RLG00000025714

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002248
Physical Location & Seq
Reverse (-)
47834988 .. 47839436
4449 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025714

Sequence Viewer

Length: 1290 bp
ATGGCCTCTGACGCTTCTGATGCACTTGCAGCTAGGGAAATAGTGCAGCAGTTTCTGAATGCTGCATGTACTGGGGATGTTGATCTCTTAAAGAAGGGCCAGTCTGAGATGTGCAAGTACTTGCTGGAGGAATTGAAACTTGATGTTGATACCAAAGACGAAGATGGCGACACTCCCCTTATTCATGCTGCTCGCCTAGGCCATACTGATACTGCAAAACTGCTTCTTGAGTATGGTGCCAATCCGTCTGTAGCCAGTGATTTAGGGGCTACAGCTCTTCATCATTCCGCTGGACTAGGAAACATTGAGTTGCTGAGGTACTTAATTTCCAAGGGTGTCGATGTCAATTCACAAAGTGATGCTGGTTCTCCCTTAATTTGGGCTGCTGGTCATGACCAACCAGATGCTGTGAAAGTTCTGTTAGAACACCACGCTAATCCTAATGCTGAAAGTGATGATGATATTACTCCATTGTTGTCATCTGTGGCAGCTGGTTCACTGGCATGCTTGGAGTTATTGATTCAGGCAGGTGCCAAAGTAAATATGAGTGCTGGTGGAGCGACCCCTTTACACATTGCTGCTGATATTGGGAACTTGGAGATTATAAAATGTTTGTTAAAAGCTGGAGCTGATCCCAATGTAACTGATGAGGAGGGTTTGAAGCCAATACAGGTTGCAGCTGCAAGTGGGAACCGAAGCGTTGTGGAGATCCTCTTTCCCTTGACATCACAAGTTGAAACCATTTCAAAATGGACAGTTGACGGCATATTTGAGTATATGCAGTCTGAAACAAGCAGACAACAGGAACAACTGCGAAATACTAGGGAACTTGATGTCCCAAAAGACTCTACTTCTCTGAAGAAAGAGCTCCCTGAGGTGACACCTGAGGCAAAGAAGAGAGCCGCAGAAGCAAAATCGAGAGGAGGCGATGCTTTCAGTAGAAAGGATTATCAAGCGGCTGTTGATGCATACACACAGGCAATTGATTTGGACCCAACTGATGCCATTTTTCTTTCCAATCGAAGCCTTTGTTGGCTTCGTCTTGGCCAAGCTGAACATGCCTTAGCTGATGCAAAGGCCTGCAGGGCATTGAAACCAGATTGGCCAAAGGCTTGCTTTCGAGAAGGTGCAGCATTGCGCCTAATGCAGAAGTTTGATGAAGCAGCCAATTGTTTTTATGAGGGTGTGCAGCTCGACCCTGAAAGTAAGGAACTCGTCGATGCTTTCAGGGAAGCGGTTGAAGCTGGAAGGCAGTTCCACAGTAAAGATAAACAAAAACCAAAACCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 Ank_5 DUF1685 TPR_17 TPR_8

Protein Analysis

430

Amino Acids

46.29

Weight (kDa)

4.93

Isoelectric Point (pI)

34.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 31 - 118 3.5e-18 Ankyrin repeats (3 copies)
Ank PF00023 55 - 84 3.1e-07 Ankyrin repeat
Ank_4 PF13637 61 - 109 3.4e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 85 - 150 8.1e-12 Ankyrin repeats (3 copies)
Ank_2 PF12796 122 - 182 6.9e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 163 - 240 9.5e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 164 - 206 6.3e-07 Ankyrin repeats (many copies)
Ank_5 PF13857 173 - 226 1.3e-08 Ankyrin repeats (many copies)
Ank PF00023 186 - 216 3.4e-09 Ankyrin repeat
Ank_3 PF13606 186 - 214 3.5e-06 Ankyrin repeat
TPR_BSK1_C PF25575 297 - 373 2.4e-13 Serine/threonine-protein kinase BSK1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 605
AarI CACCTGC 1 cut(s) 518
Acc36I ACCTGC 1 cut(s) 518
AccB1I GGYRCC 2 cut(s) 236, 530
AciI CCGC 4 cut(s) 288, 903, 956, 1235
AclWI GGATC 2 cut(s) 626, 703
AcoI YGGCCR 2 cut(s) 1045, 1103
AcuI CTGAAG 1 cut(s) 878
AdeI CACNNNGTG 1 cut(s) 356
AfaI GTAC 3 cut(s) 70, 119, 320
AfiI CCNNNNNNNGG 1 cut(s) 378
AgsI TTSAA 6 cut(s) 136, 661, 737, 747, 1093, 1241
AjuI GAANNNNNNNTTGG 2 cut(s) 1015, 1047
AloI GAACNNNNNNTCC 2 cut(s) 819, 851
Alw21I GWGCWC 1 cut(s) 870
AlwI GGATC 2 cut(s) 626, 703
AlwNI CAGNNNCTG 2 cut(s) 55, 407
AoxI GGCC 6 cut(s) 3, 97, 199, 1045, 1077, 1103
ArsI GACNNNNNNTTYG 2 cut(s) 752, 784
AspA2I CCTAGG 1 cut(s) 196
AspLEI GCGC 1 cut(s) 1140
AspS9I GGNCC 2 cut(s) 97, 991
AsuHPI GGTGA 1 cut(s) 889
AvaII GGWCC 1 cut(s) 991
AvrII CCTAGG 1 cut(s) 196
AxyI CCTNAGG 2 cut(s) 873, 885
BalI TGGCCA 2 cut(s) 1047, 1105
BanI GGYRCC 2 cut(s) 236, 530
BanII GRGCYC 1 cut(s) 870
Bbv12I GWGCWC 1 cut(s) 870
BbvCI CCTCAGC 1 cut(s) 314
BccI CCATC 1 cut(s) 158
BceAI ACGGC 1 cut(s) 778
BfaI CTAG 4 cut(s) 33, 197, 296, 822
BfmI CTRYAG 3 cut(s) 249, 270, 1081
BfuAI ACCTGC 1 cut(s) 518
BglI GCCNNNNNGGC 1 cut(s) 1085
BlnI CCTAGG 1 cut(s) 196
BmcAI AGTACT 1 cut(s) 119
Bme18I GGWCC 1 cut(s) 991
BmgT120I GGNCC 2 cut(s) 97, 991
BmiI GGNNCC 4 cut(s) 238, 532, 692, 993
BmrI ACTGGG 1 cut(s) 81
BmsI GCATC 8 cut(s) 10, 349, 394, 919, 955, 991, 1060, 1210
BmuI ACTGGG 1 cut(s) 81
BpmI CTGGAG 2 cut(s) 146, 645
Bpu10I CCTNAGC 2 cut(s) 314, 1063
BpuEI CTTGAG 1 cut(s) 248
BsaBI GATNNNNATC 1 cut(s) 81
BsaJI CCNNGG 2 cut(s) 196, 330
Bsc4I CCNNNNNNNGG 1 cut(s) 378
Bse1I ACTGG 4 cut(s) 76, 100, 255, 504
Bse21I CCTNAGG 2 cut(s) 873, 885
Bse3DI GCAATG 2 cut(s) 573, 1133
Bse8I GATNNNNATC 1 cut(s) 81
BseDI CCNNGG 2 cut(s) 196, 330
BseGI GGATG 1 cut(s) 82
BseJI GATNNNNATC 1 cut(s) 81
BseLI CCNNNNNNNGG 1 cut(s) 378
BseMI GCAATG 2 cut(s) 573, 1133
BseMII CTCAG 4 cut(s) 96, 305, 864, 876
BseNI ACTGG 4 cut(s) 76, 100, 255, 504
BseRI GAGGAG 2 cut(s) 665, 936
BsgI GTGCAG 3 cut(s) 65, 1149, 1208
BshFI GGCC 6 cut(s) 5, 99, 201, 1047, 1079, 1105
BshNI GGYRCC 2 cut(s) 236, 530
BsiHKAI GWGCWC 1 cut(s) 870
BslFI GGGAC 1 cut(s) 821
BslI CCNNNNNNNGG 1 cut(s) 378
BsmFI GGGAC 1 cut(s) 821
BsmI GAATGC 1 cut(s) 64
BsnI GGCC 6 cut(s) 5, 99, 201, 1047, 1079, 1105
Bsp1286I GDGCHC 1 cut(s) 870
Bsp143I GATC 3 cut(s) 82, 631, 708
BspACI CCGC 4 cut(s) 288, 903, 956, 1235
BspANI GGCC 6 cut(s) 5, 99, 201, 1047, 1079, 1105
BspCNI CTCAG 4 cut(s) 97, 306, 865, 877
BspHI TCATGA 1 cut(s) 391
BspLI GGNNCC 4 cut(s) 238, 532, 692, 993
BspMAI CTGCAG 1 cut(s) 1085
BspMI ACCTGC 1 cut(s) 518
BspPI GGATC 2 cut(s) 626, 703
BspQI GCTCTTC 1 cut(s) 282
BspT107I GGYRCC 2 cut(s) 236, 530
BsrDI GCAATG 2 cut(s) 573, 1133
BsrI ACTGG 4 cut(s) 76, 100, 255, 504
BssECI CCNNGG 2 cut(s) 196, 330
BssMI GATC 3 cut(s) 82, 631, 708
BssT1I CCWWGG 2 cut(s) 196, 330
Bst4CI ACNGT 2 cut(s) 757, 1262
Bst6I CTCTTC 2 cut(s) 282, 890
BstC8I GCNNGC 4 cut(s) 193, 505, 1081, 1114
BstDEI CTNAG 6 cut(s) 105, 314, 873, 885, 1063, 1287
BstF5I GGATG 1 cut(s) 82
BstHHI GCGC 1 cut(s) 1140
BstKTI GATC 3 cut(s) 85, 634, 711
BstMBI GATC 3 cut(s) 82, 631, 708
BstNSI RCATGY 3 cut(s) 69, 507, 1061
BstSFI CTRYAG 3 cut(s) 249, 270, 1081
BstX2I RGATCY 1 cut(s) 708
BstYI RGATCY 1 cut(s) 708
Bsu36I CCTNAGG 2 cut(s) 873, 885
BsuRI GGCC 6 cut(s) 5, 99, 201, 1047, 1079, 1105
BtgZI GCGATG 1 cut(s) 942
BtsCI GGATG 1 cut(s) 82
BtsIMutI CAGTG 2 cut(s) 262, 497
BveI ACCTGC 1 cut(s) 518
Cac8I GCNNGC 4 cut(s) 193, 505, 1081, 1114
CaiI CAGNNNCTG 2 cut(s) 55, 407
CciI TCATGA 1 cut(s) 391
CfoI GCGC 1 cut(s) 1140
Cfr13I GGNCC 2 cut(s) 97, 991
CseI GACGC 1 cut(s) 20
Csp6I GTAC 3 cut(s) 69, 118, 319
CviAII CATG 5 cut(s) 66, 185, 392, 504, 1058
CviQI GTAC 3 cut(s) 69, 118, 319
DdeI CTNAG 6 cut(s) 105, 314, 873, 885, 1063, 1287
DpnI GATC 3 cut(s) 84, 633, 710
DpnII GATC 3 cut(s) 82, 631, 708
DraIII CACNNNGTG 1 cut(s) 356
EaeI YGGCCR 2 cut(s) 1045, 1103
Eam1104I CTCTTC 2 cut(s) 282, 890
EarI CTCTTC 2 cut(s) 282, 890
Ecl136II GAGCTC 1 cut(s) 868
Eco130I CCWWGG 2 cut(s) 196, 330
Eco147I AGGCCT 1 cut(s) 1079
Eco24I GRGCYC 1 cut(s) 870
Eco47I GGWCC 1 cut(s) 991
Eco53kI GAGCTC 1 cut(s) 868
Eco57I CTGAAG 1 cut(s) 878
Eco81I CCTNAGG 2 cut(s) 873, 885
EcoICRI GAGCTC 1 cut(s) 868
EcoT14I CCWWGG 2 cut(s) 196, 330
EcoT22I ATGCAT 1 cut(s) 970
EcoT38I GRGCYC 1 cut(s) 870
ErhI CCWWGG 2 cut(s) 196, 330
FaeI CATG 5 cut(s) 69, 188, 395, 507, 1061
FalI AAGNNNNNCTT 2 cut(s) 1100, 1132
FaqI GGGAC 1 cut(s) 821
FatI CATG 5 cut(s) 65, 184, 391, 503, 1057
FokI GGATG 1 cut(s) 89
FriOI GRGCYC 1 cut(s) 870
FspBI CTAG 4 cut(s) 33, 197, 296, 822
GlaI GCGC 1 cut(s) 1139
GsuI CTGGAG 2 cut(s) 146, 645
HaeIII GGCC 6 cut(s) 5, 99, 201, 1047, 1079, 1105
HgaI GACGC 1 cut(s) 20
HhaI GCGC 1 cut(s) 1140
Hin1II CATG 5 cut(s) 69, 188, 395, 507, 1061
Hin6I GCGC 1 cut(s) 1138
HinP1I GCGC 1 cut(s) 1138
HincII GTYRAC 1 cut(s) 760
HindII GTYRAC 1 cut(s) 760
HinfI GANTC 2 cut(s) 520, 845
HphI GGTGA 1 cut(s) 889
Hpy166II GTNNAC 2 cut(s) 497, 760
Hpy188I TCNGA 6 cut(s) 10, 19, 57, 106, 787, 858
Hpy188III TCNNGA 4 cut(s) 227, 392, 918, 1121
Hpy8I GTNNAC 2 cut(s) 497, 760
Hpy99I CGWCG 1 cut(s) 1220
HpyAV CCTTC 3 cut(s) 88, 1118, 1242
HpyCH4III ACNGT 2 cut(s) 757, 1262
HpyF3I CTNAG 6 cut(s) 105, 314, 873, 885, 1063, 1287
Hsp92II CATG 5 cut(s) 69, 188, 395, 507, 1061
HspAI GCGC 1 cut(s) 1138
Kzo9I GATC 3 cut(s) 82, 631, 708
LguI GCTCTTC 1 cut(s) 282
LmnI GCTCC 3 cut(s) 557, 626, 873
LweI GCATC 8 cut(s) 10, 349, 394, 919, 955, 991, 1060, 1210
MaeI CTAG 4 cut(s) 33, 197, 296, 822
MaeIII GTNAC 2 cut(s) 640, 877
MalI GATC 3 cut(s) 84, 633, 710
MboI GATC 3 cut(s) 82, 631, 708
MboII GAAGA 4 cut(s) 173, 269, 871, 907
MfeI CAATTG 2 cut(s) 981, 1168
MflI RGATCY 1 cut(s) 708
MhlI GDGCHC 1 cut(s) 870
MlsI TGGCCA 2 cut(s) 1047, 1105
MluCI AATT 6 cut(s) 131, 324, 346, 375, 981, 1168
MluNI TGGCCA 2 cut(s) 1047, 1105
MlyI GAGTC 1 cut(s) 839
Mox20I TGGCCA 2 cut(s) 1047, 1105
Mph1103I ATGCAT 1 cut(s) 970
MscI TGGCCA 2 cut(s) 1047, 1105
MseI TTAA 4 cut(s) 89, 323, 374, 617
MslI CAYNNNNRTG 1 cut(s) 502
Msp20I TGGCCA 2 cut(s) 1047, 1105
MspA1I CMGCKG 3 cut(s) 290, 491, 680
MunI CAATTG 2 cut(s) 981, 1168
Mva1269I GAATGC 1 cut(s) 64
NdeII GATC 3 cut(s) 82, 631, 708
NlaIII CATG 5 cut(s) 69, 188, 395, 507, 1061
NlaIV GGNNCC 4 cut(s) 238, 532, 692, 993
NmuCI GTSAC 1 cut(s) 877
NsiI ATGCAT 1 cut(s) 970
NspI RCATGY 3 cut(s) 69, 507, 1061
PaeI GCATGC 1 cut(s) 507
PagI TCATGA 1 cut(s) 391
PaqCI CACCTGC 1 cut(s) 518
PceI AGGCCT 1 cut(s) 1079
PciSI GCTCTTC 1 cut(s) 282
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PctI GAATGC 1 cut(s) 64
PfeI GAWTC 1 cut(s) 520
PleI GAGTC 1 cut(s) 839
PpsI GAGTC 1 cut(s) 839
PsiI TTATAA 1 cut(s) 605
Psp124BI GAGCTC 1 cut(s) 870
PspN4I GGNNCC 4 cut(s) 238, 532, 692, 993
PspPI GGNCC 2 cut(s) 97, 991
PstI CTGCAG 1 cut(s) 1085
PstNI CAGNNNCTG 2 cut(s) 55, 407
PsuI RGATCY 1 cut(s) 708
PvuII CAGCTG 2 cut(s) 491, 680
RsaI GTAC 3 cut(s) 70, 119, 320
RsaNI GTAC 3 cut(s) 69, 118, 319
RseI CAYNNNNRTG 1 cut(s) 502
SacI GAGCTC 1 cut(s) 870
SapI GCTCTTC 1 cut(s) 282
SaqAI TTAA 4 cut(s) 89, 323, 374, 617
Sau3AI GATC 3 cut(s) 82, 631, 708
Sau96I GGNCC 2 cut(s) 97, 991
SbfI CCTGCAGG 1 cut(s) 1085
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 1 cut(s) 839
SdaI CCTGCAGG 1 cut(s) 1085
SduI GDGCHC 1 cut(s) 870
SfaNI GCATC 8 cut(s) 10, 349, 394, 919, 955, 991, 1060, 1210
SfcI CTRYAG 3 cut(s) 249, 270, 1081
SinI GGWCC 1 cut(s) 991
SmiMI CAYNNNNRTG 1 cut(s) 502
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
SphI GCATGC 1 cut(s) 507
Sse8387I CCTGCAGG 1 cut(s) 1085
Sse9I AATT 6 cut(s) 131, 324, 346, 375, 981, 1168
SseBI AGGCCT 1 cut(s) 1079
SsiI CCGC 4 cut(s) 288, 903, 956, 1235
SspMI CTAG 4 cut(s) 33, 197, 296, 822
SstI GAGCTC 1 cut(s) 870
StuI AGGCCT 1 cut(s) 1079
StyI CCWWGG 2 cut(s) 196, 330
TaaI ACNGT 2 cut(s) 757, 1262
TaqI TCGA 6 cut(s) 339, 917, 1021, 1120, 1194, 1218
TasI AATT 6 cut(s) 131, 324, 346, 375, 981, 1168
TatI WGTACW 2 cut(s) 68, 117
TauI GCSGC 2 cut(s) 905, 959
TfiI GAWTC 1 cut(s) 520
Tru1I TTAA 4 cut(s) 89, 323, 374, 617
Tru9I TTAA 4 cut(s) 89, 323, 374, 617
TscAI CASTG 2 cut(s) 262, 504
TseFI GTSAC 1 cut(s) 877
Tsp45I GTSAC 1 cut(s) 877
TspDTI ATGAA 3 cut(s) 173, 269, 1173
TspGWI ACGGA 1 cut(s) 234
TspRI CASTG 2 cut(s) 262, 504
VpaK11BI GGWCC 1 cut(s) 991
XceI RCATGY 3 cut(s) 69, 507, 1061
XmaJI CCTAGG 1 cut(s) 196
XspI CTAG 4 cut(s) 33, 197, 296, 822
ZrmI AGTACT 1 cut(s) 119
Zsp2I ATGCAT 1 cut(s) 970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.