FvH4_5g10490

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001127
Physical Location & Seq
Reverse (-)
5963807 .. 5969111
5305 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g10490.t1

Sequence Viewer

Length: 2355 bp
ATGGGATCTCTTCTAATGAAGAAGAAGAGCCAAAAAGTGTCACTTTTTGTGACATTCAATCTTTTCTTCATCCTCTGTAGCCAAAACAGCATCATTAGGACTGTGGATGCCAAGAGTAAAGTTCACATAGTGTATATGGGTGAAAAGCACCACCATGATCCTGAGGTTGTAACCTGTTTGCATCATGACATGCTTGCCTCTGTTGTGGGGAGCAAAGAAGCAGCCACTGATGCTATGGTGTACAGTTACAAACATGGATTTTCTGGTTTTGCAGCTAAATTTACAGAATCTCAAGTGAAGAAGATTGCAGATTTTCCTGGAGTTATCCGCGTTATACCAAATCAGTTTCATTCACTGCAAACAACTAGGAGCTGGGATTATCTAGGGCTCTCCCCTAATTCTCCTAAAAACCTTTTGAATGACACAAACTTGGGTGATGGAATTATCATTGGTCTACTGGACACTGGTATACGGCCAGAGTCAGAAGTATTCAATGATGAGGATCTGGGGCCAATCCCAAGTCAATGGAAGGGTCAATGTGTCTCTGGACAACAATTCAATGCCTCAACAGCTTGTAACAATAAGCTAATAGGAGCAAAATATTACATTGATGGGTTTCTTGCTGAAAATCAACAACCATTCAACACTACAGACAGCCCGGACTACATGTCCCCAAGAGATGTTGTTGGACATGGAACACATACATCTACAATTGCTGGTGGCTCATTTGTGTACAATGCAAGCTACAAAGGTATTGGCCTTGGAATAGTCAGAGGTGGTGCTCCTCGTGCTCGTTTGGCTATGTACAAAGTGTGCTGGAATGTGCCAAGAGGACAGTGCTCAAATGCTGACTTACTGAAAGCTTTTGATGATGCAATTCATGATGGAGTTGATGTCATATCAGTATCTCTTGGCACTCAACTTCCACTGTTTTCAGAGGTTGATGACCGCGATGCAATCTCCATTGGTTCATTCCATGCTGTTACTAAAGGCATACCTGTTGTTTGCGCAGCTGCAAATGAAGGACCTTCTGCATACACTGTCGAAAACACAGCACCTTGGATCTTAACTGTAGCAGCTTCTACTATTGATCGGTCCTTTCCTACAAATATTACACTAGGGAACAACTTAACCATACTGGGTCAAGCCTTGTTTGCAGGAACAGAAGTTGACTTTACTGGTTTGGTGTACCCAGAGAACCCAGGACTTATTCCTTCACTAGCTGGTGTTTGTGAGGCTCTATTACTCAATAACACACCGGTGGCTGGAAATGTAGTCCTTTGCTTTACATCAGTAGCCAGACGAACTCCGGTAGCACTTGCTGTCTCTAGTGTCAAAGCAGCTGGTGGGGTTGGAGTAATTGTTGCCAAGAGTCCTGGTGATGTCTTGGGTCCTTGTAGCAGTGACTTCCCATGCATTGAAGTAGACTACGAGCTTGGTACTCAGATACTATTATACATTCGCTCCACCAGTTCTCCTGTGGTAAAACTGAATCCTTCTGTGACACTGGTGGGGAAGCCTGTATCAACCAAGGTTGCCGCTTTCTCATCTAGGGGGCCTAACTCCATTTCTCCAGCCATACTAAAGCCGGACATAGCTGCACCTGGTGTGAGTATACTAGCTGCCAGTGCGCCTTTTGATCCATACATGAACGGAGGATTTGCCTTGCATTCAGGAACATCAATGGCAACTCCTCATGTTTCGGGGATTGTGGCGCTTCTAAAAGCATTACACTCCAATTGGTCACCTGCTGCAATTAGATCAGCAATAGTAACAACAGCATGGAGGACTGATCCATTCGGAGAGCCAATCTTTGCTGAGGGGTCACCGCAAAAGCTTGCTGATCCATTTGATTATGGAGGTGGAATTGTGAACCCGAACAAAGCTGCAGACCCTGGTCTAGTATATGACCTGGGCGTATATGACTACATACTTTACCTATGTGCTGTTGGGTACAACAACTCAGAAATTTCACAACTTGTTGGGAATTCAACAACATGTTCTAGTACAAAGCCTTCTGTTCTTGATGTGAACCTGCCATCCATTACCGTTCCAAATCTAAGAGAGAACATTACACTCACCAGAAGTGTCACAAATGTCGGTCCAGTCAATTCGACATACAAAGCTCGGATAAGCCCTCCGTGGGGAATCAGTGTGGCTGTGAGCCCTGAGACCTTGGTTTTCAACTCGAACATTGAAACAATCTATTTCACAGTTGAGGTTTCAACCACCCATGAAGTGAACACAGGGTACTACTTCGGCAGCCTCGCTTGGACTGATGAATGGGGGCATGTGGTGACAATTCCCATGTCTGTGAGGACACAAATCATTCTATATTATGCAGATGGCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

785

Amino Acids

83.51

Weight (kDa)

5.79

Isoelectric Point (pI)

33.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 41 - 119 1.8e-19 Peptidase inhibitor I9
Peptidase_S8 PF00082 145 - 606 1.5e-45 Subtilase family
fn3_6 PF17766 676 - 772 1.1e-25 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1756
Acc16I TGCGCA 1 cut(s) 1009
Acc36I ACCTGC 2 cut(s) 1756, 2043
AccI GTMKAC 4 cut(s) 454, 469, 1425, 1615
AccII CGCG 2 cut(s) 330, 951
AciI CCGC 4 cut(s) 328, 949, 1539, 1829
AclWI GGATC 7 cut(s) 13, 152, 510, 1070, 1634, 1787, 1838
AcoI YGGCCR 1 cut(s) 473
AcsI RAATTY 3 cut(s) 278, 1968, 1987
AdeI CACNNNGTG 2 cut(s) 130, 1607
AfaI GTAC 8 cut(s) 242, 734, 806, 1190, 1441, 1955, 2008, 2252
AfiI CCNNNNNNNGG 3 cut(s) 1265, 2143, 2144
AflIII ACRYGT 2 cut(s) 666, 1997
AgeI ACCGGT 1 cut(s) 1258
AhdI GACNNNNNGTC 1 cut(s) 667
AjnI CCWGG 6 cut(s) 316, 1201, 1375, 1603, 1894, 1911
AleI CACNNNNGTG 1 cut(s) 1259
AloI GAACNNNNNNTCC 2 cut(s) 2024, 2056
Alw21I GWGCWC 3 cut(s) 784, 793, 842
Alw26I GTCTC 3 cut(s) 547, 1330, 2165
AlwI GGATC 7 cut(s) 13, 152, 510, 1070, 1634, 1787, 1838
AlwNI CAGNNNCTG 2 cut(s) 227, 1895
AoxI GGCC 4 cut(s) 473, 509, 757, 1556
ApoI RAATTY 3 cut(s) 278, 1968, 1987
AsiGI ACCGGT 1 cut(s) 1258
AspLEI GCGC 3 cut(s) 1010, 1633, 1717
AspS9I GGNCC 6 cut(s) 509, 1025, 1095, 1391, 1556, 2102
AsuC2I CCSGG 1 cut(s) 659
AsuHPI GGTGA 7 cut(s) 152, 446, 1391, 1737, 1818, 2071, 2308
AvaII GGWCC 4 cut(s) 1025, 1095, 1391, 2102
AxyI CCTNAGG 1 cut(s) 162
BanII GRGCYC 2 cut(s) 390, 2168
BarI GAAGNNNNNNTAC 2 cut(s) 1999, 2031
BauI CACGAG 1 cut(s) 786
Bbv12I GWGCWC 3 cut(s) 784, 793, 842
BbvCI CCTCAGC 1 cut(s) 1818
BccI CCATC 5 cut(s) 431, 605, 878, 2047, 2339
BceAI ACGGC 1 cut(s) 488
BciT130I CCWGG 6 cut(s) 318, 1203, 1377, 1605, 1896, 1913
BcnI CCSGG 1 cut(s) 659
BcoDI GTCTC 3 cut(s) 547, 1330, 2165
BfaI CTAG 9 cut(s) 366, 383, 1118, 1220, 1329, 1551, 1619, 1901, 2004
BfmI CTRYAG 4 cut(s) 76, 648, 1071, 1887
BfoI RGCGCY 1 cut(s) 1718
BfuAI ACCTGC 2 cut(s) 1756, 2043
Bme1390I CCNGG 7 cut(s) 318, 659, 1203, 1377, 1605, 1896, 1913
Bme18I GGWCC 4 cut(s) 1025, 1095, 1391, 2102
BmeRI GACNNNNNGTC 1 cut(s) 667
BmgT120I GGNCC 6 cut(s) 509, 1025, 1095, 1391, 1556, 2102
BmiI GGNNCC 3 cut(s) 510, 1392, 1557
BmrFI CCNGG 7 cut(s) 318, 659, 1203, 1377, 1605, 1896, 1913
BmrI ACTGGG 1 cut(s) 1148
BmsI GCATC 6 cut(s) 97, 99, 190, 220, 862, 943
BmuI ACTGGG 1 cut(s) 1148
BoxI GACNNNNGTC 1 cut(s) 1896
BpmI CTGGAG 2 cut(s) 339, 1557
Bpu10I CCTNAGC 1 cut(s) 1818
BpuEI CTTGAG 1 cut(s) 276
BpuMI CCSGG 1 cut(s) 659
BsaBI GATNNNNATC 1 cut(s) 501
BsaI GGTCTC 1 cut(s) 2165
BsaJI CCNNGG 8 cut(s) 760, 1058, 1201, 1530, 1894, 1912, 2141, 2175
BsaWI WCCGGW 2 cut(s) 1258, 1309
BsaXI ACNNNNNCTCC 8 cut(s) 879, 909, 1347, 1377, 1448, 1459, 1478, 1489
Bsc4I CCNNNNNNNGG 3 cut(s) 1265, 2143, 2144
Bse118I RCCGGY 1 cut(s) 1258
Bse1I ACTGG 8 cut(s) 462, 469, 1143, 1183, 1470, 1512, 1626, 2105
Bse21I CCTNAGG 1 cut(s) 162
Bse8I GATNNNNATC 1 cut(s) 501
BseBI CCWGG 6 cut(s) 318, 1203, 1377, 1605, 1896, 1913
BseDI CCNNGG 8 cut(s) 760, 1058, 1201, 1530, 1894, 1912, 2141, 2175
BseGI GGATG 3 cut(s) 69, 112, 2039
BseJI GATNNNNATC 1 cut(s) 501
BseLI CCNNNNNNNGG 3 cut(s) 1265, 2143, 2144
BseMII CTCAG 5 cut(s) 153, 1457, 1809, 1977, 2160
BseNI ACTGG 8 cut(s) 462, 469, 1143, 1183, 1470, 1512, 1626, 2105
BseRI GAGGAG 2 cut(s) 774, 1683
BseYI CCCAGC 1 cut(s) 372
BsgI GTGCAG 1 cut(s) 1584
Bsh1236I CGCG 2 cut(s) 330, 951
BshFI GGCC 4 cut(s) 475, 511, 759, 1558
BshTI ACCGGT 1 cut(s) 1258
BsiHKAI GWGCWC 3 cut(s) 784, 793, 842
BsiSI CCGG 4 cut(s) 659, 1259, 1310, 1589
BslFI GGGAC 1 cut(s) 655
BslI CCNNNNNNNGG 3 cut(s) 1265, 2143, 2144
BsmAI GTCTC 3 cut(s) 547, 1330, 2165
BsmFI GGGAC 1 cut(s) 655
BsmI GAATGC 1 cut(s) 1669
BsnI GGCC 4 cut(s) 475, 511, 759, 1558
Bso31I GGTCTC 1 cut(s) 2165
Bsp1286I GDGCHC 5 cut(s) 390, 784, 793, 842, 2168
Bsp1407I TGTACA 3 cut(s) 240, 732, 804
Bsp143I GATC 9 cut(s) 5, 157, 502, 1062, 1090, 1639, 1760, 1792, 1843
BspACI CCGC 4 cut(s) 328, 949, 1539, 1829
BspANI GGCC 4 cut(s) 475, 511, 759, 1558
BspCNI CTCAG 5 cut(s) 154, 1456, 1810, 1976, 2161
BspFNI CGCG 2 cut(s) 330, 951
BspHI TCATGA 2 cut(s) 184, 880
BspLI GGNNCC 3 cut(s) 510, 1392, 1557
BspMAI CTGCAG 1 cut(s) 1891
BspMI ACCTGC 2 cut(s) 1756, 2043
BspPI GGATC 7 cut(s) 13, 152, 510, 1070, 1634, 1787, 1838
BspQI GCTCTTC 1 cut(s) 20
BspTNI GGTCTC 1 cut(s) 2165
BsrFI RCCGGY 1 cut(s) 1258
BsrGI TGTACA 3 cut(s) 240, 732, 804
BsrI ACTGG 8 cut(s) 462, 469, 1143, 1183, 1470, 1512, 1626, 2105
BssAI RCCGGY 1 cut(s) 1258
BssECI CCNNGG 8 cut(s) 760, 1058, 1201, 1530, 1894, 1912, 2141, 2175
BssMI GATC 9 cut(s) 5, 157, 502, 1062, 1090, 1639, 1760, 1792, 1843
BssNAI GTATAC 2 cut(s) 470, 1616
BssSI CACGAG 1 cut(s) 786
BssT1I CCWWGG 4 cut(s) 760, 1058, 1530, 2175
Bst1107I GTATAC 2 cut(s) 470, 1616
Bst2BI CACGAG 1 cut(s) 786
Bst2UI CCWGG 6 cut(s) 318, 1203, 1377, 1605, 1896, 1913
Bst4CI ACNGT 8 cut(s) 103, 245, 837, 930, 1042, 1072, 2050, 2215
Bst6I CTCTTC 2 cut(s) 15, 20
BstAUI TGTACA 3 cut(s) 240, 732, 804
BstC8I GCNNGC 3 cut(s) 195, 742, 1839
BstDEI CTNAG 6 cut(s) 162, 1443, 1818, 1963, 2060, 2169
BstDSI CCRYGG 1 cut(s) 2141
BstEII GGTNACC 2 cut(s) 1743, 1824
BstF5I GGATG 3 cut(s) 69, 112, 2039
BstFNI CGCG 2 cut(s) 330, 951
BstH2I RGCGCY 1 cut(s) 1718
BstHHI GCGC 3 cut(s) 1010, 1633, 1717
BstKTI GATC 9 cut(s) 8, 160, 505, 1065, 1093, 1642, 1763, 1795, 1846
BstMAI GTCTC 3 cut(s) 547, 1330, 2165
BstMBI GATC 9 cut(s) 5, 157, 502, 1062, 1090, 1639, 1760, 1792, 1843
BstMWI GCNNNNNNNGC 7 cut(s) 87, 230, 569, 788, 797, 1154, 1628
BstNI CCWGG 6 cut(s) 318, 1203, 1377, 1605, 1896, 1913
BstNSI RCATGY 4 cut(s) 193, 670, 2001, 2294
BstPAI GACNNNNGTC 1 cut(s) 1896
BstPI GGTNACC 2 cut(s) 1743, 1824
BstSCI CCNGG 7 cut(s) 316, 657, 1201, 1375, 1603, 1894, 1911
BstSFI CTRYAG 4 cut(s) 76, 648, 1071, 1887
BstUI CGCG 2 cut(s) 330, 951
BstX2I RGATCY 3 cut(s) 5, 502, 1062
BstXI CCANNNNNNTGG 1 cut(s) 525
BstYI RGATCY 3 cut(s) 5, 502, 1062
BstZ17I GTATAC 2 cut(s) 470, 1616
Bsu36I CCTNAGG 1 cut(s) 162
BsuRI GGCC 4 cut(s) 475, 511, 759, 1558
BtgI CCRYGG 1 cut(s) 2141
BtgZI GCGATG 1 cut(s) 966
BtsCI GGATG 3 cut(s) 69, 112, 2039
BtsI GCAGTG 2 cut(s) 353, 1408
BveI ACCTGC 2 cut(s) 1756, 2043
Cac8I GCNNGC 3 cut(s) 195, 742, 1839
CaiI CAGNNNCTG 2 cut(s) 227, 1895
CciI TCATGA 2 cut(s) 184, 880
CfoI GCGC 3 cut(s) 1010, 1633, 1717
Cfr10I RCCGGY 1 cut(s) 1258
Cfr13I GGNCC 6 cut(s) 509, 1025, 1095, 1391, 1556, 2102
CsiI ACCWGGT 1 cut(s) 1603
Csp6I GTAC 8 cut(s) 241, 733, 805, 1189, 1440, 1954, 2007, 2251
CspAI ACCGGT 1 cut(s) 1258
CviQI GTAC 8 cut(s) 241, 733, 805, 1189, 1440, 1954, 2007, 2251
DdeI CTNAG 6 cut(s) 162, 1443, 1818, 1963, 2060, 2169
DpnI GATC 9 cut(s) 7, 159, 504, 1064, 1092, 1641, 1762, 1794, 1845
DpnII GATC 9 cut(s) 5, 157, 502, 1062, 1090, 1639, 1760, 1792, 1843
DraIII CACNNNGTG 2 cut(s) 130, 1607
DriI GACNNNNNGTC 1 cut(s) 667
EaeI YGGCCR 1 cut(s) 473
Eam1104I CTCTTC 2 cut(s) 15, 20
Eam1105I GACNNNNNGTC 1 cut(s) 667
EarI CTCTTC 2 cut(s) 15, 20
Eco130I CCWWGG 4 cut(s) 760, 1058, 1530, 2175
Eco24I GRGCYC 2 cut(s) 390, 2168
Eco31I GGTCTC 1 cut(s) 2165
Eco47I GGWCC 4 cut(s) 1025, 1095, 1391, 2102
Eco81I CCTNAGG 1 cut(s) 162
Eco91I GGTNACC 2 cut(s) 1743, 1824
EcoO109I RGGNCCY 3 cut(s) 1025, 1391, 1556
EcoO65I GGTNACC 2 cut(s) 1743, 1824
EcoRI GAATTC 1 cut(s) 1987
EcoRII CCWGG 6 cut(s) 316, 1201, 1375, 1603, 1894, 1911
EcoT14I CCWWGG 4 cut(s) 760, 1058, 1530, 2175
EcoT22I ATGCAT 1 cut(s) 1418
EcoT38I GRGCYC 2 cut(s) 390, 2168
ErhI CCWWGG 4 cut(s) 760, 1058, 1530, 2175
FalI AAGNNNNNCTT 2 cut(s) 27, 59
FaqI GGGAC 1 cut(s) 655
FblI GTMKAC 4 cut(s) 454, 469, 1425, 1615
FokI GGATG 3 cut(s) 56, 119, 2026
FriOI GRGCYC 2 cut(s) 390, 2168
FspBI CTAG 9 cut(s) 366, 383, 1118, 1220, 1329, 1551, 1619, 1901, 2004
FspI TGCGCA 1 cut(s) 1009
GlaI GCGC 3 cut(s) 1009, 1632, 1716
GsaI CCCAGC 1 cut(s) 376
GsuI CTGGAG 2 cut(s) 339, 1557
HaeII RGCGCY 1 cut(s) 1718
HaeIII GGCC 4 cut(s) 475, 511, 759, 1558
HapII CCGG 4 cut(s) 659, 1259, 1310, 1589
HhaI GCGC 3 cut(s) 1010, 1633, 1717
Hin6I GCGC 3 cut(s) 1008, 1631, 1715
HinP1I GCGC 3 cut(s) 1008, 1631, 1715
HincII GTYRAC 1 cut(s) 1171
HindII GTYRAC 1 cut(s) 1171
HindIII AAGCTT 2 cut(s) 861, 1835
HinfI GANTC 5 cut(s) 287, 479, 1372, 1492, 2148
HpaII CCGG 4 cut(s) 659, 1259, 1310, 1589
HphI GGTGA 7 cut(s) 152, 446, 1391, 1737, 1818, 2071, 2308
Hpy188I TCNGA 7 cut(s) 484, 773, 937, 1446, 1802, 1966, 2130
Hpy188III TCNNGA 6 cut(s) 161, 185, 546, 881, 1674, 2024
HpyAV CCTTC 6 cut(s) 523, 1016, 1038, 1224, 1506, 2025
HpyCH4III ACNGT 8 cut(s) 103, 245, 837, 930, 1042, 1072, 2050, 2215
HpyF10VI GCNNNNNNNGC 7 cut(s) 87, 230, 569, 788, 797, 1154, 1628
HpyF3I CTNAG 6 cut(s) 162, 1443, 1818, 1963, 2060, 2169
HspAI GCGC 3 cut(s) 1008, 1631, 1715
Kzo9I GATC 9 cut(s) 5, 157, 502, 1062, 1090, 1639, 1760, 1792, 1843
LguI GCTCTTC 1 cut(s) 20
LmnI GCTCC 5 cut(s) 210, 369, 593, 787, 1469
LweI GCATC 6 cut(s) 97, 99, 190, 220, 862, 943
MabI ACCWGGT 1 cut(s) 1603
MaeI CTAG 9 cut(s) 366, 383, 1118, 1220, 1329, 1551, 1619, 1901, 2004
MalI GATC 9 cut(s) 7, 159, 504, 1064, 1092, 1641, 1762, 1794, 1845
MboI GATC 9 cut(s) 5, 157, 502, 1062, 1090, 1639, 1760, 1792, 1843
MboII GAAGA 6 cut(s) 31, 34, 37, 58, 310, 313
MfeI CAATTG 2 cut(s) 711, 1738
MflI RGATCY 3 cut(s) 5, 502, 1062
MhlI GDGCHC 5 cut(s) 390, 784, 793, 842, 2168
MlyI GAGTC 2 cut(s) 488, 1381
MmeI TCCRAC 2 cut(s) 667, 1333
Mph1103I ATGCAT 1 cut(s) 1418
MseI TTAA 2 cut(s) 1067, 1130
MslI CAYNNNNRTG 3 cut(s) 153, 1259, 2312
MspA1I CMGCKG 2 cut(s) 1013, 1343
MspI CCGG 4 cut(s) 659, 1259, 1310, 1589
MspR9I CCNGG 7 cut(s) 318, 659, 1203, 1377, 1605, 1896, 1913
MunI CAATTG 2 cut(s) 711, 1738
Mva1269I GAATGC 1 cut(s) 1669
MvaI CCWGG 6 cut(s) 318, 1203, 1377, 1605, 1896, 1913
MvnI CGCG 2 cut(s) 330, 951
MwoI GCNNNNNNNGC 7 cut(s) 87, 230, 569, 788, 797, 1154, 1628
NciI CCSGG 1 cut(s) 659
NdeII GATC 9 cut(s) 5, 157, 502, 1062, 1090, 1639, 1760, 1792, 1843
NlaIV GGNNCC 3 cut(s) 510, 1392, 1557
NmuCI GTSAC 8 cut(s) 39, 49, 1403, 1501, 1743, 1824, 2089, 2296
NsbI TGCGCA 1 cut(s) 1009
NsiI ATGCAT 1 cut(s) 1418
NspI RCATGY 4 cut(s) 193, 670, 2001, 2294
OliI CACNNNNGTG 1 cut(s) 1259
PagI TCATGA 2 cut(s) 184, 880
PaqCI CACCTGC 1 cut(s) 1756
PciI ACATGT 2 cut(s) 666, 1997
PciSI GCTCTTC 1 cut(s) 20
PctI GAATGC 1 cut(s) 1669
PfeI GAWTC 3 cut(s) 287, 1492, 2148
PfoI TCCNGGA 1 cut(s) 316
PinAI ACCGGT 1 cut(s) 1258
PleI GAGTC 2 cut(s) 487, 1380
PpsI GAGTC 2 cut(s) 487, 1380
PpuMI RGGWCCY 2 cut(s) 1025, 1391
PscI ACATGT 2 cut(s) 666, 1997
PshAI GACNNNNGTC 1 cut(s) 1896
Psp5II RGGWCCY 2 cut(s) 1025, 1391
Psp6I CCWGG 6 cut(s) 316, 1201, 1375, 1603, 1894, 1911
PspEI GGTNACC 2 cut(s) 1743, 1824
PspFI CCCAGC 1 cut(s) 372
PspGI CCWGG 6 cut(s) 316, 1201, 1375, 1603, 1894, 1911
PspN4I GGNNCC 3 cut(s) 510, 1392, 1557
PspPI GGNCC 6 cut(s) 509, 1025, 1095, 1391, 1556, 2102
PspPPI RGGWCCY 2 cut(s) 1025, 1391
PsrI GAACNNNNNNTAC 2 cut(s) 2234, 2266
PstI CTGCAG 1 cut(s) 1891
PstNI CAGNNNCTG 2 cut(s) 227, 1895
PsuI RGATCY 3 cut(s) 5, 502, 1062
PvuII CAGCTG 2 cut(s) 1013, 1343
RsaI GTAC 8 cut(s) 242, 734, 806, 1190, 1441, 1955, 2008, 2252
RsaNI GTAC 8 cut(s) 241, 733, 805, 1189, 1440, 1954, 2007, 2251
RseI CAYNNNNRTG 3 cut(s) 153, 1259, 2312
SapI GCTCTTC 1 cut(s) 20
SaqAI TTAA 2 cut(s) 1067, 1130
Sau3AI GATC 9 cut(s) 5, 157, 502, 1062, 1090, 1639, 1760, 1792, 1843
Sau96I GGNCC 6 cut(s) 509, 1025, 1095, 1391, 1556, 2102
SchI GAGTC 2 cut(s) 488, 1381
ScrFI CCNGG 7 cut(s) 318, 659, 1203, 1377, 1605, 1896, 1913
SduI GDGCHC 5 cut(s) 390, 784, 793, 842, 2168
SexAI ACCWGGT 1 cut(s) 1603
SfaNI GCATC 6 cut(s) 97, 99, 190, 220, 862, 943
SfcI CTRYAG 4 cut(s) 76, 648, 1071, 1887
SgrAI CRCCGGYG 1 cut(s) 1258
SinI GGWCC 4 cut(s) 1025, 1095, 1391, 2102
SmiMI CAYNNNNRTG 3 cut(s) 153, 1259, 2312
SmlI CTYRAG 1 cut(s) 291
SmoI CTYRAG 1 cut(s) 291
SsiI CCGC 4 cut(s) 328, 949, 1539, 1829
SspI AATATT 2 cut(s) 602, 1111
SspMI CTAG 9 cut(s) 366, 383, 1118, 1220, 1329, 1551, 1619, 1901, 2004
StyD4I CCNGG 7 cut(s) 316, 657, 1201, 1375, 1603, 1894, 1911
StyI CCWWGG 4 cut(s) 760, 1058, 1530, 2175
TaaI ACNGT 8 cut(s) 103, 245, 837, 930, 1042, 1072, 2050, 2215
TaqI TCGA 3 cut(s) 1044, 2114, 2189
TaqII GACCGA 2 cut(s) 1083, 2090
TatI WGTACW 4 cut(s) 240, 732, 804, 2006
TauI GCSGC 1 cut(s) 1541
TfiI GAWTC 3 cut(s) 287, 1492, 2148
Tru1I TTAA 2 cut(s) 1067, 1130
Tru9I TTAA 2 cut(s) 1067, 1130
TseFI GTSAC 8 cut(s) 39, 49, 1403, 1501, 1743, 1824, 2089, 2296
Tsp45I GTSAC 8 cut(s) 39, 49, 1403, 1501, 1743, 1824, 2089, 2296
TspDTI ATGAA 9 cut(s) 32, 58, 338, 869, 960, 1035, 1664, 2250, 2295
TspGWI ACGGA 2 cut(s) 1668, 2130
VpaK11BI GGWCC 4 cut(s) 1025, 1095, 1391, 2102
XapI RAATTY 3 cut(s) 278, 1968, 1987
XceI RCATGY 4 cut(s) 193, 670, 2001, 2294
XcmI CCANNNNNNNNNTGG 2 cut(s) 232, 1477
XmiI GTMKAC 4 cut(s) 454, 469, 1425, 1615
XspI CTAG 9 cut(s) 366, 383, 1118, 1220, 1329, 1551, 1619, 1901, 2004
Zsp2I ATGCAT 1 cut(s) 1418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.