subtilisin-like protease
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 1054, 2040 |
| AccB1I | GGYRCC | 2 cut(s) | 1244, 1913 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 107 |
| AccII | CGCG | 1 cut(s) | 1610 |
| AciI | CCGC | 6 cut(s) | 222, 356, 720, 1362, 1608, 1641 |
| AclWI | GGATC | 8 cut(s) | 152, 165, 295, 929, 1088, 1107, 1784, 1835 |
| AcoI | YGGCCR | 4 cut(s) | 473, 738, 804, 1002 |
| AcsI | RAATTY | 1 cut(s) | 653 |
| AcuI | CTGAAG | 2 cut(s) | 1836, 1850 |
| AdeI | CACNNNGTG | 1 cut(s) | 2236 |
| AfaI | GTAC | 2 cut(s) | 692, 812 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 107, 471, 1427, 1879, 2141 |
| AhlI | ACTAGT | 1 cut(s) | 1765 |
| AjiI | CACGTC | 1 cut(s) | 1992 |
| AjnI | CCWGG | 4 cut(s) | 1076, 1612, 1891, 2140 |
| AleI | CACNNNNGTG | 2 cut(s) | 1280, 1938 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2021, 2053 |
| Alw21I | GWGCWC | 2 cut(s) | 317, 790 |
| Alw26I | GTCTC | 4 cut(s) | 416, 820, 1901, 2162 |
| Alw44I | GTGCAC | 1 cut(s) | 786 |
| AlwI | GGATC | 8 cut(s) | 152, 165, 295, 929, 1088, 1107, 1784, 1835 |
| ApaLI | GTGCAC | 1 cut(s) | 786 |
| ApoI | RAATTY | 1 cut(s) | 653 |
| AspA2I | CCTAGG | 2 cut(s) | 382, 766 |
| AspLEI | GCGC | 1 cut(s) | 1612 |
| AspS9I | GGNCC | 7 cut(s) | 509, 836, 1043, 1356, 1565, 1740, 2099 |
| AsuC2I | CCSGG | 1 cut(s) | 14 |
| AsuHPI | GGTGA | 3 cut(s) | 28, 1880, 2035 |
| AvaII | GGWCC | 4 cut(s) | 1043, 1565, 1740, 2099 |
| AvrII | CCTAGG | 2 cut(s) | 382, 766 |
| BaeGI | GKGCMC | 2 cut(s) | 790, 1916 |
| BalI | TGGCCA | 3 cut(s) | 475, 740, 1004 |
| BamHI | GGATCC | 1 cut(s) | 157 |
| BanI | GGYRCC | 2 cut(s) | 1244, 1913 |
| BanII | GRGCYC | 2 cut(s) | 799, 1505 |
| Bbv12I | GWGCWC | 2 cut(s) | 317, 790 |
| BceAI | ACGGC | 2 cut(s) | 271, 791 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 548, 582 |
| BciT130I | CCWGG | 4 cut(s) | 1078, 1614, 1893, 2142 |
| BcnI | CCSGG | 1 cut(s) | 14 |
| BcoDI | GTCTC | 4 cut(s) | 416, 820, 1901, 2162 |
| BcuI | ACTAGT | 1 cut(s) | 1765 |
| BfaI | CTAG | 6 cut(s) | 366, 383, 767, 1392, 1560, 1766 |
| BfmI | CTRYAG | 2 cut(s) | 241, 1029 |
| BfuAI | ACCTGC | 2 cut(s) | 1054, 2040 |
| BglII | AGATCT | 1 cut(s) | 1481 |
| BlnI | CCTAGG | 2 cut(s) | 382, 766 |
| Bme1390I | CCNGG | 5 cut(s) | 14, 1078, 1614, 1893, 2142 |
| Bme18I | GGWCC | 4 cut(s) | 1043, 1565, 1740, 2099 |
| BmgBI | CACGTC | 1 cut(s) | 1992 |
| BmgT120I | GGNCC | 7 cut(s) | 509, 836, 1043, 1356, 1565, 1740, 2099 |
| BmiI | GGNNCC | 6 cut(s) | 159, 730, 1246, 1567, 1742, 1915 |
| BmrFI | CCNGG | 5 cut(s) | 14, 1078, 1614, 1893, 2142 |
| BmrI | ACTGGG | 2 cut(s) | 840, 1166 |
| BmsI | GCATC | 6 cut(s) | 206, 462, 676, 847, 1216, 2154 |
| BmuI | ACTGGG | 2 cut(s) | 840, 1166 |
| BpmI | CTGGAG | 1 cut(s) | 339 |
| Bpu10I | CCTNAGC | 1 cut(s) | 162 |
| BpuMI | CCSGG | 1 cut(s) | 14 |
| Bsa29I | ATCGAT | 1 cut(s) | 1788 |
| BsaBI | GATNNNNATC | 1 cut(s) | 180 |
| BsaI | GGTCTC | 2 cut(s) | 416, 1901 |
| BsaJI | CCNNGG | 7 cut(s) | 382, 766, 778, 791, 1076, 1104, 1891 |
| BsaWI | WCCGGW | 1 cut(s) | 464 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 426, 456, 817, 847, 1468, 1498, 1851, 1881 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 107, 471, 1427, 1879, 2141 |
| Bse118I | RCCGGY | 1 cut(s) | 1358 |
| Bse1I | ACTGG | 3 cut(s) | 101, 846, 1161 |
| Bse3DI | GCAATG | 5 cut(s) | 1042, 1575, 1695, 1724, 1758 |
| Bse8I | GATNNNNATC | 1 cut(s) | 180 |
| BseBI | CCWGG | 4 cut(s) | 1078, 1614, 1893, 2142 |
| BseCI | ATCGAT | 1 cut(s) | 1788 |
| BseDI | CCNNGG | 7 cut(s) | 382, 766, 778, 791, 1076, 1104, 1891 |
| BseGI | GGATG | 3 cut(s) | 1291, 1654, 2327 |
| BseJI | GATNNNNATC | 1 cut(s) | 180 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 107, 471, 1427, 1879, 2141 |
| BseMI | GCAATG | 5 cut(s) | 1042, 1575, 1695, 1724, 1758 |
| BseMII | CTCAG | 4 cut(s) | 153, 1067, 1443, 1490 |
| BseNI | ACTGG | 3 cut(s) | 101, 846, 1161 |
| BseRI | GAGGAG | 2 cut(s) | 1041, 1872 |
| BseSI | GKGCMC | 2 cut(s) | 790, 1916 |
| Bsh1236I | CGCG | 1 cut(s) | 1610 |
| BshNI | GGYRCC | 2 cut(s) | 1244, 1913 |
| BshVI | ATCGAT | 1 cut(s) | 1788 |
| BsiHKAI | GWGCWC | 2 cut(s) | 317, 790 |
| BsiSI | CCGG | 3 cut(s) | 13, 465, 1359 |
| BsiWI | CGTACG | 1 cut(s) | 690 |
| BslFI | GGGAC | 2 cut(s) | 1687, 1726 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 107, 471, 1427, 1879, 2141 |
| BsmAI | GTCTC | 4 cut(s) | 416, 820, 1901, 2162 |
| BsmFI | GGGAC | 2 cut(s) | 1687, 1726 |
| Bso31I | GGTCTC | 2 cut(s) | 416, 1901 |
| Bsp1286I | GDGCHC | 5 cut(s) | 317, 790, 799, 1505, 1916 |
| Bsp1407I | TGTACA | 1 cut(s) | 810 |
| Bsp19I | CCATGG | 1 cut(s) | 1104 |
| BspACI | CCGC | 6 cut(s) | 222, 356, 720, 1362, 1608, 1641 |
| BspCNI | CTCAG | 4 cut(s) | 154, 1066, 1444, 1491 |
| BspDI | ATCGAT | 1 cut(s) | 1788 |
| BspFNI | CGCG | 1 cut(s) | 1610 |
| BspHI | TCATGA | 2 cut(s) | 184, 628 |
| BspLI | GGNNCC | 6 cut(s) | 159, 730, 1246, 1567, 1742, 1915 |
| BspMAI | CTGCAG | 1 cut(s) | 1033 |
| BspMI | ACCTGC | 2 cut(s) | 1054, 2040 |
| BspPI | GGATC | 8 cut(s) | 152, 165, 295, 929, 1088, 1107, 1784, 1835 |
| BspT107I | GGYRCC | 2 cut(s) | 1244, 1913 |
| BspTNI | GGTCTC | 2 cut(s) | 416, 1901 |
| BsrDI | GCAATG | 5 cut(s) | 1042, 1575, 1695, 1724, 1758 |
| BsrFI | RCCGGY | 1 cut(s) | 1358 |
| BsrGI | TGTACA | 1 cut(s) | 810 |
| BsrI | ACTGG | 3 cut(s) | 101, 846, 1161 |
| BssAI | RCCGGY | 1 cut(s) | 1358 |
| BssECI | CCNNGG | 7 cut(s) | 382, 766, 778, 791, 1076, 1104, 1891 |
| BssT1I | CCWWGG | 3 cut(s) | 382, 766, 1104 |
| Bst2UI | CCWGG | 4 cut(s) | 1078, 1614, 1893, 2142 |
| Bst4CI | ACNGT | 8 cut(s) | 1018, 1060, 1090, 1409, 1966, 2086, 2191, 2212 |
| Bst6I | CTCTTC | 3 cut(s) | 397, 948, 1561 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1037, 1244 |
| BstAUI | TGTACA | 1 cut(s) | 810 |
| BstDEI | CTNAG | 7 cut(s) | 162, 1053, 1219, 1452, 1499, 1509, 2057 |
| BstDSI | CCRYGG | 2 cut(s) | 791, 1104 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2139 |
| BstF5I | GGATG | 3 cut(s) | 1291, 1654, 2327 |
| BstFNI | CGCG | 1 cut(s) | 1610 |
| BstHHI | GCGC | 1 cut(s) | 1612 |
| BstMAI | GTCTC | 4 cut(s) | 416, 820, 1901, 2162 |
| BstNI | CCWGG | 4 cut(s) | 1078, 1614, 1893, 2142 |
| BstNSI | RCATGY | 4 cut(s) | 822, 1344, 1988, 2288 |
| BstSCI | CCNGG | 5 cut(s) | 12, 1076, 1612, 1891, 2140 |
| BstSFI | CTRYAG | 2 cut(s) | 241, 1029 |
| BstSLI | GKGCMC | 2 cut(s) | 790, 1916 |
| BstUI | CGCG | 1 cut(s) | 1610 |
| BstX2I | RGATCY | 3 cut(s) | 157, 1112, 1481 |
| BstYI | RGATCY | 3 cut(s) | 157, 1112, 1481 |
| Bsu15I | ATCGAT | 1 cut(s) | 1788 |
| BsuTUI | ATCGAT | 1 cut(s) | 1788 |
| BtgI | CCRYGG | 2 cut(s) | 791, 1104 |
| BtrI | CACGTC | 1 cut(s) | 1992 |
| BtsCI | GGATG | 3 cut(s) | 1291, 1654, 2327 |
| BtsI | GCAGTG | 2 cut(s) | 708, 934 |
| BtsIMutI | CAGTG | 7 cut(s) | 708, 852, 934, 1065, 1414, 1449, 1514 |
| BveI | ACCTGC | 2 cut(s) | 1054, 2040 |
| CciI | TCATGA | 2 cut(s) | 184, 628 |
| CfoI | GCGC | 1 cut(s) | 1612 |
| Cfr10I | RCCGGY | 1 cut(s) | 1358 |
| Cfr13I | GGNCC | 7 cut(s) | 509, 836, 1043, 1356, 1565, 1740, 2099 |
| ClaI | ATCGAT | 1 cut(s) | 1788 |
| Csp6I | GTAC | 2 cut(s) | 691, 811 |
| CviQI | GTAC | 2 cut(s) | 691, 811 |
| DdeI | CTNAG | 7 cut(s) | 162, 1053, 1219, 1452, 1499, 1509, 2057 |
| DraIII | CACNNNGTG | 1 cut(s) | 2236 |
| EaeI | YGGCCR | 4 cut(s) | 473, 738, 804, 1002 |
| Eam1104I | CTCTTC | 3 cut(s) | 397, 948, 1561 |
| EarI | CTCTTC | 3 cut(s) | 397, 948, 1561 |
| EciI | GGCGGA | 1 cut(s) | 1377 |
| Eco130I | CCWWGG | 3 cut(s) | 382, 766, 1104 |
| Eco147I | AGGCCT | 2 cut(s) | 38, 387 |
| Eco24I | GRGCYC | 2 cut(s) | 799, 1505 |
| Eco31I | GGTCTC | 2 cut(s) | 416, 1901 |
| Eco47I | GGWCC | 4 cut(s) | 1043, 1565, 1740, 2099 |
| Eco57I | CTGAAG | 2 cut(s) | 1836, 1850 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2139 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1565 |
| EcoRII | CCWGG | 4 cut(s) | 1076, 1612, 1891, 2140 |
| EcoT14I | CCWWGG | 3 cut(s) | 382, 766, 1104 |
| EcoT22I | ATGCAT | 1 cut(s) | 420 |
| EcoT38I | GRGCYC | 2 cut(s) | 799, 1505 |
| ErhI | CCWWGG | 3 cut(s) | 382, 766, 1104 |
| FaqI | GGGAC | 2 cut(s) | 1687, 1726 |
| FauI | CCCGC | 1 cut(s) | 1648 |
| FokI | GGATG | 3 cut(s) | 1298, 1661, 2314 |
| FriOI | GRGCYC | 2 cut(s) | 799, 1505 |
| FspBI | CTAG | 6 cut(s) | 366, 383, 767, 1392, 1560, 1766 |
| GlaI | GCGC | 1 cut(s) | 1611 |
| GsuI | CTGGAG | 1 cut(s) | 339 |
| HapII | CCGG | 3 cut(s) | 13, 465, 1359 |
| HhaI | GCGC | 1 cut(s) | 1612 |
| Hin6I | GCGC | 1 cut(s) | 1610 |
| HinP1I | GCGC | 1 cut(s) | 1610 |
| HincII | GTYRAC | 2 cut(s) | 1267, 1435 |
| HindII | GTYRAC | 2 cut(s) | 1267, 1435 |
| HindIII | AAGCTT | 4 cut(s) | 278, 873, 1216, 1832 |
| HinfI | GANTC | 6 cut(s) | 230, 479, 621, 1263, 1365, 1387 |
| HpaII | CCGG | 3 cut(s) | 13, 465, 1359 |
| HphI | GGTGA | 3 cut(s) | 28, 1880, 2035 |
| Hpy166II | GTNNAC | 8 cut(s) | 119, 667, 788, 811, 1267, 1435, 1870, 2239 |
| Hpy188I | TCNGA | 7 cut(s) | 93, 310, 553, 779, 1056, 1139, 2258 |
| Hpy188III | TCNNGA | 7 cut(s) | 161, 185, 318, 629, 1211, 1560, 2021 |
| Hpy8I | GTNNAC | 8 cut(s) | 119, 667, 788, 811, 1267, 1435, 1870, 2239 |
| HpyCH4III | ACNGT | 8 cut(s) | 1018, 1060, 1090, 1409, 1966, 2086, 2191, 2212 |
| HpyCH4IV | ACGT | 3 cut(s) | 547, 1068, 1991 |
| HpyF3I | CTNAG | 7 cut(s) | 162, 1053, 1219, 1452, 1499, 1509, 2057 |
| HpySE526I | ACGT | 3 cut(s) | 547, 1068, 1991 |
| HspAI | GCGC | 1 cut(s) | 1610 |
| KroI | GCCGGC | 1 cut(s) | 1358 |
| KroNI | GCCGGC | 1 cut(s) | 1360 |
| LmnI | GCTCC | 5 cut(s) | 599, 1054, 1552, 1585, 1693 |
| LweI | GCATC | 6 cut(s) | 206, 462, 676, 847, 1216, 2154 |
| MaeI | CTAG | 6 cut(s) | 366, 383, 767, 1392, 1560, 1766 |
| MaeII | ACGT | 3 cut(s) | 547, 1068, 1991 |
| MaeIII | GTNAC | 6 cut(s) | 749, 929, 1768, 1966, 2086, 2290 |
| MboII | GAAGA | 5 cut(s) | 313, 384, 965, 1548, 1795 |
| MfeI | CAATTG | 1 cut(s) | 2344 |
| MflI | RGATCY | 3 cut(s) | 157, 1112, 1481 |
| MhlI | GDGCHC | 5 cut(s) | 317, 790, 799, 1505, 1916 |
| MlsI | TGGCCA | 3 cut(s) | 475, 740, 1004 |
| MluCI | AATT | 8 cut(s) | 409, 653, 940, 1402, 1587, 1661, 2016, 2344 |
| MluNI | TGGCCA | 3 cut(s) | 475, 740, 1004 |
| MlyI | GAGTC | 3 cut(s) | 488, 1272, 1374 |
| MmeI | TCCRAC | 3 cut(s) | 506, 803, 1607 |
| Mox20I | TGGCCA | 3 cut(s) | 475, 740, 1004 |
| Mph1103I | ATGCAT | 1 cut(s) | 420 |
| MroNI | GCCGGC | 1 cut(s) | 1358 |
| MscI | TGGCCA | 3 cut(s) | 475, 740, 1004 |
| MseI | TTAA | 6 cut(s) | 170, 413, 870, 945, 1401, 2193 |
| MslI | CAYNNNNRTG | 3 cut(s) | 897, 1280, 1938 |
| Msp20I | TGGCCA | 3 cut(s) | 475, 740, 1004 |
| MspA1I | CMGCKG | 1 cut(s) | 722 |
| MspI | CCGG | 3 cut(s) | 13, 465, 1359 |
| MspR9I | CCNGG | 5 cut(s) | 14, 1078, 1614, 1893, 2142 |
| MunI | CAATTG | 1 cut(s) | 2344 |
| MvaI | CCWGG | 4 cut(s) | 1078, 1614, 1893, 2142 |
| MvnI | CGCG | 1 cut(s) | 1610 |
| NaeI | GCCGGC | 1 cut(s) | 1360 |
| NciI | CCSGG | 1 cut(s) | 14 |
| NcoI | CCATGG | 1 cut(s) | 1104 |
| NgoMIV | GCCGGC | 1 cut(s) | 1358 |
| NlaIV | GGNNCC | 6 cut(s) | 159, 730, 1246, 1567, 1742, 1915 |
| NmuCI | GTSAC | 3 cut(s) | 929, 1768, 2290 |
| NsiI | ATGCAT | 1 cut(s) | 420 |
| NspI | RCATGY | 4 cut(s) | 822, 1344, 1988, 2288 |
| OliI | CACNNNNGTG | 2 cut(s) | 1280, 1938 |
| PaeI | GCATGC | 1 cut(s) | 2288 |
| PagI | TCATGA | 2 cut(s) | 184, 628 |
| PceI | AGGCCT | 2 cut(s) | 38, 387 |
| PdiI | GCCGGC | 1 cut(s) | 1360 |
| PfeI | GAWTC | 3 cut(s) | 230, 621, 1387 |
| Pfl23II | CGTACG | 1 cut(s) | 690 |
| PflFI | GACNNNGTC | 1 cut(s) | 1060 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 107 |
| PleI | GAGTC | 3 cut(s) | 487, 1271, 1373 |
| PpsI | GAGTC | 3 cut(s) | 487, 1271, 1373 |
| PpuMI | RGGWCCY | 1 cut(s) | 1565 |
| Psp5II | RGGWCCY | 1 cut(s) | 1565 |
| Psp6I | CCWGG | 4 cut(s) | 1076, 1612, 1891, 2140 |
| PspGI | CCWGG | 4 cut(s) | 1076, 1612, 1891, 2140 |
| PspLI | CGTACG | 1 cut(s) | 690 |
| PspN4I | GGNNCC | 6 cut(s) | 159, 730, 1246, 1567, 1742, 1915 |
| PspPI | GGNCC | 7 cut(s) | 509, 836, 1043, 1356, 1565, 1740, 2099 |
| PspPPI | RGGWCCY | 1 cut(s) | 1565 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 2231, 2263 |
| PstI | CTGCAG | 1 cut(s) | 1033 |
| PsuI | RGATCY | 3 cut(s) | 157, 1112, 1481 |
| PsyI | GACNNNGTC | 1 cut(s) | 1060 |
| RsaI | GTAC | 2 cut(s) | 692, 812 |
| RsaNI | GTAC | 2 cut(s) | 691, 811 |
| RseI | CAYNNNNRTG | 3 cut(s) | 897, 1280, 1938 |
| SaqAI | TTAA | 6 cut(s) | 170, 413, 870, 945, 1401, 2193 |
| Sau96I | GGNCC | 7 cut(s) | 509, 836, 1043, 1356, 1565, 1740, 2099 |
| SchI | GAGTC | 3 cut(s) | 488, 1272, 1374 |
| ScrFI | CCNGG | 5 cut(s) | 14, 1078, 1614, 1893, 2142 |
| SduI | GDGCHC | 5 cut(s) | 317, 790, 799, 1505, 1916 |
| SfaNI | GCATC | 6 cut(s) | 206, 462, 676, 847, 1216, 2154 |
| SfcI | CTRYAG | 2 cut(s) | 241, 1029 |
| SinI | GGWCC | 4 cut(s) | 1043, 1565, 1740, 2099 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 897, 1280, 1938 |
| SpeI | ACTAGT | 1 cut(s) | 1765 |
| SphI | GCATGC | 1 cut(s) | 2288 |
| Sse9I | AATT | 8 cut(s) | 409, 653, 940, 1402, 1587, 1661, 2016, 2344 |
| SseBI | AGGCCT | 2 cut(s) | 38, 387 |
| SsiI | CCGC | 6 cut(s) | 222, 356, 720, 1362, 1608, 1641 |
| SspMI | CTAG | 6 cut(s) | 366, 383, 767, 1392, 1560, 1766 |
| StuI | AGGCCT | 2 cut(s) | 38, 387 |
| StyD4I | CCNGG | 5 cut(s) | 12, 1076, 1612, 1891, 2140 |
| StyI | CCWWGG | 3 cut(s) | 382, 766, 1104 |
| TaaI | ACNGT | 8 cut(s) | 1018, 1060, 1090, 1409, 1966, 2086, 2191, 2212 |
| TaiI | ACGT | 3 cut(s) | 550, 1071, 1994 |
| TaqI | TCGA | 8 cut(s) | 30, 482, 914, 1064, 1535, 1788, 2080, 2130 |
| TasI | AATT | 8 cut(s) | 409, 653, 940, 1402, 1587, 1661, 2016, 2344 |
| TatI | WGTACW | 1 cut(s) | 810 |
| TauI | GCSGC | 2 cut(s) | 224, 1610 |
| TfiI | GAWTC | 3 cut(s) | 230, 621, 1387 |
| Tru1I | TTAA | 6 cut(s) | 170, 413, 870, 945, 1401, 2193 |
| Tru9I | TTAA | 6 cut(s) | 170, 413, 870, 945, 1401, 2193 |
| TscAI | CASTG | 7 cut(s) | 715, 859, 934, 1065, 1414, 1456, 1521 |
| TseFI | GTSAC | 3 cut(s) | 929, 1768, 2290 |
| Tsp45I | GTSAC | 3 cut(s) | 929, 1768, 2290 |
| TspDTI | ATGAA | 8 cut(s) | 317, 601, 613, 617, 897, 1414, 1769, 2042 |
| TspGWI | ACGGA | 3 cut(s) | 708, 714, 1328 |
| TspRI | CASTG | 7 cut(s) | 715, 859, 934, 1065, 1414, 1456, 1521 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1060 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 107 |
| VneI | GTGCAC | 1 cut(s) | 786 |
| VpaK11BI | GGWCC | 4 cut(s) | 1043, 1565, 1740, 2099 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2139 |
| XapI | RAATTY | 1 cut(s) | 653 |
| XbaI | TCTAGA | 1 cut(s) | 1559 |
| XceI | RCATGY | 4 cut(s) | 822, 1344, 1988, 2288 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 232, 1238 |
| XmaJI | CCTAGG | 2 cut(s) | 382, 766 |
| XspI | CTAG | 6 cut(s) | 366, 383, 767, 1392, 1560, 1766 |
| Zsp2I | ATGCAT | 1 cut(s) | 420 |