AT3G04710

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002248
Physical Location & Seq
Forward (+)
1276668 .. 1281170
4503 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G04710.2

Sequence Viewer

Length: 1371 bp
ATGGCTCCTGATGCTTCTACTGCTCTTGCAGCGAGGGAGAAAGTTCAGCAAATCCTGAATGCAGCTTGTACCGGAAATCTCGAATTCCTTAAGAATGTTGCTAAGCAGCTTGATGAAGGAAAAGACTTGACGAAAACTGTGGAGAGTATTAAAGACGCAAACAAACGTGGAGCGCTTCACTTCGCTGCGAGAGAAGGACAAACTGAAATATGCAGATATCTTTTGGAAGAACTGAAACTCAATGCTGATGCAAAAGATGAAACTGGTGATACTCCGCTTGTTCATGCTGCGAGGCAAGGACAGATTGAAACGGTTAAGTATCTTTTAGAGCAAGGAGCTGATCCCAATATAGCTAGTGAACTAGGAGCCACAGCTTTGCATCATGCGGCTGGGACAGGAGAAATCGAGTTGCTAAAGGAATTGCTCTCTAGAGGTGTTCCTGTTGATTCTGAAAGTGAATCTGGCACGCCATTAATCTGGGCTGCTGGCCACGACCAAAAAAATGCTGTGGAAGTCTTGTTAGAACACAATGCCAATCCTAATGCTGAGACTGAAGATAATATCACACCGTTATTATCTGCAGTGGCAGCGGGTTCTCTGTCATGCTTAGAGTTGTTGGTCAAGGCAGGTGCCAAAGCTAACGTTTTTGCTGGTGGTGCAACTCCATTGCACATTGCGGCTGATATTGGAAACCTTGAGTTAATTAACTGTTTACTTAAAGCCGGGGCTGATCCGAATCAGAAAGATGAGGAGGGTAATAGGCCATTAGAAGTTGCAGCTGCTAGAGACAACAGAAAGGTTGTTGAGATCCTCTTCCCATTGACAACAAAACCAGAAACTGTTTCGGACTGGACAGTAGATGGAATTCTCGCTCACATGGAATCAAACAAAGAACAAGAGGAAAACTCAAACAAGGCGAAATCCCAAGAGACTGGGATCAAGAAAGATCTTCCTGTGGTCTCCCCAGAAGCAAAGGCAAAAGCCGCAGAAGCAAAAGCCAGAGGACAAGACGCCTTTCACAGAAAGGATTTTCAGATGGCTATTGATGCTTATACACAAGCAATTGATTTTGACCCAACGGATCATACCTTATTCTCAAACCGAAGCCTCTGCTGGTTGCGACTAGGACAAGCTGAGCATGCCTTATCTGATGCTAAAGCCTGCAGAGAACTAAACCCAGATTGGCCTAAAGGTTGCTTCAGAGAAGGCGCTGCTCTGCGTTTGCTACAGCGGTTTGACGAGGCAGCCAATGCTTTCTACGAGGGAGTATTGCTTAGCCCTGAAAGCAAAGAGCTCATTGATGCTTTCAGAGAAGCTGTAGATGCCGGAAGGAAGTTTCACGGCAAGGGTGAGGTTAAGGCCAAATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 Ank_5 DUF1685 TPR_17 TPR_8

Protein Analysis

456

Amino Acids

49.26

Weight (kDa)

5.15

Isoelectric Point (pI)

34.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 21 - 117 3.2e-17 Ankyrin repeats (3 copies)
Ank_4 PF13637 59 - 109 5e-09 Ankyrin repeats (many copies)
Ank PF00023 89 - 118 2e-07 Ankyrin repeat
Ank_4 PF13637 93 - 139 1.6e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 119 - 183 1.8e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 192 - 272 6.4e-12 Ankyrin repeats (3 copies)
Ank PF00023 219 - 249 4.2e-08 Ankyrin repeat
TPR_BSK1_C PF25575 325 - 398 3.6e-15 Serine/threonine-protein kinase BSK1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 617
Acc36I ACCTGC 1 cut(s) 617
AccB1I GGYRCC 1 cut(s) 629
AciI CCGC 6 cut(s) 275, 386, 590, 677, 984, 1231
AclI AACGTT 1 cut(s) 642
AclWI GGATC 5 cut(s) 335, 725, 802, 944, 1089
AcoI YGGCCR 1 cut(s) 487
AcsI RAATTY 2 cut(s) 83, 864
AcuI CTGAAG 2 cut(s) 573, 1183
AcyI GRCGYC 1 cut(s) 1011
AfaI GTAC 1 cut(s) 70
AfeI AGCGCT 1 cut(s) 174
AflII CTTAAG 1 cut(s) 89
AgsI TTSAA 1 cut(s) 308
Alw21I GWGCWC 1 cut(s) 1296
Alw26I GTCTC 4 cut(s) 542, 780, 923, 964
AlwI GGATC 5 cut(s) 335, 725, 802, 944, 1089
AlwNI CAGNNNCTG 1 cut(s) 839
Aor51HI AGCGCT 1 cut(s) 174
AoxI GGCC 4 cut(s) 487, 761, 1184, 1359
ApoI RAATTY 2 cut(s) 83, 864
AseI ATTAAT 1 cut(s) 473
AspLEI GCGC 2 cut(s) 175, 1211
AsuC2I CCSGG 1 cut(s) 724
AsuHPI GGTGA 2 cut(s) 278, 1361
BalI TGGCCA 1 cut(s) 489
BanI GGYRCC 1 cut(s) 629
BanII GRGCYC 1 cut(s) 1296
Bbv12I GWGCWC 1 cut(s) 1296
BccI CCATC 2 cut(s) 854, 1030
BceAI ACGGC 1 cut(s) 1357
BcgI CGANNNNNNTGC 2 cut(s) 1092, 1126
BcnI CCSGG 1 cut(s) 724
BcoDI GTCTC 4 cut(s) 542, 780, 923, 964
BfaI CTAG 5 cut(s) 354, 362, 429, 783, 1124
BfmI CTRYAG 4 cut(s) 579, 1162, 1226, 1317
BfoI RGCGCY 2 cut(s) 176, 1212
BfrI CTTAAG 1 cut(s) 89
BfuAI ACCTGC 1 cut(s) 617
BglII AGATCT 1 cut(s) 946
BlpI GCTNAGC 3 cut(s) 102, 1134, 1274
Bme1390I CCNGG 1 cut(s) 724
BmiI GGNNCC 3 cut(s) 6, 367, 631
BmrFI CCNGG 1 cut(s) 724
BmrI ACTGGG 1 cut(s) 942
BmsI GCATC 6 cut(s) 238, 388, 1036, 1141, 1291, 1312
BmuI ACTGGG 1 cut(s) 942
BplI GAGNNNNNCTC 2 cut(s) 890, 922
Bpu1102I GCTNAGC 3 cut(s) 102, 1134, 1274
BpuEI CTTGAG 1 cut(s) 716
BpuMI CCSGG 1 cut(s) 724
BsaBI GATNNNNATC 1 cut(s) 735
BsaHI GRCGYC 1 cut(s) 1011
BsaI GGTCTC 1 cut(s) 964
BsaJI CCNNGG 1 cut(s) 723
BsaWI WCCGGW 1 cut(s) 71
Bse1I ACTGG 3 cut(s) 268, 854, 937
Bse3DI GCAATG 2 cut(s) 665, 672
Bse8I GATNNNNATC 1 cut(s) 735
BseDI CCNNGG 1 cut(s) 723
BseJI GATNNNNATC 1 cut(s) 735
BseMI GCAATG 2 cut(s) 665, 672
BseMII CTCAG 2 cut(s) 537, 1125
BseNI ACTGG 3 cut(s) 268, 854, 937
BseRI GAGGAG 1 cut(s) 764
BseYI CCCAGC 1 cut(s) 389
BshFI GGCC 4 cut(s) 489, 763, 1186, 1361
BshNI GGYRCC 1 cut(s) 629
BsiHKAI GWGCWC 1 cut(s) 1296
BsiSI CCGG 3 cut(s) 72, 723, 1326
BslFI GGGAC 1 cut(s) 406
BsmAI GTCTC 4 cut(s) 542, 780, 923, 964
BsmFI GGGAC 1 cut(s) 406
BsmI GAATGC 1 cut(s) 64
BsnI GGCC 4 cut(s) 489, 763, 1186, 1361
Bso31I GGTCTC 1 cut(s) 964
Bsp1286I GDGCHC 1 cut(s) 1296
Bsp143I GATC 6 cut(s) 340, 730, 807, 936, 946, 1081
Bsp1720I GCTNAGC 3 cut(s) 102, 1134, 1274
BspACI CCGC 6 cut(s) 275, 386, 590, 677, 984, 1231
BspANI GGCC 4 cut(s) 489, 763, 1186, 1361
BspCNI CTCAG 2 cut(s) 538, 1126
BspLI GGNNCC 3 cut(s) 6, 367, 631
BspMAI CTGCAG 2 cut(s) 583, 1166
BspMI ACCTGC 1 cut(s) 617
BspPI GGATC 5 cut(s) 335, 725, 802, 944, 1089
BspT107I GGYRCC 1 cut(s) 629
BspTI CTTAAG 1 cut(s) 89
BspTNI GGTCTC 1 cut(s) 964
BsrDI GCAATG 2 cut(s) 665, 672
BsrI ACTGG 3 cut(s) 268, 854, 937
BssECI CCNNGG 1 cut(s) 723
BssMI GATC 6 cut(s) 340, 730, 807, 936, 946, 1081
BssNI GRCGYC 1 cut(s) 1011
Bst4CI ACNGT 6 cut(s) 139, 313, 570, 710, 841, 856
Bst6I CTCTTC 1 cut(s) 818
BstACI GRCGYC 1 cut(s) 1011
BstAFI CTTAAG 1 cut(s) 89
BstAPI GCANNNNNTGC 1 cut(s) 1250
BstC8I GCNNGC 4 cut(s) 467, 487, 1140, 1162
BstDEI CTNAG 5 cut(s) 102, 546, 607, 1134, 1274
BstH2I RGCGCY 2 cut(s) 176, 1212
BstHHI GCGC 2 cut(s) 175, 1211
BstKTI GATC 6 cut(s) 343, 733, 810, 939, 949, 1084
BstMAI GTCTC 4 cut(s) 542, 780, 923, 964
BstMBI GATC 6 cut(s) 340, 730, 807, 936, 946, 1081
BstNSI RCATGY 1 cut(s) 1142
BstSCI CCNGG 1 cut(s) 722
BstSFI CTRYAG 4 cut(s) 579, 1162, 1226, 1317
BstX2I RGATCY 2 cut(s) 807, 946
BstXI CCANNNNNNTGG 2 cut(s) 477, 932
BstYI RGATCY 2 cut(s) 807, 946
BsuRI GGCC 4 cut(s) 489, 763, 1186, 1361
BtsI GCAGTG 1 cut(s) 588
BtsIMutI CAGTG 1 cut(s) 588
BveI ACCTGC 1 cut(s) 617
Cac8I GCNNGC 4 cut(s) 467, 487, 1140, 1162
CaiI CAGNNNCTG 1 cut(s) 839
CfoI GCGC 2 cut(s) 175, 1211
CseI GACGC 2 cut(s) 164, 1019
Csp6I GTAC 1 cut(s) 69
CviAII CATG 5 cut(s) 284, 383, 603, 877, 1139
CviQI GTAC 1 cut(s) 69
DdeI CTNAG 5 cut(s) 102, 546, 607, 1134, 1274
DpnI GATC 6 cut(s) 342, 732, 809, 938, 948, 1083
DpnII GATC 6 cut(s) 340, 730, 807, 936, 946, 1081
EaeI YGGCCR 1 cut(s) 487
Eam1104I CTCTTC 1 cut(s) 818
EarI CTCTTC 1 cut(s) 818
Ecl136II GAGCTC 1 cut(s) 1294
Eco24I GRGCYC 1 cut(s) 1296
Eco31I GGTCTC 1 cut(s) 964
Eco32I GATATC 1 cut(s) 218
Eco47III AGCGCT 1 cut(s) 174
Eco53kI GAGCTC 1 cut(s) 1294
Eco57I CTGAAG 2 cut(s) 573, 1183
EcoICRI GAGCTC 1 cut(s) 1294
EcoRI GAATTC 2 cut(s) 83, 864
EcoRV GATATC 1 cut(s) 218
EcoT38I GRGCYC 1 cut(s) 1296
FaeI CATG 5 cut(s) 287, 386, 606, 880, 1142
FaqI GGGAC 1 cut(s) 406
FatI CATG 5 cut(s) 283, 382, 602, 876, 1138
FauI CCCGC 1 cut(s) 583
FriOI GRGCYC 1 cut(s) 1296
FspBI CTAG 5 cut(s) 354, 362, 429, 783, 1124
GlaI GCGC 2 cut(s) 174, 1210
GsaI CCCAGC 1 cut(s) 393
HaeII RGCGCY 2 cut(s) 176, 1212
HaeIII GGCC 4 cut(s) 489, 763, 1186, 1361
HapII CCGG 3 cut(s) 72, 723, 1326
HgaI GACGC 2 cut(s) 164, 1019
HhaI GCGC 2 cut(s) 175, 1211
Hin1I GRCGYC 1 cut(s) 1011
Hin1II CATG 5 cut(s) 287, 386, 606, 880, 1142
Hin6I GCGC 2 cut(s) 173, 1209
HinP1I GCGC 2 cut(s) 173, 1209
HinfI GANTC 4 cut(s) 446, 458, 736, 881
HpaII CCGG 3 cut(s) 72, 723, 1326
HphI GGTGA 2 cut(s) 278, 1361
Hpy166II GTNNAC 2 cut(s) 359, 713
Hpy188I TCNGA 8 cut(s) 451, 735, 741, 847, 1035, 1150, 1202, 1310
Hpy188III TCNNGA 5 cut(s) 8, 55, 80, 429, 940
Hpy8I GTNNAC 2 cut(s) 359, 713
HpyAV CCTTC 4 cut(s) 110, 188, 1199, 1323
HpyCH4III ACNGT 6 cut(s) 139, 313, 570, 710, 841, 856
HpyCH4IV ACGT 2 cut(s) 166, 642
HpyF3I CTNAG 5 cut(s) 102, 546, 607, 1134, 1274
HpySE526I ACGT 2 cut(s) 166, 642
Hsp92I GRCGYC 1 cut(s) 1011
Hsp92II CATG 5 cut(s) 287, 386, 606, 880, 1142
HspAI GCGC 2 cut(s) 173, 1209
Kzo9I GATC 6 cut(s) 340, 730, 807, 936, 946, 1081
LmnI GCTCC 4 cut(s) 10, 170, 335, 365
LweI GCATC 6 cut(s) 238, 388, 1036, 1141, 1291, 1312
MaeI CTAG 5 cut(s) 354, 362, 429, 783, 1124
MaeII ACGT 2 cut(s) 166, 642
MalI GATC 6 cut(s) 342, 732, 809, 938, 948, 1083
MboI GATC 6 cut(s) 340, 730, 807, 936, 946, 1081
MboII GAAGA 4 cut(s) 239, 566, 805, 941
MfeI CAATTG 1 cut(s) 1062
MflI RGATCY 2 cut(s) 807, 946
MhlI GDGCHC 1 cut(s) 1296
MlsI TGGCCA 1 cut(s) 489
MluCI AATT 5 cut(s) 83, 419, 702, 864, 1062
MluNI TGGCCA 1 cut(s) 489
Mox20I TGGCCA 1 cut(s) 489
MscI TGGCCA 1 cut(s) 489
MseI TTAA 8 cut(s) 90, 150, 315, 473, 701, 705, 717, 1356
Msp20I TGGCCA 1 cut(s) 489
MspA1I CMGCKG 3 cut(s) 590, 779, 1231
MspCI CTTAAG 1 cut(s) 89
MspI CCGG 3 cut(s) 72, 723, 1326
MspR9I CCNGG 1 cut(s) 724
MunI CAATTG 1 cut(s) 1062
Mva1269I GAATGC 1 cut(s) 64
NciI CCSGG 1 cut(s) 724
NdeII GATC 6 cut(s) 340, 730, 807, 936, 946, 1081
NlaIII CATG 5 cut(s) 287, 386, 606, 880, 1142
NlaIV GGNNCC 3 cut(s) 6, 367, 631
NspI RCATGY 1 cut(s) 1142
PacI TTAATTAA 1 cut(s) 705
PaeI GCATGC 1 cut(s) 1142
PaqCI CACCTGC 1 cut(s) 617
PctI GAATGC 1 cut(s) 64
PfeI GAWTC 4 cut(s) 446, 458, 736, 881
PshBI ATTAAT 1 cut(s) 473
Psp124BI GAGCTC 1 cut(s) 1296
Psp1406I AACGTT 1 cut(s) 642
PspFI CCCAGC 1 cut(s) 389
PspN4I GGNNCC 3 cut(s) 6, 367, 631
PstI CTGCAG 2 cut(s) 583, 1166
PstNI CAGNNNCTG 1 cut(s) 839
PsuI RGATCY 2 cut(s) 807, 946
PvuII CAGCTG 1 cut(s) 779
RsaI GTAC 1 cut(s) 70
RsaNI GTAC 1 cut(s) 69
SacI GAGCTC 1 cut(s) 1296
SaqAI TTAA 8 cut(s) 90, 150, 315, 473, 701, 705, 717, 1356
Sau3AI GATC 6 cut(s) 340, 730, 807, 936, 946, 1081
ScrFI CCNGG 1 cut(s) 724
SduI GDGCHC 1 cut(s) 1296
SfaNI GCATC 6 cut(s) 238, 388, 1036, 1141, 1291, 1312
SfcI CTRYAG 4 cut(s) 579, 1162, 1226, 1317
SmlI CTYRAG 2 cut(s) 89, 695
SmoI CTYRAG 2 cut(s) 89, 695
SphI GCATGC 1 cut(s) 1142
Sse9I AATT 5 cut(s) 83, 419, 702, 864, 1062
SsiI CCGC 6 cut(s) 275, 386, 590, 677, 984, 1231
SspMI CTAG 5 cut(s) 354, 362, 429, 783, 1124
SstI GAGCTC 1 cut(s) 1296
StyD4I CCNGG 1 cut(s) 722
TaaI ACNGT 6 cut(s) 139, 313, 570, 710, 841, 856
TaiI ACGT 2 cut(s) 169, 645
TaqI TCGA 2 cut(s) 81, 405
TasI AATT 5 cut(s) 83, 419, 702, 864, 1062
TauI GCSGC 3 cut(s) 389, 680, 986
TfiI GAWTC 4 cut(s) 446, 458, 736, 881
Tru1I TTAA 8 cut(s) 90, 150, 315, 473, 701, 705, 717, 1356
Tru9I TTAA 8 cut(s) 90, 150, 315, 473, 701, 705, 717, 1356
TscAI CASTG 1 cut(s) 588
TspDTI ATGAA 3 cut(s) 129, 272, 273
TspGWI ACGGA 1 cut(s) 1094
TspRI CASTG 1 cut(s) 588
Vha464I CTTAAG 1 cut(s) 89
VspI ATTAAT 1 cut(s) 473
XapI RAATTY 2 cut(s) 83, 864
XbaI TCTAGA 1 cut(s) 428
XceI RCATGY 1 cut(s) 1142
XspI CTAG 5 cut(s) 354, 362, 429, 783, 1124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.