Rroxscaffold_6G00428310

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002248
Physical Location & Seq
Forward (+)
48759262 .. 48766382
7121 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00428310.1

Sequence Viewer

Length: 2037 bp
ATGGATATGAAAGACGTCGAGCAAATACCCGCGCCGCAGCCGGGGAAGTCATCTTTGTCCTCGGAGTCATCGCCGTGCCTGTCGCTTTCGTCCTCAGAGGAGGAGCTGCAGGACATGCCGCTGGCGCCGCCGAAGATGATGAGCCGGAAGAGGCTGTGCAAGCAGCTGTCCATGTGCGAGACGCCGCGTGACATTGCATGGGAGAAGCGGCGCCGGCAAATTCTGAAGCAGGAGAGGAAGAGGAAGAGGAACGGAAATGGTGACTCCCCCGGCGCCCCCGCCGAACCAGAGGGAGACGACATGAACCTGACCGACGAGGACTTGCATGAGCTGAAAGGGTGTATAGAGCTAGGGTTTGGGTTCAATGAGGAAGAGGGTCAACGGTTGTGCCCCACATTGCCGGCTCTTGACCTTTACTTTGCCGTTAACCGCCAGTTCTCGCCGAGTCCGATCTCCTCGCCGAATAGCAGCCGACATTCCACGTCGTCAATTGGTGGTGCGACGTCGTCGTTTGGTGGACTTTCATCGTCGTTTGGAAGCCCTAGCCCTAGGAGCGACAGTGATGGATGGAAGATATGCAGCCCAGCTAGGGAAATAGTGCAGCAGTTTCTGAATGCTGCATGTACTGGGGATGTTGATCTCTTAAAGAAGTTGGCGGGGAAACTTGATGAAGGGAAGGGATTGGCCAAAACTGTGGCTGATATTAAGGATGCCAACAAGCGCGGCGCGCTTCATTTTGCTGCAAGAGAGGGCCAGTCTGAGATGTGCAAGTACTTGCTGGAGGAATTGAAACTTGATGTTGATACCAAAGACGAAGACGGCGACACTCCCCTTATTCATGCTGCTCGCCTAGGCCATACTGATACTGCAAAACTGCTTCTTGAGTATGGTGCCAATCCGTCTATAGCCAGTGATTTAGGGGCTACAGCTCTTCATCATTCCGCTGGGCTAGGAAACATTGAGTTGCTGAGGTACTTAATTTCCAAGGGTGTCGATGTCAATTCACAAAGTGATGCTGGTTCTCCTTTAATTTGGGCTGCTGGTCATGACCAACCAGAGGCTGTGAAAGTTCTGTTAGAACACCACGCTAATCCTAATGCTGAAAGTGATGATGATATTACTCCATTGTTGTCATCTGTGGCAGCTGGTTCACTGGCGTGCTTGGAGTTATTGATTCAGGCAGGTGCCAAAGTAAATATGAGTGCTGGTGGAGCGACCCCTTTACACATTGCTGCTGATATTGGGAACTTGGAGAGTATAAAATGTTTGTTAAAAGCTGGAGCTGATCCCAATGTAACTGATGAGTTTCTTTTACTAATCGATAGACTTGCTATTGCACCGCAGGAGGGTTTGAAGCCAATACAGGTTGCAGCTGCAAGTGGGAACCGAAGCGCTGTGGAGATCCTCTTTCCCTTGACATCACAAGTTGAAACCATTTCAAAATGGACAGTTGACGGCATACTTGAGTATATGCAGTCTGAAACAAGCAGACAACAGGATTTCCTAGATTTGTCTGCCATTATTGCTGCAGAGTTGATTTCTAACTTTCAAGAGAAAAATTTGCAGGAACAACCGCGAAATACTAAGGAACTTAATGTCCCAAAAGACTCGACTTCACTGAAGAAAGAGCTCCCTGAGGTGCCAAGTGTGACACCTGAGGCAAAGAAAAGAGCCGCAGAAGCAAAATCGAGAGGAGGCGATGCTTTCAGTAGAAAGGATTATCAAGCGGCTGTTGATGCATACACGCAGGCAATTGATTTGGACCCAACTGATGCCATTTTTCTTTCCAATCGAAGCCTTTGTTGGCTTCGTCTTGGCCAAGTTGAACATGCCTTAGCTGATGCAAAGGCCTGCAGGGCATTGAAACCAGATTGGCCAAAGGCTTGCTTTCGAGAAGGTGCAGCATTGCGCCTAATGCAGGCATGGAAGCGGTTGAAGCTGGAAGGCAGTTCCACAGTAAAGATAAACAAAAACCAAAACCTTAGTTCGCCGAACGGCCACTCGTTCATCATTAAGATGCTTTTTCTTCCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 Ank_5 DUF1685 TPR_17 TPR_8

Protein Analysis

678

Amino Acids

73.36

Weight (kDa)

5.45

Isoelectric Point (pI)

53.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1685 PF07939 103 - 159 1.4e-24 Protein of unknown function (DUF1685)
Ank_2 PF12796 205 - 301 1.5e-16 Ankyrin repeats (3 copies)
Ank_4 PF13637 244 - 294 1.1e-08 Ankyrin repeats (many copies)
Ank PF00023 273 - 303 3.6e-07 Ankyrin repeat
Ank_4 PF13637 279 - 327 8.7e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 306 - 368 2.4e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 363 - 434 1e-10 Ankyrin repeats (3 copies)
Ank_4 PF13637 382 - 424 5.2e-06 Ankyrin repeats (many copies)
Ank PF00023 404 - 434 6.4e-08 Ankyrin repeat
TPR_BSK1_C PF25575 556 - 630 1.3e-12 Serine/threonine-protein kinase BSK1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1172
AatII GACGTC 2 cut(s) 18, 506
Acc36I ACCTGC 1 cut(s) 1172
AccB1I GGYRCC 6 cut(s) 124, 210, 272, 890, 1184, 1639
AccII CGCG 5 cut(s) 32, 187, 723, 728, 1576
AclWI GGATC 2 cut(s) 1280, 1396
AcoI YGGCCR 4 cut(s) 684, 1816, 1874, 1996
AcsI RAATTY 2 cut(s) 219, 1558
AcuI CTGAAG 2 cut(s) 245, 1640
AcyI GRCGYC 6 cut(s) 15, 125, 182, 211, 273, 503
AdeI CACNNNGTG 1 cut(s) 1010
AfaI GTAC 3 cut(s) 625, 773, 974
AfeI AGCGCT 1 cut(s) 1393
AfiI CCNNNNNNNGG 5 cut(s) 41, 589, 1057, 1346, 1918
AgsI TTSAA 9 cut(s) 364, 790, 1354, 1430, 1440, 1550, 1826, 1864, 1936
AjiI CACGTC 1 cut(s) 483
AjuI GAANNNNNNNTTGG 2 cut(s) 1786, 1818
AleI CACNNNNGTG 1 cut(s) 1156
AloI GAACNNNNNNTCC 2 cut(s) 1581, 1613
Alw21I GWGCWC 1 cut(s) 1632
Alw26I GTCTC 2 cut(s) 173, 288
AlwI GGATC 2 cut(s) 1280, 1396
AlwNI CAGNNNCTG 2 cut(s) 610, 1061
Aor51HI AGCGCT 1 cut(s) 1393
AoxI GGCC 7 cut(s) 684, 751, 853, 1816, 1848, 1874, 1996
ApoI RAATTY 2 cut(s) 219, 1558
ArsI GACNNNNNNTTYG 2 cut(s) 401, 433
AspA2I CCTAGG 2 cut(s) 548, 850
AspLEI GCGC 9 cut(s) 34, 127, 213, 275, 723, 728, 730, 1394, 1911
AspS9I GGNCC 2 cut(s) 751, 1762
AsuC2I CCSGG 2 cut(s) 42, 270
AsuHPI GGTGA 1 cut(s) 272
AvaII GGWCC 1 cut(s) 1762
AvrII CCTAGG 2 cut(s) 548, 850
AxyI CCTNAGG 2 cut(s) 1635, 1656
BaeGI GKGCMC 1 cut(s) 392
BalI TGGCCA 3 cut(s) 686, 1818, 1876
BanI GGYRCC 6 cut(s) 124, 210, 272, 890, 1184, 1639
BanII GRGCYC 1 cut(s) 1632
BbsI GAAGAC 1 cut(s) 822
Bbv12I GWGCWC 1 cut(s) 1632
BbvCI CCTCAGC 1 cut(s) 968
BccI CCATC 2 cut(s) 557, 561
BceAI ACGGC 5 cut(s) 58, 407, 835, 1471, 2011
BcgI CGANNNNNNTGC 2 cut(s) 1310, 1344
BcnI CCSGG 2 cut(s) 42, 270
BcoDI GTCTC 2 cut(s) 173, 288
BfaI CTAG 7 cut(s) 350, 543, 549, 588, 851, 950, 1505
BfmI CTRYAG 5 cut(s) 107, 903, 924, 1527, 1852
BfoI RGCGCY 4 cut(s) 128, 214, 276, 1395
BfuAI ACCTGC 1 cut(s) 1172
BglI GCCNNNNNGGC 1 cut(s) 1856
BlnI CCTAGG 2 cut(s) 548, 850
BmcAI AGTACT 1 cut(s) 773
Bme1390I CCNGG 2 cut(s) 42, 270
Bme18I GGWCC 1 cut(s) 1762
BmgBI CACGTC 1 cut(s) 483
BmgT120I GGNCC 2 cut(s) 751, 1762
BmiI GGNNCC 8 cut(s) 126, 212, 274, 892, 1186, 1385, 1641, 1764
BmrFI CCNGG 2 cut(s) 42, 270
BmrI ACTGGG 1 cut(s) 636
BmsI GCATC 7 cut(s) 700, 1003, 1690, 1726, 1762, 1831, 2007
BmuI ACTGGG 1 cut(s) 636
BpiI GAAGAC 1 cut(s) 822
BpmI CTGGAG 2 cut(s) 800, 1299
Bpu10I CCTNAGC 2 cut(s) 968, 1834
BpuEI CTTGAG 2 cut(s) 902, 1484
BpuMI CCSGG 2 cut(s) 42, 270
Bsa29I ATCGAT 1 cut(s) 1320
BsaBI GATNNNNATC 1 cut(s) 636
BsaHI GRCGYC 6 cut(s) 15, 125, 182, 211, 273, 503
BsaJI CCNNGG 6 cut(s) 41, 60, 268, 548, 850, 984
BsaXI ACNNNNNCTCC 2 cut(s) 544, 574
Bsc4I CCNNNNNNNGG 5 cut(s) 41, 589, 1057, 1346, 1918
Bse118I RCCGGY 2 cut(s) 213, 400
Bse1I ACTGG 5 cut(s) 433, 631, 754, 909, 1158
Bse21I CCTNAGG 2 cut(s) 1635, 1656
Bse3DI GCAATG 4 cut(s) 192, 395, 1227, 1904
Bse8I GATNNNNATC 1 cut(s) 636
BseCI ATCGAT 1 cut(s) 1320
BseDI CCNNGG 6 cut(s) 41, 60, 268, 548, 850, 984
BseGI GGATG 3 cut(s) 572, 637, 715
BseJI GATNNNNATC 1 cut(s) 636
BseLI CCNNNNNNNGG 5 cut(s) 41, 589, 1057, 1346, 1918
BseMI GCAATG 4 cut(s) 192, 395, 1227, 1904
BseMII CTCAG 5 cut(s) 108, 750, 959, 1626, 1647
BseNI ACTGG 5 cut(s) 433, 631, 754, 909, 1158
BsePI GCGCGC 1 cut(s) 726
BseRI GAGGAG 4 cut(s) 113, 116, 445, 1707
BseSI GKGCMC 1 cut(s) 392
BseYI CCCAGC 2 cut(s) 583, 944
BsgI GTGCAG 2 cut(s) 620, 1920
Bsh1236I CGCG 5 cut(s) 32, 187, 723, 728, 1576
BshFI GGCC 7 cut(s) 686, 753, 855, 1818, 1850, 1876, 1998
BshNI GGYRCC 6 cut(s) 124, 210, 272, 890, 1184, 1639
BshVI ATCGAT 1 cut(s) 1320
BsiHKAI GWGCWC 1 cut(s) 1632
BsiSI CCGG 5 cut(s) 41, 145, 214, 270, 401
BslFI GGGAC 1 cut(s) 1583
BslI CCNNNNNNNGG 5 cut(s) 41, 589, 1057, 1346, 1918
BsmAI GTCTC 2 cut(s) 173, 288
BsmBI CGTCTC 2 cut(s) 173, 288
BsmFI GGGAC 1 cut(s) 1583
BsmI GAATGC 1 cut(s) 619
BsnI GGCC 7 cut(s) 686, 753, 855, 1818, 1850, 1876, 1998
Bsp1286I GDGCHC 2 cut(s) 392, 1632
Bsp143I GATC 4 cut(s) 450, 637, 1285, 1401
BspANI GGCC 7 cut(s) 686, 753, 855, 1818, 1850, 1876, 1998
BspCNI CTCAG 5 cut(s) 107, 751, 960, 1627, 1648
BspDI ATCGAT 1 cut(s) 1320
BspFNI CGCG 5 cut(s) 32, 187, 723, 728, 1576
BspHI TCATGA 2 cut(s) 1045, 2033
BspLI GGNNCC 8 cut(s) 126, 212, 274, 892, 1186, 1385, 1641, 1764
BspMAI CTGCAG 3 cut(s) 111, 1531, 1856
BspMI ACCTGC 1 cut(s) 1172
BspPI GGATC 2 cut(s) 1280, 1396
BspQI GCTCTTC 1 cut(s) 936
BspT107I GGYRCC 6 cut(s) 124, 210, 272, 890, 1184, 1639
BsrDI GCAATG 4 cut(s) 192, 395, 1227, 1904
BsrFI RCCGGY 2 cut(s) 213, 400
BsrI ACTGG 5 cut(s) 433, 631, 754, 909, 1158
BssAI RCCGGY 2 cut(s) 213, 400
BssECI CCNNGG 6 cut(s) 41, 60, 268, 548, 850, 984
BssHII GCGCGC 1 cut(s) 726
BssMI GATC 4 cut(s) 450, 637, 1285, 1401
BssNI GRCGYC 6 cut(s) 15, 125, 182, 211, 273, 503
BssT1I CCWWGG 3 cut(s) 548, 850, 984
Bst4CI ACNGT 5 cut(s) 384, 560, 694, 1450, 1957
Bst6I CTCTTC 5 cut(s) 143, 233, 239, 366, 936
BstACI GRCGYC 6 cut(s) 15, 125, 182, 211, 273, 503
BstAPI GCANNNNNTGC 1 cut(s) 115
BstDEI CTNAG 8 cut(s) 94, 759, 968, 1584, 1635, 1656, 1834, 1982
BstENI CCTNNNNNAGG 1 cut(s) 1916
BstF5I GGATG 3 cut(s) 572, 637, 715
BstFNI CGCG 5 cut(s) 32, 187, 723, 728, 1576
BstH2I RGCGCY 4 cut(s) 128, 214, 276, 1395
BstHHI GCGC 9 cut(s) 34, 127, 213, 275, 723, 728, 730, 1394, 1911
BstKTI GATC 4 cut(s) 453, 640, 1288, 1404
BstMAI GTCTC 2 cut(s) 173, 288
BstMBI GATC 4 cut(s) 450, 637, 1285, 1401
BstNSI RCATGY 3 cut(s) 118, 624, 1832
BstSCI CCNGG 2 cut(s) 40, 268
BstSFI CTRYAG 5 cut(s) 107, 903, 924, 1527, 1852
BstSLI GKGCMC 1 cut(s) 392
BstUI CGCG 5 cut(s) 32, 187, 723, 728, 1576
BstV2I GAAGAC 1 cut(s) 822
BstX2I RGATCY 1 cut(s) 1401
BstXI CCANNNNNNTGG 1 cut(s) 694
BstYI RGATCY 1 cut(s) 1401
Bsu15I ATCGAT 1 cut(s) 1320
Bsu36I CCTNAGG 2 cut(s) 1635, 1656
BsuRI GGCC 7 cut(s) 686, 753, 855, 1818, 1850, 1876, 1998
BsuTUI ATCGAT 1 cut(s) 1320
BtgZI GCGATG 2 cut(s) 54, 1713
BtrI CACGTC 1 cut(s) 483
BtsCI GGATG 3 cut(s) 572, 637, 715
BtsIMutI CAGTG 4 cut(s) 565, 916, 1151, 1616
BveI ACCTGC 1 cut(s) 1172
CaiI CAGNNNCTG 2 cut(s) 610, 1061
CciI TCATGA 2 cut(s) 1045, 2033
CfoI GCGC 9 cut(s) 34, 127, 213, 275, 723, 728, 730, 1394, 1911
Cfr10I RCCGGY 2 cut(s) 213, 400
Cfr13I GGNCC 2 cut(s) 751, 1762
ClaI ATCGAT 1 cut(s) 1320
CseI GACGC 1 cut(s) 190
Csp6I GTAC 3 cut(s) 624, 772, 973
CviQI GTAC 3 cut(s) 624, 772, 973
DdeI CTNAG 8 cut(s) 94, 759, 968, 1584, 1635, 1656, 1834, 1982
DinI GGCGCC 3 cut(s) 126, 212, 274
DpnI GATC 4 cut(s) 452, 639, 1287, 1403
DpnII GATC 4 cut(s) 450, 637, 1285, 1401
DraIII CACNNNGTG 1 cut(s) 1010
EaeI YGGCCR 4 cut(s) 684, 1816, 1874, 1996
Eam1104I CTCTTC 5 cut(s) 143, 233, 239, 366, 936
EarI CTCTTC 5 cut(s) 143, 233, 239, 366, 936
Ecl136II GAGCTC 1 cut(s) 1630
Eco130I CCWWGG 3 cut(s) 548, 850, 984
Eco147I AGGCCT 1 cut(s) 1850
Eco24I GRGCYC 1 cut(s) 1632
Eco47I GGWCC 1 cut(s) 1762
Eco47III AGCGCT 1 cut(s) 1393
Eco53kI GAGCTC 1 cut(s) 1630
Eco57I CTGAAG 2 cut(s) 245, 1640
Eco81I CCTNAGG 2 cut(s) 1635, 1656
EcoICRI GAGCTC 1 cut(s) 1630
EcoNI CCTNNNNNAGG 1 cut(s) 1916
EcoT14I CCWWGG 3 cut(s) 548, 850, 984
EcoT22I ATGCAT 1 cut(s) 1741
EcoT38I GRGCYC 1 cut(s) 1632
EgeI GGCGCC 3 cut(s) 126, 212, 274
EheI GGCGCC 3 cut(s) 126, 212, 274
ErhI CCWWGG 3 cut(s) 548, 850, 984
Esp3I CGTCTC 2 cut(s) 173, 288
FalI AAGNNNNNCTT 2 cut(s) 1871, 1903
FaqI GGGAC 1 cut(s) 1583
FauI CCCGC 3 cut(s) 37, 286, 649
FokI GGATG 3 cut(s) 579, 644, 722
FriOI GRGCYC 1 cut(s) 1632
FspBI CTAG 7 cut(s) 350, 543, 549, 588, 851, 950, 1505
GlaI GCGC 9 cut(s) 33, 126, 212, 274, 722, 727, 729, 1393, 1910
GsaI CCCAGC 2 cut(s) 587, 948
GsuI CTGGAG 2 cut(s) 800, 1299
HaeII RGCGCY 4 cut(s) 128, 214, 276, 1395
HaeIII GGCC 7 cut(s) 686, 753, 855, 1818, 1850, 1876, 1998
HapII CCGG 5 cut(s) 41, 145, 214, 270, 401
HgaI GACGC 1 cut(s) 190
HhaI GCGC 9 cut(s) 34, 127, 213, 275, 723, 728, 730, 1394, 1911
Hin1I GRCGYC 6 cut(s) 15, 125, 182, 211, 273, 503
Hin6I GCGC 9 cut(s) 32, 125, 211, 273, 721, 726, 728, 1392, 1909
HinP1I GCGC 9 cut(s) 32, 125, 211, 273, 721, 726, 728, 1392, 1909
HincII GTYRAC 3 cut(s) 380, 427, 1453
HindII GTYRAC 3 cut(s) 380, 427, 1453
HinfI GANTC 5 cut(s) 65, 263, 445, 1174, 1607
HpaI GTTAAC 1 cut(s) 427
HpaII CCGG 5 cut(s) 41, 145, 214, 270, 401
HphI GGTGA 1 cut(s) 272
Hpy166II GTNNAC 5 cut(s) 380, 427, 518, 1151, 1453
Hpy188I TCNGA 7 cut(s) 64, 97, 225, 450, 612, 760, 1480
Hpy188III TCNNGA 7 cut(s) 407, 881, 1046, 1550, 1689, 1892, 2034
Hpy8I GTNNAC 5 cut(s) 380, 427, 518, 1151, 1453
Hpy99I CGWCG 7 cut(s) 20, 317, 487, 505, 508, 511, 532
HpyAV CCTTC 4 cut(s) 665, 670, 1889, 1937
HpyCH4III ACNGT 5 cut(s) 384, 560, 694, 1450, 1957
HpyCH4IV ACGT 3 cut(s) 15, 482, 503
HpyF3I CTNAG 8 cut(s) 94, 759, 968, 1584, 1635, 1656, 1834, 1982
HpySE526I ACGT 3 cut(s) 15, 482, 503
Hsp92I GRCGYC 6 cut(s) 15, 125, 182, 211, 273, 503
HspAI GCGC 9 cut(s) 32, 125, 211, 273, 721, 726, 728, 1392, 1909
KasI GGCGCC 3 cut(s) 124, 210, 272
KroI GCCGGC 2 cut(s) 213, 400
KroNI GCCGGC 2 cut(s) 215, 402
KspAI GTTAAC 1 cut(s) 427
Kzo9I GATC 4 cut(s) 450, 637, 1285, 1401
LguI GCTCTTC 1 cut(s) 936
LmnI GCTCC 5 cut(s) 103, 552, 1211, 1280, 1635
LweI GCATC 7 cut(s) 700, 1003, 1690, 1726, 1762, 1831, 2007
MaeI CTAG 7 cut(s) 350, 543, 549, 588, 851, 950, 1505
MaeII ACGT 3 cut(s) 15, 482, 503
MaeIII GTNAC 4 cut(s) 188, 260, 1294, 1648
MalI GATC 4 cut(s) 452, 639, 1287, 1403
MboI GATC 4 cut(s) 450, 637, 1285, 1401
MfeI CAATTG 2 cut(s) 489, 1752
MflI RGATCY 1 cut(s) 1401
MhlI GDGCHC 2 cut(s) 392, 1632
MlsI TGGCCA 3 cut(s) 686, 1818, 1876
MluCI AATT 8 cut(s) 219, 489, 785, 978, 1000, 1029, 1558, 1752
MluNI TGGCCA 3 cut(s) 686, 1818, 1876
Mly113I GGCGCC 3 cut(s) 125, 211, 273
MlyI GAGTC 4 cut(s) 74, 257, 454, 1601
Mox20I TGGCCA 3 cut(s) 686, 1818, 1876
Mph1103I ATGCAT 1 cut(s) 1741
MroNI GCCGGC 2 cut(s) 213, 400
MscI TGGCCA 3 cut(s) 686, 1818, 1876
MseI TTAA 8 cut(s) 426, 644, 705, 977, 1028, 1271, 1593, 2013
MslI CAYNNNNRTG 3 cut(s) 73, 1156, 2015
Msp20I TGGCCA 3 cut(s) 686, 1818, 1876
MspA1I CMGCKG 5 cut(s) 121, 166, 944, 1145, 1373
MspI CCGG 5 cut(s) 41, 145, 214, 270, 401
MspR9I CCNGG 2 cut(s) 42, 270
MteI GCGCNGCGC 1 cut(s) 724
MunI CAATTG 2 cut(s) 489, 1752
Mva1269I GAATGC 1 cut(s) 619
MvnI CGCG 5 cut(s) 32, 187, 723, 728, 1576
NaeI GCCGGC 2 cut(s) 215, 402
NarI GGCGCC 3 cut(s) 125, 211, 273
NciI CCSGG 2 cut(s) 42, 270
NdeII GATC 4 cut(s) 450, 637, 1285, 1401
NgoMIV GCCGGC 2 cut(s) 213, 400
NlaIV GGNNCC 8 cut(s) 126, 212, 274, 892, 1186, 1385, 1641, 1764
NmeAIII GCCGAG 1 cut(s) 468
NmuCI GTSAC 3 cut(s) 188, 260, 1648
NsiI ATGCAT 1 cut(s) 1741
NspI RCATGY 3 cut(s) 118, 624, 1832
OliI CACNNNNGTG 1 cut(s) 1156
PagI TCATGA 2 cut(s) 1045, 2033
PaqCI CACCTGC 1 cut(s) 1172
PauI GCGCGC 1 cut(s) 726
PceI AGGCCT 1 cut(s) 1850
PciSI GCTCTTC 1 cut(s) 936
PcsI WCGNNNNNNNCGW 2 cut(s) 446, 819
PctI GAATGC 1 cut(s) 619
PdiI GCCGGC 2 cut(s) 215, 402
PfeI GAWTC 1 cut(s) 1174
PflFI GACNNNGTC 1 cut(s) 505
PleI GAGTC 4 cut(s) 73, 257, 453, 1601
PluTI GGCGCC 3 cut(s) 128, 214, 276
PpsI GAGTC 4 cut(s) 73, 257, 453, 1601
Psp124BI GAGCTC 1 cut(s) 1632
PspFI CCCAGC 2 cut(s) 583, 944
PspN4I GGNNCC 8 cut(s) 126, 212, 274, 892, 1186, 1385, 1641, 1764
PspPI GGNCC 2 cut(s) 751, 1762
PstI CTGCAG 3 cut(s) 111, 1531, 1856
PstNI CAGNNNCTG 2 cut(s) 610, 1061
PsuI RGATCY 1 cut(s) 1401
PsyI GACNNNGTC 1 cut(s) 505
PteI GCGCGC 1 cut(s) 726
PvuII CAGCTG 3 cut(s) 166, 1145, 1373
RsaI GTAC 3 cut(s) 625, 773, 974
RsaNI GTAC 3 cut(s) 624, 772, 973
RseI CAYNNNNRTG 3 cut(s) 73, 1156, 2015
SacI GAGCTC 1 cut(s) 1632
SapI GCTCTTC 1 cut(s) 936
SaqAI TTAA 8 cut(s) 426, 644, 705, 977, 1028, 1271, 1593, 2013
Sau3AI GATC 4 cut(s) 450, 637, 1285, 1401
Sau96I GGNCC 2 cut(s) 751, 1762
SbfI CCTGCAGG 1 cut(s) 1856
ScaI AGTACT 1 cut(s) 773
SchI GAGTC 4 cut(s) 74, 257, 454, 1601
ScrFI CCNGG 2 cut(s) 42, 270
SdaI CCTGCAGG 1 cut(s) 1856
SduI GDGCHC 2 cut(s) 392, 1632
SfaNI GCATC 7 cut(s) 700, 1003, 1690, 1726, 1762, 1831, 2007
SfcI CTRYAG 5 cut(s) 107, 903, 924, 1527, 1852
SfoI GGCGCC 3 cut(s) 126, 212, 274
SinI GGWCC 1 cut(s) 1762
SmiMI CAYNNNNRTG 3 cut(s) 73, 1156, 2015
SmlI CTYRAG 2 cut(s) 881, 1463
SmoI CTYRAG 2 cut(s) 881, 1463
Sse8387I CCTGCAGG 1 cut(s) 1856
Sse9I AATT 8 cut(s) 219, 489, 785, 978, 1000, 1029, 1558, 1752
SseBI AGGCCT 1 cut(s) 1850
SspDI GGCGCC 3 cut(s) 124, 210, 272
SspMI CTAG 7 cut(s) 350, 543, 549, 588, 851, 950, 1505
SstI GAGCTC 1 cut(s) 1632
StuI AGGCCT 1 cut(s) 1850
StyD4I CCNGG 2 cut(s) 40, 268
StyI CCWWGG 3 cut(s) 548, 850, 984
TaaI ACNGT 5 cut(s) 384, 560, 694, 1450, 1957
TaiI ACGT 3 cut(s) 18, 485, 506
TaqI TCGA 7 cut(s) 18, 993, 1320, 1610, 1688, 1792, 1891
TaqII GACCGA 1 cut(s) 326
TasI AATT 8 cut(s) 219, 489, 785, 978, 1000, 1029, 1558, 1752
TatI WGTACW 2 cut(s) 623, 771
TauI GCSGC 8 cut(s) 37, 121, 130, 187, 211, 726, 1676, 1730
TfiI GAWTC 1 cut(s) 1174
Tru1I TTAA 8 cut(s) 426, 644, 705, 977, 1028, 1271, 1593, 2013
Tru9I TTAA 8 cut(s) 426, 644, 705, 977, 1028, 1271, 1593, 2013
TscAI CASTG 4 cut(s) 565, 916, 1158, 1623
TseFI GTSAC 3 cut(s) 188, 260, 1648
Tsp45I GTSAC 3 cut(s) 188, 260, 1648
TspDTI ATGAA 9 cut(s) 23, 317, 513, 684, 722, 827, 923, 1996, 2022
TspGWI ACGGA 2 cut(s) 267, 888
TspRI CASTG 4 cut(s) 565, 916, 1158, 1623
Tth111I GACNNNGTC 1 cut(s) 505
VpaK11BI GGWCC 1 cut(s) 1762
XagI CCTNNNNNAGG 1 cut(s) 1916
XapI RAATTY 2 cut(s) 219, 1558
XceI RCATGY 3 cut(s) 118, 624, 1832
XmaJI CCTAGG 2 cut(s) 548, 850
XspI CTAG 7 cut(s) 350, 543, 549, 588, 851, 950, 1505
ZraI GACGTC 2 cut(s) 16, 504
ZrmI AGTACT 1 cut(s) 773
Zsp2I ATGCAT 1 cut(s) 1741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.