Rh5AG295200

ankyrin repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002248
Physical Location & Seq
Forward (+)
41025436 .. 41037686
12251 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG295200.1

Sequence Viewer

Length: 477 bp
ATGCAAGGGAGGATGCCTCGAGCTCCTCGGGTACGACTGAGCATGTTCTTGAGGAGCATTTTTCTCTGGAAAACGCAACAGGGTGTCAATGTCAATCCACAAAGTGATGCTGGTTCTCCTTTAATTTGGGCTACTGGTCATGACCAACCAGATGCTGTGAAAGTTCTGTTAGAACACCAGGCTAATCCTAATGCTGAAAGTGATGATGATATTACTCCATTGTTGTCATCTGTGGCAGCTGGTTCACTGGCGTGCTTGGAGTTATTGATTCAGAAGAAATTGAACTCAAAGCTCCCCCTTTACAAAGCTGATACACAAGTTACATGCACCATCTTCAACCCGCATATTGATATCCACCCATTCTTGCTGGCAAAATTCGGTACAACTCAAGCCCCCCCATTTAACCATCGTTCCATATTCATCAATTGGAACTTGCACATTCAAATCAATCTATCTGATTCTCTTCATTGTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 Ank_5 DUF1685 TPR_17 TPR_8

Protein Analysis

158

Amino Acids

17.64

Weight (kDa)

7.82

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 27 - 93 1.1e-07 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 341
AcsI RAATTY 1 cut(s) 374
AdeI CACNNNGTG 1 cut(s) 104
AfaI GTAC 2 cut(s) 33, 382
AgsI TTSAA 3 cut(s) 283, 337, 443
AjnI CCWGG 1 cut(s) 177
AleI CACNNNNGTG 1 cut(s) 250
AluBI AGCT 4 cut(s) 23, 239, 292, 308
AluI AGCT 4 cut(s) 23, 239, 292, 308
Alw21I GWGCWC 1 cut(s) 25
AlwNI CAGNNNCTG 1 cut(s) 155
Ama87I CYCGRG 2 cut(s) 18, 27
ApeKI GCWGC 1 cut(s) 236
ApoI RAATTY 1 cut(s) 374
AvaI CYCGRG 2 cut(s) 18, 27
BaeI ACNNNNGTAYC 2 cut(s) 303, 336
BanII GRGCYC 1 cut(s) 25
Bbv12I GWGCWC 1 cut(s) 25
BbvI GCAGC 1 cut(s) 248
BccI CCATC 2 cut(s) 338, 414
BciT130I CCWGG 1 cut(s) 179
BisI GCNGC 1 cut(s) 237
BlsI GCNGC 1 cut(s) 238
Bme1390I CCNGG 1 cut(s) 179
BmeT110I CYCGRG 2 cut(s) 18, 27
BmrFI CCNGG 1 cut(s) 179
BmsI GCATC 3 cut(s) 3, 97, 142
BplI GAGNNNNNCTC 1 cut(s) 33
BpuEI CTTGAG 2 cut(s) 70, 372
BsaJI CCNNGG 1 cut(s) 26
Bse1I ACTGG 2 cut(s) 139, 252
BseBI CCWGG 1 cut(s) 179
BseDI CCNNGG 1 cut(s) 26
BseGI GGATG 1 cut(s) 18
BseMII CTCAG 1 cut(s) 29
BseNI ACTGG 2 cut(s) 139, 252
BseRI GAGGAG 2 cut(s) 15, 67
BseXI GCAGC 1 cut(s) 248
BsiHKAI GWGCWC 1 cut(s) 25
BsiHKCI CYCGRG 2 cut(s) 18, 27
BsoBI CYCGRG 2 cut(s) 18, 27
Bsp1286I GDGCHC 1 cut(s) 25
BspACI CCGC 1 cut(s) 341
BspCNI CTCAG 1 cut(s) 30
BspHI TCATGA 1 cut(s) 139
BsrI ACTGG 2 cut(s) 139, 252
BssECI CCNNGG 1 cut(s) 26
Bst2UI CCWGG 1 cut(s) 179
Bst6I CTCTTC 1 cut(s) 468
BstC8I GCNNGC 2 cut(s) 253, 369
BstDEI CTNAG 1 cut(s) 38
BstF5I GGATG 1 cut(s) 18
BstNI CCWGG 1 cut(s) 179
BstNSI RCATGY 2 cut(s) 46, 327
BstSCI CCNGG 1 cut(s) 177
BstV1I GCAGC 1 cut(s) 248
BtsCI GGATG 1 cut(s) 18
BtsIMutI CAGTG 1 cut(s) 245
Cac8I GCNNGC 2 cut(s) 253, 369
CaiI CAGNNNCTG 1 cut(s) 155
CciI TCATGA 1 cut(s) 139
Csp6I GTAC 2 cut(s) 32, 381
CviAII CATG 3 cut(s) 43, 140, 324
CviJI RGCY 7 cut(s) 23, 131, 182, 239, 292, 308, 392
CviKI_1 RGCY 7 cut(s) 23, 131, 182, 239, 292, 308, 392
CviQI GTAC 2 cut(s) 32, 381
DdeI CTNAG 1 cut(s) 38
DraIII CACNNNGTG 1 cut(s) 104
Eam1104I CTCTTC 1 cut(s) 468
EarI CTCTTC 1 cut(s) 468
Ecl136II GAGCTC 1 cut(s) 23
Eco24I GRGCYC 1 cut(s) 25
Eco32I GATATC 1 cut(s) 352
Eco53kI GAGCTC 1 cut(s) 23
Eco88I CYCGRG 2 cut(s) 18, 27
EcoICRI GAGCTC 1 cut(s) 23
EcoRII CCWGG 1 cut(s) 177
EcoRV GATATC 1 cut(s) 352
EcoT38I GRGCYC 1 cut(s) 25
FaeI CATG 3 cut(s) 46, 143, 327
FaiI YATR 5 cut(s) 44, 141, 325, 345, 416
FatI CATG 3 cut(s) 42, 139, 323
FauI CCCGC 1 cut(s) 348
Fnu4HI GCNGC 1 cut(s) 237
FokI GGATG 1 cut(s) 25
FriOI GRGCYC 1 cut(s) 25
Fsp4HI GCNGC 1 cut(s) 237
GluI GCNGC 1 cut(s) 237
Hin1II CATG 3 cut(s) 46, 143, 327
HinfI GANTC 2 cut(s) 268, 458
Hpy166II GTNNAC 1 cut(s) 245
Hpy188I TCNGA 2 cut(s) 273, 457
Hpy188III TCNNGA 3 cut(s) 49, 67, 140
Hpy8I GTNNAC 1 cut(s) 245
HpyCH4V TGCA 3 cut(s) 4, 327, 436
HpyF3I CTNAG 1 cut(s) 38
Hsp92II CATG 3 cut(s) 46, 143, 327
LmnI GCTCC 3 cut(s) 28, 54, 297
Lsp1109I GCAGC 1 cut(s) 248
LweI GCATC 3 cut(s) 3, 97, 142
MaeIII GTNAC 1 cut(s) 319
MboII GAAGA 3 cut(s) 286, 325, 455
MfeI CAATTG 1 cut(s) 424
MhlI GDGCHC 1 cut(s) 25
MluCI AATT 4 cut(s) 123, 278, 374, 424
MnlI CCTC 4 cut(s) 3, 27, 36, 45
MseI TTAA 2 cut(s) 122, 402
MslI CAYNNNNRTG 1 cut(s) 250
MspA1I CMGCKG 1 cut(s) 239
MspR9I CCNGG 1 cut(s) 179
MunI CAATTG 1 cut(s) 424
MvaI CCWGG 1 cut(s) 179
NlaIII CATG 3 cut(s) 46, 143, 327
NspI RCATGY 2 cut(s) 46, 327
OliI CACNNNNGTG 1 cut(s) 250
PaeR7I CTCGAG 1 cut(s) 18
PagI TCATGA 1 cut(s) 139
PfeI GAWTC 2 cut(s) 268, 458
PkrI GCNGC 1 cut(s) 238
Psp124BI GAGCTC 1 cut(s) 25
Psp6I CCWGG 1 cut(s) 177
PspGI CCWGG 1 cut(s) 177
PspXI VCTCGAGB 1 cut(s) 18
PstNI CAGNNNCTG 1 cut(s) 155
PvuII CAGCTG 1 cut(s) 239
RsaI GTAC 2 cut(s) 33, 382
RsaNI GTAC 2 cut(s) 32, 381
RseI CAYNNNNRTG 1 cut(s) 250
SacI GAGCTC 1 cut(s) 25
SaqAI TTAA 2 cut(s) 122, 402
SatI GCNGC 1 cut(s) 237
ScrFI CCNGG 1 cut(s) 179
SduI GDGCHC 1 cut(s) 25
SetI ASST 4 cut(s) 25, 241, 294, 310
SfaNI GCATC 3 cut(s) 3, 97, 142
Sfr274I CTCGAG 1 cut(s) 18
SlaI CTCGAG 1 cut(s) 18
SmiMI CAYNNNNRTG 1 cut(s) 250
SmlI CTYRAG 3 cut(s) 18, 49, 387
SmoI CTYRAG 3 cut(s) 18, 49, 387
Sse9I AATT 4 cut(s) 123, 278, 374, 424
SsiI CCGC 1 cut(s) 341
SstI GAGCTC 1 cut(s) 25
StyD4I CCNGG 1 cut(s) 177
TaqI TCGA 1 cut(s) 19
TasI AATT 4 cut(s) 123, 278, 374, 424
TfiI GAWTC 2 cut(s) 268, 458
Tru1I TTAA 2 cut(s) 122, 402
Tru9I TTAA 2 cut(s) 122, 402
TscAI CASTG 1 cut(s) 252
TseI GCWGC 1 cut(s) 236
TspDTI ATGAA 2 cut(s) 409, 455
TspRI CASTG 1 cut(s) 252
XapI RAATTY 1 cut(s) 374
XceI RCATGY 2 cut(s) 46, 327
XhoI CTCGAG 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.