Rh3AG030000

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0002248
Physical Location & Seq
Forward (+)
2009260 .. 2014268
5009 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG030000.1

Sequence Viewer

Length: 1113 bp
ATGGCCCCTGACGCTTCTGATGCACTTGCAGCTAGGGAAATAGTGCAGCAGTTTCTGAATGCTGCATGTACTGGGGATGTTGATCTCTTAAAGAAGTTGGCGGGGAAACTTGATGAAGGGAAGGGATTGGCCAAAACTGTGGCTGATATTAAGGATGCCAACAAGCGCGGCGCGCTTCATTTTGCTGCAAGAGAGGGCCAGTCTGAGATGTGCAAGTACTTGCTGGAGGAATTGAAACTTGATGTTGATACCAAAGACGAAGATGGCGACACTCCCCTTATTCATGCTGCTCGCCTAGGCCATACTGATACTGCAAAACTGCTTCTTGAGTATGGTGCCAATCCGTCTATAGCCAGTGATTTAGGGGCTACAGCTCTTCATCATTCCGCTGGACTAGGAAACATTGAGTTGCTGAGGTACTTAATTTCCAAGGGTGTCGATGTCAATTCACAAAGTGATGCTGGTTCTCCTTTAATTTGGGCTGCTGGTCATGACCAACCAGATGCTGTGAAAGTTCTGTTAGAACACCACGCTAATCCTAATGCTGAAAGTGATGATGATATTACTCCATTGTTGTCATCTGTGGCAGCTGGTTCACTGGCATGCTTGGAGTTATTGATTCAGGCAGGTGCCAAAGTAAATATGAGTGCTGGTGGAGCGACTCCTTTACACATTGCTGCTGATATTGGGAACTTGGAGATTATAAAATGTTTGTTAAAAGCTGGAGCTGATCCCAATGTAACTGATGAGGAGGGTTTGAAGCCAATACAGGTTGCAGCTGCAAGTGGGAACCGAAGCGCTGTGGAGATCCTCTTTCCCTTGACATCACAAGTTGAAACCATTTCAAAATGGACAGTTGACGGCATACTTGAGTATATGCAGTCTGAAACAAGCAGACAACAGGAACAACCGCGAAATACTAAGGAACTTAATGTCCCAAAAGACTCTACTTCACTGAAGAAAGAGCTCCCTGAGGTGACACCTGAGGCAAAGAAAAGAGCCGCAGAAGCAAAATTGAGAGGAGGCGATGCTTTCAGTAGAAAGGATTATCAAGCGGCTGTTGATGCATACACGCAGGTGCATTATATTTCTTTCTCAGTTTTTAGTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 Ank_5 DUF1685 TPR_17 TPR_8

Protein Analysis

370

Amino Acids

39.36

Weight (kDa)

5.03

Isoelectric Point (pI)

32.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 20 - 116 5.4e-17 Ankyrin repeats (3 copies)
Ank_5 PF13857 58 - 96 9.6e-06 Ankyrin repeats (many copies)
Ank_4 PF13637 59 - 101 2.3e-06 Ankyrin repeats (many copies)
Ank PF00023 88 - 118 1.7e-07 Ankyrin repeat
Ank_4 PF13637 94 - 142 3.8e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 120 - 183 8.7e-12 Ankyrin repeats (3 copies)
Ank_4 PF13637 158 - 207 8.8e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 195 - 273 8.6e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 197 - 239 5.1e-07 Ankyrin repeats (many copies)
Ank_5 PF13857 206 - 259 1e-08 Ankyrin repeats (many copies)
Ank PF00023 219 - 249 2.8e-09 Ankyrin repeat
Ank_3 PF13606 219 - 247 2.8e-06 Ankyrin repeat
Ank_4 PF13637 230 - 271 4.3e-06 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 704
AarI CACCTGC 2 cut(s) 617, 1066
Acc36I ACCTGC 2 cut(s) 617, 1066
AccB1I GGYRCC 2 cut(s) 335, 629
AccII CGCG 3 cut(s) 168, 173, 913
AciI CCGC 6 cut(s) 101, 168, 387, 911, 1002, 1055
AclWI GGATC 2 cut(s) 725, 802
AcoI YGGCCR 1 cut(s) 129
AcuI CTGAAG 1 cut(s) 977
AdeI CACNNNGTG 1 cut(s) 455
AfaI GTAC 3 cut(s) 70, 218, 419
AfeI AGCGCT 1 cut(s) 799
AgsI TTSAA 4 cut(s) 235, 760, 836, 846
AleI CACNNNNGTG 1 cut(s) 1076
AloI GAACNNNNNNTCC 2 cut(s) 918, 950
AluBI AGCT 7 cut(s) 32, 374, 590, 722, 728, 779, 967
AluI AGCT 7 cut(s) 32, 374, 590, 722, 728, 779, 967
Alw21I GWGCWC 1 cut(s) 969
AlwI GGATC 2 cut(s) 725, 802
AlwNI CAGNNNCTG 2 cut(s) 55, 506
Aor51HI AGCGCT 1 cut(s) 799
AoxI GGCC 4 cut(s) 3, 129, 196, 298
AspA2I CCTAGG 1 cut(s) 295
AspLEI GCGC 4 cut(s) 168, 173, 175, 800
AspS9I GGNCC 2 cut(s) 4, 196
AsuHPI GGTGA 1 cut(s) 988
AvrII CCTAGG 1 cut(s) 295
AxyI CCTNAGG 2 cut(s) 972, 984
BalI TGGCCA 1 cut(s) 131
BanI GGYRCC 2 cut(s) 335, 629
BanII GRGCYC 1 cut(s) 969
Bbv12I GWGCWC 1 cut(s) 969
BbvCI CCTCAGC 1 cut(s) 413
BccI CCATC 1 cut(s) 257
BceAI ACGGC 1 cut(s) 877
BfaI CTAG 3 cut(s) 33, 296, 395
BfmI CTRYAG 2 cut(s) 348, 369
BfoI RGCGCY 1 cut(s) 801
BfuAI ACCTGC 2 cut(s) 617, 1066
BlnI CCTAGG 1 cut(s) 295
BmcAI AGTACT 1 cut(s) 218
BmgT120I GGNCC 2 cut(s) 4, 196
BmiI GGNNCC 4 cut(s) 6, 337, 631, 791
BmrI ACTGGG 1 cut(s) 81
BmsI GCATC 6 cut(s) 10, 145, 448, 493, 1018, 1054
BmuI ACTGGG 1 cut(s) 81
BpmI CTGGAG 2 cut(s) 245, 744
Bpu10I CCTNAGC 1 cut(s) 413
BpuEI CTTGAG 2 cut(s) 347, 890
BsaBI GATNNNNATC 1 cut(s) 81
BsaJI CCNNGG 2 cut(s) 295, 429
Bse1I ACTGG 4 cut(s) 76, 199, 354, 603
Bse21I CCTNAGG 2 cut(s) 972, 984
Bse3DI GCAATG 1 cut(s) 672
Bse8I GATNNNNATC 1 cut(s) 81
BseDI CCNNGG 2 cut(s) 295, 429
BseGI GGATG 2 cut(s) 82, 160
BseJI GATNNNNATC 1 cut(s) 81
BseMI GCAATG 1 cut(s) 672
BseMII CTCAG 5 cut(s) 195, 404, 963, 975, 1110
BseNI ACTGG 4 cut(s) 76, 199, 354, 603
BsePI GCGCGC 1 cut(s) 171
BseRI GAGGAG 2 cut(s) 764, 1035
BsgI GTGCAG 1 cut(s) 65
Bsh1236I CGCG 3 cut(s) 168, 173, 913
BshFI GGCC 4 cut(s) 5, 131, 198, 300
BshNI GGYRCC 2 cut(s) 335, 629
BsiHKAI GWGCWC 1 cut(s) 969
BslFI GGGAC 1 cut(s) 920
BsmFI GGGAC 1 cut(s) 920
BsmI GAATGC 1 cut(s) 64
BsnI GGCC 4 cut(s) 5, 131, 198, 300
Bsp1286I GDGCHC 1 cut(s) 969
Bsp143I GATC 3 cut(s) 82, 730, 807
BspACI CCGC 6 cut(s) 101, 168, 387, 911, 1002, 1055
BspANI GGCC 4 cut(s) 5, 131, 198, 300
BspCNI CTCAG 5 cut(s) 196, 405, 964, 976, 1109
BspFNI CGCG 3 cut(s) 168, 173, 913
BspHI TCATGA 1 cut(s) 490
BspLI GGNNCC 4 cut(s) 6, 337, 631, 791
BspMI ACCTGC 2 cut(s) 617, 1066
BspPI GGATC 2 cut(s) 725, 802
BspQI GCTCTTC 1 cut(s) 381
BspT107I GGYRCC 2 cut(s) 335, 629
BsrDI GCAATG 1 cut(s) 672
BsrI ACTGG 4 cut(s) 76, 199, 354, 603
BssECI CCNNGG 2 cut(s) 295, 429
BssHII GCGCGC 1 cut(s) 171
BssMI GATC 3 cut(s) 82, 730, 807
BssT1I CCWWGG 2 cut(s) 295, 429
Bst4CI ACNGT 2 cut(s) 139, 856
Bst6I CTCTTC 1 cut(s) 381
BstC8I GCNNGC 3 cut(s) 173, 292, 604
BstDEI CTNAG 6 cut(s) 204, 413, 921, 972, 984, 1096
BstF5I GGATG 2 cut(s) 82, 160
BstFNI CGCG 3 cut(s) 168, 173, 913
BstH2I RGCGCY 1 cut(s) 801
BstHHI GCGC 4 cut(s) 168, 173, 175, 800
BstKTI GATC 3 cut(s) 85, 733, 810
BstMBI GATC 3 cut(s) 82, 730, 807
BstMWI GCNNNNNNNGC 7 cut(s) 11, 20, 29, 172, 656, 1007, 1064
BstNSI RCATGY 2 cut(s) 69, 606
BstSFI CTRYAG 2 cut(s) 348, 369
BstUI CGCG 3 cut(s) 168, 173, 913
BstX2I RGATCY 1 cut(s) 807
BstXI CCANNNNNNTGG 1 cut(s) 139
BstYI RGATCY 1 cut(s) 807
Bsu36I CCTNAGG 2 cut(s) 972, 984
BsuRI GGCC 4 cut(s) 5, 131, 198, 300
BtgZI GCGATG 1 cut(s) 1041
BtsCI GGATG 2 cut(s) 82, 160
BtsIMutI CAGTG 3 cut(s) 361, 596, 953
BveI ACCTGC 2 cut(s) 617, 1066
Cac8I GCNNGC 3 cut(s) 173, 292, 604
CaiI CAGNNNCTG 2 cut(s) 55, 506
CciI TCATGA 1 cut(s) 490
CfoI GCGC 4 cut(s) 168, 173, 175, 800
Cfr13I GGNCC 2 cut(s) 4, 196
CseI GACGC 1 cut(s) 20
Csp6I GTAC 3 cut(s) 69, 217, 418
CviAII CATG 4 cut(s) 66, 284, 491, 603
CviQI GTAC 3 cut(s) 69, 217, 418
DdeI CTNAG 6 cut(s) 204, 413, 921, 972, 984, 1096
DpnI GATC 3 cut(s) 84, 732, 809
DpnII GATC 3 cut(s) 82, 730, 807
DraIII CACNNNGTG 1 cut(s) 455
EaeI YGGCCR 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 381
EarI CTCTTC 1 cut(s) 381
Ecl136II GAGCTC 1 cut(s) 967
Eco130I CCWWGG 2 cut(s) 295, 429
Eco24I GRGCYC 1 cut(s) 969
Eco47III AGCGCT 1 cut(s) 799
Eco53kI GAGCTC 1 cut(s) 967
Eco57I CTGAAG 1 cut(s) 977
Eco81I CCTNAGG 2 cut(s) 972, 984
EcoICRI GAGCTC 1 cut(s) 967
EcoT14I CCWWGG 2 cut(s) 295, 429
EcoT22I ATGCAT 1 cut(s) 1069
EcoT38I GRGCYC 1 cut(s) 969
ErhI CCWWGG 2 cut(s) 295, 429
FaeI CATG 4 cut(s) 69, 287, 494, 606
FaqI GGGAC 1 cut(s) 920
FatI CATG 4 cut(s) 65, 283, 490, 602
FauI CCCGC 1 cut(s) 94
FokI GGATG 2 cut(s) 89, 167
FriOI GRGCYC 1 cut(s) 969
FspBI CTAG 3 cut(s) 33, 296, 395
GlaI GCGC 4 cut(s) 167, 172, 174, 799
GsuI CTGGAG 2 cut(s) 245, 744
HaeII RGCGCY 1 cut(s) 801
HaeIII GGCC 4 cut(s) 5, 131, 198, 300
HgaI GACGC 1 cut(s) 20
HhaI GCGC 4 cut(s) 168, 173, 175, 800
Hin1II CATG 4 cut(s) 69, 287, 494, 606
Hin6I GCGC 4 cut(s) 166, 171, 173, 798
HinP1I GCGC 4 cut(s) 166, 171, 173, 798
HincII GTYRAC 1 cut(s) 859
HindII GTYRAC 1 cut(s) 859
HinfI GANTC 3 cut(s) 619, 661, 944
HphI GGTGA 1 cut(s) 988
Hpy166II GTNNAC 2 cut(s) 596, 859
Hpy188I TCNGA 4 cut(s) 19, 57, 205, 886
Hpy188III TCNNGA 2 cut(s) 326, 491
Hpy8I GTNNAC 2 cut(s) 596, 859
HpyAV CCTTC 2 cut(s) 110, 115
HpyCH4III ACNGT 2 cut(s) 139, 856
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 20, 29, 172, 656, 1007, 1064
HpyF3I CTNAG 6 cut(s) 204, 413, 921, 972, 984, 1096
Hsp92II CATG 4 cut(s) 69, 287, 494, 606
HspAI GCGC 4 cut(s) 166, 171, 173, 798
Kzo9I GATC 3 cut(s) 82, 730, 807
LguI GCTCTTC 1 cut(s) 381
LmnI GCTCC 3 cut(s) 656, 725, 972
LweI GCATC 6 cut(s) 10, 145, 448, 493, 1018, 1054
MaeI CTAG 3 cut(s) 33, 296, 395
MaeIII GTNAC 2 cut(s) 739, 976
MalI GATC 3 cut(s) 84, 732, 809
MboI GATC 3 cut(s) 82, 730, 807
MboII GAAGA 3 cut(s) 272, 368, 970
MflI RGATCY 1 cut(s) 807
MhlI GDGCHC 1 cut(s) 969
MlsI TGGCCA 1 cut(s) 131
MluCI AATT 5 cut(s) 230, 423, 445, 474, 1013
MluNI TGGCCA 1 cut(s) 131
MlyI GAGTC 2 cut(s) 655, 938
Mox20I TGGCCA 1 cut(s) 131
Mph1103I ATGCAT 1 cut(s) 1069
MscI TGGCCA 1 cut(s) 131
MseI TTAA 7 cut(s) 89, 150, 422, 473, 716, 930, 1111
MslI CAYNNNNRTG 2 cut(s) 601, 1076
Msp20I TGGCCA 1 cut(s) 131
MspA1I CMGCKG 3 cut(s) 389, 590, 779
MteI GCGCNGCGC 1 cut(s) 169
Mva1269I GAATGC 1 cut(s) 64
MvnI CGCG 3 cut(s) 168, 173, 913
MwoI GCNNNNNNNGC 7 cut(s) 11, 20, 29, 172, 656, 1007, 1064
NdeII GATC 3 cut(s) 82, 730, 807
NlaIII CATG 4 cut(s) 69, 287, 494, 606
NlaIV GGNNCC 4 cut(s) 6, 337, 631, 791
NmuCI GTSAC 1 cut(s) 976
NsiI ATGCAT 1 cut(s) 1069
NspI RCATGY 2 cut(s) 69, 606
OliI CACNNNNGTG 1 cut(s) 1076
PaeI GCATGC 1 cut(s) 606
PagI TCATGA 1 cut(s) 490
PaqCI CACCTGC 2 cut(s) 617, 1066
PauI GCGCGC 1 cut(s) 171
PciSI GCTCTTC 1 cut(s) 381
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PctI GAATGC 1 cut(s) 64
PfeI GAWTC 1 cut(s) 619
PleI GAGTC 2 cut(s) 655, 938
PpsI GAGTC 2 cut(s) 655, 938
PsiI TTATAA 1 cut(s) 704
Psp124BI GAGCTC 1 cut(s) 969
PspN4I GGNNCC 4 cut(s) 6, 337, 631, 791
PspPI GGNCC 2 cut(s) 4, 196
PstNI CAGNNNCTG 2 cut(s) 55, 506
PsuI RGATCY 1 cut(s) 807
PteI GCGCGC 1 cut(s) 171
PvuII CAGCTG 2 cut(s) 590, 779
RsaI GTAC 3 cut(s) 70, 218, 419
RsaNI GTAC 3 cut(s) 69, 217, 418
RseI CAYNNNNRTG 2 cut(s) 601, 1076
SacI GAGCTC 1 cut(s) 969
SapI GCTCTTC 1 cut(s) 381
SaqAI TTAA 7 cut(s) 89, 150, 422, 473, 716, 930, 1111
Sau3AI GATC 3 cut(s) 82, 730, 807
Sau96I GGNCC 2 cut(s) 4, 196
ScaI AGTACT 1 cut(s) 218
SchI GAGTC 2 cut(s) 655, 938
SduI GDGCHC 1 cut(s) 969
SfaNI GCATC 6 cut(s) 10, 145, 448, 493, 1018, 1054
SfcI CTRYAG 2 cut(s) 348, 369
SmiMI CAYNNNNRTG 2 cut(s) 601, 1076
SmlI CTYRAG 2 cut(s) 326, 869
SmoI CTYRAG 2 cut(s) 326, 869
SphI GCATGC 1 cut(s) 606
Sse9I AATT 5 cut(s) 230, 423, 445, 474, 1013
SsiI CCGC 6 cut(s) 101, 168, 387, 911, 1002, 1055
SspMI CTAG 3 cut(s) 33, 296, 395
SstI GAGCTC 1 cut(s) 969
StyI CCWWGG 2 cut(s) 295, 429
TaaI ACNGT 2 cut(s) 139, 856
TaqI TCGA 1 cut(s) 438
TasI AATT 5 cut(s) 230, 423, 445, 474, 1013
TatI WGTACW 2 cut(s) 68, 216
TauI GCSGC 3 cut(s) 171, 1004, 1058
TfiI GAWTC 1 cut(s) 619
Tru1I TTAA 7 cut(s) 89, 150, 422, 473, 716, 930, 1111
Tru9I TTAA 7 cut(s) 89, 150, 422, 473, 716, 930, 1111
TscAI CASTG 3 cut(s) 361, 603, 960
TseFI GTSAC 1 cut(s) 976
Tsp45I GTSAC 1 cut(s) 976
TspDTI ATGAA 4 cut(s) 129, 167, 272, 368
TspGWI ACGGA 1 cut(s) 333
TspRI CASTG 3 cut(s) 361, 603, 960
XceI RCATGY 2 cut(s) 69, 606
XmaJI CCTAGG 1 cut(s) 295
XspI CTAG 3 cut(s) 33, 296, 395
ZrmI AGTACT 1 cut(s) 218
Zsp2I ATGCAT 1 cut(s) 1069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.