MD12G1243100.v1.1

ankyrin repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002248
Physical Location & Seq
Reverse (-)
31391347 .. 31393897
2551 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1243100.v1.1.491

Sequence Viewer

Length: 552 bp
ATGTTGATACAAAGGACGAAGACGGTGAGACTGCTCTTGTTCATGCTGCCCCGGCAATGCCATACCGATACTGCAAAATACCTTCTTGATTCTGGTGCCAGTCTTGGTACAGCTAGTGAATTGGGAGCCGCAGCTCTTCATCATGCTGCTGGATTAGGAGACATTGAGTTGCTGAGGTATTTGATTTCCAAAGGTGCTGATGTTAATTCACAAAGTGACGCTGGAAGTCCTTTGATATGGGCTGCTGGTCATGGACAACAAGATGCCACGAAAATTTTGTTCGAACACCATGCTAATCCAAATGCTGAAAATGATGACAGTATTACTCCTTTGTTGTCATCTGTTGCGGTTGGTATTTCCTATATGACTTATATGACGCGGGAATGTGAGACCCTCTGCCTTCTATGGTCATCATCCTCATCCTCCTCCATTACGAAAGCCTCATCTCCATCTCCACCTTTCTCGAGATTGACCAATTCTTCCTGTTATGCCAACAATCTTTTCTGTTGCTCCTCAAACTGTTTATACACTCTCTTGAAGAAGTCATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 Ank_5 DUF1685 TPR_17 TPR_8

Protein Analysis

184

Amino Acids

19.66

Weight (kDa)

6.57

Isoelectric Point (pI)

51.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 20 - 103 7.1e-14 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 95
AccII CGCG 1 cut(s) 379
AciI CCGC 3 cut(s) 129, 347, 379
AcsI RAATTY 1 cut(s) 273
AdeI CACNNNGTG 1 cut(s) 215
AfaI GTAC 1 cut(s) 109
AgsI TTSAA 1 cut(s) 538
AjuI GAANNNNNNNTTGG 2 cut(s) 485, 517
AluBI AGCT 2 cut(s) 113, 134
AluI AGCT 2 cut(s) 113, 134
Alw26I GTCTC 3 cut(s) 22, 153, 383
Ama87I CYCGRG 1 cut(s) 463
ApeKI GCWGC 4 cut(s) 46, 131, 146, 242
ApoI RAATTY 1 cut(s) 273
AsuC2I CCSGG 1 cut(s) 52
AsuHPI GGTGA 1 cut(s) 37
AsuII TTCGAA 1 cut(s) 282
AvaI CYCGRG 1 cut(s) 463
BanI GGYRCC 1 cut(s) 95
BbsI GAAGAC 1 cut(s) 26
BbvCI CCTCAGC 1 cut(s) 173
BbvI GCAGC 4 cut(s) 33, 133, 143, 229
BccI CCATC 1 cut(s) 457
BcgI CGANNNNNNTGC 2 cut(s) 272, 306
BcnI CCSGG 1 cut(s) 52
BcoDI GTCTC 3 cut(s) 22, 153, 383
BfaI CTAG 1 cut(s) 114
BisI GCNGC 5 cut(s) 47, 129, 132, 147, 243
BlsI GCNGC 5 cut(s) 48, 130, 133, 148, 244
Bme1390I CCNGG 1 cut(s) 52
BmeT110I CYCGRG 1 cut(s) 463
BmiI GGNNCC 2 cut(s) 97, 127
BmrFI CCNGG 1 cut(s) 52
BmsI GCATC 1 cut(s) 253
BpiI GAAGAC 1 cut(s) 26
Bpu10I CCTNAGC 1 cut(s) 173
Bpu14I TTCGAA 1 cut(s) 282
BpuMI CCSGG 1 cut(s) 52
BsaI GGTCTC 1 cut(s) 383
BsaJI CCNNGG 1 cut(s) 50
Bse1I ACTGG 1 cut(s) 99
Bse3DI GCAATG 1 cut(s) 62
BseDI CCNNGG 1 cut(s) 50
BseGI GGATG 2 cut(s) 413, 419
BseMI GCAATG 1 cut(s) 62
BseMII CTCAG 1 cut(s) 164
BseNI ACTGG 1 cut(s) 99
BseRI GAGGAG 2 cut(s) 415, 502
BseXI GCAGC 4 cut(s) 33, 133, 143, 229
Bsh1236I CGCG 1 cut(s) 379
BshNI GGYRCC 1 cut(s) 95
BsiHKCI CYCGRG 1 cut(s) 463
BsiSI CCGG 1 cut(s) 52
BsmAI GTCTC 3 cut(s) 22, 153, 383
Bso31I GGTCTC 1 cut(s) 383
BsoBI CYCGRG 1 cut(s) 463
Bsp119I TTCGAA 1 cut(s) 282
BspACI CCGC 3 cut(s) 129, 347, 379
BspCNI CTCAG 1 cut(s) 165
BspFNI CGCG 1 cut(s) 379
BspLI GGNNCC 2 cut(s) 97, 127
BspQI GCTCTTC 1 cut(s) 141
BspT104I TTCGAA 1 cut(s) 282
BspT107I GGYRCC 1 cut(s) 95
BspTNI GGTCTC 1 cut(s) 383
BsrDI GCAATG 1 cut(s) 62
BsrI ACTGG 1 cut(s) 99
BssECI CCNNGG 1 cut(s) 50
Bst4CI ACNGT 3 cut(s) 25, 320, 521
Bst6I CTCTTC 1 cut(s) 141
BstBI TTCGAA 1 cut(s) 282
BstDEI CTNAG 1 cut(s) 173
BstF5I GGATG 2 cut(s) 413, 419
BstFNI CGCG 1 cut(s) 379
BstMAI GTCTC 3 cut(s) 22, 153, 383
BstMWI GCNNNNNNNGC 1 cut(s) 52
BstSCI CCNGG 1 cut(s) 50
BstUI CGCG 1 cut(s) 379
BstV1I GCAGC 4 cut(s) 33, 133, 143, 229
BstV2I GAAGAC 1 cut(s) 26
BtsCI GGATG 2 cut(s) 413, 419
CseI GACGC 2 cut(s) 227, 385
Csp6I GTAC 1 cut(s) 108
CviAII CATG 4 cut(s) 43, 143, 251, 290
CviJI RGCY 5 cut(s) 113, 128, 134, 242, 440
CviKI_1 RGCY 5 cut(s) 113, 128, 134, 242, 440
CviQI GTAC 1 cut(s) 108
DdeI CTNAG 1 cut(s) 173
DraIII CACNNNGTG 1 cut(s) 215
Eam1104I CTCTTC 1 cut(s) 141
EarI CTCTTC 1 cut(s) 141
Eco31I GGTCTC 1 cut(s) 383
Eco88I CYCGRG 1 cut(s) 463
FaeI CATG 4 cut(s) 46, 146, 254, 293
FatI CATG 4 cut(s) 42, 142, 250, 289
FauI CCCGC 1 cut(s) 372
Fnu4HI GCNGC 5 cut(s) 47, 129, 132, 147, 243
FokI GGATG 2 cut(s) 400, 406
Fsp4HI GCNGC 5 cut(s) 47, 129, 132, 147, 243
FspBI CTAG 1 cut(s) 114
GluI GCNGC 5 cut(s) 47, 129, 132, 147, 243
HapII CCGG 1 cut(s) 52
HgaI GACGC 2 cut(s) 227, 385
Hin1II CATG 4 cut(s) 46, 146, 254, 293
HinfI GANTC 1 cut(s) 89
HpaII CCGG 1 cut(s) 52
HphI GGTGA 1 cut(s) 37
Hpy188III TCNNGA 4 cut(s) 86, 463, 465, 535
HpyAV CCTTC 2 cut(s) 92, 410
HpyCH4III ACNGT 3 cut(s) 25, 320, 521
HpyCH4V TGCA 1 cut(s) 74
HpyF10VI GCNNNNNNNGC 1 cut(s) 52
HpyF3I CTNAG 1 cut(s) 173
Hsp92II CATG 4 cut(s) 46, 146, 254, 293
LguI GCTCTTC 1 cut(s) 141
LmnI GCTCC 2 cut(s) 125, 515
LpnPI CCDG 7 cut(s) 65, 78, 112, 135, 207, 231, 496
Lsp1109I GCAGC 4 cut(s) 33, 133, 143, 229
LweI GCATC 1 cut(s) 253
MaeI CTAG 1 cut(s) 114
MaeIII GTNAC 1 cut(s) 215
MboII GAAGA 4 cut(s) 31, 128, 471, 550
MluCI AATT 4 cut(s) 119, 205, 273, 475
MnlI CCTC 7 cut(s) 168, 404, 427, 433, 436, 451, 523
MseI TTAA 1 cut(s) 204
MspI CCGG 1 cut(s) 52
MspR9I CCNGG 1 cut(s) 52
MvnI CGCG 1 cut(s) 379
MwoI GCNNNNNNNGC 1 cut(s) 52
NciI CCSGG 1 cut(s) 52
NlaIII CATG 4 cut(s) 46, 146, 254, 293
NlaIV GGNNCC 2 cut(s) 97, 127
NmuCI GTSAC 1 cut(s) 215
NspV TTCGAA 1 cut(s) 282
PaeR7I CTCGAG 1 cut(s) 463
PciSI GCTCTTC 1 cut(s) 141
PfeI GAWTC 1 cut(s) 89
PkrI GCNGC 5 cut(s) 48, 130, 133, 148, 244
PspN4I GGNNCC 2 cut(s) 97, 127
RsaI GTAC 1 cut(s) 109
RsaNI GTAC 1 cut(s) 108
SapI GCTCTTC 1 cut(s) 141
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 5 cut(s) 47, 129, 132, 147, 243
ScrFI CCNGG 1 cut(s) 52
SetI ASST 6 cut(s) 84, 115, 136, 179, 196, 460
SfaNI GCATC 1 cut(s) 253
Sfr274I CTCGAG 1 cut(s) 463
SfuI TTCGAA 1 cut(s) 282
SlaI CTCGAG 1 cut(s) 463
SmlI CTYRAG 1 cut(s) 463
SmoI CTYRAG 1 cut(s) 463
Sse9I AATT 4 cut(s) 119, 205, 273, 475
SsiI CCGC 3 cut(s) 129, 347, 379
SspMI CTAG 1 cut(s) 114
StyD4I CCNGG 1 cut(s) 50
TaaI ACNGT 3 cut(s) 25, 320, 521
TaqI TCGA 2 cut(s) 282, 464
TasI AATT 4 cut(s) 119, 205, 273, 475
TauI GCSGC 1 cut(s) 131
TfiI GAWTC 1 cut(s) 89
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TseFI GTSAC 1 cut(s) 215
TseI GCWGC 4 cut(s) 46, 131, 146, 242
Tsp45I GTSAC 1 cut(s) 215
TspDTI ATGAA 2 cut(s) 31, 128
XapI RAATTY 1 cut(s) 273
XhoI CTCGAG 1 cut(s) 463
XspI CTAG 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.