RLG00000015782

ankyrin repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002248
Physical Location & Seq
Reverse (-)
1407043 .. 1409289
2247 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015782

Sequence Viewer

Length: 522 bp
ATGTGCAAGTACTTGCTGGAGGAATTGAAAATTGATGTTGATACCAAAGATGAAGATGGCCATACTGATACTGCCAAACTGCTTCTTGAGTATGGTGCCAATTCGTCTATAGCCAGTGATTTAGGGGCTACAGCTCTTCATCATTCCGCTGGACTAGGAGACATTGAGTTGCTGAGGTACTTAATTTCCAAGTGTGTTGATGTCAATTCACAAAGTGATGCTGGTTCTCCTTTAATTTGGGCTGCTGGTCATGACCAACTAGATGCTGTGAAAGTTCTGTTAGAACACCAGGCTAATCCTAATGTTGAAAGTGATGATGATATTACTCCATTGTTGTCATCTGTGGCAGCTGGTTCACTGGCATGCTTGGAGTTATTGATTCAGGCAGGTGCCAAAGTAAATATGAGTGCTGGTGGAGCGACCCCTTTACACATCGCTGCTGACATTGAGAACTTGGAGATTATAAAATGTTTGTTAAAAGCTGGAGCTGATCCCAGTGTAACTGATGAGGTGAGGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 Ank_5 DUF1685 TPR_17 TPR_8

Protein Analysis

174

Amino Acids

18.18

Weight (kDa)

4.4

Isoelectric Point (pI)

27.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 19 - 103 6.6e-15 Ankyrin repeats (3 copies)
Ank_4 PF13637 78 - 127 1.2e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 100 - 169 8.1e-11 Ankyrin repeats (3 copies)
Ank_4 PF13637 117 - 159 8.7e-07 Ankyrin repeats (many copies)
Ank PF00023 139 - 169 8.3e-08 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 464
AarI CACCTGC 1 cut(s) 377
Acc36I ACCTGC 1 cut(s) 377
AccB1I GGYRCC 2 cut(s) 95, 389
AciI CCGC 1 cut(s) 147
AclWI GGATC 1 cut(s) 485
AcoI YGGCCR 1 cut(s) 58
AdeI CACNNNGTG 1 cut(s) 215
AfaI GTAC 2 cut(s) 11, 179
AgsI TTSAA 2 cut(s) 28, 308
AjnI CCWGG 1 cut(s) 288
AluBI AGCT 4 cut(s) 134, 350, 482, 488
AluI AGCT 4 cut(s) 134, 350, 482, 488
Alw26I GTCTC 1 cut(s) 153
AlwI GGATC 1 cut(s) 485
AoxI GGCC 1 cut(s) 58
ApeKI GCWGC 3 cut(s) 242, 347, 437
BalI TGGCCA 1 cut(s) 60
BanI GGYRCC 2 cut(s) 95, 389
BbvCI CCTCAGC 1 cut(s) 173
BbvI GCAGC 3 cut(s) 229, 359, 424
BccI CCATC 1 cut(s) 50
BciT130I CCWGG 1 cut(s) 290
BcoDI GTCTC 1 cut(s) 153
BfaI CTAG 2 cut(s) 155, 260
BfmI CTRYAG 2 cut(s) 108, 129
BfuAI ACCTGC 1 cut(s) 377
BisI GCNGC 3 cut(s) 243, 348, 438
BlsI GCNGC 3 cut(s) 244, 349, 439
BmcAI AGTACT 1 cut(s) 11
Bme1390I CCNGG 1 cut(s) 290
BmiI GGNNCC 2 cut(s) 97, 391
BmrFI CCNGG 1 cut(s) 290
BmrI ACTGGG 1 cut(s) 489
BmsI GCATC 2 cut(s) 208, 253
BmuI ACTGGG 1 cut(s) 489
BpmI CTGGAG 2 cut(s) 38, 504
Bpu10I CCTNAGC 1 cut(s) 173
BpuEI CTTGAG 1 cut(s) 107
Bse1I ACTGG 3 cut(s) 114, 363, 495
BseBI CCWGG 1 cut(s) 290
BseMII CTCAG 1 cut(s) 164
BseNI ACTGG 3 cut(s) 114, 363, 495
BseXI GCAGC 3 cut(s) 229, 359, 424
BshFI GGCC 1 cut(s) 60
BshNI GGYRCC 2 cut(s) 95, 389
BsmAI GTCTC 1 cut(s) 153
BsnI GGCC 1 cut(s) 60
Bsp143I GATC 1 cut(s) 490
BspACI CCGC 1 cut(s) 147
BspANI GGCC 1 cut(s) 60
BspCNI CTCAG 1 cut(s) 165
BspHI TCATGA 1 cut(s) 250
BspLI GGNNCC 2 cut(s) 97, 391
BspMI ACCTGC 1 cut(s) 377
BspPI GGATC 1 cut(s) 485
BspQI GCTCTTC 1 cut(s) 141
BspT107I GGYRCC 2 cut(s) 95, 389
BsrI ACTGG 3 cut(s) 114, 363, 495
BssMI GATC 1 cut(s) 490
Bst2UI CCWGG 1 cut(s) 290
Bst6I CTCTTC 1 cut(s) 141
BstC8I GCNNGC 1 cut(s) 364
BstDEI CTNAG 1 cut(s) 173
BstKTI GATC 1 cut(s) 493
BstMAI GTCTC 1 cut(s) 153
BstMBI GATC 1 cut(s) 490
BstMWI GCNNNNNNNGC 1 cut(s) 416
BstNI CCWGG 1 cut(s) 290
BstNSI RCATGY 1 cut(s) 366
BstSCI CCNGG 1 cut(s) 288
BstSFI CTRYAG 2 cut(s) 108, 129
BstV1I GCAGC 3 cut(s) 229, 359, 424
BsuRI GGCC 1 cut(s) 60
BtgZI GCGATG 1 cut(s) 418
BtsIMutI CAGTG 3 cut(s) 121, 356, 502
BveI ACCTGC 1 cut(s) 377
Cac8I GCNNGC 1 cut(s) 364
CciI TCATGA 1 cut(s) 250
Csp6I GTAC 2 cut(s) 10, 178
CviAII CATG 2 cut(s) 251, 363
CviQI GTAC 2 cut(s) 10, 178
DdeI CTNAG 1 cut(s) 173
DpnI GATC 1 cut(s) 492
DpnII GATC 1 cut(s) 490
DraIII CACNNNGTG 1 cut(s) 215
EaeI YGGCCR 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 141
EarI CTCTTC 1 cut(s) 141
EcoRII CCWGG 1 cut(s) 288
FaeI CATG 2 cut(s) 254, 366
FaiI YATR 8 cut(s) 63, 93, 110, 252, 364, 404, 464, 520
FatI CATG 2 cut(s) 250, 362
Fnu4HI GCNGC 3 cut(s) 243, 348, 438
Fsp4HI GCNGC 3 cut(s) 243, 348, 438
FspBI CTAG 2 cut(s) 155, 260
GluI GCNGC 3 cut(s) 243, 348, 438
GsuI CTGGAG 2 cut(s) 38, 504
HaeIII GGCC 1 cut(s) 60
Hin1II CATG 2 cut(s) 254, 366
HinfI GANTC 1 cut(s) 379
Hpy166II GTNNAC 1 cut(s) 356
Hpy188III TCNNGA 2 cut(s) 86, 251
Hpy8I GTNNAC 1 cut(s) 356
HpyCH4V TGCA 1 cut(s) 6
HpyF10VI GCNNNNNNNGC 1 cut(s) 416
HpyF3I CTNAG 1 cut(s) 173
Hsp92II CATG 2 cut(s) 254, 366
Kzo9I GATC 1 cut(s) 490
LguI GCTCTTC 1 cut(s) 141
LmnI GCTCC 2 cut(s) 416, 485
Lsp1109I GCAGC 3 cut(s) 229, 359, 424
LweI GCATC 2 cut(s) 208, 253
MaeI CTAG 2 cut(s) 155, 260
MaeIII GTNAC 1 cut(s) 499
MalI GATC 1 cut(s) 492
MboI GATC 1 cut(s) 490
MboII GAAGA 2 cut(s) 65, 128
MlsI TGGCCA 1 cut(s) 60
MluCI AATT 6 cut(s) 23, 30, 100, 183, 205, 234
MluNI TGGCCA 1 cut(s) 60
MnlI CCTC 4 cut(s) 13, 168, 502, 507
Mox20I TGGCCA 1 cut(s) 60
MscI TGGCCA 1 cut(s) 60
MseI TTAA 3 cut(s) 182, 233, 476
MslI CAYNNNNRTG 1 cut(s) 361
Msp20I TGGCCA 1 cut(s) 60
MspA1I CMGCKG 2 cut(s) 149, 350
MspR9I CCNGG 1 cut(s) 290
MvaI CCWGG 1 cut(s) 290
MwoI GCNNNNNNNGC 1 cut(s) 416
NdeII GATC 1 cut(s) 490
NlaIII CATG 2 cut(s) 254, 366
NlaIV GGNNCC 2 cut(s) 97, 391
NspI RCATGY 1 cut(s) 366
PaeI GCATGC 1 cut(s) 366
PagI TCATGA 1 cut(s) 250
PaqCI CACCTGC 1 cut(s) 377
PciSI GCTCTTC 1 cut(s) 141
PfeI GAWTC 1 cut(s) 379
PkrI GCNGC 3 cut(s) 244, 349, 439
PsiI TTATAA 1 cut(s) 464
Psp6I CCWGG 1 cut(s) 288
PspGI CCWGG 1 cut(s) 288
PspN4I GGNNCC 2 cut(s) 97, 391
PvuII CAGCTG 1 cut(s) 350
RsaI GTAC 2 cut(s) 11, 179
RsaNI GTAC 2 cut(s) 10, 178
RseI CAYNNNNRTG 1 cut(s) 361
SapI GCTCTTC 1 cut(s) 141
SaqAI TTAA 3 cut(s) 182, 233, 476
SatI GCNGC 3 cut(s) 243, 348, 438
Sau3AI GATC 1 cut(s) 490
ScaI AGTACT 1 cut(s) 11
ScrFI CCNGG 1 cut(s) 290
SetI ASST 7 cut(s) 136, 179, 352, 391, 484, 490, 513
SfaNI GCATC 2 cut(s) 208, 253
SfcI CTRYAG 2 cut(s) 108, 129
SmiMI CAYNNNNRTG 1 cut(s) 361
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
SphI GCATGC 1 cut(s) 366
Sse9I AATT 6 cut(s) 23, 30, 100, 183, 205, 234
SsiI CCGC 1 cut(s) 147
SspMI CTAG 2 cut(s) 155, 260
StyD4I CCNGG 1 cut(s) 288
TasI AATT 6 cut(s) 23, 30, 100, 183, 205, 234
TatI WGTACW 1 cut(s) 9
TfiI GAWTC 1 cut(s) 379
Tru1I TTAA 3 cut(s) 182, 233, 476
Tru9I TTAA 3 cut(s) 182, 233, 476
TscAI CASTG 3 cut(s) 121, 363, 502
TseI GCWGC 3 cut(s) 242, 347, 437
TspDTI ATGAA 2 cut(s) 66, 128
TspRI CASTG 3 cut(s) 121, 363, 502
XceI RCATGY 1 cut(s) 366
XspI CTAG 2 cut(s) 155, 260
ZrmI AGTACT 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.