RLG00000035453

ankyrin repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002248
Physical Location & Seq
Forward (+)
67073219 .. 67075464
2246 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035453

Sequence Viewer

Length: 555 bp
ATGTGCAAGTACTTGCTGGAGGAATTGAAAATTGATGTTGATACCAAAGATGAAGATGGCGACACTCCCCTTATTCATGCTGCTCACCTAGGCCATACTGATACTGCCAAACTGCTTCTTGAGTATGGTGCCAATTCGTCTATAGCCAGTGATTTAGGGGCTACAGCTCTTCATCATTCCACTGGACTAGGAAACATTGAGTTGCTGAGGTACTTAATTTCCAAGTGTGTTGATGTCAATTCACAAAGTGATGCTGGTTCTCCTTTAATTTGGGCTGCTGGTCATGACCAACTAGATGCTGTGAAAGTTCTGTTAGAACACCAGGCTAATCCTAATGTTGAAAGTGATGATGATATTACTCCATTGTTGTCATCTGTGGCAGCTGGTTCACTGGCATGCTTGGAGTTATTGATTCAGGCAGGTGCCAAAGTAAATATGAGTGCTGGTGGAGCGACCCCTTTACACATCGCTGCTGACATTGAGAACTTGGAGATTATAAAATGTTTGTTAAAAGCTGGAGCTGATCCCAGTGTAACTGATGAGGTGAGGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 Ank_5 DUF1685 TPR_17 TPR_8

Protein Analysis

185

Amino Acids

19.34

Weight (kDa)

4.53

Isoelectric Point (pI)

27.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 1 - 45 4.8e-08 Ankyrin repeats (3 copies)
Ank PF00023 19 - 47 5.9e-07 Ankyrin repeat
Ank_4 PF13637 24 - 73 9.4e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 52 - 114 3.5e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 89 - 180 3e-16 Ankyrin repeats (3 copies)
Ank_4 PF13637 89 - 138 1.3e-06 Ankyrin repeats (many copies)
Ank_4 PF13637 128 - 170 9.6e-07 Ankyrin repeats (many copies)
Ank PF00023 150 - 180 9.1e-08 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 497
AarI CACCTGC 1 cut(s) 410
Acc36I ACCTGC 1 cut(s) 410
AccB1I GGYRCC 2 cut(s) 128, 422
AclWI GGATC 1 cut(s) 518
AdeI CACNNNGTG 1 cut(s) 248
AfaI GTAC 2 cut(s) 11, 212
AgsI TTSAA 2 cut(s) 28, 341
AjnI CCWGG 1 cut(s) 321
AluBI AGCT 4 cut(s) 167, 383, 515, 521
AluI AGCT 4 cut(s) 167, 383, 515, 521
AlwI GGATC 1 cut(s) 518
AoxI GGCC 1 cut(s) 91
ApeKI GCWGC 4 cut(s) 80, 275, 380, 470
AspA2I CCTAGG 1 cut(s) 88
AsuHPI GGTGA 1 cut(s) 77
AvrII CCTAGG 1 cut(s) 88
BanI GGYRCC 2 cut(s) 128, 422
BbvCI CCTCAGC 1 cut(s) 206
BbvI GCAGC 4 cut(s) 67, 262, 392, 457
BccI CCATC 1 cut(s) 50
BciT130I CCWGG 1 cut(s) 323
BfaI CTAG 3 cut(s) 89, 188, 293
BfmI CTRYAG 2 cut(s) 141, 162
BfuAI ACCTGC 1 cut(s) 410
BisI GCNGC 4 cut(s) 81, 276, 381, 471
BlnI CCTAGG 1 cut(s) 88
BlsI GCNGC 4 cut(s) 82, 277, 382, 472
BmcAI AGTACT 1 cut(s) 11
Bme1390I CCNGG 1 cut(s) 323
BmiI GGNNCC 2 cut(s) 130, 424
BmrFI CCNGG 1 cut(s) 323
BmrI ACTGGG 1 cut(s) 522
BmsI GCATC 2 cut(s) 241, 286
BmuI ACTGGG 1 cut(s) 522
BpmI CTGGAG 2 cut(s) 38, 537
Bpu10I CCTNAGC 1 cut(s) 206
BpuEI CTTGAG 1 cut(s) 140
BsaJI CCNNGG 1 cut(s) 88
Bse1I ACTGG 4 cut(s) 147, 187, 396, 528
BseBI CCWGG 1 cut(s) 323
BseDI CCNNGG 1 cut(s) 88
BseMII CTCAG 1 cut(s) 197
BseNI ACTGG 4 cut(s) 147, 187, 396, 528
BseXI GCAGC 4 cut(s) 67, 262, 392, 457
BshFI GGCC 1 cut(s) 93
BshNI GGYRCC 2 cut(s) 128, 422
BsnI GGCC 1 cut(s) 93
Bsp143I GATC 1 cut(s) 523
BspANI GGCC 1 cut(s) 93
BspCNI CTCAG 1 cut(s) 198
BspHI TCATGA 1 cut(s) 283
BspLI GGNNCC 2 cut(s) 130, 424
BspMI ACCTGC 1 cut(s) 410
BspPI GGATC 1 cut(s) 518
BspQI GCTCTTC 1 cut(s) 174
BspT107I GGYRCC 2 cut(s) 128, 422
BsrI ACTGG 4 cut(s) 147, 187, 396, 528
BssECI CCNNGG 1 cut(s) 88
BssMI GATC 1 cut(s) 523
BssT1I CCWWGG 1 cut(s) 88
Bst2UI CCWGG 1 cut(s) 323
Bst6I CTCTTC 1 cut(s) 174
BstC8I GCNNGC 1 cut(s) 397
BstDEI CTNAG 1 cut(s) 206
BstKTI GATC 1 cut(s) 526
BstMBI GATC 1 cut(s) 523
BstMWI GCNNNNNNNGC 1 cut(s) 449
BstNI CCWGG 1 cut(s) 323
BstNSI RCATGY 1 cut(s) 399
BstSCI CCNGG 1 cut(s) 321
BstSFI CTRYAG 2 cut(s) 141, 162
BstV1I GCAGC 4 cut(s) 67, 262, 392, 457
BsuRI GGCC 1 cut(s) 93
BtgZI GCGATG 1 cut(s) 451
BtsIMutI CAGTG 4 cut(s) 154, 180, 389, 535
BveI ACCTGC 1 cut(s) 410
Cac8I GCNNGC 1 cut(s) 397
CciI TCATGA 1 cut(s) 283
Csp6I GTAC 2 cut(s) 10, 211
CviAII CATG 3 cut(s) 77, 284, 396
CviQI GTAC 2 cut(s) 10, 211
DdeI CTNAG 1 cut(s) 206
DpnI GATC 1 cut(s) 525
DpnII GATC 1 cut(s) 523
DraIII CACNNNGTG 1 cut(s) 248
Eam1104I CTCTTC 1 cut(s) 174
EarI CTCTTC 1 cut(s) 174
Eco130I CCWWGG 1 cut(s) 88
EcoRII CCWGG 1 cut(s) 321
EcoT14I CCWWGG 1 cut(s) 88
ErhI CCWWGG 1 cut(s) 88
FaeI CATG 3 cut(s) 80, 287, 399
FaiI YATR 9 cut(s) 78, 96, 126, 143, 285, 397, 437, 497, 553
FatI CATG 3 cut(s) 76, 283, 395
Fnu4HI GCNGC 4 cut(s) 81, 276, 381, 471
Fsp4HI GCNGC 4 cut(s) 81, 276, 381, 471
FspBI CTAG 3 cut(s) 89, 188, 293
GluI GCNGC 4 cut(s) 81, 276, 381, 471
GsuI CTGGAG 2 cut(s) 38, 537
HaeIII GGCC 1 cut(s) 93
Hin1II CATG 3 cut(s) 80, 287, 399
HinfI GANTC 1 cut(s) 412
HphI GGTGA 1 cut(s) 77
Hpy166II GTNNAC 1 cut(s) 389
Hpy188III TCNNGA 2 cut(s) 119, 284
Hpy8I GTNNAC 1 cut(s) 389
HpyCH4V TGCA 1 cut(s) 6
HpyF10VI GCNNNNNNNGC 1 cut(s) 449
HpyF3I CTNAG 1 cut(s) 206
Hsp92II CATG 3 cut(s) 80, 287, 399
Kzo9I GATC 1 cut(s) 523
LguI GCTCTTC 1 cut(s) 174
LmnI GCTCC 2 cut(s) 449, 518
Lsp1109I GCAGC 4 cut(s) 67, 262, 392, 457
LweI GCATC 2 cut(s) 241, 286
MaeI CTAG 3 cut(s) 89, 188, 293
MaeIII GTNAC 1 cut(s) 532
MalI GATC 1 cut(s) 525
MboI GATC 1 cut(s) 523
MboII GAAGA 2 cut(s) 65, 161
MluCI AATT 6 cut(s) 23, 30, 133, 216, 238, 267
MnlI CCTC 4 cut(s) 13, 201, 535, 540
MseI TTAA 3 cut(s) 215, 266, 509
MslI CAYNNNNRTG 1 cut(s) 394
MspA1I CMGCKG 1 cut(s) 383
MspR9I CCNGG 1 cut(s) 323
MvaI CCWGG 1 cut(s) 323
MwoI GCNNNNNNNGC 1 cut(s) 449
NdeII GATC 1 cut(s) 523
NlaIII CATG 3 cut(s) 80, 287, 399
NlaIV GGNNCC 2 cut(s) 130, 424
NspI RCATGY 1 cut(s) 399
PaeI GCATGC 1 cut(s) 399
PagI TCATGA 1 cut(s) 283
PaqCI CACCTGC 1 cut(s) 410
PciSI GCTCTTC 1 cut(s) 174
PfeI GAWTC 1 cut(s) 412
PkrI GCNGC 4 cut(s) 82, 277, 382, 472
PsiI TTATAA 1 cut(s) 497
Psp6I CCWGG 1 cut(s) 321
PspGI CCWGG 1 cut(s) 321
PspN4I GGNNCC 2 cut(s) 130, 424
PvuII CAGCTG 1 cut(s) 383
RsaI GTAC 2 cut(s) 11, 212
RsaNI GTAC 2 cut(s) 10, 211
RseI CAYNNNNRTG 1 cut(s) 394
SapI GCTCTTC 1 cut(s) 174
SaqAI TTAA 3 cut(s) 215, 266, 509
SatI GCNGC 4 cut(s) 81, 276, 381, 471
Sau3AI GATC 1 cut(s) 523
ScaI AGTACT 1 cut(s) 11
ScrFI CCNGG 1 cut(s) 323
SetI ASST 8 cut(s) 90, 169, 212, 385, 424, 517, 523, 546
SfaNI GCATC 2 cut(s) 241, 286
SfcI CTRYAG 2 cut(s) 141, 162
SmiMI CAYNNNNRTG 1 cut(s) 394
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
SphI GCATGC 1 cut(s) 399
Sse9I AATT 6 cut(s) 23, 30, 133, 216, 238, 267
SspMI CTAG 3 cut(s) 89, 188, 293
StyD4I CCNGG 1 cut(s) 321
StyI CCWWGG 1 cut(s) 88
TasI AATT 6 cut(s) 23, 30, 133, 216, 238, 267
TatI WGTACW 1 cut(s) 9
TfiI GAWTC 1 cut(s) 412
Tru1I TTAA 3 cut(s) 215, 266, 509
Tru9I TTAA 3 cut(s) 215, 266, 509
TscAI CASTG 4 cut(s) 154, 187, 396, 535
TseI GCWGC 4 cut(s) 80, 275, 380, 470
TspDTI ATGAA 3 cut(s) 65, 66, 161
TspRI CASTG 4 cut(s) 154, 187, 396, 535
XceI RCATGY 1 cut(s) 399
XmaJI CCTAGG 1 cut(s) 88
XspI CTAG 3 cut(s) 89, 188, 293
ZrmI AGTACT 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.