Rh6AG075100

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0003321
Physical Location & Seq
Reverse (-)
10606577 .. 10613141
6565 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG075100.1

Sequence Viewer

Length: 1173 bp
ATGAACTCCGAAGTCCACCCAATTCGTATTCCATTTTCTACTCGTTTCCCTTCAAACCTTATGCGATATCTTCTCCAACGAATGAGAGACGAGGATGAAGAGCAGGCTCTTTTAACCCATTTGCTTACTCTCCTAGTTCACTATCTCAACGACAAAGAGGAAGAGGAGGAGGAGGAGGATTCTCAAGAACATGCCGGAGAACAACACCGACTCGCTAACCGGAAGCGGCGAAGAACTCGCCATCGCGGCACAACTTCACGAAGAAGACGAAACGACGGCGTTCCGCGCCAAGCCTACGCTGGGCCCCACTCGCTTTCGTTGACAAGCGACGCGGAGAGCCCATTTAAACCAGAGAACTCGCTCATCCCAAGAACAAAACCCGACACGTCGTTTATCCTCCTTCCGAAAAACCCGGAAATGGAGTCGCCGGAGCAGATCTTCATTCTTTCGGGGCAGAGCAACATGGCGGGTCGGGGCGGCGTGATCCGTGACCACGGCTACAACCACCACCAGCACTGGGATGGCGTCGTTCCGTCGGAGGCTCAGCCCGACCCTTCGATCCTCCGCCTCAACGCTCACCTCCACTGGGAGGCGGCGCGTGAGCCTCTCCACGCCGACATCGACGCCAAAAAGGTGTGCGGTTTGGGTCCGGGAATGTCGTTCGCCAACGCGGTGAGGGGGCGCGTGGAGGGGAAGATTGGGCTGGTGCCGTGCGCTGTTGGCGGCACGGCGATCAAGGAGTGGGCGCGTGGGGAGCATCTGTACGAGAACATGGTGAAGAGGGCGCGTGAGAGCGTGATGAACGGGGGAGAGATCAAAGGGTTGCTGTGGTACCAGGGGGAGAGCGACACGTCGTCTCAGCATGATGTTGATGCGTACCACGAGAACATGGTTAGGCTCATTGAGAATGTGCGCCATGACCTTGCTTTGCCTTCACTTCCAATAATCCAGGTGGCTATATGTTCTGGTGATGAGAAGTTCTTGGAGAAAATTAGGGAGGTTCAATTGGGGATGAAGGTTGAGAATGTTGTGTGTGTGGATGCCAAGGGATTGGAGCTGAAAGATGATCATCTTCACCTGACTACCAAGGCTCAAGTTCAGTTGGGTCAGATGCTTGCTGATGCTTACCTCAAGCATTTTGGATCAGCTAATTCCCATCATCCTGCCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

390

Amino Acids

44.02

Weight (kDa)

6.29

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 144 - 378 2.6e-95 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 831
AccB1I GGYRCC 2 cut(s) 706, 831
AccII CGCG 8 cut(s) 246, 286, 332, 598, 671, 684, 748, 787
AclWI GGATC 3 cut(s) 478, 553, 1150
AcyI GRCGYC 2 cut(s) 525, 624
AfaI GTAC 3 cut(s) 764, 833, 878
AfiI CCNNNNNNNGG 5 cut(s) 300, 418, 517, 586, 589
AflIII ACRYGT 2 cut(s) 384, 849
AgsI TTSAA 2 cut(s) 54, 1004
AhdI GACNNNNNGTC 1 cut(s) 853
AjiI CACGTC 2 cut(s) 387, 852
AjnI CCWGG 2 cut(s) 834, 948
AluBI AGCT 2 cut(s) 1057, 1148
AluI AGCT 2 cut(s) 1057, 1148
Alw26I GTCTC 2 cut(s) 81, 861
AlwI GGATC 3 cut(s) 478, 553, 1150
AoxI GGCC 1 cut(s) 302
ApaI GGGCCC 1 cut(s) 306
Asp718I GGTACC 1 cut(s) 831
AspLEI GCGC 7 cut(s) 288, 598, 684, 716, 748, 787, 915
AspS9I GGNCC 3 cut(s) 302, 303, 647
AsuC2I CCSGG 2 cut(s) 413, 651
AsuHPI GGTGA 5 cut(s) 569, 685, 787, 980, 1067
AvaII GGWCC 1 cut(s) 647
BaeGI GKGCMC 1 cut(s) 306
BanI GGYRCC 2 cut(s) 706, 831
BanII GRGCYC 2 cut(s) 306, 341
BauI CACGAG 1 cut(s) 881
BbsI GAAGAC 1 cut(s) 271
BccI CCATC 3 cut(s) 249, 515, 1164
BceAI ACGGC 4 cut(s) 292, 511, 694, 744
BciT130I CCWGG 2 cut(s) 836, 950
BclI TGATCA 1 cut(s) 1066
BcnI CCSGG 2 cut(s) 413, 651
BcoDI GTCTC 2 cut(s) 81, 861
BfaI CTAG 1 cut(s) 134
BglI GCCNNNNNGGC 1 cut(s) 246
BglII AGATCT 1 cut(s) 435
BisI GCNGC 5 cut(s) 227, 247, 478, 594, 724
BlpI GCTNAGC 1 cut(s) 543
BlsI GCNGC 5 cut(s) 228, 248, 479, 595, 725
Bme1390I CCNGG 4 cut(s) 413, 651, 836, 950
Bme18I GGWCC 1 cut(s) 647
BmeRI GACNNNNNGTC 1 cut(s) 853
BmgBI CACGTC 2 cut(s) 387, 852
BmgT120I GGNCC 3 cut(s) 302, 303, 647
BmiI GGNNCC 5 cut(s) 304, 305, 648, 708, 833
BmrFI CCNGG 4 cut(s) 413, 651, 836, 950
BmrI ACTGGG 2 cut(s) 526, 595
BmsI GCATC 5 cut(s) 766, 862, 1030, 1101, 1111
BmuI ACTGGG 2 cut(s) 526, 595
BpiI GAAGAC 1 cut(s) 271
Bpu1102I GCTNAGC 1 cut(s) 543
BpuEI CTTGAG 3 cut(s) 168, 1077, 1115
BpuMI CCSGG 2 cut(s) 413, 651
BsaBI GATNNNNATC 1 cut(s) 1068
BsaHI GRCGYC 2 cut(s) 525, 624
BsaJI CCNNGG 4 cut(s) 493, 835, 1044, 1086
BsaWI WCCGGW 1 cut(s) 219
BsaXI ACNNNNNCTCC 2 cut(s) 746, 776
Bsc4I CCNNNNNNNGG 5 cut(s) 300, 418, 517, 586, 589
Bse1I ACTGG 2 cut(s) 521, 590
Bse8I GATNNNNATC 1 cut(s) 1068
BseBI CCWGG 2 cut(s) 836, 950
BseDI CCNNGG 4 cut(s) 493, 835, 1044, 1086
BseGI GGATG 6 cut(s) 100, 363, 526, 1017, 1045, 1159
BseJI GATNNNNATC 1 cut(s) 1068
BseLI CCNNNNNNNGG 5 cut(s) 300, 418, 517, 586, 589
BseMII CTCAG 2 cut(s) 557, 872
BseNI ACTGG 2 cut(s) 521, 590
BseRI GAGGAG 4 cut(s) 179, 182, 185, 188
BseSI GKGCMC 1 cut(s) 306
BseYI CCCAGC 1 cut(s) 299
Bsh1236I CGCG 8 cut(s) 246, 286, 332, 598, 671, 684, 748, 787
BshFI GGCC 1 cut(s) 304
BshNI GGYRCC 2 cut(s) 706, 831
BsiSI CCGG 5 cut(s) 195, 220, 413, 428, 650
BslI CCNNNNNNNGG 5 cut(s) 300, 418, 517, 586, 589
BsmAI GTCTC 2 cut(s) 81, 861
BsmBI CGTCTC 2 cut(s) 81, 861
BsnI GGCC 1 cut(s) 304
Bsp120I GGGCCC 1 cut(s) 302
Bsp1286I GDGCHC 2 cut(s) 306, 341
Bsp143I GATC 7 cut(s) 435, 483, 558, 732, 813, 1066, 1142
Bsp1720I GCTNAGC 1 cut(s) 543
BspANI GGCC 1 cut(s) 304
BspCNI CTCAG 2 cut(s) 556, 871
BspFNI CGCG 8 cut(s) 246, 286, 332, 598, 671, 684, 748, 787
BspLI GGNNCC 5 cut(s) 304, 305, 648, 708, 833
BspPI GGATC 3 cut(s) 478, 553, 1150
BspQI GCTCTTC 1 cut(s) 93
BspT107I GGYRCC 2 cut(s) 706, 831
BsrI ACTGG 2 cut(s) 521, 590
BssECI CCNNGG 4 cut(s) 493, 835, 1044, 1086
BssMI GATC 7 cut(s) 435, 483, 558, 732, 813, 1066, 1142
BssNI GRCGYC 2 cut(s) 525, 624
BssSI CACGAG 1 cut(s) 881
BssT1I CCWWGG 2 cut(s) 1044, 1086
Bst2BI CACGAG 1 cut(s) 881
Bst2UI CCWGG 2 cut(s) 836, 950
Bst6I CTCTTC 3 cut(s) 93, 156, 773
BstACI GRCGYC 2 cut(s) 525, 624
BstC8I GCNNGC 2 cut(s) 105, 1116
BstDEI CTNAG 2 cut(s) 543, 858
BstDSI CCRYGG 1 cut(s) 493
BstF5I GGATG 6 cut(s) 100, 363, 526, 1017, 1045, 1159
BstFNI CGCG 8 cut(s) 246, 286, 332, 598, 671, 684, 748, 787
BstHHI GCGC 7 cut(s) 288, 598, 684, 716, 748, 787, 915
BstKTI GATC 7 cut(s) 438, 486, 561, 735, 816, 1069, 1145
BstMAI GTCTC 2 cut(s) 81, 861
BstMBI GATC 7 cut(s) 435, 483, 558, 732, 813, 1066, 1142
BstMWI GCNNNNNNNGC 5 cut(s) 246, 285, 310, 720, 754
BstNI CCWGG 2 cut(s) 836, 950
BstNSI RCATGY 1 cut(s) 194
BstSCI CCNGG 4 cut(s) 411, 649, 834, 948
BstSLI GKGCMC 1 cut(s) 306
BstUI CGCG 8 cut(s) 246, 286, 332, 598, 671, 684, 748, 787
BstV2I GAAGAC 1 cut(s) 271
BstX2I RGATCY 1 cut(s) 435
BstXI CCANNNNNNTGG 1 cut(s) 1051
BstYI RGATCY 1 cut(s) 435
BsuRI GGCC 1 cut(s) 304
BtgI CCRYGG 1 cut(s) 493
BtgZI GCGATG 1 cut(s) 227
BtrI CACGTC 2 cut(s) 387, 852
BtsCI GGATG 6 cut(s) 100, 363, 526, 1017, 1045, 1159
BtsIMutI CAGTG 2 cut(s) 514, 583
Cac8I GCNNGC 2 cut(s) 105, 1116
CfoI GCGC 7 cut(s) 288, 598, 684, 716, 748, 787, 915
Cfr13I GGNCC 3 cut(s) 302, 303, 647
CseI GACGC 3 cut(s) 338, 514, 632
Csp6I GTAC 3 cut(s) 763, 832, 877
CviAII CATG 6 cut(s) 191, 463, 772, 863, 889, 917
CviQI GTAC 3 cut(s) 763, 832, 877
DdeI CTNAG 2 cut(s) 543, 858
DpnI GATC 7 cut(s) 437, 485, 560, 734, 815, 1068, 1144
DpnII GATC 7 cut(s) 435, 483, 558, 732, 813, 1066, 1142
DraI TTTAAA 1 cut(s) 346
DriI GACNNNNNGTC 1 cut(s) 853
Eam1104I CTCTTC 3 cut(s) 93, 156, 773
Eam1105I GACNNNNNGTC 1 cut(s) 853
EarI CTCTTC 3 cut(s) 93, 156, 773
EciI GGCGGA 1 cut(s) 554
Eco130I CCWWGG 2 cut(s) 1044, 1086
Eco24I GRGCYC 2 cut(s) 306, 341
Eco32I GATATC 1 cut(s) 68
Eco47I GGWCC 1 cut(s) 647
EcoO109I RGGNCCY 1 cut(s) 303
EcoRII CCWGG 2 cut(s) 834, 948
EcoRV GATATC 1 cut(s) 68
EcoT14I CCWWGG 2 cut(s) 1044, 1086
EcoT38I GRGCYC 2 cut(s) 306, 341
ErhI CCWWGG 2 cut(s) 1044, 1086
Esp3I CGTCTC 2 cut(s) 81, 861
FaeI CATG 6 cut(s) 194, 466, 775, 866, 892, 920
FaiI YATR 9 cut(s) 62, 192, 464, 773, 864, 890, 918, 959, 961
FatI CATG 6 cut(s) 190, 462, 771, 862, 888, 916
FauI CCCGC 1 cut(s) 460
FbaI TGATCA 1 cut(s) 1066
Fnu4HI GCNGC 5 cut(s) 227, 247, 478, 594, 724
FokI GGATG 6 cut(s) 107, 350, 533, 1024, 1052, 1146
FriOI GRGCYC 2 cut(s) 306, 341
Fsp4HI GCNGC 5 cut(s) 227, 247, 478, 594, 724
FspBI CTAG 1 cut(s) 134
GlaI GCGC 7 cut(s) 287, 597, 683, 715, 747, 786, 914
GluI GCNGC 5 cut(s) 227, 247, 478, 594, 724
GsaI CCCAGC 1 cut(s) 303
HaeIII GGCC 1 cut(s) 304
HapII CCGG 5 cut(s) 195, 220, 413, 428, 650
HgaI GACGC 3 cut(s) 338, 514, 632
HhaI GCGC 7 cut(s) 288, 598, 684, 716, 748, 787, 915
Hin1I GRCGYC 2 cut(s) 525, 624
Hin1II CATG 6 cut(s) 194, 466, 775, 866, 892, 920
Hin6I GCGC 7 cut(s) 286, 596, 682, 714, 746, 785, 913
HinP1I GCGC 7 cut(s) 286, 596, 682, 714, 746, 785, 913
HincII GTYRAC 1 cut(s) 321
HindII GTYRAC 1 cut(s) 321
HinfI GANTC 3 cut(s) 179, 210, 422
HpaII CCGG 5 cut(s) 195, 220, 413, 428, 650
HphI GGTGA 5 cut(s) 569, 685, 787, 980, 1067
Hpy166II GTNNAC 3 cut(s) 16, 139, 321
Hpy188I TCNGA 4 cut(s) 10, 405, 538, 1110
Hpy188III TCNNGA 2 cut(s) 185, 258
Hpy8I GTNNAC 3 cut(s) 16, 139, 321
Hpy99I CGWCG 7 cut(s) 278, 332, 391, 530, 538, 626, 856
HpyAV CCTTC 5 cut(s) 60, 410, 564, 942, 1009
HpyCH4IV ACGT 2 cut(s) 386, 851
HpyF10VI GCNNNNNNNGC 5 cut(s) 246, 285, 310, 720, 754
HpyF3I CTNAG 2 cut(s) 543, 858
HpySE526I ACGT 2 cut(s) 386, 851
Hsp92I GRCGYC 2 cut(s) 525, 624
Hsp92II CATG 6 cut(s) 194, 466, 775, 866, 892, 920
HspAI GCGC 7 cut(s) 286, 596, 682, 714, 746, 785, 913
KpnI GGTACC 1 cut(s) 835
Ksp22I TGATCA 1 cut(s) 1066
Kzo9I GATC 7 cut(s) 435, 483, 558, 732, 813, 1066, 1142
LguI GCTCTTC 1 cut(s) 93
LmnI GCTCC 3 cut(s) 430, 754, 1054
LweI GCATC 5 cut(s) 766, 862, 1030, 1101, 1111
MaeI CTAG 1 cut(s) 134
MaeII ACGT 2 cut(s) 386, 851
MaeIII GTNAC 1 cut(s) 488
MalI GATC 7 cut(s) 437, 485, 560, 734, 815, 1068, 1144
MboI GATC 7 cut(s) 435, 483, 558, 732, 813, 1066, 1142
MfeI CAATTG 1 cut(s) 1004
MflI RGATCY 1 cut(s) 435
MhlI GDGCHC 2 cut(s) 306, 341
MluCI AATT 4 cut(s) 21, 990, 1004, 1150
MlyI GAGTC 2 cut(s) 204, 431
MmeI TCCRAC 2 cut(s) 100, 516
MseI TTAA 2 cut(s) 113, 345
MslI CAYNNNNRTG 1 cut(s) 519
MspI CCGG 5 cut(s) 195, 220, 413, 428, 650
MspR9I CCNGG 4 cut(s) 413, 651, 836, 950
MunI CAATTG 1 cut(s) 1004
MvaI CCWGG 2 cut(s) 836, 950
MvnI CGCG 8 cut(s) 246, 286, 332, 598, 671, 684, 748, 787
MwoI GCNNNNNNNGC 5 cut(s) 246, 285, 310, 720, 754
NciI CCSGG 2 cut(s) 413, 651
NdeII GATC 7 cut(s) 435, 483, 558, 732, 813, 1066, 1142
NlaIII CATG 6 cut(s) 194, 466, 775, 866, 892, 920
NlaIV GGNNCC 5 cut(s) 304, 305, 648, 708, 833
NmuCI GTSAC 1 cut(s) 488
NspI RCATGY 1 cut(s) 194
PciSI GCTCTTC 1 cut(s) 93
PcsI WCGNNNNNNNCGW 2 cut(s) 265, 618
PfeI GAWTC 1 cut(s) 179
PfoI TCCNGGA 1 cut(s) 649
PkrI GCNGC 5 cut(s) 228, 248, 479, 595, 725
PleI GAGTC 2 cut(s) 204, 430
PpsI GAGTC 2 cut(s) 204, 430
Psp6I CCWGG 2 cut(s) 834, 948
PspFI CCCAGC 1 cut(s) 299
PspGI CCWGG 2 cut(s) 834, 948
PspN4I GGNNCC 5 cut(s) 304, 305, 648, 708, 833
PspOMI GGGCCC 1 cut(s) 302
PspPI GGNCC 3 cut(s) 302, 303, 647
PsuI RGATCY 1 cut(s) 435
RsaI GTAC 3 cut(s) 764, 833, 878
RsaNI GTAC 3 cut(s) 763, 832, 877
RseI CAYNNNNRTG 1 cut(s) 519
SapI GCTCTTC 1 cut(s) 93
SaqAI TTAA 2 cut(s) 113, 345
SatI GCNGC 5 cut(s) 227, 247, 478, 594, 724
Sau3AI GATC 7 cut(s) 435, 483, 558, 732, 813, 1066, 1142
Sau96I GGNCC 3 cut(s) 302, 303, 647
SchI GAGTC 2 cut(s) 204, 431
ScrFI CCNGG 4 cut(s) 413, 651, 836, 950
SduI GDGCHC 2 cut(s) 306, 341
SfaNI GCATC 5 cut(s) 766, 862, 1030, 1101, 1111
SinI GGWCC 1 cut(s) 647
SmiMI CAYNNNNRTG 1 cut(s) 519
SmlI CTYRAG 3 cut(s) 183, 1092, 1130
SmoI CTYRAG 3 cut(s) 183, 1092, 1130
Sse9I AATT 4 cut(s) 21, 990, 1004, 1150
SspMI CTAG 1 cut(s) 134
StyD4I CCNGG 4 cut(s) 411, 649, 834, 948
StyI CCWWGG 2 cut(s) 1044, 1086
TaiI ACGT 2 cut(s) 389, 854
TaqI TCGA 2 cut(s) 557, 621
TasI AATT 4 cut(s) 21, 990, 1004, 1150
TauI GCSGC 5 cut(s) 229, 249, 480, 596, 726
TfiI GAWTC 1 cut(s) 179
Tru1I TTAA 2 cut(s) 113, 345
Tru9I TTAA 2 cut(s) 113, 345
TscAI CASTG 2 cut(s) 521, 590
TseFI GTSAC 1 cut(s) 488
Tsp45I GTSAC 1 cut(s) 488
TspDTI ATGAA 5 cut(s) 17, 111, 430, 815, 1028
TspGWI ACGGA 2 cut(s) 476, 522
TspRI CASTG 2 cut(s) 521, 590
VpaK11BI GGWCC 1 cut(s) 647
XceI RCATGY 1 cut(s) 194
XcmI CCANNNNNNNNNTGG 2 cut(s) 296, 518
XspI CTAG 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.