Rorug05G0558600

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003321
Physical Location & Seq
Forward (+)
74792282 .. 74793586
1305 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0558600.1

Sequence Viewer

Length: 549 bp
ATGGCAGCTGCTATTCGGTCTATGCTCGGAAAGTCAGGAAATAGAATATCCTCAGTAGCAAACATGGTGGCTATGGGGGATTCTGAAATTAGAACCCTAAATATTAATCATAATCTAAATTTGGGTTATTCCTCTCATCACCCTCTTGAATCTTTTTCGGTAGTAAACCGTCCCAGCAATATTCGAAGGCCTAAGAAGCTGGAACCTAAACACCCGGAAATTCCAAGAAAGTTATATGGAGCAAGTCTTTTCTCTCGCTCCTCCAGATATTGTACCAGGGTTGTACTTTATTCACATCTCGCACTGGAAGAATTCAACAAGCAAAAGAATGACCAACTGCAATTTGTCAAAGTGGTGAAGGCATTTAACCAGTGGAGTGGTGGTTGGAAGAATCATTTGCTAACATTGGAGGCAATTGATGCTGATAATGTGATCAAAACTTACCAAGCTATAGTCGTGCATTGGAAACCTAAACATGATTTAAGTCCCTCCAGAGCATTAGAGTTTTCCTCGAGCCCTAGTAGAAGCCTTACATGGGTTCACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

182

Amino Acids

20.7

Weight (kDa)

10.13

Isoelectric Point (pI)

55.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQAPI PF16845 94 - 154 3.3e-09 Aspartic acid proteinase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 3 cut(s) 118, 219, 311
AfaI GTAC 2 cut(s) 274, 285
AfiI CCNNNNNNNGG 1 cut(s) 535
AgsI TTSAA 2 cut(s) 149, 316
AjnI CCWGG 1 cut(s) 275
AluBI AGCT 3 cut(s) 8, 199, 449
AluI AGCT 3 cut(s) 8, 199, 449
Ama87I CYCGRG 1 cut(s) 511
AoxI GGCC 1 cut(s) 188
ApeKI GCWGC 2 cut(s) 5, 8
ApoI RAATTY 3 cut(s) 118, 219, 311
AseI ATTAAT 1 cut(s) 105
AsuC2I CCSGG 1 cut(s) 215
AsuHPI GGTGA 2 cut(s) 131, 367
AsuII TTCGAA 1 cut(s) 184
AvaI CYCGRG 1 cut(s) 511
BanII GRGCYC 1 cut(s) 518
BbvI GCAGC 1 cut(s) 17
BciT130I CCWGG 1 cut(s) 277
BclI TGATCA 1 cut(s) 432
BcnI CCSGG 1 cut(s) 215
BfaI CTAG 1 cut(s) 519
BfmI CTRYAG 1 cut(s) 450
BisI GCNGC 2 cut(s) 6, 9
BlsI GCNGC 2 cut(s) 7, 10
Bme1390I CCNGG 2 cut(s) 215, 277
BmeT110I CYCGRG 1 cut(s) 511
BmiI GGNNCC 1 cut(s) 204
BmrFI CCNGG 2 cut(s) 215, 277
BmsI GCATC 1 cut(s) 409
BplI GAGNNNNNCTC 2 cut(s) 494, 526
BpmI CTGGAG 2 cut(s) 247, 475
Bpu14I TTCGAA 1 cut(s) 184
BpuMI CCSGG 1 cut(s) 215
BsaJI CCNNGG 1 cut(s) 276
BsaXI ACNNNNNCTCC 2 cut(s) 367, 397
Bsc4I CCNNNNNNNGG 1 cut(s) 535
Bse1I ACTGG 2 cut(s) 309, 370
BseBI CCWGG 1 cut(s) 277
BseDI CCNNGG 1 cut(s) 276
BseLI CCNNNNNNNGG 1 cut(s) 535
BseMII CTCAG 1 cut(s) 66
BseNI ACTGG 2 cut(s) 309, 370
BseRI GAGGAG 1 cut(s) 250
BseXI GCAGC 1 cut(s) 17
BseYI CCCAGC 1 cut(s) 173
BshFI GGCC 1 cut(s) 190
BsiHKCI CYCGRG 1 cut(s) 511
BsiSI CCGG 1 cut(s) 215
BslFI GGGAC 2 cut(s) 156, 471
BslI CCNNNNNNNGG 1 cut(s) 535
BsmFI GGGAC 2 cut(s) 156, 471
BsnI GGCC 1 cut(s) 190
BsoBI CYCGRG 1 cut(s) 511
Bsp119I TTCGAA 1 cut(s) 184
Bsp1286I GDGCHC 1 cut(s) 518
Bsp143I GATC 1 cut(s) 432
BspANI GGCC 1 cut(s) 190
BspCNI CTCAG 1 cut(s) 65
BspLI GGNNCC 1 cut(s) 204
BspT104I TTCGAA 1 cut(s) 184
BsrI ACTGG 2 cut(s) 309, 370
BssECI CCNNGG 1 cut(s) 276
BssMI GATC 1 cut(s) 432
Bst2UI CCWGG 1 cut(s) 277
Bst4CI ACNGT 1 cut(s) 170
BstAPI GCANNNNNTGC 1 cut(s) 419
BstBI TTCGAA 1 cut(s) 184
BstDEI CTNAG 2 cut(s) 52, 192
BstKTI GATC 1 cut(s) 435
BstMBI GATC 1 cut(s) 432
BstMWI GCNNNNNNNGC 2 cut(s) 196, 419
BstNI CCWGG 1 cut(s) 277
BstSCI CCNGG 2 cut(s) 213, 275
BstSFI CTRYAG 1 cut(s) 450
BstV1I GCAGC 1 cut(s) 17
BstXI CCANNNNNNTGG 1 cut(s) 377
BsuRI GGCC 1 cut(s) 190
BtsIMutI CAGTG 2 cut(s) 302, 377
Csp6I GTAC 2 cut(s) 273, 284
CspCI CAANNNNNGTGG 2 cut(s) 48, 83
CviAII CATG 3 cut(s) 64, 476, 534
CviJI RGCY 7 cut(s) 8, 71, 190, 199, 449, 516, 528
CviKI_1 RGCY 7 cut(s) 8, 71, 190, 199, 449, 516, 528
CviQI GTAC 2 cut(s) 273, 284
DdeI CTNAG 2 cut(s) 52, 192
DpnI GATC 1 cut(s) 434
DpnII GATC 1 cut(s) 432
Eco147I AGGCCT 1 cut(s) 190
Eco24I GRGCYC 1 cut(s) 518
Eco88I CYCGRG 1 cut(s) 511
EcoRI GAATTC 1 cut(s) 311
EcoRII CCWGG 1 cut(s) 275
EcoT38I GRGCYC 1 cut(s) 518
FaeI CATG 3 cut(s) 67, 479, 537
FaiI YATR 9 cut(s) 23, 65, 74, 111, 235, 237, 452, 477, 535
FaqI GGGAC 2 cut(s) 156, 471
FatI CATG 3 cut(s) 63, 475, 533
FbaI TGATCA 1 cut(s) 432
Fnu4HI GCNGC 2 cut(s) 6, 9
FriOI GRGCYC 1 cut(s) 518
Fsp4HI GCNGC 2 cut(s) 6, 9
FspBI CTAG 1 cut(s) 519
GluI GCNGC 2 cut(s) 6, 9
GsaI CCCAGC 1 cut(s) 177
GsuI CTGGAG 2 cut(s) 247, 475
HaeIII GGCC 1 cut(s) 190
HapII CCGG 1 cut(s) 215
Hin1II CATG 3 cut(s) 67, 479, 537
HinfI GANTC 3 cut(s) 80, 149, 391
HpaII CCGG 1 cut(s) 215
HphI GGTGA 2 cut(s) 131, 367
Hpy166II GTNNAC 2 cut(s) 166, 541
Hpy188I TCNGA 2 cut(s) 29, 85
Hpy188III TCNNGA 4 cut(s) 36, 146, 264, 492
Hpy8I GTNNAC 2 cut(s) 166, 541
HpyAV CCTTC 2 cut(s) 180, 352
HpyCH4III ACNGT 1 cut(s) 170
HpyCH4V TGCA 2 cut(s) 340, 460
HpyF10VI GCNNNNNNNGC 2 cut(s) 196, 419
HpyF3I CTNAG 2 cut(s) 52, 192
Hsp92II CATG 3 cut(s) 67, 479, 537
Ksp22I TGATCA 1 cut(s) 432
Kzo9I GATC 1 cut(s) 432
LmnI GCTCC 2 cut(s) 239, 263
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 1 cut(s) 409
MaeI CTAG 1 cut(s) 519
MalI GATC 1 cut(s) 434
MboI GATC 1 cut(s) 432
MboII GAAGA 2 cut(s) 320, 400
MfeI CAATTG 1 cut(s) 414
MhlI GDGCHC 1 cut(s) 518
MluCI AATT 6 cut(s) 87, 118, 219, 311, 341, 414
MmeI TCCRAC 1 cut(s) 365
MnlI CCTC 7 cut(s) 61, 142, 153, 271, 403, 499, 520
MseI TTAA 3 cut(s) 105, 366, 482
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 1 cut(s) 215
MspR9I CCNGG 2 cut(s) 215, 277
MunI CAATTG 1 cut(s) 414
MvaI CCWGG 1 cut(s) 277
MwoI GCNNNNNNNGC 2 cut(s) 196, 419
NciI CCSGG 1 cut(s) 215
NdeII GATC 1 cut(s) 432
NlaIII CATG 3 cut(s) 67, 479, 537
NlaIV GGNNCC 1 cut(s) 204
NspV TTCGAA 1 cut(s) 184
PaeR7I CTCGAG 1 cut(s) 511
PceI AGGCCT 1 cut(s) 190
PfeI GAWTC 3 cut(s) 80, 149, 391
PkrI GCNGC 2 cut(s) 7, 10
PshBI ATTAAT 1 cut(s) 105
Psp6I CCWGG 1 cut(s) 275
PspFI CCCAGC 1 cut(s) 173
PspGI CCWGG 1 cut(s) 275
PspN4I GGNNCC 1 cut(s) 204
PspXI VCTCGAGB 1 cut(s) 511
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 2 cut(s) 274, 285
RsaNI GTAC 2 cut(s) 273, 284
SaqAI TTAA 3 cut(s) 105, 366, 482
SatI GCNGC 2 cut(s) 6, 9
Sau3AI GATC 1 cut(s) 432
ScrFI CCNGG 2 cut(s) 215, 277
SduI GDGCHC 1 cut(s) 518
SetI ASST 5 cut(s) 10, 201, 208, 451, 472
SfaNI GCATC 1 cut(s) 409
SfcI CTRYAG 1 cut(s) 450
Sfr274I CTCGAG 1 cut(s) 511
SfuI TTCGAA 1 cut(s) 184
SlaI CTCGAG 1 cut(s) 511
SmlI CTYRAG 1 cut(s) 511
SmoI CTYRAG 1 cut(s) 511
Sse9I AATT 6 cut(s) 87, 118, 219, 311, 341, 414
SseBI AGGCCT 1 cut(s) 190
SspI AATATT 2 cut(s) 103, 181
SspMI CTAG 1 cut(s) 519
StuI AGGCCT 1 cut(s) 190
StyD4I CCNGG 2 cut(s) 213, 275
TaaI ACNGT 1 cut(s) 170
TaqI TCGA 2 cut(s) 184, 512
TaqII GACCGA 1 cut(s) 6
TasI AATT 6 cut(s) 87, 118, 219, 311, 341, 414
TatI WGTACW 1 cut(s) 283
TfiI GAWTC 3 cut(s) 80, 149, 391
Tru1I TTAA 3 cut(s) 105, 366, 482
Tru9I TTAA 3 cut(s) 105, 366, 482
TscAI CASTG 2 cut(s) 309, 377
TseI GCWGC 2 cut(s) 5, 8
TspRI CASTG 2 cut(s) 309, 377
VspI ATTAAT 1 cut(s) 105
XapI RAATTY 3 cut(s) 118, 219, 311
XcmI CCANNNNNNNNNTGG 1 cut(s) 377
XhoI CTCGAG 1 cut(s) 511
XspI CTAG 1 cut(s) 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.