MD00G1035000.v1.1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0001127
Physical Location & Seq
Forward (+)
6100399 .. 6104245
3847 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1035000.v1.1.491

Sequence Viewer

Length: 2310 bp
ATGAGCAGCTCAAGTTCAATACTCGCTCTACTCAGCCTTATCTGTTTTACTGGTCAGGGATTGATAACAAGGGTGGCAGCAAAGAGCCATGTACACATTGTTTATATGGGAGATAGGCAGCATGACAACACCAAGTTAATCACAGATTCACATCATGACTTGCTTGCCACTGTCGTGGGAAGCAGGGAATTAGCCTCGGAATTGATGGTATACAGTTACAAACATGGTTTCTCTGGGTTTGCAGCCAAGCTCACGGAGTCTCAAACCCAACAACTTTCAGAGTTGCCTGGTGTTGTACGAATCATACCAAATAGCCTTCATAAGCTGGAAACAACTCGGAGTTGGGATTTTCTTGGCCTCTCACCTCACTCTCCTTCCAATATTCTTCCCAAGAGCAACATGGGGGATGGAGTTATCATAGGTGTTCTTGACACAGGTATATGGCCGGAGTCTGATTCATTCAATGAAAAAGGCCTCGGGCCTGTTCCATCTCATTGGAATGGTGTTTGTGAATCCGGGGACAATTTCAATGCTACAATACATTGCAATAAAAAAATCATCGGAGCCCGTTGGTTTATCAATGGACTTCTTGCCGAGTATGCAAAGCCATTGGACAAAGAATTCCATTCTCCTAGAGATGCACATGGACATGGTACTCATACTTCAAGCACTGCAGCTGGTTCTTTTGTGGCTAATATCAGCTACAAGGGCCTTGGTCTTGGTACAATTAGAGGCGGTGCTCCAAAGGCTCGGTTGGCCATTTACAAGGTTTGTTGGAATGTGCTTGGAGGCAAATGCTCAACAGCTGATATGTTGAAAGCTTTTGACGAAGCAATACATGATGGGGTTGATGTGTTGTCATTGTCAATTGGGCATCCTATTCCCCTCTTCTCAGATGTTGATGAGCGTGATGGCATTGCAACCGGTTCCTTTCATGCTGTGGCTAAGGGGATTACTGTTGTGTGTGGAGCTGGAAATAATGGACCTTCAGCACAAACGGTTTCGAACACAGCACCGTGGATCATAACTGTTGCAGCAAGCACCATGGATCGAGAATTTCCTACTTCTATAACTTTAGGAAATAATAAAACTTTCCTGGGTCAAGCTATGTTTACAGGGACAGAGATTGGATTTACAAGCTTGGTATACCCAGAGTCTAAAGGGCTTACCACTAGAGGTGTGTGTGAATCCCTCTCACTTAACAAAACTATAGTAGTTGGAAAGGTGGTTCTCTGCTTCACTACAATGGGTCGTCGGGCGATAACAAATGCTTCAGCAGTTGTGAAAGAAGCAGGAGGTGTTGGCCTAATCATCGCAAAGAATCCAAGTGATGCTTTATATCCATGTAATGAAGACTTTCCATGCATTGAAGTTGACTATGAGATTGGCACTCGAATTCTATTTTATATCCGGTCTACCAGATATCCTCTTGTAAAGCTGATCCCTCCTCAAACAATTGTGGGCAAGCCACTCTCTGCCAAGGTGGCTTATTTTTCCTCTAGGGGACCAAACTCCATAACACCAGCAACTCTCAAGCCAGATATAGCCGCGCCTGGTGTGAACATACTAGCAGCAACTTCTCCACTTTATTCATTTGTGGAGGGTGGATATGCAATGATGTCTGGAACATCAATGTCAACTCCTCATGTCACAGGCATTGTGGCTCTTATCAAGAGAATGCATCCTAATTGGTCTCCAGCAGCCATCAAATCAGCATTAGTAACAACTGCATGGAGGAACGGCCCATCTGGTTTACCGATATTTGCAGAAGGATCGCCTCAAAAGTTGGCAAATTCATTTGACTTCGGAGGTGGTCTGGTGAATCCCAATGGCGCAGCAGAGCCTGGACTAGTATACGACATGGGTGCAGCAGACTACATGGAATATCTTTGTGCAAGGGCGTACAACAACTCTGCCATCTCTCGACTTACAGGGAAAAAGACAACATGTCCCGTGAAGAAGCCCTCTATCCTTGACGTTAACCTACCTTCCGTGACCATACCTAGTCTGAGAAATCCCGTCACAGTAAAAAGAACTGTAACAAATGTTGGAGCCCCTGAGTCCATTTACAAAGCTACCATTGAGCCTCCATTCGGTACAATTGTATATGTGAACCCTACTGCTCTGGTCTTCAATTCCACAGTTGAAAAACTCACTTTCACAATCACAATCTCCGCAATCCACGAGATGAACACTGGTTACTATTTTGGAAGCCTTACTTGGGTTGACGGAGTGCATGCCGTGAGGATTCCTTTGTCTGTGAGAACTGAGTTCTTACAACCTCATGATGATGATGATGATGAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

770

Amino Acids

82.15

Weight (kDa)

7.07

Isoelectric Point (pI)

33.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 31 - 109 1.9e-17 Peptidase inhibitor I9
Peptidase_S8 PF00082 135 - 585 4.1e-48 Subtilase family
fn3_6 PF17766 659 - 754 2.6e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 4 cut(s) 210, 1146, 1415, 1854
AccII CGCG 1 cut(s) 1550
AciI CCGC 3 cut(s) 735, 1548, 2175
AclWI GGATC 4 cut(s) 1028, 1056, 1435, 1780
AcoI YGGCCR 2 cut(s) 443, 756
AcsI RAATTY 4 cut(s) 620, 1055, 1395, 1792
AcuI CTGAAG 2 cut(s) 972, 1257
AfaI GTAC 6 cut(s) 93, 297, 655, 724, 1904, 2098
AfiI CCNNNNNNNGG 2 cut(s) 2093, 2221
AflIII ACRYGT 1 cut(s) 1946
AgeI ACCGGT 1 cut(s) 923
AgsI TTSAA 8 cut(s) 18, 463, 529, 666, 817, 1370, 2134, 2147
AhdI GACNNNNNGTC 1 cut(s) 1947
AhlI ACTAGT 1 cut(s) 1849
AjnI CCWGG 4 cut(s) 286, 1095, 1552, 1843
AjuI GAANNNNNNNTTGG 2 cut(s) 2203, 2235
AleI CACNNNNGTG 1 cut(s) 173
AloI GAACNNNNNNTCC 2 cut(s) 1212, 1244
Alw21I GWGCWC 1 cut(s) 742
Alw26I GTCTC 2 cut(s) 264, 1698
AlwI GGATC 4 cut(s) 1028, 1056, 1435, 1780
AlwNI CAGNNNCTG 1 cut(s) 1844
Ama87I CYCGRG 1 cut(s) 476
AoxI GGCC 8 cut(s) 355, 443, 472, 479, 709, 756, 1303, 1741
ApoI RAATTY 4 cut(s) 620, 1055, 1395, 1792
AsiGI ACCGGT 1 cut(s) 923
Asp700I GAANNNNTTC 1 cut(s) 1356
AspLEI GCGC 2 cut(s) 1552, 1835
AspS9I GGNCC 5 cut(s) 479, 709, 983, 1505, 1742
AsuC2I CCSGG 1 cut(s) 517
AsuHPI GGTGA 2 cut(s) 354, 1831
AsuII TTCGAA 1 cut(s) 1004
AvaI CYCGRG 1 cut(s) 476
AvaII GGWCC 2 cut(s) 983, 1505
BaeI ACNNNNGTAYC 2 cut(s) 2088, 2121
BalI TGGCCA 1 cut(s) 758
BanII GRGCYC 2 cut(s) 568, 2056
BarI GAAGNNNNNNTAC 2 cut(s) 646, 678
BauI CACGAG 1 cut(s) 2183
BbsI GAAGAC 2 cut(s) 1359, 2122
Bbv12I GWGCWC 1 cut(s) 742
BccI CCATC 8 cut(s) 199, 401, 496, 836, 905, 1712, 1753, 1925
BceAI ACGGC 2 cut(s) 1756, 2225
BcgI CGANNNNNNTGC 4 cut(s) 1755, 1789, 1847, 1881
BciT130I CCWGG 4 cut(s) 288, 1097, 1554, 1845
BcnI CCSGG 1 cut(s) 517
BcoDI GTCTC 2 cut(s) 264, 1698
BcuI ACTAGT 1 cut(s) 1849
BfaI CTAG 6 cut(s) 633, 1173, 1500, 1568, 1850, 2004
BfmI CTRYAG 2 cut(s) 672, 1209
BglI GCCNNNNNGGC 1 cut(s) 1484
Bme1390I CCNGG 5 cut(s) 288, 517, 1097, 1554, 1845
Bme18I GGWCC 2 cut(s) 983, 1505
BmeRI GACNNNNNGTC 1 cut(s) 1947
BmeT110I CYCGRG 1 cut(s) 476
BmgT120I GGNCC 5 cut(s) 479, 709, 983, 1505, 1742
BmiI GGNNCC 4 cut(s) 565, 928, 1506, 2053
BmrFI CCNGG 5 cut(s) 288, 517, 1097, 1554, 1845
BmsI GCATC 4 cut(s) 628, 883, 1321, 1690
BpiI GAAGAC 2 cut(s) 1359, 2122
BpmI CTGGAG 1 cut(s) 1680
Bpu10I CCTNAGC 1 cut(s) 945
Bpu14I TTCGAA 1 cut(s) 1004
BpuEI CTTGAG 1 cut(s) 1517
BpuMI CCSGG 1 cut(s) 517
BsaBI GATNNNNATC 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 1698
BsaJI CCNNGG 8 cut(s) 195, 475, 516, 712, 1016, 1044, 1096, 1479
BsaWI WCCGGW 2 cut(s) 923, 1410
Bsc4I CCNNNNNNNGG 2 cut(s) 2093, 2221
Bse118I RCCGGY 1 cut(s) 923
Bse1I ACTGG 2 cut(s) 55, 2200
Bse3DI GCAATG 3 cut(s) 541, 915, 1620
Bse8I GATNNNNATC 1 cut(s) 150
BseBI CCWGG 4 cut(s) 288, 1097, 1554, 1845
BseDI CCNNGG 8 cut(s) 195, 475, 516, 712, 1016, 1044, 1096, 1479
BseGI GGATG 3 cut(s) 412, 874, 1681
BseJI GATNNNNATC 1 cut(s) 150
BseLI CCNNNNNNNGG 2 cut(s) 2093, 2221
BseMI GCAATG 3 cut(s) 541, 915, 1620
BseMII CTCAG 5 cut(s) 46, 906, 2000, 2049, 2259
BseNI ACTGG 2 cut(s) 55, 2200
BseRI GAGGAG 2 cut(s) 1437, 1632
BsgI GTGCAG 1 cut(s) 1887
Bsh1236I CGCG 1 cut(s) 1550
BshFI GGCC 8 cut(s) 357, 445, 474, 481, 711, 758, 1305, 1743
BshTI ACCGGT 1 cut(s) 923
BsiHKAI GWGCWC 1 cut(s) 742
BsiHKCI CYCGRG 1 cut(s) 476
BsiSI CCGG 4 cut(s) 446, 516, 924, 1411
BslFI GGGAC 4 cut(s) 533, 1132, 1518, 1935
BslI CCNNNNNNNGG 2 cut(s) 2093, 2221
BsmAI GTCTC 2 cut(s) 264, 1698
BsmFI GGGAC 4 cut(s) 533, 1132, 1518, 1935
BsmI GAATGC 1 cut(s) 1683
BsnI GGCC 8 cut(s) 357, 445, 474, 481, 711, 758, 1305, 1743
Bso31I GGTCTC 1 cut(s) 1698
BsoBI CYCGRG 1 cut(s) 476
Bsp119I TTCGAA 1 cut(s) 1004
Bsp1286I GDGCHC 3 cut(s) 568, 742, 2056
Bsp1407I TGTACA 1 cut(s) 91
Bsp143I GATC 4 cut(s) 1020, 1048, 1440, 1772
Bsp19I CCATGG 1 cut(s) 1044
BspACI CCGC 3 cut(s) 735, 1548, 2175
BspANI GGCC 8 cut(s) 357, 445, 474, 481, 711, 758, 1305, 1743
BspCNI CTCAG 5 cut(s) 45, 905, 2001, 2050, 2260
BspFNI CGCG 1 cut(s) 1550
BspHI TCATGA 2 cut(s) 154, 2284
BspLI GGNNCC 4 cut(s) 565, 928, 1506, 2053
BspMAI CTGCAG 1 cut(s) 676
BspPI GGATC 4 cut(s) 1028, 1056, 1435, 1780
BspT104I TTCGAA 1 cut(s) 1004
BspTNI GGTCTC 1 cut(s) 1698
BsrDI GCAATG 3 cut(s) 541, 915, 1620
BsrFI RCCGGY 1 cut(s) 923
BsrGI TGTACA 1 cut(s) 91
BsrI ACTGG 2 cut(s) 55, 2200
BssAI RCCGGY 1 cut(s) 923
BssECI CCNNGG 8 cut(s) 195, 475, 516, 712, 1016, 1044, 1096, 1479
BssMI GATC 4 cut(s) 1020, 1048, 1440, 1772
BssNAI GTATAC 3 cut(s) 211, 1147, 1855
BssSI CACGAG 1 cut(s) 2183
BssT1I CCWWGG 3 cut(s) 712, 1044, 1479
Bst1107I GTATAC 3 cut(s) 211, 1147, 1855
Bst2BI CACGAG 1 cut(s) 2183
Bst2UI CCWGG 4 cut(s) 288, 1097, 1554, 1845
Bst4CI ACNGT 9 cut(s) 172, 215, 958, 1000, 1017, 1030, 2026, 2038, 2143
Bst6I CTCTTC 1 cut(s) 893
BstAUI TGTACA 1 cut(s) 91
BstBI TTCGAA 1 cut(s) 1004
BstC8I GCNNGC 4 cut(s) 165, 1039, 1466, 2238
BstDEI CTNAG 6 cut(s) 32, 892, 945, 2009, 2058, 2268
BstDSI CCRYGG 2 cut(s) 1016, 1044
BstF5I GGATG 3 cut(s) 412, 874, 1681
BstFNI CGCG 1 cut(s) 1550
BstHHI GCGC 2 cut(s) 1552, 1835
BstKTI GATC 4 cut(s) 1023, 1051, 1443, 1775
BstMAI GTCTC 2 cut(s) 264, 1698
BstMBI GATC 4 cut(s) 1020, 1048, 1440, 1772
BstMWI GCNNNNNNNGC 5 cut(s) 599, 708, 746, 755, 1484
BstNI CCWGG 4 cut(s) 288, 1097, 1554, 1845
BstNSI RCATGY 2 cut(s) 1950, 2240
BstSCI CCNGG 5 cut(s) 286, 515, 1095, 1552, 1843
BstSFI CTRYAG 2 cut(s) 672, 1209
BstUI CGCG 1 cut(s) 1550
BstV2I GAAGAC 2 cut(s) 1359, 2122
BstXI CCANNNNNNTGG 2 cut(s) 175, 495
BstZ17I GTATAC 3 cut(s) 211, 1147, 1855
BsuRI GGCC 8 cut(s) 357, 445, 474, 481, 711, 758, 1305, 1743
BtgI CCRYGG 2 cut(s) 1016, 1044
BtgZI GCGATG 1 cut(s) 1297
BtsCI GGATG 3 cut(s) 412, 874, 1681
BtsI GCAGTG 1 cut(s) 669
BtsIMutI CAGTG 3 cut(s) 168, 669, 2193
Cac8I GCNNGC 4 cut(s) 165, 1039, 1466, 2238
CaiI CAGNNNCTG 1 cut(s) 1844
CciI TCATGA 2 cut(s) 154, 2284
CfoI GCGC 2 cut(s) 1552, 1835
Cfr10I RCCGGY 1 cut(s) 923
Cfr13I GGNCC 5 cut(s) 479, 709, 983, 1505, 1742
Csp6I GTAC 6 cut(s) 92, 296, 654, 723, 1903, 2097
CspAI ACCGGT 1 cut(s) 923
CviQI GTAC 6 cut(s) 92, 296, 654, 723, 1903, 2097
DdeI CTNAG 6 cut(s) 32, 892, 945, 2009, 2058, 2268
DpnI GATC 4 cut(s) 1022, 1050, 1442, 1774
DpnII GATC 4 cut(s) 1020, 1048, 1440, 1772
DriI GACNNNNNGTC 1 cut(s) 1947
EaeI YGGCCR 2 cut(s) 443, 756
Eam1104I CTCTTC 1 cut(s) 893
Eam1105I GACNNNNNGTC 1 cut(s) 1947
EarI CTCTTC 1 cut(s) 893
Eco130I CCWWGG 3 cut(s) 712, 1044, 1479
Eco147I AGGCCT 1 cut(s) 474
Eco24I GRGCYC 2 cut(s) 568, 2056
Eco31I GGTCTC 1 cut(s) 1698
Eco32I GATATC 1 cut(s) 1424
Eco47I GGWCC 2 cut(s) 983, 1505
Eco57I CTGAAG 2 cut(s) 972, 1257
Eco88I CYCGRG 1 cut(s) 476
EcoO109I RGGNCCY 1 cut(s) 709
EcoRI GAATTC 2 cut(s) 620, 1395
EcoRII CCWGG 4 cut(s) 286, 1095, 1552, 1843
EcoRV GATATC 1 cut(s) 1424
EcoT14I CCWWGG 3 cut(s) 712, 1044, 1479
EcoT22I ATGCAT 2 cut(s) 1367, 1683
EcoT38I GRGCYC 2 cut(s) 568, 2056
ErhI CCWWGG 3 cut(s) 712, 1044, 1479
FalI AAGNNNNNCTT 4 cut(s) 1318, 1350, 2203, 2235
FaqI GGGAC 4 cut(s) 533, 1132, 1518, 1935
FblI GTMKAC 4 cut(s) 210, 1146, 1415, 1854
FokI GGATG 3 cut(s) 419, 861, 1668
FriOI GRGCYC 2 cut(s) 568, 2056
FspBI CTAG 6 cut(s) 633, 1173, 1500, 1568, 1850, 2004
GlaI GCGC 2 cut(s) 1551, 1834
GsuI CTGGAG 1 cut(s) 1680
HaeIII GGCC 8 cut(s) 357, 445, 474, 481, 711, 758, 1305, 1743
HapII CCGG 4 cut(s) 446, 516, 924, 1411
HhaI GCGC 2 cut(s) 1552, 1835
Hin6I GCGC 2 cut(s) 1550, 1833
HinP1I GCGC 2 cut(s) 1550, 1833
HincII GTYRAC 4 cut(s) 1375, 1638, 1981, 2227
HindII GTYRAC 4 cut(s) 1375, 1638, 1981, 2227
HindIII AAGCTT 2 cut(s) 819, 1138
HpaI GTTAAC 1 cut(s) 1981
HpaII CCGG 4 cut(s) 446, 516, 924, 1411
HphI GGTGA 2 cut(s) 354, 1831
Hpy188I TCNGA 8 cut(s) 199, 280, 339, 454, 563, 895, 1808, 2010
Hpy188III TCNNGA 7 cut(s) 155, 428, 1052, 1623, 1672, 1923, 2285
Hpy99I CGWCG 1 cut(s) 1257
HpyAV CCTTC 5 cut(s) 326, 384, 996, 1763, 1998
HpyCH4III ACNGT 9 cut(s) 172, 215, 958, 1000, 1017, 1030, 2026, 2038, 2143
HpyCH4IV ACGT 1 cut(s) 1977
HpyF10VI GCNNNNNNNGC 5 cut(s) 599, 708, 746, 755, 1484
HpyF3I CTNAG 6 cut(s) 32, 892, 945, 2009, 2058, 2268
HpySE526I ACGT 1 cut(s) 1977
HspAI GCGC 2 cut(s) 1550, 1833
KspAI GTTAAC 1 cut(s) 1981
Kzo9I GATC 4 cut(s) 1020, 1048, 1440, 1772
LmnI GCTCC 4 cut(s) 563, 745, 968, 2051
LweI GCATC 4 cut(s) 628, 883, 1321, 1690
MaeI CTAG 6 cut(s) 633, 1173, 1500, 1568, 1850, 2004
MaeII ACGT 1 cut(s) 1977
MaeIII GTNAC 7 cut(s) 215, 1648, 1720, 1993, 2020, 2038, 2198
MalI GATC 4 cut(s) 1022, 1050, 1442, 1774
MboI GATC 4 cut(s) 1020, 1048, 1440, 1772
MboII GAAGA 5 cut(s) 377, 880, 1364, 1969, 2122
MfeI CAATTG 3 cut(s) 867, 1455, 2100
MhlI GDGCHC 3 cut(s) 568, 742, 2056
MlsI TGGCCA 1 cut(s) 758
MluNI TGGCCA 1 cut(s) 758
MlyI GAGTC 4 cut(s) 266, 458, 1163, 2069
MmeI TCCRAC 3 cut(s) 755, 1198, 2029
Mox20I TGGCCA 1 cut(s) 758
Mph1103I ATGCAT 2 cut(s) 1367, 1683
MroXI GAANNNNTTC 1 cut(s) 1356
MscI TGGCCA 1 cut(s) 758
MseI TTAA 3 cut(s) 137, 1200, 1980
MslI CAYNNNNRTG 5 cut(s) 173, 498, 648, 1244, 2289
Msp20I TGGCCA 1 cut(s) 758
MspA1I CMGCKG 2 cut(s) 677, 806
MspI CCGG 4 cut(s) 446, 516, 924, 1411
MspR9I CCNGG 5 cut(s) 288, 517, 1097, 1554, 1845
MunI CAATTG 3 cut(s) 867, 1455, 2100
Mva1269I GAATGC 1 cut(s) 1683
MvaI CCWGG 4 cut(s) 288, 1097, 1554, 1845
MvnI CGCG 1 cut(s) 1550
MwoI GCNNNNNNNGC 5 cut(s) 599, 708, 746, 755, 1484
NciI CCSGG 1 cut(s) 517
NcoI CCATGG 1 cut(s) 1044
NdeII GATC 4 cut(s) 1020, 1048, 1440, 1772
NlaIV GGNNCC 4 cut(s) 565, 928, 1506, 2053
NmeAIII GCCGAG 1 cut(s) 619
NmuCI GTSAC 3 cut(s) 1648, 1993, 2020
NsiI ATGCAT 2 cut(s) 1367, 1683
NspI RCATGY 2 cut(s) 1950, 2240
NspV TTCGAA 1 cut(s) 1004
OliI CACNNNNGTG 1 cut(s) 173
PaeI GCATGC 1 cut(s) 2240
PagI TCATGA 2 cut(s) 154, 2284
PceI AGGCCT 1 cut(s) 474
PciI ACATGT 1 cut(s) 1946
PctI GAATGC 1 cut(s) 1683
PdmI GAANNNNTTC 1 cut(s) 1356
PfeI GAWTC 8 cut(s) 146, 300, 455, 512, 1187, 1321, 1822, 2248
PinAI ACCGGT 1 cut(s) 923
PleI GAGTC 4 cut(s) 265, 457, 1162, 2068
PpsI GAGTC 4 cut(s) 265, 457, 1162, 2068
PscI ACATGT 1 cut(s) 1946
Psp6I CCWGG 4 cut(s) 286, 1095, 1552, 1843
PspGI CCWGG 4 cut(s) 286, 1095, 1552, 1843
PspN4I GGNNCC 4 cut(s) 565, 928, 1506, 2053
PspPI GGNCC 5 cut(s) 479, 709, 983, 1505, 1742
PsrI GAACNNNNNNTAC 2 cut(s) 2183, 2215
PstI CTGCAG 1 cut(s) 676
PstNI CAGNNNCTG 1 cut(s) 1844
PvuII CAGCTG 2 cut(s) 677, 806
RsaI GTAC 6 cut(s) 93, 297, 655, 724, 1904, 2098
RsaNI GTAC 6 cut(s) 92, 296, 654, 723, 1903, 2097
RseI CAYNNNNRTG 5 cut(s) 173, 498, 648, 1244, 2289
SaqAI TTAA 3 cut(s) 137, 1200, 1980
Sau3AI GATC 4 cut(s) 1020, 1048, 1440, 1772
Sau96I GGNCC 5 cut(s) 479, 709, 983, 1505, 1742
SchI GAGTC 4 cut(s) 266, 458, 1163, 2069
ScrFI CCNGG 5 cut(s) 288, 517, 1097, 1554, 1845
SduI GDGCHC 3 cut(s) 568, 742, 2056
SfaNI GCATC 4 cut(s) 628, 883, 1321, 1690
SfcI CTRYAG 2 cut(s) 672, 1209
SfuI TTCGAA 1 cut(s) 1004
SinI GGWCC 2 cut(s) 983, 1505
SmiMI CAYNNNNRTG 5 cut(s) 173, 498, 648, 1244, 2289
SmlI CTYRAG 2 cut(s) 10, 1532
SmoI CTYRAG 2 cut(s) 10, 1532
SpeI ACTAGT 1 cut(s) 1849
SphI GCATGC 1 cut(s) 2240
SseBI AGGCCT 1 cut(s) 474
SsiI CCGC 3 cut(s) 735, 1548, 2175
SspI AATATT 1 cut(s) 382
SspMI CTAG 6 cut(s) 633, 1173, 1500, 1568, 1850, 2004
StuI AGGCCT 1 cut(s) 474
StyD4I CCNGG 5 cut(s) 286, 515, 1095, 1552, 1843
StyI CCWWGG 3 cut(s) 712, 1044, 1479
TaaI ACNGT 9 cut(s) 172, 215, 958, 1000, 1017, 1030, 2026, 2038, 2143
TaiI ACGT 1 cut(s) 1980
TaqI TCGA 4 cut(s) 1004, 1051, 1393, 1924
TatI WGTACW 1 cut(s) 91
TauI GCSGC 1 cut(s) 1550
TfiI GAWTC 8 cut(s) 146, 300, 455, 512, 1187, 1321, 1822, 2248
Tru1I TTAA 3 cut(s) 137, 1200, 1980
Tru9I TTAA 3 cut(s) 137, 1200, 1980
TscAI CASTG 3 cut(s) 175, 676, 2200
TseFI GTSAC 3 cut(s) 1648, 1993, 2020
Tsp45I GTSAC 3 cut(s) 1648, 1993, 2020
TspDTI ATGAA 8 cut(s) 308, 447, 480, 923, 1365, 1581, 1785, 2204
TspGWI ACGGA 3 cut(s) 269, 1981, 2244
TspRI CASTG 3 cut(s) 175, 676, 2200
VpaK11BI GGWCC 2 cut(s) 983, 1505
XapI RAATTY 4 cut(s) 620, 1055, 1395, 1792
XceI RCATGY 2 cut(s) 1950, 2240
XcmI CCANNNNNNNNNTGG 1 cut(s) 397
XmiI GTMKAC 4 cut(s) 210, 1146, 1415, 1854
XmnI GAANNNNTTC 1 cut(s) 1356
XspI CTAG 6 cut(s) 633, 1173, 1500, 1568, 1850, 2004
Zsp2I ATGCAT 2 cut(s) 1367, 1683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.