Rh4BG022700

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001127
Physical Location & Seq
Reverse (-)
3469108 .. 3473177
4070 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG022700.1

Sequence Viewer

Length: 2343 bp
ATGACCTGCCCAATAAGTTCAGTAGTACTTGGTTTCCTATTATGCCTTCTCTGTGTTCTGAATGACCAGGGAATATCGATAACAGTGGCCGCGGCTAAGACTAATAGCCGTGTTCACATTGTGTATCTGGGAGAGAGGCAGCATGACAATCCCAAGTTGATCACGGATTCTCATCATGATTTGCTTGCCACCATAGTTGGAAGTAAGAGCTTGGCCTCGGAATTGATGGTATACAGTTACAGACATGGTTTCTCTGGATTTGCAGCCAAGCTTACGGAGGCGCAAGCCCAAAAATGTGCAGAGTTGCCTGATGTTGTTCGAGTCATACCAAATACTCTATACAAGCTGCAAACTACTAGGAGTTGGGATTTTCTTGGCCTCTCTCCTCAGTCACCTTCAAGTAATATTCTTCCCAGTAGCGACATGGGTGATGGAGTTATCATAGGTGTCCTTGACTCAGGTATATGGCCAGAGTCTAAATCTTTCAATGAAGAAGGCCTAGGGCCAGTTCCATCCCACTGGAAGGGTATCTGTGAATCCGGTGAAAAATTTAATGCCACATTGCATTGCAATAGAAAAATCATTGGAGCACGTTGGTTCAACGATGGAATACTTGCGGAGTATGGACAGCCGTTGAACACATCTGAAAGAACTGAATTCATGTCCCCAAGAGATGCACATGGACATGGCACTCACACTGCTAGCACTGCAGCTGGTTCCTTTGTGACTAATGTTAGTTACAAGGGCCTTGGTCATGGGACAGTTAGAGGGGGTGCCCCAAATGCTCGATTGGCCATTTACAAGGTTTGCTGGAATGTGCTTGTCAGTCCATGCTCAGGAGCTGATATACTCAAAGCTTTTGATGAAGCCATACATGATGGAGTTGATGTATTGTCACTCTCGATTGGGGGTTCAGTTCCACTCTATTCGGATGTGGATGAACGTGATGTCATTGCTACTGGCTCCTTTCATGCTGTGGCCAGGGGAATTACTGTTGTTTGTGCAGCATCAAATGATGGACCTTCGGCGCAGACAGTTCAGAACACATCACCTTGGATCATAACTGTTGCAGCAAGCACCACGGATCGAGCATTTCCTACTTCCATAACTTTAGGAAATAACAAAACTTTCCTGGGTCAAGCTATGTTTACAGGACTTGAGATTGGTTTTACAAGCTTGATATATCCGGAGTCTGGAGGGCTTGAACCTACTGCTACAGGTGTCTGTGAATCCCTCTCACTTAACAAAACCATGGTATCTGGAAAGGTGGTTCTATGCTTCACTTCACTCGGTTGGAGAGCTGTACCAAGTGCTTCAGCTGCTGTGAAAGAAGCTGGCGGTGTTGGCCTAATCGTAGCAAAGAATCCAACTAATGGCTTGTATCCATGTAGTGATGACTTTCCTTGTATTGAAGTTGACTATGAGATAGGCACCCGAATAGTGTTTTATATCCGATCTACCAGATATCCTCTTGTGAAGTTGAACCGTTCGAAAACAATTGCGGGCAAGCCAATATTAGCCAAGGTTGCCGATTTTTCATCTCGGGGACCAAACTCCGCAGCACCAGCAATTCTCAAGCCAGATATAGCTGCGCCTGGTGTGAACATATTAGCTGCAACTTCTCCACTGCATTCATTTGTGGATGCTGGATATGTCATGCTCTCAGGAACATCGATGGCAACTCCTCATGTCTCAGGCATTGTGGCTCTCCTCAAAGCACTGCATCCTAACTGGTCTCCAGCAGCCATTCGATCAGCACTAGTCACAAGTGCATGGAGGAATGGTCCATCTGGTTTACCAATTTTTGCCGAGGGATCTCCTCAAAAGTTGACCAATCCTTTCGACTTTGGAGGTGGCATTGTGCACCCGAATGCAGCAGCAAACCCGGGTCTTGTATATGACATGGGTGCAGCAGACTACATACATTATCTATGTGCCATGGGCTACAAAAACTCTGCCATATCAAGGCTCACAGGCCAAGCCACAACATGTCCCATAATGAGACCTTCTATTTTGGACATTAACCTACCTTCCATAACCATACCAAGCCTCAGACATCCCATTACTGTAACAAGAACTGTCACAAATGTAGGAGACCCTAAATCCGTTTATGAAGCTACAATTGATCCTCCATTGGGAACAGTAGTATCTGTAAAACCCAATCCTTTGGTATTCAACTCCACGGTTCAGAAACTCACTTTCGAGATTACAATTTCCACAACCCACCAGATGAACACAGGGTACTACTTTGGAAGCCTTACATGGACTGATAGAGTGCATGCTGTGAGAATTCCCTTGTCTGTGAGGACAGAGTTTTTACAACATTTTGCTGATTATCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

780

Amino Acids

83.03

Weight (kDa)

7.08

Isoelectric Point (pI)

30.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 38 - 117 3.3e-17 Peptidase inhibitor I9
Peptidase_S8 PF00082 143 - 596 2.1e-46 Subtilase family
fn3_6 PF17766 673 - 768 4.4e-29 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 14
AccB1I GGYRCC 2 cut(s) 773, 1430
AccB7I CCANNNNNTGG 1 cut(s) 1373
AccI GTMKAC 1 cut(s) 231
AccII CGCG 1 cut(s) 92
AccIII TCCGGA 1 cut(s) 1186
AciI CCGC 6 cut(s) 90, 92, 617, 1338, 1502, 1557
AclWI GGATC 4 cut(s) 1064, 1092, 1822, 2120
AcoI YGGCCR 4 cut(s) 87, 467, 792, 978
AcsI RAATTY 3 cut(s) 548, 656, 2289
AcuI CTGAAG 1 cut(s) 1299
AdeI CACNNNGTG 1 cut(s) 121
AfaI GTAC 3 cut(s) 27, 1305, 2243
AfiI CCNNNNNNNGG 6 cut(s) 523, 836, 1193, 1373, 1965, 2135
AflIII ACRYGT 1 cut(s) 1988
AgsI TTSAA 8 cut(s) 399, 487, 601, 637, 1205, 1412, 1483, 2176
AhlI ACTAGT 1 cut(s) 1759
AjnI CCWGG 4 cut(s) 66, 980, 1131, 1594
AloI GAACNNNNNNTCC 2 cut(s) 1254, 1286
Alw21I GWGCWC 2 cut(s) 592, 1866
Alw26I GTCTC 4 cut(s) 1696, 1740, 1996, 2088
Alw44I GTGCAC 1 cut(s) 1862
AlwI GGATC 4 cut(s) 1064, 1092, 1822, 2120
AlwNI CAGNNNCTG 2 cut(s) 842, 1322
Ama87I CYCGRG 2 cut(s) 1542, 1885
Aor13HI TCCGGA 1 cut(s) 1186
ApaLI GTGCAC 1 cut(s) 1862
ApoI RAATTY 3 cut(s) 548, 656, 2289
AspA2I CCTAGG 1 cut(s) 499
AspLEI GCGC 3 cut(s) 283, 1030, 1594
AspS9I GGNCC 5 cut(s) 503, 745, 1019, 1547, 1784
AsuC2I CCSGG 2 cut(s) 1886, 1887
AsuHPI GGTGA 4 cut(s) 384, 440, 554, 1041
AsuII TTCGAA 1 cut(s) 1490
AsuNHI GCTAGC 1 cut(s) 701
AvaI CYCGRG 2 cut(s) 1542, 1885
AvaII GGWCC 3 cut(s) 1019, 1547, 1784
AvrII CCTAGG 1 cut(s) 499
BaeGI GKGCMC 2 cut(s) 778, 1866
BaeI ACNNNNGTAYC 4 cut(s) 1239, 1272, 2130, 2163
BalI TGGCCA 3 cut(s) 469, 794, 980
BanI GGYRCC 2 cut(s) 773, 1430
Bbv12I GWGCWC 2 cut(s) 592, 1866
BccI CCATC 8 cut(s) 220, 425, 520, 599, 872, 1010, 1669, 1795
BceAI ACGGC 2 cut(s) 93, 616
BciT130I CCWGG 4 cut(s) 68, 982, 1133, 1596
BciVI GTATCC 1 cut(s) 1392
BclI TGATCA 1 cut(s) 159
BcnI CCSGG 2 cut(s) 1886, 1887
BcoDI GTCTC 4 cut(s) 1696, 1740, 1996, 2088
BcuI ACTAGT 1 cut(s) 1759
BfaI CTAG 4 cut(s) 357, 500, 702, 1760
BfmI CTRYAG 2 cut(s) 708, 1215
BfuAI ACCTGC 1 cut(s) 14
BfuI GTATCC 1 cut(s) 1392
BlnI CCTAGG 1 cut(s) 499
BmcAI AGTACT 1 cut(s) 27
Bme1390I CCNGG 6 cut(s) 68, 982, 1133, 1596, 1886, 1887
Bme18I GGWCC 3 cut(s) 1019, 1547, 1784
BmeT110I CYCGRG 2 cut(s) 1542, 1885
BmgT120I GGNCC 5 cut(s) 503, 745, 1019, 1547, 1784
BmiI GGNNCC 5 cut(s) 718, 775, 964, 1432, 1548
BmrFI CCNGG 6 cut(s) 68, 982, 1133, 1596, 1886, 1887
BmrI ACTGGG 1 cut(s) 408
BmsI GCATC 4 cut(s) 664, 1016, 1633, 1732
BmtI GCTAGC 1 cut(s) 705
BmuI ACTGGG 1 cut(s) 408
BpmI CTGGAG 2 cut(s) 1215, 1722
Bpu10I CCTNAGC 1 cut(s) 835
Bpu14I TTCGAA 1 cut(s) 1490
BpuEI CTTGAG 2 cut(s) 1178, 1559
BpuMI CCSGG 2 cut(s) 1886, 1887
Bsa29I ATCGAT 2 cut(s) 77, 1673
BsaBI GATNNNNATC 1 cut(s) 171
BsaI GGTCTC 3 cut(s) 1740, 1996, 2088
BsaWI WCCGGW 2 cut(s) 539, 1186
BsaXI ACNNNNNCTCC 4 cut(s) 873, 903, 1768, 1798
Bsc4I CCNNNNNNNGG 6 cut(s) 523, 836, 1193, 1373, 1965, 2135
Bse1I ACTGG 5 cut(s) 414, 506, 524, 964, 1736
Bse3DI GCAATG 3 cut(s) 560, 565, 951
Bse8I GATNNNNATC 1 cut(s) 171
BseAI TCCGGA 1 cut(s) 1186
BseBI CCWGG 4 cut(s) 68, 982, 1133, 1596
BseCI ATCGAT 2 cut(s) 77, 1673
BseGI GGATG 6 cut(s) 512, 937, 943, 1648, 1723, 2056
BseJI GATNNNNATC 1 cut(s) 171
BseLI CCNNNNNNNGG 6 cut(s) 523, 836, 1193, 1373, 1965, 2135
BseMI GCAATG 3 cut(s) 560, 565, 951
BseMII CTCAG 6 cut(s) 401, 471, 849, 1677, 1707, 2065
BseNI ACTGG 5 cut(s) 414, 506, 524, 964, 1736
BseRI GAGGAG 4 cut(s) 375, 1674, 1700, 1809
BseSI GKGCMC 2 cut(s) 778, 1866
BsgI GTGCAG 3 cut(s) 318, 1023, 1929
Bsh1236I CGCG 1 cut(s) 92
BshNI GGYRCC 2 cut(s) 773, 1430
BshVI ATCGAT 2 cut(s) 77, 1673
BsiHKAI GWGCWC 2 cut(s) 592, 1866
BsiHKCI CYCGRG 2 cut(s) 1542, 1885
BsiSI CCGG 3 cut(s) 540, 1187, 1886
BslFI GGGAC 4 cut(s) 649, 772, 1560, 1977
BslI CCNNNNNNNGG 6 cut(s) 523, 836, 1193, 1373, 1965, 2135
BsmAI GTCTC 4 cut(s) 1696, 1740, 1996, 2088
BsmFI GGGAC 4 cut(s) 649, 772, 1560, 1977
BsmI GAATGC 2 cut(s) 1630, 1876
Bso31I GGTCTC 3 cut(s) 1740, 1996, 2088
BsoBI CYCGRG 2 cut(s) 1542, 1885
Bsp119I TTCGAA 1 cut(s) 1490
Bsp1286I GDGCHC 3 cut(s) 592, 778, 1866
Bsp13I TCCGGA 1 cut(s) 1186
Bsp143I GATC 7 cut(s) 159, 1056, 1084, 1454, 1751, 1814, 2125
Bsp19I CCATGG 2 cut(s) 1251, 1938
BspACI CCGC 6 cut(s) 90, 92, 617, 1338, 1502, 1557
BspCNI CTCAG 6 cut(s) 400, 470, 848, 1676, 1706, 2064
BspDI ATCGAT 2 cut(s) 77, 1673
BspEI TCCGGA 1 cut(s) 1186
BspFNI CGCG 1 cut(s) 92
BspHI TCATGA 1 cut(s) 175
BspLI GGNNCC 5 cut(s) 718, 775, 964, 1432, 1548
BspMAI CTGCAG 1 cut(s) 712
BspMI ACCTGC 1 cut(s) 14
BspOI GCTAGC 1 cut(s) 705
BspPI GGATC 4 cut(s) 1064, 1092, 1822, 2120
BspT104I TTCGAA 1 cut(s) 1490
BspT107I GGYRCC 2 cut(s) 773, 1430
BspTNI GGTCTC 3 cut(s) 1740, 1996, 2088
BsrDI GCAATG 3 cut(s) 560, 565, 951
BsrI ACTGG 5 cut(s) 414, 506, 524, 964, 1736
BssMI GATC 7 cut(s) 159, 1056, 1084, 1454, 1751, 1814, 2125
BssNAI GTATAC 1 cut(s) 232
BssT1I CCWWGG 6 cut(s) 499, 748, 1052, 1251, 1521, 1938
Bst1107I GTATAC 1 cut(s) 232
Bst2UI CCWGG 4 cut(s) 68, 982, 1133, 1596
BstBI TTCGAA 1 cut(s) 1490
BstC8I GCNNGC 8 cut(s) 186, 285, 703, 1075, 1336, 1504, 1508, 2280
BstDEI CTNAG 7 cut(s) 96, 387, 457, 835, 1663, 1693, 2051
BstDSI CCRYGG 5 cut(s) 90, 1080, 1251, 1938, 2181
BstF5I GGATG 6 cut(s) 512, 937, 943, 1648, 1723, 2056
BstFNI CGCG 1 cut(s) 92
BstHHI GCGC 3 cut(s) 283, 1030, 1594
BstKTI GATC 7 cut(s) 162, 1059, 1087, 1457, 1754, 1817, 2128
BstMAI GTCTC 4 cut(s) 1696, 1740, 1996, 2088
BstMBI GATC 7 cut(s) 159, 1056, 1084, 1454, 1751, 1814, 2125
BstMWI GCNNNNNNNGC 7 cut(s) 707, 782, 791, 1319, 1344, 1526, 1565
BstNI CCWGG 4 cut(s) 68, 982, 1133, 1596
BstNSI RCATGY 2 cut(s) 1992, 2282
BstSCI CCNGG 6 cut(s) 66, 980, 1131, 1594, 1884, 1885
BstSFI CTRYAG 2 cut(s) 708, 1215
BstSLI GKGCMC 2 cut(s) 778, 1866
BstUI CGCG 1 cut(s) 92
BstX2I RGATCY 1 cut(s) 1814
BstXI CCANNNNNNTGG 2 cut(s) 519, 2167
BstYI RGATCY 1 cut(s) 1814
BstZ17I GTATAC 1 cut(s) 232
Bsu15I ATCGAT 2 cut(s) 77, 1673
BsuI GTATCC 1 cut(s) 1392
BsuTUI ATCGAT 2 cut(s) 77, 1673
BtgI CCRYGG 5 cut(s) 90, 1080, 1251, 1938, 2181
BtsCI GGATG 6 cut(s) 512, 937, 943, 1648, 1723, 2056
BtsI GCAGTG 4 cut(s) 696, 705, 1625, 1718
BtsIMutI CAGTG 6 cut(s) 90, 517, 696, 705, 1625, 1718
BveI ACCTGC 1 cut(s) 14
Cac8I GCNNGC 8 cut(s) 186, 285, 703, 1075, 1336, 1504, 1508, 2280
CaiI CAGNNNCTG 2 cut(s) 842, 1322
CciI TCATGA 1 cut(s) 175
CfoI GCGC 3 cut(s) 283, 1030, 1594
Cfr13I GGNCC 5 cut(s) 503, 745, 1019, 1547, 1784
Cfr42I CCGCGG 1 cut(s) 93
Cfr9I CCCGGG 1 cut(s) 1885
ClaI ATCGAT 2 cut(s) 77, 1673
Csp6I GTAC 3 cut(s) 26, 1304, 2242
CspCI CAANNNNNGTGG 2 cut(s) 178, 213
CviQI GTAC 3 cut(s) 26, 1304, 2242
DdeI CTNAG 7 cut(s) 96, 387, 457, 835, 1663, 1693, 2051
DpnI GATC 7 cut(s) 161, 1058, 1086, 1456, 1753, 1816, 2127
DpnII GATC 7 cut(s) 159, 1056, 1084, 1454, 1751, 1814, 2125
DraIII CACNNNGTG 1 cut(s) 121
EaeI YGGCCR 4 cut(s) 87, 467, 792, 978
Eco130I CCWWGG 6 cut(s) 499, 748, 1052, 1251, 1521, 1938
Eco147I AGGCCT 1 cut(s) 498
Eco31I GGTCTC 3 cut(s) 1740, 1996, 2088
Eco32I GATATC 1 cut(s) 1466
Eco47I GGWCC 3 cut(s) 1019, 1547, 1784
Eco57I CTGAAG 1 cut(s) 1299
Eco88I CYCGRG 2 cut(s) 1542, 1885
EcoO109I RGGNCCY 1 cut(s) 745
EcoRI GAATTC 2 cut(s) 656, 2289
EcoRII CCWGG 4 cut(s) 66, 980, 1131, 1594
EcoRV GATATC 1 cut(s) 1466
EcoT14I CCWWGG 6 cut(s) 499, 748, 1052, 1251, 1521, 1938
ErhI CCWWGG 6 cut(s) 499, 748, 1052, 1251, 1521, 1938
FaqI GGGAC 4 cut(s) 649, 772, 1560, 1977
FauI CCCGC 1 cut(s) 1495
FbaI TGATCA 1 cut(s) 159
FblI GTMKAC 1 cut(s) 231
FokI GGATG 6 cut(s) 499, 944, 950, 1655, 1710, 2043
FspBI CTAG 4 cut(s) 357, 500, 702, 1760
GlaI GCGC 3 cut(s) 282, 1029, 1593
GsuI CTGGAG 2 cut(s) 1215, 1722
HapII CCGG 3 cut(s) 540, 1187, 1886
HhaI GCGC 3 cut(s) 283, 1030, 1594
Hin6I GCGC 3 cut(s) 281, 1028, 1592
HinP1I GCGC 3 cut(s) 281, 1028, 1592
HincII GTYRAC 2 cut(s) 1417, 1830
HindII GTYRAC 2 cut(s) 1417, 1830
HindIII AAGCTT 3 cut(s) 269, 855, 1174
HinfI GANTC 8 cut(s) 167, 321, 455, 473, 536, 1190, 1229, 1363
HpaII CCGG 3 cut(s) 540, 1187, 1886
HphI GGTGA 4 cut(s) 384, 440, 554, 1041
Hpy166II GTNNAC 8 cut(s) 115, 232, 1149, 1417, 1603, 1796, 1830, 1864
Hpy188I TCNGA 8 cut(s) 60, 220, 646, 931, 1041, 1454, 2054, 2190
Hpy188III TCNNGA 9 cut(s) 176, 255, 837, 901, 1187, 1194, 1260, 1665, 2203
Hpy8I GTNNAC 8 cut(s) 115, 232, 1149, 1417, 1603, 1796, 1830, 1864
HpyAV CCTTC 7 cut(s) 56, 405, 488, 517, 1032, 2016, 2040
HpyCH4IV ACGT 2 cut(s) 592, 943
HpyF10VI GCNNNNNNNGC 7 cut(s) 707, 782, 791, 1319, 1344, 1526, 1565
HpyF3I CTNAG 7 cut(s) 96, 387, 457, 835, 1663, 1693, 2051
HpySE526I ACGT 2 cut(s) 592, 943
HspAI GCGC 3 cut(s) 281, 1028, 1592
Kpn2I TCCGGA 1 cut(s) 1186
Ksp22I TGATCA 1 cut(s) 159
KspI CCGCGG 1 cut(s) 93
Kzo9I GATC 7 cut(s) 159, 1056, 1084, 1454, 1751, 1814, 2125
LmnI GCTCC 3 cut(s) 587, 839, 968
LweI GCATC 4 cut(s) 664, 1016, 1633, 1732
MaeI CTAG 4 cut(s) 357, 500, 702, 1760
MaeII ACGT 2 cut(s) 592, 943
MaeIII GTNAC 8 cut(s) 236, 390, 724, 737, 894, 1762, 2068, 2080
MalI GATC 7 cut(s) 161, 1058, 1086, 1456, 1753, 1816, 2127
MboI GATC 7 cut(s) 159, 1056, 1084, 1454, 1751, 1814, 2125
MboII GAAGA 2 cut(s) 401, 503
MfeI CAATTG 2 cut(s) 1497, 2121
MflI RGATCY 1 cut(s) 1814
MhlI GDGCHC 3 cut(s) 592, 778, 1866
MlsI TGGCCA 3 cut(s) 469, 794, 980
MluNI TGGCCA 3 cut(s) 469, 794, 980
MlyI GAGTC 4 cut(s) 330, 449, 482, 1199
MmeI TCCRAC 3 cut(s) 178, 1274, 1391
Mox20I TGGCCA 3 cut(s) 469, 794, 980
MroI TCCGGA 1 cut(s) 1186
MscI TGGCCA 3 cut(s) 469, 794, 980
MseI TTAA 3 cut(s) 552, 1242, 2022
MslI CAYNNNNRTG 2 cut(s) 684, 1869
Msp20I TGGCCA 3 cut(s) 469, 794, 980
MspA1I CMGCKG 3 cut(s) 92, 713, 1319
MspI CCGG 3 cut(s) 540, 1187, 1886
MspR9I CCNGG 6 cut(s) 68, 982, 1133, 1596, 1886, 1887
MunI CAATTG 2 cut(s) 1497, 2121
Mva1269I GAATGC 2 cut(s) 1630, 1876
MvaI CCWGG 4 cut(s) 68, 982, 1133, 1596
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 7 cut(s) 707, 782, 791, 1319, 1344, 1526, 1565
NciI CCSGG 2 cut(s) 1886, 1887
NcoI CCATGG 2 cut(s) 1251, 1938
NdeII GATC 7 cut(s) 159, 1056, 1084, 1454, 1751, 1814, 2125
NheI GCTAGC 1 cut(s) 701
NlaIV GGNNCC 5 cut(s) 718, 775, 964, 1432, 1548
NmeAIII GCCGAG 1 cut(s) 1834
NmuCI GTSAC 5 cut(s) 390, 724, 894, 1762, 2080
NspI RCATGY 2 cut(s) 1992, 2282
NspV TTCGAA 1 cut(s) 1490
PaeI GCATGC 1 cut(s) 2282
PagI TCATGA 1 cut(s) 175
PceI AGGCCT 1 cut(s) 498
PciI ACATGT 1 cut(s) 1988
PctI GAATGC 2 cut(s) 1630, 1876
PfeI GAWTC 4 cut(s) 167, 536, 1229, 1363
PflMI CCANNNNNTGG 1 cut(s) 1373
PleI GAGTC 4 cut(s) 329, 449, 481, 1198
PpsI GAGTC 4 cut(s) 329, 449, 481, 1198
PscI ACATGT 1 cut(s) 1988
Psp6I CCWGG 4 cut(s) 66, 980, 1131, 1594
PspGI CCWGG 4 cut(s) 66, 980, 1131, 1594
PspN4I GGNNCC 5 cut(s) 718, 775, 964, 1432, 1548
PspPI GGNCC 5 cut(s) 503, 745, 1019, 1547, 1784
PsrI GAACNNNNNNTAC 2 cut(s) 2225, 2257
PstI CTGCAG 1 cut(s) 712
PstNI CAGNNNCTG 2 cut(s) 842, 1322
PsuI RGATCY 1 cut(s) 1814
PvuII CAGCTG 2 cut(s) 713, 1319
RsaI GTAC 3 cut(s) 27, 1305, 2243
RsaNI GTAC 3 cut(s) 26, 1304, 2242
RseI CAYNNNNRTG 2 cut(s) 684, 1869
SacII CCGCGG 1 cut(s) 93
SaqAI TTAA 3 cut(s) 552, 1242, 2022
Sau3AI GATC 7 cut(s) 159, 1056, 1084, 1454, 1751, 1814, 2125
Sau96I GGNCC 5 cut(s) 503, 745, 1019, 1547, 1784
ScaI AGTACT 1 cut(s) 27
SchI GAGTC 4 cut(s) 330, 449, 482, 1199
ScrFI CCNGG 6 cut(s) 68, 982, 1133, 1596, 1886, 1887
SduI GDGCHC 3 cut(s) 592, 778, 1866
SfaNI GCATC 4 cut(s) 664, 1016, 1633, 1732
SfcI CTRYAG 2 cut(s) 708, 1215
Sfr303I CCGCGG 1 cut(s) 93
SfuI TTCGAA 1 cut(s) 1490
SgrBI CCGCGG 1 cut(s) 93
SinI GGWCC 3 cut(s) 1019, 1547, 1784
SmaI CCCGGG 1 cut(s) 1887
SmiMI CAYNNNNRTG 2 cut(s) 684, 1869
SmlI CTYRAG 2 cut(s) 1157, 1574
SmoI CTYRAG 2 cut(s) 1157, 1574
SpeI ACTAGT 1 cut(s) 1759
SphI GCATGC 1 cut(s) 2282
SseBI AGGCCT 1 cut(s) 498
SsiI CCGC 6 cut(s) 90, 92, 617, 1338, 1502, 1557
SspI AATATT 2 cut(s) 406, 1515
SspMI CTAG 4 cut(s) 357, 500, 702, 1760
StuI AGGCCT 1 cut(s) 498
StyD4I CCNGG 6 cut(s) 66, 980, 1131, 1594, 1884, 1885
StyI CCWWGG 6 cut(s) 499, 748, 1052, 1251, 1521, 1938
TaiI ACGT 2 cut(s) 595, 946
TatI WGTACW 1 cut(s) 25
TauI GCSGC 2 cut(s) 92, 95
TfiI GAWTC 4 cut(s) 167, 536, 1229, 1363
Tru1I TTAA 3 cut(s) 552, 1242, 2022
Tru9I TTAA 3 cut(s) 552, 1242, 2022
TscAI CASTG 6 cut(s) 90, 524, 703, 712, 1632, 1725
TseFI GTSAC 5 cut(s) 390, 724, 894, 1762, 2080
Tsp45I GTSAC 5 cut(s) 390, 724, 894, 1762, 2080
TspDTI ATGAA 9 cut(s) 504, 649, 879, 954, 959, 1527, 1623, 2127, 2246
TspGWI ACGGA 4 cut(s) 179, 290, 1097, 2095
TspMI CCCGGG 1 cut(s) 1885
TspRI CASTG 6 cut(s) 90, 524, 703, 712, 1632, 1725
Van91I CCANNNNNTGG 1 cut(s) 1373
VneI GTGCAC 1 cut(s) 1862
VpaK11BI GGWCC 3 cut(s) 1019, 1547, 1784
XapI RAATTY 3 cut(s) 548, 656, 2289
XceI RCATGY 2 cut(s) 1992, 2282
XcmI CCANNNNNNNNNTGG 1 cut(s) 421
XmaI CCCGGG 1 cut(s) 1885
XmaJI CCTAGG 1 cut(s) 499
XmiI GTMKAC 1 cut(s) 231
XspI CTAG 4 cut(s) 357, 500, 702, 1760
ZrmI AGTACT 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.