RLG00000021632

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002248
Physical Location & Seq
Reverse (-)
76894942 .. 76899861
4920 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021632

Sequence Viewer

Length: 1104 bp
ATGTGCAAGTACTTGCTGGAGGAATTGAAAATTGATGTTGATACCAAAGATGAAGATGGCGACACTCCCCTTATTCATGCTGCTCACCTAGGCCATACTGATACTGCCAAACTGCTTCTTGAGTATGGTGCCAATTCGTCTATAGCCAGTGATTTAGGGGCTACAGCTCTTCATCATTCCGCTGGACTAGGAAACATTGAGTTGCTGAGGTACTTAATTTCCAAGTGTGTTGATGTCAATTCACAAAGTGATGCTGGTTCTCCTTTAATTTGGGCTGCTGGTCATGACCAACTAGATGCTGTGAAAGTTCTGTTAGAACACCAGGCTAATCCTAATGTTGAAAGTGATGATGATATTACTCCATTGTTGTCATCTGTGGCAGCTGGTTCACTGGCATGCTTGGAGTTATTGATTCAGGCAGGTGCCAAAGTAAATATGAGTGCTGGTGGAGCGACCCCTTTACACATCGCTGCTGACATTGAGAACTTGGAGATTATAAAATGTTTGTTAAAAGCTGGAGCTGATCCCAGTGTAACTGATGAGACTTCGGGTTCGATAGGCGGTGTGTTAAGTGGTGGTGCAGCGCTTGGATTCTCGGAGGAGTTCTACAGAGGTGAACTTGAGGAGGCTGCTTGCAGCTGCTCCATAGCAACAAAACCATGCAAAACTTTCGCATTATGTTGCTACTTTATGAGAACAGCAGACAAGATCGAAGCCATTGCTTCGAAATTAGCTGATCCGTTGCCTAATTTGCAAGAACACCACGAGTCCCTTGCAAGAATTTTGGTCAAAGCAAAGTCTTCTGAAACAGGGGACAATCAGGATTCTTCGTCGCCTGTCAAGCTTGACGCCACAGCCCAAGTGCACATTGAAAAGCATAGAAGACTTCAGGAGGATTTGGCTGATGAAATGGTTCGATTGGCAAGGCAACTCAAAGAGACTAGTCTCATGATAAGCCACTCCGTGCAAAACACTGAAAAACTACTTGACTCTACAGAGGAGGCTGTTGAAAGGAGCTTAGCAAGCACTGGTCACGCGCCACTGCACGGGCATCCGATATATACTCAAAGACCTCCAAGACTTCGTGTTTCACATGGCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 Ank_5 DUF1685 TPR_17 TPR_8

Protein Analysis

368

Amino Acids

39.23

Weight (kDa)

5.11

Isoelectric Point (pI)

39.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 1 - 45 1.4e-07 Ankyrin repeats (3 copies)
Ank_4 PF13637 1 - 40 6.9e-06 Ankyrin repeats (many copies)
Ank PF00023 19 - 47 1.6e-06 Ankyrin repeat
Ank_4 PF13637 24 - 73 6.6e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 51 - 114 2e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 89 - 180 1.3e-15 Ankyrin repeats (3 copies)
Ank_4 PF13637 89 - 138 4.1e-06 Ankyrin repeats (many copies)
Ank_4 PF13637 128 - 170 2.9e-06 Ankyrin repeats (many copies)
Ank PF00023 150 - 180 2.5e-07 Ankyrin repeat
Use1 PF09753 265 - 346 4.5e-21 Membrane fusion protein Use1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 497
AarI CACCTGC 1 cut(s) 410
Acc36I ACCTGC 1 cut(s) 410
AccB1I GGYRCC 2 cut(s) 128, 422
AccII CGCG 1 cut(s) 1037
AciI CCGC 2 cut(s) 180, 561
AclWI GGATC 2 cut(s) 518, 731
AcsI RAATTY 1 cut(s) 780
AcuI CTGAAG 1 cut(s) 872
AcyI GRCGYC 1 cut(s) 849
AdeI CACNNNGTG 2 cut(s) 248, 964
AfaI GTAC 2 cut(s) 11, 212
AfeI AGCGCT 1 cut(s) 585
AfiI CCNNNNNNNGG 1 cut(s) 1046
AgsI TTSAA 4 cut(s) 28, 341, 872, 1010
AhlI ACTAGT 1 cut(s) 941
AjnI CCWGG 1 cut(s) 321
AluBI AGCT 8 cut(s) 167, 383, 515, 521, 639, 734, 844, 1017
AluI AGCT 8 cut(s) 167, 383, 515, 521, 639, 734, 844, 1017
Alw21I GWGCWC 1 cut(s) 867
Alw26I GTCTC 3 cut(s) 536, 932, 950
Alw44I GTGCAC 1 cut(s) 863
AlwI GGATC 2 cut(s) 518, 731
Aor51HI AGCGCT 1 cut(s) 585
AoxI GGCC 1 cut(s) 91
ApaLI GTGCAC 1 cut(s) 863
ApeKI GCWGC 8 cut(s) 80, 275, 380, 470, 581, 629, 636, 639
ApoI RAATTY 1 cut(s) 780
ArsI GACNNNNNNTTYG 2 cut(s) 535, 567
Asp700I GAANNNNTTC 1 cut(s) 912
AspA2I CCTAGG 1 cut(s) 88
AspLEI GCGC 2 cut(s) 586, 1039
AsuHPI GGTGA 2 cut(s) 77, 626
AsuII TTCGAA 1 cut(s) 725
AvrII CCTAGG 1 cut(s) 88
BaeGI GKGCMC 1 cut(s) 867
BanI GGYRCC 2 cut(s) 128, 422
BauI CACGAG 1 cut(s) 764
BbsI GAAGAC 2 cut(s) 792, 889
Bbv12I GWGCWC 1 cut(s) 867
BbvCI CCTCAGC 1 cut(s) 206
BbvI GCAGC 8 cut(s) 67, 262, 392, 457, 593, 616, 626, 648
BccI CCATC 1 cut(s) 50
BcgI CGANNNNNNTGC 6 cut(s) 652, 686, 701, 735, 755, 789
BciT130I CCWGG 1 cut(s) 323
BcoDI GTCTC 3 cut(s) 536, 932, 950
BcuI ACTAGT 1 cut(s) 941
BfaI CTAG 4 cut(s) 89, 188, 293, 942
BfmI CTRYAG 4 cut(s) 141, 162, 607, 993
BfoI RGCGCY 1 cut(s) 587
BfuAI ACCTGC 1 cut(s) 410
BisI GCNGC 8 cut(s) 81, 276, 381, 471, 582, 630, 637, 640
BlnI CCTAGG 1 cut(s) 88
BlpI GCTNAGC 1 cut(s) 1018
BlsI GCNGC 8 cut(s) 82, 277, 382, 472, 583, 631, 638, 641
BmcAI AGTACT 1 cut(s) 11
Bme1390I CCNGG 1 cut(s) 323
BmiI GGNNCC 2 cut(s) 130, 424
BmrFI CCNGG 1 cut(s) 323
BmrI ACTGGG 1 cut(s) 522
BmsI GCATC 3 cut(s) 241, 286, 1060
BmuI ACTGGG 1 cut(s) 522
BpiI GAAGAC 2 cut(s) 792, 889
BpmI CTGGAG 2 cut(s) 38, 537
Bpu10I CCTNAGC 1 cut(s) 206
Bpu1102I GCTNAGC 1 cut(s) 1018
Bpu14I TTCGAA 1 cut(s) 725
BpuEI CTTGAG 2 cut(s) 140, 641
BsaHI GRCGYC 1 cut(s) 849
BsaJI CCNNGG 1 cut(s) 88
Bsc4I CCNNNNNNNGG 1 cut(s) 1046
Bse1I ACTGG 4 cut(s) 147, 396, 528, 1033
Bse3DI GCAATG 1 cut(s) 717
BseBI CCWGG 1 cut(s) 323
BseDI CCNNGG 1 cut(s) 88
BseGI GGATG 1 cut(s) 1051
BseLI CCNNNNNNNGG 1 cut(s) 1046
BseMI GCAATG 1 cut(s) 717
BseMII CTCAG 1 cut(s) 197
BseNI ACTGG 4 cut(s) 147, 396, 528, 1033
BseRI GAGGAG 3 cut(s) 614, 638, 1013
BseSI GKGCMC 1 cut(s) 867
BseXI GCAGC 8 cut(s) 67, 262, 392, 457, 593, 616, 626, 648
BsgI GTGCAG 2 cut(s) 600, 1028
Bsh1236I CGCG 1 cut(s) 1037
BshFI GGCC 1 cut(s) 93
BshNI GGYRCC 2 cut(s) 128, 422
BsiHKAI GWGCWC 1 cut(s) 867
BslFI GGGAC 2 cut(s) 754, 827
BslI CCNNNNNNNGG 1 cut(s) 1046
BsmAI GTCTC 3 cut(s) 536, 932, 950
BsmFI GGGAC 2 cut(s) 754, 827
BsnI GGCC 1 cut(s) 93
Bsp119I TTCGAA 1 cut(s) 725
Bsp1286I GDGCHC 1 cut(s) 867
Bsp143I GATC 3 cut(s) 523, 708, 736
Bsp1720I GCTNAGC 1 cut(s) 1018
BspACI CCGC 2 cut(s) 180, 561
BspANI GGCC 1 cut(s) 93
BspCNI CTCAG 1 cut(s) 198
BspFNI CGCG 1 cut(s) 1037
BspHI TCATGA 2 cut(s) 283, 948
BspLI GGNNCC 2 cut(s) 130, 424
BspMI ACCTGC 1 cut(s) 410
BspPI GGATC 2 cut(s) 518, 731
BspQI GCTCTTC 1 cut(s) 174
BspT104I TTCGAA 1 cut(s) 725
BspT107I GGYRCC 2 cut(s) 128, 422
BsrDI GCAATG 1 cut(s) 717
BsrI ACTGG 4 cut(s) 147, 396, 528, 1033
BssECI CCNNGG 1 cut(s) 88
BssMI GATC 3 cut(s) 523, 708, 736
BssNI GRCGYC 1 cut(s) 849
BssSI CACGAG 1 cut(s) 764
BssT1I CCWWGG 1 cut(s) 88
Bst2BI CACGAG 1 cut(s) 764
Bst2UI CCWGG 1 cut(s) 323
Bst6I CTCTTC 1 cut(s) 174
BstACI GRCGYC 1 cut(s) 849
BstBI TTCGAA 1 cut(s) 725
BstC8I GCNNGC 3 cut(s) 397, 634, 1024
BstDEI CTNAG 2 cut(s) 206, 1018
BstF5I GGATG 1 cut(s) 1051
BstFNI CGCG 1 cut(s) 1037
BstH2I RGCGCY 1 cut(s) 587
BstHHI GCGC 2 cut(s) 586, 1039
BstKTI GATC 3 cut(s) 526, 711, 739
BstMAI GTCTC 3 cut(s) 536, 932, 950
BstMBI GATC 3 cut(s) 523, 708, 736
BstMWI GCNNNNNNNGC 4 cut(s) 449, 751, 841, 1023
BstNI CCWGG 1 cut(s) 323
BstNSI RCATGY 1 cut(s) 399
BstSCI CCNGG 1 cut(s) 321
BstSFI CTRYAG 4 cut(s) 141, 162, 607, 993
BstSLI GKGCMC 1 cut(s) 867
BstUI CGCG 1 cut(s) 1037
BstV1I GCAGC 8 cut(s) 67, 262, 392, 457, 593, 616, 626, 648
BstV2I GAAGAC 2 cut(s) 792, 889
BsuRI GGCC 1 cut(s) 93
BtgZI GCGATG 1 cut(s) 451
BtsCI GGATG 1 cut(s) 1051
BtsI GCAGTG 1 cut(s) 1040
BtsIMutI CAGTG 6 cut(s) 154, 389, 535, 972, 1026, 1040
BveI ACCTGC 1 cut(s) 410
Cac8I GCNNGC 3 cut(s) 397, 634, 1024
CciI TCATGA 2 cut(s) 283, 948
CfoI GCGC 2 cut(s) 586, 1039
CseI GACGC 1 cut(s) 857
Csp6I GTAC 2 cut(s) 10, 211
CviAII CATG 6 cut(s) 77, 284, 396, 660, 949, 1094
CviQI GTAC 2 cut(s) 10, 211
DdeI CTNAG 2 cut(s) 206, 1018
DpnI GATC 3 cut(s) 525, 710, 738
DpnII GATC 3 cut(s) 523, 708, 736
DraIII CACNNNGTG 2 cut(s) 248, 964
Eam1104I CTCTTC 1 cut(s) 174
EarI CTCTTC 1 cut(s) 174
Eco130I CCWWGG 1 cut(s) 88
Eco47III AGCGCT 1 cut(s) 585
Eco57I CTGAAG 1 cut(s) 872
EcoRII CCWGG 1 cut(s) 321
EcoT14I CCWWGG 1 cut(s) 88
ErhI CCWWGG 1 cut(s) 88
FaeI CATG 6 cut(s) 80, 287, 399, 663, 952, 1097
FaqI GGGAC 2 cut(s) 754, 827
FatI CATG 6 cut(s) 76, 283, 395, 659, 948, 1093
Fnu4HI GCNGC 8 cut(s) 81, 276, 381, 471, 582, 630, 637, 640
FokI GGATG 1 cut(s) 1038
Fsp4HI GCNGC 8 cut(s) 81, 276, 381, 471, 582, 630, 637, 640
FspBI CTAG 4 cut(s) 89, 188, 293, 942
GlaI GCGC 2 cut(s) 585, 1038
GluI GCNGC 8 cut(s) 81, 276, 381, 471, 582, 630, 637, 640
GsuI CTGGAG 2 cut(s) 38, 537
HaeII RGCGCY 1 cut(s) 587
HaeIII GGCC 1 cut(s) 93
HgaI GACGC 1 cut(s) 857
HhaI GCGC 2 cut(s) 586, 1039
Hin1I GRCGYC 1 cut(s) 849
Hin1II CATG 6 cut(s) 80, 287, 399, 663, 952, 1097
Hin6I GCGC 2 cut(s) 584, 1037
HinP1I GCGC 2 cut(s) 584, 1037
HindIII AAGCTT 1 cut(s) 842
HinfI GANTC 5 cut(s) 412, 591, 767, 824, 989
HphI GGTGA 2 cut(s) 77, 626
Hpy166II GTNNAC 3 cut(s) 389, 617, 865
Hpy188I TCNGA 3 cut(s) 598, 805, 1056
Hpy188III TCNNGA 5 cut(s) 119, 284, 821, 890, 949
Hpy8I GTNNAC 3 cut(s) 389, 617, 865
Hpy99I CGWCG 1 cut(s) 835
HpyCH4V TGCA 9 cut(s) 6, 581, 636, 663, 754, 776, 865, 967, 1045
HpyF10VI GCNNNNNNNGC 4 cut(s) 449, 751, 841, 1023
HpyF3I CTNAG 2 cut(s) 206, 1018
Hsp92I GRCGYC 1 cut(s) 849
Hsp92II CATG 6 cut(s) 80, 287, 399, 663, 952, 1097
HspAI GCGC 2 cut(s) 584, 1037
Kzo9I GATC 3 cut(s) 523, 708, 736
LguI GCTCTTC 1 cut(s) 174
LmnI GCTCC 4 cut(s) 449, 518, 647, 1014
Lsp1109I GCAGC 8 cut(s) 67, 262, 392, 457, 593, 616, 626, 648
LweI GCATC 3 cut(s) 241, 286, 1060
MaeI CTAG 4 cut(s) 89, 188, 293, 942
MaeIII GTNAC 2 cut(s) 532, 1031
MalI GATC 3 cut(s) 525, 710, 738
MboI GATC 3 cut(s) 523, 708, 736
MboII GAAGA 5 cut(s) 65, 161, 792, 819, 894
MhlI GDGCHC 1 cut(s) 867
MluCI AATT 9 cut(s) 23, 30, 133, 216, 238, 267, 728, 748, 780
MlyI GAGTC 2 cut(s) 776, 983
MroXI GAANNNNTTC 1 cut(s) 912
MseI TTAA 4 cut(s) 215, 266, 509, 569
MslI CAYNNNNRTG 1 cut(s) 394
MspA1I CMGCKG 3 cut(s) 182, 383, 639
MspR9I CCNGG 1 cut(s) 323
MvaI CCWGG 1 cut(s) 323
MvnI CGCG 1 cut(s) 1037
MwoI GCNNNNNNNGC 4 cut(s) 449, 751, 841, 1023
NdeII GATC 3 cut(s) 523, 708, 736
NlaIII CATG 6 cut(s) 80, 287, 399, 663, 952, 1097
NlaIV GGNNCC 2 cut(s) 130, 424
NmuCI GTSAC 1 cut(s) 1031
NspI RCATGY 1 cut(s) 399
NspV TTCGAA 1 cut(s) 725
PaeI GCATGC 1 cut(s) 399
PagI TCATGA 2 cut(s) 283, 948
PaqCI CACCTGC 1 cut(s) 410
PciSI GCTCTTC 1 cut(s) 174
PdmI GAANNNNTTC 1 cut(s) 912
PfeI GAWTC 3 cut(s) 412, 591, 824
PkrI GCNGC 8 cut(s) 82, 277, 382, 472, 583, 631, 638, 641
PleI GAGTC 2 cut(s) 775, 983
PpsI GAGTC 2 cut(s) 775, 983
PsiI TTATAA 1 cut(s) 497
Psp6I CCWGG 1 cut(s) 321
PspGI CCWGG 1 cut(s) 321
PspN4I GGNNCC 2 cut(s) 130, 424
PvuII CAGCTG 2 cut(s) 383, 639
RsaI GTAC 2 cut(s) 11, 212
RsaNI GTAC 2 cut(s) 10, 211
RseI CAYNNNNRTG 1 cut(s) 394
SapI GCTCTTC 1 cut(s) 174
SaqAI TTAA 4 cut(s) 215, 266, 509, 569
SatI GCNGC 8 cut(s) 81, 276, 381, 471, 582, 630, 637, 640
Sau3AI GATC 3 cut(s) 523, 708, 736
ScaI AGTACT 1 cut(s) 11
SchI GAGTC 2 cut(s) 776, 983
ScrFI CCNGG 1 cut(s) 323
SduI GDGCHC 1 cut(s) 867
SfaNI GCATC 3 cut(s) 241, 286, 1060
SfcI CTRYAG 4 cut(s) 141, 162, 607, 993
SfuI TTCGAA 1 cut(s) 725
SmiMI CAYNNNNRTG 1 cut(s) 394
SmlI CTYRAG 2 cut(s) 119, 620
SmoI CTYRAG 2 cut(s) 119, 620
SpeI ACTAGT 1 cut(s) 941
SphI GCATGC 1 cut(s) 399
Sse9I AATT 9 cut(s) 23, 30, 133, 216, 238, 267, 728, 748, 780
SsiI CCGC 2 cut(s) 180, 561
SspMI CTAG 4 cut(s) 89, 188, 293, 942
StyD4I CCNGG 1 cut(s) 321
StyI CCWWGG 1 cut(s) 88
TaqI TCGA 4 cut(s) 554, 711, 725, 916
TasI AATT 9 cut(s) 23, 30, 133, 216, 238, 267, 728, 748, 780
TatI WGTACW 1 cut(s) 9
TfiI GAWTC 3 cut(s) 412, 591, 824
Tru1I TTAA 4 cut(s) 215, 266, 509, 569
Tru9I TTAA 4 cut(s) 215, 266, 509, 569
TscAI CASTG 6 cut(s) 154, 396, 535, 979, 1033, 1047
TseFI GTSAC 1 cut(s) 1031
TseI GCWGC 8 cut(s) 80, 275, 380, 470, 581, 629, 636, 639
Tsp45I GTSAC 1 cut(s) 1031
TspDTI ATGAA 4 cut(s) 65, 66, 161, 921
TspGWI ACGGA 2 cut(s) 729, 952
TspRI CASTG 6 cut(s) 154, 396, 535, 979, 1033, 1047
VneI GTGCAC 1 cut(s) 863
XapI RAATTY 1 cut(s) 780
XceI RCATGY 1 cut(s) 399
XmaJI CCTAGG 1 cut(s) 88
XmnI GAANNNNTTC 1 cut(s) 912
XspI CTAG 4 cut(s) 89, 188, 293, 942
ZrmI AGTACT 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.