RchiOBHm_Chr7g0187651

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001127
Physical Location & Seq
Forward (+)
7442917 .. 7446838
3922 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16752

Sequence Viewer

Length: 2181 bp
ATGGGTAAAAGGCACCACCATGATCCTGAGGTTGTAACCTGTTTGCATCACGACATGCTTGCCTCTGTTGTGGGGAGGAGCTTGGCCTCATTCGAATCTAAATTTACAGAATCTCAAGCTAAGAAGATGGCAGACTTTCCTGGAGTTATTCGTGTTATACCAAATCACTTTCATTCGCTGCAAACAACTAGGAGCTGGGATTATCTAGGGCTCTCTCCTAATTCTCCCAATACCCTTTTAAATGACACAAACTTAGGTGATGGAATTATCATTGGTCTACTGGACACAGGTATATGGCCAGAGTCTGAGGTGTTCAATGATGAGGATCTGGGGCCAATCCCAAGTCAATGGAAGGGTCAGTGTGTATCTGGACAGCAATTCAATGCCTCAACAGCTTGTAACAATAAGCTAATAGGAGCAAAATATTACATTGACGGTTTTCTTGCTGAGAATGAACAACCATTCAACACCACAGACAGCCCGGATTTCTTGTCCCCAAGAGACGTTGTTGGACATGGAACACACACATCTACAATTGCTGGCGGCTCCTTTGTGTACAATGCAAGCTACAAAGGGATTGGCCTTGGAATAGTCAGAGGAGGAGCTCCCCGTGCTCGTTTGGCTATGTACAAAGTGTGCTGGAATGTGCCAAGAGGACAGTGCTCAAATGTTGACATGCTGAAAGCTTTTGATGATGCAATTCATGATGGAGTTGATGTTATATCAGTATCTCTTGGCACTCAACTTCCACTGTTTTCAGAGGTTGATGACCGCGATGCAATTTCCATTGGTTCATTCCATGCTGTTACTAAAGGCATACCTGTTGTTGGTGGAGCTGCAAATGAAGGACCTTCTGCATACACTGTCGAAAACACAGCACCTTGGATCTTAACTGTAGCAGCTTCTACTATAGATCGGTCTTTTCCTACAAATATCACACTAGGGAACAACTCAACCATACTGGGTCAAGCCCTGTTTGCAGGAACGGAAGGTGACTTTACTGGTTTGGTGTACCCGGAGAACCCAGGACTTATTCCTTCACTAGCCGGCGTCTGTGAGGCTCTTATACTCAATAACACACCTGTGGCTGGAAATGTAGTCCTTTGCTTTACATCAGTGGCCAGAAGAACTCCGGTAGCACTTGCTGTATCTAGTGTCAGAGCAGCTGGTGGGGTTGGAGTAATTGTTGCCAAGAGTCCTGGTGATGTCTTGGGTCCATGTAGCAGTGACTTTCCATGCATTGAAGTAGACTACGAGCTTGGTACTCAGATACTATTCTACATTCGCTCCACCAGTTCTCCTGTAGTAAAATTGAGTCCTTCTATGACACTGGTGGGGAAGCCTGTATCGACCAAGGTTGCCGCTTTCTCATCTAGAGGACCTAACTCCATTTCTCCAGCCATACTAAAGCCGGACATAGCTGCACCTGGTGTGAGCATATTAGCTGCCAGTTCTCCTTTTGATCCGTATATGAATGGAGGATTTGCCCTGCATTCAGGAACATCAATGGCAACTCCTCATGTTTCGGGGATTGTGGCACTTCTAAAAGCATTGCACTCCGATTGGTCCCCTGCTGCCATAAGATCAGCAATAACAACAACAGCATGGAAGACTGATCCATTTGGAGAGCCAATATTTGCTGAGGGGTCACCGCAAAAGCTTGCTGATCCATTTGATTATGGAGGTGGGATTGTGAACCCTAACAAGGCTGCAGACCCTGGTCTAATTTATGACATGGGCATTAATCACTACATAATTTACCTATGTGCTGTTGGGTACAACAACTCGGTAATTTCTCAACTTGTTGGGAATTCGACGACATGTTCTAGTACAAAGCCTTCTGTTCTTGATGTGAACCTGCCATCCATTACCATTCCAAATCTAAGAGAGAACATTACGCTCACCAGAAGTGTCACAAATGTTGGTCCAGTTAACTCAACATACAGAGGCCGGATAAGCCTTCCATTGGGAATCAGTGTGGCTGTGAGGCCTGAAACCTTGGTTTTCAACTCGGACATTAAAACAATCTCTTTCACAGTTGCGGTTTCTACCACCCATGAAGTGAACACAGGGTACTACTTTGGAACCCTTGCTTGGACTGATGATGATGGGGGGCATGTGGTGACAATTCCCATATCTGTTAGGACACAAATCATTCGATATTATGCAGATGGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

726

Amino Acids

76.79

Weight (kDa)

5.56

Isoelectric Point (pI)

34.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 86 - 541 2e-41 Subtilase family
fn3_6 PF17766 617 - 714 1.1e-25 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1866
AccB1I GGYRCC 1 cut(s) 12
AccI GTMKAC 2 cut(s) 277, 1248
AccII CGCG 1 cut(s) 774
AciI CCGC 5 cut(s) 543, 772, 1362, 1652, 2042
AclWI GGATC 6 cut(s) 17, 333, 893, 1457, 1610, 1661
AcoI YGGCCR 2 cut(s) 296, 1119
AcsI RAATTY 2 cut(s) 101, 1810
AcyI GRCGYC 1 cut(s) 1050
AdeI CACNNNGTG 1 cut(s) 1430
AfaI GTAC 7 cut(s) 557, 629, 1013, 1264, 1778, 1831, 2075
AfiI CCNNNNNNNGG 5 cut(s) 827, 1088, 1705, 1966, 2094
AflIII ACRYGT 1 cut(s) 1820
AgsI TTSAA 5 cut(s) 316, 382, 466, 1244, 2008
AjnI CCWGG 5 cut(s) 139, 1024, 1198, 1426, 1717
AjuI GAANNNNNNNTTGG 2 cut(s) 2077, 2109
AleI CACNNNNGTG 1 cut(s) 1082
AloI GAACNNNNNNTCC 2 cut(s) 1847, 1879
Alw21I GWGCWC 3 cut(s) 607, 616, 665
Alw26I GTCTC 1 cut(s) 495
AlwI GGATC 6 cut(s) 17, 333, 893, 1457, 1610, 1661
AlwNI CAGNNNCTG 2 cut(s) 305, 1718
AoxI GGCC 7 cut(s) 84, 296, 332, 580, 1119, 1948, 1988
ApeKI GCWGC 8 cut(s) 178, 836, 899, 1163, 1421, 1445, 1574, 1709
ApoI RAATTY 2 cut(s) 101, 1810
AseI ATTAAT 1 cut(s) 1743
AspS9I GGNCC 6 cut(s) 332, 848, 1214, 1379, 1566, 1925
AsuC2I CCSGG 2 cut(s) 482, 1016
AsuHPI GGTGA 6 cut(s) 269, 1004, 1214, 1641, 1894, 2134
AsuII TTCGAA 1 cut(s) 93
AvaII GGWCC 5 cut(s) 848, 1214, 1379, 1566, 1925
AxyI CCTNAGG 1 cut(s) 27
BalI TGGCCA 2 cut(s) 298, 1121
BanI GGYRCC 1 cut(s) 12
BanII GRGCYC 2 cut(s) 213, 607
BarI GAAGNNNNNNTAC 2 cut(s) 1822, 1854
BbsI GAAGAC 1 cut(s) 1616
Bbv12I GWGCWC 3 cut(s) 607, 616, 665
BbvCI CCTCAGC 1 cut(s) 1641
BbvI GCAGC 8 cut(s) 165, 823, 911, 1175, 1408, 1432, 1561, 1696
BccI CCATC 6 cut(s) 121, 254, 701, 1870, 2102, 2165
BcgI CGANNNNNNTGC 2 cut(s) 41, 75
BciT130I CCWGG 5 cut(s) 141, 1026, 1200, 1428, 1719
BcnI CCSGG 2 cut(s) 482, 1016
BcoDI GTCTC 1 cut(s) 495
BfaI CTAG 7 cut(s) 189, 206, 941, 1043, 1152, 1374, 1827
BfmI CTRYAG 4 cut(s) 894, 909, 1302, 1710
BfuAI ACCTGC 1 cut(s) 1866
Bme1390I CCNGG 7 cut(s) 141, 482, 1016, 1026, 1200, 1428, 1719
Bme18I GGWCC 5 cut(s) 848, 1214, 1379, 1566, 1925
BmgT120I GGNCC 6 cut(s) 332, 848, 1214, 1379, 1566, 1925
BmiI GGNNCC 6 cut(s) 14, 333, 547, 1215, 1568, 2086
BmrFI CCNGG 7 cut(s) 141, 482, 1016, 1026, 1200, 1428, 1719
BmrI ACTGGG 1 cut(s) 971
BmsI GCATC 3 cut(s) 55, 685, 766
BmuI ACTGGG 1 cut(s) 971
BoxI GACNNNNGTC 1 cut(s) 1719
BpiI GAAGAC 1 cut(s) 1616
BpmI CTGGAG 2 cut(s) 162, 1380
Bpu10I CCTNAGC 1 cut(s) 1641
Bpu14I TTCGAA 1 cut(s) 93
BpuEI CTTGAG 1 cut(s) 99
BpuMI CCSGG 2 cut(s) 482, 1016
BsaBI GATNNNNATC 1 cut(s) 324
BsaHI GRCGYC 1 cut(s) 1050
BsaJI CCNNGG 6 cut(s) 583, 881, 1024, 1353, 1717, 1998
BsaWI WCCGGW 1 cut(s) 1132
BsaXI ACNNNNNCTCC 8 cut(s) 702, 732, 1170, 1200, 1271, 1282, 1301, 1312
Bsc4I CCNNNNNNNGG 5 cut(s) 827, 1088, 1705, 1966, 2094
Bse118I RCCGGY 1 cut(s) 1046
Bse1I ACTGG 7 cut(s) 285, 966, 1006, 1293, 1335, 1449, 1928
Bse21I CCTNAGG 1 cut(s) 27
Bse3DI GCAATG 1 cut(s) 1550
Bse8I GATNNNNATC 1 cut(s) 324
BseBI CCWGG 5 cut(s) 141, 1026, 1200, 1428, 1719
BseDI CCNNGG 6 cut(s) 583, 881, 1024, 1353, 1717, 1998
BseGI GGATG 1 cut(s) 1862
BseJI GATNNNNATC 1 cut(s) 324
BseLI CCNNNNNNNGG 5 cut(s) 827, 1088, 1705, 1966, 2094
BseMI GCAATG 1 cut(s) 1550
BseMII CTCAG 5 cut(s) 18, 297, 438, 1280, 1632
BseNI ACTGG 7 cut(s) 285, 966, 1006, 1293, 1335, 1449, 1928
BseRI GAGGAG 4 cut(s) 91, 612, 615, 1506
BseXI GCAGC 8 cut(s) 165, 823, 911, 1175, 1408, 1432, 1561, 1696
BseYI CCCAGC 1 cut(s) 195
BsgI GTGCAG 1 cut(s) 1407
Bsh1236I CGCG 1 cut(s) 774
BshFI GGCC 7 cut(s) 86, 298, 334, 582, 1121, 1950, 1990
BshNI GGYRCC 1 cut(s) 12
BsiHKAI GWGCWC 3 cut(s) 607, 616, 665
BsiSI CCGG 6 cut(s) 482, 1016, 1047, 1133, 1412, 1951
BslFI GGGAC 2 cut(s) 478, 1552
BslI CCNNNNNNNGG 5 cut(s) 827, 1088, 1705, 1966, 2094
BsmAI GTCTC 1 cut(s) 495
BsmBI CGTCTC 1 cut(s) 495
BsmFI GGGAC 2 cut(s) 478, 1552
BsmI GAATGC 1 cut(s) 1492
BsnI GGCC 7 cut(s) 86, 298, 334, 582, 1121, 1950, 1990
Bsp119I TTCGAA 1 cut(s) 93
Bsp1286I GDGCHC 4 cut(s) 213, 607, 616, 665
Bsp1407I TGTACA 2 cut(s) 555, 627
Bsp143I GATC 8 cut(s) 22, 325, 885, 913, 1462, 1583, 1615, 1666
BspACI CCGC 5 cut(s) 543, 772, 1362, 1652, 2042
BspANI GGCC 7 cut(s) 86, 298, 334, 582, 1121, 1950, 1990
BspCNI CTCAG 5 cut(s) 19, 298, 439, 1279, 1633
BspFNI CGCG 1 cut(s) 774
BspHI TCATGA 1 cut(s) 703
BspLI GGNNCC 6 cut(s) 14, 333, 547, 1215, 1568, 2086
BspMAI CTGCAG 1 cut(s) 1714
BspMI ACCTGC 1 cut(s) 1866
BspPI GGATC 6 cut(s) 17, 333, 893, 1457, 1610, 1661
BspT104I TTCGAA 1 cut(s) 93
BspT107I GGYRCC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 1550
BsrFI RCCGGY 1 cut(s) 1046
BsrGI TGTACA 2 cut(s) 555, 627
BsrI ACTGG 7 cut(s) 285, 966, 1006, 1293, 1335, 1449, 1928
BssAI RCCGGY 1 cut(s) 1046
BssECI CCNNGG 6 cut(s) 583, 881, 1024, 1353, 1717, 1998
BssMI GATC 8 cut(s) 22, 325, 885, 913, 1462, 1583, 1615, 1666
BssNI GRCGYC 1 cut(s) 1050
BssT1I CCWWGG 4 cut(s) 583, 881, 1353, 1998
Bst2UI CCWGG 5 cut(s) 141, 1026, 1200, 1428, 1719
Bst4CI ACNGT 6 cut(s) 437, 660, 753, 865, 895, 2038
BstACI GRCGYC 1 cut(s) 1050
BstAUI TGTACA 2 cut(s) 555, 627
BstBI TTCGAA 1 cut(s) 93
BstC8I GCNNGC 5 cut(s) 60, 541, 565, 1048, 1662
BstDEI CTNAG 8 cut(s) 27, 120, 253, 306, 447, 1266, 1641, 1883
BstEII GGTNACC 1 cut(s) 1647
BstF5I GGATG 1 cut(s) 1862
BstFNI CGCG 1 cut(s) 774
BstKTI GATC 8 cut(s) 25, 328, 888, 916, 1465, 1586, 1618, 1669
BstMAI GTCTC 1 cut(s) 495
BstMBI GATC 8 cut(s) 22, 325, 885, 913, 1462, 1583, 1615, 1666
BstMWI GCNNNNNNNGC 5 cut(s) 392, 611, 620, 977, 1956
BstNI CCWGG 5 cut(s) 141, 1026, 1200, 1428, 1719
BstNSI RCATGY 4 cut(s) 58, 679, 1824, 2120
BstPAI GACNNNNGTC 1 cut(s) 1719
BstPI GGTNACC 1 cut(s) 1647
BstSCI CCNGG 7 cut(s) 139, 480, 1014, 1024, 1198, 1426, 1717
BstSFI CTRYAG 4 cut(s) 894, 909, 1302, 1710
BstUI CGCG 1 cut(s) 774
BstV1I GCAGC 8 cut(s) 165, 823, 911, 1175, 1408, 1432, 1561, 1696
BstV2I GAAGAC 1 cut(s) 1616
BstX2I RGATCY 2 cut(s) 325, 885
BstXI CCANNNNNNTGG 1 cut(s) 348
BstYI RGATCY 2 cut(s) 325, 885
Bsu36I CCTNAGG 1 cut(s) 27
BsuRI GGCC 7 cut(s) 86, 298, 334, 582, 1121, 1950, 1990
BtgZI GCGATG 1 cut(s) 789
BtsCI GGATG 1 cut(s) 1862
BtsI GCAGTG 1 cut(s) 1231
BtsIMutI CAGTG 8 cut(s) 365, 665, 749, 861, 1122, 1231, 1328, 1981
BveI ACCTGC 1 cut(s) 1866
Cac8I GCNNGC 5 cut(s) 60, 541, 565, 1048, 1662
CaiI CAGNNNCTG 2 cut(s) 305, 1718
CciI TCATGA 1 cut(s) 703
Cfr10I RCCGGY 1 cut(s) 1046
Cfr13I GGNCC 6 cut(s) 332, 848, 1214, 1379, 1566, 1925
CseI GACGC 1 cut(s) 1039
CsiI ACCWGGT 1 cut(s) 1426
Csp6I GTAC 7 cut(s) 556, 628, 1012, 1263, 1777, 1830, 2074
CviQI GTAC 7 cut(s) 556, 628, 1012, 1263, 1777, 1830, 2074
DdeI CTNAG 8 cut(s) 27, 120, 253, 306, 447, 1266, 1641, 1883
DpnI GATC 8 cut(s) 24, 327, 887, 915, 1464, 1585, 1617, 1668
DpnII GATC 8 cut(s) 22, 325, 885, 913, 1462, 1583, 1615, 1666
DraI TTTAAA 1 cut(s) 240
DraIII CACNNNGTG 1 cut(s) 1430
EaeI YGGCCR 2 cut(s) 296, 1119
Ecl136II GAGCTC 1 cut(s) 605
Eco130I CCWWGG 4 cut(s) 583, 881, 1353, 1998
Eco147I AGGCCT 1 cut(s) 1990
Eco24I GRGCYC 2 cut(s) 213, 607
Eco47I GGWCC 5 cut(s) 848, 1214, 1379, 1566, 1925
Eco53kI GAGCTC 1 cut(s) 605
Eco81I CCTNAGG 1 cut(s) 27
Eco91I GGTNACC 1 cut(s) 1647
EcoICRI GAGCTC 1 cut(s) 605
EcoO109I RGGNCCY 2 cut(s) 848, 1379
EcoO65I GGTNACC 1 cut(s) 1647
EcoRI GAATTC 1 cut(s) 1810
EcoRII CCWGG 5 cut(s) 139, 1024, 1198, 1426, 1717
EcoT14I CCWWGG 4 cut(s) 583, 881, 1353, 1998
EcoT22I ATGCAT 1 cut(s) 1241
EcoT38I GRGCYC 2 cut(s) 213, 607
ErhI CCWWGG 4 cut(s) 583, 881, 1353, 1998
Esp3I CGTCTC 1 cut(s) 495
FaqI GGGAC 2 cut(s) 478, 1552
FblI GTMKAC 2 cut(s) 277, 1248
FokI GGATG 1 cut(s) 1849
FriOI GRGCYC 2 cut(s) 213, 607
FspBI CTAG 7 cut(s) 189, 206, 941, 1043, 1152, 1374, 1827
GsaI CCCAGC 1 cut(s) 199
GsuI CTGGAG 2 cut(s) 162, 1380
HaeIII GGCC 7 cut(s) 86, 298, 334, 582, 1121, 1950, 1990
HapII CCGG 6 cut(s) 482, 1016, 1047, 1133, 1412, 1951
HgaI GACGC 1 cut(s) 1039
Hin1I GRCGYC 1 cut(s) 1050
HincII GTYRAC 2 cut(s) 673, 1933
HindII GTYRAC 2 cut(s) 673, 1933
HindIII AAGCTT 2 cut(s) 684, 1658
HinfI GANTC 6 cut(s) 95, 110, 302, 1195, 1315, 1971
HpaI GTTAAC 1 cut(s) 1933
HpaII CCGG 6 cut(s) 482, 1016, 1047, 1133, 1412, 1951
HphI GGTGA 6 cut(s) 269, 1004, 1214, 1641, 1894, 2134
Hpy166II GTNNAC 9 cut(s) 278, 556, 673, 1012, 1249, 1696, 1855, 1933, 2065
Hpy188I TCNGA 7 cut(s) 307, 596, 760, 1160, 1269, 1561, 2014
Hpy188III TCNNGA 7 cut(s) 26, 50, 369, 704, 1374, 1497, 1847
Hpy8I GTNNAC 9 cut(s) 278, 556, 673, 1012, 1249, 1696, 1855, 1933, 2065
Hpy99I CGWCG 1 cut(s) 1819
HpyAV CCTTC 8 cut(s) 346, 839, 861, 983, 1047, 1329, 1848, 1970
HpyCH4III ACNGT 6 cut(s) 437, 660, 753, 865, 895, 2038
HpyCH4IV ACGT 1 cut(s) 504
HpyF10VI GCNNNNNNNGC 5 cut(s) 392, 611, 620, 977, 1956
HpyF3I CTNAG 8 cut(s) 27, 120, 253, 306, 447, 1266, 1641, 1883
HpySE526I ACGT 1 cut(s) 504
Hsp92I GRCGYC 1 cut(s) 1050
KroI GCCGGC 1 cut(s) 1046
KroNI GCCGGC 1 cut(s) 1048
KspAI GTTAAC 1 cut(s) 1933
Kzo9I GATC 8 cut(s) 22, 325, 885, 913, 1462, 1583, 1615, 1666
LmnI GCTCC 8 cut(s) 78, 192, 416, 551, 602, 610, 833, 1292
Lsp1109I GCAGC 8 cut(s) 165, 823, 911, 1175, 1408, 1432, 1561, 1696
LweI GCATC 3 cut(s) 55, 685, 766
MabI ACCWGGT 1 cut(s) 1426
MaeI CTAG 7 cut(s) 189, 206, 941, 1043, 1152, 1374, 1827
MaeII ACGT 1 cut(s) 504
MaeIII GTNAC 8 cut(s) 34, 398, 805, 992, 1226, 1647, 1912, 2122
MalI GATC 8 cut(s) 24, 327, 887, 915, 1464, 1585, 1617, 1668
MboI GATC 8 cut(s) 22, 325, 885, 913, 1462, 1583, 1615, 1666
MboII GAAGA 3 cut(s) 136, 1137, 1621
MfeI CAATTG 2 cut(s) 534, 2176
MflI RGATCY 2 cut(s) 325, 885
MhlI GDGCHC 4 cut(s) 213, 607, 616, 665
MlsI TGGCCA 2 cut(s) 298, 1121
MluNI TGGCCA 2 cut(s) 298, 1121
MlyI GAGTC 3 cut(s) 311, 1204, 1324
MmeI TCCRAC 2 cut(s) 490, 1156
Mox20I TGGCCA 2 cut(s) 298, 1121
Mph1103I ATGCAT 1 cut(s) 1241
MroNI GCCGGC 1 cut(s) 1046
MscI TGGCCA 2 cut(s) 298, 1121
MseI TTAA 5 cut(s) 239, 890, 1743, 1932, 2019
MslI CAYNNNNRTG 2 cut(s) 18, 1082
Msp20I TGGCCA 2 cut(s) 298, 1121
MspA1I CMGCKG 1 cut(s) 1166
MspI CCGG 6 cut(s) 482, 1016, 1047, 1133, 1412, 1951
MspR9I CCNGG 7 cut(s) 141, 482, 1016, 1026, 1200, 1428, 1719
MunI CAATTG 2 cut(s) 534, 2176
Mva1269I GAATGC 1 cut(s) 1492
MvaI CCWGG 5 cut(s) 141, 1026, 1200, 1428, 1719
MvnI CGCG 1 cut(s) 774
MwoI GCNNNNNNNGC 5 cut(s) 392, 611, 620, 977, 1956
NaeI GCCGGC 1 cut(s) 1048
NciI CCSGG 2 cut(s) 482, 1016
NdeII GATC 8 cut(s) 22, 325, 885, 913, 1462, 1583, 1615, 1666
NgoMIV GCCGGC 1 cut(s) 1046
NlaIV GGNNCC 6 cut(s) 14, 333, 547, 1215, 1568, 2086
NmuCI GTSAC 5 cut(s) 992, 1226, 1647, 1912, 2122
NsiI ATGCAT 1 cut(s) 1241
NspI RCATGY 4 cut(s) 58, 679, 1824, 2120
NspV TTCGAA 1 cut(s) 93
OliI CACNNNNGTG 1 cut(s) 1082
PagI TCATGA 1 cut(s) 703
PceI AGGCCT 1 cut(s) 1990
PciI ACATGT 1 cut(s) 1820
PctI GAATGC 1 cut(s) 1492
PdiI GCCGGC 1 cut(s) 1048
PfeI GAWTC 3 cut(s) 95, 110, 1971
PfoI TCCNGGA 1 cut(s) 139
PleI GAGTC 3 cut(s) 310, 1203, 1323
PpsI GAGTC 3 cut(s) 310, 1203, 1323
PpuMI RGGWCCY 2 cut(s) 848, 1379
PscI ACATGT 1 cut(s) 1820
PshAI GACNNNNGTC 1 cut(s) 1719
PshBI ATTAAT 1 cut(s) 1743
Psp124BI GAGCTC 1 cut(s) 607
Psp5II RGGWCCY 2 cut(s) 848, 1379
Psp6I CCWGG 5 cut(s) 139, 1024, 1198, 1426, 1717
PspEI GGTNACC 1 cut(s) 1647
PspFI CCCAGC 1 cut(s) 195
PspGI CCWGG 5 cut(s) 139, 1024, 1198, 1426, 1717
PspN4I GGNNCC 6 cut(s) 14, 333, 547, 1215, 1568, 2086
PspPI GGNCC 6 cut(s) 332, 848, 1214, 1379, 1566, 1925
PspPPI RGGWCCY 2 cut(s) 848, 1379
PsrI GAACNNNNNNTAC 2 cut(s) 2057, 2089
PstI CTGCAG 1 cut(s) 1714
PstNI CAGNNNCTG 2 cut(s) 305, 1718
PsuI RGATCY 2 cut(s) 325, 885
PvuII CAGCTG 1 cut(s) 1166
RsaI GTAC 7 cut(s) 557, 629, 1013, 1264, 1778, 1831, 2075
RsaNI GTAC 7 cut(s) 556, 628, 1012, 1263, 1777, 1830, 2074
RseI CAYNNNNRTG 2 cut(s) 18, 1082
SacI GAGCTC 1 cut(s) 607
SaqAI TTAA 5 cut(s) 239, 890, 1743, 1932, 2019
Sau3AI GATC 8 cut(s) 22, 325, 885, 913, 1462, 1583, 1615, 1666
Sau96I GGNCC 6 cut(s) 332, 848, 1214, 1379, 1566, 1925
SchI GAGTC 3 cut(s) 311, 1204, 1324
ScrFI CCNGG 7 cut(s) 141, 482, 1016, 1026, 1200, 1428, 1719
SduI GDGCHC 4 cut(s) 213, 607, 616, 665
SexAI ACCWGGT 1 cut(s) 1426
SfaNI GCATC 3 cut(s) 55, 685, 766
SfcI CTRYAG 4 cut(s) 894, 909, 1302, 1710
SfuI TTCGAA 1 cut(s) 93
SinI GGWCC 5 cut(s) 848, 1214, 1379, 1566, 1925
SmiMI CAYNNNNRTG 2 cut(s) 18, 1082
SmlI CTYRAG 1 cut(s) 114
SmoI CTYRAG 1 cut(s) 114
SseBI AGGCCT 1 cut(s) 1990
SsiI CCGC 5 cut(s) 543, 772, 1362, 1652, 2042
SspI AATATT 2 cut(s) 425, 1635
SspMI CTAG 7 cut(s) 189, 206, 941, 1043, 1152, 1374, 1827
SstI GAGCTC 1 cut(s) 607
StuI AGGCCT 1 cut(s) 1990
StyD4I CCNGG 7 cut(s) 139, 480, 1014, 1024, 1198, 1426, 1717
StyI CCWWGG 4 cut(s) 583, 881, 1353, 1998
TaaI ACNGT 6 cut(s) 437, 660, 753, 865, 895, 2038
TaiI ACGT 1 cut(s) 507
TaqI TCGA 5 cut(s) 93, 867, 1349, 1814, 2158
TaqII GACCGA 1 cut(s) 906
TatI WGTACW 3 cut(s) 555, 627, 1829
TauI GCSGC 2 cut(s) 546, 1364
TfiI GAWTC 3 cut(s) 95, 110, 1971
Tru1I TTAA 5 cut(s) 239, 890, 1743, 1932, 2019
Tru9I TTAA 5 cut(s) 239, 890, 1743, 1932, 2019
TscAI CASTG 8 cut(s) 365, 665, 756, 868, 1122, 1231, 1335, 1981
TseFI GTSAC 5 cut(s) 992, 1226, 1647, 1912, 2122
TseI GCWGC 8 cut(s) 178, 836, 899, 1163, 1421, 1445, 1574, 1709
Tsp45I GTSAC 5 cut(s) 992, 1226, 1647, 1912, 2122
TspDTI ATGAA 7 cut(s) 161, 468, 692, 783, 858, 1487, 2073
TspGWI ACGGA 2 cut(s) 1001, 1455
TspRI CASTG 8 cut(s) 365, 665, 756, 868, 1122, 1231, 1335, 1981
VpaK11BI GGWCC 5 cut(s) 848, 1214, 1379, 1566, 1925
VspI ATTAAT 1 cut(s) 1743
XapI RAATTY 2 cut(s) 101, 1810
XbaI TCTAGA 1 cut(s) 1373
XceI RCATGY 4 cut(s) 58, 679, 1824, 2120
XmiI GTMKAC 2 cut(s) 277, 1248
XspI CTAG 7 cut(s) 189, 206, 941, 1043, 1152, 1374, 1827
Zsp2I ATGCAT 1 cut(s) 1241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.