subtilisin-like protease
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 64 |
| AccB1I | GGYRCC | 2 cut(s) | 860, 1520 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1463 |
| AccI | GTMKAC | 1 cut(s) | 318 |
| AccIII | TCCGGA | 1 cut(s) | 1273 |
| AciI | CCGC | 4 cut(s) | 71, 1428, 1592, 1647 |
| AclWI | GGATC | 4 cut(s) | 1151, 1179, 1912, 2210 |
| AcoI | YGGCCR | 3 cut(s) | 554, 879, 1065 |
| AcsI | RAATTY | 3 cut(s) | 635, 743, 2379 |
| AcuI | CTGAAG | 1 cut(s) | 1389 |
| AdeI | CACNNNGTG | 1 cut(s) | 208 |
| AfaI | GTAC | 2 cut(s) | 1402, 2333 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 104, 610, 1280, 1463, 2055, 2225 |
| AflIII | ACRYGT | 1 cut(s) | 2078 |
| AgsI | TTSAA | 8 cut(s) | 486, 574, 688, 724, 1292, 1502, 1573, 2266 |
| AhlI | ACTAGT | 1 cut(s) | 1849 |
| AjnI | CCWGG | 3 cut(s) | 1067, 1218, 1684 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1341, 1373 |
| Alw21I | GWGCWC | 2 cut(s) | 679, 1956 |
| Alw26I | GTCTC | 4 cut(s) | 1786, 1830, 2086, 2178 |
| Alw44I | GTGCAC | 1 cut(s) | 1952 |
| AlwI | GGATC | 4 cut(s) | 1151, 1179, 1912, 2210 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 929, 1412 |
| Ama87I | CYCGRG | 2 cut(s) | 1632, 1975 |
| Aor13HI | TCCGGA | 1 cut(s) | 1273 |
| ApaLI | GTGCAC | 1 cut(s) | 1952 |
| ApoI | RAATTY | 3 cut(s) | 635, 743, 2379 |
| AspA2I | CCTAGG | 1 cut(s) | 586 |
| AspLEI | GCGC | 3 cut(s) | 370, 1117, 1684 |
| AspS9I | GGNCC | 7 cut(s) | 68, 82, 590, 832, 1106, 1637, 1874 |
| AsuC2I | CCSGG | 2 cut(s) | 1976, 1977 |
| AsuHPI | GGTGA | 5 cut(s) | 241, 471, 527, 641, 1128 |
| AsuII | TTCGAA | 1 cut(s) | 1580 |
| AsuNHI | GCTAGC | 1 cut(s) | 788 |
| AvaI | CYCGRG | 2 cut(s) | 1632, 1975 |
| AvaII | GGWCC | 3 cut(s) | 1106, 1637, 1874 |
| AvrII | CCTAGG | 1 cut(s) | 586 |
| BaeGI | GKGCMC | 2 cut(s) | 865, 1956 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 1326, 1359, 2220, 2253 |
| BalI | TGGCCA | 3 cut(s) | 556, 881, 1067 |
| BanI | GGYRCC | 2 cut(s) | 860, 1520 |
| BanII | GRGCYC | 1 cut(s) | 133 |
| Bbv12I | GWGCWC | 2 cut(s) | 679, 1956 |
| BccI | CCATC | 9 cut(s) | 307, 512, 607, 686, 959, 1097, 1457, 1759, 1885 |
| BceAI | ACGGC | 1 cut(s) | 703 |
| BciT130I | CCWGG | 3 cut(s) | 1069, 1220, 1686 |
| BciVI | GTATCC | 1 cut(s) | 1482 |
| BclI | TGATCA | 1 cut(s) | 246 |
| BcnI | CCSGG | 2 cut(s) | 1976, 1977 |
| BcoDI | GTCTC | 4 cut(s) | 1786, 1830, 2086, 2178 |
| BcuI | ACTAGT | 1 cut(s) | 1849 |
| BfaI | CTAG | 4 cut(s) | 444, 587, 789, 1850 |
| BfmI | CTRYAG | 2 cut(s) | 795, 1302 |
| BfuAI | ACCTGC | 1 cut(s) | 64 |
| BfuI | GTATCC | 1 cut(s) | 1482 |
| BlnI | CCTAGG | 1 cut(s) | 586 |
| BmcAI | AGTACT | 1 cut(s) | 1402 |
| Bme1390I | CCNGG | 5 cut(s) | 1069, 1220, 1686, 1976, 1977 |
| Bme18I | GGWCC | 3 cut(s) | 1106, 1637, 1874 |
| BmeT110I | CYCGRG | 2 cut(s) | 1632, 1975 |
| BmgT120I | GGNCC | 7 cut(s) | 68, 82, 590, 832, 1106, 1637, 1874 |
| BmiI | GGNNCC | 5 cut(s) | 805, 862, 1051, 1522, 1638 |
| BmrFI | CCNGG | 5 cut(s) | 1069, 1220, 1686, 1976, 1977 |
| BmrI | ACTGGG | 1 cut(s) | 495 |
| BmsI | GCATC | 4 cut(s) | 751, 1103, 1723, 1822 |
| BmtI | GCTAGC | 1 cut(s) | 792 |
| BmuI | ACTGGG | 1 cut(s) | 495 |
| BpmI | CTGGAG | 2 cut(s) | 1302, 1812 |
| Bpu10I | CCTNAGC | 1 cut(s) | 922 |
| Bpu14I | TTCGAA | 1 cut(s) | 1580 |
| BpuEI | CTTGAG | 2 cut(s) | 1265, 1649 |
| BpuMI | CCSGG | 2 cut(s) | 1976, 1977 |
| Bsa29I | ATCGAT | 1 cut(s) | 1763 |
| BsaBI | GATNNNNATC | 1 cut(s) | 258 |
| BsaI | GGTCTC | 3 cut(s) | 1830, 2086, 2178 |
| BsaWI | WCCGGW | 2 cut(s) | 626, 1273 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 960, 990, 1858, 1888 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 104, 610, 1280, 1463, 2055, 2225 |
| Bse1I | ACTGG | 5 cut(s) | 501, 593, 611, 1051, 1826 |
| Bse3DI | GCAATG | 3 cut(s) | 647, 652, 1038 |
| Bse8I | GATNNNNATC | 1 cut(s) | 258 |
| BseAI | TCCGGA | 1 cut(s) | 1273 |
| BseBI | CCWGG | 3 cut(s) | 1069, 1220, 1686 |
| BseCI | ATCGAT | 1 cut(s) | 1763 |
| BseGI | GGATG | 6 cut(s) | 31, 599, 1024, 1030, 1738, 1813 |
| BseJI | GATNNNNATC | 1 cut(s) | 258 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 104, 610, 1280, 1463, 2055, 2225 |
| BseMI | GCAATG | 3 cut(s) | 647, 652, 1038 |
| BseMII | CTCAG | 8 cut(s) | 106, 488, 558, 696, 936, 1767, 1797, 2155 |
| BseNI | ACTGG | 5 cut(s) | 501, 593, 611, 1051, 1826 |
| BseRI | GAGGAG | 4 cut(s) | 462, 1764, 1790, 1899 |
| BseSI | GKGCMC | 2 cut(s) | 865, 1956 |
| BsgI | GTGCAG | 3 cut(s) | 405, 1110, 2019 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1381 |
| BshNI | GGYRCC | 2 cut(s) | 860, 1520 |
| BshVI | ATCGAT | 1 cut(s) | 1763 |
| BsiEI | CGRYCG | 1 cut(s) | 1381 |
| BsiHKAI | GWGCWC | 2 cut(s) | 679, 1956 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1632, 1975 |
| BsiSI | CCGG | 3 cut(s) | 627, 1274, 1976 |
| BslFI | GGGAC | 4 cut(s) | 736, 859, 1650, 2067 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 104, 610, 1280, 1463, 2055, 2225 |
| BsmAI | GTCTC | 4 cut(s) | 1786, 1830, 2086, 2178 |
| BsmFI | GGGAC | 4 cut(s) | 736, 859, 1650, 2067 |
| BsmI | GAATGC | 2 cut(s) | 1720, 1966 |
| Bso31I | GGTCTC | 3 cut(s) | 1830, 2086, 2178 |
| BsoBI | CYCGRG | 2 cut(s) | 1632, 1975 |
| Bsp119I | TTCGAA | 1 cut(s) | 1580 |
| Bsp1286I | GDGCHC | 4 cut(s) | 133, 679, 865, 1956 |
| Bsp13I | TCCGGA | 1 cut(s) | 1273 |
| Bsp143I | GATC | 7 cut(s) | 246, 1143, 1171, 1544, 1841, 1904, 2215 |
| Bsp19I | CCATGG | 2 cut(s) | 1338, 2028 |
| BspACI | CCGC | 4 cut(s) | 71, 1428, 1592, 1647 |
| BspCNI | CTCAG | 8 cut(s) | 105, 487, 557, 697, 935, 1766, 1796, 2154 |
| BspDI | ATCGAT | 1 cut(s) | 1763 |
| BspEI | TCCGGA | 1 cut(s) | 1273 |
| BspHI | TCATGA | 1 cut(s) | 262 |
| BspLI | GGNNCC | 5 cut(s) | 805, 862, 1051, 1522, 1638 |
| BspMAI | CTGCAG | 1 cut(s) | 799 |
| BspMI | ACCTGC | 1 cut(s) | 64 |
| BspOI | GCTAGC | 1 cut(s) | 792 |
| BspPI | GGATC | 4 cut(s) | 1151, 1179, 1912, 2210 |
| BspT104I | TTCGAA | 1 cut(s) | 1580 |
| BspT107I | GGYRCC | 2 cut(s) | 860, 1520 |
| BspTNI | GGTCTC | 3 cut(s) | 1830, 2086, 2178 |
| BsrDI | GCAATG | 3 cut(s) | 647, 652, 1038 |
| BsrI | ACTGG | 5 cut(s) | 501, 593, 611, 1051, 1826 |
| BssMI | GATC | 7 cut(s) | 246, 1143, 1171, 1544, 1841, 1904, 2215 |
| BssNAI | GTATAC | 1 cut(s) | 319 |
| BssT1I | CCWWGG | 6 cut(s) | 586, 835, 1139, 1338, 1611, 2028 |
| Bst1107I | GTATAC | 1 cut(s) | 319 |
| Bst2UI | CCWGG | 3 cut(s) | 1069, 1220, 1686 |
| BstBI | TTCGAA | 1 cut(s) | 1580 |
| BstC8I | GCNNGC | 8 cut(s) | 273, 372, 790, 1162, 1426, 1594, 1598, 2370 |
| BstDEI | CTNAG | 8 cut(s) | 92, 474, 544, 705, 922, 1753, 1783, 2141 |
| BstDSI | CCRYGG | 4 cut(s) | 1167, 1338, 2028, 2271 |
| BstF5I | GGATG | 6 cut(s) | 31, 599, 1024, 1030, 1738, 1813 |
| BstHHI | GCGC | 3 cut(s) | 370, 1117, 1684 |
| BstKTI | GATC | 7 cut(s) | 249, 1146, 1174, 1547, 1844, 1907, 2218 |
| BstMAI | GTCTC | 4 cut(s) | 1786, 1830, 2086, 2178 |
| BstMBI | GATC | 7 cut(s) | 246, 1143, 1171, 1544, 1841, 1904, 2215 |
| BstMCI | CGRYCG | 1 cut(s) | 1381 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 794, 869, 878, 1434, 1616, 1655 |
| BstNI | CCWGG | 3 cut(s) | 1069, 1220, 1686 |
| BstNSI | RCATGY | 2 cut(s) | 2082, 2372 |
| BstSCI | CCNGG | 5 cut(s) | 1067, 1218, 1684, 1974, 1975 |
| BstSFI | CTRYAG | 2 cut(s) | 795, 1302 |
| BstSLI | GKGCMC | 2 cut(s) | 865, 1956 |
| BstX2I | RGATCY | 1 cut(s) | 1904 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 606, 2257 |
| BstYI | RGATCY | 1 cut(s) | 1904 |
| BstZ17I | GTATAC | 1 cut(s) | 319 |
| Bsu15I | ATCGAT | 1 cut(s) | 1763 |
| BsuI | GTATCC | 1 cut(s) | 1482 |
| BsuTUI | ATCGAT | 1 cut(s) | 1763 |
| BtgI | CCRYGG | 4 cut(s) | 1167, 1338, 2028, 2271 |
| BtsCI | GGATG | 6 cut(s) | 31, 599, 1024, 1030, 1738, 1813 |
| BtsI | GCAGTG | 4 cut(s) | 783, 792, 1715, 1808 |
| BtsIMutI | CAGTG | 5 cut(s) | 604, 783, 792, 1715, 1808 |
| BveI | ACCTGC | 1 cut(s) | 64 |
| Cac8I | GCNNGC | 8 cut(s) | 273, 372, 790, 1162, 1426, 1594, 1598, 2370 |
| CaiI | CAGNNNCTG | 2 cut(s) | 929, 1412 |
| CciI | TCATGA | 1 cut(s) | 262 |
| CfoI | GCGC | 3 cut(s) | 370, 1117, 1684 |
| Cfr13I | GGNCC | 7 cut(s) | 68, 82, 590, 832, 1106, 1637, 1874 |
| Cfr9I | CCCGGG | 1 cut(s) | 1975 |
| ClaI | ATCGAT | 1 cut(s) | 1763 |
| Csp6I | GTAC | 2 cut(s) | 1401, 2332 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 265, 300 |
| CviQI | GTAC | 2 cut(s) | 1401, 2332 |
| DdeI | CTNAG | 8 cut(s) | 92, 474, 544, 705, 922, 1753, 1783, 2141 |
| DpnI | GATC | 7 cut(s) | 248, 1145, 1173, 1546, 1843, 1906, 2217 |
| DpnII | GATC | 7 cut(s) | 246, 1143, 1171, 1544, 1841, 1904, 2215 |
| DraIII | CACNNNGTG | 1 cut(s) | 208 |
| EaeI | YGGCCR | 3 cut(s) | 554, 879, 1065 |
| Eco130I | CCWWGG | 6 cut(s) | 586, 835, 1139, 1338, 1611, 2028 |
| Eco147I | AGGCCT | 1 cut(s) | 585 |
| Eco24I | GRGCYC | 1 cut(s) | 133 |
| Eco31I | GGTCTC | 3 cut(s) | 1830, 2086, 2178 |
| Eco32I | GATATC | 2 cut(s) | 6, 1556 |
| Eco47I | GGWCC | 3 cut(s) | 1106, 1637, 1874 |
| Eco57I | CTGAAG | 1 cut(s) | 1389 |
| Eco88I | CYCGRG | 2 cut(s) | 1632, 1975 |
| EcoO109I | RGGNCCY | 1 cut(s) | 832 |
| EcoRI | GAATTC | 2 cut(s) | 743, 2379 |
| EcoRII | CCWGG | 3 cut(s) | 1067, 1218, 1684 |
| EcoRV | GATATC | 2 cut(s) | 6, 1556 |
| EcoT14I | CCWWGG | 6 cut(s) | 586, 835, 1139, 1338, 1611, 2028 |
| EcoT38I | GRGCYC | 1 cut(s) | 133 |
| ErhI | CCWWGG | 6 cut(s) | 586, 835, 1139, 1338, 1611, 2028 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 178, 210 |
| FaqI | GGGAC | 4 cut(s) | 736, 859, 1650, 2067 |
| FauI | CCCGC | 1 cut(s) | 1585 |
| FbaI | TGATCA | 1 cut(s) | 246 |
| FblI | GTMKAC | 1 cut(s) | 318 |
| FokI | GGATG | 6 cut(s) | 38, 586, 1031, 1037, 1745, 1800 |
| FriOI | GRGCYC | 1 cut(s) | 133 |
| FspBI | CTAG | 4 cut(s) | 444, 587, 789, 1850 |
| GlaI | GCGC | 3 cut(s) | 369, 1116, 1683 |
| GsuI | CTGGAG | 2 cut(s) | 1302, 1812 |
| HapII | CCGG | 3 cut(s) | 627, 1274, 1976 |
| HhaI | GCGC | 3 cut(s) | 370, 1117, 1684 |
| Hin6I | GCGC | 3 cut(s) | 368, 1115, 1682 |
| HinP1I | GCGC | 3 cut(s) | 368, 1115, 1682 |
| HincII | GTYRAC | 2 cut(s) | 1507, 1920 |
| HindII | GTYRAC | 2 cut(s) | 1507, 1920 |
| HindIII | AAGCTT | 4 cut(s) | 295, 356, 942, 1261 |
| HinfI | GANTC | 8 cut(s) | 254, 408, 542, 560, 623, 1277, 1316, 1453 |
| HpaII | CCGG | 3 cut(s) | 627, 1274, 1976 |
| HphI | GGTGA | 5 cut(s) | 241, 471, 527, 641, 1128 |
| Hpy166II | GTNNAC | 8 cut(s) | 202, 319, 1236, 1507, 1693, 1886, 1920, 1954 |
| Hpy188I | TCNGA | 8 cut(s) | 95, 307, 733, 1018, 1128, 1544, 2144, 2280 |
| Hpy8I | GTNNAC | 8 cut(s) | 202, 319, 1236, 1507, 1693, 1886, 1920, 1954 |
| HpyAV | CCTTC | 6 cut(s) | 492, 575, 604, 1119, 2106, 2130 |
| HpyCH4IV | ACGT | 2 cut(s) | 679, 1030 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 794, 869, 878, 1434, 1616, 1655 |
| HpyF3I | CTNAG | 8 cut(s) | 92, 474, 544, 705, 922, 1753, 1783, 2141 |
| HpySE526I | ACGT | 2 cut(s) | 679, 1030 |
| HspAI | GCGC | 3 cut(s) | 368, 1115, 1682 |
| Kpn2I | TCCGGA | 1 cut(s) | 1273 |
| Ksp22I | TGATCA | 1 cut(s) | 246 |
| Kzo9I | GATC | 7 cut(s) | 246, 1143, 1171, 1544, 1841, 1904, 2215 |
| LmnI | GCTCC | 3 cut(s) | 674, 926, 1055 |
| LweI | GCATC | 4 cut(s) | 751, 1103, 1723, 1822 |
| MaeI | CTAG | 4 cut(s) | 444, 587, 789, 1850 |
| MaeII | ACGT | 2 cut(s) | 679, 1030 |
| MaeIII | GTNAC | 9 cut(s) | 323, 477, 811, 824, 981, 1393, 1852, 2158, 2170 |
| MalI | GATC | 7 cut(s) | 248, 1145, 1173, 1546, 1843, 1906, 2217 |
| MboI | GATC | 7 cut(s) | 246, 1143, 1171, 1544, 1841, 1904, 2215 |
| MboII | GAAGA | 2 cut(s) | 488, 590 |
| MfeI | CAATTG | 2 cut(s) | 1587, 2211 |
| MflI | RGATCY | 1 cut(s) | 1904 |
| MhlI | GDGCHC | 4 cut(s) | 133, 679, 865, 1956 |
| MlsI | TGGCCA | 3 cut(s) | 556, 881, 1067 |
| MluNI | TGGCCA | 3 cut(s) | 556, 881, 1067 |
| MlyI | GAGTC | 4 cut(s) | 417, 536, 569, 1286 |
| MmeI | TCCRAC | 2 cut(s) | 265, 1481 |
| Mox20I | TGGCCA | 3 cut(s) | 556, 881, 1067 |
| MroI | TCCGGA | 1 cut(s) | 1273 |
| MscI | TGGCCA | 3 cut(s) | 556, 881, 1067 |
| MseI | TTAA | 4 cut(s) | 185, 639, 1329, 2112 |
| MslI | CAYNNNNRTG | 2 cut(s) | 771, 1959 |
| Msp20I | TGGCCA | 3 cut(s) | 556, 881, 1067 |
| MspA1I | CMGCKG | 2 cut(s) | 800, 1409 |
| MspI | CCGG | 3 cut(s) | 627, 1274, 1976 |
| MspR9I | CCNGG | 5 cut(s) | 1069, 1220, 1686, 1976, 1977 |
| MunI | CAATTG | 2 cut(s) | 1587, 2211 |
| Mva1269I | GAATGC | 2 cut(s) | 1720, 1966 |
| MvaI | CCWGG | 3 cut(s) | 1069, 1220, 1686 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 794, 869, 878, 1434, 1616, 1655 |
| NciI | CCSGG | 2 cut(s) | 1976, 1977 |
| NcoI | CCATGG | 2 cut(s) | 1338, 2028 |
| NdeII | GATC | 7 cut(s) | 246, 1143, 1171, 1544, 1841, 1904, 2215 |
| NheI | GCTAGC | 1 cut(s) | 788 |
| NlaIV | GGNNCC | 5 cut(s) | 805, 862, 1051, 1522, 1638 |
| NmeAIII | GCCGAG | 1 cut(s) | 1924 |
| NmuCI | GTSAC | 5 cut(s) | 477, 811, 981, 1852, 2170 |
| NspI | RCATGY | 2 cut(s) | 2082, 2372 |
| NspV | TTCGAA | 1 cut(s) | 1580 |
| PaeI | GCATGC | 1 cut(s) | 2372 |
| PagI | TCATGA | 1 cut(s) | 262 |
| PceI | AGGCCT | 1 cut(s) | 585 |
| PciI | ACATGT | 1 cut(s) | 2078 |
| PctI | GAATGC | 2 cut(s) | 1720, 1966 |
| PfeI | GAWTC | 4 cut(s) | 254, 623, 1316, 1453 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1463 |
| PleI | GAGTC | 4 cut(s) | 416, 536, 568, 1285 |
| PpsI | GAGTC | 4 cut(s) | 416, 536, 568, 1285 |
| PscI | ACATGT | 1 cut(s) | 2078 |
| Psp6I | CCWGG | 3 cut(s) | 1067, 1218, 1684 |
| PspGI | CCWGG | 3 cut(s) | 1067, 1218, 1684 |
| PspN4I | GGNNCC | 5 cut(s) | 805, 862, 1051, 1522, 1638 |
| PspPI | GGNCC | 7 cut(s) | 68, 82, 590, 832, 1106, 1637, 1874 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 2315, 2347 |
| PstI | CTGCAG | 1 cut(s) | 799 |
| PstNI | CAGNNNCTG | 2 cut(s) | 929, 1412 |
| PsuI | RGATCY | 1 cut(s) | 1904 |
| PvuII | CAGCTG | 2 cut(s) | 800, 1409 |
| RsaI | GTAC | 2 cut(s) | 1402, 2333 |
| RsaNI | GTAC | 2 cut(s) | 1401, 2332 |
| RseI | CAYNNNNRTG | 2 cut(s) | 771, 1959 |
| SaqAI | TTAA | 4 cut(s) | 185, 639, 1329, 2112 |
| Sau3AI | GATC | 7 cut(s) | 246, 1143, 1171, 1544, 1841, 1904, 2215 |
| Sau96I | GGNCC | 7 cut(s) | 68, 82, 590, 832, 1106, 1637, 1874 |
| ScaI | AGTACT | 1 cut(s) | 1402 |
| SchI | GAGTC | 4 cut(s) | 417, 536, 569, 1286 |
| ScrFI | CCNGG | 5 cut(s) | 1069, 1220, 1686, 1976, 1977 |
| SduI | GDGCHC | 4 cut(s) | 133, 679, 865, 1956 |
| SfaNI | GCATC | 4 cut(s) | 751, 1103, 1723, 1822 |
| SfcI | CTRYAG | 2 cut(s) | 795, 1302 |
| SfuI | TTCGAA | 1 cut(s) | 1580 |
| SinI | GGWCC | 3 cut(s) | 1106, 1637, 1874 |
| SmaI | CCCGGG | 1 cut(s) | 1977 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 771, 1959 |
| SmlI | CTYRAG | 2 cut(s) | 1244, 1664 |
| SmoI | CTYRAG | 2 cut(s) | 1244, 1664 |
| SpeI | ACTAGT | 1 cut(s) | 1849 |
| SphI | GCATGC | 1 cut(s) | 2372 |
| SseBI | AGGCCT | 1 cut(s) | 585 |
| SsiI | CCGC | 4 cut(s) | 71, 1428, 1592, 1647 |
| SspI | AATATT | 2 cut(s) | 493, 1605 |
| SspMI | CTAG | 4 cut(s) | 444, 587, 789, 1850 |
| StuI | AGGCCT | 1 cut(s) | 585 |
| StyD4I | CCNGG | 5 cut(s) | 1067, 1218, 1684, 1974, 1975 |
| StyI | CCWWGG | 6 cut(s) | 586, 835, 1139, 1338, 1611, 2028 |
| TaiI | ACGT | 2 cut(s) | 682, 1033 |
| TaqI | TCGA | 9 cut(s) | 406, 874, 989, 1174, 1580, 1763, 1840, 1932, 2292 |
| TaqII | GACCGA | 1 cut(s) | 1367 |
| TatI | WGTACW | 1 cut(s) | 1400 |
| TauI | GCSGC | 1 cut(s) | 73 |
| TfiI | GAWTC | 4 cut(s) | 254, 623, 1316, 1453 |
| Tru1I | TTAA | 4 cut(s) | 185, 639, 1329, 2112 |
| Tru9I | TTAA | 4 cut(s) | 185, 639, 1329, 2112 |
| TscAI | CASTG | 5 cut(s) | 611, 790, 799, 1722, 1815 |
| TseFI | GTSAC | 5 cut(s) | 477, 811, 981, 1852, 2170 |
| Tsp45I | GTSAC | 5 cut(s) | 477, 811, 981, 1852, 2170 |
| TspGWI | ACGGA | 3 cut(s) | 377, 1184, 2185 |
| TspMI | CCCGGG | 1 cut(s) | 1975 |
| TspRI | CASTG | 5 cut(s) | 611, 790, 799, 1722, 1815 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1463 |
| VneI | GTGCAC | 1 cut(s) | 1952 |
| VpaK11BI | GGWCC | 3 cut(s) | 1106, 1637, 1874 |
| XapI | RAATTY | 3 cut(s) | 635, 743, 2379 |
| XceI | RCATGY | 2 cut(s) | 2082, 2372 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 508 |
| XmaI | CCCGGG | 1 cut(s) | 1975 |
| XmaJI | CCTAGG | 1 cut(s) | 586 |
| XmiI | GTMKAC | 1 cut(s) | 318 |
| XspI | CTAG | 4 cut(s) | 444, 587, 789, 1850 |
| ZrmI | AGTACT | 1 cut(s) | 1402 |