Rh4BG215400

ankyrin repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0002248
Physical Location & Seq
Forward (+)
37471298 .. 37476740
5443 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG215400.1

Sequence Viewer

Length: 687 bp
ATGGCGAAGATGTTCAGCTCAATTTGCTCAATTGAGTTAGCGGAGAAACTTGATGAAGGGAAGGGATTGGCCAAAACTCTGGCTGATATTACGGATGCCAACAAGCGTGGCTCGCTTTATTTTGCTGCAAGAGAGGGCCAGACTGAGGTGTGCAAGTACTTGCTGGAGGAATTGAAAATTGATGTTGATACCAAAGATGAAGATGGCGACACTCCCCTTATTCATGCTGCTCGCCTAGGCCATACTGATATTGCCAAACTGCTTTTGGAGTATGGTGCCAATCCGTCTATAGCCAGTTATTTAGGGGCTACAGCTCTTCATCATTCCGCTGGACTAGGAAACATTGAGTTGCTGAGCGTAAAAATAGTGCCTAATGCTAAAAGTGATGATGATATTACTCCATTGTTGTCATCTGTGGCAATTGTTTCACTGGCGTGCTTGGATTTATTGATTCAGGCAGGTGACAAAGTAAATATGAGTGCTAGTGGAGCGAACCCTTTACACATCGCTGCTGATATTGGGAACTTGGAGATTATAAAATGTTTGTTAAAAGCTGGAGCTGATCCCAGTGTAACTGATGAGGTCTGCTACTGTGCCTATCTATCTCATTCAAAGGGCAAGACACTTCCTTTTATTATTGTTGAAGAAGGGTTGATTTTCAAATTTGAGTTGTCAATGTTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 Ank_5 DUF1685 TPR_17 TPR_8

Protein Analysis

228

Amino Acids

24.32

Weight (kDa)

4.87

Isoelectric Point (pI)

25.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 28 - 96 5.5e-15 Ankyrin repeats (3 copies)
Ank_4 PF13637 39 - 89 3.1e-10 Ankyrin repeats (many copies)
Ank PF00023 68 - 97 2.3e-08 Ankyrin repeat
Ank_4 PF13637 74 - 118 5.9e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 106 - 193 8.3e-09 Ankyrin repeats (3 copies)
Ank PF00023 163 - 193 1.6e-07 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 536
AarI CACCTGC 1 cut(s) 449
Acc36I ACCTGC 1 cut(s) 449
AccB1I GGYRCC 1 cut(s) 275
AciI CCGC 2 cut(s) 41, 327
AclWI GGATC 1 cut(s) 557
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 1 cut(s) 662
AfaI GTAC 1 cut(s) 158
AfiI CCNNNNNNNGG 1 cut(s) 145
AgsI TTSAA 4 cut(s) 175, 612, 644, 661
AleI CACNNNNGTG 1 cut(s) 433
AluBI AGCT 4 cut(s) 18, 314, 554, 560
AluI AGCT 4 cut(s) 18, 314, 554, 560
AlwI GGATC 1 cut(s) 557
AoxI GGCC 3 cut(s) 69, 136, 238
ApeKI GCWGC 3 cut(s) 125, 227, 509
ApoI RAATTY 1 cut(s) 662
Asp700I GAANNNNTTC 1 cut(s) 11
AspA2I CCTAGG 1 cut(s) 235
AspS9I GGNCC 1 cut(s) 136
AsuHPI GGTGA 1 cut(s) 473
AvrII CCTAGG 1 cut(s) 235
BalI TGGCCA 1 cut(s) 71
BanI GGYRCC 1 cut(s) 275
BbvI GCAGC 3 cut(s) 112, 214, 496
BccI CCATC 1 cut(s) 197
BfaI CTAG 3 cut(s) 236, 335, 483
BfmI CTRYAG 2 cut(s) 288, 309
BfuAI ACCTGC 1 cut(s) 449
BisI GCNGC 3 cut(s) 126, 228, 510
BlnI CCTAGG 1 cut(s) 235
BlpI GCTNAGC 1 cut(s) 353
BlsI GCNGC 3 cut(s) 127, 229, 511
BmcAI AGTACT 1 cut(s) 158
BmgT120I GGNCC 1 cut(s) 136
BmiI GGNNCC 1 cut(s) 277
BmrI ACTGGG 1 cut(s) 561
BmsI GCATC 1 cut(s) 85
BmuI ACTGGG 1 cut(s) 561
BpmI CTGGAG 2 cut(s) 185, 576
Bpu1102I GCTNAGC 1 cut(s) 353
BsaJI CCNNGG 1 cut(s) 235
Bsc4I CCNNNNNNNGG 1 cut(s) 145
Bse1I ACTGG 3 cut(s) 294, 435, 567
BseDI CCNNGG 1 cut(s) 235
BseGI GGATG 1 cut(s) 100
BseLI CCNNNNNNNGG 1 cut(s) 145
BseMII CTCAG 2 cut(s) 135, 344
BseNI ACTGG 3 cut(s) 294, 435, 567
BseXI GCAGC 3 cut(s) 112, 214, 496
BshFI GGCC 3 cut(s) 71, 138, 240
BshNI GGYRCC 1 cut(s) 275
BslI CCNNNNNNNGG 1 cut(s) 145
BsnI GGCC 3 cut(s) 71, 138, 240
Bsp143I GATC 1 cut(s) 562
Bsp1720I GCTNAGC 1 cut(s) 353
BspACI CCGC 2 cut(s) 41, 327
BspANI GGCC 3 cut(s) 71, 138, 240
BspCNI CTCAG 2 cut(s) 136, 345
BspLI GGNNCC 1 cut(s) 277
BspMI ACCTGC 1 cut(s) 449
BspPI GGATC 1 cut(s) 557
BspQI GCTCTTC 1 cut(s) 321
BspT107I GGYRCC 1 cut(s) 275
BsrI ACTGG 3 cut(s) 294, 435, 567
BssECI CCNNGG 1 cut(s) 235
BssMI GATC 1 cut(s) 562
BssT1I CCWWGG 1 cut(s) 235
Bst4CI ACNGT 1 cut(s) 593
Bst6I CTCTTC 1 cut(s) 321
BstC8I GCNNGC 3 cut(s) 113, 232, 436
BstDEI CTNAG 2 cut(s) 144, 353
BstF5I GGATG 1 cut(s) 100
BstKTI GATC 1 cut(s) 565
BstMBI GATC 1 cut(s) 562
BstMWI GCNNNNNNNGC 3 cut(s) 24, 112, 488
BstSFI CTRYAG 2 cut(s) 288, 309
BstV1I GCAGC 3 cut(s) 112, 214, 496
BstXI CCANNNNNNTGG 1 cut(s) 79
BsuRI GGCC 3 cut(s) 71, 138, 240
BtgZI GCGATG 1 cut(s) 490
BtsCI GGATG 1 cut(s) 100
BtsIMutI CAGTG 2 cut(s) 428, 574
BveI ACCTGC 1 cut(s) 449
Cac8I GCNNGC 3 cut(s) 113, 232, 436
Cfr13I GGNCC 1 cut(s) 136
Csp6I GTAC 1 cut(s) 157
CspCI CAANNNNNGTGG 2 cut(s) 88, 123
CviAII CATG 1 cut(s) 224
CviQI GTAC 1 cut(s) 157
DdeI CTNAG 2 cut(s) 144, 353
DpnI GATC 1 cut(s) 564
DpnII GATC 1 cut(s) 562
EaeI YGGCCR 1 cut(s) 69
Eam1104I CTCTTC 1 cut(s) 321
EarI CTCTTC 1 cut(s) 321
Eco130I CCWWGG 1 cut(s) 235
EcoT14I CCWWGG 1 cut(s) 235
ErhI CCWWGG 1 cut(s) 235
FaeI CATG 1 cut(s) 227
FaiI YATR 6 cut(s) 225, 243, 273, 290, 476, 536
FatI CATG 1 cut(s) 223
Fnu4HI GCNGC 3 cut(s) 126, 228, 510
FokI GGATG 1 cut(s) 107
Fsp4HI GCNGC 3 cut(s) 126, 228, 510
FspBI CTAG 3 cut(s) 236, 335, 483
GluI GCNGC 3 cut(s) 126, 228, 510
GsuI CTGGAG 2 cut(s) 185, 576
HaeIII GGCC 3 cut(s) 71, 138, 240
Hin1II CATG 1 cut(s) 227
HinfI GANTC 1 cut(s) 451
HphI GGTGA 1 cut(s) 473
HpyAV CCTTC 3 cut(s) 50, 55, 641
HpyCH4III ACNGT 1 cut(s) 593
HpyCH4V TGCA 2 cut(s) 128, 153
HpyF10VI GCNNNNNNNGC 3 cut(s) 24, 112, 488
HpyF3I CTNAG 2 cut(s) 144, 353
Hsp92II CATG 1 cut(s) 227
Kzo9I GATC 1 cut(s) 562
LguI GCTCTTC 1 cut(s) 321
LmnI GCTCC 2 cut(s) 488, 557
Lsp1109I GCAGC 3 cut(s) 112, 214, 496
LweI GCATC 1 cut(s) 85
MaeI CTAG 3 cut(s) 236, 335, 483
MaeIII GTNAC 2 cut(s) 461, 571
MalI GATC 1 cut(s) 564
MboI GATC 1 cut(s) 562
MboII GAAGA 4 cut(s) 19, 212, 308, 656
MfeI CAATTG 2 cut(s) 30, 420
MlsI TGGCCA 1 cut(s) 71
MluCI AATT 6 cut(s) 21, 30, 170, 177, 420, 662
MluNI TGGCCA 1 cut(s) 71
MnlI CCTC 4 cut(s) 127, 139, 160, 574
Mox20I TGGCCA 1 cut(s) 71
MroXI GAANNNNTTC 1 cut(s) 11
MscI TGGCCA 1 cut(s) 71
MseI TTAA 1 cut(s) 548
MslI CAYNNNNRTG 1 cut(s) 433
Msp20I TGGCCA 1 cut(s) 71
MspA1I CMGCKG 1 cut(s) 329
MunI CAATTG 2 cut(s) 30, 420
MwoI GCNNNNNNNGC 3 cut(s) 24, 112, 488
NdeII GATC 1 cut(s) 562
NlaIII CATG 1 cut(s) 227
NlaIV GGNNCC 1 cut(s) 277
NmuCI GTSAC 1 cut(s) 461
OliI CACNNNNGTG 1 cut(s) 433
PaqCI CACCTGC 1 cut(s) 449
PciSI GCTCTTC 1 cut(s) 321
PdmI GAANNNNTTC 1 cut(s) 11
PfeI GAWTC 1 cut(s) 451
PkrI GCNGC 3 cut(s) 127, 229, 511
PsiI TTATAA 1 cut(s) 536
PspN4I GGNNCC 1 cut(s) 277
PspPI GGNCC 1 cut(s) 136
RsaI GTAC 1 cut(s) 158
RsaNI GTAC 1 cut(s) 157
RseI CAYNNNNRTG 1 cut(s) 433
SapI GCTCTTC 1 cut(s) 321
SaqAI TTAA 1 cut(s) 548
SatI GCNGC 3 cut(s) 126, 228, 510
Sau3AI GATC 1 cut(s) 562
Sau96I GGNCC 1 cut(s) 136
ScaI AGTACT 1 cut(s) 158
SetI ASST 7 cut(s) 20, 150, 316, 463, 556, 562, 585
SfaNI GCATC 1 cut(s) 85
SfcI CTRYAG 2 cut(s) 288, 309
SmiMI CAYNNNNRTG 1 cut(s) 433
Sse9I AATT 6 cut(s) 21, 30, 170, 177, 420, 662
SsiI CCGC 2 cut(s) 41, 327
SspMI CTAG 3 cut(s) 236, 335, 483
StyI CCWWGG 1 cut(s) 235
TaaI ACNGT 1 cut(s) 593
TasI AATT 6 cut(s) 21, 30, 170, 177, 420, 662
TatI WGTACW 1 cut(s) 156
TfiI GAWTC 1 cut(s) 451
Tru1I TTAA 1 cut(s) 548
Tru9I TTAA 1 cut(s) 548
TscAI CASTG 2 cut(s) 435, 574
TseFI GTSAC 1 cut(s) 461
TseI GCWGC 3 cut(s) 125, 227, 509
Tsp45I GTSAC 1 cut(s) 461
TspDTI ATGAA 4 cut(s) 69, 212, 213, 308
TspGWI ACGGA 2 cut(s) 107, 273
TspRI CASTG 2 cut(s) 435, 574
XapI RAATTY 1 cut(s) 662
XcmI CCANNNNNNNNNTGG 1 cut(s) 262
XmaJI CCTAGG 1 cut(s) 235
XmnI GAANNNNTTC 1 cut(s) 11
XspI CTAG 3 cut(s) 236, 335, 483
ZrmI AGTACT 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.