Rw6G006630

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0003321
Physical Location & Seq
Forward (+)
10682095 .. 10683825
1731 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G006630.1

Sequence Viewer

Length: 1182 bp
ATGAACTCCGAAGTCGACCCAATTCGTATTCCATTTTCTACTCGTTTCCCTTCAAACCTTATGCGATATCTTCTCCAACGAATGAGAGACGAGGATGAAGAGCAGGCTCTTTTAACCCATTTGCTTACTCTCCTAGTTCACTATCTCAACGACAAAGAGGAAGAGGAAGAGGAGGAGGAGGAGGAGGATTCTCAAGAGCATGCCGGAGAACAACACCGACTCGCTAACCGGAAGCGGCGAAGAACTCGCCGTCGCGGCACAACTACACGAAGAAGACGAAACGACGGCGTTCCGGGCCAAGCATACGCTGGGCCCCACTCGCCTTCGTTGACAAGCGACGCGGAGAGCCCATTTAAACCCGAGAACTCGCTCATTCCAAGAACAAAACCCGACACGTCGTTTATCCTCCATCCGAAAACCCCGGAAATGGAATCGCCGGAGCAGATCTTCATTCTTTCGGGGCAGAGCAACATGGCGGGTCGGGGCGGCGTGATCCGTGACCACGGCCACAACCACCACCAGCACTGGGACGGCGTCGTTCCGTCGGAGGCTCAGCCCGACCCTTCGATCCTCCGCCTCAACGCTCACCTCCGCTGGGAGGCGGCGCGTGAGCCTCTCCACGCCGACATCGACGCCAAGAAGGTGTGCGGTTTGGGTCCGGGAATGTCGTTCGCCAACGCGGTGAGGGGGCGCGTGGAGGGGAAGATTGGGCTGGTGCCGTGCGCTGTTGGCGGCACGGCGATCAAGGAGTGGGCGCGTGGGGAGCATCTGTACGAGAACATGGTGAAGAGGGCGCGTGAGAGCGTGAAGAACGGGGGAGAGATCAAAGGGTTGCTGTGGTACCAGGGGGAGAGCGACACGTCGTCTCAGCATGATGTTGATGCGTACCACGAGAACATGGTTAGGCTCATTGAGAATGTGCGCCATGACCTTGCTTTGCCTTCACTTCCAATTATCCAGGTGGCTATTTGTTCTGGTGATGAGAAGTTCTTGGAGAAAATTAGGGAGGTTCAATTGGGGATGAAGGTTGAGAATGTTGTGTGTGTGGATGCCAAGGGATTGGAGCTGAAGGATGATCATCTTCACCTGACTACCAAGGCACAAGTTCAGTTGGGTCAGATGCTTGCTGATGCTTACCTCAAGCATTTTGGATCAGCTAATTCCCATCATCCTGCCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

393

Amino Acids

44.31

Weight (kDa)

6.13

Isoelectric Point (pI)

51.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 147 - 381 8e-96 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 840
AccB1I GGYRCC 2 cut(s) 715, 840
AccI GTMKAC 1 cut(s) 15
AccII CGCG 7 cut(s) 255, 341, 607, 680, 693, 757, 796
AclWI GGATC 3 cut(s) 487, 562, 1159
AcoI YGGCCR 1 cut(s) 505
AcuI CTGAAG 1 cut(s) 1088
AcyI GRCGYC 2 cut(s) 534, 633
AfaI GTAC 3 cut(s) 773, 842, 887
AfiI CCNNNNNNNGG 4 cut(s) 427, 526, 595, 598
AflIII ACRYGT 2 cut(s) 393, 858
AgsI TTSAA 2 cut(s) 54, 1013
AhdI GACNNNNNGTC 1 cut(s) 862
AjiI CACGTC 2 cut(s) 396, 861
AjnI CCWGG 2 cut(s) 843, 957
AluBI AGCT 2 cut(s) 1066, 1157
AluI AGCT 2 cut(s) 1066, 1157
Alw26I GTCTC 2 cut(s) 81, 870
AlwI GGATC 3 cut(s) 487, 562, 1159
Ama87I CYCGRG 1 cut(s) 359
AoxI GGCC 3 cut(s) 295, 311, 505
ApaI GGGCCC 1 cut(s) 315
Asp718I GGTACC 1 cut(s) 840
AspLEI GCGC 6 cut(s) 607, 693, 725, 757, 796, 924
AspS9I GGNCC 4 cut(s) 295, 311, 312, 656
AsuC2I CCSGG 3 cut(s) 294, 422, 660
AsuHPI GGTGA 5 cut(s) 578, 694, 796, 989, 1076
AvaI CYCGRG 1 cut(s) 359
AvaII GGWCC 1 cut(s) 656
BaeGI GKGCMC 1 cut(s) 315
BanI GGYRCC 2 cut(s) 715, 840
BanII GRGCYC 2 cut(s) 315, 350
BauI CACGAG 1 cut(s) 890
BbsI GAAGAC 1 cut(s) 280
BccI CCATC 2 cut(s) 417, 1173
BceAI ACGGC 6 cut(s) 234, 301, 520, 547, 703, 753
BciT130I CCWGG 2 cut(s) 845, 959
BclI TGATCA 1 cut(s) 1075
BcnI CCSGG 3 cut(s) 294, 422, 660
BcoDI GTCTC 2 cut(s) 81, 870
BfaI CTAG 1 cut(s) 134
BglI GCCNNNNNGGC 1 cut(s) 255
BglII AGATCT 1 cut(s) 444
BisI GCNGC 5 cut(s) 236, 256, 487, 603, 733
BlpI GCTNAGC 1 cut(s) 552
BlsI GCNGC 5 cut(s) 237, 257, 488, 604, 734
Bme1390I CCNGG 5 cut(s) 294, 422, 660, 845, 959
Bme18I GGWCC 1 cut(s) 656
BmeRI GACNNNNNGTC 1 cut(s) 862
BmeT110I CYCGRG 1 cut(s) 359
BmgBI CACGTC 2 cut(s) 396, 861
BmgT120I GGNCC 4 cut(s) 295, 311, 312, 656
BmiI GGNNCC 5 cut(s) 313, 314, 657, 717, 842
BmrFI CCNGG 5 cut(s) 294, 422, 660, 845, 959
BmrI ACTGGG 1 cut(s) 535
BmsI GCATC 5 cut(s) 775, 871, 1039, 1110, 1120
BmuI ACTGGG 1 cut(s) 535
BpiI GAAGAC 1 cut(s) 280
Bpu1102I GCTNAGC 1 cut(s) 552
BpuEI CTTGAG 2 cut(s) 177, 1124
BpuMI CCSGG 3 cut(s) 294, 422, 660
BsaBI GATNNNNATC 1 cut(s) 1077
BsaHI GRCGYC 2 cut(s) 534, 633
BsaJI CCNNGG 5 cut(s) 420, 502, 844, 1053, 1095
BsaWI WCCGGW 1 cut(s) 228
BsaXI ACNNNNNCTCC 2 cut(s) 755, 785
Bsc4I CCNNNNNNNGG 4 cut(s) 427, 526, 595, 598
Bse1I ACTGG 1 cut(s) 530
Bse8I GATNNNNATC 1 cut(s) 1077
BseBI CCWGG 2 cut(s) 845, 959
BseDI CCNNGG 5 cut(s) 420, 502, 844, 1053, 1095
BseGI GGATG 6 cut(s) 100, 409, 1026, 1054, 1078, 1168
BseJI GATNNNNATC 1 cut(s) 1077
BseLI CCNNNNNNNGG 4 cut(s) 427, 526, 595, 598
BseMII CTCAG 2 cut(s) 566, 881
BseNI ACTGG 1 cut(s) 530
BseRI GAGGAG 5 cut(s) 185, 188, 191, 194, 197
BseSI GKGCMC 1 cut(s) 315
BseYI CCCAGC 2 cut(s) 308, 594
Bsh1236I CGCG 7 cut(s) 255, 341, 607, 680, 693, 757, 796
BshFI GGCC 3 cut(s) 297, 313, 507
BshNI GGYRCC 2 cut(s) 715, 840
BsiHKCI CYCGRG 1 cut(s) 359
BsiSI CCGG 6 cut(s) 204, 229, 293, 422, 437, 659
BslFI GGGAC 1 cut(s) 542
BslI CCNNNNNNNGG 4 cut(s) 427, 526, 595, 598
BsmAI GTCTC 2 cut(s) 81, 870
BsmBI CGTCTC 2 cut(s) 81, 870
BsmFI GGGAC 1 cut(s) 542
BsnI GGCC 3 cut(s) 297, 313, 507
BsoBI CYCGRG 1 cut(s) 359
Bsp120I GGGCCC 1 cut(s) 311
Bsp1286I GDGCHC 2 cut(s) 315, 350
Bsp143I GATC 7 cut(s) 444, 492, 567, 741, 822, 1075, 1151
Bsp1720I GCTNAGC 1 cut(s) 552
BspANI GGCC 3 cut(s) 297, 313, 507
BspCNI CTCAG 2 cut(s) 565, 880
BspFNI CGCG 7 cut(s) 255, 341, 607, 680, 693, 757, 796
BspLI GGNNCC 5 cut(s) 313, 314, 657, 717, 842
BspPI GGATC 3 cut(s) 487, 562, 1159
BspQI GCTCTTC 1 cut(s) 93
BspT107I GGYRCC 2 cut(s) 715, 840
BsrI ACTGG 1 cut(s) 530
BssECI CCNNGG 5 cut(s) 420, 502, 844, 1053, 1095
BssMI GATC 7 cut(s) 444, 492, 567, 741, 822, 1075, 1151
BssNI GRCGYC 2 cut(s) 534, 633
BssSI CACGAG 1 cut(s) 890
BssT1I CCWWGG 2 cut(s) 1053, 1095
Bst2BI CACGAG 1 cut(s) 890
Bst2UI CCWGG 2 cut(s) 845, 959
Bst6I CTCTTC 4 cut(s) 93, 156, 162, 782
BstACI GRCGYC 2 cut(s) 534, 633
BstC8I GCNNGC 3 cut(s) 105, 201, 1125
BstDEI CTNAG 2 cut(s) 552, 867
BstDSI CCRYGG 1 cut(s) 502
BstF5I GGATG 6 cut(s) 100, 409, 1026, 1054, 1078, 1168
BstFNI CGCG 7 cut(s) 255, 341, 607, 680, 693, 757, 796
BstHHI GCGC 6 cut(s) 607, 693, 725, 757, 796, 924
BstKTI GATC 7 cut(s) 447, 495, 570, 744, 825, 1078, 1154
BstMAI GTCTC 2 cut(s) 81, 870
BstMBI GATC 7 cut(s) 444, 492, 567, 741, 822, 1075, 1151
BstMWI GCNNNNNNNGC 5 cut(s) 255, 294, 319, 729, 763
BstNI CCWGG 2 cut(s) 845, 959
BstNSI RCATGY 1 cut(s) 203
BstSCI CCNGG 5 cut(s) 292, 420, 658, 843, 957
BstSLI GKGCMC 1 cut(s) 315
BstUI CGCG 7 cut(s) 255, 341, 607, 680, 693, 757, 796
BstV2I GAAGAC 1 cut(s) 280
BstX2I RGATCY 1 cut(s) 444
BstXI CCANNNNNNTGG 1 cut(s) 1060
BstYI RGATCY 1 cut(s) 444
BsuRI GGCC 3 cut(s) 297, 313, 507
BtgI CCRYGG 1 cut(s) 502
BtrI CACGTC 2 cut(s) 396, 861
BtsCI GGATG 6 cut(s) 100, 409, 1026, 1054, 1078, 1168
BtsIMutI CAGTG 1 cut(s) 523
Cac8I GCNNGC 3 cut(s) 105, 201, 1125
CfoI GCGC 6 cut(s) 607, 693, 725, 757, 796, 924
Cfr13I GGNCC 4 cut(s) 295, 311, 312, 656
CseI GACGC 3 cut(s) 347, 523, 641
Csp6I GTAC 3 cut(s) 772, 841, 886
CviAII CATG 6 cut(s) 200, 472, 781, 872, 898, 926
CviQI GTAC 3 cut(s) 772, 841, 886
DdeI CTNAG 2 cut(s) 552, 867
DpnI GATC 7 cut(s) 446, 494, 569, 743, 824, 1077, 1153
DpnII GATC 7 cut(s) 444, 492, 567, 741, 822, 1075, 1151
DraI TTTAAA 1 cut(s) 355
DriI GACNNNNNGTC 1 cut(s) 862
EaeI YGGCCR 1 cut(s) 505
Eam1104I CTCTTC 4 cut(s) 93, 156, 162, 782
Eam1105I GACNNNNNGTC 1 cut(s) 862
EarI CTCTTC 4 cut(s) 93, 156, 162, 782
EciI GGCGGA 1 cut(s) 563
Eco130I CCWWGG 2 cut(s) 1053, 1095
Eco24I GRGCYC 2 cut(s) 315, 350
Eco32I GATATC 1 cut(s) 68
Eco47I GGWCC 1 cut(s) 656
Eco57I CTGAAG 1 cut(s) 1088
Eco88I CYCGRG 1 cut(s) 359
EcoO109I RGGNCCY 1 cut(s) 312
EcoRII CCWGG 2 cut(s) 843, 957
EcoRV GATATC 1 cut(s) 68
EcoT14I CCWWGG 2 cut(s) 1053, 1095
EcoT38I GRGCYC 2 cut(s) 315, 350
ErhI CCWWGG 2 cut(s) 1053, 1095
Esp3I CGTCTC 2 cut(s) 81, 870
FaeI CATG 6 cut(s) 203, 475, 784, 875, 901, 929
FaiI YATR 8 cut(s) 62, 201, 304, 473, 782, 873, 899, 927
FaqI GGGAC 1 cut(s) 542
FatI CATG 6 cut(s) 199, 471, 780, 871, 897, 925
FauI CCCGC 1 cut(s) 469
FbaI TGATCA 1 cut(s) 1075
FblI GTMKAC 1 cut(s) 15
Fnu4HI GCNGC 5 cut(s) 236, 256, 487, 603, 733
FokI GGATG 6 cut(s) 107, 396, 1033, 1061, 1085, 1155
FriOI GRGCYC 2 cut(s) 315, 350
Fsp4HI GCNGC 5 cut(s) 236, 256, 487, 603, 733
FspBI CTAG 1 cut(s) 134
GlaI GCGC 6 cut(s) 606, 692, 724, 756, 795, 923
GluI GCNGC 5 cut(s) 236, 256, 487, 603, 733
GsaI CCCAGC 2 cut(s) 312, 598
HaeIII GGCC 3 cut(s) 297, 313, 507
HapII CCGG 6 cut(s) 204, 229, 293, 422, 437, 659
HgaI GACGC 3 cut(s) 347, 523, 641
HhaI GCGC 6 cut(s) 607, 693, 725, 757, 796, 924
Hin1I GRCGYC 2 cut(s) 534, 633
Hin1II CATG 6 cut(s) 203, 475, 784, 875, 901, 929
Hin6I GCGC 6 cut(s) 605, 691, 723, 755, 794, 922
HinP1I GCGC 6 cut(s) 605, 691, 723, 755, 794, 922
HincII GTYRAC 2 cut(s) 16, 330
HindII GTYRAC 2 cut(s) 16, 330
HinfI GANTC 3 cut(s) 188, 219, 431
HpaII CCGG 6 cut(s) 204, 229, 293, 422, 437, 659
HphI GGTGA 5 cut(s) 578, 694, 796, 989, 1076
Hpy166II GTNNAC 3 cut(s) 16, 139, 330
Hpy188I TCNGA 4 cut(s) 10, 414, 547, 1119
Hpy188III TCNNGA 1 cut(s) 194
Hpy8I GTNNAC 3 cut(s) 16, 139, 330
Hpy99I CGWCG 8 cut(s) 255, 287, 341, 400, 539, 547, 635, 865
HpyAV CCTTC 7 cut(s) 60, 333, 573, 634, 951, 1018, 1063
HpyCH4IV ACGT 2 cut(s) 395, 860
HpyF10VI GCNNNNNNNGC 5 cut(s) 255, 294, 319, 729, 763
HpyF3I CTNAG 2 cut(s) 552, 867
HpySE526I ACGT 2 cut(s) 395, 860
Hsp92I GRCGYC 2 cut(s) 534, 633
Hsp92II CATG 6 cut(s) 203, 475, 784, 875, 901, 929
HspAI GCGC 6 cut(s) 605, 691, 723, 755, 794, 922
KpnI GGTACC 1 cut(s) 844
Ksp22I TGATCA 1 cut(s) 1075
Kzo9I GATC 7 cut(s) 444, 492, 567, 741, 822, 1075, 1151
LguI GCTCTTC 1 cut(s) 93
LmnI GCTCC 3 cut(s) 439, 763, 1063
LweI GCATC 5 cut(s) 775, 871, 1039, 1110, 1120
MaeI CTAG 1 cut(s) 134
MaeII ACGT 2 cut(s) 395, 860
MaeIII GTNAC 1 cut(s) 497
MalI GATC 7 cut(s) 446, 494, 569, 743, 824, 1077, 1153
MboI GATC 7 cut(s) 444, 492, 567, 741, 822, 1075, 1151
MfeI CAATTG 1 cut(s) 1013
MflI RGATCY 1 cut(s) 444
MhlI GDGCHC 2 cut(s) 315, 350
MluCI AATT 5 cut(s) 21, 951, 999, 1013, 1159
MlyI GAGTC 1 cut(s) 213
MmeI TCCRAC 2 cut(s) 100, 525
MseI TTAA 2 cut(s) 113, 354
MspA1I CMGCKG 1 cut(s) 594
MspI CCGG 6 cut(s) 204, 229, 293, 422, 437, 659
MspR9I CCNGG 5 cut(s) 294, 422, 660, 845, 959
MunI CAATTG 1 cut(s) 1013
MvaI CCWGG 2 cut(s) 845, 959
MvnI CGCG 7 cut(s) 255, 341, 607, 680, 693, 757, 796
MwoI GCNNNNNNNGC 5 cut(s) 255, 294, 319, 729, 763
NciI CCSGG 3 cut(s) 294, 422, 660
NdeII GATC 7 cut(s) 444, 492, 567, 741, 822, 1075, 1151
NlaIII CATG 6 cut(s) 203, 475, 784, 875, 901, 929
NlaIV GGNNCC 5 cut(s) 313, 314, 657, 717, 842
NmuCI GTSAC 1 cut(s) 497
NspI RCATGY 1 cut(s) 203
PaeI GCATGC 1 cut(s) 203
PciSI GCTCTTC 1 cut(s) 93
PcsI WCGNNNNNNNCGW 2 cut(s) 274, 627
PfeI GAWTC 2 cut(s) 188, 431
PflFI GACNNNGTC 1 cut(s) 533
PfoI TCCNGGA 1 cut(s) 658
PkrI GCNGC 5 cut(s) 237, 257, 488, 604, 734
PleI GAGTC 1 cut(s) 213
PpsI GAGTC 1 cut(s) 213
Psp6I CCWGG 2 cut(s) 843, 957
PspFI CCCAGC 2 cut(s) 308, 594
PspGI CCWGG 2 cut(s) 843, 957
PspN4I GGNNCC 5 cut(s) 313, 314, 657, 717, 842
PspOMI GGGCCC 1 cut(s) 311
PspPI GGNCC 4 cut(s) 295, 311, 312, 656
PsuI RGATCY 1 cut(s) 444
PsyI GACNNNGTC 1 cut(s) 533
RsaI GTAC 3 cut(s) 773, 842, 887
RsaNI GTAC 3 cut(s) 772, 841, 886
SalI GTCGAC 1 cut(s) 14
SapI GCTCTTC 1 cut(s) 93
SaqAI TTAA 2 cut(s) 113, 354
SatI GCNGC 5 cut(s) 236, 256, 487, 603, 733
Sau3AI GATC 7 cut(s) 444, 492, 567, 741, 822, 1075, 1151
Sau96I GGNCC 4 cut(s) 295, 311, 312, 656
SchI GAGTC 1 cut(s) 213
ScrFI CCNGG 5 cut(s) 294, 422, 660, 845, 959
SduI GDGCHC 2 cut(s) 315, 350
SfaNI GCATC 5 cut(s) 775, 871, 1039, 1110, 1120
SinI GGWCC 1 cut(s) 656
SmlI CTYRAG 2 cut(s) 192, 1139
SmoI CTYRAG 2 cut(s) 192, 1139
SphI GCATGC 1 cut(s) 203
Sse9I AATT 5 cut(s) 21, 951, 999, 1013, 1159
SspMI CTAG 1 cut(s) 134
StyD4I CCNGG 5 cut(s) 292, 420, 658, 843, 957
StyI CCWWGG 2 cut(s) 1053, 1095
TaiI ACGT 2 cut(s) 398, 863
TaqI TCGA 3 cut(s) 15, 566, 630
TasI AATT 5 cut(s) 21, 951, 999, 1013, 1159
TauI GCSGC 5 cut(s) 238, 258, 489, 605, 735
TfiI GAWTC 2 cut(s) 188, 431
Tru1I TTAA 2 cut(s) 113, 354
Tru9I TTAA 2 cut(s) 113, 354
TscAI CASTG 1 cut(s) 530
TseFI GTSAC 1 cut(s) 497
Tsp45I GTSAC 1 cut(s) 497
TspDTI ATGAA 4 cut(s) 17, 111, 439, 1037
TspGWI ACGGA 2 cut(s) 485, 531
TspRI CASTG 1 cut(s) 530
Tth111I GACNNNGTC 1 cut(s) 533
VpaK11BI GGWCC 1 cut(s) 656
XceI RCATGY 1 cut(s) 203
XcmI CCANNNNNNNNNTGG 1 cut(s) 305
XmiI GTMKAC 1 cut(s) 15
XspI CTAG 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.