MD04G1226000.v1.1

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002248
Physical Location & Seq
Reverse (-)
30563472 .. 30567399
3928 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1226000.v1.1.491

Sequence Viewer

Length: 1383 bp
ATGGCCGCTGACGCTTCTGCTGCCCTTGCAGTGAGGGATAAAGTTCAACAATTTCTCAATGCTGCTGTTACTGGGAATCTTGATCTCTTAAAGAATTTGGCCGGGCAGCTTGATGAAGGGAAGGATTTGGCCAAAACTGTGGCTGATATTAAGGATGCCAACAAGCGAGGGGCCCTTCATTTTGCTGCGAGAGAGGGAAAAACTGAGGTGTGCAAGTACTTGCTGGAGGAACTGAAACTTGACGTTGATACAAAGGATGAAGACGGTGAAACTGCTCTTATCCATGCTGCTCGGCAAGGACATACTGAAACTGCAAAATACCTTCTTGATTCTGGTGCCAATCCTGGGATAGCTAGTGAATTGGGAGCCACAGCTCTTCATCATTCTGCTGGATTAGGAGACATTGAGTTGTTGAGGTATTTGATTTCCAAAGGTGCTGATGTTAATTCACAAAGCGACGCTGGAAGTCCTTTAATTTGGGCTGCTGGTCATGGTCAACAAGATGCTGTGAAAGTTCTATTAGAACACAATGCTAATCCAAATGCTGAAAATGATGACAATATTACGCCCTTGCTGTCATCTGTTGCTGCTGGTTCACTGCCATGCTTAGAATTATTGATTCAGGCTGGTGCCAAAGTAAATATTAGTGCTGGTGGAGCATCCCCTTTGCACATTGCTGCCGATATTGGGAGCCCAGAGATTTTGCAATTATTGTTAAAAGGTGGAGGTGATCCCAATATCACTGATGAGGATGGCTTGAAGCCAATACAGGTTGCAGCTGGAAGAGGGAACCGCAGTGCCGTTGAGATTCTCTTTCCCTTGACATCAAAAGTTGAAAGTATCCCAAAATGGACAGTTGACGACATACTTGAGTATATGCAGTCTGAAACAAGTAGACAACAGGAAGCAGTGGGAAATTTTAAGGAACTTAATGTGTCGAAAGATTGTGCGTTACCGAAGAAAGATCTGCCTGAGGTGTCACCTGAAGCGAAGAAGAAAGCTGCAGAAGCAAAATCAAGAGGAACTGATGCTTTCAAAAGAAAGGATTATCAGACAGCCATTGATGCGTATACACAGGCTATTGATCTGGACCCAACCGATGCTACTTTGTTTTCTAACCGAAGCCTTTGTTGGATGTGTCTTGGTCAAGCCGAGCATGCATTAGCTGACGGAAAGACCTCCCGGGAATTAAAGCCAGAATGGGGGAAGGCTTGGTACCGAGAAGGTGCAGCGTTGCGACTGTTGGAGAAGTTTGATGAAGCTGCCAATTCTTTCTATGAGGGCGTGAGGCGGGACCCCGAAAACAAGGCACTTGTAGATGCTTTCAGAGAAGCGGTTGAAGCTGGACGGAAATTTCATGGCGCTGATAAACAGAAACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 Ank_5 DUF1685 TPR_17 TPR_8

Protein Analysis

461

Amino Acids

49.33

Weight (kDa)

5.16

Isoelectric Point (pI)

33.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 20 - 116 5.5e-17 Ankyrin repeats (3 copies)
Ank_4 PF13637 59 - 109 2.8e-12 Ankyrin repeats (many copies)
Ank_5 PF13857 79 - 127 9.7e-06 Ankyrin repeats (many copies)
Ank PF00023 88 - 117 1.8e-06 Ankyrin repeat
Ank_4 PF13637 95 - 142 1.7e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 113 - 183 7.5e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 158 - 207 1.3e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 178 - 249 5.7e-10 Ankyrin repeats (3 copies)
Ank_5 PF13857 206 - 259 5.3e-08 Ankyrin repeats (many copies)
Ank PF00023 219 - 249 1.7e-06 Ankyrin repeat
Ank_2 PF12796 223 - 273 1.5e-06 Ankyrin repeats (3 copies)
TPR_BSK1_C PF25575 332 - 406 4.5e-15 Serine/threonine-protein kinase BSK1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1215
AccB1I GGYRCC 3 cut(s) 335, 629, 1215
AccI GTMKAC 2 cut(s) 895, 1070
AciI CCGC 4 cut(s) 6, 793, 1291, 1334
AclWI GGATC 1 cut(s) 725
AcoI YGGCCR 3 cut(s) 3, 99, 129
AcsI RAATTY 3 cut(s) 94, 916, 1352
AcuI CTGAAG 1 cut(s) 1005
AfaI GTAC 2 cut(s) 218, 1217
AfiI CCNNNNNNNGG 3 cut(s) 345, 687, 1202
AgsI TTSAA 5 cut(s) 47, 760, 836, 1036, 1340
AjnI CCWGG 1 cut(s) 343
AjuI GAANNNNNNNTTGG 2 cut(s) 1114, 1146
AluBI AGCT 8 cut(s) 109, 353, 374, 779, 1001, 1166, 1262, 1343
AluI AGCT 8 cut(s) 109, 353, 374, 779, 1001, 1166, 1262, 1343
Alw26I GTCTC 1 cut(s) 393
AlwI GGATC 1 cut(s) 725
Ama87I CYCGRG 1 cut(s) 1182
AoxI GGCC 4 cut(s) 3, 99, 129, 171
ApaI GGGCCC 1 cut(s) 175
ApoI RAATTY 3 cut(s) 94, 916, 1352
Asp718I GGTACC 1 cut(s) 1215
AspLEI GCGC 1 cut(s) 1364
AspS9I GGNCC 4 cut(s) 171, 172, 1090, 1294
AsuC2I CCSGG 3 cut(s) 103, 1183, 1184
AsuHPI GGTGA 3 cut(s) 278, 740, 972
AvaI CYCGRG 1 cut(s) 1182
AvaII GGWCC 2 cut(s) 1090, 1294
AxyI CCTNAGG 1 cut(s) 972
BaeGI GKGCMC 1 cut(s) 175
BalI TGGCCA 1 cut(s) 131
BanI GGYRCC 3 cut(s) 335, 629, 1215
BanII GRGCYC 2 cut(s) 175, 695
BarI GAAGNNNNNNTAC 2 cut(s) 1199, 1231
BbsI GAAGAC 1 cut(s) 267
BccI CCATC 1 cut(s) 746
BceAI ACGGC 1 cut(s) 785
BciT130I CCWGG 1 cut(s) 345
BciVI GTATCC 1 cut(s) 851
BcnI CCSGG 3 cut(s) 103, 1183, 1184
BcoDI GTCTC 1 cut(s) 393
BfaI CTAG 1 cut(s) 354
BfmI CTRYAG 1 cut(s) 1002
BfoI RGCGCY 1 cut(s) 1365
BfuI GTATCC 1 cut(s) 851
BglII AGATCT 1 cut(s) 964
BmcAI AGTACT 1 cut(s) 218
Bme1390I CCNGG 4 cut(s) 103, 345, 1183, 1184
Bme18I GGWCC 2 cut(s) 1090, 1294
BmeT110I CYCGRG 1 cut(s) 1182
BmgT120I GGNCC 4 cut(s) 171, 172, 1090, 1294
BmrFI CCNGG 4 cut(s) 103, 345, 1183, 1184
BmrI ACTGGG 1 cut(s) 81
BmsI GCATC 7 cut(s) 145, 493, 668, 1018, 1054, 1090, 1309
BmuI ACTGGG 1 cut(s) 81
BpiI GAAGAC 1 cut(s) 267
BpmI CTGGAG 1 cut(s) 245
BpuEI CTTGAG 1 cut(s) 890
BpuMI CCSGG 3 cut(s) 103, 1183, 1184
BsaJI CCNNGG 2 cut(s) 344, 1182
Bsc4I CCNNNNNNNGG 3 cut(s) 345, 687, 1202
Bse1I ACTGG 1 cut(s) 76
Bse21I CCTNAGG 1 cut(s) 972
Bse3DI GCAATG 1 cut(s) 672
BseBI CCWGG 1 cut(s) 345
BseDI CCNNGG 2 cut(s) 344, 1182
BseGI GGATG 5 cut(s) 160, 262, 659, 757, 1140
BseLI CCNNNNNNNGG 3 cut(s) 345, 687, 1202
BseMI GCAATG 1 cut(s) 672
BseMII CTCAG 2 cut(s) 195, 963
BseNI ACTGG 1 cut(s) 76
BseSI GKGCMC 1 cut(s) 175
BsgI GTGCAG 1 cut(s) 1248
BshFI GGCC 4 cut(s) 5, 101, 131, 173
BshNI GGYRCC 3 cut(s) 335, 629, 1215
BsiHKCI CYCGRG 1 cut(s) 1182
BsiSI CCGG 2 cut(s) 102, 1183
BslFI GGGAC 1 cut(s) 1307
BslI CCNNNNNNNGG 3 cut(s) 345, 687, 1202
BsmAI GTCTC 1 cut(s) 393
BsmFI GGGAC 1 cut(s) 1307
BsnI GGCC 4 cut(s) 5, 101, 131, 173
BsoBI CYCGRG 1 cut(s) 1182
Bsp120I GGGCCC 1 cut(s) 171
Bsp1286I GDGCHC 2 cut(s) 175, 695
Bsp143I GATC 4 cut(s) 82, 730, 964, 1084
BspACI CCGC 4 cut(s) 6, 793, 1291, 1334
BspANI GGCC 4 cut(s) 5, 101, 131, 173
BspCNI CTCAG 2 cut(s) 196, 964
BspMAI CTGCAG 1 cut(s) 1006
BspPI GGATC 1 cut(s) 725
BspQI GCTCTTC 1 cut(s) 381
BspT107I GGYRCC 3 cut(s) 335, 629, 1215
BsrDI GCAATG 1 cut(s) 672
BsrI ACTGG 1 cut(s) 76
BssECI CCNNGG 2 cut(s) 344, 1182
BssMI GATC 4 cut(s) 82, 730, 964, 1084
BssNAI GTATAC 1 cut(s) 1071
Bst1107I GTATAC 1 cut(s) 1071
Bst2UI CCWGG 1 cut(s) 345
Bst4CI ACNGT 4 cut(s) 139, 266, 856, 1242
Bst6I CTCTTC 2 cut(s) 381, 778
BstC8I GCNNGC 1 cut(s) 1158
BstDEI CTNAG 3 cut(s) 204, 607, 972
BstF5I GGATG 5 cut(s) 160, 262, 659, 757, 1140
BstH2I RGCGCY 1 cut(s) 1365
BstHHI GCGC 1 cut(s) 1364
BstKTI GATC 4 cut(s) 85, 733, 967, 1087
BstMAI GTCTC 1 cut(s) 393
BstMBI GATC 4 cut(s) 82, 730, 964, 1084
BstMWI GCNNNNNNNGC 8 cut(s) 11, 20, 26, 656, 1007, 1064, 1157, 1340
BstNI CCWGG 1 cut(s) 345
BstNSI RCATGY 1 cut(s) 1160
BstSCI CCNGG 4 cut(s) 101, 343, 1181, 1182
BstSFI CTRYAG 1 cut(s) 1002
BstSLI GKGCMC 1 cut(s) 175
BstV2I GAAGAC 1 cut(s) 267
BstX2I RGATCY 1 cut(s) 964
BstXI CCANNNNNNTGG 1 cut(s) 139
BstYI RGATCY 1 cut(s) 964
BstZ17I GTATAC 1 cut(s) 1071
Bsu36I CCTNAGG 1 cut(s) 972
BsuI GTATCC 1 cut(s) 851
BsuRI GGCC 4 cut(s) 5, 101, 131, 173
BtsCI GGATG 5 cut(s) 160, 262, 659, 757, 1140
BtsI GCAGTG 4 cut(s) 36, 596, 802, 915
BtsIMutI CAGTG 5 cut(s) 36, 596, 741, 802, 915
Cac8I GCNNGC 1 cut(s) 1158
CfoI GCGC 1 cut(s) 1364
Cfr13I GGNCC 4 cut(s) 171, 172, 1090, 1294
Cfr9I CCCGGG 1 cut(s) 1182
CseI GACGC 2 cut(s) 20, 467
Csp6I GTAC 2 cut(s) 217, 1216
CviAII CATG 5 cut(s) 284, 491, 603, 1157, 1358
CviQI GTAC 2 cut(s) 217, 1216
DdeI CTNAG 3 cut(s) 204, 607, 972
DpnI GATC 4 cut(s) 84, 732, 966, 1086
DpnII GATC 4 cut(s) 82, 730, 964, 1084
EaeI YGGCCR 3 cut(s) 3, 99, 129
Eam1104I CTCTTC 2 cut(s) 381, 778
EarI CTCTTC 2 cut(s) 381, 778
Eco24I GRGCYC 2 cut(s) 175, 695
Eco47I GGWCC 2 cut(s) 1090, 1294
Eco57I CTGAAG 1 cut(s) 1005
Eco81I CCTNAGG 1 cut(s) 972
Eco88I CYCGRG 1 cut(s) 1182
EcoO109I RGGNCCY 3 cut(s) 171, 172, 1294
EcoRII CCWGG 1 cut(s) 343
EcoT22I ATGCAT 1 cut(s) 1162
EcoT38I GRGCYC 2 cut(s) 175, 695
FaeI CATG 5 cut(s) 287, 494, 606, 1160, 1361
FaqI GGGAC 1 cut(s) 1307
FatI CATG 5 cut(s) 283, 490, 602, 1156, 1357
FauI CCCGC 1 cut(s) 1284
FblI GTMKAC 2 cut(s) 895, 1070
FokI GGATG 5 cut(s) 167, 269, 646, 764, 1147
FriOI GRGCYC 2 cut(s) 175, 695
FspBI CTAG 1 cut(s) 354
GlaI GCGC 1 cut(s) 1363
GsuI CTGGAG 1 cut(s) 245
HaeII RGCGCY 1 cut(s) 1365
HaeIII GGCC 4 cut(s) 5, 101, 131, 173
HapII CCGG 2 cut(s) 102, 1183
HgaI GACGC 2 cut(s) 20, 467
HhaI GCGC 1 cut(s) 1364
Hin1II CATG 5 cut(s) 287, 494, 606, 1160, 1361
Hin6I GCGC 1 cut(s) 1362
HinP1I GCGC 1 cut(s) 1362
HincII GTYRAC 2 cut(s) 497, 859
HindII GTYRAC 2 cut(s) 497, 859
HinfI GANTC 4 cut(s) 76, 329, 619, 808
HpaII CCGG 2 cut(s) 102, 1183
HphI GGTGA 3 cut(s) 278, 740, 972
Hpy166II GTNNAC 5 cut(s) 497, 596, 859, 896, 1071
Hpy188I TCNGA 3 cut(s) 886, 1053, 1328
Hpy188III TCNNGA 4 cut(s) 80, 326, 1017, 1088
Hpy8I GTNNAC 5 cut(s) 497, 596, 859, 896, 1071
Hpy99I CGWCG 1 cut(s) 461
HpyAV CCTTC 6 cut(s) 110, 115, 185, 332, 1201, 1217
HpyCH4III ACNGT 4 cut(s) 139, 266, 856, 1242
HpyCH4IV ACGT 1 cut(s) 243
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 20, 26, 656, 1007, 1064, 1157, 1340
HpyF3I CTNAG 3 cut(s) 204, 607, 972
HpySE526I ACGT 1 cut(s) 243
Hsp92II CATG 5 cut(s) 287, 494, 606, 1160, 1361
HspAI GCGC 1 cut(s) 1362
KflI GGGWCCC 1 cut(s) 1294
KpnI GGTACC 1 cut(s) 1219
Kzo9I GATC 4 cut(s) 82, 730, 964, 1084
LguI GCTCTTC 1 cut(s) 381
LmnI GCTCC 3 cut(s) 365, 656, 690
LweI GCATC 7 cut(s) 145, 493, 668, 1018, 1054, 1090, 1309
MaeI CTAG 1 cut(s) 354
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 3 cut(s) 67, 951, 978
MalI GATC 4 cut(s) 84, 732, 966, 1086
MboI GATC 4 cut(s) 82, 730, 964, 1084
MboII GAAGA 6 cut(s) 272, 368, 795, 970, 1003, 1006
MflI RGATCY 1 cut(s) 964
MhlI GDGCHC 2 cut(s) 175, 695
MlsI TGGCCA 1 cut(s) 131
MluNI TGGCCA 1 cut(s) 131
MmeI TCCRAC 2 cut(s) 1112, 1224
Mox20I TGGCCA 1 cut(s) 131
Mph1103I ATGCAT 1 cut(s) 1162
MscI TGGCCA 1 cut(s) 131
MseI TTAA 8 cut(s) 89, 150, 444, 473, 716, 921, 930, 1190
MslI CAYNNNNRTG 1 cut(s) 601
Msp20I TGGCCA 1 cut(s) 131
MspA1I CMGCKG 2 cut(s) 8, 779
MspI CCGG 2 cut(s) 102, 1183
MspR9I CCNGG 4 cut(s) 103, 345, 1183, 1184
MvaI CCWGG 1 cut(s) 345
MwoI GCNNNNNNNGC 8 cut(s) 11, 20, 26, 656, 1007, 1064, 1157, 1340
NciI CCSGG 3 cut(s) 103, 1183, 1184
NdeII GATC 4 cut(s) 82, 730, 964, 1084
NlaIII CATG 5 cut(s) 287, 494, 606, 1160, 1361
NmeAIII GCCGAG 2 cut(s) 271, 1177
NmuCI GTSAC 1 cut(s) 978
NsiI ATGCAT 1 cut(s) 1162
NspI RCATGY 1 cut(s) 1160
PaeI GCATGC 1 cut(s) 1160
PciSI GCTCTTC 1 cut(s) 381
PfeI GAWTC 4 cut(s) 76, 329, 619, 808
PpuMI RGGWCCY 1 cut(s) 1294
Psp5II RGGWCCY 1 cut(s) 1294
Psp6I CCWGG 1 cut(s) 343
PspGI CCWGG 1 cut(s) 343
PspOMI GGGCCC 1 cut(s) 171
PspPI GGNCC 4 cut(s) 171, 172, 1090, 1294
PspPPI RGGWCCY 1 cut(s) 1294
PstI CTGCAG 1 cut(s) 1006
PsuI RGATCY 1 cut(s) 964
PvuII CAGCTG 1 cut(s) 779
RsaI GTAC 2 cut(s) 218, 1217
RsaNI GTAC 2 cut(s) 217, 1216
RseI CAYNNNNRTG 1 cut(s) 601
SapI GCTCTTC 1 cut(s) 381
SaqAI TTAA 8 cut(s) 89, 150, 444, 473, 716, 921, 930, 1190
Sau3AI GATC 4 cut(s) 82, 730, 964, 1084
Sau96I GGNCC 4 cut(s) 171, 172, 1090, 1294
ScaI AGTACT 1 cut(s) 218
ScrFI CCNGG 4 cut(s) 103, 345, 1183, 1184
SduI GDGCHC 2 cut(s) 175, 695
SfaNI GCATC 7 cut(s) 145, 493, 668, 1018, 1054, 1090, 1309
SfcI CTRYAG 1 cut(s) 1002
SinI GGWCC 2 cut(s) 1090, 1294
SmaI CCCGGG 1 cut(s) 1184
SmiMI CAYNNNNRTG 1 cut(s) 601
SmlI CTYRAG 1 cut(s) 869
SmoI CTYRAG 1 cut(s) 869
SphI GCATGC 1 cut(s) 1160
SsiI CCGC 4 cut(s) 6, 793, 1291, 1334
SspI AATATT 2 cut(s) 562, 643
SspMI CTAG 1 cut(s) 354
StyD4I CCNGG 4 cut(s) 101, 343, 1181, 1182
TaaI ACNGT 4 cut(s) 139, 266, 856, 1242
TaiI ACGT 1 cut(s) 246
TaqI TCGA 1 cut(s) 938
TatI WGTACW 1 cut(s) 216
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 4 cut(s) 76, 329, 619, 808
Tru1I TTAA 8 cut(s) 89, 150, 444, 473, 716, 921, 930, 1190
Tru9I TTAA 8 cut(s) 89, 150, 444, 473, 716, 921, 930, 1190
TscAI CASTG 5 cut(s) 36, 603, 748, 802, 915
TseFI GTSAC 1 cut(s) 978
Tsp45I GTSAC 1 cut(s) 978
TspDTI ATGAA 6 cut(s) 129, 167, 273, 368, 1272, 1346
TspGWI ACGGA 2 cut(s) 1185, 1363
TspMI CCCGGG 1 cut(s) 1182
TspRI CASTG 5 cut(s) 36, 603, 748, 802, 915
VpaK11BI GGWCC 2 cut(s) 1090, 1294
XapI RAATTY 3 cut(s) 94, 916, 1352
XceI RCATGY 1 cut(s) 1160
XmaI CCCGGG 1 cut(s) 1182
XmiI GTMKAC 2 cut(s) 895, 1070
XspI CTAG 1 cut(s) 354
ZrmI AGTACT 1 cut(s) 218
Zsp2I ATGCAT 1 cut(s) 1162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.