Rh6DG064800

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0003321
Physical Location & Seq
Forward (+)
6651060 .. 6652752
1693 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG064800.1

Sequence Viewer

Length: 1158 bp
ATGAACTCCGAACTCGACCCAACTCGTAATCCATTCTCTTCTCGTTTTCCTCGTTCAAGGCTTATGCGACTTTGTCTGGATGATATGAATGCCTCTGCTCAAAGGCGGGCTCTGTTAATGCGCGGCATGTTTACTCTCTTAGCTCACTATCTCGACGACGACGACGACGAAGACGAAGAGGATCCCCAAGAACAAGCCGGAGAACGAGACCCACCAGCTAACCGGAAGCGGCGCCGCACAACTACTCGAAAGGAAAACGACGACGTTCCGCCCAAAGCCTACGCTGGGCCCCACTCGTCTTCGTTGACAAGCGACGCGGTGAGCCCATTTAAACCCGAGAAGTCGCTCATTCCAAGGACAAAACCCGACACGTCGTTTATCCTCCACCCGAAATACCCGGAAATGGAGTCGCCGGAGCAGATCTTCATTCTTTCGGGTCAGAGCAACATGGCGGGTAGGGGCGGCGTGATCCGCGACCACAGCCACAACCACCACCAGCACTGGGACGGCGTCGTTCCGTCGGAGGCTCAGCCCGATCCTTCGATCCTCCGCCTCAACGCTCACCTACACTGGGAGCCCGCGCGTGAGCCTCTCCACGCCGACATCGACGCCAAGAAGGTGTGCGGTTTGGGTCCGGGAATGGCGTTCGCCAACGCGGTGAGGGAGCGCGTGGAGGGGAAGATTGGGCTGGTGCCGTGCGCGGTTGGCGGCACGGCGATAAAGGAGTGGGCGCTTGGGGAGCATCTATACGAGAACATGGTGAAGAGGGCGCGTGAGAGCGTGAAGAACGGGGGCGAGATCAAAGGGTTGCTGTGGTACCAGGGGGAGAGCGACACGTCGTCTGAGCATGATGTTGATGCGTACCACGAGAACATGGTTAGGCTCATTGAGAATGTGCGCCAGGACCTTGCTTTGCCTTCACTTCCAATAATCCAGGTGGCTATTTGTTCTGGTGATGAGAAGTACTTGGAGAAAATTAGGGAGGTTCAATTGGGAATGAAGGTTGAGAATGTGGTGTGTGTGGATGCTAAGGGATTAAAGCTGAAAGATGATCATCTTCACCTGACCACTGAGGCTCAAGTTCAGTTGGGTCAGATGCTGGCTGATGCTTACCTCAAGCATTTTGGATCAGCTAATTCCCATCATCCTGCCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

385

Amino Acids

43.11

Weight (kDa)

5.88

Isoelectric Point (pI)

40.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 139 - 373 6.9e-96 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 816
AccB1I GGYRCC 3 cut(s) 231, 691, 816
AccII CGCG 9 cut(s) 123, 317, 474, 581, 583, 656, 669, 701, 772
AclWI GGATC 6 cut(s) 176, 189, 463, 530, 538, 1135
AcyI GRCGYC 3 cut(s) 232, 510, 609
AfaI GTAC 3 cut(s) 818, 863, 965
AfiI CCNNNNNNNGG 4 cut(s) 285, 403, 502, 571
AflIII ACRYGT 2 cut(s) 369, 834
AgsI TTSAA 2 cut(s) 57, 989
AhdI GACNNNNNGTC 1 cut(s) 838
AjiI CACGTC 2 cut(s) 372, 837
AjnI CCWGG 3 cut(s) 819, 900, 933
AluBI AGCT 4 cut(s) 143, 218, 1042, 1133
AluI AGCT 4 cut(s) 143, 218, 1042, 1133
Alw26I GTCTC 1 cut(s) 201
AlwI GGATC 6 cut(s) 176, 189, 463, 530, 538, 1135
AlwNI CAGNNNCTG 1 cut(s) 1099
Ama87I CYCGRG 1 cut(s) 335
AoxI GGCC 1 cut(s) 287
ApaI GGGCCC 1 cut(s) 291
Asp718I GGTACC 1 cut(s) 816
AspLEI GCGC 8 cut(s) 123, 234, 583, 669, 701, 733, 772, 900
AspS9I GGNCC 4 cut(s) 287, 288, 632, 904
AsuC2I CCSGG 2 cut(s) 398, 636
AsuHPI GGTGA 6 cut(s) 331, 554, 670, 772, 965, 1052
AvaI CYCGRG 1 cut(s) 335
AvaII GGWCC 2 cut(s) 632, 904
BaeGI GKGCMC 1 cut(s) 291
BamHI GGATCC 1 cut(s) 181
BanI GGYRCC 3 cut(s) 231, 691, 816
BanII GRGCYC 4 cut(s) 112, 291, 326, 579
BauI CACGAG 1 cut(s) 866
BbsI GAAGAC 2 cut(s) 177, 291
BccI CCATC 1 cut(s) 1149
BceAI ACGGC 3 cut(s) 523, 679, 729
BciT130I CCWGG 3 cut(s) 821, 902, 935
BclI TGATCA 1 cut(s) 1051
BcnI CCSGG 2 cut(s) 398, 636
BcoDI GTCTC 1 cut(s) 201
BfoI RGCGCY 2 cut(s) 235, 734
BglII AGATCT 1 cut(s) 420
BisI GCNGC 5 cut(s) 124, 230, 235, 463, 709
BlpI GCTNAGC 1 cut(s) 528
BlsI GCNGC 5 cut(s) 125, 231, 236, 464, 710
BmcAI AGTACT 1 cut(s) 965
Bme1390I CCNGG 5 cut(s) 398, 636, 821, 902, 935
Bme18I GGWCC 2 cut(s) 632, 904
BmeRI GACNNNNNGTC 1 cut(s) 838
BmeT110I CYCGRG 1 cut(s) 335
BmgBI CACGTC 2 cut(s) 372, 837
BmgT120I GGNCC 4 cut(s) 287, 288, 632, 904
BmiI GGNNCC 8 cut(s) 183, 233, 289, 290, 576, 633, 693, 818
BmrFI CCNGG 5 cut(s) 398, 636, 821, 902, 935
BmrI ACTGGG 2 cut(s) 511, 580
BmsI GCATC 5 cut(s) 751, 847, 1015, 1086, 1096
BmuI ACTGGG 2 cut(s) 511, 580
BpiI GAAGAC 2 cut(s) 177, 291
Bpu10I CCTNAGC 1 cut(s) 1029
Bpu1102I GCTNAGC 1 cut(s) 528
BpuEI CTTGAG 2 cut(s) 1062, 1100
BpuMI CCSGG 2 cut(s) 398, 636
BsaBI GATNNNNATC 1 cut(s) 1053
BsaHI GRCGYC 3 cut(s) 232, 510, 609
BsaI GGTCTC 1 cut(s) 201
BsaJI CCNNGG 2 cut(s) 353, 820
BsaWI WCCGGW 1 cut(s) 222
Bsc4I CCNNNNNNNGG 4 cut(s) 285, 403, 502, 571
Bse1I ACTGG 2 cut(s) 506, 575
Bse8I GATNNNNATC 1 cut(s) 1053
BseBI CCWGG 3 cut(s) 821, 902, 935
BseDI CCNNGG 2 cut(s) 353, 820
BseGI GGATG 3 cut(s) 85, 1030, 1144
BseJI GATNNNNATC 1 cut(s) 1053
BseLI CCNNNNNNNGG 4 cut(s) 285, 403, 502, 571
BseMII CTCAG 3 cut(s) 542, 834, 1062
BseNI ACTGG 2 cut(s) 506, 575
BseSI GKGCMC 1 cut(s) 291
BseYI CCCAGC 1 cut(s) 284
Bsh1236I CGCG 9 cut(s) 123, 317, 474, 581, 583, 656, 669, 701, 772
BshFI GGCC 1 cut(s) 289
BshNI GGYRCC 3 cut(s) 231, 691, 816
BsiHKCI CYCGRG 1 cut(s) 335
BsiSI CCGG 5 cut(s) 198, 223, 398, 413, 635
BslFI GGGAC 1 cut(s) 518
BslI CCNNNNNNNGG 4 cut(s) 285, 403, 502, 571
BsmAI GTCTC 1 cut(s) 201
BsmFI GGGAC 1 cut(s) 518
BsmI GAATGC 1 cut(s) 94
BsnI GGCC 1 cut(s) 289
Bso31I GGTCTC 1 cut(s) 201
BsoBI CYCGRG 1 cut(s) 335
Bsp120I GGGCCC 1 cut(s) 287
Bsp1286I GDGCHC 4 cut(s) 112, 291, 326, 579
Bsp143I GATC 8 cut(s) 181, 420, 468, 535, 543, 798, 1051, 1127
Bsp1720I GCTNAGC 1 cut(s) 528
BspANI GGCC 1 cut(s) 289
BspCNI CTCAG 3 cut(s) 541, 835, 1063
BspFNI CGCG 9 cut(s) 123, 317, 474, 581, 583, 656, 669, 701, 772
BspLI GGNNCC 8 cut(s) 183, 233, 289, 290, 576, 633, 693, 818
BspPI GGATC 6 cut(s) 176, 189, 463, 530, 538, 1135
BspT107I GGYRCC 3 cut(s) 231, 691, 816
BspTNI GGTCTC 1 cut(s) 201
BsrI ACTGG 2 cut(s) 506, 575
BssECI CCNNGG 2 cut(s) 353, 820
BssMI GATC 8 cut(s) 181, 420, 468, 535, 543, 798, 1051, 1127
BssNI GRCGYC 3 cut(s) 232, 510, 609
BssSI CACGAG 1 cut(s) 866
BssT1I CCWWGG 1 cut(s) 353
Bst2BI CACGAG 1 cut(s) 866
Bst2UI CCWGG 3 cut(s) 821, 902, 935
Bst6I CTCTTC 3 cut(s) 43, 171, 758
BstACI GRCGYC 3 cut(s) 232, 510, 609
BstC8I GCNNGC 3 cut(s) 108, 579, 1101
BstDEI CTNAG 5 cut(s) 139, 528, 843, 1029, 1071
BstF5I GGATG 3 cut(s) 85, 1030, 1144
BstFNI CGCG 9 cut(s) 123, 317, 474, 581, 583, 656, 669, 701, 772
BstH2I RGCGCY 2 cut(s) 235, 734
BstHHI GCGC 8 cut(s) 123, 234, 583, 669, 701, 733, 772, 900
BstKTI GATC 8 cut(s) 184, 423, 471, 538, 546, 801, 1054, 1130
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 8 cut(s) 181, 420, 468, 535, 543, 798, 1051, 1127
BstMWI GCNNNNNNNGC 4 cut(s) 471, 480, 705, 739
BstNI CCWGG 3 cut(s) 821, 902, 935
BstNSI RCATGY 1 cut(s) 130
BstSCI CCNGG 5 cut(s) 396, 634, 819, 900, 933
BstSLI GKGCMC 1 cut(s) 291
BstUI CGCG 9 cut(s) 123, 317, 474, 581, 583, 656, 669, 701, 772
BstV2I GAAGAC 2 cut(s) 177, 291
BstX2I RGATCY 2 cut(s) 181, 420
BstYI RGATCY 2 cut(s) 181, 420
BsuRI GGCC 1 cut(s) 289
BtrI CACGTC 2 cut(s) 372, 837
BtsCI GGATG 3 cut(s) 85, 1030, 1144
BtsIMutI CAGTG 3 cut(s) 499, 568, 1068
Cac8I GCNNGC 3 cut(s) 108, 579, 1101
CaiI CAGNNNCTG 1 cut(s) 1099
CfoI GCGC 8 cut(s) 123, 234, 583, 669, 701, 733, 772, 900
Cfr13I GGNCC 4 cut(s) 287, 288, 632, 904
CseI GACGC 3 cut(s) 323, 499, 617
Csp6I GTAC 3 cut(s) 817, 862, 964
CviAII CATG 5 cut(s) 127, 448, 757, 848, 874
CviQI GTAC 3 cut(s) 817, 862, 964
DdeI CTNAG 5 cut(s) 139, 528, 843, 1029, 1071
DinI GGCGCC 1 cut(s) 233
DpnI GATC 8 cut(s) 183, 422, 470, 537, 545, 800, 1053, 1129
DpnII GATC 8 cut(s) 181, 420, 468, 535, 543, 798, 1051, 1127
DraI TTTAAA 1 cut(s) 331
DriI GACNNNNNGTC 1 cut(s) 838
Eam1104I CTCTTC 3 cut(s) 43, 171, 758
Eam1105I GACNNNNNGTC 1 cut(s) 838
EarI CTCTTC 3 cut(s) 43, 171, 758
EciI GGCGGA 2 cut(s) 258, 539
Eco130I CCWWGG 1 cut(s) 353
Eco24I GRGCYC 4 cut(s) 112, 291, 326, 579
Eco31I GGTCTC 1 cut(s) 201
Eco47I GGWCC 2 cut(s) 632, 904
Eco88I CYCGRG 1 cut(s) 335
EcoO109I RGGNCCY 2 cut(s) 288, 904
EcoRII CCWGG 3 cut(s) 819, 900, 933
EcoT14I CCWWGG 1 cut(s) 353
EcoT38I GRGCYC 4 cut(s) 112, 291, 326, 579
EgeI GGCGCC 1 cut(s) 233
EheI GGCGCC 1 cut(s) 233
ErhI CCWWGG 1 cut(s) 353
FaeI CATG 5 cut(s) 130, 451, 760, 851, 877
FaiI YATR 8 cut(s) 65, 86, 128, 449, 748, 758, 849, 875
FaqI GGGAC 1 cut(s) 518
FatI CATG 5 cut(s) 126, 447, 756, 847, 873
FauI CCCGC 3 cut(s) 99, 445, 586
FbaI TGATCA 1 cut(s) 1051
Fnu4HI GCNGC 5 cut(s) 124, 230, 235, 463, 709
FokI GGATG 3 cut(s) 92, 1037, 1131
FriOI GRGCYC 4 cut(s) 112, 291, 326, 579
Fsp4HI GCNGC 5 cut(s) 124, 230, 235, 463, 709
GlaI GCGC 8 cut(s) 122, 233, 582, 668, 700, 732, 771, 899
GluI GCNGC 5 cut(s) 124, 230, 235, 463, 709
GsaI CCCAGC 1 cut(s) 288
HaeII RGCGCY 2 cut(s) 235, 734
HaeIII GGCC 1 cut(s) 289
HapII CCGG 5 cut(s) 198, 223, 398, 413, 635
HgaI GACGC 3 cut(s) 323, 499, 617
HhaI GCGC 8 cut(s) 123, 234, 583, 669, 701, 733, 772, 900
Hin1I GRCGYC 3 cut(s) 232, 510, 609
Hin1II CATG 5 cut(s) 130, 451, 760, 851, 877
Hin6I GCGC 8 cut(s) 121, 232, 581, 667, 699, 731, 770, 898
HinP1I GCGC 8 cut(s) 121, 232, 581, 667, 699, 731, 770, 898
HincII GTYRAC 1 cut(s) 306
HindII GTYRAC 1 cut(s) 306
HinfI GANTC 1 cut(s) 407
HpaII CCGG 5 cut(s) 198, 223, 398, 413, 635
HphI GGTGA 6 cut(s) 331, 554, 670, 772, 965, 1052
Hpy166II GTNNAC 2 cut(s) 132, 306
Hpy188I TCNGA 5 cut(s) 10, 441, 523, 844, 1095
Hpy188III TCNNGA 2 cut(s) 77, 152
Hpy8I GTNNAC 2 cut(s) 132, 306
HpyAV CCTTC 4 cut(s) 549, 610, 927, 994
HpyCH4IV ACGT 3 cut(s) 264, 371, 836
HpyF10VI GCNNNNNNNGC 4 cut(s) 471, 480, 705, 739
HpyF3I CTNAG 5 cut(s) 139, 528, 843, 1029, 1071
HpySE526I ACGT 3 cut(s) 264, 371, 836
Hsp92I GRCGYC 3 cut(s) 232, 510, 609
Hsp92II CATG 5 cut(s) 130, 451, 760, 851, 877
HspAI GCGC 8 cut(s) 121, 232, 581, 667, 699, 731, 770, 898
KasI GGCGCC 1 cut(s) 231
KpnI GGTACC 1 cut(s) 820
Ksp22I TGATCA 1 cut(s) 1051
Kzo9I GATC 8 cut(s) 181, 420, 468, 535, 543, 798, 1051, 1127
LmnI GCTCC 4 cut(s) 415, 574, 664, 739
LweI GCATC 5 cut(s) 751, 847, 1015, 1086, 1096
MaeII ACGT 3 cut(s) 264, 371, 836
MalI GATC 8 cut(s) 183, 422, 470, 537, 545, 800, 1053, 1129
MboI GATC 8 cut(s) 181, 420, 468, 535, 543, 798, 1051, 1127
MboII GAAGA 9 cut(s) 30, 182, 188, 291, 415, 691, 775, 796, 1049
MfeI CAATTG 1 cut(s) 989
MflI RGATCY 2 cut(s) 181, 420
MhlI GDGCHC 4 cut(s) 112, 291, 326, 579
MluCI AATT 3 cut(s) 975, 989, 1135
Mly113I GGCGCC 1 cut(s) 232
MlyI GAGTC 1 cut(s) 416
MmeI TCCRAC 1 cut(s) 501
MseI TTAA 4 cut(s) 116, 330, 1037, 1156
MspI CCGG 5 cut(s) 198, 223, 398, 413, 635
MspR9I CCNGG 5 cut(s) 398, 636, 821, 902, 935
MunI CAATTG 1 cut(s) 989
Mva1269I GAATGC 1 cut(s) 94
MvaI CCWGG 3 cut(s) 821, 902, 935
MvnI CGCG 9 cut(s) 123, 317, 474, 581, 583, 656, 669, 701, 772
MwoI GCNNNNNNNGC 4 cut(s) 471, 480, 705, 739
NarI GGCGCC 1 cut(s) 232
NciI CCSGG 2 cut(s) 398, 636
NdeII GATC 8 cut(s) 181, 420, 468, 535, 543, 798, 1051, 1127
NlaIII CATG 5 cut(s) 130, 451, 760, 851, 877
NlaIV GGNNCC 8 cut(s) 183, 233, 289, 290, 576, 633, 693, 818
NspI RCATGY 1 cut(s) 130
PcsI WCGNNNNNNNCGW 6 cut(s) 49, 159, 162, 165, 171, 603
PctI GAATGC 1 cut(s) 94
PflFI GACNNNGTC 2 cut(s) 72, 509
PfoI TCCNGGA 1 cut(s) 634
PkrI GCNGC 5 cut(s) 125, 231, 236, 464, 710
PleI GAGTC 1 cut(s) 415
PluTI GGCGCC 1 cut(s) 235
PpsI GAGTC 1 cut(s) 415
PpuMI RGGWCCY 1 cut(s) 904
Psp5II RGGWCCY 1 cut(s) 904
Psp6I CCWGG 3 cut(s) 819, 900, 933
PspFI CCCAGC 1 cut(s) 284
PspGI CCWGG 3 cut(s) 819, 900, 933
PspN4I GGNNCC 8 cut(s) 183, 233, 289, 290, 576, 633, 693, 818
PspOMI GGGCCC 1 cut(s) 287
PspPI GGNCC 4 cut(s) 287, 288, 632, 904
PspPPI RGGWCCY 1 cut(s) 904
PstNI CAGNNNCTG 1 cut(s) 1099
PsuI RGATCY 2 cut(s) 181, 420
PsyI GACNNNGTC 2 cut(s) 72, 509
RsaI GTAC 3 cut(s) 818, 863, 965
RsaNI GTAC 3 cut(s) 817, 862, 964
SaqAI TTAA 4 cut(s) 116, 330, 1037, 1156
SatI GCNGC 5 cut(s) 124, 230, 235, 463, 709
Sau3AI GATC 8 cut(s) 181, 420, 468, 535, 543, 798, 1051, 1127
Sau96I GGNCC 4 cut(s) 287, 288, 632, 904
ScaI AGTACT 1 cut(s) 965
SchI GAGTC 1 cut(s) 416
ScrFI CCNGG 5 cut(s) 398, 636, 821, 902, 935
SduI GDGCHC 4 cut(s) 112, 291, 326, 579
SfaNI GCATC 5 cut(s) 751, 847, 1015, 1086, 1096
SfoI GGCGCC 1 cut(s) 233
SinI GGWCC 2 cut(s) 632, 904
SmlI CTYRAG 2 cut(s) 1077, 1115
SmoI CTYRAG 2 cut(s) 1077, 1115
Sse9I AATT 3 cut(s) 975, 989, 1135
SspDI GGCGCC 1 cut(s) 231
StyD4I CCNGG 5 cut(s) 396, 634, 819, 900, 933
StyI CCWWGG 1 cut(s) 353
TaiI ACGT 3 cut(s) 267, 374, 839
TaqI TCGA 5 cut(s) 15, 153, 247, 542, 606
TasI AATT 3 cut(s) 975, 989, 1135
TatI WGTACW 1 cut(s) 963
TauI GCSGC 5 cut(s) 126, 232, 237, 465, 711
Tru1I TTAA 4 cut(s) 116, 330, 1037, 1156
Tru9I TTAA 4 cut(s) 116, 330, 1037, 1156
TscAI CASTG 3 cut(s) 506, 575, 1075
TspDTI ATGAA 4 cut(s) 17, 101, 415, 1013
TspGWI ACGGA 1 cut(s) 507
TspRI CASTG 3 cut(s) 506, 575, 1075
Tth111I GACNNNGTC 2 cut(s) 72, 509
VpaK11BI GGWCC 2 cut(s) 632, 904
XceI RCATGY 1 cut(s) 130
ZrmI AGTACT 1 cut(s) 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.