AT4G34215

Carbohydrate esterase, sialic acid-specific acetylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0003321
Physical Location & Seq
Reverse (-)
16379603 .. 16381449
1847 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G34215.1

Sequence Viewer

Length: 783 bp
ATGGAAGGAGGATCTATCACTCCCGGTGAAGATAAACCGGAGATCCAATCTCCAATTCCACCAAATCAGATCTTCATACTCTCTGGACAAAGCAATATGGCAGGACGCGGCGGCGTCTTCAAAGACCACCACAACAATCGCTGGGTGTGGGATAAAATCCTTCCACCGGAATGCGCACCAAACTCATCGATCCTCCGCCTGTCCGCAGATCTCCGGTGGGAAGAAGCACACGAGCCACTACACGTTGACATTGACACAGGTAAAGTGTGTGGAGTAGGTCCAGGAATGGCGTTCGCTAACGCGGTGAAGAATCGCCTGGAAACAGATTCGGCTGTGATCGGGTTGGTGCCGTGCGCTTCCGGTGGAACGGCGATAAAAGAGTGGGAGCGTGGAAGCCACTTGTACGAGAGGATGGTCAAGAGAACGGAGGAGAGTAGGAAATGCGGCGGAGAGATCAAGGCGGTGTTGTGGTATCAAGGAGAGAGTGACGTGTTGGACATCCATGACGCCGAGAGCTACGGGAACAATATGGATCGTTTGATTAAGAACCTCCGTCATGATCTCAACCTTCCTTCTCTTCCCATTATTCAGGTGGCAATAGCATCGGGAGGAGGATACATAGATAAGGTGAGAGAAGCACAGTTGGGACTGAAACTGTCGAATGTGGTCTGTGTAGATGCTAAGGGATTGCCGCTAAAGTCCGACAATCTTCACTTAACCACCGAGGCTCAAGTCCAGCTTGGTCTCTCCTTAGCTCAAGCTTACCTTTCCAACTTCTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

260

Amino Acids

28.36

Weight (kDa)

5.63

Isoelectric Point (pI)

37.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 22 - 256 6.4e-93 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 175
AccB1I GGYRCC 1 cut(s) 346
AccII CGCG 2 cut(s) 108, 302
AciI CCGC 9 cut(s) 108, 111, 196, 204, 302, 444, 447, 461, 692
AclWI GGATC 4 cut(s) 19, 37, 184, 540
AcyI GRCGYC 2 cut(s) 114, 507
AfaI GTAC 1 cut(s) 404
AfiI CCNNNNNNNGG 1 cut(s) 166
AflIII ACRYGT 2 cut(s) 241, 489
AgsI TTSAA 1 cut(s) 121
AjiI CACGTC 1 cut(s) 490
AjnI CCWGG 2 cut(s) 280, 315
AjuI GAANNNNNNNTTGG 2 cut(s) 39, 71
AluBI AGCT 4 cut(s) 516, 739, 755, 761
AluI AGCT 4 cut(s) 516, 739, 755, 761
Alw26I GTCTC 1 cut(s) 749
AlwI GGATC 4 cut(s) 19, 37, 184, 540
AspLEI GCGC 2 cut(s) 176, 356
AspS9I GGNCC 1 cut(s) 278
AsuC2I CCSGG 1 cut(s) 24
AsuHPI GGTGA 3 cut(s) 38, 316, 640
AvaII GGWCC 1 cut(s) 278
BanI GGYRCC 1 cut(s) 346
BauI CACGAG 1 cut(s) 230
BbsI GAAGAC 1 cut(s) 109
BccI CCATC 1 cut(s) 406
BceAI ACGGC 2 cut(s) 334, 384
BcgI CGANNNNNNTGC 2 cut(s) 585, 619
BciT130I CCWGG 2 cut(s) 282, 317
BciVI GTATCC 1 cut(s) 608
BcnI CCSGG 1 cut(s) 24
BcoDI GTCTC 1 cut(s) 749
BfaI CTAG 1 cut(s) 781
BfuI GTATCC 1 cut(s) 608
BglII AGATCT 2 cut(s) 69, 208
BisI GCNGC 4 cut(s) 109, 112, 445, 692
BlsI GCNGC 4 cut(s) 110, 113, 446, 693
Bme1390I CCNGG 3 cut(s) 24, 282, 317
Bme18I GGWCC 1 cut(s) 278
BmgBI CACGTC 1 cut(s) 490
BmgT120I GGNCC 1 cut(s) 278
BmiI GGNNCC 1 cut(s) 348
BmrFI CCNGG 3 cut(s) 24, 282, 317
BmsI GCATC 2 cut(s) 611, 667
BpiI GAAGAC 1 cut(s) 109
Bpu10I CCTNAGC 2 cut(s) 681, 751
BpuEI CTTGAG 2 cut(s) 714, 741
BpuMI CCSGG 1 cut(s) 24
Bsa29I ATCGAT 1 cut(s) 188
BsaHI GRCGYC 2 cut(s) 114, 507
BsaI GGTCTC 1 cut(s) 749
BsaJI CCNNGG 1 cut(s) 723
BsaWI WCCGGW 4 cut(s) 37, 166, 213, 359
Bsc4I CCNNNNNNNGG 1 cut(s) 166
BseBI CCWGG 2 cut(s) 282, 317
BseCI ATCGAT 1 cut(s) 188
BseDI CCNNGG 1 cut(s) 723
BseGI GGATG 2 cut(s) 417, 498
BseLI CCNNNNNNNGG 1 cut(s) 166
BseRI GAGGAG 2 cut(s) 443, 624
BseYI CCCAGC 1 cut(s) 141
Bsh1236I CGCG 2 cut(s) 108, 302
BshNI GGYRCC 1 cut(s) 346
BshVI ATCGAT 1 cut(s) 188
BsiSI CCGG 5 cut(s) 24, 38, 167, 214, 360
BslFI GGGAC 1 cut(s) 660
BslI CCNNNNNNNGG 1 cut(s) 166
BsmAI GTCTC 1 cut(s) 749
BsmFI GGGAC 1 cut(s) 660
BsmI GAATGC 1 cut(s) 176
Bso31I GGTCTC 1 cut(s) 749
Bsp143I GATC 9 cut(s) 11, 42, 69, 189, 208, 336, 453, 532, 559
BspACI CCGC 9 cut(s) 108, 111, 196, 204, 302, 444, 447, 461, 692
BspDI ATCGAT 1 cut(s) 188
BspFNI CGCG 2 cut(s) 108, 302
BspHI TCATGA 1 cut(s) 556
BspLI GGNNCC 1 cut(s) 348
BspPI GGATC 4 cut(s) 19, 37, 184, 540
BspT107I GGYRCC 1 cut(s) 346
BspTNI GGTCTC 1 cut(s) 749
BssECI CCNNGG 1 cut(s) 723
BssMI GATC 9 cut(s) 11, 42, 69, 189, 208, 336, 453, 532, 559
BssNI GRCGYC 2 cut(s) 114, 507
BssSI CACGAG 1 cut(s) 230
Bst2BI CACGAG 1 cut(s) 230
Bst2UI CCWGG 2 cut(s) 282, 317
Bst4CI ACNGT 2 cut(s) 642, 657
Bst6I CTCTTC 1 cut(s) 582
BstACI GRCGYC 2 cut(s) 114, 507
BstDEI CTNAG 2 cut(s) 681, 751
BstF5I GGATG 2 cut(s) 417, 498
BstFNI CGCG 2 cut(s) 108, 302
BstHHI GCGC 2 cut(s) 176, 356
BstKTI GATC 9 cut(s) 14, 45, 72, 192, 211, 339, 456, 535, 562
BstMAI GTCTC 1 cut(s) 749
BstMBI GATC 9 cut(s) 11, 42, 69, 189, 208, 336, 453, 532, 559
BstNI CCWGG 2 cut(s) 282, 317
BstSCI CCNGG 3 cut(s) 22, 280, 315
BstUI CGCG 2 cut(s) 108, 302
BstV2I GAAGAC 1 cut(s) 109
BstX2I RGATCY 4 cut(s) 11, 42, 69, 208
BstYI RGATCY 4 cut(s) 11, 42, 69, 208
Bsu15I ATCGAT 1 cut(s) 188
BsuI GTATCC 1 cut(s) 608
BsuTUI ATCGAT 1 cut(s) 188
BtrI CACGTC 1 cut(s) 490
BtsCI GGATG 2 cut(s) 417, 498
CciI TCATGA 1 cut(s) 556
CfoI GCGC 2 cut(s) 176, 356
Cfr13I GGNCC 1 cut(s) 278
ClaI ATCGAT 1 cut(s) 188
CseI GACGC 3 cut(s) 103, 114, 515
Csp6I GTAC 1 cut(s) 403
CviAII CATG 2 cut(s) 503, 557
CviJI RGCY 8 cut(s) 235, 332, 396, 516, 728, 739, 755, 761
CviKI_1 RGCY 8 cut(s) 235, 332, 396, 516, 728, 739, 755, 761
CviQI GTAC 1 cut(s) 403
DdeI CTNAG 2 cut(s) 681, 751
DpnI GATC 9 cut(s) 13, 44, 71, 191, 210, 338, 455, 534, 561
DpnII GATC 9 cut(s) 11, 42, 69, 189, 208, 336, 453, 532, 559
Eam1104I CTCTTC 1 cut(s) 582
EarI CTCTTC 1 cut(s) 582
EciI GGCGGA 2 cut(s) 185, 462
Eco31I GGTCTC 1 cut(s) 749
Eco47I GGWCC 1 cut(s) 278
EcoRII CCWGG 2 cut(s) 280, 315
FaeI CATG 2 cut(s) 506, 560
FaiI YATR 6 cut(s) 77, 98, 504, 530, 558, 620
FalI AAGNNNNNCTT 4 cut(s) 723, 755, 750, 782
FaqI GGGAC 1 cut(s) 660
FatI CATG 2 cut(s) 502, 556
Fnu4HI GCNGC 4 cut(s) 109, 112, 445, 692
FokI GGATG 2 cut(s) 424, 485
Fsp4HI GCNGC 4 cut(s) 109, 112, 445, 692
FspAI RTGCGCAY 1 cut(s) 175
FspBI CTAG 1 cut(s) 781
FspI TGCGCA 1 cut(s) 175
GlaI GCGC 2 cut(s) 175, 355
GluI GCNGC 4 cut(s) 109, 112, 445, 692
GsaI CCCAGC 1 cut(s) 145
HapII CCGG 5 cut(s) 24, 38, 167, 214, 360
HgaI GACGC 3 cut(s) 103, 114, 515
HhaI GCGC 2 cut(s) 176, 356
Hin1I GRCGYC 2 cut(s) 114, 507
Hin1II CATG 2 cut(s) 506, 560
Hin6I GCGC 2 cut(s) 174, 354
HinP1I GCGC 2 cut(s) 174, 354
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HindIII AAGCTT 1 cut(s) 759
HinfI GANTC 2 cut(s) 310, 326
HpaII CCGG 5 cut(s) 24, 38, 167, 214, 360
HphI GGTGA 3 cut(s) 38, 316, 640
Hpy166II GTNNAC 1 cut(s) 247
Hpy188I TCNGA 2 cut(s) 69, 703
Hpy188III TCNNGA 4 cut(s) 84, 418, 557, 606
Hpy8I GTNNAC 1 cut(s) 247
HpyAV CCTTC 3 cut(s) 170, 578, 582
HpyCH4III ACNGT 2 cut(s) 642, 657
HpyCH4IV ACGT 2 cut(s) 243, 489
HpyF3I CTNAG 2 cut(s) 681, 751
HpySE526I ACGT 2 cut(s) 243, 489
Hsp92I GRCGYC 2 cut(s) 114, 507
Hsp92II CATG 2 cut(s) 506, 560
HspAI GCGC 2 cut(s) 174, 354
Kzo9I GATC 9 cut(s) 11, 42, 69, 189, 208, 336, 453, 532, 559
LmnI GCTCC 1 cut(s) 385
LweI GCATC 2 cut(s) 611, 667
MaeI CTAG 1 cut(s) 781
MaeII ACGT 2 cut(s) 243, 489
MaeIII GTNAC 1 cut(s) 485
MalI GATC 9 cut(s) 13, 44, 71, 191, 210, 338, 455, 534, 561
MboI GATC 9 cut(s) 11, 42, 69, 189, 208, 336, 453, 532, 559
MboII GAAGA 7 cut(s) 41, 64, 109, 233, 319, 569, 701
MflI RGATCY 4 cut(s) 11, 42, 69, 208
MluCI AATT 1 cut(s) 54
MmeI TCCRAC 2 cut(s) 474, 726
MnlI CCTC 7 cut(s) 203, 402, 421, 560, 602, 605, 718
MseI TTAA 2 cut(s) 543, 716
MslI CAYNNNNRTG 1 cut(s) 169
MspI CCGG 5 cut(s) 24, 38, 167, 214, 360
MspR9I CCNGG 3 cut(s) 24, 282, 317
Mva1269I GAATGC 1 cut(s) 176
MvaI CCWGG 2 cut(s) 282, 317
MvnI CGCG 2 cut(s) 108, 302
NciI CCSGG 1 cut(s) 24
NdeII GATC 9 cut(s) 11, 42, 69, 189, 208, 336, 453, 532, 559
NlaIII CATG 2 cut(s) 506, 560
NlaIV GGNNCC 1 cut(s) 348
NmeAIII GCCGAG 1 cut(s) 535
NmuCI GTSAC 1 cut(s) 485
NsbI TGCGCA 1 cut(s) 175
PagI TCATGA 1 cut(s) 556
PctI GAATGC 1 cut(s) 176
PfeI GAWTC 2 cut(s) 310, 326
PfoI TCCNGGA 1 cut(s) 280
PkrI GCNGC 4 cut(s) 110, 113, 446, 693
Psp6I CCWGG 2 cut(s) 280, 315
PspFI CCCAGC 1 cut(s) 141
PspGI CCWGG 2 cut(s) 280, 315
PspN4I GGNNCC 1 cut(s) 348
PspPI GGNCC 1 cut(s) 278
PsuI RGATCY 4 cut(s) 11, 42, 69, 208
RsaI GTAC 1 cut(s) 404
RsaNI GTAC 1 cut(s) 403
RseI CAYNNNNRTG 1 cut(s) 169
SaqAI TTAA 2 cut(s) 543, 716
SatI GCNGC 4 cut(s) 109, 112, 445, 692
Sau3AI GATC 9 cut(s) 11, 42, 69, 189, 208, 336, 453, 532, 559
Sau96I GGNCC 1 cut(s) 278
ScrFI CCNGG 3 cut(s) 24, 282, 317
SfaNI GCATC 2 cut(s) 611, 667
SinI GGWCC 1 cut(s) 278
SmiMI CAYNNNNRTG 1 cut(s) 169
SmlI CTYRAG 2 cut(s) 729, 756
SmoI CTYRAG 2 cut(s) 729, 756
Sse9I AATT 1 cut(s) 54
SsiI CCGC 9 cut(s) 108, 111, 196, 204, 302, 444, 447, 461, 692
SspMI CTAG 1 cut(s) 781
StyD4I CCNGG 3 cut(s) 22, 280, 315
TaaI ACNGT 2 cut(s) 642, 657
TaiI ACGT 2 cut(s) 246, 492
TaqI TCGA 2 cut(s) 188, 659
TasI AATT 1 cut(s) 54
TauI GCSGC 4 cut(s) 111, 114, 447, 694
TfiI GAWTC 2 cut(s) 310, 326
Tru1I TTAA 2 cut(s) 543, 716
Tru9I TTAA 2 cut(s) 543, 716
TseFI GTSAC 1 cut(s) 485
Tsp45I GTSAC 1 cut(s) 485
TspDTI ATGAA 1 cut(s) 64
TspGWI ACGGA 2 cut(s) 440, 542
VpaK11BI GGWCC 1 cut(s) 278
XcmI CCANNNNNNNNNTGG 1 cut(s) 589
XspI CTAG 1 cut(s) 781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.