FvH4_6g02680

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002248
Physical Location & Seq
Forward (+)
1532630 .. 1537176
4547 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g02680.t1

Sequence Viewer

Length: 1254 bp
ATGGCTCCGGACGCTTCTGAAGCACTTGCAGCTAGGGAAATAGTTCAGCAGTTCCTGAATGCTGCATGTACTGGGAATGTTGATCTCTTAAAAAAGTTGGCTGGGAAACTCGATGAAGGGAAGGGTTTACCCAAAACTGTGGCTGATATTAAGGATGCCAACAAGCGTGGCGCACTTCATTTTGCTGCAAGAGAGGGCCAGACTGAGATGTGCAAGTACTTGCTGGAGGAATTGAAACTTGATGTTGATACCAAAGATGAAGATGGCGACACTCCCCTTATTCATGCTGCTCGTCTTGGCCATACTGATACTGCAAAACTTCTTCTTGAGTATGGTGCCAATCCATCTATCGCCAGTGATTTAGGGGCTACGGCTCTTCATCATTCTGCTGGACTAGGAAACATTGAGTTGCTGAGGTGCTTAATTTCCAAGGGTGTCGATATCAATTCACAAAGTGATGCGGGTTCTCCTTTAATTTGGGCTGCTGGTCATGACCAACCAGATGCTGTGAAAATTCTGTTAGAACACCAAGCTAATCCTAATGCTGAAAGTGATGATGATATTACTCCATTGTTGTCAGCTGTGGCAGCTGGTTCACTGGCATGCTTAGAGTTATTGATTCAGGCAGGTGCCAAAGTAAATATGAGTGCTGGTGGAGCGACCCCTTTACACATAGCTGCTGATATTGGGAGCTTGGAGATTATAAAATGTTTATTAAAAGCCGGAGCAGATCCCAATGTAACTGATGAGGAGGGTTTGAAGCCAATACAGATTGCAGCTGCAAGTGGGAACCGAAGTGTTGTGGAGGTCCTCTTTCCCTTGACATCACAAGTTGAAACCATTTCAAAATGGACAGTTGATGGCATACTTGAGTATATGCAATCTGAAACAAGCAAACAACAGGAACAATCGCGAAATACCAAGGAGCTTAATGTCCCAAAAGACTCTACTTCACTAAAGAAAGAGCTTCCTGAGGTGACACCTGAGGCAAAGAAAAGAGCCGCAGAAGCAAAATCAAGAGGAGGTGATGCTTTCAGTAGGAAGGATTATCAAGCGGCTGTTGATGCATATACCCAGGCAATTGATATGGACCCAACTGATCCCATTTTTCTATCCAATCGAAGCCTTTGTTGGCTTCGTCTTGGCCAACCTGAGCATGCCTTAGCCGATGCAAAGGCCTGCAGGGAATTGAAACCAGATTGGCCAAAAGCTTGCTTTCGAGAAGGTGCAGCATTGCGCCTAATGCAGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 Ank_5 DUF1685 TPR_17 TPR_8

Protein Analysis

418

Amino Acids

44.55

Weight (kDa)

5.09

Isoelectric Point (pI)

34.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 20 - 116 2.2e-17 Ankyrin repeats (3 copies)
Ank_4 PF13637 59 - 101 2e-07 Ankyrin repeats (many copies)
Ank PF00023 88 - 118 2e-07 Ankyrin repeat
Ank_4 PF13637 94 - 142 2.3e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 114 - 183 2e-11 Ankyrin repeats (3 copies)
Ank_4 PF13637 158 - 207 1.8e-06 Ankyrin repeats (many copies)
Ank_4 PF13637 191 - 239 2e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 192 - 273 2.4e-13 Ankyrin repeats (3 copies)
Ank_5 PF13857 206 - 259 6.4e-09 Ankyrin repeats (many copies)
Ank PF00023 219 - 249 1.2e-08 Ankyrin repeat
Ank_3 PF13606 219 - 247 8.1e-06 Ankyrin repeat
Ank_4 PF13637 230 - 271 1.6e-06 Ankyrin repeats (many copies)
TPR_BSK1_C PF25575 330 - 406 1.8e-14 Serine/threonine-protein kinase BSK1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 704
AarI CACCTGC 1 cut(s) 617
Acc36I ACCTGC 1 cut(s) 617
AccB1I GGYRCC 2 cut(s) 335, 629
AccII CGCG 1 cut(s) 913
AccIII TCCGGA 1 cut(s) 7
AciI CCGC 3 cut(s) 461, 1002, 1055
AclWI GGATC 2 cut(s) 725, 1094
AcoI YGGCCR 3 cut(s) 298, 1144, 1202
AcsI RAATTY 1 cut(s) 513
AcuI CTGAAG 1 cut(s) 39
AdeI CACNNNGTG 1 cut(s) 455
AfaI GTAC 2 cut(s) 70, 218
AfiI CCNNNNNNNGG 1 cut(s) 1246
AgsI TTSAA 5 cut(s) 235, 760, 836, 846, 1192
AjnI CCWGG 1 cut(s) 1074
AjuI GAANNNNNNNTTGG 2 cut(s) 1114, 1146
AlwI GGATC 2 cut(s) 725, 1094
AlwNI CAGNNNCTG 2 cut(s) 55, 506
Aor13HI TCCGGA 1 cut(s) 7
AoxI GGCC 5 cut(s) 196, 298, 1144, 1176, 1202
ApoI RAATTY 1 cut(s) 513
Asp700I GAANNNNTTC 1 cut(s) 42
AspLEI GCGC 2 cut(s) 173, 1239
AspS9I GGNCC 3 cut(s) 196, 808, 1090
AsuHPI GGTGA 2 cut(s) 988, 1037
AvaII GGWCC 2 cut(s) 808, 1090
AxyI CCTNAGG 2 cut(s) 972, 984
BalI TGGCCA 3 cut(s) 300, 1146, 1204
BanI GGYRCC 2 cut(s) 335, 629
BbvCI CCTCAGC 1 cut(s) 413
BccI CCATC 3 cut(s) 257, 352, 854
BceAI ACGGC 1 cut(s) 387
BciT130I CCWGG 1 cut(s) 1076
BfaI CTAG 2 cut(s) 33, 395
BfmI CTRYAG 1 cut(s) 1180
BfuAI ACCTGC 1 cut(s) 617
BmcAI AGTACT 1 cut(s) 218
Bme1390I CCNGG 1 cut(s) 1076
Bme18I GGWCC 2 cut(s) 808, 1090
BmgT120I GGNCC 3 cut(s) 196, 808, 1090
BmiI GGNNCC 5 cut(s) 6, 337, 631, 791, 1092
BmrFI CCNGG 1 cut(s) 1076
BmrI ACTGGG 1 cut(s) 81
BmsI GCATC 6 cut(s) 145, 448, 493, 1018, 1054, 1159
BmuI ACTGGG 1 cut(s) 81
BpmI CTGGAG 1 cut(s) 245
Bpu10I CCTNAGC 3 cut(s) 413, 1152, 1162
BpuEI CTTGAG 2 cut(s) 347, 890
BsaJI CCNNGG 3 cut(s) 429, 921, 1074
BsaWI WCCGGW 1 cut(s) 7
Bsc4I CCNNNNNNNGG 1 cut(s) 1246
Bse1I ACTGG 3 cut(s) 76, 354, 603
Bse21I CCTNAGG 2 cut(s) 972, 984
Bse3DI GCAATG 1 cut(s) 1232
BseAI TCCGGA 1 cut(s) 7
BseBI CCWGG 1 cut(s) 1076
BseDI CCNNGG 3 cut(s) 429, 921, 1074
BseGI GGATG 1 cut(s) 160
BseLI CCNNNNNNNGG 1 cut(s) 1246
BseMI GCAATG 1 cut(s) 1232
BseMII CTCAG 5 cut(s) 195, 404, 963, 975, 1143
BseNI ACTGG 3 cut(s) 76, 354, 603
BseRI GAGGAG 2 cut(s) 764, 1035
BseYI CCCAGC 1 cut(s) 101
BsgI GTGCAG 1 cut(s) 1248
Bsh1236I CGCG 1 cut(s) 913
BshFI GGCC 5 cut(s) 198, 300, 1146, 1178, 1204
BshNI GGYRCC 2 cut(s) 335, 629
BsiSI CCGG 2 cut(s) 8, 723
BslFI GGGAC 1 cut(s) 920
BslI CCNNNNNNNGG 1 cut(s) 1246
BsmFI GGGAC 1 cut(s) 920
BsmI GAATGC 1 cut(s) 64
BsnI GGCC 5 cut(s) 198, 300, 1146, 1178, 1204
Bsp13I TCCGGA 1 cut(s) 7
Bsp143I GATC 3 cut(s) 82, 730, 1099
Bsp68I TCGCGA 1 cut(s) 913
BspACI CCGC 3 cut(s) 461, 1002, 1055
BspANI GGCC 5 cut(s) 198, 300, 1146, 1178, 1204
BspCNI CTCAG 5 cut(s) 196, 405, 964, 976, 1144
BspEI TCCGGA 1 cut(s) 7
BspFNI CGCG 1 cut(s) 913
BspHI TCATGA 1 cut(s) 490
BspLI GGNNCC 5 cut(s) 6, 337, 631, 791, 1092
BspMAI CTGCAG 1 cut(s) 1184
BspMI ACCTGC 1 cut(s) 617
BspPI GGATC 2 cut(s) 725, 1094
BspQI GCTCTTC 1 cut(s) 381
BspT107I GGYRCC 2 cut(s) 335, 629
BsrDI GCAATG 1 cut(s) 1232
BsrI ACTGG 3 cut(s) 76, 354, 603
BssECI CCNNGG 3 cut(s) 429, 921, 1074
BssMI GATC 3 cut(s) 82, 730, 1099
BssT1I CCWWGG 2 cut(s) 429, 921
Bst2UI CCWGG 1 cut(s) 1076
Bst4CI ACNGT 2 cut(s) 139, 856
Bst6I CTCTTC 1 cut(s) 381
BstC8I GCNNGC 5 cut(s) 604, 1158, 1180, 1213, 1248
BstDEI CTNAG 7 cut(s) 204, 413, 607, 972, 984, 1152, 1162
BstENI CCTNNNNNAGG 1 cut(s) 1244
BstF5I GGATG 1 cut(s) 160
BstFNI CGCG 1 cut(s) 913
BstHHI GCGC 2 cut(s) 173, 1239
BstKTI GATC 3 cut(s) 85, 733, 1102
BstMBI GATC 3 cut(s) 82, 730, 1099
BstMWI GCNNNNNNNGC 8 cut(s) 11, 20, 29, 587, 656, 1007, 1064, 1243
BstNI CCWGG 1 cut(s) 1076
BstNSI RCATGY 3 cut(s) 69, 606, 1160
BstSCI CCNGG 1 cut(s) 1074
BstSFI CTRYAG 1 cut(s) 1180
BstUI CGCG 1 cut(s) 913
BstX2I RGATCY 1 cut(s) 730
BstXI CCANNNNNNTGG 1 cut(s) 139
BstYI RGATCY 1 cut(s) 730
Bsu36I CCTNAGG 2 cut(s) 972, 984
BsuRI GGCC 5 cut(s) 198, 300, 1146, 1178, 1204
BtsCI GGATG 1 cut(s) 160
BtsIMutI CAGTG 2 cut(s) 361, 596
BtuMI TCGCGA 1 cut(s) 913
BveI ACCTGC 1 cut(s) 617
Cac8I GCNNGC 5 cut(s) 604, 1158, 1180, 1213, 1248
CaiI CAGNNNCTG 2 cut(s) 55, 506
CciI TCATGA 1 cut(s) 490
CfoI GCGC 2 cut(s) 173, 1239
Cfr13I GGNCC 3 cut(s) 196, 808, 1090
CseI GACGC 1 cut(s) 20
Csp6I GTAC 2 cut(s) 69, 217
CspCI CAANNNNNGTGG 2 cut(s) 148, 183
CviAII CATG 5 cut(s) 66, 284, 491, 603, 1157
CviQI GTAC 2 cut(s) 69, 217
DdeI CTNAG 7 cut(s) 204, 413, 607, 972, 984, 1152, 1162
DpnI GATC 3 cut(s) 84, 732, 1101
DpnII GATC 3 cut(s) 82, 730, 1099
DraIII CACNNNGTG 1 cut(s) 455
EaeI YGGCCR 3 cut(s) 298, 1144, 1202
Eam1104I CTCTTC 1 cut(s) 381
EarI CTCTTC 1 cut(s) 381
Eco130I CCWWGG 2 cut(s) 429, 921
Eco147I AGGCCT 1 cut(s) 1178
Eco32I GATATC 1 cut(s) 442
Eco47I GGWCC 2 cut(s) 808, 1090
Eco57I CTGAAG 1 cut(s) 39
Eco81I CCTNAGG 2 cut(s) 972, 984
EcoNI CCTNNNNNAGG 1 cut(s) 1244
EcoO109I RGGNCCY 1 cut(s) 808
EcoRII CCWGG 1 cut(s) 1074
EcoRV GATATC 1 cut(s) 442
EcoT14I CCWWGG 2 cut(s) 429, 921
EcoT22I ATGCAT 1 cut(s) 1069
ErhI CCWWGG 2 cut(s) 429, 921
FaeI CATG 5 cut(s) 69, 287, 494, 606, 1160
FaqI GGGAC 1 cut(s) 920
FatI CATG 5 cut(s) 65, 283, 490, 602, 1156
FauI CCCGC 1 cut(s) 454
FokI GGATG 1 cut(s) 167
FspBI CTAG 2 cut(s) 33, 395
GlaI GCGC 2 cut(s) 172, 1238
GsaI CCCAGC 1 cut(s) 105
GsuI CTGGAG 1 cut(s) 245
HaeIII GGCC 5 cut(s) 198, 300, 1146, 1178, 1204
HapII CCGG 2 cut(s) 8, 723
HgaI GACGC 1 cut(s) 20
HhaI GCGC 2 cut(s) 173, 1239
Hin1II CATG 5 cut(s) 69, 287, 494, 606, 1160
Hin6I GCGC 2 cut(s) 171, 1237
HinP1I GCGC 2 cut(s) 171, 1237
HindIII AAGCTT 1 cut(s) 1209
HinfI GANTC 2 cut(s) 619, 944
HpaII CCGG 2 cut(s) 8, 723
HphI GGTGA 2 cut(s) 988, 1037
Hpy166II GTNNAC 2 cut(s) 128, 596
Hpy188I TCNGA 2 cut(s) 19, 886
Hpy188III TCNNGA 8 cut(s) 8, 55, 326, 491, 912, 971, 1017, 1220
Hpy8I GTNNAC 2 cut(s) 128, 596
HpyAV CCTTC 4 cut(s) 110, 115, 1036, 1217
HpyCH4III ACNGT 2 cut(s) 139, 856
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 20, 29, 587, 656, 1007, 1064, 1243
HpyF3I CTNAG 7 cut(s) 204, 413, 607, 972, 984, 1152, 1162
Hsp92II CATG 5 cut(s) 69, 287, 494, 606, 1160
HspAI GCGC 2 cut(s) 171, 1237
Kpn2I TCCGGA 1 cut(s) 7
Kzo9I GATC 3 cut(s) 82, 730, 1099
LguI GCTCTTC 1 cut(s) 381
LmnI GCTCC 5 cut(s) 10, 656, 690, 725, 925
LweI GCATC 6 cut(s) 145, 448, 493, 1018, 1054, 1159
MaeI CTAG 2 cut(s) 33, 395
MaeIII GTNAC 2 cut(s) 739, 976
MalI GATC 3 cut(s) 84, 732, 1101
MboI GATC 3 cut(s) 82, 730, 1099
MboII GAAGA 3 cut(s) 272, 314, 368
MfeI CAATTG 1 cut(s) 1080
MflI RGATCY 1 cut(s) 730
MlsI TGGCCA 3 cut(s) 300, 1146, 1204
MluCI AATT 7 cut(s) 230, 423, 445, 474, 513, 1080, 1187
MluNI TGGCCA 3 cut(s) 300, 1146, 1204
MlyI GAGTC 1 cut(s) 938
Mox20I TGGCCA 3 cut(s) 300, 1146, 1204
Mph1103I ATGCAT 1 cut(s) 1069
MroI TCCGGA 1 cut(s) 7
MroXI GAANNNNTTC 1 cut(s) 42
MscI TGGCCA 3 cut(s) 300, 1146, 1204
MseI TTAA 6 cut(s) 89, 150, 422, 473, 716, 930
MslI CAYNNNNRTG 1 cut(s) 601
Msp20I TGGCCA 3 cut(s) 300, 1146, 1204
MspA1I CMGCKG 3 cut(s) 581, 590, 779
MspI CCGG 2 cut(s) 8, 723
MspR9I CCNGG 1 cut(s) 1076
MunI CAATTG 1 cut(s) 1080
Mva1269I GAATGC 1 cut(s) 64
MvaI CCWGG 1 cut(s) 1076
MvnI CGCG 1 cut(s) 913
MwoI GCNNNNNNNGC 8 cut(s) 11, 20, 29, 587, 656, 1007, 1064, 1243
NdeII GATC 3 cut(s) 82, 730, 1099
NlaIII CATG 5 cut(s) 69, 287, 494, 606, 1160
NlaIV GGNNCC 5 cut(s) 6, 337, 631, 791, 1092
NmuCI GTSAC 1 cut(s) 976
NruI TCGCGA 1 cut(s) 913
NsiI ATGCAT 1 cut(s) 1069
NspI RCATGY 3 cut(s) 69, 606, 1160
PaeI GCATGC 2 cut(s) 606, 1160
PagI TCATGA 1 cut(s) 490
PaqCI CACCTGC 1 cut(s) 617
PceI AGGCCT 1 cut(s) 1178
PciSI GCTCTTC 1 cut(s) 381
PctI GAATGC 1 cut(s) 64
PdmI GAANNNNTTC 1 cut(s) 42
PfeI GAWTC 1 cut(s) 619
PleI GAGTC 1 cut(s) 938
PpsI GAGTC 1 cut(s) 938
PpuMI RGGWCCY 1 cut(s) 808
PsiI TTATAA 1 cut(s) 704
Psp5II RGGWCCY 1 cut(s) 808
Psp6I CCWGG 1 cut(s) 1074
PspFI CCCAGC 1 cut(s) 101
PspGI CCWGG 1 cut(s) 1074
PspN4I GGNNCC 5 cut(s) 6, 337, 631, 791, 1092
PspPI GGNCC 3 cut(s) 196, 808, 1090
PspPPI RGGWCCY 1 cut(s) 808
PstI CTGCAG 1 cut(s) 1184
PstNI CAGNNNCTG 2 cut(s) 55, 506
PsuI RGATCY 1 cut(s) 730
PvuII CAGCTG 3 cut(s) 581, 590, 779
RruI TCGCGA 1 cut(s) 913
RsaI GTAC 2 cut(s) 70, 218
RsaNI GTAC 2 cut(s) 69, 217
RseI CAYNNNNRTG 1 cut(s) 601
SapI GCTCTTC 1 cut(s) 381
SaqAI TTAA 6 cut(s) 89, 150, 422, 473, 716, 930
Sau3AI GATC 3 cut(s) 82, 730, 1099
Sau96I GGNCC 3 cut(s) 196, 808, 1090
SbfI CCTGCAGG 1 cut(s) 1184
ScaI AGTACT 1 cut(s) 218
SchI GAGTC 1 cut(s) 938
ScrFI CCNGG 1 cut(s) 1076
SdaI CCTGCAGG 1 cut(s) 1184
SfaNI GCATC 6 cut(s) 145, 448, 493, 1018, 1054, 1159
SfcI CTRYAG 1 cut(s) 1180
SinI GGWCC 2 cut(s) 808, 1090
SmiMI CAYNNNNRTG 1 cut(s) 601
SmlI CTYRAG 2 cut(s) 326, 869
SmoI CTYRAG 2 cut(s) 326, 869
SphI GCATGC 2 cut(s) 606, 1160
Sse8387I CCTGCAGG 1 cut(s) 1184
Sse9I AATT 7 cut(s) 230, 423, 445, 474, 513, 1080, 1187
SseBI AGGCCT 1 cut(s) 1178
SsiI CCGC 3 cut(s) 461, 1002, 1055
SspMI CTAG 2 cut(s) 33, 395
StuI AGGCCT 1 cut(s) 1178
StyD4I CCNGG 1 cut(s) 1074
StyI CCWWGG 2 cut(s) 429, 921
TaaI ACNGT 2 cut(s) 139, 856
TaqI TCGA 4 cut(s) 111, 438, 1120, 1219
TasI AATT 7 cut(s) 230, 423, 445, 474, 513, 1080, 1187
TatI WGTACW 2 cut(s) 68, 216
TauI GCSGC 2 cut(s) 1004, 1058
TfiI GAWTC 1 cut(s) 619
Tru1I TTAA 6 cut(s) 89, 150, 422, 473, 716, 930
Tru9I TTAA 6 cut(s) 89, 150, 422, 473, 716, 930
TscAI CASTG 2 cut(s) 361, 603
TseFI GTSAC 1 cut(s) 976
Tsp45I GTSAC 1 cut(s) 976
TspDTI ATGAA 5 cut(s) 129, 167, 272, 273, 368
TspRI CASTG 2 cut(s) 361, 603
VpaK11BI GGWCC 2 cut(s) 808, 1090
XagI CCTNNNNNAGG 1 cut(s) 1244
XapI RAATTY 1 cut(s) 513
XceI RCATGY 3 cut(s) 69, 606, 1160
XmnI GAANNNNTTC 1 cut(s) 42
XspI CTAG 2 cut(s) 33, 395
ZrmI AGTACT 1 cut(s) 218
Zsp2I ATGCAT 1 cut(s) 1069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.