RLG00000014839

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003321
Physical Location & Seq
Reverse (-)
58227740 .. 58229413
1674 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014839

Sequence Viewer

Length: 1194 bp
ATGAACTCCGAAGTCGACCCAATTCGTATTCCCTTTTCTTCTCGTTTCCCTTCAAACCCTTTGCGATATCGTTTCCCTTCAAACCTTTTGCGATATCGTCTCCAACAAATGAGAGACGATGATGATGAAGAACAGGCTCTTTTAACCCATTTGCTTACTCTCTTATCTCAGTATCTCAAGGACAAAGAGGAGGAGGCGGATCCTCAAGAACGTGCCGGAGAACAACACCGACTCGCTAACCTGAAGCGGCGAAGAACTCGCCGTCGCGGCACAACTAAGCGAAGTAGACGAAACGACGGCGTTCCGCGCCAAGCCTACGCTGGGCCCCACTCGCTTTCGTTGACAAGCGACGCGGAGAGCCCATTTAAACCCGAGAACTCGCTCATTCCAAGAACAAAACCCGACACGTCGTTTATCCTCCATCCGAAAACCCCGGAAATGGAATCGCCGGAGCAGATCTTCATTCTTTCGGGGCAGAGCAACATGGCGGGTCGGGGCGGCGTGATCCGTGACCACGGCCACAACCACCACCAGCACTGGGACGGCGTCGTTCCGTCGGAGGCTCAGCCCGACCCTTCGATCCTCCGCCTCAACGCTCACCTCCACTGGGAGGCGGCGCGTGAGCCTCTCCACGCCGATATCGACGCCAAGAAGGTGTGCGGTTTGGGTCCGGGAATGTCGTTTGCCAACGCGGTCAGGGGGCGCGTGGAGGGGAAGATTGGGCTGGTGCCGTGCGCGGTTGGCGGTACGGCGATCAAGGAGTGGGCGCGTGGGGAGCATCTGTACGAGAACATGGTGAAGAGGGCGCGTGAGAGCGTGAAGAACGGGGGAGAGATCAAAGGGTTGCTGTGGTACCAGGGGGAGAGCGACACGTCGTCTCAGCACGATGTTGATGCGTACCACGAGAACATGGTCAGGCTTATTGAGAACGTGCGCCATGACCTTGCTTTGCCTTCACTTCCAATAATCCAGGTGGCTATTTGTTCTGGTGATGAGAAGTTCTTGGAGAAAATTAGGGAGGTTCAATTGGGGTTGAAGGTTGAGAATGTTGCGTGTGTGGACGCCAAGGGATTGGAGCTGAAAGATGATCATCTTCACCTGACTACCAAGGCTCAAGTTCAGTTGGGTCAGATGCTTGCTGATGCTTACCTCAAGCATTTTGGATCATCTAAATCCCATCATCCTGCCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

398

Amino Acids

44.71

Weight (kDa)

7.37

Isoelectric Point (pI)

42.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 151 - 385 1.4e-95 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 852
AccB1I GGYRCC 2 cut(s) 727, 852
AccI GTMKAC 2 cut(s) 15, 286
AccII CGCG 9 cut(s) 267, 307, 353, 619, 692, 705, 737, 769, 808
AclWI GGATC 5 cut(s) 194, 207, 499, 574, 1171
AcoI YGGCCR 1 cut(s) 517
AcuI CTGAAG 1 cut(s) 263
AcyI GRCGYC 3 cut(s) 546, 645, 1062
AfaI GTAC 4 cut(s) 748, 785, 854, 899
AfiI CCNNNNNNNGG 5 cut(s) 321, 439, 538, 607, 610
AflIII ACRYGT 2 cut(s) 405, 870
AgsI TTSAA 4 cut(s) 54, 81, 1025, 1036
AhdI GACNNNNNGTC 1 cut(s) 874
AjiI CACGTC 2 cut(s) 408, 873
AjnI CCWGG 2 cut(s) 855, 969
AluBI AGCT 1 cut(s) 1078
AluI AGCT 1 cut(s) 1078
Alw26I GTCTC 3 cut(s) 104, 108, 882
AlwI GGATC 5 cut(s) 194, 207, 499, 574, 1171
Ama87I CYCGRG 1 cut(s) 371
AoxI GGCC 2 cut(s) 323, 517
ApaI GGGCCC 1 cut(s) 327
Asp718I GGTACC 1 cut(s) 852
AspLEI GCGC 7 cut(s) 309, 619, 705, 737, 769, 808, 936
AspS9I GGNCC 3 cut(s) 323, 324, 668
AsuC2I CCSGG 2 cut(s) 434, 672
AsuHPI GGTGA 4 cut(s) 590, 808, 1001, 1088
AvaI CYCGRG 1 cut(s) 371
AvaII GGWCC 1 cut(s) 668
BaeGI GKGCMC 1 cut(s) 327
BamHI GGATCC 1 cut(s) 199
BanI GGYRCC 2 cut(s) 727, 852
BanII GRGCYC 2 cut(s) 327, 362
BauI CACGAG 1 cut(s) 902
BccI CCATC 2 cut(s) 429, 1185
BceAI ACGGC 6 cut(s) 246, 313, 532, 559, 715, 765
BcgI CGANNNNNNTGC 2 cut(s) 875, 909
BciT130I CCWGG 2 cut(s) 857, 971
BclI TGATCA 1 cut(s) 1087
BcnI CCSGG 2 cut(s) 434, 672
BcoDI GTCTC 3 cut(s) 104, 108, 882
BglI GCCNNNNNGGC 1 cut(s) 267
BglII AGATCT 1 cut(s) 456
BisI GCNGC 4 cut(s) 248, 268, 499, 615
BlpI GCTNAGC 1 cut(s) 564
BlsI GCNGC 4 cut(s) 249, 269, 500, 616
Bme1390I CCNGG 4 cut(s) 434, 672, 857, 971
Bme18I GGWCC 1 cut(s) 668
BmeRI GACNNNNNGTC 1 cut(s) 874
BmeT110I CYCGRG 1 cut(s) 371
BmgBI CACGTC 2 cut(s) 408, 873
BmgT120I GGNCC 3 cut(s) 323, 324, 668
BmiI GGNNCC 6 cut(s) 201, 325, 326, 669, 729, 854
BmrFI CCNGG 4 cut(s) 434, 672, 857, 971
BmrI ACTGGG 2 cut(s) 547, 616
BmsI GCATC 4 cut(s) 787, 883, 1122, 1132
BmuI ACTGGG 2 cut(s) 547, 616
Bpu1102I GCTNAGC 1 cut(s) 564
BpuEI CTTGAG 4 cut(s) 161, 189, 1098, 1136
BpuMI CCSGG 2 cut(s) 434, 672
BsaBI GATNNNNATC 1 cut(s) 1089
BsaHI GRCGYC 3 cut(s) 546, 645, 1062
BsaJI CCNNGG 5 cut(s) 432, 514, 856, 1065, 1107
BsaXI ACNNNNNCTCC 2 cut(s) 767, 797
Bsc4I CCNNNNNNNGG 5 cut(s) 321, 439, 538, 607, 610
Bse1I ACTGG 2 cut(s) 542, 611
Bse8I GATNNNNATC 1 cut(s) 1089
BseBI CCWGG 2 cut(s) 857, 971
BseDI CCNNGG 5 cut(s) 432, 514, 856, 1065, 1107
BseGI GGATG 2 cut(s) 421, 1180
BseJI GATNNNNATC 1 cut(s) 1089
BseLI CCNNNNNNNGG 5 cut(s) 321, 439, 538, 607, 610
BseMII CTCAG 3 cut(s) 182, 578, 893
BseNI ACTGG 2 cut(s) 542, 611
BseRI GAGGAG 2 cut(s) 203, 206
BseSI GKGCMC 1 cut(s) 327
BseYI CCCAGC 1 cut(s) 320
Bsh1236I CGCG 9 cut(s) 267, 307, 353, 619, 692, 705, 737, 769, 808
BshFI GGCC 2 cut(s) 325, 519
BshNI GGYRCC 2 cut(s) 727, 852
BsiHKCI CYCGRG 1 cut(s) 371
BsiSI CCGG 4 cut(s) 216, 434, 449, 671
BslFI GGGAC 1 cut(s) 554
BslI CCNNNNNNNGG 5 cut(s) 321, 439, 538, 607, 610
BsmAI GTCTC 3 cut(s) 104, 108, 882
BsmBI CGTCTC 3 cut(s) 104, 108, 882
BsmFI GGGAC 1 cut(s) 554
BsnI GGCC 2 cut(s) 325, 519
BsoBI CYCGRG 1 cut(s) 371
Bsp120I GGGCCC 1 cut(s) 323
Bsp1286I GDGCHC 2 cut(s) 327, 362
Bsp143I GATC 8 cut(s) 199, 456, 504, 579, 753, 834, 1087, 1163
Bsp1720I GCTNAGC 1 cut(s) 564
BspANI GGCC 2 cut(s) 325, 519
BspCNI CTCAG 3 cut(s) 181, 577, 892
BspFNI CGCG 9 cut(s) 267, 307, 353, 619, 692, 705, 737, 769, 808
BspLI GGNNCC 6 cut(s) 201, 325, 326, 669, 729, 854
BspPI GGATC 5 cut(s) 194, 207, 499, 574, 1171
BspT107I GGYRCC 2 cut(s) 727, 852
BsrI ACTGG 2 cut(s) 542, 611
BssECI CCNNGG 5 cut(s) 432, 514, 856, 1065, 1107
BssMI GATC 8 cut(s) 199, 456, 504, 579, 753, 834, 1087, 1163
BssNI GRCGYC 3 cut(s) 546, 645, 1062
BssSI CACGAG 1 cut(s) 902
BssT1I CCWWGG 2 cut(s) 1065, 1107
Bst2BI CACGAG 1 cut(s) 902
Bst2UI CCWGG 2 cut(s) 857, 971
Bst6I CTCTTC 1 cut(s) 794
BstACI GRCGYC 3 cut(s) 546, 645, 1062
BstC8I GCNNGC 1 cut(s) 1137
BstDEI CTNAG 4 cut(s) 168, 276, 564, 879
BstDSI CCRYGG 1 cut(s) 514
BstF5I GGATG 2 cut(s) 421, 1180
BstFNI CGCG 9 cut(s) 267, 307, 353, 619, 692, 705, 737, 769, 808
BstHHI GCGC 7 cut(s) 309, 619, 705, 737, 769, 808, 936
BstKTI GATC 8 cut(s) 202, 459, 507, 582, 756, 837, 1090, 1166
BstMAI GTCTC 3 cut(s) 104, 108, 882
BstMBI GATC 8 cut(s) 199, 456, 504, 579, 753, 834, 1087, 1163
BstMWI GCNNNNNNNGC 5 cut(s) 267, 306, 331, 741, 775
BstNI CCWGG 2 cut(s) 857, 971
BstSCI CCNGG 4 cut(s) 432, 670, 855, 969
BstSLI GKGCMC 1 cut(s) 327
BstUI CGCG 9 cut(s) 267, 307, 353, 619, 692, 705, 737, 769, 808
BstX2I RGATCY 2 cut(s) 199, 456
BstXI CCANNNNNNTGG 1 cut(s) 1072
BstYI RGATCY 2 cut(s) 199, 456
BsuRI GGCC 2 cut(s) 325, 519
BtgI CCRYGG 1 cut(s) 514
BtrI CACGTC 2 cut(s) 408, 873
BtsCI GGATG 2 cut(s) 421, 1180
BtsIMutI CAGTG 2 cut(s) 535, 604
Cac8I GCNNGC 1 cut(s) 1137
CfoI GCGC 7 cut(s) 309, 619, 705, 737, 769, 808, 936
Cfr13I GGNCC 3 cut(s) 323, 324, 668
CseI GACGC 4 cut(s) 359, 535, 653, 1070
Csp6I GTAC 4 cut(s) 747, 784, 853, 898
CviAII CATG 4 cut(s) 484, 793, 910, 938
CviQI GTAC 4 cut(s) 747, 784, 853, 898
DdeI CTNAG 4 cut(s) 168, 276, 564, 879
DpnI GATC 8 cut(s) 201, 458, 506, 581, 755, 836, 1089, 1165
DpnII GATC 8 cut(s) 199, 456, 504, 579, 753, 834, 1087, 1163
DraI TTTAAA 1 cut(s) 367
DriI GACNNNNNGTC 1 cut(s) 874
EaeI YGGCCR 1 cut(s) 517
Eam1104I CTCTTC 1 cut(s) 794
Eam1105I GACNNNNNGTC 1 cut(s) 874
EarI CTCTTC 1 cut(s) 794
EciI GGCGGA 2 cut(s) 212, 575
Eco130I CCWWGG 2 cut(s) 1065, 1107
Eco24I GRGCYC 2 cut(s) 327, 362
Eco32I GATATC 3 cut(s) 68, 95, 640
Eco47I GGWCC 1 cut(s) 668
Eco57I CTGAAG 1 cut(s) 263
Eco88I CYCGRG 1 cut(s) 371
EcoO109I RGGNCCY 1 cut(s) 324
EcoRII CCWGG 2 cut(s) 855, 969
EcoRV GATATC 3 cut(s) 68, 95, 640
EcoT14I CCWWGG 2 cut(s) 1065, 1107
EcoT38I GRGCYC 2 cut(s) 327, 362
ErhI CCWWGG 2 cut(s) 1065, 1107
Esp3I CGTCTC 3 cut(s) 104, 108, 882
FaeI CATG 4 cut(s) 487, 796, 913, 941
FaiI YATR 4 cut(s) 485, 794, 911, 939
FaqI GGGAC 1 cut(s) 554
FatI CATG 4 cut(s) 483, 792, 909, 937
FauI CCCGC 1 cut(s) 481
FbaI TGATCA 1 cut(s) 1087
FblI GTMKAC 2 cut(s) 15, 286
Fnu4HI GCNGC 4 cut(s) 248, 268, 499, 615
FokI GGATG 2 cut(s) 408, 1167
FriOI GRGCYC 2 cut(s) 327, 362
Fsp4HI GCNGC 4 cut(s) 248, 268, 499, 615
GlaI GCGC 7 cut(s) 308, 618, 704, 736, 768, 807, 935
GluI GCNGC 4 cut(s) 248, 268, 499, 615
GsaI CCCAGC 1 cut(s) 324
HaeIII GGCC 2 cut(s) 325, 519
HapII CCGG 4 cut(s) 216, 434, 449, 671
HgaI GACGC 4 cut(s) 359, 535, 653, 1070
HhaI GCGC 7 cut(s) 309, 619, 705, 737, 769, 808, 936
Hin1I GRCGYC 3 cut(s) 546, 645, 1062
Hin1II CATG 4 cut(s) 487, 796, 913, 941
Hin6I GCGC 7 cut(s) 307, 617, 703, 735, 767, 806, 934
HinP1I GCGC 7 cut(s) 307, 617, 703, 735, 767, 806, 934
HincII GTYRAC 2 cut(s) 16, 342
HindII GTYRAC 2 cut(s) 16, 342
HinfI GANTC 2 cut(s) 231, 443
HpaII CCGG 4 cut(s) 216, 434, 449, 671
HphI GGTGA 4 cut(s) 590, 808, 1001, 1088
Hpy166II GTNNAC 4 cut(s) 16, 287, 342, 1060
Hpy188I TCNGA 4 cut(s) 10, 426, 559, 1131
Hpy188III TCNNGA 1 cut(s) 206
Hpy8I GTNNAC 4 cut(s) 16, 287, 342, 1060
Hpy99I CGWCG 8 cut(s) 267, 299, 353, 412, 551, 559, 647, 877
HpyAV CCTTC 6 cut(s) 60, 87, 585, 646, 963, 1030
HpyCH4IV ACGT 4 cut(s) 211, 407, 872, 930
HpyF10VI GCNNNNNNNGC 5 cut(s) 267, 306, 331, 741, 775
HpyF3I CTNAG 4 cut(s) 168, 276, 564, 879
HpySE526I ACGT 4 cut(s) 211, 407, 872, 930
Hsp92I GRCGYC 3 cut(s) 546, 645, 1062
Hsp92II CATG 4 cut(s) 487, 796, 913, 941
HspAI GCGC 7 cut(s) 307, 617, 703, 735, 767, 806, 934
KpnI GGTACC 1 cut(s) 856
Ksp22I TGATCA 1 cut(s) 1087
Kzo9I GATC 8 cut(s) 199, 456, 504, 579, 753, 834, 1087, 1163
LmnI GCTCC 3 cut(s) 451, 775, 1075
LweI GCATC 4 cut(s) 787, 883, 1122, 1132
MaeII ACGT 4 cut(s) 211, 407, 872, 930
MaeIII GTNAC 1 cut(s) 509
MalI GATC 8 cut(s) 201, 458, 506, 581, 755, 836, 1089, 1165
MboI GATC 8 cut(s) 199, 456, 504, 579, 753, 834, 1087, 1163
MboII GAAGA 8 cut(s) 30, 140, 264, 451, 727, 811, 832, 1085
MfeI CAATTG 1 cut(s) 1025
MflI RGATCY 2 cut(s) 199, 456
MhlI GDGCHC 2 cut(s) 327, 362
MluCI AATT 3 cut(s) 21, 1011, 1025
MlyI GAGTC 1 cut(s) 225
MmeI TCCRAC 2 cut(s) 127, 537
MseI TTAA 2 cut(s) 143, 366
MspI CCGG 4 cut(s) 216, 434, 449, 671
MspR9I CCNGG 4 cut(s) 434, 672, 857, 971
MunI CAATTG 1 cut(s) 1025
MvaI CCWGG 2 cut(s) 857, 971
MvnI CGCG 9 cut(s) 267, 307, 353, 619, 692, 705, 737, 769, 808
MwoI GCNNNNNNNGC 5 cut(s) 267, 306, 331, 741, 775
NciI CCSGG 2 cut(s) 434, 672
NdeII GATC 8 cut(s) 199, 456, 504, 579, 753, 834, 1087, 1163
NlaIII CATG 4 cut(s) 487, 796, 913, 941
NlaIV GGNNCC 6 cut(s) 201, 325, 326, 669, 729, 854
NmuCI GTSAC 1 cut(s) 509
PcsI WCGNNNNNNNCGW 1 cut(s) 639
PfeI GAWTC 1 cut(s) 443
PflFI GACNNNGTC 1 cut(s) 545
PfoI TCCNGGA 1 cut(s) 670
PkrI GCNGC 4 cut(s) 249, 269, 500, 616
PleI GAGTC 1 cut(s) 225
PpsI GAGTC 1 cut(s) 225
Psp6I CCWGG 2 cut(s) 855, 969
PspFI CCCAGC 1 cut(s) 320
PspGI CCWGG 2 cut(s) 855, 969
PspN4I GGNNCC 6 cut(s) 201, 325, 326, 669, 729, 854
PspOMI GGGCCC 1 cut(s) 323
PspPI GGNCC 3 cut(s) 323, 324, 668
PsuI RGATCY 2 cut(s) 199, 456
PsyI GACNNNGTC 1 cut(s) 545
RsaI GTAC 4 cut(s) 748, 785, 854, 899
RsaNI GTAC 4 cut(s) 747, 784, 853, 898
SalI GTCGAC 1 cut(s) 14
SaqAI TTAA 2 cut(s) 143, 366
SatI GCNGC 4 cut(s) 248, 268, 499, 615
Sau3AI GATC 8 cut(s) 199, 456, 504, 579, 753, 834, 1087, 1163
Sau96I GGNCC 3 cut(s) 323, 324, 668
SchI GAGTC 1 cut(s) 225
ScrFI CCNGG 4 cut(s) 434, 672, 857, 971
SduI GDGCHC 2 cut(s) 327, 362
SfaNI GCATC 4 cut(s) 787, 883, 1122, 1132
SinI GGWCC 1 cut(s) 668
SmlI CTYRAG 4 cut(s) 176, 204, 1113, 1151
SmoI CTYRAG 4 cut(s) 176, 204, 1113, 1151
Sse9I AATT 3 cut(s) 21, 1011, 1025
StyD4I CCNGG 4 cut(s) 432, 670, 855, 969
StyI CCWWGG 2 cut(s) 1065, 1107
TaiI ACGT 4 cut(s) 214, 410, 875, 933
TaqI TCGA 3 cut(s) 15, 578, 642
TasI AATT 3 cut(s) 21, 1011, 1025
TauI GCSGC 4 cut(s) 250, 270, 501, 617
TfiI GAWTC 1 cut(s) 443
Tru1I TTAA 2 cut(s) 143, 366
Tru9I TTAA 2 cut(s) 143, 366
TscAI CASTG 2 cut(s) 542, 611
TseFI GTSAC 1 cut(s) 509
Tsp45I GTSAC 1 cut(s) 509
TspDTI ATGAA 3 cut(s) 17, 141, 451
TspGWI ACGGA 2 cut(s) 497, 543
TspRI CASTG 2 cut(s) 542, 611
Tth111I GACNNNGTC 1 cut(s) 545
VpaK11BI GGWCC 1 cut(s) 668
XcmI CCANNNNNNNNNTGG 1 cut(s) 317
XmiI GTMKAC 2 cut(s) 15, 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.