, 719
MalI GATC 7 cut(s) 7, 156, 343, 380, 408, 957, 1078
MboI GATC 7 cut(s) 5, 154, 341, 378, 406, 955, 1076
MboII GAAGA 5 cut(s) 31, 34, 310, 336, 630
MflI RGATCY 3 cut(s) 5, 341, 378
MlsI TGGCCA 1 cut(s) 614
MluCI AATT 4 cut(s) 90, 275, 675, 803
MluNI TGGCCA 1 cut(s) 614
MlyI GAGTC 2 cut(s) 697, 817
MmeI TCCRAC 1 cut(s) 649
MnlI CCTC 7 cut(s) 80, 154, 205, 544, 863, 965, 1020
Mox20I TGGCCA 1 cut(s) 614
Mph1103I ATGCAT 1 cut(s) 734
MroNI GCCGGC 1 cut(s) 539
MscI TGGCCA 1 cut(s) 614
MseI TTAA 2 cut(s) 383, 1102
MslI CAYNNNNRTG 2 cut(s) 150, 575
Msp20I TGGCCA 1 cut(s) 614
MspA1I CMGCKG 1 cut(s) 659
MspI CCGG 4 cut(s) 509, 540, 626, 905
MspR9I CCNGG 4 cut(s) 509, 519, 693, 921
Mva1269I GAATGC 1 cut(s) 985
MvaI CCWGG 3 cut(s) 519, 693, 921
MwoI GCNNNNNNNGC 2 cut(s) 227, 470
NaeI GCCGGC 1 cut(s) 541
NciI CCSGG 1 cut(s) 509
NdeII GATC 7 cut(s) 5, 154, 341, 378, 406, 955, 1076
NgoMIV GCCGGC 1 cut(s) 539
NlaIII CATG 6 cut(s) 155, 190, 254, 714, 732, 1016
NlaIV GGNNCC 3 cut(s) 146, 708, 1061
NmuCI GTSAC 3 cut(s) 46, 485, 719
NsiI ATGCAT 1 cut(s) 734
NspI RCATGY 1 cut(s) 190
OliI CACNNNNGTG 1 cut(s) 575
PciSI GCTCTTC 1 cut(s) 17
PctI GAATGC 1 cut(s) 985
PdiI GCCGGC 1 cut(s) 541
PfeI GAWTC 1 cut(s) 284
PkrI GCNGC 9 cut(s) 220, 271, 331, 394, 658, 856, 916, 940, 1069
PleI GAGTC 2 cut(s) 696, 816
PpsI GAGTC 2 cut(s) 696, 816
PshAI GACNNNNGTC 1 cut(s) 687
Psp6I CCWGG 3 cut(s) 517, 691, 919
PspGI CCWGG 3 cut(s) 517, 691, 919
PspN4I GGNNCC 3 cut(s) 146, 708, 1061
PspPI GGNCC 3 cut(s) 707, 872, 1059
PstNI CAGNNNCTG 1 cut(s) 224
PsuI RGATCY 3 cut(s) 5, 341, 378
PvuII CAGCTG 1 cut(s) 659
RsaI GTAC 3 cut(s) 239, 506, 757
RsaNI GTAC 3 cut(s) 238, 505, 756
RseI CAYNNNNRTG 2 cut(s) 150, 575
SapI GCTCTTC 1 cut(s) 17
SaqAI TTAA 2 cut(s) 383, 1102
SatI GCNGC 9 cut(s) 219, 270, 330, 393, 657, 855, 915, 939, 1068
Sau3AI GATC 7 cut(s) 5, 154, 341, 378, 406, 955, 1076
Sau96I GGNCC 3 cut(s) 707, 872, 1059
SchI GAGTC 2 cut(s) 697, 817
ScrFI CCNGG 4 cut(s) 509, 519, 693, 921
SexAI ACCWGGT 1 cut(s) 919
SfaNI GCATC 2 cut(s) 187, 217
SfcI CTRYAG 5 cut(s) 73, 387, 402, 795, 899
SinI GGWCC 2 cut(s) 707, 1059
SmiMI CAYNNNNRTG 2 cut(s) 150, 575
SmlI CTYRAG 1 cut(s) 288
SmoI CTYRAG 1 cut(s) 288
Sse9I AATT 4 cut(s) 90, 275, 675, 803
SsiI CCGC 1 cut(s) 855
SspMI CTAG 4 cut(s) 434, 536, 645, 867
StyD4I CCNGG 4 cut(s) 507, 517, 691, 919
StyI CCWWGG 2 cut(s) 374, 846
TaaI ACNGT 5 cut(s) 42, 100, 242, 358, 388
TaqI TCGA 2 cut(s) 360, 842
TaqII GACCGA 1 cut(s) 399
TasI AATT 4 cut(s) 90, 275, 675, 803
TatI WGTACW 1 cut(s) 237
TauI GCSGC 1 cut(s) 857
TfiI GAWTC 1 cut(s) 284
Tru1I TTAA 2 cut(s) 383, 1102
Tru9I TTAA 2 cut(s) 383, 1102
TscAI CASTG 5 cut(s) 45, 361, 615, 724, 828
TseFI GTSAC 3 cut(s) 46, 485, 719
TseI GCWGC 8 cut(s) 218, 269, 329, 392, 656, 914, 938, 1067
Tsp45I GTSAC 3 cut(s) 46, 485, 719
TspDTI ATGAA 3 cut(s) 32, 351, 980
TspGWI ACGGA 1 cut(s) 494
TspRI CASTG 5 cut(s) 45, 361, 615, 724, 828
VpaK11BI GGWCC 2 cut(s) 707, 1059
XapI RAATTY 1 cut(s) 275
XbaI TCTAGA 1 cut(s) 866
XceI RCATGY 1 cut(s) 190
XspI CTAG 4 cut(s) 434, 536, 645, 867
Zsp2I ATGCAT 1 cut(s) 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.