RLG00000033656

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002248
Physical Location & Seq
Forward (+)
30273461 .. 30276786
3326 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033656

Sequence Viewer

Length: 633 bp
ATGGTGATCTATGATAGCCGTCGGATCAGTGAAGAGCTGATCGAACGGTCAATTGACACAAGAGAGGGCCAGACTGAGATGTGCAAGTACTTGCTGGAGGAATTGAAAATTGATGTTGATACCAAAGATGAAGATGGCGACACTCCCCTTATTCATGCTGCTCACCTAGGCCATACTGATACTGCCAAACTGCTTCTTGAGTATGGTGCCAATTCGTCTATAGCCAGTGATTTAGGGGCTACAGCTCTTCATCATTCCGCTGGACTAGGAAACATTGAGTTGCTGAGGTACTTAATTTCCAAGTGTGTTGATGTCAATTCACAAAGTGATGCTGGTTCTCCTTTAATTTGGGCTGCTGGTCATGACCAACTAGATGCTGTGAAAGTTCTGTTAGAACACCAGGCTAATCCTAATGTTGAAAGTGATGATGATATTACTCCATTGTTGTCATCTGTGGCAGCTGGTTCACTGGCATGCTTGGAGTTATTGATTCAGGCAGGTGCCAAAGTAAATATGAGTGCTGGTGGAGCGACCCCTTTACACATCGCTGCTGACATTGAGAACTTGGAGATTATAAAATGTTTGTTAAAAGCTGGAGCTGATCCCAGTGTAACTGATGAGGTGAGGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 Ank_5 DUF1685 TPR_17 TPR_8

Protein Analysis

211

Amino Acids

22.42

Weight (kDa)

4.52

Isoelectric Point (pI)

37.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 21 - 71 4.5e-10 Ankyrin repeats (3 copies)
Ank_4 PF13637 22 - 66 5.4e-08 Ankyrin repeats (many copies)
Ank PF00023 45 - 73 7.2e-07 Ankyrin repeat
Ank_4 PF13637 50 - 99 2.7e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 77 - 140 8.3e-12 Ankyrin repeats (3 copies)
Ank_2 PF12796 115 - 206 4.3e-16 Ankyrin repeats (3 copies)
Ank_4 PF13637 115 - 164 1.7e-06 Ankyrin repeats (many copies)
Ank_4 PF13637 154 - 196 1.2e-06 Ankyrin repeats (many copies)
Ank PF00023 176 - 206 1.1e-07 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 575
AarI CACCTGC 1 cut(s) 488
Acc36I ACCTGC 1 cut(s) 488
AccB1I GGYRCC 2 cut(s) 206, 500
AciI CCGC 1 cut(s) 258
AclWI GGATC 2 cut(s) 32, 596
AdeI CACNNNGTG 1 cut(s) 326
AfaI GTAC 2 cut(s) 89, 290
AgsI TTSAA 2 cut(s) 106, 419
AjnI CCWGG 1 cut(s) 399
AluBI AGCT 5 cut(s) 37, 245, 461, 593, 599
AluI AGCT 5 cut(s) 37, 245, 461, 593, 599
AlwI GGATC 2 cut(s) 32, 596
AoxI GGCC 2 cut(s) 67, 169
ApeKI GCWGC 4 cut(s) 158, 353, 458, 548
AspA2I CCTAGG 1 cut(s) 166
AspS9I GGNCC 1 cut(s) 67
AsuHPI GGTGA 2 cut(s) 16, 155
AvrII CCTAGG 1 cut(s) 166
BanI GGYRCC 2 cut(s) 206, 500
BbvCI CCTCAGC 1 cut(s) 284
BbvI GCAGC 4 cut(s) 145, 340, 470, 535
BccI CCATC 1 cut(s) 128
BceAI ACGGC 1 cut(s) 3
BciT130I CCWGG 1 cut(s) 401
BfaI CTAG 3 cut(s) 167, 266, 371
BfmI CTRYAG 2 cut(s) 219, 240
BfuAI ACCTGC 1 cut(s) 488
BisI GCNGC 4 cut(s) 159, 354, 459, 549
BlnI CCTAGG 1 cut(s) 166
BlsI GCNGC 4 cut(s) 160, 355, 460, 550
BmcAI AGTACT 1 cut(s) 89
Bme1390I CCNGG 1 cut(s) 401
BmgT120I GGNCC 1 cut(s) 67
BmiI GGNNCC 2 cut(s) 208, 502
BmrFI CCNGG 1 cut(s) 401
BmrI ACTGGG 1 cut(s) 600
BmsI GCATC 2 cut(s) 319, 364
BmuI ACTGGG 1 cut(s) 600
BpmI CTGGAG 2 cut(s) 116, 615
Bpu10I CCTNAGC 1 cut(s) 284
BpuEI CTTGAG 1 cut(s) 218
BsaJI CCNNGG 1 cut(s) 166
Bse1I ACTGG 3 cut(s) 225, 474, 606
BseBI CCWGG 1 cut(s) 401
BseDI CCNNGG 1 cut(s) 166
BseMII CTCAG 2 cut(s) 66, 275
BseNI ACTGG 3 cut(s) 225, 474, 606
BseXI GCAGC 4 cut(s) 145, 340, 470, 535
BshFI GGCC 2 cut(s) 69, 171
BshNI GGYRCC 2 cut(s) 206, 500
BsnI GGCC 2 cut(s) 69, 171
Bsp143I GATC 4 cut(s) 6, 24, 39, 601
BspACI CCGC 1 cut(s) 258
BspANI GGCC 2 cut(s) 69, 171
BspCNI CTCAG 2 cut(s) 67, 276
BspHI TCATGA 1 cut(s) 361
BspLI GGNNCC 2 cut(s) 208, 502
BspMI ACCTGC 1 cut(s) 488
BspPI GGATC 2 cut(s) 32, 596
BspQI GCTCTTC 2 cut(s) 27, 252
BspT107I GGYRCC 2 cut(s) 206, 500
BsrI ACTGG 3 cut(s) 225, 474, 606
BssECI CCNNGG 1 cut(s) 166
BssMI GATC 4 cut(s) 6, 24, 39, 601
BssT1I CCWWGG 1 cut(s) 166
Bst2UI CCWGG 1 cut(s) 401
Bst4CI ACNGT 1 cut(s) 48
Bst6I CTCTTC 2 cut(s) 27, 252
BstC8I GCNNGC 1 cut(s) 475
BstDEI CTNAG 2 cut(s) 75, 284
BstKTI GATC 4 cut(s) 9, 27, 42, 604
BstMBI GATC 4 cut(s) 6, 24, 39, 601
BstMWI GCNNNNNNNGC 1 cut(s) 527
BstNI CCWGG 1 cut(s) 401
BstNSI RCATGY 1 cut(s) 477
BstSCI CCNGG 1 cut(s) 399
BstSFI CTRYAG 2 cut(s) 219, 240
BstV1I GCAGC 4 cut(s) 145, 340, 470, 535
BsuRI GGCC 2 cut(s) 69, 171
BtgZI GCGATG 1 cut(s) 529
BtsIMutI CAGTG 4 cut(s) 34, 232, 467, 613
BveI ACCTGC 1 cut(s) 488
Cac8I GCNNGC 1 cut(s) 475
CciI TCATGA 1 cut(s) 361
Cfr13I GGNCC 1 cut(s) 67
Csp6I GTAC 2 cut(s) 88, 289
CviAII CATG 3 cut(s) 155, 362, 474
CviQI GTAC 2 cut(s) 88, 289
DdeI CTNAG 2 cut(s) 75, 284
DpnI GATC 4 cut(s) 8, 26, 41, 603
DpnII GATC 4 cut(s) 6, 24, 39, 601
DraIII CACNNNGTG 1 cut(s) 326
Eam1104I CTCTTC 2 cut(s) 27, 252
EarI CTCTTC 2 cut(s) 27, 252
Eco130I CCWWGG 1 cut(s) 166
EcoRII CCWGG 1 cut(s) 399
EcoT14I CCWWGG 1 cut(s) 166
ErhI CCWWGG 1 cut(s) 166
FaeI CATG 3 cut(s) 158, 365, 477
FatI CATG 3 cut(s) 154, 361, 473
Fnu4HI GCNGC 4 cut(s) 159, 354, 459, 549
Fsp4HI GCNGC 4 cut(s) 159, 354, 459, 549
FspBI CTAG 3 cut(s) 167, 266, 371
GluI GCNGC 4 cut(s) 159, 354, 459, 549
GsuI CTGGAG 2 cut(s) 116, 615
HaeIII GGCC 2 cut(s) 69, 171
Hin1II CATG 3 cut(s) 158, 365, 477
HinfI GANTC 1 cut(s) 490
HphI GGTGA 2 cut(s) 16, 155
Hpy166II GTNNAC 1 cut(s) 467
Hpy188I TCNGA 1 cut(s) 24
Hpy188III TCNNGA 2 cut(s) 197, 362
Hpy8I GTNNAC 1 cut(s) 467
Hpy99I CGWCG 1 cut(s) 24
HpyCH4III ACNGT 1 cut(s) 48
HpyCH4V TGCA 1 cut(s) 84
HpyF10VI GCNNNNNNNGC 1 cut(s) 527
HpyF3I CTNAG 2 cut(s) 75, 284
Hsp92II CATG 3 cut(s) 158, 365, 477
Kzo9I GATC 4 cut(s) 6, 24, 39, 601
LguI GCTCTTC 2 cut(s) 27, 252
LmnI GCTCC 2 cut(s) 527, 596
Lsp1109I GCAGC 4 cut(s) 145, 340, 470, 535
LweI GCATC 2 cut(s) 319, 364
MaeI CTAG 3 cut(s) 167, 266, 371
MaeIII GTNAC 1 cut(s) 610
MalI GATC 4 cut(s) 8, 26, 41, 603
MboI GATC 4 cut(s) 6, 24, 39, 601
MboII GAAGA 3 cut(s) 44, 143, 239
MfeI CAATTG 1 cut(s) 51
MluCI AATT 7 cut(s) 51, 101, 108, 211, 294, 316, 345
MnlI CCTC 5 cut(s) 58, 91, 279, 613, 618
MseI TTAA 3 cut(s) 293, 344, 587
MslI CAYNNNNRTG 1 cut(s) 472
MspA1I CMGCKG 2 cut(s) 260, 461
MspR9I CCNGG 1 cut(s) 401
MunI CAATTG 1 cut(s) 51
MvaI CCWGG 1 cut(s) 401
MwoI GCNNNNNNNGC 1 cut(s) 527
NdeII GATC 4 cut(s) 6, 24, 39, 601
NlaIII CATG 3 cut(s) 158, 365, 477
NlaIV GGNNCC 2 cut(s) 208, 502
NspI RCATGY 1 cut(s) 477
PaeI GCATGC 1 cut(s) 477
PagI TCATGA 1 cut(s) 361
PaqCI CACCTGC 1 cut(s) 488
PciSI GCTCTTC 2 cut(s) 27, 252
PfeI GAWTC 1 cut(s) 490
PkrI GCNGC 4 cut(s) 160, 355, 460, 550
PsiI TTATAA 1 cut(s) 575
Psp6I CCWGG 1 cut(s) 399
PspGI CCWGG 1 cut(s) 399
PspN4I GGNNCC 2 cut(s) 208, 502
PspPI GGNCC 1 cut(s) 67
PvuII CAGCTG 1 cut(s) 461
RsaI GTAC 2 cut(s) 89, 290
RsaNI GTAC 2 cut(s) 88, 289
RseI CAYNNNNRTG 1 cut(s) 472
SapI GCTCTTC 2 cut(s) 27, 252
SaqAI TTAA 3 cut(s) 293, 344, 587
SatI GCNGC 4 cut(s) 159, 354, 459, 549
Sau3AI GATC 4 cut(s) 6, 24, 39, 601
Sau96I GGNCC 1 cut(s) 67
ScaI AGTACT 1 cut(s) 89
ScrFI CCNGG 1 cut(s) 401
SetI ASST 9 cut(s) 39, 168, 247, 290, 463, 502, 595, 601, 624
SfaNI GCATC 2 cut(s) 319, 364
SfcI CTRYAG 2 cut(s) 219, 240
SmiMI CAYNNNNRTG 1 cut(s) 472
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
SphI GCATGC 1 cut(s) 477
Sse9I AATT 7 cut(s) 51, 101, 108, 211, 294, 316, 345
SsiI CCGC 1 cut(s) 258
SspMI CTAG 3 cut(s) 167, 266, 371
StyD4I CCNGG 1 cut(s) 399
StyI CCWWGG 1 cut(s) 166
TaaI ACNGT 1 cut(s) 48
TaqI TCGA 1 cut(s) 42
TasI AATT 7 cut(s) 51, 101, 108, 211, 294, 316, 345
TatI WGTACW 1 cut(s) 87
TfiI GAWTC 1 cut(s) 490
Tru1I TTAA 3 cut(s) 293, 344, 587
Tru9I TTAA 3 cut(s) 293, 344, 587
TscAI CASTG 4 cut(s) 34, 232, 474, 613
TseI GCWGC 4 cut(s) 158, 353, 458, 548
TspDTI ATGAA 3 cut(s) 143, 144, 239
TspRI CASTG 4 cut(s) 34, 232, 474, 613
XceI RCATGY 1 cut(s) 477
XmaJI CCTAGG 1 cut(s) 166
XspI CTAG 3 cut(s) 167, 266, 371
ZrmI AGTACT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.