pycom10g08260

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003321
Physical Location & Seq
Reverse (-)
10768549 .. 10770130
1582 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g08260.1

Sequence Viewer

Length: 870 bp
ATGACAAATACGGCTCATCTGCCCTCAGATTACAACGAACCAAACACCGATCCAGCCAGGATGGACTCCCTAACCCCCGACGCCAAACAGATCTTCATCTTATCCGGCCAGAGCAACATGGCCGGCCGCGGCGGCGTCTTCAGGGACCACCACCACCACAATCGCCAGCAATGGGACGGCGTCGTTCCGTCGGAGTGCCAGCCCCACCCTTCCATCCACCGCCTCGACGCACATCTCCGGTGGGTGCCCGCGCGTGAGCCGCTACACGCCGACATTGACGCCAAGAAGGTTTGCGGCGTGGGCCCGGGGATGGCGTTCGCGAACAAGGTGAGGGAGTGTGCGGGGGAGGTGGGGCTCGTGCCGTGCGCGGTGGGCGGGACGGCGATCAAGGAGTGGGCGCGTGGGGAGCACTTGTACGAGAGTATGGTGAAGCGGGCGAGGGAGAGCGTGAAAGGCGGGGGTGAGATGAAGGGGTTGTTGTGGTACCAAGGGGAGAGTGACACGTCGACGCAGCATGATGCGGATGCGTACCAGGGGAACATGGTGAAGCTAATTGAGAATGTGCGCCAGGATCTTGATTTGCCTTGGCTTCCAATTATCCAGGTAGCTATTGTGTCCGGGGACAATAAGTATATAGAGAAAGTGAGGGAGGCCCAATTGGGAATGGAAATCCCAAACGTCGTGTGCGTGGATGCCAAGGGATTGGAGCTCAAAGATGATCGATCTGGTGTCACCTCACCACCAAATCCCAGGTTCAGTTGGGTCACATGTTGGCTGATGCATACCTCCAGCATTTTGGACCCTCAGAACCCAATTCTCATGCTGCCCCTTGAAGTACACTATCTAATTTCACGGCGTTACTTCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

290

Amino Acids

32.3

Weight (kDa)

6.1

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 29 - 247 1.7e-87 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 483
AccB1I GGYRCC 2 cut(s) 244, 483
AccI GTMKAC 1 cut(s) 506
AccII CGCG 6 cut(s) 129, 251, 253, 320, 368, 400
AclWI GGATC 2 cut(s) 44, 579
AcoI YGGCCR 3 cut(s) 106, 120, 124
AcuI CTGAAG 1 cut(s) 124
AcyI GRCGYC 4 cut(s) 81, 135, 180, 279
AfaI GTAC 4 cut(s) 416, 485, 530, 837
AfiI CCNNNNNNNGG 2 cut(s) 172, 310
AflIII ACRYGT 2 cut(s) 501, 767
AgsI TTSAA 1 cut(s) 833
AjiI CACGTC 1 cut(s) 504
AjnI CCWGG 5 cut(s) 56, 531, 567, 600, 749
AjuI GAANNNNNNNTTGG 2 cut(s) 77, 109
AluBI AGCT 3 cut(s) 550, 608, 709
AluI AGCT 3 cut(s) 550, 608, 709
Alw21I GWGCWC 2 cut(s) 411, 711
AlwI GGATC 2 cut(s) 44, 579
Ama87I CYCGRG 1 cut(s) 304
AoxI GGCC 5 cut(s) 106, 120, 124, 301, 651
ApaI GGGCCC 1 cut(s) 305
ApeKI GCWGC 2 cut(s) 511, 823
Asp718I GGTACC 1 cut(s) 483
AspLEI GCGC 4 cut(s) 253, 368, 400, 567
AspS9I GGNCC 5 cut(s) 145, 301, 302, 652, 799
AsuC2I CCSGG 3 cut(s) 305, 306, 619
AsuHPI GGTGA 6 cut(s) 340, 439, 473, 556, 724, 729
AvaI CYCGRG 1 cut(s) 304
AvaII GGWCC 2 cut(s) 145, 799
BaeGI GKGCMC 2 cut(s) 249, 305
BanI GGYRCC 2 cut(s) 244, 483
BanII GRGCYC 3 cut(s) 305, 357, 711
BauI CACGAG 1 cut(s) 356
BbsI GAAGAC 1 cut(s) 130
Bbv12I GWGCWC 2 cut(s) 411, 711
BbvI GCAGC 2 cut(s) 523, 810
BccI CCATC 3 cut(s) 55, 221, 304
BceAI ACGGC 4 cut(s) 27, 193, 346, 396
BciT130I CCWGG 5 cut(s) 58, 533, 569, 602, 751
BcnI CCSGG 3 cut(s) 305, 306, 619
BglI GCCNNNNNGGC 1 cut(s) 132
BglII AGATCT 1 cut(s) 90
BisI GCNGC 7 cut(s) 127, 130, 133, 260, 295, 512, 824
BlsI GCNGC 7 cut(s) 128, 131, 134, 261, 296, 513, 825
Bme1390I CCNGG 8 cut(s) 58, 305, 306, 533, 569, 602, 619, 751
Bme18I GGWCC 2 cut(s) 145, 799
BmeT110I CYCGRG 1 cut(s) 304
BmgBI CACGTC 1 cut(s) 504
BmgT120I GGNCC 5 cut(s) 145, 301, 302, 652, 799
BmiI GGNNCC 5 cut(s) 146, 246, 303, 485, 801
BmrFI CCNGG 8 cut(s) 58, 305, 306, 533, 569, 602, 619, 751
BmsI GCATC 4 cut(s) 508, 514, 682, 768
BpiI GAAGAC 1 cut(s) 130
BpmI CTGGAG 1 cut(s) 772
BpuMI CCSGG 3 cut(s) 305, 306, 619
Bsa29I ATCGAT 1 cut(s) 721
BsaBI GATNNNNATC 1 cut(s) 95
BsaHI GRCGYC 4 cut(s) 81, 135, 180, 279
BsaJI CCNNGG 9 cut(s) 127, 304, 305, 487, 532, 584, 618, 696, 749
BsaWI WCCGGW 1 cut(s) 237
BsaXI ACNNNNNCTCC 2 cut(s) 398, 428
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 310
Bse118I RCCGGY 1 cut(s) 122
Bse3DI GCAATG 1 cut(s) 176
Bse8I GATNNNNATC 1 cut(s) 95
BseBI CCWGG 5 cut(s) 58, 533, 569, 602, 751
BseCI ATCGAT 1 cut(s) 721
BseDI CCNNGG 9 cut(s) 127, 304, 305, 487, 532, 584, 618, 696, 749
BseGI GGATG 5 cut(s) 66, 213, 315, 529, 697
BseJI GATNNNNATC 1 cut(s) 95
BseLI CCNNNNNNNGG 2 cut(s) 172, 310
BseMI GCAATG 1 cut(s) 176
BseMII CTCAG 2 cut(s) 39, 818
BseSI GKGCMC 2 cut(s) 249, 305
BseX3I CGGCCG 1 cut(s) 124
BseXI GCAGC 2 cut(s) 523, 810
Bsh1236I CGCG 6 cut(s) 129, 251, 253, 320, 368, 400
Bsh1285I CGRYCG 1 cut(s) 127
BshFI GGCC 5 cut(s) 108, 122, 126, 303, 653
BshNI GGYRCC 2 cut(s) 244, 483
BshVI ATCGAT 1 cut(s) 721
BsiEI CGRYCG 1 cut(s) 127
BsiHKAI GWGCWC 2 cut(s) 411, 711
BsiHKCI CYCGRG 1 cut(s) 304
BsiSI CCGG 5 cut(s) 105, 123, 238, 305, 618
BslFI GGGAC 4 cut(s) 158, 188, 391, 635
BslI CCNNNNNNNGG 2 cut(s) 172, 310
BsmFI GGGAC 4 cut(s) 158, 188, 391, 635
BsnI GGCC 5 cut(s) 108, 122, 126, 303, 653
BsoBI CYCGRG 1 cut(s) 304
Bsp120I GGGCCC 1 cut(s) 301
Bsp1286I GDGCHC 5 cut(s) 249, 305, 357, 411, 711
Bsp143I GATC 6 cut(s) 49, 90, 384, 571, 718, 722
Bsp68I TCGCGA 1 cut(s) 320
BspANI GGCC 5 cut(s) 108, 122, 126, 303, 653
BspCNI CTCAG 2 cut(s) 38, 817
BspDI ATCGAT 1 cut(s) 721
BspFNI CGCG 6 cut(s) 129, 251, 253, 320, 368, 400
BspLI GGNNCC 5 cut(s) 146, 246, 303, 485, 801
BspPI GGATC 2 cut(s) 44, 579
BspT107I GGYRCC 2 cut(s) 244, 483
BsrDI GCAATG 1 cut(s) 176
BsrFI RCCGGY 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 9 cut(s) 127, 304, 305, 487, 532, 584, 618, 696, 749
BssMI GATC 6 cut(s) 49, 90, 384, 571, 718, 722
BssNI GRCGYC 4 cut(s) 81, 135, 180, 279
BssSI CACGAG 1 cut(s) 356
BssT1I CCWWGG 3 cut(s) 487, 584, 696
Bst2BI CACGAG 1 cut(s) 356
Bst2UI CCWGG 5 cut(s) 58, 533, 569, 602, 751
BstACI GRCGYC 4 cut(s) 81, 135, 180, 279
BstC8I GCNNGC 5 cut(s) 124, 167, 200, 249, 435
BstDEI CTNAG 2 cut(s) 25, 804
BstDSI CCRYGG 1 cut(s) 127
BstF5I GGATG 5 cut(s) 66, 213, 315, 529, 697
BstFNI CGCG 6 cut(s) 129, 251, 253, 320, 368, 400
BstHHI GCGC 4 cut(s) 253, 368, 400, 567
BstKTI GATC 6 cut(s) 52, 93, 387, 574, 721, 725
BstMBI GATC 6 cut(s) 49, 90, 384, 571, 718, 722
BstMCI CGRYCG 1 cut(s) 127
BstMWI GCNNNNNNNGC 6 cut(s) 132, 259, 300, 372, 406, 453
BstNI CCWGG 5 cut(s) 58, 533, 569, 602, 751
BstNSI RCATGY 1 cut(s) 771
BstSCI CCNGG 8 cut(s) 56, 303, 304, 531, 567, 600, 617, 749
BstSLI GKGCMC 2 cut(s) 249, 305
BstUI CGCG 6 cut(s) 129, 251, 253, 320, 368, 400
BstV1I GCAGC 2 cut(s) 523, 810
BstV2I GAAGAC 1 cut(s) 130
BstX2I RGATCY 2 cut(s) 90, 571
BstXI CCANNNNNNTGG 2 cut(s) 703, 796
BstYI RGATCY 2 cut(s) 90, 571
BstZI CGGCCG 1 cut(s) 124
Bsu15I ATCGAT 1 cut(s) 721
BsuRI GGCC 5 cut(s) 108, 122, 126, 303, 653
BsuTUI ATCGAT 1 cut(s) 721
BtgI CCRYGG 1 cut(s) 127
BtrI CACGTC 1 cut(s) 504
BtsCI GGATG 5 cut(s) 66, 213, 315, 529, 697
BtuMI TCGCGA 1 cut(s) 320
Cac8I GCNNGC 5 cut(s) 124, 167, 200, 249, 435
CfoI GCGC 4 cut(s) 253, 368, 400, 567
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 5 cut(s) 145, 301, 302, 652, 799
Cfr42I CCGCGG 1 cut(s) 130
Cfr9I CCCGGG 1 cut(s) 304
ClaI ATCGAT 1 cut(s) 721
CseI GACGC 6 cut(s) 89, 124, 169, 236, 287, 517
Csp6I GTAC 4 cut(s) 415, 484, 529, 836
CviAII CATG 5 cut(s) 118, 515, 541, 768, 820
CviQI GTAC 4 cut(s) 415, 484, 529, 836
DdeI CTNAG 2 cut(s) 25, 804
DpnI GATC 6 cut(s) 51, 92, 386, 573, 720, 724
DpnII GATC 6 cut(s) 49, 90, 384, 571, 718, 722
EaeI YGGCCR 3 cut(s) 106, 120, 124
EagI CGGCCG 1 cut(s) 124
Ecl136II GAGCTC 1 cut(s) 709
EclXI CGGCCG 1 cut(s) 124
Eco130I CCWWGG 3 cut(s) 487, 584, 696
Eco24I GRGCYC 3 cut(s) 305, 357, 711
Eco47I GGWCC 2 cut(s) 145, 799
Eco52I CGGCCG 1 cut(s) 124
Eco53kI GAGCTC 1 cut(s) 709
Eco57I CTGAAG 1 cut(s) 124
Eco88I CYCGRG 1 cut(s) 304
EcoICRI GAGCTC 1 cut(s) 709
EcoRII CCWGG 5 cut(s) 56, 531, 567, 600, 749
EcoT14I CCWWGG 3 cut(s) 487, 584, 696
EcoT22I ATGCAT 1 cut(s) 783
EcoT38I GRGCYC 3 cut(s) 305, 357, 711
ErhI CCWWGG 3 cut(s) 487, 584, 696
FaeI CATG 5 cut(s) 121, 518, 544, 771, 823
FaiI YATR 9 cut(s) 119, 425, 516, 542, 633, 635, 769, 783, 821
FaqI GGGAC 4 cut(s) 158, 188, 391, 635
FatI CATG 5 cut(s) 117, 514, 540, 767, 819
FauI CCCGC 5 cut(s) 256, 334, 368, 426, 449
FblI GTMKAC 1 cut(s) 506
Fnu4HI GCNGC 7 cut(s) 127, 130, 133, 260, 295, 512, 824
FokI GGATG 5 cut(s) 73, 200, 322, 536, 704
FriOI GRGCYC 3 cut(s) 305, 357, 711
FseI GGCCGGCC 1 cut(s) 126
Fsp4HI GCNGC 7 cut(s) 127, 130, 133, 260, 295, 512, 824
GlaI GCGC 4 cut(s) 252, 367, 399, 566
GluI GCNGC 7 cut(s) 127, 130, 133, 260, 295, 512, 824
GsuI CTGGAG 1 cut(s) 772
HaeIII GGCC 5 cut(s) 108, 122, 126, 303, 653
HapII CCGG 5 cut(s) 105, 123, 238, 305, 618
HgaI GACGC 6 cut(s) 89, 124, 169, 236, 287, 517
HhaI GCGC 4 cut(s) 253, 368, 400, 567
Hin1I GRCGYC 4 cut(s) 81, 135, 180, 279
Hin1II CATG 5 cut(s) 121, 518, 544, 771, 823
Hin6I GCGC 4 cut(s) 251, 366, 398, 565
HinP1I GCGC 4 cut(s) 251, 366, 398, 565
HincII GTYRAC 1 cut(s) 507
HindII GTYRAC 1 cut(s) 507
HinfI GANTC 1 cut(s) 65
HpaII CCGG 5 cut(s) 105, 123, 238, 305, 618
HphI GGTGA 6 cut(s) 340, 439, 473, 556, 724, 729
Hpy166II GTNNAC 2 cut(s) 507, 838
Hpy188I TCNGA 3 cut(s) 28, 193, 807
Hpy188III TCNNGA 2 cut(s) 319, 575
Hpy8I GTNNAC 2 cut(s) 507, 838
Hpy99I CGWCG 7 cut(s) 83, 185, 193, 230, 508, 511, 683
HpyAV CCTTC 3 cut(s) 219, 280, 463
HpyCH4IV ACGT 2 cut(s) 503, 678
HpyCH4V TGCA 1 cut(s) 781
HpyF10VI GCNNNNNNNGC 6 cut(s) 132, 259, 300, 372, 406, 453
HpyF3I CTNAG 2 cut(s) 25, 804
HpySE526I ACGT 2 cut(s) 503, 678
Hsp92I GRCGYC 4 cut(s) 81, 135, 180, 279
Hsp92II CATG 5 cut(s) 121, 518, 544, 771, 823
HspAI GCGC 4 cut(s) 251, 366, 398, 565
KpnI GGTACC 1 cut(s) 487
KroI GCCGGC 1 cut(s) 122
KroNI GCCGGC 1 cut(s) 124
KspI CCGCGG 1 cut(s) 130
Kzo9I GATC 6 cut(s) 49, 90, 384, 571, 718, 722
LmnI GCTCC 2 cut(s) 406, 706
Lsp1109I GCAGC 2 cut(s) 523, 810
LweI GCATC 4 cut(s) 508, 514, 682, 768
MaeII ACGT 2 cut(s) 503, 678
MaeIII GTNAC 4 cut(s) 497, 730, 763, 857
MalI GATC 6 cut(s) 51, 92, 386, 573, 720, 724
MboI GATC 6 cut(s) 49, 90, 384, 571, 718, 722
MboII GAAGA 2 cut(s) 85, 130
MfeI CAATTG 1 cut(s) 656
MflI RGATCY 2 cut(s) 90, 571
MhlI GDGCHC 5 cut(s) 249, 305, 357, 411, 711
MluCI AATT 5 cut(s) 552, 594, 656, 813, 846
MlyI GAGTC 1 cut(s) 59
MmeI TCCRAC 1 cut(s) 171
Mph1103I ATGCAT 1 cut(s) 783
MroNI GCCGGC 1 cut(s) 122
MseI TTAA 1 cut(s) 868
MspA1I CMGCKG 1 cut(s) 129
MspI CCGG 5 cut(s) 105, 123, 238, 305, 618
MspR9I CCNGG 8 cut(s) 58, 305, 306, 533, 569, 602, 619, 751
MunI CAATTG 1 cut(s) 656
MvaI CCWGG 5 cut(s) 58, 533, 569, 602, 751
MvnI CGCG 6 cut(s) 129, 251, 253, 320, 368, 400
MwoI GCNNNNNNNGC 6 cut(s) 132, 259, 300, 372, 406, 453
NaeI GCCGGC 1 cut(s) 124
NciI CCSGG 3 cut(s) 305, 306, 619
NdeII GATC 6 cut(s) 49, 90, 384, 571, 718, 722
NgoMIV GCCGGC 1 cut(s) 122
NlaIII CATG 5 cut(s) 121, 518, 544, 771, 823
NlaIV GGNNCC 5 cut(s) 146, 246, 303, 485, 801
NmuCI GTSAC 3 cut(s) 497, 730, 763
NruI TCGCGA 1 cut(s) 320
NsiI ATGCAT 1 cut(s) 783
NspI RCATGY 1 cut(s) 771
PciI ACATGT 1 cut(s) 767
PcsI WCGNNNNNNNCGW 1 cut(s) 684
PdiI GCCGGC 1 cut(s) 124
PflFI GACNNNGTC 1 cut(s) 179
PkrI GCNGC 7 cut(s) 128, 131, 134, 261, 296, 513, 825
PleI GAGTC 1 cut(s) 59
PpsI GAGTC 1 cut(s) 59
PscI ACATGT 1 cut(s) 767
Psp124BI GAGCTC 1 cut(s) 711
Psp6I CCWGG 5 cut(s) 56, 531, 567, 600, 749
PspGI CCWGG 5 cut(s) 56, 531, 567, 600, 749
PspN4I GGNNCC 5 cut(s) 146, 246, 303, 485, 801
PspOMI GGGCCC 1 cut(s) 301
PspPI GGNCC 5 cut(s) 145, 301, 302, 652, 799
PsuI RGATCY 2 cut(s) 90, 571
PsyI GACNNNGTC 1 cut(s) 179
RigI GGCCGGCC 1 cut(s) 126
RruI TCGCGA 1 cut(s) 320
RsaI GTAC 4 cut(s) 416, 485, 530, 837
RsaNI GTAC 4 cut(s) 415, 484, 529, 836
SacI GAGCTC 1 cut(s) 711
SacII CCGCGG 1 cut(s) 130
SalI GTCGAC 1 cut(s) 505
SaqAI TTAA 1 cut(s) 868
SatI GCNGC 7 cut(s) 127, 130, 133, 260, 295, 512, 824
Sau3AI GATC 6 cut(s) 49, 90, 384, 571, 718, 722
Sau96I GGNCC 5 cut(s) 145, 301, 302, 652, 799
SchI GAGTC 1 cut(s) 59
ScrFI CCNGG 8 cut(s) 58, 305, 306, 533, 569, 602, 619, 751
SduI GDGCHC 5 cut(s) 249, 305, 357, 411, 711
SfaNI GCATC 4 cut(s) 508, 514, 682, 768
Sfr303I CCGCGG 1 cut(s) 130
SgrBI CCGCGG 1 cut(s) 130
SgrDI CGTCGACG 1 cut(s) 505
SinI GGWCC 2 cut(s) 145, 799
SmaI CCCGGG 1 cut(s) 306
Sse9I AATT 5 cut(s) 552, 594, 656, 813, 846
SstI GAGCTC 1 cut(s) 711
StyD4I CCNGG 8 cut(s) 56, 303, 304, 531, 567, 600, 617, 749
StyI CCWWGG 3 cut(s) 487, 584, 696
TaiI ACGT 2 cut(s) 506, 681
TaqI TCGA 3 cut(s) 225, 506, 721
TasI AATT 5 cut(s) 552, 594, 656, 813, 846
TatI WGTACW 1 cut(s) 835
TauI GCSGC 5 cut(s) 129, 132, 135, 262, 297
Tru1I TTAA 1 cut(s) 868
Tru9I TTAA 1 cut(s) 868
TseFI GTSAC 3 cut(s) 497, 730, 763
TseI GCWGC 2 cut(s) 511, 823
Tsp45I GTSAC 3 cut(s) 497, 730, 763
TspDTI ATGAA 2 cut(s) 85, 482
TspGWI ACGGA 1 cut(s) 177
TspMI CCCGGG 1 cut(s) 304
Tth111I GACNNNGTC 1 cut(s) 179
VpaK11BI GGWCC 2 cut(s) 145, 799
XceI RCATGY 1 cut(s) 771
XmaI CCCGGG 1 cut(s) 304
XmiI GTMKAC 1 cut(s) 506
Zsp2I ATGCAT 1 cut(s) 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.