Rorug05G0558500

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003321
Physical Location & Seq
Reverse (-)
74761674 .. 74763510
1837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0558500.1

Sequence Viewer

Length: 747 bp
ATGGCAGCTGCTATTCGATCTATGCTCGGAAAGTCAGGAAATAGAATATCCTCAGTAGCAAACATGGTGACTATGGGGGATTCTGAAATTAGAACCCTAAATATTAATCATAATCTAAATTTGGGTTACTCCTCTCGTCACCCTCTTGAATCTTTTTCGGTAGTAAACCGTCCCAGCAATATTCGAAGGCCTAAGAAGCTGGAACCTAAACACCCGGAAATTCCAAGAAAGTTATATGGAGCAAGTCTTTTCTCTCGCTCCTCCAGATATTGTACCAGGGTTGTACTTTATTCACATCTCGCACTGGAAGAATTCAACAAGCAAAAGAATGGCCAACTGCAATTTGTAAAAGTGGTGAAGGCATTTAGCCAGTTGAGTGGTGGTTGGAAGAATCATTTGCTAACATTGGAGGCAATTGATGCTGATAATGTGATCAAAACTTACCAAGCTATAGTTGTGGAACATTTTCCTAGCAACCCACCTCCGCAGCTCGTATTATTTGGCTTGGTTGCTGATAACGGCAATGGGAGTCTGTTAAAGCTTATTGACAACAGGCCTGCATGCAGGGGGGGACCGGTCGATAATCGATATGTTGAGCGAGAGAACATAAAGCTTCAAAAGAAGACGAAGTATTCTCTGGAGGATGGGATACTGAGCAGCTTGCAGTCTAGCACATGGTTACTCAAGAGGTTTGATCTAGAAAAAAAAAACATGCATGAAAAGCTCATCAAGTATAGCACATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

248

Amino Acids

28.05

Weight (kDa)

9.92

Isoelectric Point (pI)

50.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQAPI PF16845 94 - 155 4.1e-10 Aspartic acid proteinase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 745
AciI CCGC 1 cut(s) 485
AcoI YGGCCR 1 cut(s) 331
AcsI RAATTY 3 cut(s) 118, 219, 311
AfaI GTAC 2 cut(s) 274, 285
AgeI ACCGGT 1 cut(s) 574
AgsI TTSAA 3 cut(s) 149, 316, 617
AjnI CCWGG 1 cut(s) 275
AluBI AGCT 8 cut(s) 8, 199, 449, 490, 541, 613, 660, 724
AluI AGCT 8 cut(s) 8, 199, 449, 490, 541, 613, 660, 724
AoxI GGCC 3 cut(s) 188, 331, 554
ApeKI GCWGC 4 cut(s) 5, 8, 487, 657
ApoI RAATTY 3 cut(s) 118, 219, 311
AseI ATTAAT 1 cut(s) 105
AsiGI ACCGGT 1 cut(s) 574
Asp700I GAANNNNTTC 1 cut(s) 465
AspS9I GGNCC 1 cut(s) 572
AsuC2I CCSGG 1 cut(s) 215
AsuHPI GGTGA 3 cut(s) 79, 131, 367
AsuII TTCGAA 1 cut(s) 184
AvaII GGWCC 1 cut(s) 572
BalI TGGCCA 1 cut(s) 333
BbsI GAAGAC 1 cut(s) 629
BbvI GCAGC 3 cut(s) 17, 499, 669
BccI CCATC 1 cut(s) 638
BceAI ACGGC 1 cut(s) 535
BciT130I CCWGG 1 cut(s) 277
BciVI GTATCC 1 cut(s) 642
BclI TGATCA 1 cut(s) 432
BcnI CCSGG 1 cut(s) 215
BfaI CTAG 3 cut(s) 471, 669, 698
BfmI CTRYAG 1 cut(s) 450
BfuI GTATCC 1 cut(s) 642
BisI GCNGC 4 cut(s) 6, 9, 488, 658
BlsI GCNGC 4 cut(s) 7, 10, 489, 659
Bme1390I CCNGG 2 cut(s) 215, 277
Bme18I GGWCC 1 cut(s) 572
BmgT120I GGNCC 1 cut(s) 572
BmiI GGNNCC 2 cut(s) 204, 573
BmrFI CCNGG 2 cut(s) 215, 277
BmsI GCATC 1 cut(s) 409
BpiI GAAGAC 1 cut(s) 629
BpmI CTGGAG 2 cut(s) 247, 659
Bpu14I TTCGAA 1 cut(s) 184
BpuEI CTTGAG 1 cut(s) 668
BpuMI CCSGG 1 cut(s) 215
Bsa29I ATCGAT 1 cut(s) 586
BsaJI CCNNGG 1 cut(s) 276
BsaWI WCCGGW 1 cut(s) 574
Bse118I RCCGGY 1 cut(s) 574
Bse1I ACTGG 2 cut(s) 309, 370
Bse3DI GCAATG 1 cut(s) 529
BseBI CCWGG 1 cut(s) 277
BseCI ATCGAT 1 cut(s) 586
BseDI CCNNGG 1 cut(s) 276
BseGI GGATG 1 cut(s) 649
BseMI GCAATG 1 cut(s) 529
BseMII CTCAG 2 cut(s) 66, 644
BseNI ACTGG 2 cut(s) 309, 370
BseRI GAGGAG 2 cut(s) 121, 250
BseXI GCAGC 3 cut(s) 17, 499, 669
BseYI CCCAGC 1 cut(s) 173
Bsh1285I CGRYCG 1 cut(s) 579
BshFI GGCC 3 cut(s) 190, 333, 556
BshTI ACCGGT 1 cut(s) 574
BshVI ATCGAT 1 cut(s) 586
BsiEI CGRYCG 1 cut(s) 579
BsiSI CCGG 2 cut(s) 215, 575
BslFI GGGAC 2 cut(s) 156, 585
BsmFI GGGAC 2 cut(s) 156, 585
BsnI GGCC 3 cut(s) 190, 333, 556
Bsp119I TTCGAA 1 cut(s) 184
Bsp143I GATC 3 cut(s) 17, 432, 694
BspACI CCGC 1 cut(s) 485
BspANI GGCC 3 cut(s) 190, 333, 556
BspCNI CTCAG 2 cut(s) 65, 645
BspDI ATCGAT 1 cut(s) 586
BspLI GGNNCC 2 cut(s) 204, 573
BspT104I TTCGAA 1 cut(s) 184
BsrDI GCAATG 1 cut(s) 529
BsrFI RCCGGY 1 cut(s) 574
BsrI ACTGG 2 cut(s) 309, 370
BssAI RCCGGY 1 cut(s) 574
BssECI CCNNGG 1 cut(s) 276
BssMI GATC 3 cut(s) 17, 432, 694
Bst2UI CCWGG 1 cut(s) 277
Bst4CI ACNGT 1 cut(s) 170
BstAPI GCANNNNNTGC 1 cut(s) 419
BstBI TTCGAA 1 cut(s) 184
BstC8I GCNNGC 3 cut(s) 558, 562, 662
BstDEI CTNAG 3 cut(s) 52, 192, 653
BstF5I GGATG 1 cut(s) 649
BstKTI GATC 3 cut(s) 20, 435, 697
BstMBI GATC 3 cut(s) 17, 432, 694
BstMCI CGRYCG 1 cut(s) 579
BstMWI GCNNNNNNNGC 3 cut(s) 196, 419, 721
BstNI CCWGG 1 cut(s) 277
BstNSI RCATGY 2 cut(s) 564, 715
BstSCI CCNGG 2 cut(s) 213, 275
BstSFI CTRYAG 1 cut(s) 450
BstV1I GCAGC 3 cut(s) 17, 499, 669
BstV2I GAAGAC 1 cut(s) 629
BstXI CCANNNNNNTGG 1 cut(s) 377
Bsu15I ATCGAT 1 cut(s) 586
BsuI GTATCC 1 cut(s) 642
BsuRI GGCC 3 cut(s) 190, 333, 556
BsuTUI ATCGAT 1 cut(s) 586
BtsCI GGATG 1 cut(s) 649
BtsIMutI CAGTG 1 cut(s) 302
Cac8I GCNNGC 3 cut(s) 558, 562, 662
Cfr10I RCCGGY 1 cut(s) 574
Cfr13I GGNCC 1 cut(s) 572
ClaI ATCGAT 1 cut(s) 586
Csp6I GTAC 2 cut(s) 273, 284
CspAI ACCGGT 1 cut(s) 574
CviAII CATG 5 cut(s) 64, 561, 675, 712, 716
CviQI GTAC 2 cut(s) 273, 284
DdeI CTNAG 3 cut(s) 52, 192, 653
DpnI GATC 3 cut(s) 19, 434, 696
DpnII GATC 3 cut(s) 17, 432, 694
EaeI YGGCCR 1 cut(s) 331
Eco147I AGGCCT 2 cut(s) 190, 556
Eco47I GGWCC 1 cut(s) 572
EcoRI GAATTC 1 cut(s) 311
EcoRII CCWGG 1 cut(s) 275
EcoT22I ATGCAT 1 cut(s) 717
FaeI CATG 5 cut(s) 67, 564, 678, 715, 719
FaqI GGGAC 2 cut(s) 156, 585
FatI CATG 5 cut(s) 63, 560, 674, 711, 715
FbaI TGATCA 1 cut(s) 432
Fnu4HI GCNGC 4 cut(s) 6, 9, 488, 658
FokI GGATG 1 cut(s) 656
Fsp4HI GCNGC 4 cut(s) 6, 9, 488, 658
FspBI CTAG 3 cut(s) 471, 669, 698
GluI GCNGC 4 cut(s) 6, 9, 488, 658
GsaI CCCAGC 1 cut(s) 177
GsuI CTGGAG 2 cut(s) 247, 659
HaeIII GGCC 3 cut(s) 190, 333, 556
HapII CCGG 2 cut(s) 215, 575
Hin1II CATG 5 cut(s) 67, 564, 678, 715, 719
HindIII AAGCTT 2 cut(s) 539, 611
HinfI GANTC 4 cut(s) 80, 149, 391, 529
HpaII CCGG 2 cut(s) 215, 575
HphI GGTGA 3 cut(s) 79, 131, 367
Hpy166II GTNNAC 1 cut(s) 166
Hpy188I TCNGA 2 cut(s) 29, 85
Hpy188III TCNNGA 6 cut(s) 36, 146, 264, 638, 685, 698
Hpy8I GTNNAC 1 cut(s) 166
HpyAV CCTTC 2 cut(s) 180, 352
HpyCH4III ACNGT 1 cut(s) 170
HpyCH4V TGCA 5 cut(s) 340, 560, 564, 664, 715
HpyF10VI GCNNNNNNNGC 3 cut(s) 196, 419, 721
HpyF3I CTNAG 3 cut(s) 52, 192, 653
Hsp92II CATG 5 cut(s) 67, 564, 678, 715, 719
Ksp22I TGATCA 1 cut(s) 432
Kzo9I GATC 3 cut(s) 17, 432, 694
LmnI GCTCC 2 cut(s) 239, 263
Lsp1109I GCAGC 3 cut(s) 17, 499, 669
LweI GCATC 1 cut(s) 409
MaeI CTAG 3 cut(s) 471, 669, 698
MaeIII GTNAC 4 cut(s) 67, 125, 137, 678
MalI GATC 3 cut(s) 19, 434, 696
MboI GATC 3 cut(s) 17, 432, 694
MboII GAAGA 3 cut(s) 320, 400, 634
MfeI CAATTG 1 cut(s) 414
MlsI TGGCCA 1 cut(s) 333
MluCI AATT 6 cut(s) 87, 118, 219, 311, 341, 414
MluNI TGGCCA 1 cut(s) 333
MlyI GAGTC 1 cut(s) 538
MmeI TCCRAC 1 cut(s) 365
MnlI CCTC 8 cut(s) 61, 142, 153, 271, 403, 492, 634, 681
Mox20I TGGCCA 1 cut(s) 333
Mph1103I ATGCAT 1 cut(s) 717
MroXI GAANNNNTTC 1 cut(s) 465
MscI TGGCCA 1 cut(s) 333
MseI TTAA 2 cut(s) 105, 536
Msp20I TGGCCA 1 cut(s) 333
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 2 cut(s) 215, 575
MspR9I CCNGG 2 cut(s) 215, 277
MunI CAATTG 1 cut(s) 414
MvaI CCWGG 1 cut(s) 277
MwoI GCNNNNNNNGC 3 cut(s) 196, 419, 721
NciI CCSGG 1 cut(s) 215
NdeII GATC 3 cut(s) 17, 432, 694
NlaIII CATG 5 cut(s) 67, 564, 678, 715, 719
NlaIV GGNNCC 2 cut(s) 204, 573
NmuCI GTSAC 2 cut(s) 67, 137
NsiI ATGCAT 1 cut(s) 717
NspI RCATGY 2 cut(s) 564, 715
NspV TTCGAA 1 cut(s) 184
PaeI GCATGC 1 cut(s) 564
PceI AGGCCT 2 cut(s) 190, 556
PdmI GAANNNNTTC 1 cut(s) 465
PfeI GAWTC 3 cut(s) 80, 149, 391
PinAI ACCGGT 1 cut(s) 574
PkrI GCNGC 4 cut(s) 7, 10, 489, 659
PleI GAGTC 1 cut(s) 537
PpsI GAGTC 1 cut(s) 537
PshBI ATTAAT 1 cut(s) 105
PsiI TTATAA 1 cut(s) 745
Psp6I CCWGG 1 cut(s) 275
PspFI CCCAGC 1 cut(s) 173
PspGI CCWGG 1 cut(s) 275
PspN4I GGNNCC 2 cut(s) 204, 573
PspPI GGNCC 1 cut(s) 572
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 2 cut(s) 274, 285
RsaNI GTAC 2 cut(s) 273, 284
SaqAI TTAA 2 cut(s) 105, 536
SatI GCNGC 4 cut(s) 6, 9, 488, 658
Sau3AI GATC 3 cut(s) 17, 432, 694
Sau96I GGNCC 1 cut(s) 572
SchI GAGTC 1 cut(s) 538
ScrFI CCNGG 2 cut(s) 215, 277
SfaNI GCATC 1 cut(s) 409
SfcI CTRYAG 1 cut(s) 450
SfuI TTCGAA 1 cut(s) 184
SinI GGWCC 1 cut(s) 572
SmlI CTYRAG 1 cut(s) 683
SmoI CTYRAG 1 cut(s) 683
SphI GCATGC 1 cut(s) 564
Sse9I AATT 6 cut(s) 87, 118, 219, 311, 341, 414
SseBI AGGCCT 2 cut(s) 190, 556
SsiI CCGC 1 cut(s) 485
SspI AATATT 2 cut(s) 103, 181
SspMI CTAG 3 cut(s) 471, 669, 698
StuI AGGCCT 2 cut(s) 190, 556
StyD4I CCNGG 2 cut(s) 213, 275
TaaI ACNGT 1 cut(s) 170
TaqI TCGA 4 cut(s) 16, 184, 579, 586
TasI AATT 6 cut(s) 87, 118, 219, 311, 341, 414
TatI WGTACW 1 cut(s) 283
TfiI GAWTC 3 cut(s) 80, 149, 391
Tru1I TTAA 2 cut(s) 105, 536
Tru9I TTAA 2 cut(s) 105, 536
TscAI CASTG 1 cut(s) 309
TseFI GTSAC 2 cut(s) 67, 137
TseI GCWGC 4 cut(s) 5, 8, 487, 657
Tsp45I GTSAC 2 cut(s) 67, 137
TspDTI ATGAA 1 cut(s) 732
TspRI CASTG 1 cut(s) 309
VpaK11BI GGWCC 1 cut(s) 572
VspI ATTAAT 1 cut(s) 105
XapI RAATTY 3 cut(s) 118, 219, 311
XbaI TCTAGA 1 cut(s) 697
XceI RCATGY 2 cut(s) 564, 715
XcmI CCANNNNNNNNNTGG 1 cut(s) 377
XmnI GAANNNNTTC 1 cut(s) 465
XspI CTAG 3 cut(s) 471, 669, 698
Zsp2I ATGCAT 1 cut(s) 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.