RchiOBHm_Chr3g0450651

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002248
Physical Location & Seq
Forward (+)
2107580 .. 2112465
4886 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41797

Sequence Viewer

Length: 1389 bp
ATGGCCCCTGACGCTTCTGATGCACTTGCAGCTAGGGAAATAGTGCAGCAGTTTCTGAATGCTGCATGTACTGGGGATGTTGATCTCTTAAAGAAGTTGGCGGGGAAACTTGATGAAGGGAAGGGATTGGCCAAAACTGTCGATGATATTAAGGATGCCAACAAGCGCGGCGCGCTTCATTTTGCTGCAAGAGAGGGCCAGTCTGAGATGTGCAAGTACTTGCTGGAGGAATTGAAACTTGATGTTGATACCAAAGACGAAGATGGCGACACTCCCCTTATTCATGCTGCTCGCCTAGGCCATACTGATACTGCAAAACTGCTTCTTGAGTATGGTGCCAATCCGTCTATAGCCAGTGATTTAGGGGCTACAGCTCTTCATCATTCCGCCGGACTAGGAAACATTGAGTTGCTGAGGTACTTAATTTCCAAGGGTGTTGATGTCAATTCACAAAGTGATGCTGGTTCTCCTTTAATTTGGGCTGCTGGTCATGACCAACCAGATGCTGTGAAAGTTCTGTTAGAACACCACGCTAATCCTAATACTGAAAGTGATGATGATATTACTCCATTGTTGTCATCTGTGGCAGCTGGTTCACTGGCATGCTTGGAGTTATTGATTCAGGCAGGTGCCAAAGTAAATATGAGTGCTGGTGGAGCGACTCCTTTACACATTGCTGCTGATATTGGGAACTTGGAGATTATAAAATGTTTGTTAAAAGCTGGAGCTGATCCCAATGTAATTGATGAGGAGGGTTTGAAGCCAATACAGGTTGCAGCTGCAAGTGGGAACCGAAGCGCTGTGGAGATCCTCTTTCCCTTGACATCACAAGTTGAAACCATTTCAAAATGGACAGTTGACGGCATACTTGAGTATATGCAGTCTGAAACAAGCAGACAACAGGAACAACCGCGAAATACTAAGGAACTTAATGTCCCAAAAGACTCTACTTCACTGAAGAAAGAGCTCCCTGAGGTGACACCTGAGGCAAAGAAAAGAGCCGCAGAAGCAAAATTGAGAGGCGGTGATGCTTTCAGTAGAAAGGATTATCAAGCGGCTGTTGATGCATACACGCAGGCAATTGATTTGGACCCAACTGATGCCATTTTTCTTTCCAATCGAAGCCTTTGTTGGCTTCGTCTTGGCCAAGCTGAACATGCCTTAGCTGATGCAAAGGCCTGCAGGGAATTGAAACCGGATTGGCCAAAGGCTTGCTTTCGAGAAGGTGCAGCATTGCGCCTAATGCAGAAGTTTGATGAAGCAGCCAATTGTTTTTATGAGGGCGTGCAGCTCGACCCTGAAAGTAAGGAACTCGTTGATGCTTTCAGGGAAGCGGTTGAAGCTGGAAGGCAGTTCCACAGTAAAGATAAACAAAAACCAAAACCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 Ank_5 DUF1685 TPR_17 TPR_8

Protein Analysis

462

Amino Acids

49.83

Weight (kDa)

5.06

Isoelectric Point (pI)

32.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 20 - 116 9.6e-17 Ankyrin repeats (3 copies)
Ank_4 PF13637 59 - 101 2e-06 Ankyrin repeats (many copies)
Ank PF00023 88 - 118 2.3e-07 Ankyrin repeat
Ank_4 PF13637 95 - 142 1.1e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 119 - 182 1.1e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 158 - 249 1.6e-16 Ankyrin repeats (3 copies)
Ank_2 PF12796 195 - 273 1.4e-12 Ankyrin repeats (3 copies)
Ank_4 PF13637 197 - 239 6.4e-07 Ankyrin repeats (many copies)
Ank_5 PF13857 206 - 259 1.1e-07 Ankyrin repeats (many copies)
Ank PF00023 219 - 249 7e-09 Ankyrin repeat
Ank_3 PF13606 219 - 247 3.8e-06 Ankyrin repeat
TPR_BSK1_C PF25575 331 - 406 7e-13 Serine/threonine-protein kinase BSK1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 704
AarI CACCTGC 1 cut(s) 617
Acc36I ACCTGC 1 cut(s) 617
AccB1I GGYRCC 2 cut(s) 335, 629
AccII CGCG 3 cut(s) 168, 173, 913
AciI CCGC 8 cut(s) 101, 168, 387, 911, 1002, 1023, 1055, 1334
AclWI GGATC 2 cut(s) 725, 802
AcoI YGGCCR 3 cut(s) 129, 1144, 1202
AcuI CTGAAG 1 cut(s) 977
AdeI CACNNNGTG 1 cut(s) 455
AfaI GTAC 3 cut(s) 70, 218, 419
AfeI AGCGCT 1 cut(s) 799
AgsI TTSAA 6 cut(s) 235, 760, 836, 846, 1192, 1340
AjuI GAANNNNNNNTTGG 2 cut(s) 1114, 1146
AloI GAACNNNNNNTCC 2 cut(s) 918, 950
Alw21I GWGCWC 1 cut(s) 969
AlwI GGATC 2 cut(s) 725, 802
AlwNI CAGNNNCTG 2 cut(s) 55, 506
Aor51HI AGCGCT 1 cut(s) 799
AoxI GGCC 7 cut(s) 3, 129, 196, 298, 1144, 1176, 1202
AspA2I CCTAGG 1 cut(s) 295
AspLEI GCGC 5 cut(s) 168, 173, 175, 800, 1239
AspS9I GGNCC 3 cut(s) 4, 196, 1090
AsuHPI GGTGA 2 cut(s) 988, 1037
AvaII GGWCC 1 cut(s) 1090
AvrII CCTAGG 1 cut(s) 295
AxyI CCTNAGG 2 cut(s) 972, 984
BalI TGGCCA 3 cut(s) 131, 1146, 1204
BanI GGYRCC 2 cut(s) 335, 629
BanII GRGCYC 1 cut(s) 969
Bbv12I GWGCWC 1 cut(s) 969
BbvCI CCTCAGC 1 cut(s) 413
BccI CCATC 1 cut(s) 257
BceAI ACGGC 1 cut(s) 877
BfaI CTAG 3 cut(s) 33, 296, 395
BfmI CTRYAG 3 cut(s) 348, 369, 1180
BfoI RGCGCY 1 cut(s) 801
BfuAI ACCTGC 1 cut(s) 617
BlnI CCTAGG 1 cut(s) 295
BmcAI AGTACT 1 cut(s) 218
Bme18I GGWCC 1 cut(s) 1090
BmgT120I GGNCC 3 cut(s) 4, 196, 1090
BmiI GGNNCC 5 cut(s) 6, 337, 631, 791, 1092
BmrI ACTGGG 1 cut(s) 81
BmsI GCATC 9 cut(s) 10, 145, 448, 493, 1018, 1054, 1090, 1159, 1309
BmuI ACTGGG 1 cut(s) 81
BpmI CTGGAG 2 cut(s) 245, 744
Bpu10I CCTNAGC 2 cut(s) 413, 1162
BpuEI CTTGAG 2 cut(s) 347, 890
BsaBI GATNNNNATC 1 cut(s) 81
BsaJI CCNNGG 2 cut(s) 295, 429
BsaWI WCCGGW 1 cut(s) 1195
Bse1I ACTGG 4 cut(s) 76, 199, 354, 603
Bse21I CCTNAGG 2 cut(s) 972, 984
Bse3DI GCAATG 2 cut(s) 672, 1232
Bse8I GATNNNNATC 1 cut(s) 81
BseDI CCNNGG 2 cut(s) 295, 429
BseGI GGATG 2 cut(s) 82, 160
BseJI GATNNNNATC 1 cut(s) 81
BseMI GCAATG 2 cut(s) 672, 1232
BseMII CTCAG 4 cut(s) 195, 404, 963, 975
BseNI ACTGG 4 cut(s) 76, 199, 354, 603
BsePI GCGCGC 1 cut(s) 171
BseRI GAGGAG 1 cut(s) 764
BsgI GTGCAG 3 cut(s) 65, 1248, 1307
Bsh1236I CGCG 3 cut(s) 168, 173, 913
BshFI GGCC 7 cut(s) 5, 131, 198, 300, 1146, 1178, 1204
BshNI GGYRCC 2 cut(s) 335, 629
BsiHKAI GWGCWC 1 cut(s) 969
BsiSI CCGG 2 cut(s) 390, 1196
BslFI GGGAC 1 cut(s) 920
BsmFI GGGAC 1 cut(s) 920
BsmI GAATGC 1 cut(s) 64
BsnI GGCC 7 cut(s) 5, 131, 198, 300, 1146, 1178, 1204
Bsp1286I GDGCHC 1 cut(s) 969
Bsp143I GATC 3 cut(s) 82, 730, 807
BspACI CCGC 8 cut(s) 101, 168, 387, 911, 1002, 1023, 1055, 1334
BspANI GGCC 7 cut(s) 5, 131, 198, 300, 1146, 1178, 1204
BspCNI CTCAG 4 cut(s) 196, 405, 964, 976
BspFNI CGCG 3 cut(s) 168, 173, 913
BspHI TCATGA 1 cut(s) 490
BspLI GGNNCC 5 cut(s) 6, 337, 631, 791, 1092
BspMAI CTGCAG 1 cut(s) 1184
BspMI ACCTGC 1 cut(s) 617
BspPI GGATC 2 cut(s) 725, 802
BspQI GCTCTTC 1 cut(s) 381
BspT107I GGYRCC 2 cut(s) 335, 629
BsrDI GCAATG 2 cut(s) 672, 1232
BsrI ACTGG 4 cut(s) 76, 199, 354, 603
BssECI CCNNGG 2 cut(s) 295, 429
BssHII GCGCGC 1 cut(s) 171
BssMI GATC 3 cut(s) 82, 730, 807
BssT1I CCWWGG 2 cut(s) 295, 429
Bst4CI ACNGT 3 cut(s) 139, 856, 1361
Bst6I CTCTTC 1 cut(s) 381
BstC8I GCNNGC 7 cut(s) 173, 292, 604, 1077, 1180, 1213, 1286
BstDEI CTNAG 7 cut(s) 204, 413, 921, 972, 984, 1162, 1386
BstF5I GGATG 2 cut(s) 82, 160
BstFNI CGCG 3 cut(s) 168, 173, 913
BstH2I RGCGCY 1 cut(s) 801
BstHHI GCGC 5 cut(s) 168, 173, 175, 800, 1239
BstKTI GATC 3 cut(s) 85, 733, 810
BstMBI GATC 3 cut(s) 82, 730, 807
BstNSI RCATGY 3 cut(s) 69, 606, 1160
BstSFI CTRYAG 3 cut(s) 348, 369, 1180
BstUI CGCG 3 cut(s) 168, 173, 913
BstX2I RGATCY 1 cut(s) 807
BstYI RGATCY 1 cut(s) 807
Bsu36I CCTNAGG 2 cut(s) 972, 984
BsuRI GGCC 7 cut(s) 5, 131, 198, 300, 1146, 1178, 1204
BtsCI GGATG 2 cut(s) 82, 160
BtsIMutI CAGTG 3 cut(s) 361, 596, 953
BveI ACCTGC 1 cut(s) 617
Cac8I GCNNGC 7 cut(s) 173, 292, 604, 1077, 1180, 1213, 1286
CaiI CAGNNNCTG 2 cut(s) 55, 506
CciI TCATGA 1 cut(s) 490
CfoI GCGC 5 cut(s) 168, 173, 175, 800, 1239
Cfr13I GGNCC 3 cut(s) 4, 196, 1090
CseI GACGC 1 cut(s) 20
Csp6I GTAC 3 cut(s) 69, 217, 418
CviAII CATG 5 cut(s) 66, 284, 491, 603, 1157
CviQI GTAC 3 cut(s) 69, 217, 418
DdeI CTNAG 7 cut(s) 204, 413, 921, 972, 984, 1162, 1386
DpnI GATC 3 cut(s) 84, 732, 809
DpnII GATC 3 cut(s) 82, 730, 807
DraIII CACNNNGTG 1 cut(s) 455
EaeI YGGCCR 3 cut(s) 129, 1144, 1202
Eam1104I CTCTTC 1 cut(s) 381
EarI CTCTTC 1 cut(s) 381
EciI GGCGGA 1 cut(s) 376
Ecl136II GAGCTC 1 cut(s) 967
Eco130I CCWWGG 2 cut(s) 295, 429
Eco147I AGGCCT 1 cut(s) 1178
Eco24I GRGCYC 1 cut(s) 969
Eco47I GGWCC 1 cut(s) 1090
Eco47III AGCGCT 1 cut(s) 799
Eco53kI GAGCTC 1 cut(s) 967
Eco57I CTGAAG 1 cut(s) 977
Eco81I CCTNAGG 2 cut(s) 972, 984
EcoICRI GAGCTC 1 cut(s) 967
EcoT14I CCWWGG 2 cut(s) 295, 429
EcoT22I ATGCAT 1 cut(s) 1069
EcoT38I GRGCYC 1 cut(s) 969
ErhI CCWWGG 2 cut(s) 295, 429
FaeI CATG 5 cut(s) 69, 287, 494, 606, 1160
FalI AAGNNNNNCTT 2 cut(s) 1199, 1231
FaqI GGGAC 1 cut(s) 920
FatI CATG 5 cut(s) 65, 283, 490, 602, 1156
FauI CCCGC 1 cut(s) 94
FokI GGATG 2 cut(s) 89, 167
FriOI GRGCYC 1 cut(s) 969
FspBI CTAG 3 cut(s) 33, 296, 395
GlaI GCGC 5 cut(s) 167, 172, 174, 799, 1238
GsuI CTGGAG 2 cut(s) 245, 744
HaeII RGCGCY 1 cut(s) 801
HaeIII GGCC 7 cut(s) 5, 131, 198, 300, 1146, 1178, 1204
HapII CCGG 2 cut(s) 390, 1196
HgaI GACGC 1 cut(s) 20
HhaI GCGC 5 cut(s) 168, 173, 175, 800, 1239
Hin1II CATG 5 cut(s) 69, 287, 494, 606, 1160
Hin6I GCGC 5 cut(s) 166, 171, 173, 798, 1237
HinP1I GCGC 5 cut(s) 166, 171, 173, 798, 1237
HincII GTYRAC 1 cut(s) 859
HindII GTYRAC 1 cut(s) 859
HinfI GANTC 3 cut(s) 619, 661, 944
HpaII CCGG 2 cut(s) 390, 1196
HphI GGTGA 2 cut(s) 988, 1037
Hpy166II GTNNAC 2 cut(s) 596, 859
Hpy188I TCNGA 4 cut(s) 19, 57, 205, 886
Hpy188III TCNNGA 3 cut(s) 326, 491, 1220
Hpy8I GTNNAC 2 cut(s) 596, 859
HpyAV CCTTC 4 cut(s) 110, 115, 1217, 1341
HpyCH4III ACNGT 3 cut(s) 139, 856, 1361
HpyF3I CTNAG 7 cut(s) 204, 413, 921, 972, 984, 1162, 1386
Hsp92II CATG 5 cut(s) 69, 287, 494, 606, 1160
HspAI GCGC 5 cut(s) 166, 171, 173, 798, 1237
Kzo9I GATC 3 cut(s) 82, 730, 807
LguI GCTCTTC 1 cut(s) 381
LmnI GCTCC 3 cut(s) 656, 725, 972
LweI GCATC 9 cut(s) 10, 145, 448, 493, 1018, 1054, 1090, 1159, 1309
MaeI CTAG 3 cut(s) 33, 296, 395
MaeIII GTNAC 1 cut(s) 976
MalI GATC 3 cut(s) 84, 732, 809
MboI GATC 3 cut(s) 82, 730, 807
MboII GAAGA 3 cut(s) 272, 368, 970
MfeI CAATTG 2 cut(s) 1080, 1267
MflI RGATCY 1 cut(s) 807
MhlI GDGCHC 1 cut(s) 969
MlsI TGGCCA 3 cut(s) 131, 1146, 1204
MluCI AATT 9 cut(s) 230, 423, 445, 474, 741, 1013, 1080, 1187, 1267
MluNI TGGCCA 3 cut(s) 131, 1146, 1204
MlyI GAGTC 2 cut(s) 655, 938
Mox20I TGGCCA 3 cut(s) 131, 1146, 1204
Mph1103I ATGCAT 1 cut(s) 1069
MscI TGGCCA 3 cut(s) 131, 1146, 1204
MseI TTAA 6 cut(s) 89, 150, 422, 473, 716, 930
MslI CAYNNNNRTG 1 cut(s) 601
Msp20I TGGCCA 3 cut(s) 131, 1146, 1204
MspA1I CMGCKG 2 cut(s) 590, 779
MspI CCGG 2 cut(s) 390, 1196
MteI GCGCNGCGC 1 cut(s) 169
MunI CAATTG 2 cut(s) 1080, 1267
Mva1269I GAATGC 1 cut(s) 64
MvnI CGCG 3 cut(s) 168, 173, 913
NdeII GATC 3 cut(s) 82, 730, 807
NlaIII CATG 5 cut(s) 69, 287, 494, 606, 1160
NlaIV GGNNCC 5 cut(s) 6, 337, 631, 791, 1092
NmuCI GTSAC 1 cut(s) 976
NsiI ATGCAT 1 cut(s) 1069
NspI RCATGY 3 cut(s) 69, 606, 1160
PaeI GCATGC 1 cut(s) 606
PagI TCATGA 1 cut(s) 490
PaqCI CACCTGC 1 cut(s) 617
PauI GCGCGC 1 cut(s) 171
PceI AGGCCT 1 cut(s) 1178
PciSI GCTCTTC 1 cut(s) 381
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PctI GAATGC 1 cut(s) 64
PfeI GAWTC 1 cut(s) 619
PleI GAGTC 2 cut(s) 655, 938
PpsI GAGTC 2 cut(s) 655, 938
PsiI TTATAA 1 cut(s) 704
Psp124BI GAGCTC 1 cut(s) 969
PspN4I GGNNCC 5 cut(s) 6, 337, 631, 791, 1092
PspPI GGNCC 3 cut(s) 4, 196, 1090
PstI CTGCAG 1 cut(s) 1184
PstNI CAGNNNCTG 2 cut(s) 55, 506
PsuI RGATCY 1 cut(s) 807
PteI GCGCGC 1 cut(s) 171
PvuII CAGCTG 2 cut(s) 590, 779
RsaI GTAC 3 cut(s) 70, 218, 419
RsaNI GTAC 3 cut(s) 69, 217, 418
RseI CAYNNNNRTG 1 cut(s) 601
SacI GAGCTC 1 cut(s) 969
SapI GCTCTTC 1 cut(s) 381
SaqAI TTAA 6 cut(s) 89, 150, 422, 473, 716, 930
Sau3AI GATC 3 cut(s) 82, 730, 807
Sau96I GGNCC 3 cut(s) 4, 196, 1090
SbfI CCTGCAGG 1 cut(s) 1184
ScaI AGTACT 1 cut(s) 218
SchI GAGTC 2 cut(s) 655, 938
SdaI CCTGCAGG 1 cut(s) 1184
SduI GDGCHC 1 cut(s) 969
SfaNI GCATC 9 cut(s) 10, 145, 448, 493, 1018, 1054, 1090, 1159, 1309
SfcI CTRYAG 3 cut(s) 348, 369, 1180
SinI GGWCC 1 cut(s) 1090
SmiMI CAYNNNNRTG 1 cut(s) 601
SmlI CTYRAG 2 cut(s) 326, 869
SmoI CTYRAG 2 cut(s) 326, 869
SphI GCATGC 1 cut(s) 606
Sse8387I CCTGCAGG 1 cut(s) 1184
Sse9I AATT 9 cut(s) 230, 423, 445, 474, 741, 1013, 1080, 1187, 1267
SseBI AGGCCT 1 cut(s) 1178
SsiI CCGC 8 cut(s) 101, 168, 387, 911, 1002, 1023, 1055, 1334
SspMI CTAG 3 cut(s) 33, 296, 395
SstI GAGCTC 1 cut(s) 969
StuI AGGCCT 1 cut(s) 1178
StyI CCWWGG 2 cut(s) 295, 429
TaaI ACNGT 3 cut(s) 139, 856, 1361
TaqI TCGA 4 cut(s) 141, 1120, 1219, 1293
TasI AATT 9 cut(s) 230, 423, 445, 474, 741, 1013, 1080, 1187, 1267
TatI WGTACW 2 cut(s) 68, 216
TauI GCSGC 3 cut(s) 171, 1004, 1058
TfiI GAWTC 1 cut(s) 619
Tru1I TTAA 6 cut(s) 89, 150, 422, 473, 716, 930
Tru9I TTAA 6 cut(s) 89, 150, 422, 473, 716, 930
TscAI CASTG 3 cut(s) 361, 603, 960
TseFI GTSAC 1 cut(s) 976
Tsp45I GTSAC 1 cut(s) 976
TspDTI ATGAA 5 cut(s) 129, 167, 272, 368, 1272
TspGWI ACGGA 1 cut(s) 333
TspRI CASTG 3 cut(s) 361, 603, 960
VpaK11BI GGWCC 1 cut(s) 1090
XceI RCATGY 3 cut(s) 69, 606, 1160
XmaJI CCTAGG 1 cut(s) 295
XspI CTAG 3 cut(s) 33, 296, 395
ZrmI AGTACT 1 cut(s) 218
Zsp2I ATGCAT 1 cut(s) 1069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.