Prupe.5G133500_v2.0.a1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0001127
Physical Location & Seq
Forward (+)
12778188 .. 12782714
4527 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G133500.4

Sequence Viewer

Length: 2322 bp
ATGAGCAGCCCAAGTTCAGTACTTGCTATACTTTGCCTTCTCTGCTTTCTTAATGGCCAGGGAATGATAGCGAAGGTGGCTGAAAATAGCCAAGTACACATTGTTTATCTGGGAGAGAGGCAGCATGACAACCCCAAGTTGCTCACAGATTCACATCATGATTTGCTTGCCACCATAGCGGGAAGCAAGGAATTGGCCTCAGAATTAATGGTATACAGTTATAGACATGGTTTCTCTGGGTTTGCAGCCAAGCTCACAGAGTCTCAAGCCCAAAAACTTTCAGAGCTGCCTGGTGTTGTTCGAGTCATACCAAATAGTCTTCACAAGCTGCAAACAACTAGGAGTTGGGATTTTCTTGGCCTCTCTTCTCAATCCCCTTCCAATATTCTTCACAAGAGCAGCATGGGTGATGGAGTTATCATAGGTGTCCTTGACACAGGTATTTGGCCGGAGTCTGAATCATTCAATGAAAAAGGCCTGGGGCCAGTTCCATCCCACTGGAAGGGTGTATGTGAATCTGGAGACAGATTCAATGCTACAAAACACTGCAATAGAAAAATCATTGGAGCCCGCTGGTTCATTGATGGACTTCTTACCGAGTATGGAAAACCACTGAACAGGTCTACAGAGTTCTTGTCTCCCAGAGACGCACATGGACATGGTACTCACACTTCTAGCACTGCAGCTGGTTCTTTTGTGACCAATGTGAGCTACAAGGGCCTTGGTCATGGGACAATCAAAGGGGGTGCCCCAAATGCTCGGTTGGCCATTTACAAGGTTTGCTGGAAAGTGCTTGGAGGCCAATGCTCATCAGCTGATATATTGAAAGCTTTTGATGAAGCCATACATGATGGGGTTGATGTGTTGTCATTGTCAATTGGGTCTTCAATTCCCCTCTTCTCAGAGGTTGATGAGCGTGACGGCATTGCAACCGGTTCCTTTCATGCTGTGGCCAGGGGAATTACTGTTGTTTGTGGAGCAGCAAATGATGGACCTTCAGCTGAGACGGTTCAGAACACAGCGCCATGGATCATAACCGTAGCAGCAAGCACCATGGATCGATCATTTCCTACTTCGATAACTTTAGGAAATAACAAAACTTTCTTGGGACAAGCTATGTTTACAGGGCCAGAGATTGGATTTGCAAGCTTGATATACCCAGAATCCAAAGGGCTTGACCCTACTGCTGCCGGTGTGTGCCAATCCCTGTCACTTAACAAAACTATGGTAGCTGGAAAGGTGGTTCTTTGCTTCACTACAGTCAGTCGTAGGACAGCTATAACAAGTGCTTCAGCAGCTGTTAAAGAAGCCGGCGGTGTTGGCCTCATCGTAGCAAAGAATCCAAGTGACGCCTTATATCCATGTAATGAAGACTTCCCATGCACTGAAGTTGACTATGAAATTGGCACCCGAATACTGTTTTACATCCGGTCTACCAGATCTCCTCTTGTAAAATTGAGACCTCCTAAAACATTTATTGGCAAGCCACTCTCAGCCAAGGTGGCTTATTTTTCATCTAGGGGGCCAAACTCCATTACACCTGCAATTCTTAAGCCAGATATAGCTGCCCCTGGTGTGAACATTCTGGCTGCAACTTCTCCACTTGATGCACTAGGGGAGGGTGGATATGTCATGCACTCAGGAACATCAATGTCAACTCCTCATGTCGCAGGCATTGTGGCTCTTCTCAAAGCAATGCATCCCAATTGGTCTCCGGCCGCCATTAAATCGGCACTTGTCACAACTGCATGGAGGAATGGCCCGTCTGGTTTACCTATCTTTGCAGAGGGATCGCCTCAAAAGCTGGCAAATCCGTTCGATTTTGGAGGCGGCATTATGAACCCCAATGGAGCAGCAGACCCTGGTTTAGTATATGACATAGGCAAAGCCGGCTACATGCAATATCTCTGTTCAAGGGGCTACAACAACTCTGCCATCTCTAGGCTTGTAGGGCAGAACACAAAATGTCCCATTAAGAAGCCCTCTATCCTTGACATGAACCTACCCTCCATAACCATACCAAGTCTCAAAAATCCCATCACAATAAAAAGAAGTGTAACAAATGTGGGAGCCCCTGAATCCATTTACAGAGCTACCATTGAGACTCCATTTGGTACAATTGTATCTGTAAATCCCAATGCTCTGGTCTTCAACTCTACAGTTCGAAAGCTGGATTTCACGATCACAATCTCCACGATCCACAGGATGAATACTGGATACTATTTTGGAAGCCTTAGCTGGGCTGATGGAGTGCATGTTGTAAAGATTCCTTTGTCTGTGAGGACAGAGTTTTTGCAACCTTATGATGCAAATGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

774

Amino Acids

82.23

Weight (kDa)

8.69

Isoelectric Point (pI)

37.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1549
Acc36I ACCTGC 1 cut(s) 1549
AccB1I GGYRCC 2 cut(s) 746, 1406
AccB7I CCANNNNNTGG 1 cut(s) 1136
AccI GTMKAC 3 cut(s) 213, 623, 1433
AciI CCGC 5 cut(s) 179, 571, 1314, 1719, 1830
AclWI GGATC 4 cut(s) 1037, 1065, 1798, 2191
AcoI YGGCCR 5 cut(s) 55, 446, 765, 951, 1716
AcuI CTGAAG 3 cut(s) 981, 1275, 1407
AcyI GRCGYC 1 cut(s) 1350
AfaI GTAC 4 cut(s) 21, 96, 664, 2116
AfiI CCNNNNNNNGG 4 cut(s) 502, 1136, 1941, 2239
AflII CTTAAG 1 cut(s) 1550
AgeI ACCGGT 1 cut(s) 932
AgsI TTSAA 6 cut(s) 466, 532, 826, 888, 1914, 2152
AjnI CCWGG 6 cut(s) 57, 289, 477, 953, 1570, 1861
AloI GAACNNNNNNTCC 4 cut(s) 1227, 1259, 1991, 2023
Alw26I GTCTC 9 cut(s) 267, 516, 639, 642, 998, 1453, 1716, 2030, 2096
AlwI GGATC 4 cut(s) 1037, 1065, 1798, 2191
AlwNI CAGNNNCTG 2 cut(s) 1298, 1862
ArsI GACNNNNNNTTYG 2 cut(s) 2275, 2307
AseI ATTAAT 1 cut(s) 206
AsiGI ACCGGT 1 cut(s) 932
AspLEI GCGC 1 cut(s) 1024
AspS9I GGNCC 6 cut(s) 482, 718, 992, 1127, 1523, 1760
AsuHPI GGTGA 1 cut(s) 419
AsuII TTCGAA 1 cut(s) 2164
AvaII GGWCC 1 cut(s) 992
BaeGI GKGCMC 1 cut(s) 751
BaeI ACNNNNGTAYC 4 cut(s) 2106, 2106, 2139, 2139
BalI TGGCCA 3 cut(s) 57, 767, 953
BanI GGYRCC 2 cut(s) 746, 1406
BanII GRGCYC 2 cut(s) 571, 2074
BarI GAAGNNNNNNTAC 2 cut(s) 655, 687
BbsI GAAGAC 4 cut(s) 311, 876, 1377, 2140
BccI CCATC 8 cut(s) 404, 499, 578, 845, 983, 1943, 2045, 2240
BceAI ACGGC 1 cut(s) 937
BcgI CGANNNNNNTGC 2 cut(s) 1773, 1807
BciT130I CCWGG 6 cut(s) 59, 291, 479, 955, 1572, 1863
BciVI GTATCC 1 cut(s) 2210
BcoDI GTCTC 9 cut(s) 267, 516, 639, 642, 998, 1453, 1716, 2030, 2096
BfaI CTAG 5 cut(s) 339, 675, 1518, 1613, 1941
BfmI CTRYAG 4 cut(s) 624, 681, 1257, 2157
BfoI RGCGCY 1 cut(s) 1025
BfrI CTTAAG 1 cut(s) 1550
BfuAI ACCTGC 1 cut(s) 1549
BfuI GTATCC 1 cut(s) 2210
BglI GCCNNNNNGGC 1 cut(s) 1502
BglII AGATCT 1 cut(s) 1439
BmcAI AGTACT 1 cut(s) 21
Bme1390I CCNGG 6 cut(s) 59, 291, 479, 955, 1572, 1863
Bme18I GGWCC 1 cut(s) 992
BmgT120I GGNCC 6 cut(s) 482, 718, 992, 1127, 1523, 1760
BmiI GGNNCC 7 cut(s) 483, 568, 748, 937, 1408, 1524, 2071
BmrFI CCNGG 6 cut(s) 59, 291, 479, 955, 1572, 1863
BmsI GCATC 3 cut(s) 1597, 1708, 2296
BpiI GAAGAC 4 cut(s) 311, 876, 1377, 2140
BpmI CTGGAG 1 cut(s) 540
Bpu10I CCTNAGC 1 cut(s) 2234
Bpu14I TTCGAA 1 cut(s) 2164
BpuEI CTTGAG 1 cut(s) 249
Bsa29I ATCGAT 1 cut(s) 1060
BsaBI GATNNNNATC 2 cut(s) 153, 2186
BsaHI GRCGYC 1 cut(s) 1350
BsaI GGTCTC 2 cut(s) 1453, 1716
BsaJI CCNNGG 9 cut(s) 58, 478, 721, 954, 1025, 1053, 1497, 1570, 1861
BsaWI WCCGGW 2 cut(s) 932, 1428
BsaXI ACNNNNNCTCC 6 cut(s) 1426, 1456, 1991, 2021, 2241, 2271
Bsc4I CCNNNNNNNGG 4 cut(s) 502, 1136, 1941, 2239
Bse118I RCCGGY 4 cut(s) 932, 1190, 1310, 1889
Bse1I ACTGG 3 cut(s) 485, 503, 2218
Bse3DI GCAATG 2 cut(s) 924, 1701
Bse8I GATNNNNATC 2 cut(s) 153, 2186
BseBI CCWGG 6 cut(s) 59, 291, 479, 955, 1572, 1863
BseCI ATCGAT 1 cut(s) 1060
BseDI CCNNGG 9 cut(s) 58, 478, 721, 954, 1025, 1053, 1497, 1570, 1861
BseGI GGATG 4 cut(s) 491, 1425, 1699, 2211
BseJI GATNNNNATC 2 cut(s) 153, 2186
BseLI CCNNNNNNNGG 4 cut(s) 502, 1136, 1941, 2239
BseMI GCAATG 2 cut(s) 924, 1701
BseMII CTCAG 5 cut(s) 213, 915, 993, 1506, 1653
BseNI ACTGG 3 cut(s) 485, 503, 2218
BseRI GAGGAG 2 cut(s) 1434, 1650
BseSI GKGCMC 1 cut(s) 751
BseX3I CGGCCG 1 cut(s) 1716
BseYI CCCAGC 1 cut(s) 2238
Bsh1285I CGRYCG 1 cut(s) 1719
BshNI GGYRCC 2 cut(s) 746, 1406
BshTI ACCGGT 1 cut(s) 932
BshVI ATCGAT 1 cut(s) 1060
BsiEI CGRYCG 1 cut(s) 1719
BsiSI CCGG 7 cut(s) 449, 933, 1191, 1311, 1429, 1715, 1890
BslFI GGGAC 3 cut(s) 745, 1122, 1953
BslI CCNNNNNNNGG 4 cut(s) 502, 1136, 1941, 2239
BsmAI GTCTC 9 cut(s) 267, 516, 639, 642, 998, 1453, 1716, 2030, 2096
BsmBI CGTCTC 2 cut(s) 639, 998
BsmFI GGGAC 3 cut(s) 745, 1122, 1953
Bso31I GGTCTC 2 cut(s) 1453, 1716
Bsp119I TTCGAA 1 cut(s) 2164
Bsp1286I GDGCHC 3 cut(s) 571, 751, 2074
Bsp143I GATC 7 cut(s) 1029, 1057, 1061, 1439, 1790, 2181, 2196
Bsp19I CCATGG 2 cut(s) 1025, 1053
BspACI CCGC 5 cut(s) 179, 571, 1314, 1719, 1830
BspCNI CTCAG 5 cut(s) 212, 914, 994, 1505, 1652
BspDI ATCGAT 1 cut(s) 1060
BspHI TCATGA 1 cut(s) 157
BspLI GGNNCC 7 cut(s) 483, 568, 748, 937, 1408, 1524, 2071
BspMAI CTGCAG 1 cut(s) 685
BspMI ACCTGC 1 cut(s) 1549
BspPI GGATC 4 cut(s) 1037, 1065, 1798, 2191
BspQI GCTCTTC 1 cut(s) 1689
BspT104I TTCGAA 1 cut(s) 2164
BspT107I GGYRCC 2 cut(s) 746, 1406
BspTI CTTAAG 1 cut(s) 1550
BspTNI GGTCTC 2 cut(s) 1453, 1716
BsrDI GCAATG 2 cut(s) 924, 1701
BsrFI RCCGGY 4 cut(s) 932, 1190, 1310, 1889
BsrI ACTGG 3 cut(s) 485, 503, 2218
BssAI RCCGGY 4 cut(s) 932, 1190, 1310, 1889
BssECI CCNNGG 9 cut(s) 58, 478, 721, 954, 1025, 1053, 1497, 1570, 1861
BssMI GATC 7 cut(s) 1029, 1057, 1061, 1439, 1790, 2181, 2196
BssNAI GTATAC 1 cut(s) 214
BssNI GRCGYC 1 cut(s) 1350
BssT1I CCWWGG 4 cut(s) 721, 1025, 1053, 1497
Bst1107I GTATAC 1 cut(s) 214
Bst2UI CCWGG 6 cut(s) 59, 291, 479, 955, 1572, 1863
Bst4CI ACNGT 7 cut(s) 218, 967, 1009, 1039, 1261, 1419, 2161
Bst6I CTCTTC 3 cut(s) 370, 902, 1689
BstACI GRCGYC 1 cut(s) 1350
BstAFI CTTAAG 1 cut(s) 1550
BstBI TTCGAA 1 cut(s) 2164
BstC8I GCNNGC 9 cut(s) 168, 571, 1048, 1147, 1312, 1484, 1672, 1806, 1891
BstDEI CTNAG 6 cut(s) 199, 901, 1002, 1492, 1639, 2234
BstDSI CCRYGG 2 cut(s) 1025, 1053
BstF5I GGATG 4 cut(s) 491, 1425, 1699, 2211
BstH2I RGCGCY 1 cut(s) 1025
BstHHI GCGC 1 cut(s) 1024
BstKTI GATC 7 cut(s) 1032, 1060, 1064, 1442, 1793, 2184, 2199
BstMAI GTCTC 9 cut(s) 267, 516, 639, 642, 998, 1453, 1716, 2030, 2096
BstMBI GATC 7 cut(s) 1029, 1057, 1061, 1439, 1790, 2181, 2196
BstMCI CGRYCG 1 cut(s) 1719
BstNI CCWGG 6 cut(s) 59, 291, 479, 955, 1572, 1863
BstNSI RCATGY 2 cut(s) 1900, 2258
BstSCI CCNGG 6 cut(s) 57, 289, 477, 953, 1570, 1861
BstSFI CTRYAG 4 cut(s) 624, 681, 1257, 2157
BstSLI GKGCMC 1 cut(s) 751
BstV2I GAAGAC 4 cut(s) 311, 876, 1377, 2140
BstX2I RGATCY 1 cut(s) 1439
BstXI CCANNNNNNTGG 2 cut(s) 498, 2143
BstYI RGATCY 1 cut(s) 1439
BstZ17I GTATAC 1 cut(s) 214
BstZI CGGCCG 1 cut(s) 1716
Bsu15I ATCGAT 1 cut(s) 1060
BsuI GTATCC 1 cut(s) 2210
BsuTUI ATCGAT 1 cut(s) 1060
BtgI CCRYGG 2 cut(s) 1025, 1053
BtsCI GGATG 4 cut(s) 491, 1425, 1699, 2211
BtsI GCAGTG 2 cut(s) 544, 678
BtsIMutI CAGTG 5 cut(s) 496, 544, 611, 678, 1383
BveI ACCTGC 1 cut(s) 1549
Cac8I GCNNGC 9 cut(s) 168, 571, 1048, 1147, 1312, 1484, 1672, 1806, 1891
CaiI CAGNNNCTG 2 cut(s) 1298, 1862
CciI TCATGA 1 cut(s) 157
CfoI GCGC 1 cut(s) 1024
Cfr10I RCCGGY 4 cut(s) 932, 1190, 1310, 1889
Cfr13I GGNCC 6 cut(s) 482, 718, 992, 1127, 1523, 1760
ClaI ATCGAT 1 cut(s) 1060
CseI GACGC 2 cut(s) 656, 1358
Csp6I GTAC 4 cut(s) 20, 95, 663, 2115
CspAI ACCGGT 1 cut(s) 932
CviQI GTAC 4 cut(s) 20, 95, 663, 2115
DdeI CTNAG 6 cut(s) 199, 901, 1002, 1492, 1639, 2234
DpnI GATC 7 cut(s) 1031, 1059, 1063, 1441, 1792, 2183, 2198
DpnII GATC 7 cut(s) 1029, 1057, 1061, 1439, 1790, 2181, 2196
EaeI YGGCCR 5 cut(s) 55, 446, 765, 951, 1716
EagI CGGCCG 1 cut(s) 1716
Eam1104I CTCTTC 3 cut(s) 370, 902, 1689
EarI CTCTTC 3 cut(s) 370, 902, 1689
EclXI CGGCCG 1 cut(s) 1716
Eco130I CCWWGG 4 cut(s) 721, 1025, 1053, 1497
Eco147I AGGCCT 1 cut(s) 477
Eco24I GRGCYC 2 cut(s) 571, 2074
Eco31I GGTCTC 2 cut(s) 1453, 1716
Eco47I GGWCC 1 cut(s) 992
Eco52I CGGCCG 1 cut(s) 1716
Eco57I CTGAAG 3 cut(s) 981, 1275, 1407
EcoO109I RGGNCCY 1 cut(s) 718
EcoRII CCWGG 6 cut(s) 57, 289, 477, 953, 1570, 1861
EcoT14I CCWWGG 4 cut(s) 721, 1025, 1053, 1497
EcoT22I ATGCAT 1 cut(s) 1701
EcoT38I GRGCYC 2 cut(s) 571, 2074
ErhI CCWWGG 4 cut(s) 721, 1025, 1053, 1497
Esp3I CGTCTC 2 cut(s) 639, 998
FaqI GGGAC 3 cut(s) 745, 1122, 1953
FauI CCCGC 2 cut(s) 172, 578
FblI GTMKAC 3 cut(s) 213, 623, 1433
FokI GGATG 4 cut(s) 478, 1412, 1686, 2218
FriOI GRGCYC 2 cut(s) 571, 2074
FspBI CTAG 5 cut(s) 339, 675, 1518, 1613, 1941
GlaI GCGC 1 cut(s) 1023
GsaI CCCAGC 1 cut(s) 2242
GsuI CTGGAG 1 cut(s) 540
HaeII RGCGCY 1 cut(s) 1025
HapII CCGG 7 cut(s) 449, 933, 1191, 1311, 1429, 1715, 1890
HgaI GACGC 2 cut(s) 656, 1358
HhaI GCGC 1 cut(s) 1024
Hin1I GRCGYC 1 cut(s) 1350
Hin6I GCGC 1 cut(s) 1022
HinP1I GCGC 1 cut(s) 1022
HincII GTYRAC 2 cut(s) 1393, 1656
HindII GTYRAC 2 cut(s) 1393, 1656
HindIII AAGCTT 2 cut(s) 828, 1147
HpaII CCGG 7 cut(s) 449, 933, 1191, 1311, 1429, 1715, 1890
HphI GGTGA 1 cut(s) 419
Hpy166II GTNNAC 9 cut(s) 97, 214, 624, 1122, 1393, 1434, 1579, 1656, 1772
Hpy188I TCNGA 5 cut(s) 202, 283, 457, 904, 1014
Hpy188III TCNNGA 4 cut(s) 158, 519, 1641, 2179
Hpy8I GTNNAC 9 cut(s) 97, 214, 624, 1122, 1393, 1434, 1579, 1656, 1772
HpyAV CCTTC 5 cut(s) 47, 67, 387, 496, 1005
HpyCH4III ACNGT 7 cut(s) 218, 967, 1009, 1039, 1261, 1419, 2161
HpyF3I CTNAG 6 cut(s) 199, 901, 1002, 1492, 1639, 2234
Hsp92I GRCGYC 1 cut(s) 1350
HspAI GCGC 1 cut(s) 1022
KroI GCCGGC 2 cut(s) 1310, 1889
KroNI GCCGGC 2 cut(s) 1312, 1891
Kzo9I GATC 7 cut(s) 1029, 1057, 1061, 1439, 1790, 2181, 2196
LguI GCTCTTC 1 cut(s) 1689
LmnI GCTCC 4 cut(s) 566, 977, 1850, 2069
LweI GCATC 3 cut(s) 1597, 1708, 2296
MaeI CTAG 5 cut(s) 339, 675, 1518, 1613, 1941
MaeIII GTNAC 6 cut(s) 697, 917, 1209, 1346, 1738, 2056
MalI GATC 7 cut(s) 1031, 1059, 1063, 1441, 1792, 2183, 2198
MboI GATC 7 cut(s) 1029, 1057, 1061, 1439, 1790, 2181, 2196
MboII GAAGA 8 cut(s) 311, 357, 380, 876, 889, 1382, 1676, 2140
MfeI CAATTG 3 cut(s) 876, 1705, 2118
MflI RGATCY 1 cut(s) 1439
MhlI GDGCHC 3 cut(s) 571, 751, 2074
MlsI TGGCCA 3 cut(s) 57, 767, 953
MluNI TGGCCA 3 cut(s) 57, 767, 953
MlyI GAGTC 4 cut(s) 269, 312, 461, 2098
Mox20I TGGCCA 3 cut(s) 57, 767, 953
Mph1103I ATGCAT 1 cut(s) 1701
MroNI GCCGGC 2 cut(s) 1310, 1889
MscI TGGCCA 3 cut(s) 57, 767, 953
MseI TTAA 7 cut(s) 51, 206, 1215, 1302, 1551, 1725, 1974
MslI CAYNNNNRTG 1 cut(s) 657
Msp20I TGGCCA 3 cut(s) 57, 767, 953
MspA1I CMGCKG 5 cut(s) 573, 686, 815, 1001, 1298
MspCI CTTAAG 1 cut(s) 1550
MspI CCGG 7 cut(s) 449, 933, 1191, 1311, 1429, 1715, 1890
MspR9I CCNGG 6 cut(s) 59, 291, 479, 955, 1572, 1863
MunI CAATTG 3 cut(s) 876, 1705, 2118
MvaI CCWGG 6 cut(s) 59, 291, 479, 955, 1572, 1863
NaeI GCCGGC 2 cut(s) 1312, 1891
NcoI CCATGG 2 cut(s) 1025, 1053
NdeII GATC 7 cut(s) 1029, 1057, 1061, 1439, 1790, 2181, 2196
NgoMIV GCCGGC 2 cut(s) 1310, 1889
NlaIV GGNNCC 7 cut(s) 483, 568, 748, 937, 1408, 1524, 2071
NmuCI GTSAC 5 cut(s) 697, 917, 1209, 1346, 1738
NsiI ATGCAT 1 cut(s) 1701
NspI RCATGY 2 cut(s) 1900, 2258
NspV TTCGAA 1 cut(s) 2164
PagI TCATGA 1 cut(s) 157
PaqCI CACCTGC 1 cut(s) 1549
PceI AGGCCT 1 cut(s) 477
PciSI GCTCTTC 1 cut(s) 1689
PdiI GCCGGC 2 cut(s) 1312, 1891
PfeI GAWTC 8 cut(s) 149, 458, 515, 528, 1163, 1339, 2078, 2266
PflMI CCANNNNNTGG 1 cut(s) 1136
PinAI ACCGGT 1 cut(s) 932
PleI GAGTC 4 cut(s) 268, 311, 460, 2098
PpsI GAGTC 4 cut(s) 268, 311, 460, 2098
PshBI ATTAAT 1 cut(s) 206
Psp6I CCWGG 6 cut(s) 57, 289, 477, 953, 1570, 1861
PspFI CCCAGC 1 cut(s) 2238
PspGI CCWGG 6 cut(s) 57, 289, 477, 953, 1570, 1861
PspN4I GGNNCC 7 cut(s) 483, 568, 748, 937, 1408, 1524, 2071
PspPI GGNCC 6 cut(s) 482, 718, 992, 1127, 1523, 1760
PstI CTGCAG 1 cut(s) 685
PstNI CAGNNNCTG 2 cut(s) 1298, 1862
PsuI RGATCY 1 cut(s) 1439
PvuII CAGCTG 4 cut(s) 686, 815, 1001, 1298
RsaI GTAC 4 cut(s) 21, 96, 664, 2116
RsaNI GTAC 4 cut(s) 20, 95, 663, 2115
RseI CAYNNNNRTG 1 cut(s) 657
SapI GCTCTTC 1 cut(s) 1689
SaqAI TTAA 7 cut(s) 51, 206, 1215, 1302, 1551, 1725, 1974
Sau3AI GATC 7 cut(s) 1029, 1057, 1061, 1439, 1790, 2181, 2196
Sau96I GGNCC 6 cut(s) 482, 718, 992, 1127, 1523, 1760
ScaI AGTACT 1 cut(s) 21
SchI GAGTC 4 cut(s) 269, 312, 461, 2098
ScrFI CCNGG 6 cut(s) 59, 291, 479, 955, 1572, 1863
SduI GDGCHC 3 cut(s) 571, 751, 2074
SfaNI GCATC 3 cut(s) 1597, 1708, 2296
SfcI CTRYAG 4 cut(s) 624, 681, 1257, 2157
SfuI TTCGAA 1 cut(s) 2164
SinI GGWCC 1 cut(s) 992
SmiMI CAYNNNNRTG 1 cut(s) 657
SmlI CTYRAG 2 cut(s) 264, 1550
SmoI CTYRAG 2 cut(s) 264, 1550
SseBI AGGCCT 1 cut(s) 477
SsiI CCGC 5 cut(s) 179, 571, 1314, 1719, 1830
SspI AATATT 1 cut(s) 385
SspMI CTAG 5 cut(s) 339, 675, 1518, 1613, 1941
StuI AGGCCT 1 cut(s) 477
StyD4I CCNGG 6 cut(s) 57, 289, 477, 953, 1570, 1861
StyI CCWWGG 4 cut(s) 721, 1025, 1053, 1497
TaaI ACNGT 7 cut(s) 218, 967, 1009, 1039, 1261, 1419, 2161
TaqI TCGA 5 cut(s) 301, 1060, 1076, 1818, 2164
TatI WGTACW 2 cut(s) 19, 94
TauI GCSGC 2 cut(s) 1721, 1833
TfiI GAWTC 8 cut(s) 149, 458, 515, 528, 1163, 1339, 2078, 2266
Tru1I TTAA 7 cut(s) 51, 206, 1215, 1302, 1551, 1725, 1974
Tru9I TTAA 7 cut(s) 51, 206, 1215, 1302, 1551, 1725, 1974
TscAI CASTG 5 cut(s) 503, 551, 618, 685, 1390
TseFI GTSAC 5 cut(s) 697, 917, 1209, 1346, 1738
Tsp45I GTSAC 5 cut(s) 697, 917, 1209, 1346, 1738
TspGWI ACGGA 1 cut(s) 1803
TspRI CASTG 5 cut(s) 503, 551, 618, 685, 1390
Van91I CCANNNNNTGG 1 cut(s) 1136
Vha464I CTTAAG 1 cut(s) 1550
VpaK11BI GGWCC 1 cut(s) 992
VspI ATTAAT 1 cut(s) 206
XceI RCATGY 2 cut(s) 1900, 2258
XmiI GTMKAC 3 cut(s) 213, 623, 1433
XspI CTAG 5 cut(s) 339, 675, 1518, 1613, 1941
ZrmI AGTACT 1 cut(s) 21
Zsp2I ATGCAT 1 cut(s) 1701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.