Rw6G017710

ankyrin repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0002248
Physical Location & Seq
Forward (+)
35034533 .. 35037697
3165 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G017710.1

Sequence Viewer

Length: 567 bp
ATGTATTTAGTGTCTGCAGCTGCATTTAAGTTGGCGGAGAAACTTGATGAAGGGAAGGGATTGGCCAAAACTGTGGCTGATATTAAGGATGCCAACAAGCGTGGCGCACTTCATTTTGCTGCAAGAGAGGGCCAGACTGAGATGTGCAAGTACTTGCGGGAGGAATTGAAAATTGATGTTGATACCAAAGATAAAGATGACGACACTCCCCTTATTCATGTTGCTCGCCTAGGCCATACTGATACTGCCAAACTGCTTCTTGAGTATGGTGCCAATCCATCTAGAGCCAGTGATTTAGGGGCTACAGCTTTTCATCATTCAGCTGGACTAGGAAACATTGACTTGCTGAGGTACTTAATTTCCAAGGGTGTCGATGTCAATTCACAAAGTGATGATGTTTGCTTGGTGGTTCTGTACAGGGAGTACCTATCTTGTGCCAAAGTAAATATGAGTACTGGTGGAGCGACCCCTTTACACATCGCTGCTGATATTGGGAACTTGGAGATTATAAAATGTTTGTTAAAAGTTGGAGCTGATCCCAGTGTAACTAATAAGGTGAGGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 Ank_5 DUF1685 TPR_17 TPR_8

Protein Analysis

188

Amino Acids

20.31

Weight (kDa)

6.3

Isoelectric Point (pI)

18.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 24 - 94 2.6e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 37 - 84 3.4e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 58 - 129 3e-13 Ankyrin repeats (3 copies)
Ank_2 PF12796 100 - 184 5.5e-10 Ankyrin repeats (3 copies)
Ank_4 PF13637 110 - 174 3.4e-07 Ankyrin repeats (many copies)
Ank PF00023 154 - 185 3.4e-08 Ankyrin repeat
Ank_3 PF13606 154 - 181 4.1e-06 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 509
AccB1I GGYRCC 1 cut(s) 269
AciI CCGC 2 cut(s) 35, 157
AclWI GGATC 1 cut(s) 530
AcoI YGGCCR 1 cut(s) 63
AdeI CACNNNGTG 1 cut(s) 389
AfaI GTAC 5 cut(s) 152, 353, 416, 425, 454
AgsI TTSAA 1 cut(s) 169
AluBI AGCT 4 cut(s) 20, 308, 323, 533
AluI AGCT 4 cut(s) 20, 308, 323, 533
AlwI GGATC 1 cut(s) 530
AoxI GGCC 3 cut(s) 63, 130, 232
ApeKI GCWGC 4 cut(s) 17, 20, 119, 482
AspA2I CCTAGG 1 cut(s) 229
AspLEI GCGC 1 cut(s) 107
AspS9I GGNCC 1 cut(s) 130
AvrII CCTAGG 1 cut(s) 229
BalI TGGCCA 1 cut(s) 65
BanI GGYRCC 1 cut(s) 269
BbvCI CCTCAGC 1 cut(s) 347
BbvI GCAGC 4 cut(s) 7, 29, 106, 469
BccI CCATC 1 cut(s) 286
BfaI CTAG 3 cut(s) 230, 282, 329
BfmI CTRYAG 2 cut(s) 15, 303
BisI GCNGC 4 cut(s) 18, 21, 120, 483
BlnI CCTAGG 1 cut(s) 229
BlsI GCNGC 4 cut(s) 19, 22, 121, 484
BmcAI AGTACT 2 cut(s) 152, 454
BmgT120I GGNCC 1 cut(s) 130
BmiI GGNNCC 1 cut(s) 271
BmrI ACTGGG 1 cut(s) 534
BmsI GCATC 1 cut(s) 79
BmuI ACTGGG 1 cut(s) 534
Bpu10I CCTNAGC 1 cut(s) 347
BpuEI CTTGAG 1 cut(s) 281
BsaJI CCNNGG 2 cut(s) 229, 363
Bse1I ACTGG 3 cut(s) 288, 460, 540
BseDI CCNNGG 2 cut(s) 229, 363
BseGI GGATG 1 cut(s) 94
BseMII CTCAG 2 cut(s) 129, 338
BseNI ACTGG 3 cut(s) 288, 460, 540
BseXI GCAGC 4 cut(s) 7, 29, 106, 469
BshFI GGCC 3 cut(s) 65, 132, 234
BshNI GGYRCC 1 cut(s) 269
BsnI GGCC 3 cut(s) 65, 132, 234
Bsp1407I TGTACA 1 cut(s) 414
Bsp143I GATC 1 cut(s) 535
BspACI CCGC 2 cut(s) 35, 157
BspANI GGCC 3 cut(s) 65, 132, 234
BspCNI CTCAG 2 cut(s) 130, 339
BspLI GGNNCC 1 cut(s) 271
BspMAI CTGCAG 1 cut(s) 19
BspPI GGATC 1 cut(s) 530
BspT107I GGYRCC 1 cut(s) 269
BsrGI TGTACA 1 cut(s) 414
BsrI ACTGG 3 cut(s) 288, 460, 540
BssECI CCNNGG 2 cut(s) 229, 363
BssMI GATC 1 cut(s) 535
BssT1I CCWWGG 2 cut(s) 229, 363
Bst4CI ACNGT 1 cut(s) 73
BstAUI TGTACA 1 cut(s) 414
BstC8I GCNNGC 1 cut(s) 226
BstDEI CTNAG 2 cut(s) 138, 347
BstF5I GGATG 1 cut(s) 94
BstHHI GCGC 1 cut(s) 107
BstKTI GATC 1 cut(s) 538
BstMBI GATC 1 cut(s) 535
BstSFI CTRYAG 2 cut(s) 15, 303
BstV1I GCAGC 4 cut(s) 7, 29, 106, 469
BstXI CCANNNNNNTGG 1 cut(s) 73
BsuRI GGCC 3 cut(s) 65, 132, 234
BtgZI GCGATG 1 cut(s) 463
BtsCI GGATG 1 cut(s) 94
BtsIMutI CAGTG 2 cut(s) 295, 547
Cac8I GCNNGC 1 cut(s) 226
CfoI GCGC 1 cut(s) 107
Cfr13I GGNCC 1 cut(s) 130
Csp6I GTAC 5 cut(s) 151, 352, 415, 424, 453
CspCI CAANNNNNGTGG 2 cut(s) 82, 117
CviAII CATG 1 cut(s) 218
CviQI GTAC 5 cut(s) 151, 352, 415, 424, 453
DdeI CTNAG 2 cut(s) 138, 347
DpnI GATC 1 cut(s) 537
DpnII GATC 1 cut(s) 535
DraIII CACNNNGTG 1 cut(s) 389
EaeI YGGCCR 1 cut(s) 63
EciI GGCGGA 1 cut(s) 50
Eco130I CCWWGG 2 cut(s) 229, 363
EcoT14I CCWWGG 2 cut(s) 229, 363
ErhI CCWWGG 2 cut(s) 229, 363
FaeI CATG 1 cut(s) 221
FaiI YATR 6 cut(s) 219, 237, 267, 449, 509, 565
FatI CATG 1 cut(s) 217
FauI CCCGC 1 cut(s) 150
Fnu4HI GCNGC 4 cut(s) 18, 21, 120, 483
FokI GGATG 1 cut(s) 101
Fsp4HI GCNGC 4 cut(s) 18, 21, 120, 483
FspBI CTAG 3 cut(s) 230, 282, 329
GlaI GCGC 1 cut(s) 106
GluI GCNGC 4 cut(s) 18, 21, 120, 483
HaeIII GGCC 3 cut(s) 65, 132, 234
HhaI GCGC 1 cut(s) 107
Hin1II CATG 1 cut(s) 221
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
Hpy188III TCNNGA 2 cut(s) 260, 282
HpyAV CCTTC 2 cut(s) 44, 49
HpyCH4III ACNGT 1 cut(s) 73
HpyCH4V TGCA 4 cut(s) 17, 23, 122, 147
HpyF3I CTNAG 2 cut(s) 138, 347
Hsp92II CATG 1 cut(s) 221
HspAI GCGC 1 cut(s) 105
Kzo9I GATC 1 cut(s) 535
LmnI GCTCC 2 cut(s) 461, 530
LpnPI CCDG 6 cut(s) 146, 301, 309, 403, 441, 553
Lsp1109I GCAGC 4 cut(s) 7, 29, 106, 469
LweI GCATC 1 cut(s) 79
MaeI CTAG 3 cut(s) 230, 282, 329
MaeIII GTNAC 1 cut(s) 544
MalI GATC 1 cut(s) 537
MboI GATC 1 cut(s) 535
MlsI TGGCCA 1 cut(s) 65
MluCI AATT 4 cut(s) 164, 171, 357, 379
MluNI TGGCCA 1 cut(s) 65
MmeI TCCRAC 1 cut(s) 508
MnlI CCTC 4 cut(s) 121, 154, 342, 552
Mox20I TGGCCA 1 cut(s) 65
MscI TGGCCA 1 cut(s) 65
MseI TTAA 4 cut(s) 27, 84, 356, 521
Msp20I TGGCCA 1 cut(s) 65
MspA1I CMGCKG 2 cut(s) 20, 323
NdeII GATC 1 cut(s) 535
NlaIII CATG 1 cut(s) 221
NlaIV GGNNCC 1 cut(s) 271
PkrI GCNGC 4 cut(s) 19, 22, 121, 484
PsiI TTATAA 1 cut(s) 509
PspN4I GGNNCC 1 cut(s) 271
PspPI GGNCC 1 cut(s) 130
PstI CTGCAG 1 cut(s) 19
PvuII CAGCTG 2 cut(s) 20, 323
RsaI GTAC 5 cut(s) 152, 353, 416, 425, 454
RsaNI GTAC 5 cut(s) 151, 352, 415, 424, 453
SaqAI TTAA 4 cut(s) 27, 84, 356, 521
SatI GCNGC 4 cut(s) 18, 21, 120, 483
Sau3AI GATC 1 cut(s) 535
Sau96I GGNCC 1 cut(s) 130
ScaI AGTACT 2 cut(s) 152, 454
SetI ASST 7 cut(s) 22, 310, 325, 353, 429, 535, 558
SfaNI GCATC 1 cut(s) 79
SfcI CTRYAG 2 cut(s) 15, 303
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
Sse9I AATT 4 cut(s) 164, 171, 357, 379
SsiI CCGC 2 cut(s) 35, 157
SspMI CTAG 3 cut(s) 230, 282, 329
StyI CCWWGG 2 cut(s) 229, 363
TaaI ACNGT 1 cut(s) 73
TaqI TCGA 1 cut(s) 372
TasI AATT 4 cut(s) 164, 171, 357, 379
TatI WGTACW 3 cut(s) 150, 414, 452
Tru1I TTAA 4 cut(s) 27, 84, 356, 521
Tru9I TTAA 4 cut(s) 27, 84, 356, 521
TscAI CASTG 2 cut(s) 295, 547
TseI GCWGC 4 cut(s) 17, 20, 119, 482
TspDTI ATGAA 4 cut(s) 63, 101, 206, 302
TspRI CASTG 2 cut(s) 295, 547
XbaI TCTAGA 1 cut(s) 281
XmaJI CCTAGG 1 cut(s) 229
XspI CTAG 3 cut(s) 230, 282, 329
ZrmI AGTACT 2 cut(s) 152, 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.