Rh6BG068600

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0003321
Physical Location & Seq
Forward (+)
10540821 .. 10542748
1928 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG068600.1

Sequence Viewer

Length: 1188 bp
ATGAACTCCGAAGTCGACCCAATTCGTATTCCATTTTCTTCTCGTTTCCCTTCAAACCTTAAGCGATATCTTCTCCAACGAATGAGAGACGAGGATGAAGAGCAGGCTCTTTTAACCCATTTGCTTACTCTCCTAGTTCACTATCTCAACGACAAAGAGGAAGAGGAGGAGGAGGAGGAGGAGGATTCTCAAGAGCATGCCGGAGAACAACACCGACTCGCTAACCGGAAGCGGCGAAGAACTCGCCGTCGCGGCACAACTACACGAAGAAGACGAAACGACGGCGTTCCGCGCCAAGCCTACGCTGGGCCCCACTCGCCTGCTTTGACAAGCGACGCGGAGAGCCCATTTAAACCAGAGAACTCGCTCATTCCAAGAACAAAACCCGACACGTCGTTTATCCTCCACCCGAAAAACCCGGAAATGGAGTCGCCGGAGCAGATCTTCATTCTTTCGGGGCAGAGCAACATGGCGGGTCGGGGCGGCGTGATCCGTGACCACGGCCACAACCACCACCAGCACTGGGACGGCGTCGTTCCGTCGGAGGCTCAGCCCGACCCTTCGATCCTCCGCCTCAACGCTCACCTCCACTGGGAGGCGGCGCGTGAGCCTCTCCACGCCGACATCGACTCCAAGAAGGTGTGCGGTTTGGGTCCGGGAATGTCGTTCGCCAACGCGGTGAGGGGGCGCGTGGAGGGGAAGATTGGGCTGGTGCCGTGCGCGGTTGGCGGCACGGCGATCAAGGAGTGGGCGCGTGGGGAGCATCTGTACGAGAACATGGTGAAGAGGGCGCGTGAGAGCGTGAAGAACGGGGGAGAGATCAAAGGGTTGCTGTGGTACCAGGGGGAGAGCGACACGTCGTCTCAGCATGATGTTGATGCGTACCACGAGAACATGGTTAGGCTCATTGAGAATGTGCGCCATGACCTTGCTTTGCCTTCACTTCCAATTATCCAGGTGGCTATTTGTTCTGGTGATGAGAAGTTCTTGGAGAAAATTAGGGAGGTTCAATTGGGGATGAAGGTTGAGAATGTTGTGTGTGTGGATGCCAAGGGATTGGAGCTGAAGGATGATCATCTTCACCTGACTACCAAGGCACAAGTTCAGTTGGGTCAGATGCTGGCTGATGCTTACCTCAAGCATTTTGGACCAGCTAATTCCGATCATCCTGCGATTCCTGCCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

395

Amino Acids

44.52

Weight (kDa)

6.2

Isoelectric Point (pI)

51.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 146 - 380 7.4e-96 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 837
AccB1I GGYRCC 2 cut(s) 712, 837
AccI GTMKAC 1 cut(s) 15
AccII CGCG 9 cut(s) 252, 292, 338, 604, 677, 690, 722, 754, 793
AclWI GGATC 2 cut(s) 484, 559
AcoI YGGCCR 1 cut(s) 502
AcuI CTGAAG 1 cut(s) 1085
AcyI GRCGYC 1 cut(s) 531
AfaI GTAC 3 cut(s) 770, 839, 884
AfiI CCNNNNNNNGG 5 cut(s) 306, 424, 523, 592, 595
AflII CTTAAG 1 cut(s) 59
AflIII ACRYGT 2 cut(s) 390, 855
AgsI TTSAA 2 cut(s) 54, 1010
AhdI GACNNNNNGTC 1 cut(s) 859
AjiI CACGTC 2 cut(s) 393, 858
AjnI CCWGG 2 cut(s) 840, 954
AluBI AGCT 2 cut(s) 1063, 1154
AluI AGCT 2 cut(s) 1063, 1154
Alw26I GTCTC 2 cut(s) 81, 867
AlwI GGATC 2 cut(s) 484, 559
AlwNI CAGNNNCTG 1 cut(s) 1120
AoxI GGCC 2 cut(s) 308, 502
ApaI GGGCCC 1 cut(s) 312
Asp718I GGTACC 1 cut(s) 837
AspLEI GCGC 7 cut(s) 294, 604, 690, 722, 754, 793, 921
AspS9I GGNCC 4 cut(s) 308, 309, 653, 1148
AsuC2I CCSGG 2 cut(s) 419, 657
AsuHPI GGTGA 5 cut(s) 575, 691, 793, 986, 1073
AvaII GGWCC 2 cut(s) 653, 1148
BaeGI GKGCMC 1 cut(s) 312
BanI GGYRCC 2 cut(s) 712, 837
BanII GRGCYC 2 cut(s) 312, 347
BauI CACGAG 1 cut(s) 887
BbsI GAAGAC 1 cut(s) 277
BceAI ACGGC 6 cut(s) 231, 298, 517, 544, 700, 750
BcgI CGANNNNNNTGC 2 cut(s) 1151, 1185
BciT130I CCWGG 2 cut(s) 842, 956
BclI TGATCA 1 cut(s) 1072
BcnI CCSGG 2 cut(s) 419, 657
BcoDI GTCTC 2 cut(s) 81, 867
BfaI CTAG 1 cut(s) 134
BfrI CTTAAG 1 cut(s) 59
BglI GCCNNNNNGGC 1 cut(s) 252
BglII AGATCT 1 cut(s) 441
BisI GCNGC 5 cut(s) 233, 253, 484, 600, 730
BlpI GCTNAGC 1 cut(s) 549
BlsI GCNGC 5 cut(s) 234, 254, 485, 601, 731
Bme1390I CCNGG 4 cut(s) 419, 657, 842, 956
Bme18I GGWCC 2 cut(s) 653, 1148
BmeRI GACNNNNNGTC 1 cut(s) 859
BmgBI CACGTC 2 cut(s) 393, 858
BmgT120I GGNCC 4 cut(s) 308, 309, 653, 1148
BmiI GGNNCC 5 cut(s) 310, 311, 654, 714, 839
BmrFI CCNGG 4 cut(s) 419, 657, 842, 956
BmrI ACTGGG 2 cut(s) 532, 601
BmsI GCATC 5 cut(s) 772, 868, 1036, 1107, 1117
BmuI ACTGGG 2 cut(s) 532, 601
BpiI GAAGAC 1 cut(s) 277
Bpu1102I GCTNAGC 1 cut(s) 549
BpuEI CTTGAG 2 cut(s) 174, 1121
BpuMI CCSGG 2 cut(s) 419, 657
BsaBI GATNNNNATC 1 cut(s) 1074
BsaHI GRCGYC 1 cut(s) 531
BsaJI CCNNGG 4 cut(s) 499, 841, 1050, 1092
BsaWI WCCGGW 1 cut(s) 225
BsaXI ACNNNNNCTCC 4 cut(s) 614, 644, 752, 782
Bsc4I CCNNNNNNNGG 5 cut(s) 306, 424, 523, 592, 595
Bse1I ACTGG 2 cut(s) 527, 596
Bse8I GATNNNNATC 1 cut(s) 1074
BseBI CCWGG 2 cut(s) 842, 956
BseDI CCNNGG 4 cut(s) 499, 841, 1050, 1092
BseGI GGATG 5 cut(s) 100, 1023, 1051, 1075, 1165
BseJI GATNNNNATC 1 cut(s) 1074
BseLI CCNNNNNNNGG 5 cut(s) 306, 424, 523, 592, 595
BseMII CTCAG 2 cut(s) 563, 878
BseNI ACTGG 2 cut(s) 527, 596
BseRI GAGGAG 6 cut(s) 179, 182, 185, 188, 191, 194
BseSI GKGCMC 1 cut(s) 312
BseYI CCCAGC 1 cut(s) 305
Bsh1236I CGCG 9 cut(s) 252, 292, 338, 604, 677, 690, 722, 754, 793
BshFI GGCC 2 cut(s) 310, 504
BshNI GGYRCC 2 cut(s) 712, 837
BsiSI CCGG 5 cut(s) 201, 226, 419, 434, 656
BslFI GGGAC 1 cut(s) 539
BslI CCNNNNNNNGG 5 cut(s) 306, 424, 523, 592, 595
BsmAI GTCTC 2 cut(s) 81, 867
BsmBI CGTCTC 2 cut(s) 81, 867
BsmFI GGGAC 1 cut(s) 539
BsnI GGCC 2 cut(s) 310, 504
Bsp120I GGGCCC 1 cut(s) 308
Bsp1286I GDGCHC 2 cut(s) 312, 347
Bsp143I GATC 7 cut(s) 441, 489, 564, 738, 819, 1072, 1162
Bsp1720I GCTNAGC 1 cut(s) 549
BspANI GGCC 2 cut(s) 310, 504
BspCNI CTCAG 2 cut(s) 562, 877
BspFNI CGCG 9 cut(s) 252, 292, 338, 604, 677, 690, 722, 754, 793
BspLI GGNNCC 5 cut(s) 310, 311, 654, 714, 839
BspPI GGATC 2 cut(s) 484, 559
BspQI GCTCTTC 1 cut(s) 93
BspT107I GGYRCC 2 cut(s) 712, 837
BspTI CTTAAG 1 cut(s) 59
BsrI ACTGG 2 cut(s) 527, 596
BssECI CCNNGG 4 cut(s) 499, 841, 1050, 1092
BssMI GATC 7 cut(s) 441, 489, 564, 738, 819, 1072, 1162
BssNI GRCGYC 1 cut(s) 531
BssSI CACGAG 1 cut(s) 887
BssT1I CCWWGG 2 cut(s) 1050, 1092
Bst2BI CACGAG 1 cut(s) 887
Bst2UI CCWGG 2 cut(s) 842, 956
Bst6I CTCTTC 3 cut(s) 93, 156, 779
BstACI GRCGYC 1 cut(s) 531
BstAFI CTTAAG 1 cut(s) 59
BstC8I GCNNGC 4 cut(s) 105, 198, 321, 1122
BstDEI CTNAG 2 cut(s) 549, 864
BstDSI CCRYGG 1 cut(s) 499
BstF5I GGATG 5 cut(s) 100, 1023, 1051, 1075, 1165
BstFNI CGCG 9 cut(s) 252, 292, 338, 604, 677, 690, 722, 754, 793
BstHHI GCGC 7 cut(s) 294, 604, 690, 722, 754, 793, 921
BstKTI GATC 7 cut(s) 444, 492, 567, 741, 822, 1075, 1165
BstMAI GTCTC 2 cut(s) 81, 867
BstMBI GATC 7 cut(s) 441, 489, 564, 738, 819, 1072, 1162
BstMWI GCNNNNNNNGC 6 cut(s) 252, 291, 316, 726, 760, 1178
BstNI CCWGG 2 cut(s) 842, 956
BstNSI RCATGY 1 cut(s) 200
BstSCI CCNGG 4 cut(s) 417, 655, 840, 954
BstSLI GKGCMC 1 cut(s) 312
BstUI CGCG 9 cut(s) 252, 292, 338, 604, 677, 690, 722, 754, 793
BstV2I GAAGAC 1 cut(s) 277
BstX2I RGATCY 1 cut(s) 441
BstXI CCANNNNNNTGG 1 cut(s) 1057
BstYI RGATCY 1 cut(s) 441
BsuRI GGCC 2 cut(s) 310, 504
BtgI CCRYGG 1 cut(s) 499
BtrI CACGTC 2 cut(s) 393, 858
BtsCI GGATG 5 cut(s) 100, 1023, 1051, 1075, 1165
BtsIMutI CAGTG 2 cut(s) 520, 589
Cac8I GCNNGC 4 cut(s) 105, 198, 321, 1122
CaiI CAGNNNCTG 1 cut(s) 1120
CfoI GCGC 7 cut(s) 294, 604, 690, 722, 754, 793, 921
Cfr13I GGNCC 4 cut(s) 308, 309, 653, 1148
CseI GACGC 2 cut(s) 344, 520
Csp6I GTAC 3 cut(s) 769, 838, 883
CviAII CATG 6 cut(s) 197, 469, 778, 869, 895, 923
CviQI GTAC 3 cut(s) 769, 838, 883
DdeI CTNAG 2 cut(s) 549, 864
DpnI GATC 7 cut(s) 443, 491, 566, 740, 821, 1074, 1164
DpnII GATC 7 cut(s) 441, 489, 564, 738, 819, 1072, 1162
DraI TTTAAA 1 cut(s) 352
DriI GACNNNNNGTC 1 cut(s) 859
EaeI YGGCCR 1 cut(s) 502
Eam1104I CTCTTC 3 cut(s) 93, 156, 779
Eam1105I GACNNNNNGTC 1 cut(s) 859
EarI CTCTTC 3 cut(s) 93, 156, 779
EciI GGCGGA 1 cut(s) 560
Eco130I CCWWGG 2 cut(s) 1050, 1092
Eco24I GRGCYC 2 cut(s) 312, 347
Eco32I GATATC 1 cut(s) 68
Eco47I GGWCC 2 cut(s) 653, 1148
Eco57I CTGAAG 1 cut(s) 1085
EcoO109I RGGNCCY 1 cut(s) 309
EcoRII CCWGG 2 cut(s) 840, 954
EcoRV GATATC 1 cut(s) 68
EcoT14I CCWWGG 2 cut(s) 1050, 1092
EcoT38I GRGCYC 2 cut(s) 312, 347
ErhI CCWWGG 2 cut(s) 1050, 1092
Esp3I CGTCTC 2 cut(s) 81, 867
FaeI CATG 6 cut(s) 200, 472, 781, 872, 898, 926
FaiI YATR 6 cut(s) 198, 470, 779, 870, 896, 924
FaqI GGGAC 1 cut(s) 539
FatI CATG 6 cut(s) 196, 468, 777, 868, 894, 922
FauI CCCGC 1 cut(s) 466
FbaI TGATCA 1 cut(s) 1072
FblI GTMKAC 1 cut(s) 15
Fnu4HI GCNGC 5 cut(s) 233, 253, 484, 600, 730
FokI GGATG 5 cut(s) 107, 1030, 1058, 1082, 1152
FriOI GRGCYC 2 cut(s) 312, 347
Fsp4HI GCNGC 5 cut(s) 233, 253, 484, 600, 730
FspBI CTAG 1 cut(s) 134
GlaI GCGC 7 cut(s) 293, 603, 689, 721, 753, 792, 920
GluI GCNGC 5 cut(s) 233, 253, 484, 600, 730
GsaI CCCAGC 1 cut(s) 309
HaeIII GGCC 2 cut(s) 310, 504
HapII CCGG 5 cut(s) 201, 226, 419, 434, 656
HgaI GACGC 2 cut(s) 344, 520
HhaI GCGC 7 cut(s) 294, 604, 690, 722, 754, 793, 921
Hin1I GRCGYC 1 cut(s) 531
Hin1II CATG 6 cut(s) 200, 472, 781, 872, 898, 926
Hin6I GCGC 7 cut(s) 292, 602, 688, 720, 752, 791, 919
HinP1I GCGC 7 cut(s) 292, 602, 688, 720, 752, 791, 919
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HinfI GANTC 5 cut(s) 185, 216, 428, 629, 1174
HpaII CCGG 5 cut(s) 201, 226, 419, 434, 656
HphI GGTGA 5 cut(s) 575, 691, 793, 986, 1073
Hpy166II GTNNAC 2 cut(s) 16, 139
Hpy188I TCNGA 4 cut(s) 10, 544, 1116, 1162
Hpy188III TCNNGA 1 cut(s) 191
Hpy8I GTNNAC 2 cut(s) 16, 139
Hpy99I CGWCG 7 cut(s) 252, 284, 338, 397, 536, 544, 862
HpyAV CCTTC 6 cut(s) 60, 570, 631, 948, 1015, 1060
HpyCH4IV ACGT 2 cut(s) 392, 857
HpyF10VI GCNNNNNNNGC 6 cut(s) 252, 291, 316, 726, 760, 1178
HpyF3I CTNAG 2 cut(s) 549, 864
HpySE526I ACGT 2 cut(s) 392, 857
Hsp92I GRCGYC 1 cut(s) 531
Hsp92II CATG 6 cut(s) 200, 472, 781, 872, 898, 926
HspAI GCGC 7 cut(s) 292, 602, 688, 720, 752, 791, 919
KpnI GGTACC 1 cut(s) 841
Ksp22I TGATCA 1 cut(s) 1072
Kzo9I GATC 7 cut(s) 441, 489, 564, 738, 819, 1072, 1162
LguI GCTCTTC 1 cut(s) 93
LmnI GCTCC 3 cut(s) 436, 760, 1060
LweI GCATC 5 cut(s) 772, 868, 1036, 1107, 1117
MaeI CTAG 1 cut(s) 134
MaeII ACGT 2 cut(s) 392, 857
MaeIII GTNAC 1 cut(s) 494
MalI GATC 7 cut(s) 443, 491, 566, 740, 821, 1074, 1164
MboI GATC 7 cut(s) 441, 489, 564, 738, 819, 1072, 1162
MfeI CAATTG 1 cut(s) 1010
MflI RGATCY 1 cut(s) 441
MhlI GDGCHC 2 cut(s) 312, 347
MluCI AATT 5 cut(s) 21, 948, 996, 1010, 1156
MlyI GAGTC 3 cut(s) 210, 437, 623
MmeI TCCRAC 2 cut(s) 100, 522
MseI TTAA 3 cut(s) 60, 113, 351
MspCI CTTAAG 1 cut(s) 59
MspI CCGG 5 cut(s) 201, 226, 419, 434, 656
MspR9I CCNGG 4 cut(s) 419, 657, 842, 956
MunI CAATTG 1 cut(s) 1010
MvaI CCWGG 2 cut(s) 842, 956
MvnI CGCG 9 cut(s) 252, 292, 338, 604, 677, 690, 722, 754, 793
MwoI GCNNNNNNNGC 6 cut(s) 252, 291, 316, 726, 760, 1178
NciI CCSGG 2 cut(s) 419, 657
NdeII GATC 7 cut(s) 441, 489, 564, 738, 819, 1072, 1162
NlaIII CATG 6 cut(s) 200, 472, 781, 872, 898, 926
NlaIV GGNNCC 5 cut(s) 310, 311, 654, 714, 839
NmuCI GTSAC 1 cut(s) 494
NspI RCATGY 1 cut(s) 200
PaeI GCATGC 1 cut(s) 200
PciSI GCTCTTC 1 cut(s) 93
PcsI WCGNNNNNNNCGW 2 cut(s) 271, 624
PfeI GAWTC 2 cut(s) 185, 1174
PflFI GACNNNGTC 1 cut(s) 530
PfoI TCCNGGA 1 cut(s) 655
PkrI GCNGC 5 cut(s) 234, 254, 485, 601, 731
PleI GAGTC 3 cut(s) 210, 436, 623
PpsI GAGTC 3 cut(s) 210, 436, 623
Psp6I CCWGG 2 cut(s) 840, 954
PspFI CCCAGC 1 cut(s) 305
PspGI CCWGG 2 cut(s) 840, 954
PspN4I GGNNCC 5 cut(s) 310, 311, 654, 714, 839
PspOMI GGGCCC 1 cut(s) 308
PspPI GGNCC 4 cut(s) 308, 309, 653, 1148
PstNI CAGNNNCTG 1 cut(s) 1120
PsuI RGATCY 1 cut(s) 441
PsyI GACNNNGTC 1 cut(s) 530
RsaI GTAC 3 cut(s) 770, 839, 884
RsaNI GTAC 3 cut(s) 769, 838, 883
SalI GTCGAC 1 cut(s) 14
SapI GCTCTTC 1 cut(s) 93
SaqAI TTAA 3 cut(s) 60, 113, 351
SatI GCNGC 5 cut(s) 233, 253, 484, 600, 730
Sau3AI GATC 7 cut(s) 441, 489, 564, 738, 819, 1072, 1162
Sau96I GGNCC 4 cut(s) 308, 309, 653, 1148
SchI GAGTC 3 cut(s) 210, 437, 623
ScrFI CCNGG 4 cut(s) 419, 657, 842, 956
SduI GDGCHC 2 cut(s) 312, 347
SfaNI GCATC 5 cut(s) 772, 868, 1036, 1107, 1117
SinI GGWCC 2 cut(s) 653, 1148
SmlI CTYRAG 3 cut(s) 59, 189, 1136
SmoI CTYRAG 3 cut(s) 59, 189, 1136
SphI GCATGC 1 cut(s) 200
Sse9I AATT 5 cut(s) 21, 948, 996, 1010, 1156
SspMI CTAG 1 cut(s) 134
StyD4I CCNGG 4 cut(s) 417, 655, 840, 954
StyI CCWWGG 2 cut(s) 1050, 1092
TaiI ACGT 2 cut(s) 395, 860
TaqI TCGA 3 cut(s) 15, 563, 627
TasI AATT 5 cut(s) 21, 948, 996, 1010, 1156
TauI GCSGC 5 cut(s) 235, 255, 486, 602, 732
TfiI GAWTC 2 cut(s) 185, 1174
Tru1I TTAA 3 cut(s) 60, 113, 351
Tru9I TTAA 3 cut(s) 60, 113, 351
TscAI CASTG 2 cut(s) 527, 596
TseFI GTSAC 1 cut(s) 494
Tsp45I GTSAC 1 cut(s) 494
TspDTI ATGAA 4 cut(s) 17, 111, 436, 1034
TspGWI ACGGA 2 cut(s) 482, 528
TspRI CASTG 2 cut(s) 527, 596
Tth111I GACNNNGTC 1 cut(s) 530
Vha464I CTTAAG 1 cut(s) 59
VpaK11BI GGWCC 2 cut(s) 653, 1148
XceI RCATGY 1 cut(s) 200
XcmI CCANNNNNNNNNTGG 1 cut(s) 302
XmiI GTMKAC 1 cut(s) 15
XspI CTAG 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.